LOCUS       DARBEG010000001       126320 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000001 DARBEG010000000
VERSION     DARBEG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 126320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 126320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..126320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.001"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..604
                     /locus_tag="SCK20_000001"
     CDS             212..604
                     /locus_tag="SCK20_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1144102.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="SCK20_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="SCK20_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG1144103.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="SCK20_000003"
     CDS             3165..4775
                     /locus_tag="SCK20_000003"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG1144104.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="SCK20_000004"
     CDS             5067..5936
                     /locus_tag="SCK20_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG1144105.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="SCK20_000005"
     CDS             6101..7372
                     /locus_tag="SCK20_000005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1144106.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="SCK20_000006"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="SCK20_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG1144107.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="SCK20_000007"
     CDS             8763..9026
                     /locus_tag="SCK20_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG1144108.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="SCK20_000008"
     CDS             9471..9920
                     /locus_tag="SCK20_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1144109.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="SCK20_000009"
     CDS             9977..11395
                     /locus_tag="SCK20_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1144110.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="SCK20_000010"
     CDS             11395..12255
                     /locus_tag="SCK20_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG1144111.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="SCK20_000011"
     CDS             12429..13202
                     /locus_tag="SCK20_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1144112.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="SCK20_000012"
     CDS             13349..13714
                     /locus_tag="SCK20_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG1144113.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="SCK20_000013"
     CDS             complement(13862..15070)
                     /locus_tag="SCK20_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1144114.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="SCK20_000014"
     CDS             complement(15089..15367)
                     /locus_tag="SCK20_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144115.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="SCK20_000015"
     CDS             15521..16216
                     /locus_tag="SCK20_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1144116.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="SCK20_000016"
     CDS             complement(16658..17455)
                     /locus_tag="SCK20_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG1144117.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="SCK20_000017"
     CDS             complement(17452..18636)
                     /locus_tag="SCK20_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1144118.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="SCK20_000018"
     CDS             complement(18661..19908)
                     /locus_tag="SCK20_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1144119.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="SCK20_000019"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="SCK20_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG1144120.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="SCK20_000020"
     CDS             21888..22202
                     /locus_tag="SCK20_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG1144121.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="SCK20_000021"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="SCK20_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG1144122.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="SCK20_000022"
     CDS             22887..23531
                     /locus_tag="SCK20_000022"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG1144123.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="SCK20_000023"
     CDS             23546..23875
                     /locus_tag="SCK20_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG1144124.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="SCK20_000024"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="SCK20_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG1144125.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="SCK20_000025"
     CDS             24863..25687
                     /locus_tag="SCK20_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG1144126.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="SCK20_000026"
     CDS             25680..26027
                     /locus_tag="SCK20_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG1144127.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="SCK20_000027"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="SCK20_000027"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG1144128.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="SCK20_000028"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="SCK20_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG1144129.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCK20_000029"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCK20_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG1144130.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCK20_000030"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCK20_000030"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG1144131.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="SCK20_000031"
     CDS             31657..32451
                     /locus_tag="SCK20_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144132.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="SCK20_000032"
     CDS             32441..34483
                     /locus_tag="SCK20_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144133.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="SCK20_000033"
     CDS             34764..34913
                     /locus_tag="SCK20_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG1144134.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="SCK20_000034"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="SCK20_000034"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG1144135.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="SCK20_000035"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="SCK20_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG1144136.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="SCK20_000036"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="SCK20_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG1144137.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="SCK20_000037"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="SCK20_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG1144138.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="SCK20_000038"
     CDS             39330..40412
                     /locus_tag="SCK20_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG1144139.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="SCK20_000039"
     CDS             40412..41215
                     /locus_tag="SCK20_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1144140.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="SCK20_000040"
     CDS             41285..42025
                     /locus_tag="SCK20_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1144141.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="SCK20_000041"
     CDS             42036..43040
                     /locus_tag="SCK20_000041"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG1144142.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44929)
                     /locus_tag="SCK20_000042"
     CDS             complement(43085..44929)
                     /locus_tag="SCK20_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144143.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45141..45659
                     /locus_tag="SCK20_000043"
     CDS             45141..45659
                     /locus_tag="SCK20_000043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1144144.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45787..46470
                     /locus_tag="SCK20_000044"
     CDS             45787..46470
                     /locus_tag="SCK20_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG1144145.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46779..46852
                     /locus_tag="SCK20_000045"
     tRNA            46779..46852
                     /locus_tag="SCK20_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46813..46815,aa:Arg,seq:tct)
     gene            47011..47370
                     /locus_tag="SCK20_000046"
     CDS             47011..47370
                     /locus_tag="SCK20_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG1144146.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47493..49124
                     /locus_tag="SCK20_000047"
     CDS             47493..49124
                     /locus_tag="SCK20_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG1144147.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49186..49827)
                     /locus_tag="SCK20_000048"
     CDS             complement(49186..49827)
                     /locus_tag="SCK20_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1144148.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50013..50089
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50109..50417
                     /gene="rplU"
                     /locus_tag="SCK20_000049"
     CDS             50109..50417
                     /gene="rplU"
                     /locus_tag="SCK20_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG1144149.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50444..50788
                     /locus_tag="SCK20_000050"
     CDS             50444..50788
                     /locus_tag="SCK20_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG1144150.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50801..51094
                     /gene="rpmA"
                     /locus_tag="SCK20_000051"
     CDS             50801..51094
                     /gene="rpmA"
                     /locus_tag="SCK20_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG1144151.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51528..54335
                     /locus_tag="SCK20_000052"
     CDS             51528..54335
                     /locus_tag="SCK20_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1144152.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54526..55017
                     /locus_tag="SCK20_000053"
     CDS             54526..55017
                     /locus_tag="SCK20_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1144153.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55120..56109
                     /gene="manX"
                     /locus_tag="SCK20_000054"
     CDS             55120..56109
                     /gene="manX"
                     /locus_tag="SCK20_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG1144154.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56142..56945
                     /locus_tag="SCK20_000055"
     CDS             56142..56945
                     /locus_tag="SCK20_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG1144155.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56966..57880
                     /locus_tag="SCK20_000056"
     CDS             56966..57880
                     /locus_tag="SCK20_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG1144156.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57963..58337
                     /locus_tag="SCK20_000057"
     CDS             57963..58337
                     /locus_tag="SCK20_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG1144157.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58476..58721
                     /locus_tag="SCK20_000058"
     CDS             58476..58721
                     /locus_tag="SCK20_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1144158.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58893..59957
                     /locus_tag="SCK20_000059"
     CDS             58893..59957
                     /locus_tag="SCK20_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1144159.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60203..60628
                     /locus_tag="SCK20_000060"
     CDS             60203..60628
                     /locus_tag="SCK20_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1144160.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60621..61070
                     /gene="nusB"
                     /locus_tag="SCK20_000061"
     CDS             60621..61070
                     /gene="nusB"
                     /locus_tag="SCK20_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG1144161.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61167..62021
                     /locus_tag="SCK20_000062"
     CDS             61167..62021
                     /locus_tag="SCK20_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG1144162.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62022..63359
                     /locus_tag="SCK20_000063"
     CDS             62022..63359
                     /locus_tag="SCK20_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG1144163.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63364..63588
                     /locus_tag="SCK20_000064"
     CDS             63364..63588
                     /locus_tag="SCK20_000064"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG1144164.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63590..64480
                     /locus_tag="SCK20_000065"
     CDS             63590..64480
                     /locus_tag="SCK20_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1144165.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64489..65307
                     /locus_tag="SCK20_000066"
     CDS             64489..65307
                     /locus_tag="SCK20_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG1144166.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65414..65863
                     /locus_tag="SCK20_000067"
     CDS             65414..65863
                     /locus_tag="SCK20_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1144167.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65881..67563
                     /gene="recN"
                     /locus_tag="SCK20_000068"
     CDS             65881..67563
                     /gene="recN"
                     /locus_tag="SCK20_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG1144168.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67618..67734)
                     /locus_tag="SCK20_000069"
     CDS             complement(67618..67734)
                     /locus_tag="SCK20_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG1144169.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67807..68763)
                     /locus_tag="SCK20_000070"
     CDS             complement(67807..68763)
                     /locus_tag="SCK20_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1144170.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68934..69302
                     /locus_tag="SCK20_000071"
     CDS             68934..69302
                     /locus_tag="SCK20_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG1144171.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69472..69903
                     /gene="mraZ"
                     /locus_tag="SCK20_000072"
     CDS             69472..69903
                     /gene="mraZ"
                     /locus_tag="SCK20_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG1144172.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69924..70883
                     /gene="rsmH"
                     /locus_tag="SCK20_000073"
     CDS             69924..70883
                     /gene="rsmH"
                     /locus_tag="SCK20_000073"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG1144173.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70888..71289
                     /gene="ftsL"
                     /locus_tag="SCK20_000074"
     CDS             70888..71289
                     /gene="ftsL"
                     /locus_tag="SCK20_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG1144174.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71290..73482
                     /locus_tag="SCK20_000075"
     CDS             71290..73482
                     /locus_tag="SCK20_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1144175.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73508..74470
                     /locus_tag="SCK20_000076"
     CDS             73508..74470
                     /locus_tag="SCK20_000076"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG1144176.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74484..75845
                     /locus_tag="SCK20_000077"
     CDS             74484..75845
                     /locus_tag="SCK20_000077"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG1144177.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75871..76959
                     /gene="murG"
                     /locus_tag="SCK20_000078"
     CDS             75871..76959
                     /gene="murG"
                     /locus_tag="SCK20_000078"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG1144178.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76974..78194
                     /locus_tag="SCK20_000079"
     CDS             76974..78194
                     /locus_tag="SCK20_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG1144179.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78350..79675
                     /gene="ftsA"
                     /locus_tag="SCK20_000080"
     CDS             78350..79675
                     /gene="ftsA"
                     /locus_tag="SCK20_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG1144180.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79698..80939
                     /gene="ftsZ"
                     /locus_tag="SCK20_000081"
     CDS             79698..80939
                     /gene="ftsZ"
                     /locus_tag="SCK20_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG1144181.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80956..81633
                     /locus_tag="SCK20_000082"
     CDS             80956..81633
                     /locus_tag="SCK20_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG1144182.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81647..82246
                     /locus_tag="SCK20_000083"
     CDS             81647..82246
                     /locus_tag="SCK20_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG1144183.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82261..82548
                     /locus_tag="SCK20_000084"
     CDS             82261..82548
                     /locus_tag="SCK20_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG1144184.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82597..83379
                     /locus_tag="SCK20_000085"
     CDS             82597..83379
                     /locus_tag="SCK20_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG1144185.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83400..84107
                     /locus_tag="SCK20_000086"
     CDS             83400..84107
                     /locus_tag="SCK20_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG1144186.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84369..87167
                     /gene="ileS"
                     /locus_tag="SCK20_000087"
     CDS             84369..87167
                     /gene="ileS"
                     /locus_tag="SCK20_000087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG1144187.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87216..88742)
                     /locus_tag="SCK20_000088"
     CDS             complement(87216..88742)
                     /locus_tag="SCK20_000088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG1144188.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88757..89404)
                     /locus_tag="SCK20_000089"
     CDS             complement(88757..89404)
                     /locus_tag="SCK20_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG1144189.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89588..89917)
                     /locus_tag="SCK20_000090"
     CDS             complement(89588..89917)
                     /locus_tag="SCK20_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG1144190.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90094..90822
                     /gene="sfsA"
                     /locus_tag="SCK20_000091"
     CDS             90094..90822
                     /gene="sfsA"
                     /locus_tag="SCK20_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG1144191.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90838..91851
                     /locus_tag="SCK20_000092"
     CDS             90838..91851
                     /locus_tag="SCK20_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1144192.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91851..93128
                     /locus_tag="SCK20_000093"
     CDS             91851..93128
                     /locus_tag="SCK20_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1144193.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93191..95893
                     /locus_tag="SCK20_000094"
     CDS             93191..95893
                     /locus_tag="SCK20_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1144194.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96045..96362
                     /locus_tag="SCK20_000095"
     CDS             96045..96362
                     /locus_tag="SCK20_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1144195.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96392..96712
                     /locus_tag="SCK20_000096"
     CDS             96392..96712
                     /locus_tag="SCK20_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1144196.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96820..98280
                     /locus_tag="SCK20_000097"
     CDS             96820..98280
                     /locus_tag="SCK20_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1144197.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98348..98569
                     /locus_tag="SCK20_000098"
     CDS             98348..98569
                     /locus_tag="SCK20_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144198.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98600..98830
                     /locus_tag="SCK20_000099"
     CDS             98600..98830
                     /locus_tag="SCK20_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG1144199.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKEKKQRRKITNGNHEKIKYSSIVFLR"
     gene            98781..99194
                     /locus_tag="SCK20_000100"
     CDS             98781..99194
                     /locus_tag="SCK20_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG1144200.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99317..100498
                     /locus_tag="SCK20_000101"
     CDS             99317..100498
                     /locus_tag="SCK20_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1144201.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100569..100733)
                     /locus_tag="SCK20_000102"
     CDS             complement(100569..100733)
                     /locus_tag="SCK20_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144202.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100887..100960
                     /locus_tag="SCK20_000103"
     tRNA            100887..100960
                     /locus_tag="SCK20_000103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100921..100923,aa:Arg,seq:cct)
     gene            complement(100997..101197)
                     /locus_tag="SCK20_000104"
     CDS             complement(100997..101197)
                     /locus_tag="SCK20_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1144203.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(101502..102734)
                     /locus_tag="SCK20_000105"
     CDS             complement(101502..102734)
                     /locus_tag="SCK20_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG1144204.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            102988..103557
                     /locus_tag="SCK20_000106"
     CDS             102988..103557
                     /locus_tag="SCK20_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1144205.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(103735..104175)
                     /locus_tag="SCK20_000107"
     CDS             complement(103735..104175)
                     /locus_tag="SCK20_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG1144206.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            104333..105097
                     /gene="map"
                     /locus_tag="SCK20_000108"
     CDS             104333..105097
                     /gene="map"
                     /locus_tag="SCK20_000108"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG1144207.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            105129..106052
                     /locus_tag="SCK20_000109"
     CDS             105129..106052
                     /locus_tag="SCK20_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG1144208.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            106128..107267
                     /locus_tag="SCK20_000110"
     CDS             106128..107267
                     /locus_tag="SCK20_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG1144209.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            107260..108060
                     /locus_tag="SCK20_000111"
     CDS             107260..108060
                     /locus_tag="SCK20_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1144210.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            108060..108887
                     /locus_tag="SCK20_000112"
     CDS             108060..108887
                     /locus_tag="SCK20_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1144211.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            108865..109599
                     /locus_tag="SCK20_000113"
     CDS             108865..109599
                     /locus_tag="SCK20_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1144212.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            109699..110565
                     /gene="rfbA"
                     /locus_tag="SCK20_000114"
     CDS             109699..110565
                     /gene="rfbA"
                     /locus_tag="SCK20_000114"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG1144213.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            110579..111151
                     /gene="rfbC"
                     /locus_tag="SCK20_000115"
     CDS             110579..111151
                     /gene="rfbC"
                     /locus_tag="SCK20_000115"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG1144214.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            111173..112201
                     /gene="rfbB"
                     /locus_tag="SCK20_000116"
     CDS             111173..112201
                     /gene="rfbB"
                     /locus_tag="SCK20_000116"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG1144215.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            112299..113150
                     /gene="rfbD"
                     /locus_tag="SCK20_000117"
     CDS             112299..113150
                     /gene="rfbD"
                     /locus_tag="SCK20_000117"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG1144216.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            113185..115218
                     /locus_tag="SCK20_000118"
     CDS             113185..115218
                     /locus_tag="SCK20_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144217.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            115262..116542
                     /locus_tag="SCK20_000119"
     CDS             115262..116542
                     /locus_tag="SCK20_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG1144218.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            116752..117558
                     /locus_tag="SCK20_000120"
     CDS             116752..117558
                     /locus_tag="SCK20_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144219.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            117570..118790
                     /locus_tag="SCK20_000121"
     CDS             117570..118790
                     /locus_tag="SCK20_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144220.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            118780..120366
                     /locus_tag="SCK20_000122"
     CDS             118780..120366
                     /locus_tag="SCK20_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1144221.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            120405..122543
                     /locus_tag="SCK20_000123"
     CDS             120405..122543
                     /locus_tag="SCK20_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1144222.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(122911..123894)
                     /locus_tag="SCK20_000124"
     CDS             complement(122911..123894)
                     /locus_tag="SCK20_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG1144223.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            124272..125663
                     /locus_tag="SCK20_000125"
     CDS             124272..125663
                     /locus_tag="SCK20_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1144224.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            125679..>126320
                     /locus_tag="SCK20_000126"
     CDS             125679..>126320
                     /locus_tag="SCK20_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1144225.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYI"
BASE COUNT        41009 a        21592 c        26594 g        37125 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt agaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    44941 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    45001 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    45061 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    45121 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    45181 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    45241 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    45301 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    45361 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    45421 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    45481 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    45541 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    45601 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    45661 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    45721 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    45781 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    45841 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    45901 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    45961 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    46021 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    46081 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    46141 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    46201 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    46261 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    46321 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    46381 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    46441 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    46501 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    46561 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    46621 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    46681 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    46741 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    46801 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    46861 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    46921 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    46981 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    47041 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    47101 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    47161 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    47221 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    47281 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    47341 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    47401 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    47461 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    47521 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    47581 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    47641 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    47701 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    47761 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    47821 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    47881 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    47941 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    48001 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    48061 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    48121 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    48181 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    48241 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    48301 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    48361 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    48421 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    48481 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    48541 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    48601 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    48661 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    48721 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    48781 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    48841 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    48901 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    48961 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    49021 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    49081 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    49141 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    49201 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    49261 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    49321 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    49381 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    49441 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    49501 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    49561 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    49621 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    49681 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    49741 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    49801 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    49861 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    49921 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    49981 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    50041 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    50101 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    50161 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    50221 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    50281 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    50341 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    50401 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    50461 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    50521 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    50581 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    50641 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    50701 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    50761 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    50821 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    50881 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    50941 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    51001 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    51061 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    51121 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    51181 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    51241 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    51301 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    51361 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    51421 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    51481 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    51541 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    51601 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    51661 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    51721 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    51781 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    51841 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    51901 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    51961 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    52021 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    52081 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    52141 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    52201 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    52261 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    52321 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    52381 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    52441 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    52501 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    52561 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    52621 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    52681 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    52741 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    52801 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    52861 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    52921 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    52981 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    53041 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    53101 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    53161 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    53221 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    53281 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    53341 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    53401 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    53461 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    53521 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    53581 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    53641 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    53701 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    53761 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    53821 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    53881 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    53941 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    54001 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    54061 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    54121 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    54181 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    54241 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    54301 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    54361 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    54421 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    54481 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    54541 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    54601 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    54661 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    54721 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    54781 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    54841 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    54901 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    54961 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    55021 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    55081 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    55141 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    55201 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    55261 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    55321 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    55381 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    55441 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    55501 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    55561 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    55621 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    55681 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    55741 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    55801 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    55861 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    55921 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    55981 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    56041 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    56101 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    56161 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    56221 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    56281 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    56341 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    56401 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    56461 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    56521 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    56581 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    56641 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    56701 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    56761 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    56821 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    56881 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    56941 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    57001 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    57061 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    57121 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    57181 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    57241 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    57301 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    57361 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    57421 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    57481 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    57541 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    57601 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    57661 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    57721 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    57781 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    57841 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    57901 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    57961 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    58021 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    58081 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    58141 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    58201 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    58261 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    58321 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    58381 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    58441 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    58501 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    58561 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    58621 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    58681 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    58741 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    58801 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    58861 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    58921 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    58981 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    59041 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    59101 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    59161 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    59221 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    59281 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    59341 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    59401 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    59461 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    59521 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    59581 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    59641 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    59701 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    59761 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    59821 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    59881 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    59941 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    60001 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    60061 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    60121 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    60181 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    60241 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    60301 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    60361 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    60421 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    60481 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    60541 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    60601 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    60661 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    60721 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    60781 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    60841 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    60901 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    60961 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    61021 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    61081 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    61141 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    61201 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    61261 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    61321 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    61381 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    61441 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    61501 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    61561 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    61621 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    61681 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    61741 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    61801 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    61861 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    61921 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    61981 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    62041 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    62101 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    62161 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    62221 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    62281 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    62341 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    62401 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    62461 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    62521 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    62581 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    62641 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    62701 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    62761 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    62821 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    62881 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    62941 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    63001 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    63061 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    63121 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    63181 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    63241 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    63301 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    63361 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    63421 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    63481 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    63541 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    63601 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    63661 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    63721 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    63781 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    63841 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    63901 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    63961 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    64021 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    64081 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    64141 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    64201 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    64261 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    64321 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    64381 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    64441 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    64501 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    64561 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    64621 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    64681 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    64741 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    64801 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    64861 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    64921 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    64981 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    65041 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    65101 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    65161 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    65221 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    65281 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    65341 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    65401 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    65461 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    65521 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    65581 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    65641 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    65701 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    65761 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    65821 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    65881 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    65941 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    66001 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    66061 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    66121 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    66181 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    66241 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    66301 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    66361 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    66421 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    66481 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    66541 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    66601 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    66661 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    66721 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    66781 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    66841 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    66901 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    66961 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    67021 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    67081 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    67141 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    67201 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    67261 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    67321 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    67381 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    67441 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    67501 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    67561 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    67621 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    67681 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    67741 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    67801 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    67861 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    67921 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    67981 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    68041 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    68101 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    68161 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    68221 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    68281 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    68341 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    68401 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    68461 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    68521 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    68581 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    68641 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    68701 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    68761 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    68821 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    68881 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    68941 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    69001 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    69061 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    69121 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    69181 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    69241 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    69301 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    69361 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    69421 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    69481 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    69541 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    69601 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    69661 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    69721 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    69781 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    69841 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    69901 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    69961 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    70021 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    70081 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    70141 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    70201 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    70261 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    70321 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    70381 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    70441 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    70501 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    70561 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    70621 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    70681 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    70741 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    70801 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    70861 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    70921 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    70981 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    71041 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    71101 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    71161 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    71221 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    71281 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    71341 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    71401 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    71461 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    71521 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    71581 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    71641 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    71701 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    71761 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    71821 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    71881 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    71941 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    72001 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    72061 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    72121 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    72181 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    72241 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    72301 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    72361 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    72421 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    72481 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    72541 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    72601 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    72661 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    72721 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    72781 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    72841 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac
    72901 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    72961 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    73021 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    73081 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    73141 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    73201 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    73261 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    73321 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    73381 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    73441 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    73501 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    73561 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    73621 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    73681 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    73741 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    73801 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    73861 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    73921 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    73981 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    74041 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    74101 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    74161 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    74221 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    74281 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    74341 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    74401 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    74461 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    74521 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    74581 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    74641 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    74701 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    74761 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    74821 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    74881 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    74941 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    75001 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    75061 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    75121 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    75181 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    75241 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    75301 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    75361 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    75421 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    75481 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    75541 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    75601 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    75661 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    75721 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    75781 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    75841 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    75901 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    75961 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    76021 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    76081 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    76141 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    76201 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    76261 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    76321 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    76381 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    76441 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    76501 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    76561 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    76621 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    76681 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    76741 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    76801 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    76861 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    76921 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    76981 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    77041 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    77101 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    77161 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    77221 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    77281 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    77341 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    77401 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    77461 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    77521 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    77581 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    77641 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    77701 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    77761 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    77821 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    77881 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    77941 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    78001 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    78061 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    78121 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    78181 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    78241 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    78301 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    78361 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    78421 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    78481 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    78541 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    78601 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    78661 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    78721 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    78781 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    78841 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    78901 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    78961 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    79021 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    79081 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    79141 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    79201 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    79261 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    79321 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    79381 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    79441 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    79501 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    79561 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    79621 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    79681 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    79741 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    79801 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    79861 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    79921 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    79981 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    80041 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    80101 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    80161 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    80221 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    80281 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    80341 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    80401 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    80461 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    80521 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    80581 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    80641 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    80701 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    80761 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    80821 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    80881 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    80941 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    81001 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    81061 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    81121 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    81181 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    81241 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    81301 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    81361 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    81421 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    81481 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    81541 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    81601 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    81661 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    81721 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    81781 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    81841 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    81901 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    81961 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    82021 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    82081 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    82141 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    82201 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    82261 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    82321 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    82381 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    82441 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    82501 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    82561 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    82621 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    82681 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    82741 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    82801 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    82861 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    82921 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    82981 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    83041 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    83101 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    83161 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    83221 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    83281 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    83341 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    83401 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    83461 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    83521 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    83581 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    83641 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    83701 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    83761 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    83821 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    83881 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    83941 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    84001 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    84061 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    84121 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    84181 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    84241 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    84301 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    84361 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    84421 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    84481 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    84541 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    84601 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    84661 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    84721 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    84781 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    84841 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    84901 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    84961 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    85021 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    85081 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    85141 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    85201 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    85261 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    85321 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    85381 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    85441 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    85501 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    85561 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    85621 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    85681 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    85741 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    85801 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    85861 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    85921 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    85981 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    86041 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    86101 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    86161 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    86221 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    86281 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    86341 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    86401 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    86461 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    86521 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    86581 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    86641 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    86701 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    86761 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    86821 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    86881 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    86941 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    87001 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    87061 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    87121 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    87181 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    87241 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    87301 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    87361 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    87421 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    87481 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    87541 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    87601 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    87661 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    87721 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    87781 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    87841 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    87901 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    87961 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    88021 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    88081 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    88141 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    88201 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    88261 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    88321 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    88381 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    88441 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    88501 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    88561 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    88621 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    88681 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    88741 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    88801 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    88861 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    88921 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    88981 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    89041 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    89101 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    89161 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    89221 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    89281 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    89341 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    89401 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    89461 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    89521 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    89581 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    89641 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    89701 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    89761 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    89821 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    89881 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    89941 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    90001 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    90061 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    90121 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    90181 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    90241 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    90301 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    90361 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    90421 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    90481 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    90541 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    90601 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    90661 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    90721 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    90781 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    90841 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    90901 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    90961 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    91021 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    91081 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    91141 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    91201 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    91261 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    91321 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    91381 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    91441 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    91501 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    91561 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    91621 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    91681 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    91741 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    91801 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    91861 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    91921 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    91981 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    92041 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    92101 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    92161 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    92221 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    92281 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    92341 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    92401 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    92461 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    92521 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    92581 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    92641 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    92701 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    92761 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    92821 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    92881 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    92941 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    93001 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    93061 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    93121 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    93181 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    93241 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    93301 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    93361 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    93421 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    93481 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    93541 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    93601 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    93661 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    93721 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    93781 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    93841 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    93901 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    93961 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    94021 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    94081 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    94141 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    94201 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    94261 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    94321 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    94381 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    94441 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    94501 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    94561 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    94621 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    94681 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    94741 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    94801 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    94861 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    94921 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    94981 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    95041 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    95101 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    95161 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    95221 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    95281 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    95341 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    95401 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    95461 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    95521 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    95581 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    95641 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    95701 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    95761 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    95821 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    95881 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    95941 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    96001 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    96061 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    96121 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    96181 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    96241 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    96301 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    96361 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    96421 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    96481 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    96541 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    96601 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    96661 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    96721 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    96781 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    96841 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    96901 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    96961 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    97021 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    97081 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    97141 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    97201 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    97261 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    97321 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    97381 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    97441 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    97501 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    97561 gggcaactga tcattgattt ggtgcaaaaa tatattgcag agccattcct aggattatct
    97621 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    97681 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    97741 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    97801 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    97861 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    97921 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    97981 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    98041 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    98101 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    98161 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    98221 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    98281 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    98341 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    98401 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    98461 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    98521 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    98581 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    98641 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    98701 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aagagaagaa
    98761 gcaaaggaga aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt
    98821 tttacgataa agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag
    98881 taactagaaa acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg
    98941 ctcgcataaa aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa
    99001 cgaagaatga attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg
    99061 ttcattacac agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt
    99121 taggaacaat cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg
    99181 aagcaacgta ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc
    99241 tagaaggaat ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt
    99301 aggaaggggc agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga
    99361 aatgtttgat ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg
    99421 ttttcgtaca cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct
    99481 aacaagtcaa gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat
    99541 ggccggcatt ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca
    99601 gatcatggaa aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc
    99661 gccattctcc tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag
    99721 ttatcaacta aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg
    99781 tatggatttt gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa
    99841 ccaacgagct ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg
    99901 caattaccag tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta
    99961 tcagcttgca acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt
   100021 ttgcagcctt gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc
   100081 atgcggctac ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat
   100141 gatcacacct tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc
   100201 atttaaaaag agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg
   100261 gaatgaagga atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga
   100321 acaaacaaaa ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg
   100381 gtatcgtaaa gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga
   100441 agaaacgtta agctcaatct tgccacagca acagccaatt cttaatgact atttttgata
   100501 aaaaacgggt gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa
   100561 ggaaaatgtt aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt
   100621 ttagggtttt cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt
   100681 ttagatgaat ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag
   100741 gacctcccta cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa
   100801 cgttttgcct aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa
   100861 caagctacaa ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct
   100921 cctaagcgat aggtcgctgg ttcaattcca gtcaggaacg tacaaataac ccctgtatcc
   100981 tttgcggtac agggggttat tttgtataag tagacattgt agtttctaaa ctgctatgtc
   101041 ctaaacgttt cgatacgcta agtatattta ctccttgata aagtaaaata gatgcatgcg
   101101 tatgtcttag tccatcaata aacagtcgaa gagaaaatag ggaaaaattg attactaaag
   101161 aaaaacttac taggtcgacg gtgtagttga ttttcatact tcaatagatt cgaattaaac
   101221 gatttcgatt ggcttatacc agtcctatcc aacgaaagat atatcgttct taactctagc
   101281 ggtataactt gaagctttac gtaaagccaa tgggaatgaa ctggagatga ctttttatta
   101341 tttttagtaa tagctgatgg gttaatactt acaactttcc ttttcaaagt accatataaa
   101401 aaaacggtgg ggttgtttag cgaaataaag gatctaagcc aatagcttag atcctttatt
   101461 ctatgcttta tattattttt tgcatgtgtg cgaaagtata ctcaagccca atcgccattt
   101521 cggaaaatcg gtactgtaga gccatcttca cggattccgt cgatatccat cttgtcagat
   101581 cctaccatga aatctacgtg ggtttgactt ctattcaatc cagcttctgc taattcttca
   101641 tcggacattt ctgtaccgcc tttcacactg aaggcataag cagaacctag tgctaagtga
   101701 ttcgatgcgt tttcatcaaa taatgtattg aaaaagatga tgccagattg agaaataggt
   101761 gaagggtcag gtacaagtgc gacttctcct aaacgacgcg caccttcatc tgtatcaagc
   101821 agttttgcta agacatcttc tccttgttcg gcagaaaaat caacaacttt tccatcttta
   101881 aaagtaaatt tcatacccga gatgatggtg ccagcataac tcagtggctt tgtacttgaa
   101941 atgtaaccat ctactcgatg actgtcaggt gctgtgaaca cttcttccgt cggcatattt
   102001 gccatgaatt tttctccacg agcattatag cttccagcgc cttcccaaag atggttttta
   102061 ggcaatccga taatgatatc tgtaccggga gcagtatagt gaagggcaga aaattgttct
   102121 cgattcaatt cttctgcttt ttttgctaat ttttcatcat gcttcttcca tgctaagaca
   102181 gggtcttctt catagacacg agtcgttttg aaaatctgat cccataatgc ctcaacttgt
   102241 ttttcttctg gcagttctgg gaagactttt gctgcccatt gtttaccagc cgcagccacg
   102301 acagtccaac tgactttatt tgcttgagtt gccttgcgta aattcattag tgctttgcca
   102361 ttcgctgatt gataagacgc tactcgatgg ctatctacac cagcaaatgc atcgggatcg
   102421 gcagaaacaa cgctgatcct gcttgctcct ttttcaagcc attcatccgc ttgatcgatt
   102481 ttagattgtg gtacattctc aatacgatcc gttgcagcat gtaaaagaaa ttctctttgg
   102541 atctggtcgt cagtccattg aacgataacc tcagcggctc ctagtttata agcttcttgt
   102601 gtgatcaatc gagcaagggg tgcctgttcc acgctgatct gtaaaacaac tgtgtgtcct
   102661 ttttctgtgg ctacgccagt ttctgcaatt aagcgtgcat atttttttag aagttcatta
   102721 aaatcagata acataataaa aattcctcct caccattttt tctggtaact attagcatga
   102781 taccatttaa ttatgattca aacaatgagg ctttaaataa aaaaaggagg ttattttttt
   102841 aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa gaaaatcaaa
   102901 aactaatttt gcatatcaaa aaaacatgtt acaatgaaat taatcaatgg tggaagcgtt
   102961 ataaaaaaat taaggagatt aaaaaatatg gcaagaaaaa agacgattac tagagaacag
   103021 attttggcag ctgcttatga agtagctgcc accgatggat ttacacgatt tactgcgaga
   103081 aacatcgcga acaaaatgaa ttgttccacg caaccgattt atcttgagtt taaaaatatg
   103141 gatgacttga gacaagctct ttttgagcag atctatgatc atttacaata tgaagtgttc
   103201 actgttgagc atacagggaa tacgattatc gatcttgctt ttaattatat ccattttgca
   103261 agaagagaga agaagttgta tcgttcactg tacttagaag aatatggtgg aggaaaagag
   103321 atgcagaatt tctcttatac ttactttgtg aatacagtga aacgcgatcc agcttatgtg
   103381 aatctttcag aagagcagat cgaatctttg tataatggga catggatcat agctactggg
   103441 atcgcagctt tgatgtcttc aagcatcatc catccaactg atgaacagat tgaaaagatg
   103501 atccaagaca gtatagatgc tatcttgaaa ttcaaagaac caatcgaaat cgattgaata
   103561 tactcaaaag ttccacaaat cagtaggtaa caagagatgt gtcaaaataa ggaaaagttc
   103621 gtttttctta cagcgtatgc atttttaact cccgaagaac tcttccatga atgcaatgaa
   103681 taaaagagcc atacagattg aataaatctg gatggctctt ttattgcaag tatattaaat
   103741 ttttgcgact agttctttag cgaaagcttc taaattttcg atatcttctt cttcagcatt
   103801 cagatccact ttaacatttt cggctccttt tgatgcgcct gtttttgcaa aagcagcatc
   103861 gaaatcatcc accgatttgc agaattcatc gtaaaaagta tcaccagatc cgcagacacc
   103921 gtaaattttt cccaatagat ccaattcttg caagtcttca taaaagtcta cgatctcatc
   103981 aggcagatcg ccatcatcgt atgtataagt agctactacg caaatatctg cctcctcgaa
   104041 ttcatctgca tctacttggg tacattcgtt gatttcgact tcgatatcca gattttcaaa
   104101 tgcttctgca acaatatcgg cgatttcttc agtgtttcct gtcatgctag catatacgat
   104161 ttttgctaaa gccatgatgt gcctcctcgt ttataattat ttaacaatta tgaattatac
   104221 caaaacatca ttgaaaagaa aataaaacct aaagacacct ggggaatgtc atttggctaa
   104281 atgtatgccg ctatgttaaa atgagaggga tttgtaaaaa ggagacgaaa atatgattac
   104341 tttaaaatca gcacgtgaaa ttgaaatgat ggcagaatct ggagctttat tagcagatgt
   104401 acataaaaat ctgcgtgatt ttataaaacc aggaatcaca agttgggata ttgaggtatt
   104461 tgtcagaaac tatatcgaga gtcatggcgg aattgctgcg caaattggct ttgaagggta
   104521 tgaatatgcg acttgtatca gtataaatga tgaaatttgt catggattcc ctcgtaaaaa
   104581 gcctttgaaa aacggtgatt tggtaaaagt cgatatgtgt atcgatttga aaggcggcat
   104641 ttctgattct tgttgggcct atgtagttgg agaatctaca cctgagatcg accgattgat
   104701 ggaagtaacg aagaaagctt tgtacatcgg tattgagcaa gcacaagtag ggaatcggat
   104761 tggtgacatc ggtcatgcta tccaaactta tgtagaaggc gaaaatctag cgatcgttcg
   104821 tgattttatt ggtcatggcg ttggtcctac gattcatgaa gaaccagcag ttcctcatta
   104881 cggagaagct ggaaaagggt tgcggttgaa agaaggcatg gttatcacga tcgaacctat
   104941 ggttaatacc ggaacttgga aaatgaaaat ggatcctaac ggctggactg cgtatacaag
   105001 agacggcgga ctttcttgcc aatatgaaca tacattggct attactaaag atggtccaag
   105061 aattttgaca tcacaaggag aagaaggcac ttattgatct atccagtcaa taggtacggg
   105121 agaggtacat gaaagtttgg aacaaaataa aacaaaatga aaatctgatg cgctttattg
   105181 agacgactca aaagcgcatc actgattcag aaatgagtac gacctctgtc gtcgttgctt
   105241 attatttact cttgtcttta tttccattga ttattgcttt tggtaatatt ttaccgtttc
   105301 tccacatcga tcaagagact gtactgacct atttacgaga agtgattcct gagaccgttt
   105361 accagtttat cggtcctgca atcaaagacc ttttgacaca aagttcagga ggcttgttat
   105421 ccgtttctgc attagcggct ttatggtctg caagtcaaag tatcaatgct ttgcaaattg
   105481 cgatgaataa agcgtatggc gtagaaaata gaaaaaattt tttgatcgtc cgtttcttct
   105541 cactaattgt cattgtcttg ttcatgattg ctattagtgg ggtaactatc gttcttgggt
   105601 tgggacagct gattttggat gctattcagc caatcgtaca aatacccgtg aactttatcg
   105661 atcagttcca aactttgaag tggccaatca cattggtagc tttatttgtc atcatgttct
   105721 tgatttattt aattgtccct aatgctcagt tgaagttgaa gacgatcatt ccgggcacga
   105781 tcttcgctac tgtcggctgg atgctgttat cacaagtgtt tagtatctat gctaggtatt
   105841 ttgcaacgag gatcagcggt tatcagatca taggaagttt tattgtactc atgctttggc
   105901 ttaattttgc tgctacgatc atcattttgg gcggaatcat caatgcagtt gttcaagaat
   105961 acgtgacggg tcatcaaatc aaaaaacgtc gagatccaag ccgaaaatgg ttcaataaat
   106021 tgaaaactaa attttctcat aaaaaaaagt agaaacaaga gttcttattg taaatttata
   106081 tcagtaattt ataataatta tgactcttaa ttaaagtagg tgcccatatg cttgtttctt
   106141 ttagtatggt tataagattt tttctgacaa ttttattatc actgatatta acacctgtat
   106201 tcaaaatcat ttcagtacaa acaggtatgg tcgacaaacc aaatgaaaga agaataaata
   106261 aagttccaat gccttcagcg ggcggactgc ctatctttat ttcatttgtt atatcgactt
   106321 tattcttatt ccgtaaaatt atccctcaat cgtatattgt accgatcttg attgcttctt
   106381 tagtaatcat agtcacagga gtgctcgatg ataaatatga gctgacacct aaacaaaaaa
   106441 gtatagggat cttgattgct tctcttatta tttattttgt agcagatata cgtattgatt
   106501 ccttcacgct tccttttatt ggatatatcc aactaggctg gcttagtttt cccgtaacga
   106561 ttttttggat cttcggtatc acgaatgcgg tcaatctgat tgacggtttg gatgggctgg
   106621 cggcagggat ttcattgatc ggtttaatta cgataggaat cattggatat tttttcctcc
   106681 atgcttctac agtgtatatt ccagttgtta ttttctgtct tgtagcaagt attatcggtt
   106741 tttttcctta caatttttat ccggcaaaga tttatttagg cgatacagga gctctattcc
   106801 tagggtttat gatggccgtc ctttcactac aagggctgaa gaacgtgact tttgtttcat
   106861 cgatttcttt gctgatcgtc atgggagtgc cggtaacaga cacgtttttt gctatcatta
   106921 ggagaaaagc aaaccgtgtt tctttttcta cagcagataa aaaacatctt catcaccgac
   106981 taatcagttt aggctttact cataaaggtg ctgtcctgac gatttatgcg atggctttga
   107041 tgttttcttt cacagctatg gtcatgaatt atactggaag aatcggaacg atcgtattga
   107101 ttattgccat gttgtttgct gcaattttat tatttgaatt gattggtctg atcaatgaga
   107161 aacatcagtt tattttaaaa acattgcgat ttttagggaa taaagagtac cgtacgcaag
   107221 taaaacagaa atatttgaag aaatggaacc gtcatataga tgaataacaa attaaaccag
   107281 ccgatcctta ttagtattgt gacggcaaac agtgataaaa tttttcggac actagataat
   107341 tttatttcaa ctgtaaaaga tgaaacagca gtaaaaatcc gaatttttga caacaattca
   107401 cttcccgagt acaaaaaaag actaacagag tataggaaat atccctacat cgatatctat
   107461 tttcatgatg aaaattccgg atttggttat ggacataatc acaatctgat agctagcgat
   107521 tctcgctatg gcattatttg taacccagat attttagtgg atgaagagac agttacagca
   107581 ttggtcttgt tattgaaaga acatccggaa tgtgcgatgt tagcacctaa gattcttaat
   107641 gaagatggaa caactcagca cctcatccgt gagcagctgg ctgtttttga ttacgttttg
   107701 cgctttgtgc ctttcaaatg ggtcaaaaaa aagtttgaca aacgactggc tgtgtttgaa
   107761 tgccgaggat tgcctactga tgctaatagt tatgtacgga tgatctcagg tagttttatg
   107821 gtagctgata ttgataaatt taaagagata aaggggtttg atgagcgttt cttcatgtac
   107881 tttgaagaca acgacttgtg tttgagtttt gaaaaagcag gttacaagtt attgtacaca
   107941 cccttttatg cagttatcca tttttacggg aagggtgcgc atcgaaatgc aaaactgttt
   108001 cgcgtgtttt tgacttctat gatgaaattt ttcaataagt gggggtggcg ttttttctga
   108061 tgaaaccaga aatattagta gtcgttgtgc tttatcagca actattcagt caatctccag
   108121 cttatggacc attaagcaaa gcacttagcg aaaagcaaat tcagcttatt atttacgata
   108181 atagtcctca aaggcagcaa aatgagctgt ttgaaaaaga aaatatcgtt tatcatcatg
   108241 atccgcaaaa tcctggttta gcggtggctt ataattttgc gttgtctcaa gcagatgaag
   108301 gaacacgttg tctcgtcact ctggatcagg atacgaggct agtcgacgat tattttgaac
   108361 tattgcgtaa agtaaccttt acagatgaat gtgttgctgc agttccgatg attttttctg
   108421 ggagccgaca agtttctccc ctatattcag atcgttatat caatcgtaca gctcaagcag
   108481 tagaagttga aggcccaaca gctgaacgaa tcatggcaat caattcaggt gttgcctggt
   108541 ctgtcaaatt tctaaaaaag attggcgggt ttaatcctgc tttttcttta gattttttgg
   108601 atcattggct gttttggaaa gtggatcagt tggaaaaaac tgtggagatc cttcctgtac
   108661 gaatggaaca tgatctttcg gttttagact atgaaaaagt ttcaagtaat cgataccgtt
   108721 ctattttaca agcagaaaat cgattttatc atgaatatga tcaaaggcat ttatccagtc
   108781 accgacgaca attattgtta agaacagcta aacagttctt gaccgtcaaa aaccggcata
   108841 tctggcggat gactttgcgc tcatatgtta agaattggaa ggtgtgaatt cgtgcgttcg
   108901 gtttgtatag ctacctttaa tggaggcaga tatttaagag agcagttaga tagtatttta
   108961 tctcagcttt cagcagaaga tgaagtagtc atttctgatg atgggtcagt agatgataca
   109021 tgggatatat taactgaata cgcagaaaaa gataagcgaa tccagctttt ttcggggcct
   109081 cgtcaaggat tgatcgctaa ttttggttat gcgatccagc agacgaaagg agatctaatc
   109141 tttttggcag accaagatga tgtttggttg gagacaaaag tgaaaaagat cactgactat
   109201 tttgagcaga atccggatca gctagtagta gtttctgatt taatcattgt ggacagcgag
   109261 ctgcaagaga tccatgattc gtatttttcg tttcgaaatt ccaaagaagg atggcttaat
   109321 aatgtgataa gaagtcacta tattggtgct ggtatggctt tccggtcttc tttaaaaccc
   109381 gatattttgc cgatcccgtc agaagtgccg atgcatgata tgtggattgg tttgctggga
   109441 ggaaagaaga ctgggttcct aagagaaccg ttaactctgt accgaagaca tgatttaaac
   109501 gcgagtgaga ttgaaacaca ttctaaatta cggcaaaaaa tttcttggcg tgttcatctg
   109561 attacggcgt tgatcaaacg aaaatggttt aaacgttgat aaaagatgtt taaatcctta
   109621 atatttccat gacaaatata acaattgatg agataataac aaagtgaaat ttgaaatcaa
   109681 aaaggagagc agatatccat gaagggaatc attcttgctg ggggcagcgg aacacgtctg
   109741 taccctttaa caaaggcaac atcgaaacaa ttgatgccga tttatgacaa accaatgatt
   109801 tattatccaa tgtctactct gatgttggcg ggaatcaaag aaattctgat tatttcaacg
   109861 ccacaagata caccacgttt caaggaatta tttggaaacg gaaatgattt aggactgcag
   109921 atcgaatatg cagtacaaga aagtccagat ggattggcac aagcgtttat cattggagaa
   109981 gaatttattg gagatgatag tgtctgcttg gtattagggg acaatattta ctatggtggc
   110041 ggtctgtcta aaatgttgca acgagcagct tcaaaagata caggcgcgac agtatttggc
   110101 tatcatgtca atgatccaga acgctttggt gtagtagaat ttgatgagaa tatgcaagct
   110161 ttgtcgattg aagaaaagcc tgctcagcca aaatcaaatt acgctgtaac tggcctttat
   110221 ttttatgaca atgaggtaat ttctatcgct aaaggtatca aaccttcaga acgtggagaa
   110281 ctagagatta cggatgtcaa taaagcatat ttggataaag gaaaactatc cgtagaactt
   110341 atgggacgcg gatttgcttg gcttgataca ggaacacatg aatcattgct tgaagcatct
   110401 acctttatcg aaacaattga aaaacgtcag aacttaaaag tggcttgttt ggaagaaatt
   110461 gcttaccgta tgggctacat cgataaggaa caattgctgg cccttgcaca gccattgaaa
   110521 aaaaatcaat acggtcaata tttattgaat ttggccgctg aataaggagt ccttacagat
   110581 gaaagtaaca gatacaaaat taacagatgt caaaatcgta gaaatggacg tctttggcga
   110641 tcatcgcggt ttcttcacag aaagttattc aaaagcaaaa tttgcagaac atggattgaa
   110701 atatgacttt gttcaagaca accattcact atcagctgaa gctggtgtcc ttcgggggct
   110761 tcatttccaa aaaggagaag ctgcacagac aaaattgatt cgggtagtca ctggagcagt
   110821 tttagatgta gtagtggata tccgcaaagg tagcccgact tatggtcaat gggacggatt
   110881 tatcttatct gaacataatc atcgacagtt gcttgtacct aaaggatatg cccatggatt
   110941 cgttactttg acaccaaatg tgaacttcct ttataagtgt gataattact ataatgcaga
   111001 agcagatggg ggcatcgcat ttgatgatcc agatttagcg attgattggc caattgatcc
   111061 atcaagagcg attatgtcgg aaaaagatca ccgtcatccg acattaaaag aatttgaagc
   111121 agaaaatcca tttgtttacg gagaaatcta agaattaagg agaaattttc ttatgaaaaa
   111181 catcattgtt accggaggtg ccggattcat cggctctaat ttcgttcatt acgtggtaaa
   111241 caatcatcca gaagtgcatg tcactgtttt ggataaactg acttatgcag gaaacaaaga
   111301 gaacttggcc ggacttccta gcgatcgcgt agaattggtc gtaggagatg ttgcagatgc
   111361 agaattagtg gaccgtctag taaaagaagc ggacgcagtt gttcattacg cggcagaatc
   111421 ccataacgat aattcattaa aagatccttt cccatttgta caaacgaata tcattggtac
   111481 gtatacactt ttagaagctt gccgcaagta tgatgtacgc tatcatcacg tatctactga
   111541 cgaggtttac ggcgatcttc cattacgtga agacctgcca ggacatgggg aaggcgaagg
   111601 agaaaaattc actgcggaaa caccatataa tccttcaagt ccatattctt ctacaaaagc
   111661 tggttcagat ctgttagtaa aagcatgggt gcgttctttt ggcttacgtg caaccatttc
   111721 aaactgttca aacaactatg gtccttatca gcatattgaa aaatttattc ctcgccaagt
   111781 aacgagtatc ttaagcggta ttcgtccaaa attatatggc gaaggaaaga atgttcgtga
   111841 ctggatccat acgaatgatc attcttcagc agtgtggctg attttgacga aaggcagaat
   111901 tggcgaaacc tatctgatcg gtgcagacgg tgaagaagat aataagaccg tgatggaatt
   111961 gatcttagaa atgatgggcc aaccaaaaga cgcatacgac catgtaaacg accgcgcagg
   112021 gcacgatttg cgttatgcaa tcgattcgac aaaattgcga gaagagctag gttggcagcc
   112081 tgagtttacg aacttccgtg atgggttggc tgacacgatc aaatggtacg aagaacatga
   112141 agactggtgg aaaaaagaaa aagaggccgt tgaagcagca tatgccaaaa atggacaata
   112201 atacagagga aatttcactg tattaatgga aaaacacatg tgaggcaggc gctcgcctgt
   112261 ctctttttta aaataaaaat caaggagcgg ataaccatat gtttttatta actggaggaa
   112321 atggacaatt aggtacagaa ttgcgccatt tattagatga aaaaaatctg ccatatgttt
   112381 caacagatgc aaaagacttg gatatcacag atgctgagaa aacaatggct tatattacag
   112441 aattaaaacc cgatgttatt ttccattgcg cagcttacac agcagtagat aaagcagaag
   112501 aagaagcaaa agaattagat gagaaaatca atgtcgatgg cacaagaaat gtagcacttg
   112561 cagcaaaagc tgtgaatgca caattcgtct atatcagtac agactatgtt ttcgatggaa
   112621 aaaacaaaaa tgtagaatac gaagtagacg accaaacgaa tccactaaat gaatatggtc
   112681 gtacgaaatt attaggcgaa aaagcagtag aagagatttt agaagactat tatatcatac
   112741 gaacttcttg ggttttcggg atctatggac acaatttcgt cttcacgatg caacgcctag
   112801 cggaaacccg cgacaaactg actgtagtaa atgaccagtt tgggcgacca acttggacac
   112861 gtactttggc agaatttatg gtatatgtca tcgaagaaaa agcaccattt ggtatttatc
   112921 atttgtcgaa cgaaaatagc tgttcttggt atgagttcgc aaaagaaata ttgaaagata
   112981 cagatgtaga agttgcacct gttacgtcag aagaatttcc gcaaaaagca acacgtccgc
   113041 agtattcagt catgagcctg aaaaagacag aagcgttagg ttttgtcata ccaacttggc
   113101 aagaagcact tgcgcaaatg ctggaagctg ccaaaaaaat gaataagtaa tttccttttt
   113161 atcaaataga atttggagca tgatatggaa aaacaaacaa gaagtgcacg acgaaaagaa
   113221 aagccaaaag cgaatcaggc agattcgaat ttggtgcttg acctgtttct aaaactccgc
   113281 tatcttattg gcgttttagt catcgtggtc atcgttactt ttaatttaaa cggaagttca
   113341 cttggtgttt gggataaata tgtgtcgcaa agagacgatg ggaaaaaaac tgatgtgatt
   113401 tttggacaga atcgggaagt gcggtcagac gaatggctgg tgcagacacc tttttacctc
   113461 tcacaagctg aagaggactt ttcgttggtg aacgaggatt attcattaaa cggacaaaac
   113521 atgatcattg cgtataattc tcctgtaaag gatattacgg ttatcggaaa accattcaac
   113581 tggggattct ttttcctcgg aagagatcga ggactttcct tttattgggc gatgaaatta
   113641 gtcgtcatgg tcttgcttgg attcgaagta ctgatgatat taacgaagag gaaaaaagct
   113701 ctatcgctaa tcggtgcatt tgcgctgaca ttttctccag cagtccagtg gtggttcatg
   113761 caacatgtgg gcgatttgat tttctttact ttgggactga tgattgcttt ttatcattat
   113821 ttctacaatc atgataaaaa atggctacga acgttgatga tgcttttggt ggttattttc
   113881 ggtattgggt ttattttagt gatttaccca gcccatcaag tgatgctggc gtacttgctg
   113941 attttttatt ttgttggcct gcttatttac tatagcaaga aagtttcatg ggatctgttg
   114001 gatgtagtgt tgattggagg ggcagtttta tttattggcg gagtcatggt tcatttctgg
   114061 ctaacgtcaa aagacgcatt gttagcttct ttgaatacct tatatccagg aaaacgggta
   114121 agtactggag gaaagtggac gattggcgaa tttttttctt tccttacaaa ttggaaaatt
   114181 ccttttgaag atatcaacta ttcgaataac tctgaagtgg ccctgttcta tcactttttt
   114241 ccaactgtat tcctagcaag tccatttgtg ttatttggca aaaaagatag cgaacaaaag
   114301 ttactgggac gtattttgat gattttttgt ctgttttcta tcttttggat taccgttggg
   114361 ttgccaaaat ggctagcaga aatcactctg ctgtcttatg ttcctccatt aagagccatc
   114421 cttactttta gttttgcggc ttgtttatta tcgatctggt ttatcgctta tatttggaga
   114481 gaagatgtta ttcctatttg gtataaattc attttgttgg cagtaaatgc atggtcttat
   114541 ttctatgcga ttacgcattc gaaaatggat aattattttt cggattttga aaccatcgca
   114601 gttgctgtgt tgggaacagt gattttagct tttgcattat ttggctggag acgcttgttt
   114661 tacctcgctt ttgtcgcctt agttatcgta tctggctttt tcgtcaatcc agtcgtagaa
   114721 ggtacaggag cgatttttga aaaaacactg gcaaaagaaa tccaaaaaat cgatcaacaa
   114781 gatcctggta aagtttggtt gtctgaagat gaattatata acttcacccc aacattggga
   114841 gtgaaatcct tcaatacagt acgcttttat ccagatatag ctgcatggaa gaaaatcgat
   114901 ccgaaagaag aatacgagaa gtattataat cggtatgctc atacaagagc ttttatcgct
   114961 aaggaagata caaagttcat tttagatcgt ccagatatgt tttcggtcac gttgaatttt
   115021 gaagacgcag aaaaattagg gatcgactat gtgatcagta aacgaccatt aactgattac
   115081 aaccaattat atgaagcgca gttcacgcag ctatatggac cggataaaga cggttatatg
   115141 atttttaaag tagaatatcc ggattatcca gatgtaccac aaacacaaac tcaggaaaat
   115201 cagctagtcg tacagtaata ggctagctga gaataatcct gaaatattag tagaaaggag
   115261 agtgaagata attcaagcaa tcgtacaaaa atttgtcaac aaaatgagtg tcggtttctt
   115321 gcttttgttg ctgacgctag tcttttttgt ttggacagca ggggtaggtt ttccagcaac
   115381 tttgtttata tataacaata gttcattact tatcgctttt agtgtggcta ttttgctcat
   115441 tttaaatgtt tataaagcaa aaggcgtcga ttttctttat ctaggtgttg cgcttgccct
   115501 tttcattttc ttttcttact ttcgggaatt aagaaatgag tctttatttg gagacttact
   115561 gtttcctttg gttattttat tttgtttagt aattaaatgg atcgagtgga caaaatggga
   115621 tcgcggtatt tatctgattg tctttacggt cttcctcgga atcacgttat atcgtgtatt
   115681 tacagagatc caagtaccag aaggacaaag catctgggct ccaggcaatt cgctttctga
   115741 tatttggatc aatacgaata caatcggaag ttctttgatg actcttgctt tattgatcag
   115801 tggatttgcc agttcttttg aacgatggta tgttcgaatc atcggtggga tagcagtcgt
   115861 tgcagccttt ttaggtattt gggtctgcca atctagaggt gcattagtcg cgttgattgt
   115921 ttttgcgata ttggatcttt tacctaaatc gttgatgcgt gtcattcgtg cgccatttat
   115981 tgcctacacg gttacgattc ttttagcttt gccgatttct tatctggcgg ctgtctctga
   116041 aaaagtcaat ttgtttaccg atcgagaaga tatttggcat aaattctatc agacgctagg
   116101 agaaaaatct gaacaagttt tactaggaat gaaaacattt atcttccagc gaggaaacca
   116161 atttttagga aaccacaata gttataattc tattttgaat atctatggat tgataggatt
   116221 tgggattgct gctttgctgc ttatcctttt tattggtcgc ttaacgttga aagcagattt
   116281 atccaacggg cagatgactt ttatctgggc cttttttgcg gtgatgatgc agtcatttat
   116341 ggaggataca ttgacttcat acccatgggt tccgattatt tatatcctgt tggctatggc
   116401 cgttcatcga tatgatgaac cagaagttgc ggaggaattc tatgttatgg aagaggcata
   116461 tgacgatatg acttatgatg atacatctta tgaggaacaa ccagatagcg ctcattcttc
   116521 caggatccaa cgctatcatt gataggcaca aaatacgaga gaggctgtga caaccgtcgc
   116581 agcctttttt ggtttattgc aattgtctag catcttctgt aaaatagaaa cgttagagaa
   116641 aatcttgtat catagaaaat taagaaagaa ataagaacgc agctcggtac caaaaatatg
   116701 gtaaaatggc acttgactgt aattcactga aaatcgcttg gaggaacaca aatgtttaaa
   116761 gatttgtatt tatttatgaa ggacatttat cagaacagaa aattattgct acagttttcg
   116821 atcaatgact tcaaatcaag atatgcagga tcgtttttag gaatcttatg ggcgtttatc
   116881 aatccgctat ttactgtatt gatttattgg ctggtatttg gtgttggact gaaagcacaa
   116941 atgatcgatg caaaataccc atttatcgta tatttagtta ccgggatgat tccctggttc
   117001 ttcttctcag atgcatttgc atcaactacg gttgtttttc gggaatatac ttatttagta
   117061 aagaaagtcg tattcaatat tcgtatcttg cccacgacga aaatcctatc caatctgtac
   117121 acccatttat tcttcattct gattggcata gctgtcgcgc ttataaatgg tatatatcct
   117181 tcagcaatgt ctttacagct gatttattat ttattttgca tgtgtgcttt cttgacagga
   117241 ttgacttggc tgacggcatc gatccaacca tttttacctg atatcatgca attaattacg
   117301 atcgtgctgc agttgatcat gtggacatta ccgatattat ggagccctaa tcagtttgat
   117361 tcaggtcttg taagcatatt gaaattaaat ccgctgtact atgttgttca aggttatcgt
   117421 gagtcatttt taagtgaagc ttggttctgg gagcatggaa catatacttt gtatttctgg
   117481 gttttcacgt tagtaatgtt cgtaatcggc tctgcagtgt tccgtagatt gaaaccacat
   117541 ttttcagatg tgctgtaagg agaatagcta tgacagaata tgcaattaga ctaaaagata
   117601 tcacaaaatc ttataatatg tacgcaaaac cttcggatcg atttcgtgaa gccctcaatc
   117661 cttttaagaa atcctatcat gatgtatttt atgcgttgaa aaacgtgaat gtagaaataa
   117721 aaaaaggtga gatgatcggt ttcgtgggag aaaacggatc gggaaaatct acaatgttga
   117781 aaatcattac gggagtactg acaccaacat ccggcacgat ggaaatcgaa ggaaaaatct
   117841 cagctttgct agaactagga tcaggattca atcctgagta ttctggctat gaaaatatct
   117901 atttaaatgg aatggttcta ggattttcaa gagaagaagt cgatgaaatg gtcgatgata
   117961 tcattgaatt tgctgacatc ggagaccata tttatcaacc tgtaaaaact tattcaagcg
   118021 ggatgttcgt tcgtctagct tttgctgtag caatcaatgt gaatcccgat attttgattg
   118081 tggatgaagc attggcagta ggcgatcttg aatttcaact aaaatgtatg gaaaaattta
   118141 cagaaatcaa gaactcggga aagacgattt tattcgtttc tcatgatatc agttcgattc
   118201 gtcgtttttg tgatcgttcc tattggttga aaaacggaga agtcgtcgaa tatggcgata
   118261 cgatggaagt aacgacgaat tatgaaaact ttttaaagaa aaaatcagtt aaaacagtgg
   118321 atcggaataa aaaccaagta gaacggttcg ccgctccaga tatcgtcgac gtgacgaaag
   118381 cggaattgtt ggataaagac aaaaatccgg tggatatgct ggaacaagga gaaaaactat
   118441 acgttaaagt aacttatgaa gtaaaagatg atacaatcaa aaatccggtt ttaggaattg
   118501 cgctacgaac agtcgacaat ttttatgtat gcggacttaa tactctacta gatggtatca
   118561 agattccttg gaaaaaagga gagaatatct tttatctgga gtatgaaaag ttaggtttgt
   118621 tagctggtga atattatttt gacgttgcgg tctttgaaga aaatgcaacg gttcctttgg
   118681 tatataagac aaagtacatg aacttgtttg tcagcggttc ttatatcgga gaaggcatcg
   118741 tggtcttaga ccataaatgg gaagaaggga ctcatagcaa tgaaatatga ttttgaaatg
   118801 gacctagatg aacaaagcag tgtgggaaaa attgccgcac agatcaaacc aggaagtaaa
   118861 gttttagagt ttggtcccgg aaacggccga ttaacgaaac atttaatcgg agcaaaacaa
   118921 tgtgaagtga gcattgttga gttagataag gaattgttcg attttgtcag cgaatttgct
   118981 caagatggat tttatggtga tatcgaaagt tttgagtggg caaactatta tgctggacag
   119041 acatttgatt acgtcttgtt tgcggatgtt ttagaacatt tggtagatcc tggaaaaaca
   119101 ttgaagaaag tcagagaatt tttgaatgaa gaaggagaga tcctgattac attccctaac
   119161 ttggcgcata attctgtcat gatcgatttg ttcaataacc aattgccgtg ggcttcttat
   119221 ggtctcctag atgaaaccca taattctttt tatactcatg atggcttcca aaaagttttt
   119281 gaaaaagcgg gattattcat taatattgaa gattatttgt acttagctgt aggtgataca
   119341 gaattaaaat caacttatga agaattgccg gaagctgtac gctatgattt caaaatgcga
   119401 ccatttggtg aagtctacca atactttttc tcattgatga aacatccagt agcacaaagt
   119461 tccattgcag agccacaaaa ttctaattat gtaaaagtgt tagaagtaac ccagcaaacg
   119521 aaacaagatg aaacaatcca acaaatacca ttcaataatt tcacagggga aaatgagaca
   119581 ctaagtttcc ccgtatcaga aacaacagaa agaatggtgt ttcgcttcgc gcaacaacct
   119641 agctttatcg aattcagcgc agaagctgcc ggagaaaaac taacatttat cgattcgaat
   119701 gctgtagtca aaacagtaaa tgattgttat ttgtttgatg ggaaagaatt accagagttc
   119761 gttctaacgg atatttctgg gaaagaagtg actatccatt gtcactatcg ctttatcggg
   119821 gaattgacac caacaatgaa agaattgttg gaaacgattc gtcccatggc tgaagtcaca
   119881 aagcagcttt ctgagaaaaa tgacgaatta gaaagagaaa atcatcatct tcttgaggaa
   119941 aatacaagac tagaccatac gttaaaaaca accacaaatc gctattgcac attgttggaa
   120001 agtgatgaat ggacaataaa acatagacta ggacgacgta agaaagaaac ttcgaaaaaa
   120061 atccaagaaa aagaactttc tatatgtatt gacgagaaaa tctgggatgc agaaacaaaa
   120121 atattaaaaa tcatcggatg gggaatcgct aattcggatc gccagccctt gtcctataag
   120181 ttatcagctg accaatcgcc atttttcgaa gcaattcctg tttcacgaga agaagtcaat
   120241 caagcagaac agctcgcaaa aggaacagaa gcgggatttg agttacgaat cctttgcgag
   120301 caagaacgtt cattcttagt ggaagcagta gctcaaaatg gccaatcatg gatcattgaa
   120361 atgtaaataa tgattaactt ataaagataa ggagtaaacg aatcgtgcaa aaaaaagaaa
   120421 ttgccgtatt tatcgatcaa atcactcgtg aacgggccac tggcgatttg cttattgtgg
   120481 gctgggcaat tgatgaagtg accaaagaaa tccctacgat aaaggtagaa aaagaaaatg
   120541 tgattgctga agcaacacat gtcgtaagat tggatatcaa tcatttatat aatcttgatg
   120601 ttaagaccca aagcggtttc aaggtacgtt tatcagggaa aatgagagga aaagccatct
   120661 tagacttcca aacagcgaga catcaaaatg gcatagctgt gaaattaaac ggtaaatacc
   120721 cttatgatga cggcattgaa tcttcatggg aaagaaaaaa aagattactg aaaaaaggga
   120781 taaattatgc aaggactcat ggcgtaaaaa aagcgatccg tcgggtcaaa ttggaattga
   120841 agccaggatc gatcgactat gcagagtgga tcagtcgtca tgagaaacca gatttgaaag
   120901 agcaaaggaa agaagcacaa ggatttgcgt accgtccatt gatttccata cttatgcctg
   120961 tctataacgt tgaaatcaaa tggctcgaaa aatgcattga ttcggtactg gatcaaacgt
   121021 atgatcattg ggaactatgt atcagtgatg atgcttctac tgatccagct atccgaaagt
   121081 gtctggaatc ctatcaagca aaagatgata gaatcaaagt cgttttccga aaagaaaatg
   121141 gacacatcag tctggcaact aattctgcat tggaaatagc agaaggagaa tttatagcac
   121201 tcttagataa tgatgatgaa ttaccgccat ttgcattata tgaagtggca aaagtgctaa
   121261 acgtacatcc agaactggat gtgatctaca gtgacgaaga taaaatcgat gcagatggca
   121321 atcgctttga tccgcatttc aaagcagact ggtcgcctga tacattgatg ggaaataatt
   121381 atatctctca cttaggcgta tatcgaacaa gcattgttaa agaacttggc ggtttccgta
   121441 aaggctatga aggttctcaa gattatgatt tggtccttcg tgtgacagaa caaatccctg
   121501 cagatcatat ttaccacatt gaccgagtct tgtatcattg gcggacgatc ccaggatcaa
   121561 ctgcaagtaa tggcgaagcc aaaagttata tctatgattc cggagtgaaa gcattgacag
   121621 atgccctgtc tagaagaaac atcaaaggaa gcgtacgtcc tggcagaatt tctggatttt
   121681 atgagattgc ttatgacgtc ttgcaagagg atttggtcag tgtgatcatt ccaacgaaga
   121741 atggctatga agatctaaaa acttgtgtgg attcgatcat cgaaaaaacg tcttattcaa
   121801 attatgaaat cattattgcg gataacggaa gtacagatcc aaaaatgcaa gaattatttg
   121861 cagaatacaa gcatcagctg aaagaccgct ttatcgtaga attgattgac attccattca
   121921 attattctcg aattaacaat ttagcagcag aaaaagcaaa cggcaaatac tttttattct
   121981 tgaataacga tacagaagtg attgagccag attggatgac ggtgatggtt tcttatgcac
   122041 agtttgatcg aatcggctgt gtaggagcaa aactgtttta tccagatgat acaacgcagc
   122101 atgcaggagt attattagga atcggtggtg ttgcagggca tgcattgaat aactatgatc
   122161 gaacacattg tggttatttc ggccgattgg tcattgacgt caattactta gcagtcactg
   122221 cagcttgtat gatggtcaag gcagctgact tcaatgctgt taacgggttt gatgaaacat
   122281 tagaagtagc attcaacgat gttgacttgt gcttgaaagt ttatgaactg ggtcgttaca
   122341 atgtctatgc acatcaagca gaattgtacc atttcgaatc taaatcaaga ggttatgaag
   122401 atactccaga aaaacaaaga cgttttgctg gtgaaatcaa aaaaatgcaa gataaatggc
   122461 cagcatatat tgcgcatgat ccattttaca atgataattt aacaaaagac ggaatcggcg
   122521 acttttcgtt gagaccagat taaactataa taaaaagctg ttatgttgtg attcgctcgc
   122581 acataacggc tttttatagt tttttgtcaa aaaaaggcag aaggtgccga ctcaaaaaaa
   122641 taagtcgagg ttgggacaga agtgtttaac tccgagaaat aagaagaaat tcacgaaaat
   122701 tgcttttcaa atttttgtga atttcggctt atttaagaag aagttgcttc tgctcccacc
   122761 gtttattcgt ttttaaagaa tgagacaaaa gtgtttctta cttttgtccc acgctcttat
   122821 tcatgctcag agcgccaata gatacaaaat catagccaaa gtttcacgaa ccctagctgt
   122881 caaagaatcg atattttatt ttaatatagg ctattttaaa ataaatggtt taaggtattc
   122941 ctctacaaaa gccactctat caattatcat gggtttaaaa taagaagtga tggagataac
   123001 cagattgttc ttagcgtcaa tataaagaac atttccacta ttaccaattg ctgaatatat
   123061 ttcgttctca gaatctaata cccaccataa atagccatat ctcatatttt gtaagtcttc
   123121 taaagcactg gttctaggtt tgaacatctc gtttatccac atgggtggta cgatttgttt
   123181 tcctttatac atccctttat ttaggcataa tacaccaatt tttgccagat cacttgccga
   123241 taaacacaaa ccatatcctg ccgtcccgat atttagtgga tcacaaaacc agacattggt
   123301 tttaggagac ttatccattg taaactgctt atgttcttca gctgtttcag cataataatt
   123361 gacatggttt ggtatcccta taggatcaaa tagatactta ttggcaaaat caacagttgt
   123421 tagcttactt gtctgataca acaagccagt taatatgtgt aaacagacag tttgatagtt
   123481 gaattcgccg gttatacctt ttcttcctcc caataaatct agtgatgttt ttgtccaatc
   123541 ttcacttgaa cagacttttg tccatggatc gcctttacat ttgtagggtg ctttcattgt
   123601 tagcaagtgc ttgattgtaa tagcgtaaat agttttctcc ccgcgtttca ctttatactc
   123661 tggaaaataa tctaatactt tatcatcgat acttttgatt aatccttgct ctaaagcaat
   123721 gccaatcagc aaagatacaa tacttttcgt tgctgacatg atatgacaag tatcgtcttt
   123781 tttataacca ttccattcat ctgtataaac ttctacccca tccttatatg cgacaatttg
   123841 acatatattt ggttgtcgaa aaccaataaa atcatgcaac tcttgtttat tcataagata
   123901 ttacctcact ttcttctata agcataatgt cattaaaaat cgatttcaat tttttcgatt
   123961 cttcacgtaa tacaggtatt cttatggact agacagcgct ttgggaagat ggattctctg
   124021 aaaaatataa aggttcactt tatgagtgga taaaaaaagc agcaaatact tcgacttctt
   124081 taatttacca acattagtga aaatcgattc atctggagat ttgtaattta gagtccttgt
   124141 cttgattaga ggtgtataaa gctttttatt agacattttt aaattccatt acaatttgct
   124201 tataaactag gtttttaaag gtgactatgg taagataata gagatattat tttgtgatta
   124261 ggagtgtata tatgaataaa aatggagaat ggagtgctgc acgacggata ttcattatat
   124321 taatggatgt tattgtatac aatgcagcaa tatatagtag tttcttactg aagtttcgtg
   124381 gagagatccc attacgaaat ttagaaactt tccaacactc agcaattatt atttcagtga
   124441 tttttattgc gttgaacatt ttgttgggtg catatgtttt ttataatcga atgatcagtg
   124501 acatcgtgtt tgtcacagtc atcggtcagg tattgatgac actagggatc atggtagtga
   124561 cttttgcggg ccgctggttt gcttttccac gtcttgttat cttactttca tttgttctcg
   124621 gtacgctttt cttaagtatc tatcgtattc tcgtatataa actttattta cgcgtaagtc
   124681 gagataaaaa ggtcgtcatt attgggttag aaaaggatgt cgcaccagca atccaaaatt
   124741 ttggttcaaa gaaaaacaac aaacataaag tcgaagcagt cattcttgat aactattatg
   124801 caaacatcaa aaatatgatt aaagacatcg atattgtcta tcttgcttct catatcgaag
   124861 aaactgaaaa aatcaaaatc tatcaattag ttactaaaaa tgaaaaaaaa ttgtttttga
   124921 atacaacatt tgaaaacttg acattgatca atccaaatat tatgaatttc gaagatgaaa
   124981 gtattattga agcatctgga tttagaatcc aacctgaata tgaactattc aaacgtttgt
   125041 ttgatatcat cgtttcactt gttttattag ttattgcttc accatttatg ttattcacag
   125101 caattttagt caaagtgaca tcaccgggac caatcatcta taaacaagtg cggatcacaa
   125161 aaaatcaaaa ggaattttca atctataaat tccgctcgat gaccgccaca gccgaagcaa
   125221 aatctggacc agttttagct aagagcaatg atgcacgagt aacgcctgta ggtaaattta
   125281 ttcgagctgt ccgctttgat gaattaccac aaatcttcaa tgtgctgaaa ggcgatatgt
   125341 cgatcgtagg accacgtcct gaacgttctt tcttcgtcga tcagttcaat gcagaaaacc
   125401 cttattacta cttacggcat aatgttcgtg ctggtatcac tgggtatgca caagtctatg
   125461 gaaaatatgt ctctgattac aacagtaaat tacgttttga tttactttat atcaaaaaat
   125521 attcattgat gcttgattta aagatcttgc tgcaaaccat caagatccta tttgataaaa
   125581 tgtcctctca aggacttgaa gaagacgaaa atgtgatctc attggcagaa attgcttttc
   125641 cgaatgaaaa aatctttcgc tagttgggag gcgtaaaaat gaacaatccg aaattcagca
   125701 taatcgtacc agtatacaat gcagaagcaa ctattggtcg aacgattgat attctaaaag
   125761 aattggatta tgaagagtac gaagtcgttt tagtaaatga tggatcaaca gatcaaacac
   125821 aggaaatcat tcaagaactg attgaaaatg atgaacgttt tgaattgatt actacgacca
   125881 atcaagggcc cggattagca agaaacgaag gaataagaca gtcaaaagga gaatatctct
   125941 tattcttcga tgcagatgac acgccagtaa aaacgatctt gtcagattat gatcatttgc
   126001 tagaaaagag tccaaaagcg gatcttgttg ttagttcgtt tgtttttcgt acaatggacg
   126061 agggaagaat cgtttccgaa aaagaaaact tagttacaga atgtcactat ctaactcacg
   126121 aagagtttat aaaagacatg tatcagttaa tgaatgaaca gttgatgtat gttgtatgga
   126181 acaaatgcta tcgtagcgat attctgaaaa ataaccaaat tctatttaaa acttataata
   126241 gttgtgagga tcgaatattt aacttacatt attatgaata ctgtcatcaa gtgatcatga
   126301 acccaaaagt agaatatatt
//
LOCUS       DARBEG010000002        68369 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000002 DARBEG010000000
VERSION     DARBEG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.002"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            31..912
                     /locus_tag="SCK20_000127"
     CDS             31..912
                     /locus_tag="SCK20_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144226.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            925..2535
                     /locus_tag="SCK20_000128"
     CDS             925..2535
                     /locus_tag="SCK20_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG1144227.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2612..3271)
                     /locus_tag="SCK20_000129"
     CDS             complement(2612..3271)
                     /locus_tag="SCK20_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1144228.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3554..4219)
                     /locus_tag="SCK20_000130"
     CDS             complement(3554..4219)
                     /locus_tag="SCK20_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG1144229.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4366..4971)
                     /gene="dhaL"
                     /locus_tag="SCK20_000131"
     CDS             complement(4366..4971)
                     /gene="dhaL"
                     /locus_tag="SCK20_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG1144230.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4999..6009)
                     /gene="dhaK"
                     /locus_tag="SCK20_000132"
     CDS             complement(4999..6009)
                     /gene="dhaK"
                     /locus_tag="SCK20_000132"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG1144231.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5984..6430)
                     /gene="dhaM"
                     /locus_tag="SCK20_000133"
     CDS             complement(5984..6430)
                     /gene="dhaM"
                     /locus_tag="SCK20_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG1144232.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6533..6853
                     /locus_tag="SCK20_000134"
     CDS             6533..6853
                     /locus_tag="SCK20_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG1144233.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6965..7834)
                     /locus_tag="SCK20_000135"
     CDS             complement(6965..7834)
                     /locus_tag="SCK20_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1144234.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7930..9233)
                     /locus_tag="SCK20_000136"
                     /pseudo
     CDS             complement(7930..9233)
                     /locus_tag="SCK20_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9230..9739)
                     /locus_tag="SCK20_000137"
     CDS             complement(9230..9739)
                     /locus_tag="SCK20_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144235.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9914..12049)
                     /locus_tag="SCK20_000138"
     CDS             complement(9914..12049)
                     /locus_tag="SCK20_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1144236.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12132..13787)
                     /locus_tag="SCK20_000139"
     CDS             complement(12132..13787)
                     /locus_tag="SCK20_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1144237.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13789..14103)
                     /locus_tag="SCK20_000140"
     CDS             complement(13789..14103)
                     /locus_tag="SCK20_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1144238.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14273..14533
                     /locus_tag="SCK20_000141"
     CDS             14273..14533
                     /locus_tag="SCK20_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG1144239.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14547..14861
                     /locus_tag="SCK20_000142"
     CDS             14547..14861
                     /locus_tag="SCK20_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG1144240.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15591..16145)
                     /locus_tag="SCK20_000143"
     CDS             complement(15591..16145)
                     /locus_tag="SCK20_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG1144241.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16271..18199)
                     /locus_tag="SCK20_000144"
     CDS             complement(16271..18199)
                     /locus_tag="SCK20_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1144242.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(18505..19221)
                     /locus_tag="SCK20_000145"
     CDS             complement(18505..19221)
                     /locus_tag="SCK20_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1144243.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19222..21045)
                     /gene="glpO"
                     /locus_tag="SCK20_000146"
     CDS             complement(19222..21045)
                     /gene="glpO"
                     /locus_tag="SCK20_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG1144244.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21059..22555)
                     /gene="glpK"
                     /locus_tag="SCK20_000147"
     CDS             complement(21059..22555)
                     /gene="glpK"
                     /locus_tag="SCK20_000147"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG1144245.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22691..24151)
                     /locus_tag="SCK20_000148"
     CDS             complement(22691..24151)
                     /locus_tag="SCK20_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144246.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24141..25436)
                     /locus_tag="SCK20_000149"
     CDS             complement(24141..25436)
                     /locus_tag="SCK20_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1144247.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25768..26445)
                     /locus_tag="SCK20_000150"
     CDS             complement(25768..26445)
                     /locus_tag="SCK20_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG1144248.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26475..27566)
                     /locus_tag="SCK20_000151"
     CDS             complement(26475..27566)
                     /locus_tag="SCK20_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG1144249.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(27667..28101)
                     /locus_tag="SCK20_000152"
     CDS             complement(27667..28101)
                     /locus_tag="SCK20_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1144250.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28685..30142)
                     /gene="ascB"
                     /locus_tag="SCK20_000153"
     CDS             complement(28685..30142)
                     /gene="ascB"
                     /locus_tag="SCK20_000153"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1144251.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30139..32034)
                     /locus_tag="SCK20_000154"
     CDS             complement(30139..32034)
                     /locus_tag="SCK20_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1144252.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32092..33867)
                     /locus_tag="SCK20_000155"
     CDS             complement(32092..33867)
                     /locus_tag="SCK20_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1144253.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33917..35413)
                     /locus_tag="SCK20_000156"
     CDS             complement(33917..35413)
                     /locus_tag="SCK20_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1144254.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35607..37229)
                     /locus_tag="SCK20_000157"
     CDS             complement(35607..37229)
                     /locus_tag="SCK20_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1144255.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37678..38991)
                     /locus_tag="SCK20_000158"
     CDS             complement(37678..38991)
                     /locus_tag="SCK20_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1144256.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39236..39460)
                     /locus_tag="SCK20_000159"
     CDS             complement(39236..39460)
                     /locus_tag="SCK20_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144257.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39480..39902)
                     /locus_tag="SCK20_000160"
     CDS             complement(39480..39902)
                     /locus_tag="SCK20_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG1144258.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(40046..40756)
                     /locus_tag="SCK20_000161"
     CDS             complement(40046..40756)
                     /locus_tag="SCK20_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144259.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40774..41271)
                     /locus_tag="SCK20_000162"
     CDS             complement(40774..41271)
                     /locus_tag="SCK20_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1144260.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41308..41760)
                     /locus_tag="SCK20_000163"
     CDS             complement(41308..41760)
                     /locus_tag="SCK20_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1144261.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41895..42500)
                     /locus_tag="SCK20_000164"
     CDS             complement(41895..42500)
                     /locus_tag="SCK20_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1144262.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42950..43570)
                     /locus_tag="SCK20_000165"
     CDS             complement(42950..43570)
                     /locus_tag="SCK20_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1144263.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43572..44441)
                     /locus_tag="SCK20_000166"
     CDS             complement(43572..44441)
                     /locus_tag="SCK20_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG1144264.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44464..44871)
                     /locus_tag="SCK20_000167"
     CDS             complement(44464..44871)
                     /locus_tag="SCK20_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1144265.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44890..46179)
                     /locus_tag="SCK20_000168"
     CDS             complement(44890..46179)
                     /locus_tag="SCK20_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1144266.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(46206..46508)
                     /locus_tag="SCK20_000169"
     CDS             complement(46206..46508)
                     /locus_tag="SCK20_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1144267.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(46533..48626)
                     /locus_tag="SCK20_000170"
     CDS             complement(46533..48626)
                     /locus_tag="SCK20_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1144268.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(49115..49879)
                     /locus_tag="SCK20_000171"
     CDS             complement(49115..49879)
                     /locus_tag="SCK20_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG1144269.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(49990..50859)
                     /gene="mdcB"
                     /locus_tag="SCK20_000172"
     CDS             complement(49990..50859)
                     /gene="mdcB"
                     /locus_tag="SCK20_000172"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG1144270.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(50884..51960)
                     /locus_tag="SCK20_000173"
     CDS             complement(50884..51960)
                     /locus_tag="SCK20_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG1144271.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(51984..52577)
                     /locus_tag="SCK20_000174"
     CDS             complement(51984..52577)
                     /locus_tag="SCK20_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG1144272.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(52581..54257)
                     /gene="mdcD"
                     /locus_tag="SCK20_000175"
     CDS             complement(52581..54257)
                     /gene="mdcD"
                     /locus_tag="SCK20_000175"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG1144273.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(54247..54546)
                     /locus_tag="SCK20_000176"
     CDS             complement(54247..54546)
                     /locus_tag="SCK20_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG1144274.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(54648..56294)
                     /gene="mdcA"
                     /locus_tag="SCK20_000177"
     CDS             complement(54648..56294)
                     /gene="mdcA"
                     /locus_tag="SCK20_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG1144275.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(56301..57212)
                     /locus_tag="SCK20_000178"
     CDS             complement(56301..57212)
                     /locus_tag="SCK20_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1144276.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            57332..58189
                     /locus_tag="SCK20_000179"
     CDS             57332..58189
                     /locus_tag="SCK20_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1144277.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(58376..58738)
                     /locus_tag="SCK20_000180"
     CDS             complement(58376..58738)
                     /locus_tag="SCK20_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG1144278.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            59084..60523
                     /locus_tag="SCK20_000181"
     CDS             59084..60523
                     /locus_tag="SCK20_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144279.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(60530..63082)
                     /locus_tag="SCK20_000182"
     CDS             complement(60530..63082)
                     /locus_tag="SCK20_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG1144280.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(63196..64527)
                     /locus_tag="SCK20_000183"
     CDS             complement(63196..64527)
                     /locus_tag="SCK20_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1144281.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(64493..65971)
                     /locus_tag="SCK20_000184"
     CDS             complement(64493..65971)
                     /locus_tag="SCK20_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1144282.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(66276..68111)
                     /gene="tdc"
                     /locus_tag="SCK20_000185"
     CDS             complement(66276..68111)
                     /gene="tdc"
                     /locus_tag="SCK20_000185"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1144283.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        19445 a        14586 c        11702 g        22636 t
ORIGIN      
        1 tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt cacacatttg
       61 aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt acattcaggt
      121 gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat tcggacactt
      181 ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa
      241 gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc tttgtgggga
      301 aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa agggagtgct
      361 caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac
      421 tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt tgattcggat
      481 ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac gattttccaa
      541 gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc tcatattctc
      601 agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac agtcgtagaa
      661 gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat agaaacagaa
      721 gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca aaaaggtcac
      781 ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga agcgtccaaa
      841 ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt catgagacat
      901 tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc cacccttttt
      961 tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg tgtcactgct
     1021 ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg attagctgga
     1081 ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac tccagcttcg
     1141 aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct tttttgcagt
     1201 ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa
     1261 cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa ctctttagct
     1321 gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag ctgtacgatg
     1381 atttttttcc aagcagattc gatcactgca gaaggtgctt ggctttttgg gctatctgta
     1441 ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact gatgccaagt
     1501 tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct tctacgagca
     1561 ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg gtcttatttg
     1621 acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt gttcagtcta
     1681 atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac cggctatatc
     1741 cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg tggtttatta
     1801 ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt gatgggggcg
     1861 gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga cttaatgaaa
     1921 cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc
     1981 gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt atttacagaa
     2041 gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa ccaactttat
     2101 tggaccaata tgttactagc tattctattt gctatattgg caatcctgat tagttctggt
     2161 gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga tctattgatg
     2221 tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg aattagtggc
     2281 tttctattcg gatggcttcc tcgaatgggt aaacttgctt atatctactt agcttactcc
     2341 tttgcactca attactttgg cggaattttg gatttaccag aatggttttc caaaacagct
     2401 gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt gttccttacg
     2461 ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag gcgaagagac
     2521 ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa atctgggtca
     2581 aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc tctttatcaa
     2641 tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat aatttaaatt
     2701 ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc attttcattg
     2761 gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag acagcgactc
     2821 gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt gttaatttct
     2881 caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag agttccgaga
     2941 aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca aataaagact
     3001 catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct attactcgta
     3061 aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg gaggccacga
     3121 tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc acatagtgat
     3181 gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg aacaaaggaa
     3241 caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa cagctatttc
     3301 taataaagaa acataacttc ccttattcca actgataatt ttcttttatc atagcataaa
     3361 cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct tgctttattt
     3421 tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat ttactttttg
     3481 aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct
     3541 tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt ggtgctagtt
     3601 cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga tagaagaata
     3661 gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct tgtttctttg
     3721 tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc
     3781 atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc gcaagcaact
     3841 ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg tacatcaaat
     3901 agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag
     3961 attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc gtccagcttt
     4021 cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc tggagctgaa
     4081 tctctttcca tttttgccag tgttctttat attctttctt gatttgttca atcttatctg
     4141 ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac aactgataat
     4201 ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt ctacctgaat
     4261 tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt cataagaaca
     4321 gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag gttgattgat
     4381 cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt cgatatgtcc
     4441 gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga ctttggtgtg
     4501 ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg cctctgtgac
     4561 tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc ggctttgaat
     4621 acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa tagaatcgac
     4681 ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac cagaagcccc
     4741 accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga ttgtttcagg
     4801 atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt tatttccatg
     4861 gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct gattgattgc
     4921 ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca tattatcacc
     4981 cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta aagaatcttt
     5041 ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt
     5101 cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca acagagctaa
     5161 aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc cgttgactag
     5221 tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa caagttcctt
     5281 tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac cgtggatacc
     5341 aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta cttctggtac
     5401 agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct tttctcccag
     5461 ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac
     5521 aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt caacggcgat
     5581 atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta attcctttgc
     5641 catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa ctccttttcc
     5701 actatccgct tccttaatag ccgtctgaat ctgatctgga gttggagaag taaacacatc
     5761 accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg atggttcatg
     5821 tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct tggctacaac
     5881 acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc cttccagcat
     5941 ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc catcctttct
     6001 ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata ccggcagtga
     6061 tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa cgttgctgct
     6121 gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta ccgatatccg
     6181 caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg attttcatcg
     6241 ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc tcttcggatt
     6301 tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta tgagaaataa
     6361 gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta acgattggtt
     6421 tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg gaagatatgt
     6481 gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc gaatgattac
     6541 aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga tcgtttttgg
     6601 tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc gaaatatctt
     6661 tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag aagcacttgc
     6721 cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg taaaaatgga
     6781 ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg gaacagctgt
     6841 ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg gacatgtagt
     6901 ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc ccatcctctc
     6961 ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca cccgcccctg
     7021 ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc tgacttgttg
     7081 atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag gtcactgatt
     7141 tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct aagtcagctt
     7201 tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg tggattgctt
     7261 gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct gtttgagcaa
     7321 cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca agatctgttt
     7381 ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat
     7441 ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg tcttctttca
     7501 atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt tccaagtctt
     7561 gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct tctgaagatg
     7621 aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt tccgaggcac
     7681 cgttttcagc accagaagag cttatagttg ttgaaggttc tgtttgagca ggttcagaag
     7741 tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt ggccagatta
     7801 acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa ctaatttctt
     7861 attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg
     7921 gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc cataacctaa
     7981 aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa
     8041 caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc gatcatctac
     8101 aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa
     8161 actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat aatgcccgat
     8221 taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa tactcaatgt
     8281 ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca tcactttttt
     8341 gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttcttt gtcgatatag
     8401 catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa aaacagataa
     8461 ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc gatcttgtct
     8521 gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc cactgtacgc
     8581 ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga tacttgagaa
     8641 ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca tatttgcagt
     8701 gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc taatcagtgc
     8761 atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc
     8821 aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa ccacacgaag
     8881 attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg ttgaaccatc
     8941 gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat actcgattgt
     9001 atcttcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga acggttcgac
     9061 actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca cataacacca
     9121 gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa aaaatacata
     9181 atagacaaac aagtatctaa gaataaatta cctgtatggg tgattgagat catttcagtt
     9241 ccaccccatt ttctttatac aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg
     9301 agacagaata aaaacggacc gtttctctct cttcttttgt tccaaaacgt tccgtataca
     9361 cttcttcaag taattcttgt ctttttttca atttttcatc ttcatattca aaagatttat
     9421 ttactttttt gtatagattt cgattgttcc acctagtcaa tacaaatgag gtaatcagga
     9481 aactgaaaaa aatcaataca aaaaagccga ttaagaaata cagcattttc ttttctgata
     9541 aaaaagcgta caaggaaaaa tccttggctc tttctggtct cgttaatggg aaaaagaccc
     9601 atataaaagg aagaacaacg atcatccatc ctaaaagagc cactaacgtt tgccaaatct
     9661 ttaaccagta attacctttt gaaaaatact tatctttcac caaaattgat gcaacttctt
     9721 ctcttgttac ttttttcata tcctcacctc acgttttttt gttacgacgt aggagtttac
     9781 catacatttc atttcttttc acataaagtg ttcctaaatt tttcctaaga aaaagaacaa
     9841 aacatttctc atcagcaaat gaaaaatatt ttgttctttt tatcagttgt tctttttatt
     9901 ttttatctct taattatgtt gctctttcca atacgacaat gcttttcatt cctctagata
     9961 tcgccgtgta catttgtctg cgtcttcttt gctcatctcc atcgttttca aatggatata
    10021 agattacatg atcaaattcc agtccttttg ctagactcaa aggaatgatt tggcagtttt
    10081 ctaatccata tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt
    10141 gggtgataac tgccgctttt tctcctttta gagaaaccaa aatctcagct agtattttca
    10201 acaattcttc agaggatcga aaagagcggt attcgggttc ttttccttgc ggtcggatcg
    10261 atacgatgtc gatcttctct tgatccacag cataggtttt gaaaagctct gtgattgctc
    10321 cgctagaacg gtaactattt ctaagatcat accgatggat cggcagatgg tagtcgtcga
    10381 aacaacgcat aatatcagca aaagtagtgg aagaattaaa gattgcctga ttctcgtcac
    10441 ctactaaagt aaagtgagtt tttggaaata attcgatcaa tagacctaat tgtgccaggg
    10501 tataatcttg tacttcatca atcaatagat agcgaaatgc tggtacattt accttttcaa
    10561 caagtaaatg acgaatagct aatataatga ctgcttcatc aacagtacgt gtgggttgcc
    10621 aagcataagc tgagccatat cgtttttcat aaattctttc aaacaactga tgctcatcca
    10681 cccatgccat ttcttcgatt tgtctggtta tttttcgata ttttttttgt aaaagtttcc
    10741 ttgcataagc tacaatactt tgttccgaat catcagaaat caatttaccg aataattcca
    10801 actgcctatc ttctgtcaac gagagtacct gattctgcac agcaggactt tttgcttgtt
    10861 tcaaaagatg attcttccat tcgctcatca gtgccttttt cgttgcttga agcctatcga
    10921 tcaatttagg atgagcagga actttctcat atattttttc gatcgttttt ttagaaagga
    10981 ctgtttttcc atttttagta agtccacgga taaaattcgg atgatctaaa aacaaaggat
    11041 ctgattgttt tagattatcg ataaactttt tgctacgcaa acgttctgtc tgctccgata
    11101 cattttctcc cgtgatacgt ttgaaataag cctcttcacc ttctatttcc tctgctgaaa
    11161 gctgtgaaac cagctgtatg atggttagat tcagtggatt tctttcacct aatgatggta
    11221 ggacattcga aatatagtcg ataaaccgat gattaggtga tagaatcagc aagtcatcgg
    11281 atgtcatcgt atctcgatat aaatagagca gatacgcaat acgctgcatg atagcagagg
    11341 ttttaccact tcctgctacg ccatttacca agatattttg acttttcaca tcccgaatga
    11401 ttgtgttttg ttccgattgg atcgtagcag tgatagcctt catttcattg gtctcatctt
    11461 ggcctaagac ttctaataat acgtcatctt gaatagctac agacgtatca aaaaacttga
    11521 ccaaacgatc tctttccaaa agaaattgtc gtctttcttt aataaccgta tcgattttat
    11581 tttgacgaac cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg
    11641 gtgaacgcca atcataaaca agattttcat ctgcatcatc tgtgaaattt gctgtaccga
    11701 tataaaagga ttcttttggc tcattatcca gaaagtctac aacgactttt ccaaagtagg
    11761 gacttttcaa taaacgttcg atctttgcta gctgattttc tgcgtttttc tgtttgatat
    11821 tcatctgatc gatttctcta tttttcattt caatcatcga aaaagtatcc aggttatcta
    11881 agtaactgtc aaaattcaat cgaagctcgc cagccatttc tttcaacgac tggtttcctt
    11941 ctatttttgc cgtatccatc gattcccgca gttcgttttt tttatcaatt agcttttgat
    12001 acacgttctt cagatatgct tcttctaact gtttttgtgt gttgaccata cacattctcc
    12061 tcttttttta aagtggtgga accgattagt ttacctcttt taagagtcga atgcaagctt
    12121 tttaacgagt atcatttgtt aggatatatc agttcttcag gtcggacagt ttgatataaa
    12181 gaaaatgcac catcaaggtt ttttacgtgg tagccagact gcttcaagat tctttccgct
    12241 agatagctac gtaatccgct atgacagctg acgatatatt cttgttggct gtctaattca
    12301 tttaatcggt cccgaagttc attcaaaggg atatgtttgg cttccttgaa tttgcccttt
    12361 tccaattctt ctgctgttcg cacatctaat aaaatttttc cttttgctag ttctgtcggc
    12421 aattcatgcc attgtacggt ttcactaagc ccttctgcaa tgttcattgc tgcatatcct
    12481 agcatgttta ctggatcttt ggctgagcca aatggcggtg catacgtcaa ttctaattcc
    12541 ggtaaatcga agatcgtcaa atgccctttg attgctgttg ctaaaatatc gatccgttta
    12601 tccacacctt ttgccccaac accttgtgca ccataaatct cgccagtctc tggatggaag
    12661 atcaacttca gtaaaatatc tgttgcttct ggataatagc ttgcatgatc ttttcctgat
    12721 acatgaatga cttcaaaaga cagttcgttt tctttggctg ttctttcact taaaccggtg
    12781 gatgcggcag tcattccaaa cgttcgtata attgctgttc caataccgcc ttgatttctt
    12841 cgagaaattc cggaaatcgt gtccgcaact tgtctacctt gacgattggc tggtgaagca
    12901 agagaaataa gtgcatcttt tccagtgatt tgctgtttta caacgatagc atcccccacc
    12961 gcataaatat caggaatatt cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg
    13021 cctaattcga ttccagcggt cactgctagc tggttatcag gacgaacacc aatggataaa
    13081 ataactaaat cagcagggat ggtacgccca tcatccagtc gcaatcgttt cccgtggtct
    13141 tcaaatccag cgacagcatg agaagtaatc acttctactc cttgatgaga caattctttt
    13201 tctataaaag ctgccatttc ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc
    13261 accatgactt ctaaacctct tctttttagg ttttctgcca tttccaatcc gatgaatcct
    13321 gcaccaacga tcacggcacg ctttggctgt ttttctaatg catgcatcac tttatcgata
    13381 tctggtacat ttctaataga aaaaacattg tcggcttctg ttatccctgg aatcgatgga
    13441 acaactggtt ttgcaccagg agataaaacc aatgcatcgt actgttcaat gtgtttttga
    13501 ccctcatgta cgacttctac cgttttgttt tcgggaaaga tggctgtcac ttcatgattt
    13561 ggacgcacat ctaaacaaaa acgagcggat aatgattctg gcgtttggac aagaagattt
    13621 tcccgttcac tgatttctcc cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca
    13681 tacggtcctt tttcaaaaac aacgatttct gcatcttcca ttaatcggcg aagccgtgta
    13741 gcagcagaca ttccacctgc tacacctccg acaataacga ttttcatctt atttccctcc
    13801 tactgttcga tcaaaccatt gattcatgcc accacgtaca ttcactacat cataaccaga
    13861 tttctttaat tctttagctg cttgtttact cctcatccct gattgacaaa tcacatagac
    13921 ggtcttttct ttgtctccat catatctgtt tatactttgt aatggaacat ttttagctcc
    13981 ttttatatgc ccgccccggt attctgcagg tgttctaaca tcaagtaacg ctggattttc
    14041 caaaagcttt tcctttaatt ctgggactgt tatcgatttt attgattgaa atagcaaatt
    14101 cattttctgc acctcattta catttttata aatacctcat ggggtatatt aaacaggagc
    14161 tttattaata tgtcaaccag aatgactctt catttttttc actacttttt atatttgcat
    14221 aaaataccga taaggggtat tatctaatta taaaaataga ggagaatgtg ttatggagat
    14281 tgataataaa aatatcctga accgtttgaa acgtacagag ggacaaattc gtggtatcca
    14341 aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc actcaattaa gcgctgttcg
    14401 ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa attgctttga
    14461 gaaacctgat agtaatccag aagaacaagc ggctaaacta gaacaagcga tcaagatgat
    14521 tattaaaaag taaaggaatg atttcaatga aagtcgtttt ccatattgat gaattagaaa
    14581 aatggtcaga aacagggaaa aacgtaaaaa acctgataaa agcttctcca gaaacgacga
    14641 ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct agactctgcc aatgcagaat
    14701 tcttagattt gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt gctaatcata
    14761 tttcagaaag ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag
    14821 ttgaactgca atcccaaggt tatgcttata tcaagcctta gtcagtagag gttgcgacag
    14881 aagtgatcag cttcaagaaa tttcggctta tttttcgaag aagctacttc tggaaaaccg
    14941 tgaatcaaag gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc caaacaaaaa
    15001 tagcttctca tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
    15061 cgtttaagga agaggttgtg aaatcagcgg tttgacctaa gtattagaga aaaaaacggg
    15121 aaaatagctc ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac
    15181 accgtttaag gaagagattg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg
    15241 tgaaaatgga tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
    15301 aataccgttt aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa
    15361 acggaaaaat agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat
    15421 tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa
    15481 tttcaaaaac aacttctcct gtttttggtt tggttggctt attatcgaga agtagctact
    15541 gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt
    15601 tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg
    15661 atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg
    15721 atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc tccacttaat
    15781 ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag
    15841 aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa
    15901 agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta
    15961 tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct
    16021 cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata
    16081 cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat
    16141 ttcattttat cgccctacct cctcagcact cttttctttt tattttagct tacacgggga
    16201 ataaaaccaa tgatttcact gtttttattt ttctttacag ccactgatat tatgagctaa
    16261 tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca
    16321 tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt
    16381 tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt
    16441 ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat
    16501 atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat
    16561 tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc tccttcctat
    16621 ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca
    16681 agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca
    16741 aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga agtgcttccc
    16801 gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat ttcttgattt
    16861 ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat tccatgattg
    16921 cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca tctaaaagta
    16981 ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata tgaccagaaa
    17041 atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa atctcatcaa
    17101 agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc gccatcaaag
    17161 tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct ccggtgataa
    17221 tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc tcaggtaaaa
    17281 atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta ggtgtgcttc
    17341 cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc gtgatcgttt
    17401 cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat gccgccagga
    17461 cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca ttcaatttag
    17521 aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg ataatcgttc
    17581 catctatttt atttttatag ctggctttga taggaatcgt atagtattcg cctttttgaa
    17641 caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat ttcagtaatt
    17701 ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc aaattacgtg
    17761 acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct tctacattta
    17821 tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat tgattcttat
    17881 caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg atcaaatcgg
    17941 ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc ctcggcaacc
    18001 ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt gtttcttctt
    18061 gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt cgttttttgc
    18121 tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat tgtaattgac
    18181 taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc ccaaaataaa
    18241 taagggccta gagataaata aaaggataga acacagctta aaaaagtcat gtcctatcct
    18301 tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca caccaaataa
    18361 actttgttta ttggctattc gacttactga ttagatacca gctacactca caaaacatac
    18421 cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca ttttggttgt
    18481 caaactttag tatgttttat agatttacac cattaatgaa tagacaccta ccgcgataat
    18541 tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg aagttccttt
    18601 attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag cagggttgat
    18661 tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc cacctacagc
    18721 gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc caagaactaa
    18781 tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta tagcaggacc
    18841 tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat gtggcaaata
    18901 agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa tgatgaaagg
    18961 aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga ctgcaggatt
    19021 caaatgagct gggcccatga atccagaaat atagacagcc attgttacag cgagtcccca
    19081 accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta aattcacagc
    19141 cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact ctcccatgat
    19201 ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat ctgattctgc
    19261 aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc attcaaaata
    19321 tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc gttcaaacaa
    19381 cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt cttcttctaa
    19441 accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg ccatttcttt
    19501 tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat aagtagcatc
    19561 ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg tttcttctaa
    19621 tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga tttctttcgt
    19681 ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc cttctgccat
    19741 cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg gttctctttc
    19801 taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt tttccaaatg
    19861 gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt cacttactgt
    19921 atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac cattgtaatc
    19981 agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt cgatatcatt
    20041 caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat aatctacatc
    20101 ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg tcgtaccaaa
    20161 gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct ttcctgtatc
    20221 gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat ggatcccttt
    20281 agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta ctttatctac
    20341 ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt catctgtcag
    20401 caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc caaccgcttt
    20461 cattttactt actagataag ccccatcttc ggcagccttt ttgatattat caatcactaa
    20521 acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc cttctttttt
    20581 caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct catatttggt
    20641 tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg agaacatatt
    20701 gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg gttttgggat
    20761 gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta ctacttccac
    20821 atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc gagaggaagt
    20881 cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg ctgctgactg
    20941 gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca attcttcttg
    21001 ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga tttcctcctt
    21061 attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt ttttctcggc
    21121 gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg cggatctcgt
    21181 ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct agcgccgttg
    21241 tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg aattgcatca
    21301 gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg attccagtgt
    21361 catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt aaagtagctt
    21421 taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct cgtgctgcct
    21481 gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc tcatcttcgc
    21541 ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt tgaagtccat
    21601 ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa gcatagtaaa
    21661 cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat aattgcggtt
    21721 tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg accatgcctg
    21781 gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc cctgaaattg
    21841 gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct gagttagaag
    21901 tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc tgatcccaat
    21961 ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca gtaacatgtg
    22021 cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac atcaattcgc
    22081 ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt atcttcgttg
    22141 ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct tgatagcctt
    22201 cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca actgcatgat
    22261 agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg ttcgttatgc
    22321 caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg gcaataactg
    22381 actgtacaga attccagatt tcatttgcat catgttccac ccaactttct tgcgggaaat
    22441 attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca aagataattg
    22501 ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct gtcattgtga
    22561 ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac tgttttaaaa
    22621 aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt attatgaagc
    22681 tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc aatcgaataa
    22741 tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg tttctctggc
    22801 aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc tcaataccgc
    22861 cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa atcggcagac
    22921 tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc atcaaaagaa
    22981 cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt ttcctcggca
    23041 attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa tgcccgagca
    23101 ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt ccaacaaaga
    23161 aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct tctttgattt
    23221 tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta tctaaaacag
    23281 cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct tcgtctacta
    23341 tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc agttctgctg
    23401 ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc cggtccgact
    23461 gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc aatagtcttt
    23521 ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta aacgttgttt
    23581 ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt tgcaaaaaaa
    23641 gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat cgtatctgta
    23701 gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct aagagctggt
    23761 tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct gtcgctaatc
    23821 gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac ttcaagcttt
    23881 cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac acaagcagcg
    23941 cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca atcaattcaa
    24001 tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct ctcaaaccat
    24061 tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc tgtaactgct
    24121 cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt tgaccacacc
    24181 atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa ctgttcaaac
    24241 gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat ctcttttttg
    24301 ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt gagtttaact
    24361 ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat tttttgggga
    24421 taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc aaaccactga
    24481 ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat tgtcttgctg
    24541 acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt ttcatttgtt
    24601 ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg agacgtttgc
    24661 aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc ttcccacatc
    24721 tgattgtcgg ggcgagaatg attagccagc aaaacaagat catagttctc ctttcttttt
    24781 tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc ttctaaaaaa
    24841 agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc aggatctaga
    24901 taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt tcttgatgcc
    24961 aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat tttctttgca
    25021 ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg gtgaataagc
    25081 gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag aatcaaacga
    25141 tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa tcctattact
    25201 gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt cgtgatccaa
    25261 gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc gctgggaatc
    25321 catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat caattggcga
    25381 agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat gttcatctgc
    25441 attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac acagtattca
    25501 taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct ctccatcata
    25561 ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag agtatcattt
    25621 ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc ttttacgtac
    25681 ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg tgtaatgtaa
    25741 tagttagaat agacacatca tttattttta attctttaat gcttcaacaa ctgcttcgcc
    25801 taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa tttgaaccac
    25861 ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag catcttctgc
    25921 taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac tgaacccagt
    25981 cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca acaatcctac
    26041 aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg aagcgacttt
    26101 cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag cataataaac
    26161 cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca aggcccgatt
    26221 tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca aaacactttg
    26281 cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga tcccattttt
    26341 tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag gacgctcgat
    26401 cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc catcctcttt
    26461 ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg gcttggcaaa
    26521 taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc acggttttcc
    26581 aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc tgcaagttct
    26641 gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac accaactaaa
    26701 gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc gttgtattta
    26761 tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc tagttttgac
    26821 tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg tgtttcatat
    26881 tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg ttcaaccgct
    26941 gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa gctaccgccc
    27001 cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa atacgtattt
    27061 gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt ggctttgcca
    27121 aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt ttccgcatca
    27181 ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa agttgctcga
    27241 cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc tccttggatt
    27301 gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct gagttcacct
    27361 ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc accaagacct
    27421 ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt gaaaaagctg
    27481 agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc tgaaggtaaa
    27541 gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc tgattaacct
    27601 atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta atttgcttaa
    27661 aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg ctaattcttt
    27721 acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc agctaccatc
    27781 gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca agcgaccttc
    27841 gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag caatcgttga
    27901 tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt tttcttttaa
    27961 tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg cttcatcagc
    28021 taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt catctaacgc
    28081 tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat agtatgattt
    28141 ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat ttgaaaaaaa
    28201 gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc tggaacaccg
    28261 tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat tttgtgaaaa
    28321 tggctttcca catttttgca taaatttggc ttaataccga aaaactggct tttgaacacc
    28381 gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac agccaaacaa
    28441 aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc tgattgtaca
    28501 ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga gcaatttcaa
    28561 aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt ggcaggagaa
    28621 tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc cttatttcct
    28681 tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca aatgattttt
    28741 tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag atatatccat
    28801 aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc cacgacgtat
    28861 aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata tgctgtttca
    28921 aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg tctatagctc
    28981 cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat tgattcaaag
    29041 caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga agatacggat
    29101 ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct ggatgagcac
    29161 tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag gctgctaaga
    29221 tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac attcgttgcg
    29281 tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa gttctttgct
    29341 gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga agctgatagc
    29401 tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt gtagctaaag
    29461 cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt
    29521 tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg atctcattga
    29581 aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca gcgaaacgaa
    29641 cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc aaatataacg
    29701 gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga cattcgtcaa
    29761 acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca ccatgaggaa
    29821 aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca gcaaataaac
    29881 ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga taataatact
    29941 tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc cattgatctg
    30001 tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca gataatcctt
    30061 tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg ccccataaaa
    30121 atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc ttgtttgatt
    30181 atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa caataaccat
    30241 tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca tcaataaatc
    30301 gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc catttaaatt
    30361 cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga ctaccccaat
    30421 tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acaggactgt agatttttcc
    30481 gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg ttggatcgtt
    30541 tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta tatcctttga
    30601 ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac aggaactgat
    30661 catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat aaatcgggaa
    30721 agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac cagctactcc
    30781 accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt taagagtcac
    30841 accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag ataaagataa
    30901 actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc ctgcttgcgc
    30961 agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag aaagattgtt
    31021 ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt gccaaaatcc
    31081 gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat atccagctgc
    31141 aatcagctga cttgcataat ctgcaattgg acctacaact aataaagtaa ttgggaccat
    31201 cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca agcttttgcg
    31261 aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa taaccgtact
    31321 tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc tatcactata
    31381 tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga tgaaacgatc
    31441 tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat agaaaaatgc
    31501 atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata aattcaaact
    31561 cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc ctaaagtcgg
    31621 ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt tctcagttcc
    31681 tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata cgtcatacac
    31741 ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga ctacagtgat
    31801 cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt ttagggtaaa
    31861 gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg
    31921 gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt taattaatgc
    31981 cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct ccattcaatt
    32041 agtacccctt ataatagcga acgaaactgt acaatagtgt gtatggttta cttagccgag
    32101 caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt caaaggagac
    32161 aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa gctgatactc
    32221 tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct cggcatttaa
    32281 cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat ctatcttttt
    32341 atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg ttttcttttt
    32401 ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag attcaaactc
    32461 atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct cttgctcagt
    32521 agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa gatacactgg
    32581 ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt tttttagtgt
    32641 attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt ttcgaaaagg
    32701 attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa
    32761 ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat aacgggagat
    32821 gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg ttttcatttg
    32881 tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag gaagatctaa
    32941 aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt tgtttttttc
    33001 atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt gtttttcttg
    33061 attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct ctaccatcgg
    33121 aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa gaatggtcaa
    33181 aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat tatatgatat
    33241 aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa ttgtactgat
    33301 ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt ccaacaagag ataagaaaat
    33361 tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa cttccagttc
    33421 taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg atacggctgt
    33481 ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt tttgaaacaa
    33541 taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt tatgtttcgt
    33601 cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa aataacctga
    33661 acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat ttttgatttc
    33721 agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc ttgttatcca
    33781 atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta agtccatttg
    33841 cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac aatagtcact
    33901 tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta ccattagact
    33961 ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag cttccgctcc
    34021 catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc cctgttcctg
    34081 ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa ccatctttta
    34141 ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt ggatcgtgga
    34201 ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact atgaataatg
    34261 gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga ggatcaattg
    34321 gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa ccatttgctc
    34381 cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga taataactaa
    34441 atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct aacattactg
    34501 gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc cctcggcaca
    34561 tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat acattctctg
    34621 gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata cccatcttag
    34681 catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga aacatatgat
    34741 gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt ttatctctag
    34801 cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta ttgatctcat
    34861 taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc tgcgcataaa
    34921 attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct accaatgcta
    34981 agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga tttaatgtat
    35041 tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg tctcctcttg
    35101 gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac tccttcagta
    35161 aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa agataatgga
    35221 gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga atactaggat
    35281 gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg tcttccaacc
    35341 atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt tctggaaata
    35401 tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga agtatagctc
    35461 gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa aattgaatgc
    35521 tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc gaaagaaaac
    35581 actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc ttcataaggt
    35641 cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac aataaccctt
    35701 aaagcttcac gtggaacagt gaacgtctgc ttttcactgg acatattaca aataacgagc
    35761 cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt cttttccact
    35821 aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat caatttcttg
    35881 tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat ttctttataa
    35941 tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc acgggcgttc
    36001 cattgcatag gcgtcctagc attgtctctt cctttcgcat tgattgattc caagatttcc
    36061 tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc aatatctttt
    36121 gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc ttgatagata
    36181 taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact ttcttttcga
    36241 tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt attccaaaat
    36301 aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac ttttttcagt
    36361 tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg atctaatcct
    36421 atgtgttcga attggaagac catggataat tcatttcttt taggattgga ataaagttta
    36481 gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa
    36541 gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact tgtgatttct
    36601 ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc aataccttta
    36661 tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt tttccaattc
    36721 aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt ttcatccaac
    36781 tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc attgaccgga
    36841 tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc ctcaatgaac
    36901 cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg gatatttctt
    36961 ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc tgccatgatt
    37021 gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta tactggagaa
    37081 agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat aatcccccgc
    37141 aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat ttgataaaca
    37201 actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga tgtttctatt
    37261 ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt tcaaatagct
    37321 attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa attccctcaa
    37381 ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa cttctttata
    37441 aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa tatgaggaac
    37501 tattttttcg tttttttctc taatactcag gccagaacac tgatttcaca acctctttat
    37561 aaacggtgtt cactcagctg tccctcggta ttagctcaaa aaacggcaaa atataagaaa
    37621 ctattttttc gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta
    37681 tttcacattc aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg
    37741 agatacttct tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa
    37801 tgcaatccca tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg
    37861 gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc
    37921 taccataatc aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa
    37981 gaacaagcct aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc
    38041 aacggcgata ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga
    38101 acccgcaagc atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt
    38161 gtctttcatg aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa
    38221 agtcacaagc aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac
    38281 atgttttaat gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga
    38341 aggcattgga atcaaacctg tgaccagacc taaaatcgtt gtagctgcta tcccgatgaa
    38401 gatgccacct ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt
    38461 caaccaagta gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac
    38521 gatgatccca ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat
    38581 cgcatatttc aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt
    38641 gatcaaaata aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat
    38701 ccccattcct aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt
    38761 tccaattgga taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc
    38821 agtcgcagta aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac
    38881 aaataagata taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac
    38941 cgtggtgttc aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc
    39001 ccctaaatcc gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac
    39061 gtgttttatc atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa
    39121 atccgaacgt tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg
    39181 attacaagaa tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat
    39241 agtgtgattt gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga
    39301 caggattcag attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg
    39361 tcttcttttt tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc
    39421 gatacttcaa ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt
    39481 caatcagacg actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg
    39541 aaagtcaaga tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata
    39601 gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa
    39661 aacttctgca caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt
    39721 acaatactct taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt
    39781 tctacaaaga atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt
    39841 tgtatcatca tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc
    39901 attatatcac gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat
    39961 agtttctagg attgctttcc tccacaaact ttttttacaa aaaaaaaaaa cggcaatgaa
    40021 tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct ttggttagtg
    40081 tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac gcttcgattg
    40141 cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc accgctgctt
    40201 gtgtttcttc cgtcagcatt gttctttggg cttcacgcca gttgagacag cgacagatcg
    40261 ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg gaatctgcac
    40321 ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt cctgcgatct
    40381 tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta ttatagatat
    40441 ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc agtaaagctt
    40501 caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga cgccactgag
    40561 caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct tcttttgctg
    40621 cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg ttatcgatac
    40681 catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa ttggtatcca
    40741 ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga tgaatagata
    40801 gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga taaatccttt
    40861 tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc aaatggctgt
    40921 tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac cgataccttg
    40981 atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat cagccatttc
    41041 ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt gatcgatact
    41101 cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt ctagtgcata
    41161 agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa atgtatctgt
    41221 aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca tttttcgaat
    41281 ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt ttttgacaaa
    41341 attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca attgttttgc
    41401 ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt ctcgaatgat
    41461 gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt ttttattttc
    41521 tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg ctcgggcagt
    41581 cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc caggattttc
    41641 gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt tgaattcgat
    41701 attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga tttcagccat
    41761 ttcatcccac ctttattttg tttgtaagac tagtttacct ctttttgttg caaatgcaac
    41821 tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat tcttttcttc
    41881 ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat aagtaactgt
    41941 ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa aaatcccatg
    42001 ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc acaagcaaaa
    42061 acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt atcaaaaact
    42121 gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc tttacgcaca
    42181 ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca ttctttcgtc
    42241 cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg aacatctact
    42301 tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc gaaagtttct
    42361 tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa acgctcttta
    42421 tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag tcttttgtat
    42481 tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg actatgtaat
    42541 caagtataga agactggttt aattacggca aataaatata atgaaatctg caataaataa
    42601 aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct gtgaataaat
    42661 ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat atgacgagct
    42721 attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa cctccccact
    42781 aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca aacatgagaa
    42841 gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc acgacctctc
    42901 ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt tacttattgt
    42961 cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga gtatagtctg
    43021 ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg tcggcgatga
    43081 tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct tccaatacgt
    43141 caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca cgatcacgtt
    43201 tgtaccgacg ttgacataac acttcaaagt ctgctgttag gcgaatcgtg atcacctgat
    43261 atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta aaaggatact
    43321 cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg taatagaaag
    43381 gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac ccgatggatt
    43441 ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga aatcgttccc
    43501 gtagtttatt cataagatat gtttttcctg taccaggact accagccaga agaatgaaat
    43561 actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat aaacagtcca
    43621 atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt atggcgaaga
    43681 gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca gttgactaaa
    43741 agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg ataatcttct
    43801 tctttcacac ctgttcctcc atggataact agggggacat caactactgc atcgatttct
    43861 ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt cgtaccgata
    43921 gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc ttccggtctt
    43981 gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc gattgttcca
    44041 agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc ttttgtcttc
    44101 aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt atatccgctg
    44161 tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa gactgcatca
    44221 accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat gccgatatat
    44281 ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt tgctgcgcct
    44341 agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt aaaattcaac
    44401 tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg ccaccttatc
    44461 tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct catccatttt
    44521 gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg cacgcttctg
    44581 gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat tttccaaact
    44641 cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt taagtccaac
    44701 ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct cttgtttcaa
    44761 ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt tttcttcttt
    44821 tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca tcattttccc
    44881 tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata agagtgctgc
    44941 ggctactgta tctacatcgg ttgctgtagc attgatgaat cccatcgaat taaagatcgt
    45001 tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc caatgatcgc
    45061 tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg caaagaaatt
    45121 ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa acatggcaat
    45181 aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc tataaggaaa
    45241 gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag ctatccctcg
    45301 aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata catacacacc
    45361 cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat ctcctgataa
    45421 ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg ggaccatcac
    45481 gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca acttgatatc
    45541 ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac cggcttcaaa
    45601 taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag tgattttacg
    45661 aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga taaaaccacc
    45721 gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg tcgtcatcgt
    45781 tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg tgaaacgagc
    45841 caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc tacctaaacc
    45901 caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc cttgcatatg
    45961 aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga tgctcgaacc
    46021 ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga cttgtcctgc
    46081 tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga tcgatgcctg
    46141 agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct cttttctttt
    46201 tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc aatttcattt
    46261 tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc gaacttttcg
    46321 aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga aactgcggaa
    46381 gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac atcctcagca
    46441 gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa aatgttcaat
    46501 ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga aaaaagcagt
    46561 tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc ttcgttgttg
    46621 atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac tgctgccaaa
    46681 caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg ttgctcaatc
    46741 gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg ggctagtgcc
    46801 atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat catcgcttcg
    46861 acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag ttcaatcgat
    46921 tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa aatatctttt
    46981 aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa taatttttct
    47041 aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac atctattatt
    47101 tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag ttcttcattt
    47161 cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg atcgacaact
    47221 aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt gacatccaat
    47281 ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat ctgaggaaac
    47341 ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt acagacaacg
    47401 actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt cgaaaagtgg
    47461 atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc ttcgatcgta
    47521 ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat gttttctttc
    47581 aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc catgtgctta
    47641 tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt ctcgttctct
    47701 acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat tgaattggag
    47761 atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc tacttgcggg
    47821 acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa gctactgtct
    47881 tttatttctg tatttttcca attaatggca gagataaatt catgtttcct gattcgctgg
    47941 atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc ttgtttcaac
    48001 aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa ataatggaga
    48061 aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc ggtcattccc
    48121 agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga acctttataa
    48181 accagtcctt gctttccata actgacgatc tctatcccat aactgaccag tgttgctcgt
    48241 aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc ttcttttttg
    48301 tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa aatacgttcg
    48361 cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg agcttgcttt
    48421 tccttttcca gcttcaacaa aaaaactttg ttgcgaattg tacggatctc cggaagcttt
    48481 ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg ctgactgaca
    48541 ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat gattctgtga
    48601 agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca agtatatggt
    48661 tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga aagtaagcaa
    48721 agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga catacgccta
    48781 ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc tgtcctgcat
    48841 caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg gttgttctta
    48901 ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca gagatgttca
    48961 gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca aaatcgtgcc
    49021 ttattgccga taggctatct ctggcacact gtttattcag aaaaaggagc tgcgacaagg
    49081 cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc ggctgaataa
    49141 aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc tgagaaaaat
    49201 actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg actgaataat
    49261 cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt cgataatttc
    49321 ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct ttgttttttt
    49381 gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg gctttttcag
    49441 gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt tccagaaact
    49501 gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct ttcagccttt
    49561 tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact gacaaaacag
    49621 ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga ccataatacc
    49681 cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat gaacgaccac
    49741 caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga ccacccgcga
    49801 cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa atgattgcta
    49861 aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa ctatttttat
    49921 gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt tttagcaatt
    49981 ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag tccataattc
    50041 ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc caggagagat
    50101 ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta aagcactttg
    50161 actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac tgcgatgcaa
    50221 tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt gtttctgtac
    50281 atatgaatca ttatctagct ccttagctgc ttggattgca agcctgatat gcggataccc
    50341 taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc tatgaccatg
    50401 agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc tgacatcatc
    50461 aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa tgacactgat
    50521 caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg tagcaatcag
    50581 cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact cctgatcaat
    50641 agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg tcttagcaga
    50701 cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg catccactaa
    50761 tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca acgcagcgag
    50821 ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc cttcttccta
    50881 ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta accagcttaa
    50941 agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc ctgaaggtaa
    51001 ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata cattggctaa
    51061 atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg gcgaatcgtt
    51121 tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac tgtataaact
    51181 ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat gtgcgagcaa
    51241 tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat aacgtactct
    51301 gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat aaatcatgaa
    51361 actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag tcaatacttg
    51421 tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca atactggcca
    51481 tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg gcatgaacaa
    51541 ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac ctgtgacata
    51601 tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg ctggaaatag
    51661 ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa acttttcttt
    51721 caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa caattgcaaa
    51781 aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa aagctgtatt
    51841 tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca taacagccgt
    51901 tacagctgat actttctgga agtcacttac gttgattttg atttttttgt tttcaaacat
    51961 ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg tccttcgact
    52021 gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga aaaaccgcct
    52081 tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga ttgaattacc
    52141 tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc actggtttct
    52201 tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat tccccattta
    52261 catcctgata atatgtttct agcagcatga aattgctgat aaacaggata atccatgatt
    52321 ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac ttgtttgacg
    52381 gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg taaaccaatc
    52441 ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat cttttcagtt
    52501 agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa aaggacaata
    52561 tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc acggacttta
    52621 tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt tgtcagcatc
    52681 gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat gactttgctg
    52741 acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata gatcgctcct
    52801 agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc agctgcttcg
    52861 atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat cgcttggacg
    52921 gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg tgctaaaaat
    52981 gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc ttgacggagt
    53041 tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa ttcttcgtta
    53101 tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt cacttttttg
    53161 tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc ttcaactaaa
    53221 ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac gattgatgtc
    53281 acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact aatagaagaa
    53341 gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact tgctgcagct
    53401 ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg gttagcaatt
    53461 gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc aagtaatgat
    53521 aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat tgctgcgatg
    53581 acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat ttttgattct
    53641 acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga atcaaattct
    53701 cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct cgcttttttt
    53761 gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa tgatcctaca
    53821 cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat tacatttccg
    53881 atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc acctgtatct
    53941 agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac atgttcaagt
    54001 gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa ttttccttcc
    54061 atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac gatcccgtca
    54121 tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg atcgaatgga
    54181 tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc gtttaattct
    54241 acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta ggtacacaac
    54301 tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat aacgatcaaa
    54361 aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg tacaaatttt
    54421 tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc ctgaaccgac
    54481 cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat aagctaattg
    54541 ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt attcgtcttt
    54601 cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta tgaccaagtt
    54661 ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc atcgatggtt
    54721 tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc ttctggtaat
    54781 ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt cattccaaga
    54841 ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt ttttgctttg
    54901 tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct ataaatcatc
    54961 actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa ttcttcgaca
    55021 aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa tttttggcct
    55081 ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga atgacgacga
    55141 ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg tccttttgtt
    55201 acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa catatcgatg
    55261 gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc gtctttaccg
    55321 acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc gccaccaaaa
    55381 ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg atgtgggttc
    55441 aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata agttggcagt
    55501 aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg tacgccgtgt
    55561 tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc tgtgatgttt
    55621 tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt cacgacaacg
    55681 tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc gttgacttga
    55741 acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt ttcttccgta
    55801 ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac taaaacaaca
    55861 tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg gatatcacct
    55921 aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc accagcataa
    55981 gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc tggtcgtgag
    56041 atactagaca tgatcaaatg cagatcattc actttttctg catctacttt ttcaagactt
    56101 tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt gtcgccggga
    56161 gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt gccattgagc
    56221 aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc ccagttgcgg
    56281 tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc gtcatcaata
    56341 ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt tgtatgaatc
    56401 agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc taattctttt
    56461 gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa caaatcttta
    56521 cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata caaacatcta
    56581 gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt atcattcatc
    56641 aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc ttggtgccgt
    56701 ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt atgttgcatc
    56761 ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt tgctacaatt
    56821 tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc gcttggataa
    56881 gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc aaatctcaaa
    56941 gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc aatatcaggc
    57001 tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag taatgctagc
    57061 tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata ccccgtcaca
    57121 ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa cttctgactg
    57181 ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc acttactttt
    57241 tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa gaaaaaacca
    57301 taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc agtattttaa
    57361 aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta tttctcaacc
    57421 tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt tgttttacca
    57481 tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat atgcagtcaa
    57541 gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg agaagatcgt
    57601 tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact tggtccgaat
    57661 ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt taccagatga
    57721 tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga ccaatattcc
    57781 tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg ctaaaaaaga
    57841 acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa agagcaacgc
    57901 cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga cgatcagttc
    57961 gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag ctgttagctt
    58021 tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc gggaaatcaa
    58081 agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag atcatccttt
    58141 gatcaaaatg attgcaaaaa cgatgcggga attttattta ggaaaataaa aaaaaaagag
    58201 ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa cggtgttcca gaagtaattt
    58261 cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc aattttcgta aattccttct
    58321 tatttcttga agctaaacat ttctgtcaca acctcttctc tattgtccta gacattcatt
    58381 gcgttgattt aaaacttcca tcaatcaaat ctgctagttc atctagcttt tctattttac
    58441 gtagatcaat agagatccaa tgttctttat tcatgtgata ggctggaaaa tacccctctt
    58501 ttcctcgcaa aattccgcca agttcaggat cgaccttcaa atcaagaata ttgatgatcc
    58561 tatggtcact tgcatcatca tagatttttt cttctggaac agacatcagg accccaaacc
    58621 attttttgct tttttgatgt ctgaatacgc aataatcagg aaatttttta aaaatatatt
    58681 ctggagacaa attgtatgtt tcattgatgt gatcgattag ttcttttttc gttagcatct
    58741 gcttccatct ctttcttatt gattcgcttt attcaaagat accacgtaag aatagaaaaa
    58801 tgaatacaag tagttgctaa tcaaatactt cgatattttt cttttttatc aactcttaaa
    58861 tgttcaatcc gcctgtgttg gtcttgctac catctactat taaaatgttt attattgttt
    58921 gcccacctat tttttgattt tagtggtatc atttttcaag ttcatgatga aattcttatc
    58981 atcagtaaat gctccagaaa aataaacaat gattgaaaaa tcagttgaaa cttgaccacc
    59041 gtttattttc tccgtattct tagaatagaa gggagtctga ttgatgtatt cactaatgaa
    59101 aaaaatcatt accgaaaaag atattcgacg tcatgtttct ttagtagaac aattgctgaa
    59161 tcatacaaag ataaccgtaa atgaattagc tgaaatcata gggacaaccg aacgaacgat
    59221 tttttctgac cttcaatcta ttcgctctca gctgcccgaa ggctgggaca tcatcagcga
    59281 tcaagcagga atcagtttgc aaaaccagca aaatttatta acaaatgacc tttgggagat
    59341 ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat ctgcttttta caaaaaaagt
    59401 tgctgtccct gattttctag ccgaccatgg gctttcttat gggacattga aaagacatgt
    59461 gactaaaatc aatcagcgtt tagcaagtta cgacttgcag atcgatttga cgaaatatac
    59521 agcttgtatt cttggaaaag agcgtgcgat tcgtactttt tatcatcgat tgctgattcc
    59581 tttcactcat aacaactatt ttttcgagga ttattcgatc catgaatccc attattttca
    59641 atttttaaga aacctcagtc aaactgaact agcggttgaa acagaagaga ttttcggaac
    59701 atgttggttc tttatcaata cgatccgtat aaaagcgaat tgccgattag attcttctat
    59761 ccataccaac agcacactct cctccctgta tgactctgca ttgaaaaaat tgtatttaac
    59821 ggaaggtatt tatcttaaag acactgagct ttcttttgct tctttctgct ttctggaaag
    59881 ttggaattac aacaataact atggacaaga aaccgctcgc tgcctgcatc attctccatt
    59941 tttagaggtc ctcgaaacgt ttgtagagga actagcttca gagctgtctc ttgatcaact
    60001 aaaaaaaaca tctctgactg ataacttggc tctcctcata ttaaaatatc atgaatcgcc
    60061 gatattgatt gaagcattgg accgtcaata ccatcgtttt ttagaaacat atgatcagca
    60121 atatgcacac ttgtatcaac agaaagagaa tttactgaac gatctaaaaa aagaaataga
    60181 aatcaatgaa ccaaattact ttctccaact cttgtctcta ctgatccaac aaacgatttt
    60241 ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc caaggagaac cttcatggaa
    60301 ggcgttcttg cagcaggaat taaaagatta cttaggaaaa cgtgtctgct tcttacctat
    60361 cgaactaacc gatctttcca ctatttcttt tgaaaaaaca gatatcttga tttccaattt
    60421 ccctctagat tctctagata tccctattgt atacatttct tctattccaa cgaaaaatga
    60481 gctcaatcgt ttgactgaac tcactttcaa aactttttta tagtaacggc tagcgactgt
    60541 attctctagc tgttttttct tttttgcgaa acagtttgtt catgatccct ttttctattt
    60601 ctacaactaa gaatagaaga atcacatttg ctccaatcaa taactgttgt gtcatcgtta
    60661 aacttactgt accgatcagc tgctggccga ctggtagaaa aataaccatc ccttgcaata
    60721 aagcaaggat acctaatgaa agaaaaagcg ctttgttttg caaaagacct tggttcaatg
    60781 acggcttgga caactctctg caattgatca tgtaaacagc ttgagctaag acgatatttt
    60841 gcagaagaat ggtttgctgt aagctttgcc cttcaaactg catagccaat aaataggcag
    60901 gaaccatgat cagtagggag acatagatga tccgaaacaa atgataacca gataggattc
    60961 cttgctttgg atctcttggt gggcgcttca tgatttctgg atctggcgac tcgaacccta
    61021 gtgcataaga taaagtgatt gtcgttacca tattgaccca taaaatctgt actggggaca
    61081 aaggtagcgg gcgattcagc ataagtgccc aaatcaagat caatccttga gctaatgcag
    61141 ttggcaagaa gaaattgatg gttttttgca aattatccaa aatccgccgg ccctctttta
    61201 cggcttttgc aatcgtatgg aaattatcat ctcctaaaac catatcagct gcctgtttgc
    61261 ttacttcact acctttgatt cccatagcaa ttcctacatc ggatcttttc aaagcaggtg
    61321 catcatttac gccgtctccg gtcattccga cgatttctcc atttttctgc aaagctttaa
    61381 caattcgcag tttatgttct ggtgtcgttc gagcaaagac atcgactatt ggcacttgct
    61441 gaatcaactc ttcatcagac ataagatcca aatcgatccc ttctaaaact ttggcagtat
    61501 gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc cctgtaatca
    61561 ttttcactga gatccctgct tgttggcatt cctttacagc tttgactgca ctttcttttg
    61621 gcggatcgat caatccggcg atccctgcaa aagttaattg ggttaaagtc tcatgcgtga
    61681 gttcctgttt cgaatctact tttttataag caaatcctaa aacacgctga ccttttctcg
    61741 caaattctgc agctgtctga ctccatgctt cttgttctgc agaagaaaga gttgatttag
    61801 caaataatac ttctggtgca cctttgacgt aaatcaccgc tcctttttcc tccgcatgca
    61861 tcgttgccat atatttgtat tcagaactga acggaatctt tgcttctatt tttttcaaag
    61921 gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg ccacttaaat
    61981 tttctgtttt ctgttcttct tttgttttta attcctgaca gtttttcata atggacaaca
    62041 cgcaggcttc ttcttttgca gctacatcca caactgtcat ttcatttttt gtcaatgttc
    62101 ctgtcttatc tgaacagatc actgtcatag aacctaatgt ttctaccgaa ggaagtcctt
    62161 tgatgatcgc cttttctttt gccatttcat gtactccaac ggatagaatc atggtcaaaa
    62221 cagctggcaa tccttcgggt accattgaca ccaccaaagc aatcgtagcc gaaagaagga
    62281 tatgccaatc cataccatat cgaaatgtcg taaaaataac taagaatagg ctcaaacaaa
    62341 gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga ggcgtcgttt
    62401 gttgttcgac cgattgcaat gcttgattga ttttaccgat ttctgtcgtg tttcctgttt
    62461 ctacaacgac accgactgca gaaccagatt gtacaagcgt tcctgaaaaa gccatatttt
    62521 tctggtcgcc gatcgattct tcttctgtca aaacggcatg acttttttca gatgcttctg
    62581 attctcccgt caaaatcgat tcttccacta ataaatcatg tgcttcgatt aatcggatat
    62641 ctgctggaac gacatctccg gcacgtaaga caatgacatc tccttttacc agttctttcg
    62701 cagggatttt ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc atcagctgct
    62761 tcaaaccatt caatgattct tccgcttttc tttcttgcca gtaactaacg agtccattga
    62821 cgatcactac gaataaaata atacttcctt caatatattc actagaagca aatttcaaaa
    62881 aagaggcaat gatcaacacg atcatcagca gatcagtaaa atgctttgct atttttttcc
    62941 atcgttttgt ctgctgcctt gcttttattt cattatatcc ttgatcagtc aggcgtttct
    63001 gtcgttcttc ttgcgacaag ccttctgttg atgtttgata gtttttcaat acatcttcta
    63061 ccgtcaatct gtaccagttc atcctatccc ctcctgttta attgatactg ttattatacg
    63121 cagggaagtt ttttcaggcg tatcgattat ttcagggggg tctgaaataa ttcgttttgc
    63181 aaaatgtatc tgggcctaac tgatatttct attgcttatt cttttatata caaaatttaa
    63241 gaatacaaac cagataacca ataaaaaagg aataaagcgt gtcgtgcttt cgaataacaa
    63301 gctgaaagca attgaagtaa aaaacaccaa caaagcaaga tctttatatc tttgtgtagt
    63361 gttccgtttc ttttctttaa taaattgaac atgtgcacac aagatcgaag accacacaaa
    63421 tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac tatcagctac
    63481 aaaaaaatgt agacaaagac cggcaaaaaa taacattcct gtaattaaaa taccgtgtaa
    63541 aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt cttttgctgt
    63601 ttgatataaa atcctgctag tactgtagac tgcactattt gctgaggaca aagaagcaac
    63661 caaaatcatt agattgacaa ttgctgctcc gccttggcaa tggatcactt gcagcatagt
    63721 cactaaagga ctttctttta aatctaaaca atgccatgga acaacaaata aaatcgacaa
    63781 aatcgttcct atatagaaaa agagaatacg ccaaggcaga cagttgattg cttttttcaa
    63841 attcttttgc ggatcacttg tctcgccaac ggttaagccg atcatttcaa ttccagcaaa
    63901 actgaataaa accataggaa atgagactac cattccagca aagccaaacg gtagtaattc
    63961 atcgatctgt ccaaaatttt ctttgattcc gatagactgg gtatgaaaat ctgagacact
    64021 caatcctata cccaataaga taaaaaggaa aatccccaag atcttgatga ttgagaaaaa
    64081 agattccaat tcaccaaacc acttgactgc aacaaggttg actgccatta agatcattag
    64141 tgcgctacct ccgcttatca aagctggtac atgtacaaac cagaaatgga tatacaatcc
    64201 tattgctgtt aattccgcaa gtccagctcc aagccagcaa agccaataag tccaattgac
    64261 aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa tcactgatag agttaaatgt
    64321 actatttgct aaaaataaat cacccatggc ccgcatcaaa ccataaatag ccaaaccgca
    64381 tatcaaataa acgagaataa cagctggccc tgctgatgca attgcctttg ctgaacctaa
    64441 aaataatcct gttccaattg cgccacctaa tgcgattaac tggatatttc gattagttag
    64501 agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca agcagcatgg
    64561 cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acagagtggt actacaaaca
    64621 aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct ttcatcgtca
    64681 gtccgacaga aaatccgatt gccgcaaata aaacaataac gacagtccaa actccaagat
    64741 tgctcttttt ctcttttttt ccaattctaa atggtcgatt caactttgca tgtgtataac
    64801 gcaatctcaa aactgcgatc cccatgatga catagacgat acagtagata acagtagttg
    64861 aatttacgag catcaaaaat gcttgattga cgccaggaat cagcaaataa aaaagtgcaa
    64921 agattgagat aatgacagac tgagtcaaaa taacggattt ggatacgtca aattgattcg
    64981 tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct gtcactgtct
    65041 ttgctggtcc tgaggcccaa gcagataatt ggactgctac accaatgaag acaagtaaac
    65101 taaacagatt cacaataaga tgtggcaaac caagaatttg gcaataaatc gaaatagatt
    65161 gcgcgatatt attcaattcc atttgtcctt ttggtacgac atttgctaca actaaagcat
    65221 tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa tactccgcga atatacgttt
    65281 taactggatt ttccaatcga gtgatataca cagaggacat ttcgatacca gtaaaaataa
    65341 acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt agtatctggt attaatttgt
    65401 cccatgaaaa actgcctaat gttccggttg gtacgatccc aactttgatc cacgcagcaa
    65461 accctaataa gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca ccgacttttc
    65521 cgccgatttt tgccatatca aatttcaaat tcaaaagagt gatgatccaa taaacgacta
    65581 aaatgacaac taatgtaaat ttgctgttca aaccaagcgg cacattatcg ataatattac
    65641 caaacaacgg agcgagtgct gaagcgacca tcaccatccc aggaaacatt tggacccata
    65701 acagccatga gacaacgaac ccccattttt ttcctagagc attcgatacc caaagttctg
    65761 gaccaccttt ttggggaaac attccagcaa attcaccaga gatcaatgag attggaaaag
    65821 cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt gctgctacat
    65881 ctggaatatt tcgtacactt gctacaaggg cgcaggtcat tccgataaag gtcatggtcg
    65941 tgatttgctt tgatgaacca gtatctttca tctttattcc ttctttcaat agcgacatat
    66001 gtttttgttt tcttttattt ttgttcaaga acagaaatga caaggtttca tcgattcagt
    66061 acaaaaccca gtatcaccga aacatccatt gaacgatctt agtgaactgg gtttgataca
    66121 tgaaacgaac gatccaactt atagaacgtt ttataaaaaa gaatatcttg gcgaggaata
    66181 tatgaaaaac agggggaaat acaattgttt tgatgaagca aataattgtc tgttctccat
    66241 atgtctcttg ttattctttc acacaaaaat cagttctatt ggatgatttc gtttagtttc
    66301 ttttccattg ctttggcgat tttttttgta taaaaattga aaattcgatc atcattcaaa
    66361 taaggagtca tgatagccgc acgtagcaga gtgattttct gttctttttt ccattcttct
    66421 ttcgttagac ccattctttc aacaaaacga atcggagaat caccgtaatc ttcttgtgta
    66481 aatgtagtat gagaagtaat aaaccgttca ccataaacat cgccatccat ataagaagac
    66541 cgatcgaaca ttttttcatt taattcattg atagcgttca aatctgtaca gccttgttct
    66601 ttgaataccc aattgaccat attaaaatca ggatgattca atggatagac ttcgattgtt
    66661 tttcctttaa cagtaaaagt cagatgatcg agaaattctc tgaatctttg agctgcctcg
    66721 attgaagccc caatcaattg accatatccg gtcacattca atggcaatac acgatgtgct
    66781 gtccagactg cggcggcggt tgctccagct tttgatcctt ccaaaatata agctcctaac
    66841 atatctggag cctttactga tttttcaaac acatatggcg cgaaataaga aatgatattt
    66901 ctcatatttt tatgtttgat tacgattcct ccagcagcat aagggacata gcccatctta
    66961 tgaggatcta tcgtgacaga atccgcttct gaaatagctc tgaacccttc atatacctct
    67021 ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt caaagaattc agctaaggaa
    67081 gcatacggca caaattctcc tgcttcgttt agaaacagtg aacgcgcata gccgccatat
    67141 gcggcatcaa catgtaaata gaaatagatc ccttcatctt ttaacctttc tcttaattgg
    67201 acgattttat ctacactgtc tacttgacct tcttctgtcg ttcctacgac agcaaccaca
    67261 cctagaattg ggattttttg tccagctaat tcacgaattg ttttttccaa agcattaata
    67321 tccattcgat aatcctcttg aactgggata gcaaccatct gatctaagcc gactccgcaa
    67381 atatctaacg ccttcatcca agaatagtgt tttgtttgag ggaccagcca tttccctaat
    67441 ctttggatat tttttccgct acgggaagaa gctgctttga cctcatccat ttcttcgtcc
    67501 gtaaagcttt cggtcatttc caaaatttct tcaacagata aattcaaaag ttcccactca
    67561 gacatttttt tcactttttc aggcagcact tccttgacag ctaaaggaat cgatttgatg
    67621 caacgggcgt accataatcc ttcaagatta gcaatagaac catctgctgc aatgtgtccc
    67681 catccgtctg tcatattgaa taatgaagca aaatcctgtc ctacttctgc ttccatttgc
    67741 gaagttgcca tagaagattc caatgcaaca ttatttgggt tccacagcat cgcatagttg
    67801 taagctaata aagcaggcat caatgtttca gcattcattt gtccccagta acggcctgct
    67861 gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa gacctctatc
    67921 atatggtcgc gggtggctag ataatctgga gtgagcttat ctccttcgct aattgcaggc
    67981 atatctgaag gaatataatt ttctctccat ccaagatgtt catctaccat cttgttcaac
    68041 aacattttat aaactggtcc attttcagct ttgtcgccta taaagacggc cttgatatcc
    68101 atatctttca tttttcttcc ctccatattt tgttggtcaa tgtcttgcag atacatgtgt
    68161 ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga aatttttttt tataacatca
    68221 aatccacgta gatttttaca aacaaaaata aataaagtcg gatttttctg ataattcaac
    68281 gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa aatagataac ctaaaaaaag
    68341 cgaacaaaca aataaaatga ctgtccttc
//
LOCUS       DARBEG010000003        67651 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000003 DARBEG010000000
VERSION     DARBEG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 67651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.003"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..562
                     /locus_tag="SCK20_000186"
     CDS             <1..562
                     /locus_tag="SCK20_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1144284.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="SCK20_000187"
     CDS             762..1076
                     /locus_tag="SCK20_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1144285.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="SCK20_000188"
     CDS             1097..1474
                     /locus_tag="SCK20_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1144286.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="SCK20_000189"
     CDS             1484..2257
                     /locus_tag="SCK20_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144287.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="SCK20_000190"
     CDS             2279..3682
                     /locus_tag="SCK20_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1144288.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="SCK20_000191"
     CDS             3864..5048
                     /locus_tag="SCK20_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1144289.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="SCK20_000192"
     CDS             5045..5335
                     /locus_tag="SCK20_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144290.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="SCK20_000193"
     CDS             5332..5553
                     /locus_tag="SCK20_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144291.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="SCK20_000194"
     CDS             5595..6374
                     /locus_tag="SCK20_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1144292.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="SCK20_000195"
     CDS             6441..6935
                     /locus_tag="SCK20_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1144293.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="SCK20_000196"
     CDS             6991..7629
                     /locus_tag="SCK20_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144294.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="SCK20_000197"
     CDS             7701..8096
                     /locus_tag="SCK20_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1144295.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="SCK20_000198"
     CDS             8080..10533
                     /locus_tag="SCK20_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1144296.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="SCK20_000199"
     CDS             10530..12557
                     /locus_tag="SCK20_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144297.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="SCK20_000200"
     CDS             12554..13576
                     /locus_tag="SCK20_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1144298.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="SCK20_000201"
     CDS             13593..14522
                     /locus_tag="SCK20_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1144299.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="SCK20_000202"
     CDS             14767..14883
                     /locus_tag="SCK20_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1144300.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="SCK20_000203"
     CDS             14899..15702
                     /locus_tag="SCK20_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1144301.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15937..16173
                     /locus_tag="SCK20_000204"
     CDS             15937..16173
                     /locus_tag="SCK20_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144302.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="SCK20_000205"
     CDS             16180..18132
                     /locus_tag="SCK20_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1144303.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="SCK20_000206"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="SCK20_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1144304.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="SCK20_000207"
     CDS             19063..20103
                     /locus_tag="SCK20_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1144305.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20201..20389
                     /locus_tag="SCK20_000208"
     CDS             20201..20389
                     /locus_tag="SCK20_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1144306.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(20447..20800)
                     /locus_tag="SCK20_000209"
     CDS             complement(20447..20800)
                     /locus_tag="SCK20_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144307.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            21331..21753
                     /locus_tag="SCK20_000210"
     CDS             21331..21753
                     /locus_tag="SCK20_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1144308.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21750..21980
                     /locus_tag="SCK20_000211"
     CDS             21750..21980
                     /locus_tag="SCK20_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144309.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            22478..22678
                     /locus_tag="SCK20_000212"
     CDS             22478..22678
                     /locus_tag="SCK20_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1144310.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22705..23898
                     /locus_tag="SCK20_000213"
     CDS             22705..23898
                     /locus_tag="SCK20_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1144311.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="SCK20_000214"
     CDS             complement(23966..25531)
                     /gene="guaA"
                     /locus_tag="SCK20_000214"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1144312.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25590..26513)
                     /locus_tag="SCK20_000215"
     CDS             complement(25590..26513)
                     /locus_tag="SCK20_000215"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1144313.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26709..27314
                     /locus_tag="SCK20_000216"
     CDS             26709..27314
                     /locus_tag="SCK20_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1144314.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27622..28638
                     /locus_tag="SCK20_000217"
     CDS             27622..28638
                     /locus_tag="SCK20_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1144315.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28779..30080
                     /locus_tag="SCK20_000218"
     CDS             28779..30080
                     /locus_tag="SCK20_000218"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1144316.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            30126..30788
                     /gene="deoC"
                     /locus_tag="SCK20_000219"
     CDS             30126..30788
                     /gene="deoC"
                     /locus_tag="SCK20_000219"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG1144317.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30809..31207
                     /locus_tag="SCK20_000220"
     CDS             30809..31207
                     /locus_tag="SCK20_000220"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG1144318.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            31273..32355
                     /locus_tag="SCK20_000221"
     CDS             31273..32355
                     /locus_tag="SCK20_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1144319.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32567..34129
                     /locus_tag="SCK20_000222"
     CDS             32567..34129
                     /locus_tag="SCK20_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144320.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            34122..35255
                     /locus_tag="SCK20_000223"
     CDS             34122..35255
                     /locus_tag="SCK20_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144321.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            35255..36211
                     /locus_tag="SCK20_000224"
     CDS             35255..36211
                     /locus_tag="SCK20_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144322.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            36310..37476
                     /gene="deoB"
                     /locus_tag="SCK20_000225"
     CDS             36310..37476
                     /gene="deoB"
                     /locus_tag="SCK20_000225"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG1144323.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            37500..38318
                     /locus_tag="SCK20_000226"
     CDS             37500..38318
                     /locus_tag="SCK20_000226"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1144324.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            38335..39042
                     /gene="deoD"
                     /locus_tag="SCK20_000227"
     CDS             38335..39042
                     /gene="deoD"
                     /locus_tag="SCK20_000227"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1144325.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="SCK20_000228"
     CDS             complement(39340..40026)
                     /gene="gpmA"
                     /locus_tag="SCK20_000228"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1144326.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(40198..40779)
                     /locus_tag="SCK20_000229"
     CDS             complement(40198..40779)
                     /locus_tag="SCK20_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1144327.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="SCK20_000230"
     CDS             complement(40912..41595)
                     /gene="rpiA"
                     /locus_tag="SCK20_000230"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG1144328.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41914..42327
                     /gene="rpsL"
                     /locus_tag="SCK20_000231"
     CDS             41914..42327
                     /gene="rpsL"
                     /locus_tag="SCK20_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG1144329.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            42411..42881
                     /gene="rpsG"
                     /locus_tag="SCK20_000232"
     CDS             42411..42881
                     /gene="rpsG"
                     /locus_tag="SCK20_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG1144330.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42975..45062
                     /gene="fusA"
                     /locus_tag="SCK20_000233"
     CDS             42975..45062
                     /gene="fusA"
                     /locus_tag="SCK20_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1144331.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            45234..46421
                     /gene="tuf"
                     /locus_tag="SCK20_000234"
     CDS             45234..46421
                     /gene="tuf"
                     /locus_tag="SCK20_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1144332.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46828..47136
                     /gene="rpsJ"
                     /locus_tag="SCK20_000235"
     CDS             46828..47136
                     /gene="rpsJ"
                     /locus_tag="SCK20_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG1144333.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            47167..47796
                     /gene="rplC"
                     /locus_tag="SCK20_000236"
     CDS             47167..47796
                     /gene="rplC"
                     /locus_tag="SCK20_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG1144334.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47824..48447
                     /gene="rplD"
                     /locus_tag="SCK20_000237"
     CDS             47824..48447
                     /gene="rplD"
                     /locus_tag="SCK20_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG1144335.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            48447..48737
                     /gene="rplW"
                     /locus_tag="SCK20_000238"
     CDS             48447..48737
                     /gene="rplW"
                     /locus_tag="SCK20_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG1144336.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48776..49609
                     /gene="rplB"
                     /locus_tag="SCK20_000239"
     CDS             48776..49609
                     /gene="rplB"
                     /locus_tag="SCK20_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG1144337.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49652..49930
                     /gene="rpsS"
                     /locus_tag="SCK20_000240"
     CDS             49652..49930
                     /gene="rpsS"
                     /locus_tag="SCK20_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG1144338.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49953..50300
                     /gene="rplV"
                     /locus_tag="SCK20_000241"
     CDS             49953..50300
                     /gene="rplV"
                     /locus_tag="SCK20_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG1144339.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            50314..50970
                     /gene="rpsC"
                     /locus_tag="SCK20_000242"
     CDS             50314..50970
                     /gene="rpsC"
                     /locus_tag="SCK20_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG1144340.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50973..51407
                     /gene="rplP"
                     /locus_tag="SCK20_000243"
     CDS             50973..51407
                     /gene="rplP"
                     /locus_tag="SCK20_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG1144341.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            51397..51585
                     /gene="rpmC"
                     /locus_tag="SCK20_000244"
     CDS             51397..51585
                     /gene="rpmC"
                     /locus_tag="SCK20_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG1144342.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51610..51876
                     /gene="rpsQ"
                     /locus_tag="SCK20_000245"
     CDS             51610..51876
                     /gene="rpsQ"
                     /locus_tag="SCK20_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG1144343.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51934..52302
                     /gene="rplN"
                     /locus_tag="SCK20_000246"
     CDS             51934..52302
                     /gene="rplN"
                     /locus_tag="SCK20_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG1144344.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            52339..52647
                     /gene="rplX"
                     /locus_tag="SCK20_000247"
     CDS             52339..52647
                     /gene="rplX"
                     /locus_tag="SCK20_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG1144345.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52675..53214
                     /gene="rplE"
                     /locus_tag="SCK20_000248"
     CDS             52675..53214
                     /gene="rplE"
                     /locus_tag="SCK20_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG1144346.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            53233..53418
                     /locus_tag="SCK20_000249"
     CDS             53233..53418
                     /locus_tag="SCK20_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG1144347.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            53454..53852
                     /gene="rpsH"
                     /locus_tag="SCK20_000250"
     CDS             53454..53852
                     /gene="rpsH"
                     /locus_tag="SCK20_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG1144348.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53884..54420
                     /gene="rplF"
                     /locus_tag="SCK20_000251"
     CDS             53884..54420
                     /gene="rplF"
                     /locus_tag="SCK20_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG1144349.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54581..54937
                     /gene="rplR"
                     /locus_tag="SCK20_000252"
     CDS             54581..54937
                     /gene="rplR"
                     /locus_tag="SCK20_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG1144350.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54958..55458
                     /gene="rpsE"
                     /locus_tag="SCK20_000253"
     CDS             54958..55458
                     /gene="rpsE"
                     /locus_tag="SCK20_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG1144351.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            55473..55652
                     /gene="rpmD"
                     /locus_tag="SCK20_000254"
     CDS             55473..55652
                     /gene="rpmD"
                     /locus_tag="SCK20_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG1144352.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55696..56136
                     /gene="rplO"
                     /locus_tag="SCK20_000255"
     CDS             55696..56136
                     /gene="rplO"
                     /locus_tag="SCK20_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG1144353.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            56136..57431
                     /gene="secY"
                     /locus_tag="SCK20_000256"
     CDS             56136..57431
                     /gene="secY"
                     /locus_tag="SCK20_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG1144354.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            57493..58140
                     /locus_tag="SCK20_000257"
     CDS             57493..58140
                     /locus_tag="SCK20_000257"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG1144355.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            58331..58549
                     /gene="infA"
                     /locus_tag="SCK20_000258"
     CDS             58331..58549
                     /gene="infA"
                     /locus_tag="SCK20_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG1144356.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58582..58698
                     /gene="rpmJ"
                     /locus_tag="SCK20_000259"
     CDS             58582..58698
                     /gene="rpmJ"
                     /locus_tag="SCK20_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG1144357.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58716..59081
                     /gene="rpsM"
                     /locus_tag="SCK20_000260"
     CDS             58716..59081
                     /gene="rpsM"
                     /locus_tag="SCK20_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG1144358.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            59109..59498
                     /gene="rpsK"
                     /locus_tag="SCK20_000261"
     CDS             59109..59498
                     /gene="rpsK"
                     /locus_tag="SCK20_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG1144359.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59578..60516
                     /locus_tag="SCK20_000262"
     CDS             59578..60516
                     /locus_tag="SCK20_000262"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG1144360.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60544..60924
                     /gene="rplQ"
                     /locus_tag="SCK20_000263"
     CDS             60544..60924
                     /gene="rplQ"
                     /locus_tag="SCK20_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG1144361.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            61196..62035
                     /locus_tag="SCK20_000264"
     CDS             61196..62035
                     /locus_tag="SCK20_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1144362.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            62011..62880
                     /locus_tag="SCK20_000265"
     CDS             62011..62880
                     /locus_tag="SCK20_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1144363.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62870..63667
                     /locus_tag="SCK20_000266"
     CDS             62870..63667
                     /locus_tag="SCK20_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1144364.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63870..64616
                     /gene="truA"
                     /locus_tag="SCK20_000267"
     CDS             63870..64616
                     /gene="truA"
                     /locus_tag="SCK20_000267"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1144365.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64949..67618)
                     /locus_tag="SCK20_000268"
     CDS             complement(64949..67618)
                     /locus_tag="SCK20_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG1144366.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        22219 a        12060 c        14068 g        19304 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgttt tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt
    20161 taatatagat ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat
    20221 attgtgtcct atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa
    20281 aaatttccct ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat
    20341 gaatataata acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg
    20401 aaactctcat cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat
    20461 ttcttgcttc attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa
    20521 agctatccat gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag
    20581 gtaagaaaaa ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg
    20641 tacttgggtg ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca
    20701 ttgttcccac ttgttcacga gtgagacccc gtttcattcg agcctcttta atcgctaaac
    20761 caacggctct aaaatcgtat ttatcttctt ttttacgcat actaaatcac ctctatacat
    20821 tttactgttc ctatataacc ataaacaggt atagaaaaac atgttatata gtttatgagt
    20881 tcctatagaa cactgtggaa tttattaatg cgatgaatcc aatttcaaat agaaaggtcg
    20941 ctgattttat ttacctatac aactttataa aatttagtca tactcctaga aagtagataa
    21001 aattggcgac ttaatggagg gaaaagtcgc catgagaaag ataatctgtc atctagttct
    21061 cttaattaag ttcttatcaa aaaaacttat ccacctgtac tggatagata tgaccatcaa
    21121 tgtggttttg cagattacat agatagaaaa atcaaagcat atgaacagag aaaacaatct
    21181 gtttatgtgc ttttttggtc acttaataaa tatcttttag gatttctctt catttatgca
    21241 acaaatcccc cgttcgtgtc ggggtttagt atgaagggag ataaagaagc acatgccact
    21301 ccctttcacg acagaaaggg ggtgaaaagc atgaaaccat cttcttttca agaaaccata
    21361 gaaaatcagt ttgactatat ctgcaaacaa gttattgaag atgaacgaaa agattacttc
    21421 aaacatctag acagactatc agcaaaagaa gtctcgtttt ctgatataga taactacctt
    21481 gtcaactctt tttctactgt tgatacgtac gtcactgatt tccaactatt taaattattt
    21541 ggattaagaa tcaatgttga aagtgattta ttaagtgaag ccctgcgtca tctttcagag
    21601 aaaaaacgga acattatttt acttcattac tttatggata tgagtgatgt ggagattgcg
    21661 gagttactaa atttgaatcg ttcgactgtt tatcgtcatc gaataagtgg actagccatg
    21721 attaaagaat tcatgaagga gtgtaaagaa tgaaaacagc ttatccaagg gttcccttcc
    21781 ccctcattgt aaaggcgaca gatggcgacg tggaagcaat taatcagatt gttaaacatt
    21841 atcgagggta tacctcaaaa cgttcattac gtcgcatgac agacgaatat ggaaattctc
    21901 atatggttat tgatgaaacc ctacgtggca gaatggaaac gaaactcatc tctaagattt
    21961 tatcgttcga aattaaatag tcccattttt tcgtggaagc gtgtcgaatc gtatccacgc
    22021 ttcccgattt gggaggtttg ttattccatc aaagcatatt gtgtgttcag taggttttga
    22081 taggctaaca agccaaacgt tctttgacaa ccaaataaag gcaatcgaat acgtttcggt
    22141 aagaaaagag ctgatgaact aacacgccat gacctacaaa attagtgagc gattcatgtt
    22201 agtaattcga aagtaatctt tagcaggatt gctgccatga ctttcttatc gtgataatga
    22261 tactcccgta tagccatagt ccgagcgtta gaagcgtcac aggcaatggg tacggctaca
    22321 tgagaaccat gcagaagtga aattcctgtg agctttgcta gagctgttcg attgctaaga
    22381 tattcttctc ttgtcagtgt attattataa aactatttga tataattata ggtgaatatt
    22441 ggagttttcc tagcctataa ttgggaaagg agtaataatg ataacggtca caaaaaagga
    22501 tttagttgag ctaggatatg gaacttcttt ttcagcagac atcataaaaa aagcaaaaga
    22561 attgatgatt acaaaagggc atatctacta tcaatctcga aagctagata gagtccctcg
    22621 tgaagctgtt gaagaaattc taggaattcg gttttcaaat gaaaacaggt ctgagtagtt
    22681 tttgcacagt aaggagtaaa aactatggtt aaagacccaa taaaaaaagc aaagaatgga
    22741 acctattatt ttcgtgcaaa tctaggttat gattctaaag ggaaaaaaat tcaaaaatat
    22801 cgtagtggat tcactactaa aaaagaagca agagaagaat attcgaaact gctattgatg
    22861 aaaccagaag aattaagtga gaataaggat aaaatgacgt ttaaacatta tatctttgaa
    22921 attttccttc catggtataa gacacaggta aagctcagaa catatgaaaa tcgcttacca
    22981 actattaaga agcatttctc ttattttgat aaaatggctg tttccgatat tgagcctatt
    23041 gatgtgcaaa attggcaact aaaattatca aagaagtgta agtcttctta tgtaagagct
    23101 gttcaaggac tattttcagt tgctatggat cgagcaattg tccttgggat aaccaccaca
    23161 aatccatcta agattattgg caatgtgaaa aagcaaaagt ctaaaattga attttggaca
    23221 aaagaagaat ttgaaaaagt aatctctcta atttacaaag aagattacta tcaacacttt
    23281 ttatttatct ctctgtggtt tttatttatg actggaatgc gaattggtga agctactgcc
    23341 attcaatggg aagatattga ttttgactca ggagtactaa ccatcaacaa aacactctat
    23401 tacaagaatc aatcgaatta tagatttgtt gaaccaaaaa caaaggcgag cgttcgtcat
    23461 attgtattag ataaatgcac attaacttat ttatccgaat ggaaggaagt acaacaaaat
    23521 ctaattcaga cagactttgt aatgagctat aacggtattc ctacccaaaa acatacacta
    23581 tcatatgcta ttgaacgcta tgctaagctg gctggtatcc atcgaatcag aatccatggg
    23641 ttacggcatt ctcatgcttc cttgttaatc agtatgggag aaaatccact tatcattaaa
    23701 gatcgtctag gacacgaaga tattgagacg actttaggaa cgtatggaca tctttatcct
    23761 aacagtaatt tcgaggtagc taacaaatta aatggagcca tttcctttaa agaagcaaaa
    23821 caaaattatg atacttcacc aaagaatcaa tttactgtag actacttgcg aaacaaagaa
    23881 atgaaaaaag tgcaataaaa gtgcagtaaa aagataagaa gagccagaaa cccttggtat
    23941 aagaggattt ctagctctcc tacaactatt cccactccac tgttgctggt ggtttagaag
    24001 tgatgtcata cacgatacgg ttgacgtggg ctacttcgtt tacgatacgt acagagattt
    24061 tttgcaagac ttcccatggg atacgggcaa agtcagcggt cattccgtcg attgaagtta
    24121 cagcacggat accgactgtg tagtcgtaag tacgtccgtc acccataaca ccgacagagc
    24181 ggatccctgg aagaacagtg aaatattgcc agatatcacg gtcaagtcca gcagctgcga
    24241 tttcttcacg caagatagca tcagattcgc gtacgatttc tagcttgtct tcagtgattt
    24301 cgccaagaac acgaatacca agaccaggac cagggaatgg ctgacgccaa acgattgaat
    24361 caggcatgcc taactgagtc cctaattcac ggacttcgtc tttgaataat gtgtttaatg
    24421 gttcgatcaa ttcaaattgc atatcttctg gcagtccacc tacattatgg tgagatttga
    24481 tcgtctgtgc tgtttctgta ccagattcga taacgtcagt atacaacgtt ccttgtgcta
    24541 ggaaagagac gccttcttca ccagcaagtt ttgttgcttc gtcatcaaaa acatatacga
    24601 attcattccc aatgatttta cgtttttgtt ctgggtcaga tacacctttc aacttgtcta
    24661 agaagcgatc tcttgcatcg actttgatga tgttcaagcc gaattttccg cctagacttt
    24721 ccattacttg ttccgcttcg cctttgcgta gcagaccgtg gtcaacaaag atagatgtca
    24781 attggtcacc gatcgctttt tgtaaaagaa ccccaacgac actagaatca acgccacctg
    24841 aaagaccaag caagactttt ttgtcaccaa cttgttcacg gattttttct acttgcatat
    24901 cgataaagtt atccattgtc cagttacctt cacagtgaca aacatcaaag gcaaagtggc
    24961 gcagcaattc gattccgtat tcggaatgac gtacttctgc atggaattgg ataccataaa
    25021 aattacgtgt tgtgtcttgg atagaagcaa tcggacaatc tttgcttgtt ccgactgttt
    25081 caaagccttc aggaacttta gttaccaagt caccatggct catccatact gtttgttgtt
    25141 ttggtgtttc tttaaacaag acagcttgat cgcttgttac ttctaacaag gctttcccat
    25201 attcgcggtt tttagcaggc tctacggaac cgccaagttt gtacgtgatc aattgcatac
    25261 catagcaaat ccctaataca ggaataccta aattgaagat ctcaggatcg atcccaaatg
    25321 cattctcatc atatacactg tttggtccgc cagaaagaat gattcccttt ggattcatcg
    25381 cttttacttc ttctgcagta atgcggtggc tcaataattc ggaaaagact ccaagatcac
    25441 gaatacgtct tgtgatcaat tggttatact gactaccgta gtctaatacg ataatctttt
    25501 caacgttcgt catatcggca acgtttgtca aatttattac ccctatccct ttaaatttta
    25561 tctcaaaata gtcaaactat tttaaaatgc taatattttc gcatgaaaat cgaatcgatg
    25621 atatgatttt cagttttatg gagaattata tcggctcttc ctcgtgtagg caagatatat
    25681 tcctgtaaat ttttcaaatt cacatttttc catacatctt ttgccatttt aaaggcttct
    25741 tcacgatctc cgatagcaaa acgataataa taattctctg gattttggaa agccgtatcc
    25801 agtaacgcac caaaacgatc caaataccat ttttcgatca gcacgggatc ggcatcgaca
    25861 tagacagaaa aatcaaaaaa gtcgctgacg tagatttgct gattcgcagg taattgaagc
    25921 gtattgatcc cctcaacgat caagatatca ggctgctgga tcaattcgta cttgtctgga
    25981 atgatatcat acacatcatg tgagtaaaca ggtactttta tctcttcctg accagatttt
    26041 acacggttca agaaatcgat cagcatctcc atatcataac tttcaggaaa tcctttacgg
    26101 tccatgattc cacgctcttt taatatacga tttggataaa gaaatccgtc agtcgtgatc
    26161 agctgaacgt ttcttcgttt gaaggtacga gacaaaatcg tctgcagcag tctggctgtt
    26221 gtactttttc caacggctac acttccggcg atcccgataa taaaaggcgg tacttctacg
    26281 tatttatgaa gaaataaacc tttactcaag aacaaagatt caaattcttt catataaaga
    26341 taaatcagat gagcaagagg tacatagata tctcgaacat cctgcatgga aatgcggtca
    26401 ttcaaacttt tgatattatc tagttcatct tgtgtcaaag gtgcttgccc attacgataa
    26461 aattctcgcc attcttctct tgccacacgg tagtagttca ttggatcatt catattagcc
    26521 tcctgcgttc tcccatcaat ggaactattt taacacaaaa taaacgcaaa ccccaccact
    26581 taagagagat tgccgatctt tttgtataat ctttataata gaaagaaacg gagtcttatt
    26641 cttgacttgc gcacggatac ataaaaggat agactgttgt catgaaagaa atgagaagga
    26701 tggaagacat gacaaatcat tactattctg aacgcccaga tacggcgcac gattttgaac
    26761 aatgggcttt tgagttaaaa ggcagaacct ttcaatttgt aacagattct ggtgtatttt
    26821 cacgtgaaac agtggattat ggttcacgtg tcctgattga tgctttcgat tgggaagagt
    26881 tacctgatga aggaaagatc ttagatgtgg gatgcgggta tggaccgatt gggttagctt
    26941 tggcatttgc gacacagcgg tttgttgaaa tggtcgacat taatgaacga gcggtaagtc
    27001 tagcgcaagg aaacgcaaag cgtaaccaaa tcgaacatgt tgatatccat caatcgaata
    27061 tctatgaagc agttcatgaa gaaacctatg cagctatcgt aagcaatccg ccgatccgtg
    27121 ctggaaaaaa agttgtacat gaaattttga caggcgcata tcctcggttg aagaagggcg
    27181 gtacactgac aattgtgatc cagaagaaac aaggagcgcc gagtgcgcaa aagaaaatgg
    27241 aagaaacatt tggtaatgca gaaatcgtca caaaagataa aggatattat atcatcaaaa
    27301 gtgtgaaaga ataactagct gaaaaatagt ggagcgacga aagagtggaa ctgggcgtgc
    27361 atgtacgggt acgtatcagt tctttttttg ttgagaagtt tttaaaaaca aagaactatt
    27421 ttttcttcaa aaagaaatag taggaacaga gtcgttgttt tttacgataa aataaataag
    27481 atacagaact tgtatagaag gctgccaagt cggtagataa aggaggtttt tatcaaaaaa
    27541 catactttcc ttttttctct attcttttct tgggggtttt ggtacactga tagggaattt
    27601 agatagaagg gagccaatag gatgaagctg accatcagag atatcgcaga aatggcaggc
    27661 gtatcagtaa caactgtctc acagattttg aataacaaag gaagcagatt tagtgataag
    27721 acaagaaaaa aagtgttgtc gatcgtgaat gaacatcatt ataagcccga ttatttcgct
    27781 tcaaatgtga tcaatcgaca ttcaaagacc atcggaatga tcgtccctga tgtcactgat
    27841 ttcttcttct ccaaactgat cgaaggtgta gagagctatt tgaatccttt gggctatgtg
    27901 atcatgttat gcaattctcg tcatagccaa gaaaatgaga tcaagtattt acaagagctt
    27961 tcccaccgct cagtggacgg aatcttgctg gccacaccaa atattttacc tgagaaatat
    28021 gcattagaca gcggttttta tcaaaaaatg ccaatcattt taatcgatcg cggattgaat
    28081 cgtcgggata acggacggct tatcgtaaag gaatatgagg gcgcctatca agctgtttcg
    28141 ctgtttattg aaaatggcca tacgaagatc ggcatgctga aggaaaccac tggatactat
    28201 caattagaag aacgtttcaa cggatatcgt catgcgctga aagatcatgg cctgcctttt
    28261 aatgggaaat ttgttgaaca tggagagttg actgtccaag gtggatatac tgcatcaaaa
    28321 aaattactca aacataaaga tatcacggct attttttgcg ggaatgatgc catggcgatc
    28381 ggatgctatc aggcgatcga tgaattgggc aaaaaaatcc cagaagatat ctcagtcata
    28441 ggattcgatg gattaaagct gtcagaatac atgattccgc aattgacgac agtgcagcag
    28501 ccaagttttg atattggatt ttatgctgca cgtttcctga tcgatacgat cgaatttcct
    28561 caaagaaaag ttccgaacaa agtgttcgaa acaaaattaa ttattcgtga gagcgtaaaa
    28621 gtattgacaa agggctaact cggcggtata ttgcttatgt aagcatttac aaaggctagt
    28681 tagtgctttt tttattttta gtaaaccagt ttactaaagt ggtttacctt tcctaaaaat
    28741 aatgtataat accgaatgaa gacaaggagg atcaggcaat gcgaatggtg gatctgattg
    28801 aaaaaaagag ggatggaaac gagctgtcga aagaggaaat cgagtacatc gtcacgaact
    28861 atacgaatgg aaagattcca gattatcaag tcagtgcatt attgatggca attttttatc
    28921 aagacatgac aaatgaagaa atcacgaact taacattggc gattgctaat tctggtgatg
    28981 tcatcgattt gagttctctt gaaggaataa aggtagataa gcattcgaca ggtggcgttg
    29041 gtgacacgac cacattgatt ttagcccccc tagtagctag tgtcggagtg acagtggcta
    29101 aaatgtctgg acgcggtcta ggttacacag gtggtacact agacaagcta gaagcaattc
    29161 ccggatttca gatcgagctt tcggatgaag aatttgtacg cattgttaat gaaagtaaag
    29221 tagcggtaat tggtcaatca ggaaacttgg caccggcgga caaaaaattg tatgccttgc
    29281 gtgacgtcac agcgaccgtt gattctcttc cgctgattgc cagttcgatt atgagtaaaa
    29341 aaatcgcggc aggagcagat gcaatcgttt tggatgtgac cactggagac ggtgctttca
    29401 tgaagaacat cgaagatgca agacgtctgg caaagaccat gacgagcatt gggaaactgg
    29461 ctaaccgaga aacagtggcg gttatctcag atatgtcaga acctcttggc gaagctatcg
    29521 gaaatagtct agaagttgtc gaggcaatcg aaacattaca aggaaatggt ccagaagatc
    29581 tagtagaaat gtgctacgcc ttaggaagcc aaatggttgt tcttgctggc aaagctaaga
    29641 caaccgatga agcaagaacg ttgcttcaag aagctctgga atcaggtaaa gcgttagcaa
    29701 aattcaaaga aatgatccaa aatcaagggg gagacccagc gatcgtggaa catccagaac
    29761 gaattttgac tgcacgctac acaatggagc ttcctgctaa acaaagcgga gttgtgtcaa
    29821 aaatcgtggc aaatgaatta ggaattgctg caatgatgct aggtgctggg cgtaaaacca
    29881 aagaagaaga tattgatcac gctgttggat tgaaactgca taaaaaaatt ggtgacactg
    29941 tgacaaaagg tgagtcacta ttgacgatct atagtaacga cgaagaaatc acttctgtca
    30001 ttgaattatt atataaaaat attgaaattg gtgaatcggc aatgaagcca acgttgattc
    30061 atgacatcat cacagaataa aggaaaaaag cggactttac cgttttaaag aaaaggggaa
    30121 aagaaatgga actaaaccga atgatcgacc atactatttt gaaagcagac gcatctaaag
    30181 aagctgtgat gcaaattatc gaagaagcaa aaaaatacca tttttattct gtctgcatca
    30241 atccagcatg ggttgcgcta gcgaaagaac aattacaagg tgagccagta gccgtatgta
    30301 cagtaatcgg gtttcctcta ggagcaaaca cacccgagac aaaagcattt gaaacaacag
    30361 atgccatcaa taacggtgca gatgaagtcg atatggtcat caatatcggt gctttgaaat
    30421 caaaggatga tcaacaagtc caaaaagata tcgaagcagt tgttgaagca gcaaaagatc
    30481 gtgcgcttgt caaagttatt attgaaacat cgttattaga tagagaagaa atcatccgag
    30541 cttgcgaaat agcaaaagct gcaggcgctg attttgtcaa aacatcaact ggtttctcaa
    30601 caggcggagc aaaggtagag gatgtccgtt tgatgcgtga aactgtagga ccagaaatgg
    30661 gtgtcaaagc ttccggcggt atccataacg aagaagaagc aatggcaatg atcgaagctg
    30721 gtgctacaag aatcggaaca agcgcaggag tagcaatcgt atctggttca actggtgaag
    30781 gctattaatt ttttcaaagg agaagctcat ggcagaactc aaacaagaat ggatcaacac
    30841 agctattgaa gcgttagata aagcctatgt tccttattcc cattttccgg taggcgcttg
    30901 tctcgtaacg gaatcaggca agatttatca aggcatcaac attgagaatg catcatttgg
    30961 attaacgaat tgtgccgagc gcacagcttt tttcaaagca gtatctgaag gagaaaaaac
    31021 atttacgcat ctagtcgtag cggggcacac ccccgatcct atctctcctt gtggtgcatg
    31081 tcgacaagta atggcagaat tctgttcccc tgatatgccg gttacgctag taggagacaa
    31141 tggtgtgacg aaggcaacga ccgttcgtgc actgctgcct tatgcattta ctgaaaaaga
    31201 tctataactc atgttcattc agtcagctga ctgttgaaaa ataaaacaat tattttagga
    31261 ggctttacag aaatgaaaaa agcaaaatta tttggtttag gtgctgtcgc actagcagca
    31321 gggttatttt taggtgcatg cggcaacaac ggctctactg attcatccgg tggcaaaagc
    31381 tcatcagaca caacaactgc agcattgatc acagacacag gtggtgtaga cgaccgttca
    31441 ttcaaccagt cagcttggga aggactagaa aaatggggga aagatcaagg actttcacga
    31501 ggaaatgatg gcttccaata tttccaatct tcaaatgaat cagattacat cccaaatatt
    31561 gaccaagcat tgaatgctgg attcaaaacg atttttggta ttggttataa actaaaacca
    31621 gcgatccaag aacaagcaac aaacaataca ggtacgaact ttgttattat cgatgatgtc
    31681 atcgatggat tagataacgt tgtatctgca actttcaaag acaacgaagc ttcttatctt
    31741 gcaggtgttg cagcagctta tacgactgaa acaaacgtag tcggctttat cggcggtgta
    31801 aaaggtgaag taatcgatcg ttttgatgct ggattcaaag ccggtgttga tgcaggtgcg
    31861 aaagaattag gaaaagaaat caaagtattg aatcaatatg ctggtgactt ctctgctcca
    31921 gataaaggac gttcaattgc tcaaggtatg tatgcacaaa acgcagatat catcttccat
    31981 gcttcaggcg gtacaggtaa cggtgtattc caagaagcaa aatcattgaa cgaatcaggc
    32041 gacaaaaaag tttgggttat cggtgttgac cgcgaccaat cagacgaagg cgaatacaca
    32101 ttaaacggcg aaaagaaaaa cttcacattg acatctacac tgaaagcagt tggaacagta
    32161 gtagaagatt tggcacaaaa atcagcggac ggtaaattcc ctggcggcga acacactgtt
    32221 tatggattga aagaagacgg tgttggtcta acagaaggac aactttctga cgaagcgaaa
    32281 aaagcagttg atgaagcaaa agaaaaaatc atttcaggtg acgtaaaagt tccagaaact
    32341 cctgaagaaa actaaaacca tttctatagc cttgttagac acatgtttga caaggctatg
    32401 gtttgattag aacaagaatt gttcatttat ccaagctgcc ttgtgacagt ttggataagg
    32461 gaacaactcc ctgaagtaat gtttgtttca aaagaagaac gctcactaag cgtgaatctt
    32521 agtcaatcgt tgccctagat agaaatcgtt aggatgtgaa aaaaaggtgg ttcaagaaaa
    32581 tattgtcata gaaatgcaga atattaccaa agaatttgga acattcaagg caaatgacaa
    32641 catcaattta caagtaaagg caggagaaat tcatgcgttg cttggagaaa acggtgctgg
    32701 caaatctaca ttgatgaacg tgctttccgg attattagag ccgacatcag ggaaaatttt
    32761 gatgcgtggg aaagaagtaa agatcacaag tccgaccaaa gccaaccaat tagggattgg
    32821 gatggtccat cagcacttta tgcttgttga tgcctttact gtaacagaaa acatcgtgtt
    32881 gggaagcgag cctagtcgcg cagggatgct tgaccataaa aaagcacgaa aagagatcca
    32941 aaaagtttct gaacagtatg gattgtcagt caacccagat gcttacgttc gtgatatttc
    33001 agttggaatg gaacaacggg tagaaatttt aaaaacactt taccgaggag cagatgtact
    33061 gatttttgat gagccgacag ctgtattgac ccctcaagaa attgatgaat taatcgtgat
    33121 catgaaggaa ttagtcaaag aaggcaagtc gatcattttg attacgcata agttagatga
    33181 aatcaaagca gtagctgacc gctgtacagt tatccgccgt ggaaaaggaa tcggtacagt
    33241 caacgttaaa gacgtaacct cacaacaatt agccgatatg atggttggaa gaacggtttc
    33301 attcaaaacg atgaaaaaag aagcgaagcc tcaagaagtc gttttgtcta ttgaaaatct
    33361 agtggtgaaa gaaaatcgtg gattagaagc cgtgaaaaac ttgaacttag aggttcgtgc
    33421 tggcgaagta cttggtatcg ctggaatcga tggaaacggg cagtcggagt tgatccaagc
    33481 tttgactggt ttgcgaaagg cagaaagcgg acatatcaag ctaaaagggg aagacatcac
    33541 taataaaaaa ccccgaaaaa tcactgaaca tggtgtagga cacgtgccag aagaccgtca
    33601 taaatacggg ttagtcctag atatgacatt atctgaaaat atcgcactac aaacctatca
    33661 tcaaaaacct tacagtaaaa acggcatgct gaattattca gtaatgaatg aacatgccag
    33721 agaattgatc gaagaatatg atgttcgaac aacgaatgaa cttgttcccg caaaagcttt
    33781 atcaggcgga aatcagcaaa aagcaattat cgctcgtgaa gtcgaccgag atcctgatct
    33841 gttgattgtt gcaaatccaa ctcgtggact ggatgtaggt gcgatcgaat ttattcataa
    33901 acgtctgatc gaacaaaggg acaaatacaa agcagtgtta ctgatcagtt tcgaattaga
    33961 agaaatttta aatgtttcgg atcgtattgc tgttatccat gaaggagaaa ttgtcggtat
    34021 cgttgatccg aaagaaacat ctgaaaatga attaggtcta ttgatggcgg gctatacctt
    34081 agaagaagca cgaaaagagt tagaaaaggg ggctggcgca catgaatgat cgcagcgaaa
    34141 aattccgtaa tttattagtg cctgtacttt cagttgtttt aggattgatc ttaggcgcga
    34201 tattgatggc tgcttttggt tttcagccca tccaaggtta ttcttctatg ctagatgcag
    34261 cccttggttc gcaaagaggg ataggtgaaa cgttaaggca agcaacgccg ttgattttta
    34321 cagcattagg tttttctgta gctaactcag ctgggttttt caatattggg ctttctggac
    34381 aagcactttg cgggtggatc gtcagtgtct gggtggcact tgcgtttcca gatctgccaa
    34441 aagttgtttt gcttccgtta tgcgtgatct taggtgcctt agctggtgcg atggcagcgg
    34501 ctgttccagg tttacttcga gcattttttg gaacaagtga agtgatcgta acaatcatga
    34561 tgaactatat cttattatac gtaagcacat ttgctttaca tgaagtgatg cctgaaagct
    34621 atcgctcaag tttggactct tccaatatga tcagtcaaaa tgcctcactg cggttaccat
    34681 ggttaacaca agcttttggc ggctctcgcg taaactcagg attattttta gcaatcatcg
    34741 cgcttatttt cgtttggctg ctgatgaaga aaacgacttt aggatttgaa atccgttctg
    34801 ttggattgaa tccttttgct tctgagtatg caggaatgag tagtaaacgt acgatcgttt
    34861 tgtcgatggt catttctggc gctttggcag ggcttggcgg tgtggttgaa ggattaggaa
    34921 cgtatcaaaa cttctttgtt caaacgactt cgctatctat tggatttgac ggaatggctg
    34981 tttccttgct tggttctgga tcagcaatcg gtattctttt atcagcgatc ttgttcagta
    35041 ttttgaagat cggtggactt ggcatgcaga caggagcagg tgttccgttt gagatcgtca
    35101 atgtatcgat tgctttgatt atcttctttg tcgggatcaa cttcctgatt cgtttcgtca
    35161 tggctaaagt cttccaaggg aaaaaacaag aagagatcgt tgcgacaata gaatcgaaac
    35221 caacgaatca aaaccatgaa gggggcgaac tataatgtcg actatcgaat taattgcctt
    35281 gatccttact tctacactgg tttattcaac accgctaatt ctaacgtcac taggcggaac
    35341 tttttctgaa cgaagcggga tcgtaaacgt agggctagag ggaatcatgg tcatgggtgc
    35401 ttttagttcc atcgttttca acttgacgat gagtccatca cttggttcta tgacaccatg
    35461 gatcgctatt ttagtgggtg gcgtcgtagg tatcctattt tctctgattc acgcagtagc
    35521 aacagtcagt ttgcgtgctg accatattat cagcgggaca gtaattaatc tgatggcacc
    35581 tgctttaggt gttttcttga tcaaagcatg gtatggtaaa ggtcaaactg ataacatcac
    35641 gcagaatctg gggtattttt ctttcccagg attagcagat attccggtca ttggaccaat
    35701 tttctttaaa aatacattct tacctgctta ttgtgctatt ttgattgctg tcctagcttg
    35761 ggtgatcatc ttcaagacga aattcggctt gcgcctccgt tcagttggtg aaaatcctca
    35821 agctgccgac acattaggta tcaatgtata tgctatgcgt tattcaggcg tactgatttc
    35881 tggcttgtta ggcggtatgg gcggtgctgt ttttgctcag tcgatttccg gtaacttttc
    35941 agctggtacg atcgttggac aagggttcat ctccatggct gctatgatct ttggtaagtg
    36001 gaatccttta ggggcaatgg gtgcctcatt gttctttggt tttgctcaaa gcttgagtat
    36061 cattggcgca caattaccag ttatttcttc gattccgcca gtattgctgc aaattgctcc
    36121 atatttgctg acaatcattg tgctggttgt tttcttaggg aaagcttctg gtccaaaagc
    36181 aaacgggaag aactatatca aatcaaaata atcttttagc gaataaaaag ggtgggttac
    36241 gggaagtgtt tttcgtctcg ccctttttgt tttaaaggca ataaaaataa aaagaaagaa
    36301 ggaataatca tgtttaaacg cgtacatctg atcgtcatgg attcagtagg aattggagaa
    36361 gcaccagatg ccgataagtt cggtgatgtt ggttctgata cattaggaca cattgcgaaa
    36421 gaagcaggat tgaacgttcc acatttagag cagcttggtt taggaacgat ccgtcctctt
    36481 gaaggtgtgg aatcagtgaa agatcatcaa ggctatgcga cgaaattaga agaagtttct
    36541 gtagggaaag atacgatgac aggccactgg gaaatcatgg gattgaatat caaaactcca
    36601 tttcgtgtgt ttcctaatgg attccctgat gatctgatca aacaaatcga agaattttct
    36661 ggccgtaaag tcgtatgtaa caaaccttat agcggaacag ctgttattga cgattacggt
    36721 gaacaccaaa tgaaaacagg cgatttaatt gtatatactt ctgccgatcc tgtccttcag
    36781 atcgcggcac atgaagaaat cattccttta gaagaattat atcggatttg ccaattcgtt
    36841 cgtgatataa caaaagatga tccgtatatg attggccgaa tcattgcacg tccgtacgtg
    36901 ggtgaaccag gagacttcac acgaacaagc aatcgccatg attatgcgtt gaatccattc
    36961 ggacacacag tccttgattc tttgaaagaa gcaggaaagg acgtgattgc tgttggaaag
    37021 atcaacgata ttttcaacgg acaaggaatc actgaatctg tccgcacgaa aagcaatatg
    37081 gatggcgttg accagctgct gaaagttatg aaaaacgatt tttcaggaat cagctttacc
    37141 aatttagtag attttgatgc attatacggt catcgtcggg atgtagtcgg ctatgcgcat
    37201 gcgattgaag aatttgactt acgaattcca gaattgcttg aagcgatgga agaagacgac
    37261 ttgctaatga ttacagccga tcacggaaac gatccgacgt ttactggtac cgatcatacg
    37321 cgagaatatg taccgttatt ggtttacagt aaaaaaatga aagaacaagg ggcattgcct
    37381 caaggatatt attcagatat ttcagcaacg attgctgaga actttagtgt ttcttcgact
    37441 gaaaatggcg aaagcttctt gaaattatta aaataatctt tttaaatagg agaaagcaaa
    37501 tgaccaagtt aagcgagatg ttacaagaaa cgacccagtt tatccttgat aaaggagtaa
    37561 aagaaattga ttttggtttg attctaggtt caggattagg tgaattagca gatgaaatcg
    37621 aagatgctgt aatgatttct tatgatgaga tcccgttctt tcctgtttcg acagtagttg
    37681 ggcatgcagg aaagctagtt tatgggtctc tggcaggcaa gaaagtgtta gccatgcaag
    37741 ggagattcca ctactatgaa ggtcattcca tgcaaacagt gacctatcca gtacgtgtga
    37801 tggccggact aggggcccat tcagtcattg tgactaatgc atgtggtggt gtcaacgaat
    37861 catttgtacc aggagatttg atgctgatta ccgatcatat caattttact ggtgaaaatc
    37921 ctttgatcgg tccgaatgaa gatgcaatgg gccctagatt tccagatatg agtcaagctt
    37981 atacgcctga ctatcaagca acagcaaaag cagcagcaga aaaactaggt ttggatctta
    38041 aagaaggcgt atatatgggc tattctggac caacttatga aacgcctgcg gagatccgaa
    38101 tgtctcggac aataggagcc gatgcagtag ggatgtcgac tgttccagaa gtcatcgtag
    38161 cagctcacag cggattaaaa gtgttaggaa tctcttgtat taccaactta gcagcaggta
    38221 tgcaagcgaa tctgagccat gaagaagttg tagaaacaac tcagagagtc aaacaatctt
    38281 ttaaagcatt ggttaaagaa acattgacat tattgtaata ggaggaacaa taaaatgagt
    38341 gtgcatattg aagcgaaaga aggcgagatc gccgataaga ttttacttcc gggagatcct
    38401 ttgcgagcta aatatattgc ggagactttt ttggaagatc cagtttgtta caatcaggta
    38461 cgcggtatgt taggctttac cggcaaatat aaaggagaac gtatttctgt tcaaggaaca
    38521 ggaatgggaa tgccatcagc caccatctat gcccatgaat tgatccagtc gtatggtgta
    38581 aagaaattga ttcgagtagg tacatgcggt gcattgtcaa aagacgtcca tgttcgtgat
    38641 ttagtactag cacaaggtgc agcgaccagt tcttcgatga tcgaaaaaaa tttccaagcc
    38701 tttcattttc ctccaatcag tgatttcaat ctattactga aagcatatga gatagctaaa
    38761 gaaaagggct atacggttca tgtaggaaat gttctttcgg aagattcttt ctacaaagat
    38821 gatttgacag agacgttcca attagctgaa cttggcgttt taggagtgga aatggaagca
    38881 gcggctcttt attacttagg cgcgaaatac catgtgcaga cattaagttt gatgaccgtt
    38941 agcgaccatt tgatcaccgg tgaagaaaca acagctgcag aacgtcaatc tacgttcaat
    39001 gaaatgatcg aagtaggatt ggaaacagcg atagcagaat aagaaggaaa tgaaaaagct
    39061 gccctgcatt aagtaataag aacatccaag caaaaatagc tcctcatgtt tttgacttgg
    39121 ctggacttat tattgcaaat gtaagctttt gaacaccgtt taccaagagg ttgtgaatct
    39181 gccgtttaac tctgagtcac aaggaacaat ttcaaaaaac agcttttcat gtttgatgaa
    39241 tattgtgact tgtgaccgag aagttggcag gagaaaaccg tttatacgaa aaaagggact
    39301 gtgacaaatc gtgtcacagt ccctttttta tagtgttttt tataggtagt attttttagc
    39361 aactgttaag tcatcattta attcataaac aagtggttga cctgttggaa tttcaagatc
    39421 catgatgtct tcgtcagaga taccttcgat atgtttagct agggcgcgta atgagttacc
    39481 atgtgcagct actaaaacag ttttgttgtc tagtaatgct ggagcaattt catcttgcca
    39541 gaatggcaaa gcgcgttcta atgtcacttt caagttttct ccaccaggaa tatcgcgttt
    39601 gtctaacatt gcgtaacgac ggtcatttgc tgcagaacct tcgtcagttt cttccattaa
    39661 tggaggaaga gtatcgtaag aacgacgcca gatgtgcact tgttcgtcgc cgtatttttc
    39721 agctgtttct tttttgttca aaccttgtaa tttaccatag tgacgttcgt ttaagcgcca
    39781 tgatttgatt tgtggtaccc aaagttgatc tgaatattct aagatcaagt tacatgtttt
    39841 gattgcacga gttaaaacag aagtgtaagc aacatcaaat tcgattccag cttctttgat
    39901 tttgcgtccg ccttcttttg cttcttcgat tccttctggt gctagattta catcagccca
    39961 accagtaaat tggttcaatg cattccattc actaagaccg tgacgagaaa aaactaattt
    40021 tggcatgttt agctctcctt ttaagatagg tttatatttc tgaaattttc agttctgttt
    40081 tattgtacaa ttttttggaa gagatttcca tcgaaaaatt agataagatt ctatgagaaa
    40141 acaagtagag aatataaaaa aacgaacagt gcccacgcaa ctgttcgttt ttaggtatta
    40201 gatgcataat tgctcgattt ctttttcagg taaattgata tatttataac atgttccaac
    40261 tgaaacaccg cattttgaag aaataaattg gatcgtttct tttttttcat aatagagacg
    40321 tcggattttt ttgatcgttt ttgcatcgat ttttggtctt ccgccgattt ttcctttttt
    40381 acgagcttca tctaatccaa ctaatgtacg ttctttgatc aatgcgcact ccatgtttgc
    40441 taggttttcc attaattgga aatagatttt tcccatttcg cgcgtatcga ttccttcatc
    40501 aagactaatc aagtgcagcc cgcgatcttt gaataattgc gtgagttctg ttaattgccg
    40561 cgtagatttg cctaatcgat gaagttggca aatgactagt gcatcgcctt gttgcatgtc
    40621 agcgatcact gactccaaat gaacagcagt agaatcattt tgttccactt catatgtctc
    40681 atggaaaatt tcttgacaac caaagtcaga taattgatca acctgagttg ttaaatcaac
    40741 gtcatttatg gttgtgcgtg cataaccaat aattttcatg gtgattcctt tctagcagtg
    40801 attttctaac tggatataac agtaaaaatg tagcataaag tacgtcaaaa agatagtttt
    40861 ttcgcaaatt ttccgctgtc tttttttatt ggatgatcaa gcagtttttc tttatttacg
    40921 acggattgcg tcaagaatct caacgccttt ttctgagccg acaactactt tagaaaccat
    40981 gtttaggaaa agcccatgtt ctactacccc aacaagttca tctaaatagg tagctaagct
    41041 ttctggctgt tcgatgactt ctagatgcag atcaatgatg taatgaccac catctgtggt
    41101 taagacttct ccgttttcgt ctgtacgcaa gacgggttgg aatctttttt cttcaaaaat
    41161 atgaagtagc tgttgggagc cataagggat cacttcaact gggagtggga acttcccaag
    41221 tttatcgact aatttagaag aatcaacgat ccaaatcgtt tcttttgagt acgtagcgac
    41281 tattttctcg aaaagcaaag cagcgccgcc gcctttgatc ccttgaaaat cagcactgat
    41341 ttcatcagca ccgtctattg tcaaatcgac tacggggact tcatcaatac tctttaacgg
    41401 tattcctaat ttttccgctt gttcttttgt cgcatttgaa gtagtaactc ctgtgatacg
    41461 taaaccttct tcacgcatac ggcgaccaat ctcttctacc atataatagg cggttgaacc
    41521 ggtcccgaga cctacgatca tcccgtcctt tacgaatttc gcggcttcaa tgccagctaa
    41581 ttgttttaaa ttcatctttg cgtccacctt tttttagttt gagcatcgtg ctttcctcac
    41641 tattttagcg tgatttacgg taaaaagata ccattctttt ttattccaat aaagcttttt
    41701 caaaaaaaat tgcatctctt gttgacatgt tttttcattc gtgatattct aacaaaggtg
    41761 ctttatctac aggtttctgt gaagaaacgt gctgactgtt tatcaaagca tatttgataa
    41821 ggatgcagac attgcattta ttttttatca caaaaaaaga accacctgga tgtgtggaac
    41881 tagacgcaaa tcgtgggaag gaggaaacaa ggaatgccta caattaacca attagtacgt
    41941 aaacctcgta aatcaaaggt ggaaaaatct aattcacctg ctttgaacaa aggatataac
    42001 agctttaaga aaactcaaac taacgtaaac tcacctcaaa aacgtggggt agcaactcgt
    42061 gtgggtacaa tgacacctaa aaaaccgaac tcagctttac gtaaatatgc acgtgttcgt
    42121 ttgtcaaact taattgaagt tactgcttat atcccaggga ttggtcataa cttacaagaa
    42181 cacagtgttg tattattacg cggtggtcgt gtaaaagact taccaggggt acgttaccat
    42241 atcgttcgcg gtgctttaga tactgctggt gttaacgacc gtaaacaaag ccgttctaaa
    42301 tacggaacta aaaaacctaa agcttaattt taattattaa acaaatgaaa cttgcttcta
    42361 ctatatagaa gtaattacta tgaacaatat ttcggaagga ggagttacgg atgccacgta
    42421 aaggtcctgt tgcaaaacgt gatgttttac cagatcctat ttataactca aaattagtaa
    42481 ctcgcttgat caaccgtgta atggttgacg gaaaacgcgg gattgcagct aacattatct
    42541 ataatgcctt tgacttgatc aaagaatcta caggtaacga tccattagaa gtgtttgaac
    42601 aagcaatgaa aaacgttcaa cctgttcttg aagttaaagc acgtcgtgtt gggggttcaa
    42661 actaccaagt accagttgaa gttcgccctg aacgccgcgt tactctaggt cttcgttggg
    42721 tagtaaacta cgctcgcttg cgcggtgaac acacaatgga agaacgctta gcaaaagaaa
    42781 tcatggatgc tgcaaacaac acaggtgctt ctgttaaaaa acgtgaagac actcataaaa
    42841 tggctgatgc caaccgtgcg tttgctcact atcgctggta agattctctc tgtttggtcg
    42901 gcttttataa gtcgacctca gatagtgtcc ttatttttag caactagata gtaaagagag
    42961 gagaaacaag gagaatgacc agagaatttt ctttacaaaa cactcgtaat atcggtatca
    43021 tggcccacat cgatgctggt aaaacgactg ctactgaacg tatcttgtac tatactggta
    43081 aaatccataa aattggtgaa actcatgaag gagcttcaca aatggactgg atggaacaag
    43141 aacaagaacg tggtattacg atcacttctg ctgctactac agcacaatgg aagggtcacc
    43201 gaatcaacat catcgacaca ccaggacacg tagacttcac agttgaagtt gaacgttcat
    43261 tgcgtgtact tgatggtgct gtaactgttc ttgatgcaca atcaggtgtt gaacctcaaa
    43321 ctgaaacagt ttggcgtcaa gcaacaactt atggtgtacc tcgtatcgta tttgcaaaca
    43381 aaatggacaa gatcggtgct gacttcttat attcagtatc aactttacat gaccgtttgc
    43441 aagcaaacgc tcacccaatc caattaccaa ttggtgcgga agacgacttc acaggaatta
    43501 tcgaccttgt aacaatgaag gctgaaatgt atacaaacga tcttggaaca gagatcgaag
    43561 aaacagaaat tcctgaagaa taccgcgaat tagctgaaga atggcgcgaa aaattggttg
    43621 aagctgtagc tgaaactgat gaagaactta ctttgaaata cttagaaggt gaagaaatca
    43681 ctgaagctga gttaaaagaa ggtatccgcc gcgcaacagt taacgtagaa ttctacccag
    43741 tactatgtgg ttcagcattt aagaacaaag gggttcaatt actattggac gctgttcttg
    43801 attacttgcc atcaccgtta gatatcccag ctatcaaagg gattgaccct aaaactgatg
    43861 aagaagtaga acgtcctgca gacgacagcg ctcctttctc agctttagca tttaaagtta
    43921 tgactgaccc attcgtaggt cgtttaactt tcttccgtgt gtactctggt gttttacaat
    43981 caggttcata cgtacaaaac gcaacaaaag gcaaacgtga acgtgttggt cgtatcctac
    44041 agatgcatgc caactctcgt tcagaaatct ctgaagttta cgctggagat atcgctgctg
    44101 ctgttggttt gaaagacaca actactgggg acacactatg tgatgaaaag agtctagtta
    44161 ttcttgaatc catggaattc ccagaaccag ttatccaagt cgctattgaa cctaaatcaa
    44221 aagctgacca agataaaatg ggtgtggctt tacaaaaact atctgaagaa gatcctactt
    44281 tccgtgctga aacaaacgtg gaaacaggcg aaacaatcat cgctggtatg ggtgaacttc
    44341 acttagatat catcgttgac cgtatgcgtc gtgaattcaa agttgaagct aacgtaggtg
    44401 cgcctcaagt atcttaccgt gaaactttcc gtgctggcac tcaagccgaa ggtaagtttg
    44461 tacgtcagtc tggtggtaaa ggtcaatacg gtcacgtatg gattgaattt acaccaaatg
    44521 aagaaggtgc tggtttcgaa tttgaaaatg cgatcgtcgg tggggttgtt cctcgtgaat
    44581 acattccagc tgttgaaact ggtttgaaag atgctatgga aaatggtgtc ttagctggct
    44641 atccattagt agatatcaaa gctaaacttt acgatggttc ataccatgat gtcgattcaa
    44701 atgaaactgc cttccgtgtt gctgcttcta tggcattacg tgcagcagct aagaaagcta
    44761 atccagtaat ccttgaacca atcatggctg tagaagttgt tatccctgaa gattatttag
    44821 gtgatgttat gggtcacgta actgctcgtc gtggacgtgt tgaaggtatg gaagctcgcg
    44881 ctggcggcca acaagttgtt cgtgcgatgg ttccacttgc tgaaatgttt ggttatgcta
    44941 caactcttcg ttctgctact caaggtcgtg gtacatttac aatgacattt gaccactacg
    45001 aagatgtacc aaaatcagta caagaagaaa tcattaagaa aaacggcgga aaagctgaat
    45061 aatttcttaa aaccggatag tcctgcaaag agattatcca aagagactat atttttaata
    45121 tagtttcgtg taaaataatt atcgatgata tgggcatgtg gtgtaccttg tgtacatcat
    45181 atacctatca gacaaataaa aaatatattg tatttttagg aggacatttt aaaatggcaa
    45241 aagaaaaatt tgaccgttct aaaccacacg ttaacattgg tactatcgga cacgttgacc
    45301 atggtaaaac tacattaaca gctgcaatca caactgtact atctaagaaa aacggcggcc
    45361 aagctatggc ttacgatcaa atcgatggtg ctccagaaga acgcgaacgc ggaatcacaa
    45421 tctcaacagc tcacgttgag tatgaaacag atacacgcca ctacgctcac gttgactgcc
    45481 caggacacgc ggattacgtt aaaaacatga tcactggtgc tgctcaaatg gacggagcta
    45541 tcttggtagt ttctgctgct gacggcccaa tgcctcaaac tcgtgaacac atcctattgt
    45601 ctcgtcaagt tggtgttcct tacatcgttg tattcttgaa caaagtagac atggttgatg
    45661 acgaagaatt actagaatta gttgaaatgg aagttcgtga cctattaaca gaatacgaat
    45721 tccctggtga cgatgttcct gtagttgctg gatcagcttt gaaagctcta gaaggcgacg
    45781 cttcatacga agaaaaaatt cttgaattaa tggctgcagt tgacgaatac atcccaactc
    45841 cagaacgtga caacgacaaa ccattcatga tgccagttga agacgtgttc tcaattactg
    45901 gacgtggtac tgttgctaca ggtcgtgttg aacgtggaca agttcgcgtt ggtgacgaag
    45961 ttgaagttgt tggtattgct gaagaaactt caaaaacaac agttactggt gttgaaatgt
    46021 tccgtaaatt gttagactac gctgaagctg gagacaacat tggtgcttta ctacgtggtg
    46081 ttgcacgtga agacatccaa cgtggacaag ttttagctaa accaggtaca atcacacctc
    46141 atacaaaatt ctctgcagaa gtatacgtgt tgacaaaaga agaaggtgga cgtcatactc
    46201 cattcttcac taactaccgt ccacaattct acttccgtac aactgacgta acaggtgttg
    46261 ttgaattacc agaaggaact gaaatggtta tgcctggcga caacgtaaca atggaagttg
    46321 aattaatcca cccaatcgct atcgaaaacg gtactaaatt ctcaatccgt gaaggcggac
    46381 gtacagttgg tgccggtgtc gttacagaaa tcaaagctta attcacttaa gttttaaagt
    46441 aaagcaacat ttattcggac gtaatcgctc ggaacgaagg tcttaggatc tttgttccga
    46501 gcttttttat ttattgcagg atcaggaaaa aagacaggag tattataaat agaagaaaca
    46561 cgaaataaaa gagtcagtat tttctaaatg aaactttatg aaaacaaaat gttttttaca
    46621 agaaaaagcc ttgctttgtg agtttatata tattataatg acaaaggtgc gtttacttta
    46681 gtgaattgca cagacgtaat cagaatgatt tcgtcggcta agaaacgaga ggttgcgaca
    46741 cgcccggacg ctttgccacg aacgagcgtg acggaaattt tcgtggagct atgtctactt
    46801 ttcaaaatag gcgaaggagg gaacaagatg gcaaaacaaa aaattcgtat ccgtttaaaa
    46861 gcgtatgaac accgtatttt agatcaatca gcggataaaa tcgtggaaac tgcaaaaaga
    46921 actggagcta gcgtgtctgg tccaattcca ttaccaactg aacgcagtct ttacacaatc
    46981 atccgtgcga ctcataaata caaagattca cgcgaacaat tcgaaatgcg tacacacaaa
    47041 cgtcttatcg acattgtgaa cccaacacca aaaactgttg atgctttgat gaagctagac
    47101 ttaccatctg gtgtaaacat cgaaatcaaa ttataaaact aaaacaaaaa taatggaggt
    47161 gtactcatga ccaaaggaat cttagggaaa aaagtgggaa tgacacaaat cttcactgaa
    47221 tctggtgaat taattccagt aactgttgta gaagctactc caaacgtagt actacaagtg
    47281 aaaacaatgg aaacggatgg ctacgaagct attcaagttg gttaccaaga tatgcgtgaa
    47341 gttttatcaa acaaacctgc gaaaggtcat gttgcaaaag caaacacggc tcctaagcgc
    47401 ttcattcgtg aattcaaaaa tgttgagcta ggagaatatg aagtaggaaa agaaattaca
    47461 gtagatgttt tccaagcagg agacattatt gatgtaacag gtactacgaa aggtaaagga
    47521 ttccaagggg ttatcaaacg ccacggacaa agccgcggac caatggctca cggttctcgt
    47581 taccatcgtc gtcctgggtc aatgggtcca gttgcaccta accgtgtatt taaaaacaaa
    47641 cgtcttgctg gccgtatggg tggcaaccgc gtaactatcc aaaacttgga agttgtacgt
    47701 gtcgatgcag aaagaaatgt tatcttgatc aaaggtaatg ttcctggagc taaaaaatct
    47761 ttaattacta tcaaatcagc tgtgaaagct aaataattgt aggaagaaag gaggaactaa
    47821 ggaatgccga atgtagcatt attcaaacaa gatggaagcc aaaacggtga aatcacactt
    47881 aacgaagaaa tcttcggaat tgaacctaat gaaagtgttg tttacgatgc aatcgtaatg
    47941 caacgtgcgt cattaagaca aggaacacac gcggttaaaa accgtagcgc tgttcgcggc
    48001 ggcggacgta aaccatggcg tcaaaaagga acaggtcgtg cacgtcaagg atctatccgc
    48061 tcaccacaat ggcgtggagg tggcgtagtc tttggaccaa caccacgttc ttacagctac
    48121 aaacttccta aaaaagttcg tcgtttagca atgaaatctg tattgtcaga aaaagttgct
    48181 gaaaacaact tggtagcaat cgaaggcttg aacttcgacg caccaaaaac aaaagaattc
    48241 aaacaagttc ttgctaactt atctatcgac tctaaagtat tggttgttct agaaaaaggt
    48301 aacgactttg ctgctttatc agcacgtaac ttaccaaacg tttctgtagt aacttctgat
    48361 aatgtgagtg tgctagacgt tgtatcaaat acaaaagtgt tagcaacaca aactgctctt
    48421 actcaaattg aggaggtgct tgcataatga acttactaga cgtaatcaaa cgcccagtga
    48481 tcactgaaaa atcaatgctt gccatggatg acaagaaata cacttttgaa gtggacactc
    48541 gcgcaaacaa aactttagta aaacaagcgg tagaagctgc ttttgacgtt aaagtcaaaa
    48601 acgtgaacat cgtgaacgta cgtgcgaaat tcaaacgtat gggcaaatat gcaggatata
    48661 caaaaaaacg tcgcaaagcg atcgttacat tgactgaaga ttcaaaagaa atccaattat
    48721 tcgaagctgc tgaataagca ctaatagata actcattaaa ttaggaggga aaaacgtggc
    48781 gattaaaaag tacaaaccta cctcaaatgg tcgtcgtaac atgacaggtt ctgattttgc
    48841 tgaaatcaca acatcaacac ctgaaaaatc attgttacaa ccattgaaaa accatgctgg
    48901 acgtaacaac aacggccgca tcactgtacg tcatcaaggt ggcggacaca aacgccaata
    48961 tcgcgtgatc gacttcaaac gtaacaaaga taacgttgtt gcaactgttc aaacgatcga
    49021 atatgatcca aaccgttcag ctaacatcgc gttagtacac tatgaagatg gagtgaaagc
    49081 ttacatctta gcacctaaag gactagaagt gggtatgaaa cttgtttcag gtccagaagc
    49141 agatatcaaa gtcggaaatg ctcttccatt agaaaacatt ccagttggta ctgttatcca
    49201 caacattgaa atgaaacctg gtaaaggtgg acaattgatc cgttctgctg gtacaagtgc
    49261 tcaagtactt ggtaaagaag gcaaatacgt attgatccgc ttgaactctg gtgaagttcg
    49321 tatgatctta gctgcatgcc gtgctacaat cggttctgta ggtaacgaac aacacgaatt
    49381 aatcaacatc ggtaaagctg gacgctctcg ttggatgcgt aaacgcccaa ctgtacgtgg
    49441 tagcgtaatg aaccctaatg atcacccaca cggtggtggt gaaggtaaag ctcctatcgg
    49501 acgtaaagca ccagtttctc cttggggtca accagctctt ggttacaaaa ctcgtaacaa
    49561 aaaagctaaa tcagacaaac ttattgttcg tcgtcgcaaa aataaataat tactttgacc
    49621 atgtgtcgca cattttgaga ggaggttcac catgggtcgt agtttaaaga aaggaccttt
    49681 tgtcgatgag catttaatga aaaaagttga agctcaagca ggggttgaaa agaaaaaagt
    49741 tatcaaaact tggtcacgcc gttcaacagt tttcccaagt tttgttggat ataccatcgc
    49801 agtttatgat ggacgtaaac atgtaccagt ttacatccaa gaagacatgg taggacacaa
    49861 attaggtgaa tttgcaccta caagaactta tcgtggacat gctgctgacg ataagaaaac
    49921 gaaacgctaa tttgagggga ggataaatcc aaatggcaga acaaatcaca tcagctaaag
    49981 caactgcaaa aacagttcgc acttcacctc gaaaagcccg tttagtaatt gaccttatca
    50041 gaggtaaaag tgtcgcagat gcaatttcaa tcttgaaatt cacaccaaac aaatctgctg
    50101 gaatcattga aaaagtattg atgtcagcag ttgcgaatgc agaaaataac tttgacttag
    50161 atgttgaaaa cttggtagta agcgaagctt ttgttaacga aggacctaca atgaaacgtt
    50221 tccgtcctcg tgcaaaagga tcagcttctc caatcaacaa acgtacaagt catatcacgg
    50281 tagtcgtatc agaaaaataa ggagggataa tcagtgggtc aaaaagtaca tccaattgga
    50341 atgcgtgtag gcatcatccg cgactgggat gctaaatggt atgctgaaaa agagtatgct
    50401 gagttcttac acgaagattt aagaatccgt aaatttattt caactaaact tgctgatgct
    50461 gctgtatcaa cagttgaaat cgaacgcgca acaggtcgcg taaacatttc aattcacaca
    50521 gctaaaccag gtatggttat tggtaaaggc ggttctgaag tcgaaagcct aagaaaagaa
    50581 ttaaacaaat taactggtaa aagaatccat atcaatatcg tggaaatcaa aaaaccagat
    50641 ctagatgcaa aattagtagg tgaaggaatc gcacgtcaat tagaaaaccg tgttgctttc
    50701 cgtcgcgctc aaaaacaagc catccaacgc tcaatgcgtg ctggcgctca agggatcaaa
    50761 actcaagtat ctggtcgtct aaacggagca gatatcgctc gttcagaagg atactcagaa
    50821 ggaactgttc ctcttcatac tttacgtgcg gacattgatt acgcatggga agaagcagat
    50881 acaacatacg gaaaactagg agttaaagtg tggatctatc gtggagaaat tcttccaact
    50941 aaaaaaaaca ctgagaaagg agggaaataa tcatgttagt acctaaacgt gtaaaacacc
    51001 gtcgtgaatt ccgcggaaaa atgcgcgggg aagctaaagg cggaaaagaa gtagcattcg
    51061 gtgaatacgg tttgcaagct gttgattcac attggatcac aaaccgccaa atcgaagctg
    51121 ctcgtatcgc aatgactcgt tacatgaaac gtggtgggaa agtatggatt aaaattttcc
    51181 ctcacaaatc ttatactgcc aaagcaattg gggtacgtat gggttctggt aaaggggcac
    51241 ctgaaggatg ggttgcacca gtaaaacgtg gtaaaatcat gtttgaaatc gcaggcgttc
    51301 ctgaagaagt agctcgtgaa gcgttacgtc tagcttctca caaattacca atgaaaacta
    51361 agatcgtaaa acgtgaggaa atgggtggtg aatcgaatga aggttaaaga aatcagagaa
    51421 ttaaccactg ccgaaatgct tgatcaagaa aaacaattaa aagaagaatt gtttaatctt
    51481 agattccaat tagcaacagg tcaattagaa aacactgcac gtattaaaga agtacgtaaa
    51541 tcgattgcac gcatcaaaac tgtgttgcgt gaacaagcta agtaattatg gaaggaggcc
    51601 attaagcgta tgactgaagg aagaaatcaa cgcaaagttt atcaaggtcg cgtggtttca
    51661 gacaaaatgg ataaaacaat taccgttgct gtcgaaacaa agaaaaacca ccctatctat
    51721 ggcaaacgca tgaactattc taagaaatac aaagtgcatg atgaaaacaa cacagcaaaa
    51781 gttggcgaca tcgtgagaat tatggaaact cgtccattat cagctacaaa acgtttccgt
    51841 ttgttggaag tagtcgaaga agcagttatt atctaataac acgagatttt cctatataga
    51901 ttgaatgcaa aatctgaaag gaggatacac aacgtgatcc aagcagaaag tcgtttaaaa
    51961 gtcgctgata actcaggcgc acgtgaaatt ttgacgatca aagtccttgg tggatctggt
    52021 cgtaaaactg ctaacatcgg tgatgtgatc gttgctacgg ttaaacaagc aacgccaggt
    52081 ggggttgtca aaaaaggtga cgtcgttaaa gccgttatcg ttcgaacaaa atcaggagct
    52141 cgtcgtacag acggttcata tattaaattt gatgaaaatg ctgcagtgat tattcgtgat
    52201 gataaaagcc ctcgtggaac acgtatcttt gggccagttg cacgtgaact acgtgaaaac
    52261 aacttcatga agatcgtttc actagcacca gaagttttat aatcttgaga cacttatcaa
    52321 aggaggtgcg aaacagttat gtttgttaaa aaaggcgata aagttaaagt tatcactgga
    52381 aaagacaaaa acaaagaagg cgttgtacta gcagcgttcc cgaaaaaaga taaagttatt
    52441 gtcgaaggtg taaatgtcgt taaaaaacac caaaaacctt ctcaagctgc tcctcaaggt
    52501 ggaattgttg agatggaagc gccaattcat gtttctaacg tgatggtggt tgactctact
    52561 ggtgtagctg gccgagttgg ctacaaagaa gtagacggca aaaaagtccg tgtttctaaa
    52621 aaaaccggtg aagttttaga taaataaaag tttaaggaag gagggtctta ctgaatgaac
    52681 cgcctaaaag aaaaatatct taaagaagta actccatcat tgatggaaaa atttaactat
    52741 agctctgtta tgcaaacacc taaagttgaa aaaatcgtca tcaacatggg tgttggtgat
    52801 gcggtatcaa acgctaaaaa cttagataaa gcagttgaag aattaacttt gatcactggt
    52861 caaaaaccaa tgattacaaa agctaaaaaa tcaatcgctg gattccgttt acgtgaagga
    52921 atgccaattg gtgcaaaagt tacattgcgc ggagaaagaa tgtacgaatt cttagacaaa
    52981 ttggtttctg tttcactacc acgtgtacgt gacttccatg gtgtaagtaa aaaagccttt
    53041 gacggtcgtg gaaactatac tttaggtatt aaagaacaat tgatcttccc agaagtagat
    53101 tatgatttag tagataaagt acgtggtatg gacatcgtta ttgtaacaac agcaaactct
    53161 gatgaagaat ctcgtgagtt gttgacacaa ttaggcatgc cattccaaaa ataattaaag
    53221 gaggcgaact acgtggctaa aaaatcaatg attgctaaaa acaaacgtcc tgcaaaattt
    53281 tcaacacaag cttatactcg ttgcgaacgt tgcggacgtc cacattcagt ttatcgtaag
    53341 ttccatcttt gccgtatttg cttccgcgaa cttgcctata aaggtcaaat tcccggcgtg
    53401 aagaaagcta gctggtaaaa agtaaattcg cataagaagg aggtaaaaga tcaatggtca
    53461 tgacagatcc aattgcagat tttctaactc gtatccgtaa cgcaaacatg gttaaacatg
    53521 agtctttaga agtacctgca tcaaaaatta aacgtgacat cgctgaaatc ttgaaacgtg
    53581 aaggtttcgt tcgtgatgtt gaatacatcg aagatgacaa acaaggcgtg atccgtgttt
    53641 tcctaaaatt tggtaaaaac ggcgaacgtg ttatcacaaa cttaaaacgt atctctaaac
    53701 caggtttgcg tgcttatgtc aaagctgacg aagtaccaaa agtattgaac ggtttaggta
    53761 tcgcaatcat ttcgacttct gaaggtgttg tcactgataa agaagctcgt gaaaaaaata
    53821 tcggcggcga agttatcgct tacgtatggt aatcaagtag aatctacaag gaggtgtctt
    53881 taagtgagcc gtatcggaaa taaagtcgtt gttatccctg aaggagtaac agttactcaa
    53941 gacggaaaca acgttacagt taaaggacca aaaggtgaat tgactcgtac tttttcagca
    54001 gatatcaaaa tgaatatcga aggaaacgaa gtaactttca ctcgtccaaa cgacagtaaa
    54061 gaaatgaaaa caattcatgg aacaactcgt gcgaacttca acaacatggt tgtaggtgtc
    54121 agcgaaggtt tccaaaaagc gttagaacta atcggggttg ggtaccgtgc acaattacaa
    54181 ggatcaaaac ttgtattgaa cgttggttac tcacatccag tagaaatcac accaccagct
    54241 ggagtaactg ttgaagtacc agctaacaca caagtaattg ttaaaggtgc gaacaaagaa
    54301 gaagttggcg aattagctgc aaacattcgt ggaactcgtc ctccagaacc ttataaaggt
    54361 aaaggtattc gttatgttgg cgaatatgta cgccgtaaag aaggtaaaac tggtaaataa
    54421 tcgtttagaa aaattgcata gaagccagca gcaggaaaaa ggcatttggc tgaagtttac
    54481 cttcaaaaaa atacattttt acttgtgccg cagcttctat ttttcgaaac gtatttactg
    54541 cttgaaatag tagtacaata aaaataaaga ggtgacaatt gtgattacaa aaccagataa
    54601 gaacaaaaca cgtcaaaaga gacatcgtcg tgtacgtaac aatatctctg gtactgctga
    54661 gcgcccacgc ttgaacgttt ttcgttctaa caaaaacatc tacgctcaaa ttattgatga
    54721 cgtagcgggt gtaacgctag caagtgcctc taccttggat aaagaaattt caggtggaac
    54781 aaaaacagaa gcagcacaag ctgtcggtaa actagttgct gaacgtgcag ctgaaaaagg
    54841 cattaaagta gtagtctttg accgtggtgg atacctttac catggccgtg tgcaagcttt
    54901 agctgaagct gctcgcgaaa atggactaga attttagaag aaggaggaat accattcatg
    54961 gtttatatcg atccaaaaca tttggaatta gaagaccgcg ttgttgcaat taaccgtgta
    55021 acaaaagttg ttaaaggtgg acgtcgtcta cgttttgctg ctttagttgt tgtcggtgac
    55081 aaaaacggac acgtagggtt tggtacaggt aaagctcaag aagttcctga agcaatccgt
    55141 aaagcaattg aagatgcgaa gaaaaactta gttgaagtac ctatggttgg ttcaacaatc
    55201 ccacacgaag taatcggtgc ttttagtggt ggtagaatcc taatgaaacc tgctgtagaa
    55261 ggttctgggg ttgctgctgg tggaccagtt cgtgccgtat tagaattagc tggggtagca
    55321 gatatcactt ctaaatcttt aggctcaaat acaccaatca acgttgttcg cgcaacagtt
    55381 gaaggtttga aacaattgaa acgtgcagaa gaagttgcag aacttcgcgg caaatcagtt
    55441 gaagaattaa tcggataagg aggacaacaa taatggctga attaaaagtt actttaaaac
    55501 gcagtattat cggacgtcct caaaaccaac gcgatacagt taaagcgtta ggtcttacaa
    55561 agattaacag ttcagtggtt aagccttcta atgaagcaat taaaggcatg atcaatactg
    55621 tttctcacct agtggacgtt gaagaaattt aatttagaaa aatcataact agaacattaa
    55681 ggaggtgcca aacctatgaa acttcatgaa ttaaaacctg ctgaaggttc acgccaagta
    55741 cgtaaccgtg ttggtcgtgg tacttcatct ggtaacggaa aaactgccgg acgcggacaa
    55801 aaaggacaaa aagctcgttc aggcggcggt gtgcgtttag gatttgaagg gggacaaaca
    55861 ccattgttcc gtcgtttacc aaaacgagga ttcacaaacg tgaaccgtaa agactatgca
    55921 gtcattaatc ttgatgtatt gaaccgcttt gaagacggag ctgaagtaac tcctgttgct
    55981 ttagttgaag ctggaatcgt gaaaaacgaa aaagctggaa tcaaagtatt agctaatggg
    56041 gaactaaaca agaaattaac tgtgaaagca gcaaaattct ctaaagcagc tcaagaagca
    56101 attgaagcgg ctggtggatc aatcgaggtg atctaatgtt tagactatta aaagatgctt
    56161 ttaaagtaaa agacattaga tcgaaaatcc tatttactgt atttgtgttg tttgtatttc
    56221 gcctaggtgc acatattact gtaccaggcg taaatgctgc ttcattgcaa aacctttctt
    56281 ctttgccatt tttcaacatg ttgaacttgg taagtggtag tgcgatgcaa aacttttcca
    56341 ttttctcaat gggggtttca ccatacatta ctgcttctat cgtcattcag cttcttcaaa
    56401 tggacatcgt gcctcgattt gttgaatggt cgaaacaagg ggaagttggt cgtaaaaaac
    56461 tgaatcaagc gactcgatat ctgacattag tattggcatt tatccaatct gtcggtatca
    56521 ctgctggatt caatcaatac gctcaattag gtttcgtgaa taatcctaac gtagcaactt
    56581 acgtaacgat cggtttgatt ctaacagccg gtacgatgtt tgttacttgg ctaggagaac
    56641 agatcacaga aaaaggtatc ggaaatggcg tatcaatgat catttttgca ggtatcattt
    56701 ctagattgcc tgaaagtatc agagaaattt atgaagatta ctttataaat attgatcctt
    56761 cagacatatg gaaatctgct atttttgtgg ccattttact agtagcggtc ttgttgatcg
    56821 tgacattcgt aacatttttc caacaagctg aacgcaagat cccgatccaa tatacgaaac
    56881 gcgtagctgg cgcgcctaca agcagctatc tgccgttgaa agtaaatgca gctggagtta
    56941 ttccagttat ctttgctagt tcgtttattg ctacgccaaa tgctgtttta caagcattga
    57001 gcggtagatt cggttctgaa aactggtaca aagtgatgat ggagatcttt aactataata
    57061 cagcacctgg tgctacgatt tatacgattc tgatcgttgc cttcactttc ttctacgcct
    57121 ttgttcaagt aaaccctgag aaattagcgg aaaacttgca aaagcaagga agttatatac
    57181 caagcgtacg ccctggtaag gggactgaag agtatgtctc aagattactg atgcgtttaa
    57241 gtacagttgg tgcagtcttc cttggtttag tggcattgct tccaatcatt gctcaaatgg
    57301 tttgggatct tccacaatca atcggattag gcggtacaag cttactgatc gttatcggtg
    57361 tagctttaga aactgcgaaa cagttagaag gcttaatgct gaaacgtaaa tatgttggat
    57421 tcattgagta agaaagcgtg ttgaggtttc ccctcaacac acgttctcac acatttatta
    57481 ggagggaaaa caatgaacct cattttaatg gggctgccag gcgcaggtaa aggaacacaa
    57541 gctgaaaaaa tcattgatgc ttatggtatt ccgcacatct caacaggaga tatgttccgc
    57601 gcagcgatgc aaaacgaaac agctcttggc ctggaagcaa agtcttatat ggataaaggt
    57661 gcattagttc ctgatgaagt aacaaatgga atcgtgaaag agcgactagc tgaaccagat
    57721 acagagaaag gcttcttatt agatggcttt ccacgtacgc tagatcaagc aaaagctctg
    57781 gacgcaatgc taaaagattt gaataaaaaa attgatgctg tcatcgatat ccatgttggt
    57841 gaagaaatct tggtcgaacg tctagccggc cgatttatct gcagcaattg tggagcaact
    57901 taccataaaa ttttcaaccc tacaaaagtt gaagacacat gtgatcgttg cggtgggcat
    57961 gaattctatc aaagagaaga tgataaacct gagacggtta aaaatcgtct ggctatcaac
    58021 atcaaaaata gtgaaccaat cttggcttat tataaagaac aaggtttgct aaacactatt
    58081 gatggtgctc gtgaaatcga tgctgtattt gcagatgtac aaaaaatcat tgaaaaatag
    58141 cacgattgcc cgtagccgac ttgcattatg cagttactct tgtcaagttc aataaattat
    58201 gatagaatat gtcagtccga cttctagaga atcctctaga tgatccattt tgagaatttt
    58261 gaggtgggaa ctgcagtttc cgccatttta tcgtgtgaaa atgcgaaaca agtacaagga
    58321 ggtactgtgc gtggcaaaag aagatatgat tgaagtcgaa ggtacagtcg tcgaaacttt
    58381 gccgaatgca atgtttaaag ttgaactaga aaacggacac caagttcttg ctactgtttc
    58441 aggtaaaatt cgtatgcatt acatccgtat cttacctggt gacaaagtaa cggttgaatt
    58501 gtctccatac gatcttaacc gtggccgtat tacttatcgc tttaaataat tgtactccgt
    58561 aaaactacag gaggtattat catgaaagta agaccatcag taaaaccaat gtgcgaacat
    58621 tgtaaagtaa ttcgtcgtaa aggacgcgtt atggtgattt gcccagcaaa tccaaaacat
    58681 aaacaacgtc aaggataatt ggaggtgcta taaatatggc tcgtattgca ggagtagata
    58741 ttcctcgtga taaacgtgta gtagtttcac ttacttatat ctatggtatt ggtaacacta
    58801 ctgctaaaaa gatcttagct gatgctggtg tatctgaaga tgttcgcgtt cgtgaattaa
    58861 cgaatgaaca aacagatgct atccgtgcag aagtagataa attaaaagtt gaaggggatc
    58921 ttcgtcgtga agtgaacttg aacatcaaac gtttgatgga aatcggttct taccgtggaa
    58981 tccgtcatcg ccgtggattg cctgttcgtg gacaaaacac gaaaaacaat gcacgtactc
    59041 gtaaaggccc agctcgcgct atcgctggca agaaaaaata attcttaagt gaaggaggtt
    59101 aaaacttcat ggcagcaaaa aaagtgaatc gtaaacgccg tgtgaagaag aatatagaag
    59161 caggtattgc acatatccat tctacattca ataacactat catcatgatc actgatactc
    59221 acggaaatgc attagcgtgg tcatcagctg gttcattagg tttcaaagga agcaaaaaat
    59281 caactccttt tgccgctcaa atggcagcag aaactgctac aaaagcagca atggaacacg
    59341 gattaaagac tgttgaagta acagttaaag gacctggttc tggacgtgaa gcagcaattc
    59401 gttcattaca agctacaggt ttagaagtga ctgcaattcg tgacgtgact ccagttcctc
    59461 ataatggtgc ccgccctcca aaacgccgtc gtgtttaatg agtgccgctc attttgagtt
    59521 ttatcagtaa cgtcattgaa caagacactt ttcgttttga aaggggtaaa gataagaatg
    59581 attgagttcg aaaaaccaag aatcgcaaaa attgatgaag aaaaagatta tggcaagttc
    59641 atcgttgaac ctcttgaaag agggtatggg actactttag ggaattccct acgtcgtatt
    59701 ttattatctt ctttgccagg tgctgccatc actaatattc aaatcgatgg tgtactgcat
    59761 gaattctcta ccatcaaagg tgttagagaa gacgtcactc aaatcatctt gaacattaaa
    59821 ggcttagcgc tgaaaatgta tggccaagaa gaaaaaaccc ttgaaatcga tattaccgga
    59881 cctgctacag taactgctgg cgatattatc gttgatagtg atgtagaaat tcttaacaaa
    59941 gatatgtaca tctgtagtgt ctctgaagga gctacgttcc atgctcgctt aacagtgaaa
    60001 cctggacgtg gttatgttca agcagatgaa aataaaaaag aagatatgcc aattggcgtt
    60061 cttccagtcg attcaatcta cacacctgtt cgtcgtgtga actatcaagt tgagaacaca
    60121 cgtgttggac accgtgaaga ttttgataaa ttgacgatgg agatttggac agatggttca
    60181 attgaaccgt tggaagcaat gagtttgtct gcgaaaatca tgactgaaca tttggatatt
    60241 tttgtgaact taactgatga agcaaaaaat gctgaaatca tggtcgaaaa agaagaaaca
    60301 caaaaagaaa aaatgttgga aatgacgatc gaagagttag acttgtctgt tcgctcttac
    60361 aattgtttga aacgtgcagg aatcaatact gtacaagaac ttacaaataa atctgagcct
    60421 gaaatgatca aagtccgtaa cttaggacgt aaatcattag aagaagttaa agcaaaattg
    60481 cacgacttag gtttaggttt acgtaaagaa gattaaaacc ttttacgaag gagggaaacc
    60541 aacgtgagtt atcgtaaact aggacgcaca tctagccaac gtaaagcgat gttgcgtgat
    60601 ttaacaactg atttattaat caacgaacgt attgtgacta ctgaagctcg tgcgaaagaa
    60661 gttcgctcaa ctgcagaaaa aatgatcact ttaggtaaac gcggagattt gcatgcacgt
    60721 cgtcaagctg cggctttcgt acgtaatgaa gttgccagcg tgcgtgaaga aaatgaggac
    60781 atcgtcattg aatcagcttt acaaaaactg ttcaacgata ttgcacctcg ttacgcagaa
    60841 cgtcaaggcg gatacacacg cattttgaag acagaaccaa gacgcggtga tggtgcacca
    60901 atggttgtct tagaacttgt ctaaaatcac actaataaca tcactttata gatgattctt
    60961 ttagagtgtt atgatgttgg aagatatttt tccgagtcta gctcagcgct gctcctcaag
    61021 cgaatatcgc ttggggagta agcactcatc ataaagtgtt tttttatacc ttcaattacc
    61081 tgtcatgaca gatatgtttg aaggcataaa aaaacatttt aagcgagaaa taattgaaaa
    61141 ttttgtcttt ttttgatagt gttagtagga actttaggat gaagaaggag taatcatgga
    61201 gccaattatt gaattagaaa agataaatta taaataccag ccagatgatc ttcgccccgc
    61261 attgaaagat gtctccttta caattgataa aggtgagtgg atcgctatta ttggacacaa
    61321 tggttcagga aaatcgacac ttgccaaaac gatcaatgga ttgcttctgc cagaatcagg
    61381 gatcgtgaaa gtgggaaatc aaatattgga tgaagaaaat atttggacga tccgacagat
    61441 ggttggaatg atttttcaaa atccagacaa tcagtttgtt ggttcgacag tagaagatga
    61501 cgttgctttt ggtcttgaaa atcaagggat tcccagagaa gaaatgctag tgcgtgtgaa
    61561 agatgcactt gagaaagtta gaatggctga gtttgcttca cgcgagcctg ctcgtttatc
    61621 aggagggcaa aaacaacgtg ttgccattgc cggcgtggtt gccttacgac ctgatatcat
    61681 tattttagat gaagcaacca gtatgctaga tccagaaggt cgtgaagaag tgatctctac
    61741 gatcaagaag atcaaagaag aaagtcaatt gaccgttata tctatcactc atgatattga
    61801 tgaagcagct aatgccaatc ggatactagt gatgagacaa ggcgaacttg tccgtgaagg
    61861 tacaccaaaa gaaatttttt ctgctggtcc tgaactgatc gacttaggtc tagatctacc
    61921 atttcctgaa aaactgaaga gcgcactgaa agaacgtggg gttgatgtgc ccagtgaata
    61981 tatgacagaa gaaaggatgg tggattggtt atggacatcc gttttgaaca agtagacttc
    62041 acttatcagc cgaatacacc atttgaacaa agagccttat ttgacatcaa tatgacgatc
    62101 aaagaaaaca gttatactgc attagtaggg cataccggaa gcgggaaatc aaccttactt
    62161 cagcatttga atgcgcttgt taagccaaca agcggtacag tccatattgg agaacgagat
    62221 attcagccgg atacggataa taagaattta aagcccatcc gaaaaaaagt tggcatcgtc
    62281 tttcagtttc cagaagcaca gctattcgaa gaaacggtag caaaagacat tgctttcggt
    62341 cctaaaaact ttggtgtcag cgaagaagaa gcactagtcc tagcaaaaga aacattagaa
    62401 caagttgggc tggatgaaag ctatttggaa cgttcgccat ttgaactttc aggagggcaa
    62461 atgcgtagag tagcaatcgc tggtgtgctt gccatgaggc cggaagtact tgtattggat
    62521 gaaccaacgg caggacttga tccccagggc agaaaagaaa tgatggagat gttttggcgt
    62581 cttcataagg aacagcagat cactattgta ttagttacgc atttaatgga tgatgtagcc
    62641 aattttgcag attatgtcta tgtgttggaa aagggacgaa tcgttaatag cggggagccg
    62701 caagaggtct ttcaaaacat tgaatggcta aaagaaaaac aactaggcgt acctactgcc
    62761 accgagtttg cagaagaatt aatggctaaa gggatgaact ttgctacctt gcctttgaca
    62821 gctgaagaat tagcagacgc tatcgtccat tatgctggag gaaatgccca tgatgaataa
    62881 attgatattt ggtcgttata tgcctggaga ttccttgatc catcgattgg atccccgagc
    62941 taaattatta gccagttttt attttatcgg aatcattttt ttagcaaata attgggaaac
    63001 gtaccttttg ttggcatttt tcacattact ttctgtcttc ttgtcaaaag tagatttgaa
    63061 attttttatc cgaggcattc gcccattgat ctggctaatc ctcttcactg tagctttgca
    63121 ggtcttgttt acaagtggcg gagaaactta ttggagttgg ggtgttttcc atatcactga
    63181 atttggtgtg atgaacggcc tctttatttt ctgtcgattc gtgttgatca tttttatgtc
    63241 gactctactc acgcttacta caccgccttt ggaattatcg gatgcgattg agtacttgct
    63301 tcgtccattg aaagccgtac ggttcccagt ccacgaaatt tcattgatgc tgtcgattgc
    63361 tcttcgtttt gtgcctacat tgatggacga aacagaaaag atcatgaacg ctcagcgcgc
    63421 tcgcggtgtt gattttggtg aaggaaacct agttcagaaa atgaaagcag tcattccttt
    63481 gctgattcct ttatttgtca gcagcttcaa ccgtgcggaa gatcttgcga ctgctatgga
    63541 agcaagaggc tacaaaggtg gggaaggaag aacgaaatat cgtgtattgc attggcataa
    63601 tagagacacg atggttatca ttgcttttgt attattaact gtgctactga tctttttaag
    63661 aggataaacg ttcagtgggc cgctatctat tcttttcttg ctgatctgtt tgcaagaaag
    63721 gatgcaagca gtgtatgaca agaaatatta gggtctgtgg cagactaagg tcacaggcct
    63781 ctttctttta tctgaaaaga aagaacgaaa aaattttata gaaaaatcat tattccagat
    63841 aaccttttag gaacaaagga gagaaaaaca tggtacgtta caaagcaatc attgcgtacg
    63901 acggaacaaa ttttaatgga ttccaaaaac agccaaatgg gcgtactgtt caagaagaag
    63961 tagaaaaaac attacaaaaa atggcaaatg gaaaagaaat cacggtcttt ggttcgggaa
    64021 ggacagatgc cggagtacat gcgatcgggc aagtaatcca ttttgattat ccagaggagc
    64081 gtccgttaga acgaatgcgt ttcgcgctag atacccagtc accagaggat attgctgtaa
    64141 gacaagtaga aattgtttca gaagacttcc acgcaagata tttggtcaaa gaaaagacgt
    64201 atcaatttcg agtggatata gggaaaccac gcagtccttt tcgacgccat tatgcaagtt
    64261 attttcctta cccacttgat ttggaaaaaa ttcagcgggc gcttcctgat ttattaggga
    64321 ctcacgattt cacctctttt tgtgcatcag gaagctcgat agaggataaa atacgtacga
    64381 tctacgaggc taagatggag gtaaatgaaa caaaagacga attgttgttt acatttaggg
    64441 gaaatggctt cttgtataag atgatacgga tcttagtagg aacgcttttg aaaattggca
    64501 atggccgatt gcctgaagat agtatcccag atattattgc gaaaaaagat cgtaatgcag
    64561 caggacctac agcacaccct gaaggacttt atttgtatga agtgaaatat gaataagagg
    64621 ttatgataga agtggtcagc ttcaagaaat aagaaggtat ttacgaaaat tacttctcaa
    64681 aattttgcaa aatttcggct tattttcgaa gaagctactt ctggaacacc gtttaagaaa
    64741 gaggtcgtga gcctgtcatt taactctgag tcacaaagag caatttcaaa aaacagcttc
    64801 tcatctttga tgaatatcgt gacttgtgga cgaggcgttg ataagtgaat catctttttg
    64861 ttacctattt tttctgtaac caaaaaacga tggaacgttg ttttgattgc ccttggtgcg
    64921 gacagtctga acaggttcca tcgtttcttt attttctacg tcttcttctg tgtgtgtttc
    64981 gtggttgttc ttctattgct ggatcaatcg tgccataaaa ggggcgattg cggtcaaggt
    65041 accagacata aagaataaac aatgcaatac ataggacaaa aaggattgca cctatcgtaa
    65101 atccttcagg caagtaactg aaagtgacag tatgtttacc tgctggaact tttatcatca
    65161 agaatgcgtc tttgaaattg ctgaccttga ctggtttccc atcaatttta gctttccatc
    65221 ctttgtcata aggaatagtt gtcaccaaca tggaatcttc agcggtattt acagtcccag
    65281 aagcttttct gccagtggct tccatttcga cacctttttc tttgatctga ttcattgcgg
    65341 cttgataggc tacaacatct agaccaacga ctttaggctc catgaaactt atttctttcg
    65401 tcccataaaa acttgcagta aatgtcactg ttgtctcttg aggataatag ccaagatcat
    65461 agtattgacc agaaatgttg atttgcgttt ttcgcgatgt accattgaca gtgatcgtcg
    65521 cactagaact ttctaattgc gcaaaattgg ttgggaacaa acttaagtaa gcttgtgtat
    65581 ttgcaggcac atggacttcc caagtgagct cttttggctg gttatctgct gtttctgagt
    65641 aagtgacgcc agtcgttgtc tttgtgatcg tcgtattcaa ctgattcttc aaagtaggtg
    65701 tatagaatgt aaaatactgt tcatttagac cagacaatgt attgatcaag tttgtctgac
    65761 tggtcaaatt atctgtcact ggctgctcaa catcattaac ggacaacggt gcagtaaatg
    65821 ctagtggcaa tgcattgaca ttctgataca gtgtatagtc gccgctttga ccagcttggc
    65881 ggaagccata tttactgaac gtgctttcgt ttttagaaat gttatatttg attcctgtaa
    65941 acccatccat cagcaaagta ttattgttat aacggatatt caaatttgtc cctcttgaac
    66001 ggaaacctaa tttatccagg taagaggaag aattacgatt tcttatagaa gaaaacaaac
    66061 tgataccact gtaaccatag ttgatcgaat cgtttgcaga tactggatcc agattttcca
    66121 agcggtaaaa cgagtcggaa tctttttttg tttcatttac aagagctttc aagtcaggat
    66181 aaggatcagt atataaactg cgggaagcat aattccaatc atcaagaatg ccatggatca
    66241 tactgttcgt attgaccagt gcttcagcag acatgatcaa taataacaat atactcagat
    66301 gattgaatga aagtttttta tgctgataaa atcccacagc aaaagcataa atccctaaga
    66361 aaaccactgt caatacaaag gaagtcatcg tcacataggt gtagcttcct tcaggtttga
    66421 tgccccatgc caccgcaaag agagcagcta cgaccaaaat gattcctaac aacagcccac
    66481 catcgtcttg ttctatcttt tcccatcctt ttcctgcaag caggagcact aagaaagaga
    66541 acagataggc ataacggaat aaaaacatat tcggtgcatg cataccatgc cagaacaaat
    66601 tcaatggaac aagataaaaa cttgcgataa gtatggcgaa aagactgccg tacaaaaatt
    66661 tatttttcaa cggtactttc cgactggcaa aataaaacag acagaggact aatggtagca
    66721 gacctgcgta aacgaacgga atcgatccgt attttgtcgt gtcataaaca ccaatcatat
    66781 ttttcatgat tagatccaga taagaagtcg cttctgtttt caacgtagtg atctgactca
    66841 gtgcctcgcc atttgaccga agatccagca ctgctggcaa tacaatgatc attgaagcgc
    66901 cgcctgccag taaagaagtg ataccataag gaacaatcgt ttttttatat tctgaccagt
    66961 ttgtcaatag acgggcgata aaatataaga aagaaaatac gccaatcata aaacccatat
    67021 aaaaactgga taaaaacaag agtaaataac tgataaataa aagtttcggc cgtttaagat
    67081 ccataacacg atgaatcccc aaaatcacta atggaagata aacaaatgca tccagccaca
    67141 tgatgatctc cgaatgtgca gtgataaatg agatcaatgc gtaactgacg ctaagtgcaa
    67201 catgccccca gcgaggaatc ttgaacgtac gtgcaagaaa ccaaaaagcc aatccggcac
    67261 agccgacctt aagcaaagtc agaacataca gtgcatctgg catcatctgg tttggaaaaa
    67321 agaacaccag tggcgtgaag atcccgccta gatagtaaga aattaaagat aaataattca
    67381 atcctagaga agcattccat gtataaaaaa tactttgctt tccaagaaga acatttcgga
    67441 agctggcatg aaaattagaa aactgtgaaa aggcatcact tgccaaaata cttctgctgc
    67501 ttccaggata gattccgata cttagataaa caagtgctaa aatcaaaaac ggaatcagga
    67561 aactagctgc tgcgtagcgt ccgttttctc taaagaatga ctttaaattt tttttcatat
    67621 ttattttcca ccaaatttct atgaagttgt a
//
LOCUS       DARBEG010000004        62950 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000004 DARBEG010000000
VERSION     DARBEG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.004"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            274..594
                     /locus_tag="SCK20_000269"
     CDS             274..594
                     /locus_tag="SCK20_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1144367.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            748..1653
                     /locus_tag="SCK20_000270"
     CDS             748..1653
                     /locus_tag="SCK20_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1144368.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1650..2423
                     /locus_tag="SCK20_000271"
     CDS             1650..2423
                     /locus_tag="SCK20_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1144369.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2604..4028)
                     /locus_tag="SCK20_000272"
     CDS             complement(2604..4028)
                     /locus_tag="SCK20_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1144370.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4239..4685
                     /gene="nrdI"
                     /locus_tag="SCK20_000273"
     CDS             4239..4685
                     /gene="nrdI"
                     /locus_tag="SCK20_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1144371.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4750..6732)
                     /locus_tag="SCK20_000274"
     CDS             complement(4750..6732)
                     /locus_tag="SCK20_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1144372.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6746..7642)
                     /gene="murQ"
                     /locus_tag="SCK20_000275"
     CDS             complement(6746..7642)
                     /gene="murQ"
                     /locus_tag="SCK20_000275"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1144373.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7645..8706)
                     /locus_tag="SCK20_000276"
     CDS             complement(7645..8706)
                     /locus_tag="SCK20_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1144374.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8917..9483)
                     /locus_tag="SCK20_000277"
     CDS             complement(8917..9483)
                     /locus_tag="SCK20_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1144375.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9511..11013)
                     /gene="eat(A)"
                     /locus_tag="SCK20_000278"
     CDS             complement(9511..11013)
                     /gene="eat(A)"
                     /locus_tag="SCK20_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1144376.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11271..12392)
                     /locus_tag="SCK20_000279"
     CDS             complement(11271..12392)
                     /locus_tag="SCK20_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1144377.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12518..13459
                     /locus_tag="SCK20_000280"
     CDS             12518..13459
                     /locus_tag="SCK20_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1144378.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13609..14505)
                     /locus_tag="SCK20_000281"
     CDS             complement(13609..14505)
                     /locus_tag="SCK20_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1144379.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14774..16114
                     /locus_tag="SCK20_000282"
     CDS             14774..16114
                     /locus_tag="SCK20_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1144380.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16332..16775
                     /locus_tag="SCK20_000283"
     CDS             16332..16775
                     /locus_tag="SCK20_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1144381.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17279..18697)
                     /gene="nhaC"
                     /locus_tag="SCK20_000284"
     CDS             complement(17279..18697)
                     /gene="nhaC"
                     /locus_tag="SCK20_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1144382.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19100..20323)
                     /locus_tag="SCK20_000285"
     CDS             complement(19100..20323)
                     /locus_tag="SCK20_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG1144383.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20354..21193)
                     /locus_tag="SCK20_000286"
     CDS             complement(20354..21193)
                     /locus_tag="SCK20_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1144384.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21210..21890)
                     /locus_tag="SCK20_000287"
     CDS             complement(21210..21890)
                     /locus_tag="SCK20_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144385.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21892..22965)
                     /locus_tag="SCK20_000288"
     CDS             complement(21892..22965)
                     /locus_tag="SCK20_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1144386.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23021..23259)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23302..23535)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23612..24283)
                     /locus_tag="SCK20_000289"
     CDS             complement(23612..24283)
                     /locus_tag="SCK20_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1144387.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24370..27048)
                     /gene="mgtA"
                     /locus_tag="SCK20_000290"
     CDS             complement(24370..27048)
                     /gene="mgtA"
                     /locus_tag="SCK20_000290"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG1144388.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27707..28144
                     /gene="ndk"
                     /locus_tag="SCK20_000291"
     CDS             27707..28144
                     /gene="ndk"
                     /locus_tag="SCK20_000291"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG1144389.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28166..29032)
                     /locus_tag="SCK20_000292"
     CDS             complement(28166..29032)
                     /locus_tag="SCK20_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1144390.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29192..31285)
                     /locus_tag="SCK20_000293"
     CDS             complement(29192..31285)
                     /locus_tag="SCK20_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1144391.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32034..32657)
                     /locus_tag="SCK20_000294"
     CDS             complement(32034..32657)
                     /locus_tag="SCK20_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144392.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32816..34213)
                     /locus_tag="SCK20_000295"
     CDS             complement(32816..34213)
                     /locus_tag="SCK20_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1144393.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34245..35216)
                     /locus_tag="SCK20_000296"
     CDS             complement(34245..35216)
                     /locus_tag="SCK20_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1144394.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35590..36015
                     /locus_tag="SCK20_000297"
     CDS             35590..36015
                     /locus_tag="SCK20_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144395.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36503..37204)
                     /locus_tag="SCK20_000298"
     CDS             complement(36503..37204)
                     /locus_tag="SCK20_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1144396.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37220..37660)
                     /locus_tag="SCK20_000299"
     CDS             complement(37220..37660)
                     /locus_tag="SCK20_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1144397.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37660..38235)
                     /locus_tag="SCK20_000300"
     CDS             complement(37660..38235)
                     /locus_tag="SCK20_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG1144398.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39083..39562)
                     /locus_tag="SCK20_000301"
                     /pseudo
     CDS             complement(39083..39562)
                     /locus_tag="SCK20_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39721..40422)
                     /locus_tag="SCK20_000302"
     CDS             complement(39721..40422)
                     /locus_tag="SCK20_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1144399.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40530..41939)
                     /locus_tag="SCK20_000303"
     CDS             complement(40530..41939)
                     /locus_tag="SCK20_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG1144400.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(41987..43852)
                     /locus_tag="SCK20_000304"
     CDS             complement(41987..43852)
                     /locus_tag="SCK20_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1144401.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43973..44824)
                     /locus_tag="SCK20_000305"
     CDS             complement(43973..44824)
                     /locus_tag="SCK20_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1144402.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45134..45847)
                     /locus_tag="SCK20_000306"
     CDS             complement(45134..45847)
                     /locus_tag="SCK20_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG1144403.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45915..46241)
                     /locus_tag="SCK20_000307"
     CDS             complement(45915..46241)
                     /locus_tag="SCK20_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1144404.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46364..46591)
                     /locus_tag="SCK20_000308"
     CDS             complement(46364..46591)
                     /locus_tag="SCK20_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1144405.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46913..48166)
                     /locus_tag="SCK20_000309"
     CDS             complement(46913..48166)
                     /locus_tag="SCK20_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1144406.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48227..48967
                     /locus_tag="SCK20_000310"
     CDS             48227..48967
                     /locus_tag="SCK20_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1144407.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49053..49811)
                     /locus_tag="SCK20_000311"
     CDS             complement(49053..49811)
                     /locus_tag="SCK20_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1144408.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49808..50836)
                     /locus_tag="SCK20_000312"
                     /pseudo
     CDS             complement(49808..50836)
                     /locus_tag="SCK20_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50856..54023)
                     /locus_tag="SCK20_000313"
     CDS             complement(50856..54023)
                     /locus_tag="SCK20_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144409.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54247..55131)
                     /locus_tag="SCK20_000314"
     CDS             complement(54247..55131)
                     /locus_tag="SCK20_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1144410.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55226..55663)
                     /locus_tag="SCK20_000315"
     CDS             complement(55226..55663)
                     /locus_tag="SCK20_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144411.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(55880..56791)
                     /locus_tag="SCK20_000316"
     CDS             complement(55880..56791)
                     /locus_tag="SCK20_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1144412.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(57248..57676)
                     /locus_tag="SCK20_000317"
     CDS             complement(57248..57676)
                     /locus_tag="SCK20_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144413.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(57704..58342)
                     /locus_tag="SCK20_000318"
     CDS             complement(57704..58342)
                     /locus_tag="SCK20_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144414.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(58346..60328)
                     /locus_tag="SCK20_000319"
     CDS             complement(58346..60328)
                     /locus_tag="SCK20_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG1144415.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            60508..61173
                     /locus_tag="SCK20_000320"
     CDS             60508..61173
                     /locus_tag="SCK20_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144416.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT        18348 a        13052 c        10681 g        20869 t
ORIGIN      
        1 tgtttttttt aaatgattaa taaataacaa cttctttcta tgaaattgca aagcattcat
       61 cttcttttcc gctaacgata tatccgtcag tgcttggtac actttactaa aaagtatgag
      121 attgccgata tctggatagg tgtgctatac tacagaaaaa gatatacaat ctgccaccgg
      181 gtaggataaa gaggatgatc attccattag gtctttgaag ggatgatttt cctctttttg
      241 tgtatttttt tatagttaag gaggaatgta agtatggcat ggatggaatt aatcatagca
      301 ggaattttgg aagtattctg gtcaacgatg atgaaatgga gtgatggttt ttctaaaatc
      361 aattattctt tttacacagt gattggcatg attgctagtt tttacttttt gtcaaaggca
      421 atcaaaagtt tgccgatgag tctctcttat cctatctgga cagggattgg cgcagttggt
      481 tcggtcataa ttggtgtggt cttgtttcat gataagctga atctttccac ttggttcttt
      541 gtggggatgc tactcatcag tatcattggc atcaagatta ctagcggcca ctagacactt
      601 ggaataagta ctgatcagaa aaaataagcc tttttattta atattcaatt tttttatgaa
      661 aaaataagaa ataagtccaa tgacctagcg acttctccac tcatcaggtg tatgcttgta
      721 ctatctatag aaaaggagtg aatatttttg aaaagtcttt cggtccaaga attatccaag
      781 aactttggtc acaaacaagc cctcagcgat gtcagtttta gcttatcccc tggcaaaatc
      841 gttggattag taggtcctaa tggcgctgga aaaacaacga tcatgaaagc tattttagga
      901 ctgatccatt actcagatgg aacaatctgt attggtgaaa atgagatttc tccctctagt
      961 cataaagggt tggaacaagt aggagcattg atcgaatacc cgggtatcta tccattctta
     1021 acaggctatg atcatctgaa gttattctct gaaaccaacg atgtatctgc tatcgacact
     1081 atcgtgaatc agctcaagat ggaaaagtac attcacaaaa aagcgaaatc ctactcgttg
     1141 ggaatgaaac aaaaattggg aattgctctt gccttactga accatccgtc tatcgttatt
     1201 ttagatgagc cgatgaacgg tttggatcca caagcaacaa gagatgtgcg aaaaatcatt
     1261 accgacttgg ctactcaggg cacctctttc ttgatctcca gtcacttact cagcgagctt
     1321 gaaaaaatgg tcgatgagct gatcctgatc aatcaaggaa agatcatcaa acaatgtaca
     1381 atggctgaat tgaaccaaac agcgcaagat ttcatcgtta tctctacaac aaatgatcta
     1441 gctgctcgaa cgatcttgac agaagctgga tatactttgg aagatcctga taaaatcaag
     1501 cttttaaaag aaaacgaaga attattagga accgtgatcc agctattgtc tgcacaaaat
     1561 atccatgtga cagacgtcca gcatctgacg aatgatttgg aatcttctct attggaaatt
     1621 ctggattcag taaaggggga agaagaaaaa tgacattgct tgtaaaacaa gaattattta
     1681 aattgatcaa gaaaaagtca actgctgttt tatctgttct attagttgtt ttgctcatag
     1741 gaacagcact tctagccaaa aaatacacga cgatcattga tccagttgaa atgactgctc
     1801 agctttttag tgccacatct tggattgtct ttatcatgat tgcagctgca agcacgatca
     1861 tttcgatgga agcacaatat ggtacattga aaaatctcct ttatcgaaag tattctcgag
     1921 gagaaatatt agtaagcaaa tggatcacat tagttattta ttctgtttat ttataccttt
     1981 tagctattat tgtcactgtg ttgatgaaac tgattctttt cccttcgatt tccttcacgg
     2041 agaaggtttc tactggtcaa acactgatcc aaagcttgtt cacttatact ttgggtagtt
     2101 atatcggttt atggctgatt ttaagtttag tgttgatgat tgcttgcttt atcaatagtt
     2161 ccggcgcatc catctcagca ggaatcgtct tttattttgc ttcgtcaatc atatctggta
     2221 tccttattgc actcatccaa aaatgggagt ggatcaaatg gaatccgatc agtatgttga
     2281 atctgcaaaa ccaaattgga aacgaagaga tcatgaagca attgactcat ctttctacta
     2341 atcaaatgct attcggtaat ttagcttaca tcgttttgtt cttagcatta ggatatctcg
     2401 tatttaagcg aaaaaatgta tgatgactca tcaaaaaaga ggctgtgaaa gagacgttca
     2461 gccttattgc cgattggctg tctcttaaac accgtttatt ctgaaaaagg agctgtgaca
     2521 acatttgtgt cacagctcct ttcctagtat gtttttgaat aaacgacagg ctcacaacct
     2581 ctctttatac ttcttttata cttctatacg tgaagcatcc cagtggcaaa taaaaccatt
     2641 gcaatcactc cgaccatcgt cacacctata agtaaaatct tatctaagtg gatatgttgt
     2701 acaggctcgt tttgctcctt acgtgctttg aggtaaaaat aaatgcctgg caagaaaaga
     2761 acaagaacaa gcattacttc attaaaggct acgactagca tagcccatac ttgaaaggca
     2821 cttgctaaaa gccctactaa aaattggacc cattctttcc gctgataaga aagtttcatt
     2881 tggtacaatc ctaagaaaag ccacgtgaag aaaatagctg ctgtaccaag tgtgtaagca
     2941 aagttataag cactgtcagt gaataataat gaaagtaaaa agacattcgt acataacgtc
     3001 gtcaatacga tagcataagt tggtgcccca tttttgttga gttttcccca tgctttaggc
     3061 aaaagtccgt cttcagccat gtttcccatt gtttcaatcg gtaagatcgt ccatgagagc
     3121 caaacaccaa ctaaagaaat gatcaaaccg ccgttgatca cgactgctcc ccatttacca
     3181 accatcattt caaacacata gcccatagca ggctgcgcct tgatcgtttg cagctgattc
     3241 atcgtcattg tcccatatgg aagcatggaa accaagacgt aaataataat caagccgaat
     3301 atccctagaa tcgttgcttt cccaacatct gattgttttt ttgctcgtga acctacgaca
     3361 gaggctcctt caataccaac aaatacccaa agcatcacca tgatgcaact caatacttga
     3421 ctggaaaacg ggatcggctg tccattcgca ttcaatataa tgtcgctaga aaagttatca
     3481 atgaattgtt ggatcgaaaa cgtgctgata gctactacaa taaataaaaa taatggcact
     3541 aatttacaga atgtgacgat agcattgacg aaactagcat tttcgactcc ccgattcact
     3601 agccatgcat atccccataa aaagactgta ctcatcacga tcgatgccag attttgccca
     3661 ccttcaaata ctgggaagaa atatcctaat gcgctcatca acaatgttgc aaaagctacg
     3721 ttgcctaacc agcatgtgag ccagtaaccc caaccgctga taaaaccagc gaaacggcca
     3781 aaaccttctt ctgcgtaact aaagatcccg ccttttaaat ctggacgttt tttattgata
     3841 ttattgatcg acaaggagag catcaacatt gctgttccta cgagtaccca actgaaaata
     3901 gctgggccgc cagccgatcc ggagatgtcc gtcataatac caaatacgcc acctccaatc
     3961 gctgaaccaa ttactaaggc agcaagtgga attaaaccaa ttcctttttt ctgttgctct
     4021 gtatccatgt aagcatcctc caaaataaaa ccgcttttat caaaaagtat aacaaacctt
     4081 ttttattttt acttaattaa taaaaacttt aatagaaata taattctacc ggcttttttc
     4141 tcattataca agcaaagcgc ctagtccctt tccaaattac accaccaaaa aacttaaaaa
     4201 agatgctata ctaaaaggaa agaattggag gatgatccat gaacatttta tatatatcga
     4261 tttcaggaaa tacgcgtgcc tttgccaaac atctagcaga atatgctgaa aaaatgcatg
     4321 cagaagatcc tgtcaatcct gaagttactt taaaagaaat ccatgaaaat agtgatttta
     4381 cgaaagaaac agaaccattt tttactttcg ttcctactta tttagacggc ggaaatggtt
     4441 tagataacgg tgatacagaa atcttgaccg aaacgatgcg tgaatatctg gaacatgaag
     4501 acaatcataa actttgtctt ggtgtagtcg gtagcggaaa taaaaacttc aataatcagt
     4561 attgtctaac agccaaacaa tatgcacaaa catttggctt tcctttcttg gcagattatg
     4621 aattacgggg aacaccatct gatgtgacac gaatttatca gattttagca gaaaacaata
     4681 aataataaaa aagaagaagc tgaaaaatag atttcagttt cttctttcgt ttctattgtt
     4741 tctatcatat taagctttga catccatcac aggtgctttt gcttttgctt tacctgtttt
     4801 ttccaagaca aagtttttta cacgatccat attggttacc aagacgatca tggatgtttc
     4861 tttgcctgct gctttgatcg cttctaagtc agcttctgct acaagcgtat caggtgtcac
     4921 ttgctgccct tctgttactt tcacatcaaa tggcgtaccg cctaagtcaa ctgtgttgat
     4981 tcccatatga agcaagattt ctaaaccatt tggcgttgtg atcccaatag cgtgtttcgt
     5041 tgggaagaca gagctgacta ccccagtaac tggagaatag atatgtccgt tttctggttc
     5101 tacaaagtag ccttcaccca tcatcttttg tgcaaaaact ggatcacttg ctttatctaa
     5161 agaaacaact gttccgtcag caactgatga gaggatagtc acactttcag ctgaagtttg
     5221 aggtactact ttttcaggca cttcatttac tgtggctgtt tcagtcgcta attcttcttt
     5281 ttctaaacgg tctttaggaa taccgaagaa gaatgtagca acgaagccac cagcataagc
     5341 tgccaataat ccaaggatgt agcctaacca tttaccgtca gagatcaatg ggatcaaagc
     5401 ggctcctgaa ggtccaatag cgatagcccc gacatttccg atcattccga tcactgcccc
     5461 gccgatacct ccaccgatac aagcagtaat aaatgggcgt cctagaggta atgttactcc
     5521 gtaaatcaat ggttcgccga ttcctaggat cccgactggt aaagctcctt tgatcatttc
     5581 tgttaattct ttatcttttt tgcagcgaat ccataatgcc aatgctgcac ccacttgtcc
     5641 tgcaccagcc atcgcaagaa ttggaagcaa tagcgtacga ccagtttcat caatcatttg
     5701 cacatggatt ggagtcaaaa tttgatgcag tccgaacata accatcggca agaataatgt
     5761 gcctagaacg aagccggcaa atgctccacc aacgccaagt acccagttga tcccaccgac
     5821 catgccatca gaaatgaaac ctgctaaagg catgatgaag aaaatttcaa tcagaccgat
     5881 ggcaatcaat gaaagagttg gtgtaacgat gatatctacc gcatcaggta ctactttatg
     5941 cagttttttc tcaacgattg acaatagcca aacagcgaag atgacaccta ggatcccacc
     6001 ttgtccagct gcaagagctt caccagtaaa taagtttttc agtggtgctt ccggattcat
     6061 ccctgtcagc atcacaacgg ccccgataac tccaccaaga gttggtgttg caccaaaaac
     6121 ttgtgcagca ttgatacctg tatagataac taggtagcta aacatcccgt ttttcaagat
     6181 gttcatcaca tcgatatatt gttgccatgt tgctgcatca agtgttccgg ctgtgactaa
     6241 gtttgctaaa actgcggcaa tccctgccac gatcccagta ccgacaaatg caggaatcat
     6301 tggcacaaag atattagaaa tgtcttttaa taccgatttg attttagatg gtttttgttt
     6361 cgatttttgt gccgctttca tttcttgtgc ccgttcgttt acacggtctt ttccagatgc
     6421 tgatgaattt gctgtagctg tacttacaga agaaagtttt tctcctaatt tcacgcctgc
     6481 caaatcaacc atttgctgtg ctactttgtt cactttccca ggtccgatga tgacttgaag
     6541 ctgttcatct tctactacac caagcactcc tggaatcgct tttaattcag agacatttac
     6601 aagatcatca ttgatgatct tcatacgcac acgagtcatg cagtggacaa tgctttctac
     6661 attgcccatg ccgcctgccc ctttataaat ttgttttgcc agttcttcca aagtcagttc
     6721 gttttttgcc atggtctttt ccccctcata aggtcccttt tacaaatccg tttgcttcag
     6781 ctaattttgc ttgagcttcc tctttatcac tatttgtcaa aatcatgacg atagccagct
     6841 taacattttg atctgcttct tcaaagtact tttcagctgt ttcgtaatca gcttctgtcg
     6901 cttgcatgat gattcgtttt gagcgttcga ttagtttttc atttgttggt ttgacatcga
     6961 ccatcaagtt attgaatact ttacctatac caatcatact gatagtagaa atcatattca
     7021 agataagttt ttgtgctgtt cctgacttca atcgagtaga gcctgtcaaa aattctggac
     7081 ctgcatcgac ttcgattggg aaatccgcgt gtttactgat ttcggcttgt ttattgcaag
     7141 aaatcgttcc tgtgacagca ccgatcgatt ttgcgtaatc taatccaccg atcacatatg
     7201 gtgtacgacc gctagctgcg ataccaataa ctatatcatt ttttgtcaaa ttcagcttga
     7261 ttagatcttc ttgccctagc tcataagaat cttcggcccc ttcgacagct acagtcattg
     7321 cttcttctcc accagcaatt agacctatga ccatttccgg ttctacaccg aatgtcggta
     7381 cgcattcagc agcatctaac acacctagtc ttccacttgt tccggctccc atataaatca
     7441 gacgtccacc ttttttaaag gactcaatcg tgttagcaat cactggttcg atacttgtca
     7501 gttctttttc aaccgcagcg gccactttct gatcttcttt gttcattagt tgaacggctt
     7561 gagaaatact catctcatct aatccaaaag tttcttggtt tctgcgttcc gttgttaggt
     7621 tctctaaatt gattttgctc atttttatct tccttctttt cttataaatt gatagttcat
     7681 accagctttg atttggttga tcaatggtag ctcttctttg ataactttag ctactacatt
     7741 cactttttca tccgctggca aatcatattt ggttaattgg atctcaccca tatagcgcaa
     7801 atacttttcg ttatctaaag taatagaccc tttcatacgc gtagctgtat ttcttgctgg
     7861 gataggcgga atctctcgaa aacgtgcatc tacgcttcgt atgacatctc ttgcatcgtc
     7921 ttgccgattc gtatgcttcc ctaatatata ttcgtaaaat tcttcatcta caggttccgt
     7981 atggagcaag actgtctgtt ctttttggaa agaagcaaat tgctcctgaa cgttttctga
     8041 cagacctgca tctccgatgt aaacaatatc cgtgttactt gctaatagat ctagagcgcc
     8101 tgcaagagga tggatccctc gatgttcttc caaagttggc aatccttgat atagtgggcc
     8161 tctcaactct gtatctcctg gtacaaaacc ttggacaagg agaccttgaa ttttgagcca
     8221 ttgatttttt ttctgatacc attctttgtc cagccctgtt tcgggacgtg gataataatt
     8281 atgccaagct tcaatctgtg agaaatcagc ttctgcttct ttcaattcat cgatatcttt
     8341 aggcgtgatc gtgctagcat tcaaactgat tttcaattga tgtgaccagt cggcaatctg
     8401 ctgattggaa atatgataat ccattcttaa acctgttaca ccaattgctt tcaaaggcgt
     8461 tagatcttct aatgaaaaac ccgctcgttc caatgcctct cctgaaatat cgaccatcaa
     8521 ttctagttta ttctttttcg cttcgcttcc tagttcgatc aatcgctttt gatatgccga
     8581 aacatcatct tctggtatat gcatggaagt gaaaataccg acaaagccat tggatgccat
     8641 ttggcggata tactgtttct tctcttctga caaagaatcg ttcataaata ttgaaaagcc
     8701 aaacatatta tccctctttt tctgtatccg tcttcatttc tatgactaga gtatatatct
     8761 aaagaaacaa aatttcaata cttttattaa aataattaaa tttcatttct aaaggttgag
     8821 tttatattat caaaagaatt gtcataacct aaaaaaggca ctgctaagca aaagcagtgc
     8881 cttgaaatat tttatttaaa taacacaaaa acataattac tcataagaaa ccaattctgt
     8941 attatctacc atgaatgtaa agaacccctc attaggcatc ggatgaaaat aagcattgat
     9001 cactcttatc aaggcattat ggcaaactaa cagatagaca ttttcttcgt cttcgatgca
     9061 ttctttcaat aaaggaacga tccgtgcata cacatctaag actgactccc catttgggaa
     9121 tcgtacagcg aacgctaaac gtgctttttg aaagttctca tctttgcttg gcagtccatc
     9181 ataatcccca aaatccattt ctaccaatcg ttcatcaacg gtcagcggca agctgagacg
     9241 atcagcaact gcttgtgctg tatctcttgc tcgttgaaga ggggaatgga tgattttcgt
     9301 gattggaaca ggcaaatcta ctaccttttc tgctagtttc tgtgcctgca tctgtccttt
     9361 ttcagtcagc ggcacatctg cccgtccgca aactaatcct tgtgcgttcc aagttgtttc
     9421 tccgtgtctc gttacataaa gcatgtgatt ctctccttcg ttggcaaagt tccccttaac
     9481 cataccttgt tgttcttgag attacaaggg ttagattctc tcaagagaaa taatctcagt
     9541 agatacttta tccaggaagg tttgatcatg ttccactagt aacatggcag gtttcacgtt
     9601 caagatcagt tgttctaatt gttcttgatt gaagacatcc aaataattca atggttcatc
     9661 ccatgtatat agttcagctg gctgtgataa agatttagcc aattccactt ttttccgttg
     9721 gcccatactc atctgctcga tcttgttatg aaaaacatct ctttccatcc caagctttcg
     9781 gaggttgttc aaaaatgctt ggtagtctac ttggtttttc tctgcaaatt ccgccaacgt
     9841 tcctcgattg tcttcataat tttggcttgc ataactaatg ctcagatgtt ttggctggta
     9901 tttttctcct ataaccttgc cgttgaatgc ccccagaaga taatggataa tggatgactt
     9961 tcctgcaccg ttaggacctg aaatcgccac ccgctgatga ggctcgattg taaaatgaat
    10021 tggctcgaat aacagatttt cataccctaa ttgaagatct tctacgctta aaagtcgctt
    10081 atggtgagac gcttggctat tcatcgtcaa agaatcgata tattcgatat cttttaatag
    10141 tttttctttc tcgctgatct gggtctccat ccgattcacg attgctttgg atcgtttcat
    10201 cgtccgtgca gcatcagcac ccactgctcc tttattcact cgtctagatt cagtatcgat
    10261 gaatccgact tgtttctttg ttttatctcc ttctcgggaa cgagaccatt cggctttttc
    10321 agctgctgtt ttctttagcc tactgacttc ttttttcaat ttttcatttt gagccatttc
    10381 aaattcatca cgaagttttt tctgttcttc atagatagag aaattccctt gataaagttc
    10441 cagttgactt ttttcgattg ctaaaacatg gtccactact tcatcgataa atccccggtc
    10501 atggctgacc acgatgaagc cttgtttttt ctttttcaaa taagccgcta cttgttttct
    10561 accagagata tccaaatgat tcgttggttc atcgattaac gggaaatgag tgtcatccac
    10621 aaataaaagt gccagtagga cttttgtctt ctctcccccc gatagtgtgc tgaattctct
    10681 ccataagatt tcaggatctg tttgcatcaa ttggagctct ctttcgattt cccatatctc
    10741 aaaatccgta atatcattta acacgtaata ggttaaacgt tctttatctt ttgtctgttg
    10801 cgggaaatag gcaaattctt gctgatggat tacttgccct tgataaggta gtttgttttg
    10861 tagaatattc agtaaagttg tctttcctcg accgtttcgt ccgataagtc ctagtttcca
    10921 ttgtgtgtca aaatttagat ttgcttgttc aaataataat gtgccttggc tgtcgtagcc
    10981 gaatgtcaga ttttttattt cgattttaga cattttccat cacctcgtaa aaaaagacca
    11041 gtcctatttt cagaactggc taaagaggat gcaagggttg gatacacaca aaaagaccca
    11101 tacatcgaaa aaaccaagcc aatcccgaaa atctgcacac tgcttccttg tctccacaag
    11161 gtacagtctg aatgcagctt tcatcaatcg acttaatttt tcaatgcacg gatccctcac
    11221 tttgtcctat atttgaaatt acgatagcat aaagaaaatg attttgtcaa ctacttcggg
    11281 aggatggtga aaaatctgct aagtgtctgt cctaagattt ttgttttttc taatgtttgc
    11341 ctgtgttcca ttgcttcttc taaacctatt ggacccaaat gaatacttaa gacaattggg
    11401 agaaaatcag aaagtccttc tagctcttgg tctctacttc cgcaaatagc gattattggt
    11461 tttcgatatt tttttgcaag cactgctaca ccgtatggaa cttttccgtg gatcgtttgc
    11521 tgatcgatcc gcccttctcc tgtaatgatg acatctgctt gttcgatctt tttttccaat
    11581 ccagtgattt ggctgactaa atcgaaacca gaaaccatct tcgctcccaa caaaaaaagg
    11641 gctccaccaa taccgcctgc tgctccactt cctggaatat catctaaatc cacctcccag
    11701 aaatctttta tttgctgagc aatctttcct gcttgtttat ctaagaaagc tagcgtcgta
    11761 tctattcctc ctttttgtct tccaaattgc caagcagctc cttgttgacc tgtatatgga
    11821 ttggtaacat ctgcagcgat aatcaatttt actttagaaa caagaacgtt tcttcgatca
    11881 aaatgaagtt ttttagtaga taataaagga tttccttctg tgtacccttc taaagaagca
    11941 cccaaggatt ggagtaatcc tagaccacca tcgctacacc cgcttcctcc taaagtgaca
    12001 ataatttgtt ctacttcttg aagcagtgca tctttaataa gttctcctaa ggcataactg
    12061 gaagcaagtc ggatagtttg atccgttggt ttatcaagta gatctaaccc caatgcttgt
    12121 gcagattcga tgactgcggt gttttttcct tgccaagttg tcaataaata gctgattttt
    12181 cgctgacgga ataagagatc catcccatca atggtacgcc atgttcccct gagcgcgtaa
    12241 tagattgctt ctgcagtatt ttctccgcca tctgcaatcg gcacattgat ttttcttcct
    12301 ttacgatctt cccagccatt tagaactgcc tgatttagtt caaatgaaga agcacttcct
    12361 ttaaatgaat caattgccgc caaaatatcc atctgtatcc ctcactttct tcttaagtaa
    12421 gacacttttt ttcatttttt tcaaacaaaa caatgctatt ttcttccatt tttctatata
    12481 ctaaaaggga actcattgaa taaaggagag aaaaaacatg aaaatcgcaa tagctggggc
    12541 cggagcaatg ggcagccgaa tcggattgat gctacatcaa agcggaaatg aggtcctctt
    12601 gatcgatcgt tggcctgctc acattgaagc cattcgtaca aatgggttga ttgctgactt
    12661 taacggtaaa gaagtggtcg caaaactgcc aatctattca ccagaagaaa tcatcgaaag
    12721 caacgaacac gtggacttga tcgtagcatt aacaaaagcc aatcaattag atgatatgtt
    12781 ttgttcgatc caatccatta ttactgacaa tacttatgtt ttgtgtttac taaatggatt
    12841 aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa aatatcttat ttgggatcac
    12901 gatgtggaca gctggtctag ctggtcccgg aaaagtcaca ttgttaggtg atggagaaat
    12961 cgaactagag aacttggaac cagaaggcga agcatttaca aaaaaagttg tagaagtctt
    13021 tcaagaagca aatttaaatc cgatctatag ccataacgtt cgttattcca tttggcgaaa
    13081 agcttgtgta aatggaacat tgaatggttt atgcacgatt ttggattgta atattgctga
    13141 actaggcgcg caaaaagcgg cagagagcat ggttcgaaca atcgtatctg aatttgcttc
    13201 tatcgcagca aaagaaggaa tcatactaga tcaagaagaa gtgtaccaac atattgcttc
    13261 tacttatgat cctgacaaca tcgggttgca ttacccatct atgtaccaag acctgatcaa
    13321 aaaccatcgt ttgactgaaa tcgactacat caatggtgcc atttggcgaa aaggacaaaa
    13381 atatgatatt gctacaccat attgtgcctt tttgacccaa ttagtccatg caaaagaagg
    13441 aatccttggc gcagaataaa aggctctctg atgagaatca gctgaatcag cagctggttc
    13501 tcttttttta agcccaataa aagatcaaaa agaaaacaca actctcatga tgccataaga
    13561 attgtgcttg tttgtttcag tacataatct cttcaaaatg taggtttttt atttgttttc
    13621 aacagaggag aggtcatatt cgtattgctc ataaatagtg gaatcgttag tgataaatcg
    13681 ttttgcccaa ttttctgcat cataaaattc caatagctct gataggattg gcttaaatgg
    13741 tgtatgcaga aattccggta attcatctaa atcacgttct cgaagcaaaa aaatattttc
    13801 aggacgataa aaatgataat gagcaatcgc ttgattatcc gtcactagtt tcttttgaaa
    13861 atacatggat tcaaatgtcc gcaaagtcac tccttgctga ccctcccgca acagttccag
    13921 aatcccgcgc gattcccctg tttttttcag gtactcttca taagtaagat atccaggttg
    13981 ttcattccct cgatgtggaa gaatgaaata gttggtcgta acatctcttt ttataaattc
    14041 ctgcttatag ctttctgctc gttcttttct cccattattc gttgcaccaa aaaatagatc
    14101 cgtctttggt tcgctagaag aaaaattcat atgtttcgaa taaaatgaac tattatgttt
    14161 cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa atctcatcga ttgccggttc
    14221 atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg aagtgattcc aaaaaaataa
    14281 cacaattttt gcattggtaa atgggcgcac gcgtcgagcc atctccatat caaatccata
    14341 atctaaaaaa acgatcagct gataatttgc caactgttgt ctagctgatt taatgaattc
    14401 cgactgtagt aaatcttttc cggaccaaac atctaaagag cggatggaag gcaaaaaata
    14461 aagtgattta ttttttgcaa ctagcagtac gtcatttttg ttcatctttt ccgcctcact
    14521 ttctctgtca ctttttccta tgatagcctg tttaaatagt aaaaagccag taaaacgttt
    14581 cctatatttg tatgaaaatt taacatttgt atgaaaatat gatgaaataa atcattcttc
    14641 tattaaaatt cgcctaaaag ttttctgaat agttgtaatt gtctgaaaat ttgttatcat
    14701 tagtcattgt taaatattgc tatttattct taatataaat tttgattgtg aatttaaaag
    14761 aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa cttagctcca gacatatcac
    14821 catgttagct ttaggtggag caatcggtgc aggtctattt aaaggaagtg gcgaagcgat
    14881 tgggatcgcc ggcccttctg tattgattgc ttttttgatt ggtgggctga ttttatatat
    14941 cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc gacacgcatc acggattatc
    15001 tggtttgata cgcccttatc taggtgctca ttctgctgat tttactgatt gggtctactg
    15061 gtctatgtgg attatcaata tcatcgctga agctgttgca gctgcttcct ttttgcagtt
    15121 gtggttcccg cacattccag cctgggtatt tgtttttatg ttggctgttt taactacgat
    15181 catcaatctt tattcggtac ggttattcgc agaaacagaa tattggctgg cttttgctaa
    15241 aatcagtgtc attatcttac tgatcatttt tgctacttat cttgtcggtc agcaaatgtt
    15301 gggcacaggc gttttcccta cccttcagca attgactgac catggtgggt ttactcctca
    15361 tggtatgaag ggaatcatca gttctttgct tgtagttatc tattcttacg gcggttctga
    15421 actgattgct atcacggtaa gcgaagcaga tgatcctaaa cgtgcgattc ctaaagcaat
    15481 caaaggtgtc atgggaagaa tcatttcctt ttacatcatt cctttgtttt tgcttttgat
    15541 tctttttcct tggaatactt tagcaggcac gaatgtcagt ccttttgtga tggtatttga
    15601 aaaaatgaat atcccatttg cagcggatat cgtcaatttt gtgattgtcc ttgctttgtt
    15661 ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt ttgttcttcc gtttgaagga
    15721 tcgaaaaggt tcctctagaa aactagctgt tttaaacaag catcaagtac cgcaacgtgc
    15781 tgttttattt tgtaccagtg ttctttatct tggtgtagct ctttcttatt ttgtcggtga
    15841 caaattattt ggctatctgg caggttccct ttcgtataca gtattattga tctggatcat
    15901 tatcagtgcg gcatcctttg tactggcact aagaaaagga acattatggg ataaaggaat
    15961 cagctttttt gcattagctg tcttaggatt gattttcctt ggtatcctgt tcacgaactc
    16021 cattggggta actgtattga ctggcctgct ttacctgttc atttatttca gttatcaaaa
    16081 aaagaatgat gcgttcgtat tgacgaatga atgaaatcga tagaaaattt tattttattt
    16141 atgtggaata gatggagcta ttgaagaaag gagttgtgac aaaagtcttg ttgcaactcc
    16201 tttttccgaa taaacagtct catgacctct tggtaaacgg tgttcaaaaa ccctctgtgc
    16261 aaaataattg attgttctcc atgctcatga catgatagaa gaaaggaatg aaaaaaagga
    16321 gtttgtttta aatgaaaaag tattggaagt ttttgttgat gatcggtgtt tcaacggtag
    16381 ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga tcatatcttc ttcagtcgta
    16441 cacgagtatt catggcgttg atgatgggtg cagtcatggc agtcatcatg ctgttgttca
    16501 tgtggaaaat gtatgaaaac aaaaagataa atgttatgat cttagccgct agtcttcttg
    16561 tttttgccgg ttccttgttt atggtacgaa gccaaactac tgtcaacgac acagcttgga
    16621 tgaaggccat gattcctcat cattctatcg ctatcctggc aagtaaacga gctgatttaa
    16681 aagatcctga agtaaaaaaa ttagcggaag aaatcattca ggcacaagaa gaagagatcg
    16741 acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt atccgtccaa ataaaaaaag
    16801 aggatgttcc agaagttatc agcttcaaga aataagaaga agctacttct ggaacgccgt
    16861 gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat aagaacagcc aaacaaaaat
    16921 agcttctcct atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc
    16981 gtttattaag aggttgtaaa atcagcgttc tgacctgagt attagagaga caaatggaaa
    17041 aatagttcct catattttgc cattttttga gctaataccg agggtcagct gattgaacac
    17101 cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg tattaagaaa aattttgtga
    17161 aatggctttc cacatttttg tataaatttg gcttaatacc gagaaactgg cttttgaata
    17221 ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca caatcccttt ttatgttttt
    17281 attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg tttcacaaat ttttttgaga
    17341 ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg agaaagcaaa ctgaaaaata
    17401 cgaatggcaa gtaagtgagt gtaggaatac caaatgtact tgctacgaaa cttccggcaa
    17461 ttccccaagg cactaagtag ttgattactg ttccaccatc ttctagcgtt ctacctaata
    17521 cagttggatc aaatcctgct ttttcataag tttctttaaa tgcattacca ggcagaataa
    17581 cagataggaa ttgttcacca ataaaaatat ttactccgat acttgtcatc aatgttgtaa
    17641 aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg ttccatcacg gttgagatca
    17701 agccaaattc gatcaacaat ccacctaatg acaaagttag aataatcaat gacacagtag
    17761 gcatcatact ttcgattcct ccgcggctaa gcagtgaatc gatttgttgg tttccagtat
    17821 ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt taaggaagtt tgtgggtttt
    17881 gaataaaaat cattcctact gctacagcga tattcaacaa aatcgttaaa attgccggca
    17941 cctttttcca tgcacacagt aacattaaaa ataaaggaat cagtgaccag attgaaatag
    18001 taaagtttgc ttctagaaca tcagtcactt cacgaataga tgaaagactt gcggctttac
    18061 ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac aaatgccggg acagttgacc
    18121 acatcatatg cttgatgtgt ttgaacaaat cggcatcaac aatagctgct gctagatttg
    18181 ttgattcgga taatggagac atcttatctc caaaaacggc acctgaaaca atagctccaa
    18241 caaccagcgc tggatttaag cctagtgttg tcccaatacc gaaaaatgca atcccaatcg
    18301 ttgacataac agtaaaggca ctccctacag cgctacctac gattgcacac acaacaaata
    18361 cagaagggac aaaccactgg acactgatca atttgaaccc aatgaccatt agagttggaa
    18421 tgatccctgc ttgtatccat acagcaatca atgaaccgac taaaataaaa ataaagattg
    18481 ggatgattcc tggtttgatg ccattgatga ttccttcgtg cagccaatct gtcgggtatc
    18541 tttttatcac tgcatacaac atgaccattc cgatcgctgc taagatcgtg atattaggtg
    18601 agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac aataaccatt aatacaagtg
    18661 ttgcttctaa aaaacttggt gttccttttt ttcccatgat aatctctcct tttttagttg
    18721 ttcttatgtt ctaaaaaagt taagatatcg gattactccg ccgcaattca tccgcgattc
    18781 ctcctttata aaaataaaaa gacctgtttt aaactaagga cgaatgactc cgcggtacca
    18841 ccttaattaa aaacagatct gtttttcact ttcaaacttt tattcaaatg tatggacttt
    18901 ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga ccaccgactt tctgaacaaa
    18961 acacctaacc gctactgcgt gaaaagttga cttgtacgaa gtttaccttc cataagatca
    19021 tttgtcaatg aaaaagaaaa tattttttca tttttctgaa aattattttt ataactttca
    19081 cacacaagtt tattttttac tagaaagtag cgccctcttg tttttttctt gctactattt
    19141 cttgatgttc ttgcttgatt ctttctaata gctttggctc tttcaacaga tcaagagctg
    19201 tattggcgag tagttctgct gcaatcgcaa tagaagcaag gcctttgtca cttttagcag
    19261 cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc agagattgct accgtaggct
    19321 ggattgttgg tatgacttgg ctgacatttc ctacatcaga agaaccgaga cttgttcttt
    19381 gttccgtttc tatttcttca tctggaacac ctgcttcttt tgcatgctgg aagaataagt
    19441 catcaaaaga aggagtaacg atgacatcat caactgcatt ttggaacaat ccaaactcaa
    19501 aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt ttccactttt tcatatactt
    19561 tatctaatgt ttgccgattt cctgcacgta aatagaaacg tccagcagcg taatctggaa
    19621 tgacgttggc agctactcct ccgtctgtaa tgatcccatg gatattgaca tcttttggca
    19681 attgcaagcg tagagcgttg attccattaa acacttggat caatgcttcc aaagcattta
    19741 ctcctttttc aggtgctgct gctgcatggg aagcgacacc gtaaaatttg atgtcgactg
    19801 gatcattcgc taaagattct gtcgttttcc cataacgaaa agctggatgg acgcataacg
    19861 ctacatcgac atcctcaaaa aaaccttctc gaacaaagct gcctttagct gatccgttct
    19921 cgcccccttc ttcccctggg gtgccatata cacggatttc tccaccagtt tcatcaatga
    19981 attgcttcac tatgctggcg gctaatacgg agtaagtacc aaacaaatta tgcccgcatg
    20041 catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa ggcaattgtg ggaccgggtt
    20101 tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc agctacttct ttttctaccc
    20161 aaaacctttc tttttctaat tgtttaatca acgctgctga ggaataaaac tcatagttac
    20221 ttacttcagg atgttcgtgg atatctagag caagtgcttg atattctggt aaacgatatt
    20281 ggattagatc cttcaattgg tttgttgaga ttgttgttgt cataaaaaat tcctcctatg
    20341 ctagtcattt ttcttatttt ctcccgaatg tttcccatgc aggcacgctt gatccttttg
    20401 atgttttctc gattactttg actgtgtctt ctgtttgata agcgtctact attttttgat
    20461 acgttttatt gttttcatct tcttttcttg caacgatgat attcacataa ggacgtgcat
    20521 ggtcatccac tggttctaag aagatggcat cagaagcagg attaaatcca gcatctacag
    20581 ctacaccgct attgatcact gaaatgtcta catctgacaa agcacgtgct gtttgggaag
    20641 catctaattc agaaatgtct agattttttc tattctcggt aatatcagaa acagtgggcg
    20701 cttgtccttt tgccttatcc actttgatca atccagcagt ttgcaataac agaagagctc
    20761 tgccgccatt tgtaacatca ttagggattg ctacttttgc tccgtctttg atatcagcta
    20821 catccttcaa agtgttagaa taaattccca gcggtgcatt gacagtattt ccgatcgata
    20881 ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa gaattgatgt tggaaagaat
    20941 tcaaatcaat cgatccatct gctaaagcca catttggttg attgtaatct gtaaatttca
    21001 cgtattccaa attaatgttc tcctttttca aacggtcttt cacgttgtcc catacatctg
    21061 tatcttctcc aataatccct agttttacga tagtttcttt tttgtcagtt gtatctgctt
    21121 ttccttggct acaccctgcc aaccaaataa ttgctacaaa tgtaatgact acaaaacgaa
    21181 taattttttt cattttttct cctccacttt caatgtgtaa tttttttaat cagttgatta
    21241 ctgataaatt gacttaaaaa aactaagatt agaatcaata atgtagccac aatcgttaca
    21301 tctgtttgga atcgattata ccctctcgat atagcaagat tccctaatcc gcctgcacct
    21361 actgctccag ccatggtagt cagtccgatc acgttgatga tagtcaacgc cgagacccga
    21421 atgataccag gcaaaccttc taacaaatac acacgtaaaa tgataccgat tggactggtt
    21481 cccatcgctt ctgccgcttc gatcacccca ggatcaactt ctagcagtgc gttctcgatt
    21541 tgacgagcaa aaaacggaac aactccaaaa atcaatggca ctaaagctgc tttcgcacca
    21601 atcgtcgttc caactaagaa acgagtagtc aatgctaaaa gagaaagcaa gataatgaaa
    21661 gggatggaac gaatgatatt gatcagctta tcaagtattt caaatagcag acggttagct
    21721 agtaatccat tagggcgtgt cactactaac gtcacaccta agatgattcc aatcatcccg
    21781 ccaatgaatg ctgtccaaaa agacatatat agtgtttgct tcgtcgcttc gacaaattca
    21841 tccggaatca aataaacatt gggcaaatat gttttaaaga aatcagtcat tttatcttcc
    21901 tccttcgatc actttgatac gactatctaa atcgtttttt tgtaaaatat ttatttttac
    21961 accagatttg atcaaataat ccaatgcttt tttccgctgt ttggtttctc cagctaaagt
    22021 aacgatcaaa ctccctaacg gcgtttcttg taataactct acattgccat ataaaatatt
    22081 cgctgttact tgatatttgc tgtacagatg agcgattaga ggttcgttcg tctgatttcc
    22141 aatataactt aattccacca gccatttatt ggcgatctga tcggcaaaag cagaatgggc
    22201 aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt cgaataaaat ctttggtcaa
    22261 ttcttcttca ggattgctga agatctggat gctgctacct ttttcaacga ttcttccatc
    22321 ttccataacc gctaccttgt tgcagatttc tttgacaact tgcatctcat gagtgatcag
    22381 gacgattgtt aaacctaact ggcgattcaa tttttttaac aatgcaagga tctgaagggt
    22441 cgttttcgga tcaagtgcac tggttgcttc atcacataat aataccttag gttcattcgc
    22501 caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc gacagttgtt taggataagc
    22561 agatgctttt tcttccaaac cgacaagttc cagtagttca ttcacttttt gacgacgttc
    22621 ctgtttcgat tttccagaat actttaaaga aaaatcaacg ttgtcaaaaa tagttcggct
    22681 ttccattaga ttgaaatgct gaaagatcat accgatggcc tttctttctt ttcgcaattc
    22741 tttgggagag agctgactca gattcgtctg attgacaatg acttctcctg ctgttggttt
    22801 ctgcagcaaa ttcatcacac gaactaacgt acttttccct gcacctgagt aaccgacgat
    22861 tccataaaca tcgccttttt caatcgttaa atctacagaa tctactgcct gtatctcttt
    22921 ttctttttgt ttgaaacata cagaaacatc tcgcaattca atcattaaaa ttcctcctta
    22981 tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt cttgaggacg cttttttgcg
    23041 tgttaccacc tcatgttcaa gctgtgttca cacacaactc ctcttcaagt acgatcatac
    23101 tctagcaaga tatcggtcgc agccgtcatc gccttacatc tatgctcggg atgaattgct
    23161 cgatggccat tttccaatag tcgttccttg cttcttctca gctaccgaag ctctctgtca
    23221 aaggttcttc tatcgtactt accatttcaa tgcaaataat attcaaaaaa aaagtccttt
    23281 tactaacata aactatgtca gtaaaaggac gacgtatcgc ggtaccacct tttttcggaa
    23341 gtttccttcc ctcaagccca acatctcaaa aagagagatt ggcgttcgtt ttaacggtcg
    23401 aacagttccg aatttgccta cttatcgaca aattattctc caaggccatt ttcagctttt
    23461 cttacttgac tcttctcacc agccagagcc tctctgcaaa gtttccaaac ttactttcct
    23521 tttcaacgaa atcgatattt tgttgttggg attaatctac atgaggaaaa ctgaaaagtc
    23581 aagtacgaaa ttcaaattat tttttctata tttatttttc tgttttttta tttaacattt
    23641 caataacagt tcgaagatct tttccttccg caagaagttt caatttttcc ttttcttcga
    23701 cggtataatt tcggacaaaa ctggaatcag gtatcaaaac aacgcctaat cctgctcgtg
    23761 aagcagctgt tgctcctact aaagaatctt ccactgcaaa ggctgaacta gcttctaatc
    23821 ctgttcgttc caaagctgtc agataaggaa tcggtgaagg cttgttctcc gacacttcgt
    23881 ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa gtgatccaag atatctgttg
    23941 ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat atgacgttct tttaagtctg
    24001 ccaatatttc ttttgcatat ggttttaagt ggatggctcc gcttgtcagc tgttgataaa
    24061 aatattcttc acgcttcgct cggacttcct gtactttttc agggctacct gctgctttca
    24121 ctaaaatatc aaaactttgt ttccagtttt ttccgcccca agcgtcaacg atttcttttg
    24181 ggatatggat atcaaattgc tgaaatcccc aaagccagcc atcacgatac gttttttctg
    24241 tgtcaactaa caaaccatct aaatcaaata ttgcaccttt catctataca ttaatccttt
    24301 ccaaaacaat acacatagtt tagcacgact ggtccaaaaa gaacagaagc aaagaaccag
    24361 ccttctcgtt tataagagtt cttggaatcg acggatatag attttcttca cagctgtcac
    24421 aagcgccaga taagcgatga ctgtagcaaa taaccataac cagtaattgc caggaagtgg
    24481 catcaagcct aattcagcac caaaacttgt aaaaggaagg acagaaccga caataattcc
    24541 taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt tggataaatg gtactttcgg
    24601 tgtacgcagt gcatggagca ctaaggtttg tgtccatagt gattcgacaa accatcctgc
    24661 atggaataat gcgatgaaaa tcactcgctg tgaactatcc aatgtatgga aacttccgcc
    24721 cactacagca ggacaaataa caaaatacat caacaagtaa gtagtaatat caaatatcga
    24781 acttgttgga ccaaagtaaa ccatgaattc cccaatacta gatgcattcc atttttttgg
    24841 ctcgtccaaa tattctttat ccatccgatc ccatggcaag gaaatgcagg aaacatcata
    24901 gatcagattc aaaaacaaaa gttgtaaagg cagcattggt aaaaatggca ggaacgtact
    24961 tgcaatcagc acggaaaaca tattgccaaa attagaactt gctgtagcct tgatatattt
    25021 catgatattc ccaaaagttt ctcgtccaga aagaaggcct ctttccaaaa tcgtcaaatc
    25081 tttttctaaa aggatcacgt ctgcagattc tttcgcaata tccacagctg tatctacaga
    25141 aataccgata tcagccactt tcattgcagg agcgtcattg ataccatctc ctaaaaatcc
    25201 gacagtatga ccatttttac gtaaagctgt cgtcaaacgt gctttttgct gaggattcaa
    25261 ttttacgaat acttcatggt cttcagctac ttgtgctaat tcatgatcac tcatttccga
    25321 gattttctca ccggtaacca attcattgat ctctagacct acttgacggc acactgaacg
    25381 tgtaactaat tcgttgtccc cagtcagaac ttttacagct acaccatgtt ctttcagagc
    25441 tttcaacgct tgtttcgttg tttcttttgg tggatcaagg aaagcaagat aaccaatcaa
    25501 aaccatgtct gactcatctt tgactgaaaa ttctccaacg acgcttggat tggtcttttg
    25561 tgcgacacca atcactcgta agccatcttc attaagtgac tgtacttttg caagtacggc
    25621 ttcttttcgt tcttttgtta atggtgtaac agtaccgcca agatcaataa atttagaaat
    25681 gttcagcatt tcttcaatcg cgccttttgt gatcatttgt gttttaccag ctggatcagc
    25741 aaccacaaca ctcatacgac gacgttcaaa atcaaaagga atttcatcca ctttttgata
    25801 attgatctta tctgtatcta aagtttctgt tgcttcatca atgatagcca catctaacaa
    25861 atttttcagc cctgtttgat agtagctatt tagatacgca tggcgcaaaa cacgatcatc
    25921 ttcatttcct tcacaatcca tatgatattc aagaatgatt ttatcttgtg tcaatgtccc
    25981 cgttttatcc gtacataaca catcgattgc accaaagttc tggatggcat tcaaattttt
    26041 gatcactgtt ccttttttgg ccatcacact tgctcctttg accaaattcg tcgtaacgat
    26101 cattggaagc atttcaggtg ttagaccaac tgctacagat agtccaaaaa gaaaagcttc
    26161 tagccaatcc ccttttgtaa gcccattgat gacgataact gctggagcca tcaatgacat
    26221 aaatttgatc agtagcatgg atgttttatg gatgcctaat tcaaagcttg attgaattgg
    26281 tttttcagat agcgtttctg ctacatgacc aaatagtgtt tcatttccaa cagaaacaat
    26341 gattccttcc gcacttccac tgatgacatt acttcccata aagacaagat tttcgtaact
    26401 tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc ttttctactg gatagctttc
    26461 ccctgtcaat gctgcttggg aaatgaataa atcctttgcg gacaataaac gcatatccgc
    26521 tggtatcata tcccctgctg aaagccgaac aagatctcca cagacgattt cttccatagg
    26581 aatctctgtg tactttccat tacgttttac agctgccgtt actttcacaa gtgatttcag
    26641 tttttcagcc gcttgattgg aacgtacgga ctgaatcaac gtcattgtcc cgctgacgat
    26701 aaccatagtg aagataatgg ctacaccgaa aagatcttta tctccaggag ctgctaatac
    26761 atattccgtg atgaatgaaa taattcctaa tgcgatcaac actaatgtaa atggcgtgat
    26821 gtatgctttc aatatttcta caatgaatgg tgtttttttg ccatatgaaa tcgcattttc
    26881 accgtaattt tctcttgaac gttcgatttg ttcttctgtc aatccatttg ctgatgcatg
    26941 gaatagttca aataattctt gtttcgtttt tccagcaaaa gctgcataga ttttttgtgt
    27001 atctttcttt ttttccatct caatctctcc tttgtgtcca aaagacagct gttctcgctg
    27061 cttttggtct ttagctattc atcggatcga tagagaaaaa actgaaaggg gctgagaaca
    27121 gatagataat agccctggcc tttcctctag atccttgttt ttcgaatgac tgtcatccat
    27181 ttgttctcat ctcctttact aagtaatttt aattcatgac ttccaatcat ttttcgaata
    27241 gttatttttc caattagacc acctcctttt tatttttcac cgatggggat ttgcggcaac
    27301 aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa aagcatatta aaaaaagctt
    27361 ctcagatctc atcgttgagt tttgacactg tacaacgtaa aaagcagata cctgctttta
    27421 gctaccttat gaaaagcctt atctcgacaa ttcctgttct acccattggc gtctctcgac
    27481 gtttttgggc agtagcctat gtttgtatag gagcctcacc taacaaatct tattcggttt
    27541 tccttcgtta atataacaac taaaaaagag gatgtcaaat caattttcca tgtttttcat
    27601 acttttttta aaatacacac gttatattcc tgtctcacgc ggttttattt gtctcatttt
    27661 ccacctcccg ctataataga aatatagtag gaaacgaggt gctgttatgg aaagaacact
    27721 tgttattatc aagccagacg gcgtgagacg ccatttagtc ggaagcatca tccaacgttt
    27781 tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa actatgactc ctgaattagc
    27841 aaaagaacat tatcagcact tgactgagcg cagttttttt gatgaactga tcgattacat
    27901 gacttccggt cctgtcgttt atcttgtgct ggtcggcgag gaagtgatcg atatcgtccg
    27961 taagatggtt ggagcaacaa aagctgccga tgctgttcca ggtacgatcc gaggagacta
    28021 cgcgctgccg gggactgaaa acatcattca cgcttctgat tcccgtgatg ctgcggtaaa
    28081 agagatcgcc cgctttttcc cgccattcga tataaacaaa ccgtctgaaa aagcggcgaa
    28141 gtgatacaaa ttctaaactg ctggtctagt tgctgggttc agcaatggcc agcagttttt
    28201 tctctaagga cctccgttga ttctttccta acatatattc gagcactgtg aagaattctt
    28261 ggtagtcttt cgtcttttct tcccctaaag cagcagaaat caatctttga taataatctg
    28321 ccatcaatgt ttcatagatc actgtacttc tttctttatg tgctaagctc cgtttcatat
    28381 cttcattcac actacgtgcc tttttcaacc gaccattttc tatataaaac gaagtcaggt
    28441 tcattaaaaa agtaaagaga tactgctcat gattgttcaa ctgtgctaat cgtcgcattc
    28501 tttttttgat tttcaaataa ttgttctcaa tgaaccgatc actgaatatc caaggaaaat
    28561 cagctagaat agatagttca aattgaccat agtctggtat ctgcatcaag tattcttcaa
    28621 tgattcggca ttcataacgt aaaaattcaa attctgtctc agatacttct gttttttcga
    28681 ccatgtgaag attcaaaacc aaataataca gatcttctgt tttcttaaaa tgcctacggt
    28741 tattgctgat ctctttataa ggatcaaagc tgtcttcttg tacccgatct agcattcgtc
    28801 tttttttctt taactttatt ggttcatcca gctggtttcg catccattca tattctgcaa
    28861 gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac aaatgacatt tttttcactg
    28921 aatttgtctc aatcccgact agagaagtct taaaacagat tccttccgct aattgggact
    28981 gtgtcaactt cttgttgact cgaagtttct tgatcaattc accatcatac ataatagttc
    29041 tcctttttaa tttaaatttt tccacattca ataaatagat acgttaaaaa ggaaaaatac
    29101 acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc aaccttcaag cattagaatg
    29161 ttgtctaact actgaaggtt ggtttgattg tttattgatt atttttttgc tgttcaacgt
    29221 ctgttcttgt tactggatat aagttggttg ttgttttatt accatttttt gaaaagacac
    29281 ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt acgttccgct tcatttttat
    29341 agtcatattt tttcggatca atcgtctcaa atccgtttgg cacataaaac cgcaagagat
    29401 tttcctgatt cagcttatct gaaagactta acgcagtctt gactttttcc tgatctttgg
    29461 agatttcctc atcgattcct gctgctttcg tatcgatgat ttctcctgtc tgattattgt
    29521 aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa gttcccattt cgaaaagcaa
    29581 ctacttgatc atgctgagaa gataataagt ctgtaccaaa atgaagataa tctttatcat
    29641 cgattcccaa tagatgtagt aatgtcggca aaacatcgat ttctccaccg tattcatgat
    29701 tgactgtccc tttagtataa ccaggcatat ggatcatcaa tggcactcgt tgcatctgtg
    29761 cgttgtcaaa ttcatcccat gtatctggat ctttaccaag agcgcttgct aaatctttat
    29821 tatctgtatt cgagatccca aaatgatccc cgtaaatcac aaagattgaa ttttggtaaa
    29881 taccagattt tttcaaatac gtaaaaaatt gttccaatga ttgatccaga taatgtgccg
    29941 ttcgaacata attatctact gtgctattgc cagtattcaa agatggaaag tcgaaattct
    30001 tgtcatccgt ataataaggc gtgtggttgg ttacggaaag aaatttcgtg taaaacggct
    30061 gttgtagatg ctcaagatat tgggcagatt ctctgaataa atctttatct aatatcccat
    30121 aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc aaagaaattg tcataaccta
    30181 agtttttata tacatgatct ctattccaaa aactagctac attcccatgg aaaacagcgc
    30241 ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc ttgaaagaca ttgtctgacc
    30301 caagttctgt gaataatgag ccttgaggta aaccaaatgt gccagtttcc aacatatttt
    30361 cagcatcact tgtctttccc tgtcctactt catgaaagaa attgtcaaag ctgatcgttg
    30421 cttgattctg gaaaatacta tttaaaaacg gtgtgacttc ttgtccgtct accttcatat
    30481 taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat cacatttttc cctttagcga
    30541 tcccaaatgt ttcgggattt ggctctgcat aatgtttctt tgtataatct aaaacttcat
    30601 caatcccatt actgcttgca tgcgcgcgaa cactgctagt cattcctgtt ttgattccgt
    30661 cataaatagt atacgcatcg attcctagat atttgacaat atatgaacgg tcaaatgttc
    30721 gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag atttcctgaa aaaataagcc
    30781 ctgaaagaca ggtaacagcg atagccatcg gtttgccgac agggttgctt ttgataggta
    30841 ttttcttgac cttcaaccaa accagtagcc caatgaacac agcgatatcc agccagtaga
    30901 tgacatcatg gggcttcatc aaagaaaagg agcttcctga caatccttgc gacactttcg
    30961 aaaagcctaa tacagatttg actgtaatga aatctgtgaa ttcccgataa aaaataatat
    31021 tcaagtacaa caaaagcgta tttaaaatat caatgatcaa aagaatagct aacgctggct
    31081 ttggcttttt aatatacaaa gaaaggccaa aaaacaataa ggtagtcgca attgggttga
    31141 tccacaagat aaagtactgg atcgtacctt ccacacctaa tgaaaaatct gcatagtagg
    31201 caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa aacgattgct aaacgatttt
    31261 ggcacaattt tttaagttgt gtcacgtata tcacttctct ctattcacat tttttcttat
    31321 tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta tcttaagatt tttttattct
    31381 ttctaaagat tattccgtcc acttttcaca gcataaaacc gctgcgatgt aagcgattaa
    31441 actaatgaag agataaaagt cttgtggtga ccgcttcact ccacggtatt caaaagccag
    31501 tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat tcttgtcgca gctccttatt
    31561 tcttgatgca ggacagcttt ttcacaactt cttccttaaa cggtgttctc ctgccaactc
    31621 ctcggccaca agtcacaata tttataaaac atgagaagct gttttttgaa attgttcctt
    31681 gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc aatcagctga
    31741 ccctcggtat tagattatgt caggacgcta attttacaac ctcttggtaa acggtgttca
    31801 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa atataaggag ctattttttg
    31861 aaattgctcc ttgtggctca gagttaaacg gcaggctcaa gacctcttga tacacggtgt
    31921 tcaatcagct gaccttcggt attagctcaa aaaacggcaa aatatgagga actattttgc
    31981 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctctgt ttttcattgt
    32041 ccataggatt gatattgtcc ttgtgatgac tcattcgtaa taattgaggc ttgattttct
    32101 tcacttcccg ttatcgtttg tccactgcca gaatctgaag gacttgttcc tgtcgactcg
    32161 tttttttgag aagagacagt tgttttcttg acttgttcat ttgtcttttt ccattccgct
    32221 aaacgatcgg cgaccacttt tttgtctact ttttcagaat aagcggtgat cacttttcca
    32281 tttttcaaac gggctacttt gtcgccatca tgagtcaaaa gcaaaacggt caaatgcttg
    32341 tctttaaatt gatattgtct gagagaaatc gcactaagta cgtcatcgat attaataaat
    32401 tgattttctt cttttccttt tggcaaataa atgtccaatt cgcctttttc gtaattggca
    32461 gttattttat actccgccca ccaataagtt gagaattctt ttttgaaatt atctactgat
    32521 tctttgcttg ctacgatttt ttctgtttct ttctgagcaa gatctgctgt catttttcct
    32581 gaaaatgctg cggataaaat aagcagtaag aaaaaaccga ataaccaaaa gagaagaaac
    32641 ggtctttttt tcctcatgct ttcctccact cctatttaat tttgactatt ctattatata
    32701 caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc gtaaataatt gaacttcttt
    32761 ttcacaaaat ggtaagaatg gagaatttca cgcatttgct ctcaactatt ctttcttaag
    32821 cctcaaatcc gtttttatct gatacgtctt tgaaccaatg acccgatttt ttgattgttc
    32881 tttttccatc ttgtgctaag tctactgaaa taaagccata tcgattctta tatgcattgg
    32941 tccacgacca gttatccata catgtccaca tatgatagcc taaacaattg gcaccttctt
    33001 gaatggcttg atggacatat ttcagatgag attggacaaa ttcgatccga taatcatcgt
    33061 ggatcatgcc atcttctttg ataaatcgtg cttcgttttc tacacccata ccgttttcag
    33121 aaataaagca gtcgatattt ccataatgat ctcgtacatt gatcaaaata tcatagattc
    33181 cttgttcata gatctcccat ccgcgataag gattcatttt acgaccaggc atttcataat
    33241 tgtcgaaata atcctcaggc attggaccgt ttttagtatc gattggtgtt tcttttgctt
    33301 ttacccggcg tggctggtaa taattgatac ctaaaagatc tactgtattt tctttgataa
    33361 ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag ttcttttact atctcaatca
    33421 attcacttgg aaactcgcct tttacagctg gatcaaggaa tgaacgattg aagaacgcat
    33481 ctgctatttg cgctgcttta acatcttcag ggtttgattc gtctcttgga tagcttggtg
    33541 tcagattcaa gatgatacca ataacaccac cttgctccat ttcatggtaa gatttgatgg
    33601 ctaacgctga agccaacgct tcatgatagc caacttggat cgcttttttc atatctgact
    33661 cttctggata atgccatcga tataaataac ctgcttcgac tggaacgatt ggttcattat
    33721 gagtaaacca tttttttact ttcgaaccaa acaattcaaa acaacttttg gcatattttt
    33781 cataggcttc aaccgtttct cggttcaacc atccgccttt ttcttgcaga tacatcggca
    33841 tatcgaaatg atacagattc acaaagggtt cgataccgtt ttcaatcaac tcatcaaaat
    33901 aacgatgata gaaacgaacg gcttcttcgt tgattgttcc agattcatca ggtatcaaac
    33961 ggctccattg gatagatgtt cgaaatgaat tatgcccagt ctgtttcatc aactgtacat
    34021 cctcttgata cttcgtataa acttgcgagg ttttttcagg tcctacttga tcaaagaact
    34081 tttgcggttc ttttttgtac caataatcaa aaatgttctc tccttttcca tcgcctgaaa
    34141 tccttccttc tgtttgtggg ccacttgctg cagaacccca ccaaaaattt gctggaaatt
    34201 gatatttttt catcaaagca cctctttttc ttttttagtt cgttttagtt gattccatgt
    34261 atgatatttt ccatcacaat cactttttca ttttcttcct cttctttttt gattcgtttt
    34321 aacagctgtt ctgctgctcg ttccccgatt ttatctgttg gtggcgtgat cgtgattttc
    34381 ggaaggatac tcagatcgtt tttatctaaa taatcatagt gactgaacgc aatatccttc
    34441 ccgattctga caccttcttg attgaaacat tcgatggcac caatcgacat attgtaattg
    34501 ctgacaaaaa tagcagttgc ccctttttct aataatgctt ttgccccgta atagccgctt
    34561 tttctagaat agtctcctgt aaagacctct aattggatcg atggatcatt ttggaccgct
    34621 tctttggctc ctgctaaacg ttctcttgct acgtagtctg tttgagtggc agcaataaat
    34681 ccgatttttt tatgtccttg ttcaccatga tgagcgatca cttttgctac actttctcga
    34741 tcattcacta atattgtatc aatacctgct ggtccttctg gacaattcaa tgcgataact
    34801 ggaatactca tttttgtgag catttcaaat ccagaccact cttcttcgga taagaagaca
    34861 atcagcccat cgatcacatg cttcatcagg tattcaattt tttgttcaat tttttctggc
    34921 tgatcggcaa acccgcttaa taataagcta tacccatttt cttcagctac tctttcgatc
    34981 cctgagacaa cttccgaccc aaaacggctg gaaatcccgt ttatcaaaag tccaatagag
    35041 aagcttcgat tattcttcaa ccctttagca atgatgtttt ctttatagtc caacgctgaa
    35101 atcgcttctg ctattttttt catgttcttt tccttcagtt gctgaccatt caagtaacgg
    35161 gaaactgttc ccactgaaac accagccatc ttagccacat cacggatcga tgccatacat
    35221 tctcactcct gccagttaca tttgatttct ctttcttgtt gttcgattat gatgaccgca
    35281 ttctttgcat agtgttgctt gtcatcaata tccatataac taaagtcttc agtagagcaa
    35341 cttcctctca ctattaatta tacttaaaca agtatttgaa ccggttcatt tttaatatag
    35401 cattttttta aaagattgca acactttttt aaaacgcttt ctgaaagtgg cggatttttt
    35461 gcctttttaa aattttattc taaaatttac tctatttgtg ggtgttttgt gcgtttttct
    35521 cttccatccg tttgaagttt tacattccaa tggtataatt tctttatcct atcaaatggg
    35581 ggaagtattt tgaagaaatt aatcgtacta tttattttgt ctgcgggact tttgacattg
    35641 actggctgtg atttgtttgg cggctctgac caatcctcgg acgaaacaac cgattcttct
    35701 gtcttaaaag cggaaaaagc agataccacc aacagtgtat atacgctaaa agtacgtaaa
    35761 gatatggatt tatccgcggg acagttgaca tttacagata aaaaaatgga atggacacgc
    35821 acgtatcaga cacaaggaac tgattcttct gaatcatctg aagaaaaaac agtgctgaca
    35881 gatatcaaga tcactaccaa agatgacgaa tatattattt caggaaaaga aaataaaaaa
    35941 gaaacaacag tcatatttac aaaaatcggt aattaccgtt tgaaggatga ggatggcaac
    36001 atctacagtc tatagtgccg tctgttggat cttcttttgt ttctcccctt cttttctgct
    36061 atagtaaaga agcaagatga ttgtaaagga gacgaaatgg atgatcgata gtcctgatta
    36121 ttgtacatgt aataacaaca aaaatatcca tacagaattt aatgaccaag gaagcttgag
    36181 catatgcgac aactgtcaca aactagtcaa agattcattt aaaacatttg aatcttatcc
    36241 tgttcaaaat tatgtaaaaa gtagaagaag ataaacaaat ccaagcaaaa ctcactatgg
    36301 ctgattttta tgaacttcaa actcagcttc ttcctttgat caagcttcat cagtaaaaca
    36361 aacagtggat catttcagtt tacaaatcaa agagtaaaga tttatttatt tcaaatattt
    36421 gaatataaaa taaccaaacg atagaaaggc ttattctaca tcacgtagta atttttcttt
    36481 cccgtcgttt ggttttttat ttttagccta attcttgacc ttttgcatgc tggttatccg
    36541 taaaatattt tccagcaggc tttccttcca attcagctaa tttttcttgg atctgacgga
    36601 tcaccttttt tgcatcttct ggattttcta caaaatcgaa tttatcccca tccacttgta
    36661 ttttaggaca tacatcaaaa tcttcatacc attgattata gcgggtgttc aacgtttcat
    36721 aataggtata taacgtctca tcgctttcta gctgttcata gtcacgccca cgttttttga
    36781 tacgttctag catcgtgtcc aaagaaactt tgatatggac catcaaatca ggccgttttt
    36841 ttggagcaac atcatctaac tcgtttagca tcgtatctaa taagctatcg tactgctgta
    36901 cttcgatatc cgttacccga cctaaatcag cgtttagatg gaacaacagg ctatcttcat
    36961 agatggatcg gtctagcaca ttgtcatcgt tcactaaagc atttttcatt gctaaaaatc
    37021 gcttgttcaa aaagaagatt tgcaataaaa aagcgtactg ctcaggattt gaataaaaga
    37081 gcggcaatac ttcgttatct tcgacatttt cataaaatgg tttagaatca atctcttttg
    37141 caagcatatc cgtcaaactg ctcttacctg caccaatcgt tcctgaaatc aaaatcactg
    37201 acatacaacc cttcctcttc tataagtact cgcctacgta agtttcaaca ggcaatgttt
    37261 caaaatcata ttcacgtaca gcttggtctg attttaaata cgtatggaga ctttgtgtaa
    37321 tcatcaagtt cgtaggtacc gtctcttctt gtacaatgat catcggtttt ttcaacatag
    37381 ttgccacacc taattcgtaa gccgttcctg gatcgattgt ttgatgctca aagtcaagga
    37441 tagcaacaac aaattctgca tttgtcagtt cttccatatc tttttcgtat acttcctgcg
    37501 cccatccagc gctgaatagt tcataattgc tttcttggtg atgacgtgga gagtagaaac
    37561 ttgtgactgt tttgttttcc tccaacgctt tttcgatacg acttactcga tcgatttgct
    37621 cctctgaaaa aaatggtcct gctaaatata tttgactcat tattttgcct ccttcaataa
    37681 taactgattt ttaatagata ctgctcgtaa ttcattgacc agttccaatg gattgttgga
    37741 aaagattact tgatccaacc attttttatc cataaaatcc tcttttgcaa aagtttctaa
    37801 taattgttgc aagccgctgt aataaccgcc aatattcaat attccgattg gcttgtcggg
    37861 cgtcagaccg attgctatcc aagaaagaag ttgtgagaat tcctccaatg tacctagacc
    37921 tccagggaaa acgaggacag catctccctt ttcaaataat aatacctttc tttcatcgat
    37981 cgtttctgta ggaataaatg tagtaacatc ttttccaggc aattcagttt caaaaagccc
    38041 attcggatag atacctgtga cttttccgtt attttccagt actgttttac taatcactcc
    38101 cataatcccg gagtatgaac caccgtaaac cagttcgatt tcttcttgtg ccatgtattt
    38161 tcctaacgtt tgcgcaataa atttgtaact ttctttatta ccaaaacggg aaccgcaaaa
    38221 aacagtaagt ttcataaaaa aacactccct aactcaaaag tctatcttca ttctacacgt
    38281 gtttttttaa aataaaagac tagattttgt agttttttag tggtatatag gatagacgcc
    38341 actatatata gtatttagaa aaaaatcatt tttcagccat tttatagata aaacaaaaat
    38401 aaataacgtg ttttcatcga tatgaaatga cttacttata gaagatactg ggatttcgtc
    38461 ctatatgcca ataacgtgta ttattttaaa caacaaatac ttgtttcttt tattaacgaa
    38521 aaaatcattt tgttctcgtg ttatgtttca ctgataaatg atgtttctct aaataaaaaa
    38581 gccgacgaac taaacgatca aaatttgatc atttttgttc atcagcttct cctaaaaatc
    38641 tttttgttaa cgataaatag ctggatttgt aaaaatatca cttgaatcgt cactgctgga
    38701 agaaaattca gtgataaccg cacctgttgc gcctgcctgg aactagtgtt tgatattggg
    38761 ctcgaacgtg tactgatctc tagcatgaag aataatttct ttttttactg tataataact
    38821 ttctttcctt tttggaatag tacaggaaag tttttttgtt tcctttcctt cgacataagg
    38881 tataccagac cctttcgaca acgaaaggtt ctcatttttc cttcatcgtc atttcaagga
    38941 gggtgccgat gctctggaca gtttcgatcg ctcctaaacc aaaatctgta tattcgattc
    39001 tttctaactc tgattctttc ataccattcc tgtctcaagg tacatctatt gtactttttt
    39061 cttgtaatcc attctgcctc tcctaactaa ttgtcaaatc atcccaagat atgtcttctt
    39121 cctctttttc ttcttggatg atttctgcat tcggatctac tggttttttc aataataaaa
    39181 tggttattcc tgtgacaaaa gaaccagcta acaatgcacc aatgaatgcc catggtctgg
    39241 tcatcgtagc gactgcaaac attcccgatg ccggaatacg gctatctgcg ccccaaaaca
    39301 tgcttaatcc gccacctact gtcccaccga ctcccgttgc tatgacattg cgtgccaagt
    39361 cgttcaatac gatagggaaa catccctctg tgatctgaca gatccccata ggaaaagcgg
    39421 tcttcaatgt atcgatttct tgacgattga gtatattttt acgaatgatt ttccctaaga
    39481 aataagcgat cgtcaagccg aatggggtta ccatcgaagc taaaatcaat acagtgattg
    39541 gttcatagat tccttctgaa aatcgtgcca caccccctta tccagaataa atggtaatcc
    39601 agaaacagcc gtgtatctgc ggtaataagc ctaaccaaac gaaaaatatg agaagctatt
    39661 tttgtttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct ctttttttat
    39721 ttatgtttca taatcaatat ggtaaggatg cagctccgta tggtagaccg cctccgaata
    39781 ttcaatcatt tccccaattt gcgtagacga tttccgtatc cgtttgatgc cgatctgtgc
    39841 gtctgttttc agaattcctt gttcttcttc tgttaaaaat actgccgaga aatcatcaga
    39901 aaatgagtca atagtatatc cttttttcgt cagcaaacga tacaaagatt ctttgccaaa
    39961 ttcttctctg ctgaattgca tcatttcttg tggaagataa tgaacaaaat aaatataagg
    40021 ctgattgttc aaataatata agcgcttaaa acaaacagct tgctcaccga aaaaggaaaa
    40081 caaaggatct tccttcttta tatggaccaa ttgcaaatcc agcgtttctt tccggacttc
    40141 atatcgggag ttctccaaga tttgcgtaaa gcttgccgct ttggataatc gattgtaagg
    40201 acgattgctt agaaccgtcg tcccacgacc acttttcttt gcgacatatt catctgatgc
    40261 cagcagttcg attgcctttc ggacagtgat tttgctgact tgaaacagtt cttctaattc
    40321 actttctgtc ggcaacatcg aaccgactgg atattttcct gagaaaatat cttctttaat
    40381 tttatctgcg acttccaagt atagtacacg tgtccttttc ataacgtcac cctttgcttt
    40441 actattacta aaagaatagc acataaaaaa taaaaaaaga acacaaatca catgtgagaa
    40501 gtgtgttctt tttcattcag ctgctttaat tacaagttta cgccatttga ggcaattact
    40561 tctttatacc aatcaaaaga ttttttcttt gatcgattca gtgtcccatt tccttcatcg
    40621 tctagatcca catagataaa gccgtaacgt ttactcattt ctcctgttga tgcactgaca
    40681 agatcgatac atccccaagg cgtatagccc atcaaatcta caccgtctgc tactgcatta
    40741 gccatcgctt cgatatgacg acgtaaataa tcgatccgat aatcatcaat aatcgtgcca
    40801 tcctcttcca tatgatcaat ggctccaaga ccgttttcaa caatgaataa tggtttctgg
    40861 taacgattgt ataattcatt caatgcgatt ctcagccctt ctggatcgat ctgccagccc
    40921 cattcacttg cctcaagaaa cggatttttt actcctccca gcaagttccc ttctgaagtt
    40981 gctgcttgtg gatcagtctc attgattgcc gtagacatat aataactaaa tccgatgtag
    41041 tccactggat attcttttag caactggaag tctttttccg tttgctctaa tttttccgga
    41101 tcgactccat attttttatg ggtacgcgct gtgtatgcag ggtattctcc acgtacttgg
    41161 acatccgtgc agaaaaaatt gaattcttgg ttttggatca ttgttgctgc ctgattgact
    41221 ggattggcat cgatgctgta agttgttgca tagataatca tgcagccgat ttggatatcc
    41281 gaacgcaatt cgtggccgat ttttacggct ttgctacttg caacgaattg attatgccat
    41341 gcttggaaaa tattttggta ctctcctgct ccattgcttt ttactaatcc ttgacttaaa
    41401 gctggaaagt gaaaggccga attgatttca ttgaaagtca tccaatattt gactttactg
    41461 ctgtatctct ctaatactgt ctgagcaaat cgttcataga agtcgatcag ttttcgattt
    41521 ttccagccac cgtattcttt tgccaaatga agaggcattt cgtaatgaga aatcgtgatc
    41581 acaggttcaa tatcatattt taagcactca tcaatcagct gatcataaaa ttctaatccc
    41641 gcttcgttag gagtaccttc gtctccattt ggaaaaattc gtgtccaggc aatcgaaaaa
    41701 cgataacatt tgaatcccat ttccgcaaac aaggcaagat cttctttgaa acgatcgtaa
    41761 tgatcgattc ctcgatgatt cgggtaaata tatttttctt gatcaatcgt ccagtcaaat
    41821 tcttcactgc cgataatcgc aaagcgctgc ttacctccag gcatagcatc tgcaacggat
    41881 agtccttttc cgtctcgatc ataagcccct tctaattgat ttggcagcag ttgctccacc
    41941 ccataaaaat ttttctgaaa aacgtgattg catcctgatt tcctccttat tttacaattg
    42001 ccagaacatc ttcgtttgca ataatctctt tttggtctaa ttcaagtata tcggcaaatg
    42061 catcagtatt cgtaatgaca actggagtga ttacgctata gccagctgct ctaattgctt
    42121 cgatgtcaaa acgaatcaat aaatctcctg ctttgacgag atccccttgt ttgactaaga
    42181 tctcaaagcc attcccattt aattctactg tatccatccc gatatgaatc aaaatctccg
    42241 ctccgtcagt cgttgtgatc cctacagcat gtccagttgg aaaaatagtc gtgatctctc
    42301 cgtctgcagg tgcaactagt tctcctactt ctggatcaat agcgatacct tttcccatca
    42361 cgccagaaga aaaaacttgg tctggtactt cactcaatgg cagtacttta cccgttaaag
    42421 gactttttaa ggtatgccgt gctgaagtaa tcggttttcc tgcattcgca tgtttgtttt
    42481 ctaattgatt gtcttgtgtt tgttcgtcaa atcctaaaat caatgtacct acgaatgcaa
    42541 gaacaaaagc aattcctgta gctattactg ctactgtcac atttgactct acaccatcca
    42601 ccgtattaat gaaagtcgga atcgtcaata agctgaccaa actgctggag aaagctttta
    42661 ctccggaaaa tccgataata gctccaccaa tacctccaga aatacaagcg gcaatgaatg
    42721 gtttcttcaa aggtaatgtc acaccataca cagttggttc agtgattcca aataacgaag
    42781 tcactgtaga agaaatccct aatgctcgaa gtttcgtatc ttttgttcgc aatgctacag
    42841 ctaaagcagc cccaccttgt gccaagatgg ctggaagaag cattggcatc aatacatcaa
    42901 acccatattg ttcgatattc aaaaacatga tcggtacgaa tccccaatgc atgccgaaca
    42961 tcacgaatac ttgccataaa cttcccatga ttagacctgc tacgattgga ctaaatccat
    43021 aaatcgagtt gaagagaccg cctaaagcat taccggcaac cgtcccgatt ggtcctaaag
    43081 caagaaatgt taatggaacc atcactacta aaacgacaag cggaactaaa atcatttgca
    43141 gaaattgcgg aatcactttt tttacgaatg gctctaattt actttgaacc catacggcta
    43201 gaatgattgg tatgacagaa gaagtataac cagatggtga aagaatcact gggatcccaa
    43261 agaaactgat agcttctccc gcaccagcaa ctgctgtgat agctggatac acaagtgcca
    43321 tagcaattgc tactgctaaa aactggttgg ctttgaactt tttagctgct gtaaaagcaa
    43381 gcatgacagg taagaaggta aagacaccat ctgctgcagc aaacaatatt tgataagtac
    43441 cgctatcagc tgtcagccag cccatcaccg ttgccaatga taagaatcct ttcaataccc
    43501 cggcggctgc catcgcccct agaaaaggtg taaatatact agagataata tcgatcagac
    43561 gattcaaaat atttttggac tcttcttttc catcctccga aactgcaaga ccttcctcat
    43621 ccacgatcga ttgataaaca tctgctacat tactgccaat cactacttga tactgaccgc
    43681 cactttgtac gacttggatc acgtccgggt cttcttttaa cttttgcgta tctgctaaag
    43741 attcatcttt taatctgaat cgcaaccgcg tagcacaatg tactaaactt ttgacattct
    43801 tttgtcctcc taccgcttcc catacacgac gaccaactgc ttgattatcc attgtttttt
    43861 ccttctttct tttttattta aaaaataaaa acccagattg aaaaaaacag tcacaaacat
    43921 tcgtgaacct attttttcaa tccaggtttt gcctgccata cagtaacaat ccttatgatt
    43981 ggatgctttt tttctcactt actactcttt gaatatgaat cgttaaataa atctgctctg
    44041 cgatccccat ttcctgtttt tgcgtttgtc gaagatactc attgatccgt tcgacacaat
    44101 gaaacgcttt tggatacttt ccttgaacta atgcgtagag aaattcatct tgctcgtcag
    44161 atccttcccc tttgagataa cgctgggcaa aaaactgcag atgagtaatg atacgctgat
    44221 aattcaaaga ttcttcatcg aacacgatgc cgaagaattt actgatgata ctcagtatat
    44281 ctcttaccat tgtggtagcg gtcatcgtca cttgcatgtc ttcgtttcct tgttggctat
    44341 tcacaaaatg aaaggcaata aaaccggctt catcctcaga aagttccaca cccgtctttt
    44401 gtttcacgaa caaaagagcc tctttcgctg cttgatattc tttcggataa aatttttttg
    44461 tttcaaataa taatggatta ggcagttgga tattttcttt tgctcgtgtg atcgcataat
    44521 gaagatgatc ggtcaatgtc agataaatat taggcgaaaa ttcaactttt agtttatctt
    44581 cagccagttc gactacttgt ttgacccatt cgatctcctc tgccggaata tcagccatca
    44641 gctgctggaa ttggttttta gcaaaagaat ctcttaaaac aaattctttt tggaccgctt
    44701 ctggcgaaat ctcgtcccca atcttttttt gaaaagctag tccttttccc atacaaacga
    44761 cttcttcccc gaatccattt ttcgaaatga cgacattgtt attcaaaatt ttatcaattt
    44821 tcattttgct tcgccctctc aagaaaaata aaaaaaccta gaattcgcct actgtttctt
    44881 agctgaattc tggttttgcc tgccgtacag taacaatcca tttcttattc aaaatcatca
    44941 taccatcgaa tttttattta tgcaagcctt ttcatataaa aaataaaaaa actggaacac
    45001 aagtctatct agaaaacaac caaatcagag atgcacctct ttgcgtttct ctttttttca
    45061 gcaaattggt ttttccttat cgattcatat cctttggctg tttttaatct gtaacgactt
    45121 atgtcccagt atcctaaaat gtttttgtct ctagattgaa agcagatctc atatcatctg
    45181 tcgcatcttt tatcgatgta acactttcca gttctccttg tacaactagt cgcgtgacgg
    45241 cgtccatgtc gccacaagta attagtgtga caatttcttt tccctcgact tcattcagta
    45301 attgggtatc tgtaggagca actttttctt taaaaaatgt tttgtatgta tacacatttt
    45361 ctaaatctgt caaatagatc ttggcaccca tctctaaatt ttctagcggt gtaaacagca
    45421 attcagggtt cgttgcacga tggcttgcta aggcataatt tccttttccc atttcttgat
    45481 ctggagataa tgttcctgct ccgtaaagta gtgacgtatt atccaagcct ttgaaaaccg
    45541 gtaaattgat tgaaactgaa gggactgcga tactagcaat cactggtaga tctgttcctc
    45601 tcatttgcga cctcatgacc ccttctgtcg tcattggttc taccgcatca aagtcaaaac
    45661 tgacatcttt atcgttattc tttttcagat cttcttttgt tacattcgct atagcatatt
    45721 tatctcctgt ctctctgaca aaataatcct tgatctgttc attgaaaatc aaagctagtc
    45781 ctactaataa caagaggaac aagaggatat tgactagcca tctttttgct tttccttttt
    45841 ttttcatttt ctctccctcc agactcattt tactgttttt cttttgctct ttccaacagc
    45901 cgaatgacac atttctacaa ctctgtgtat ttttttcgta cacctgcgct tttttctaca
    45961 ggatatttca aattattttt gatcaatttt tcttcaatga tctcatctag atccaggtga
    46021 aggttgtctg ccatcatgta tgaatagatc aatacatctg ccagttcttc tttgattcgt
    46081 tctaattgtg tttcttttac ttcttccggc gttttccatt gaaaaagttc taacaattca
    46141 gatgcttcca aagaaataga gatagccaaa tctttttcgt tatgaaattg tcgccaatta
    46201 cgttcatctc taaactggtt gactttttcc atactattca tttgcttgcc ttcttccaat
    46261 cacttcttac ttgattaatc ataccatttt tttacataaa aaatagatga tgcttttaaa
    46321 aaaactcctg ttttctttta tttaaaataa atgtcataga aaatcaataa tttaggtagc
    46381 caccatgtgt atgcagtagc gtgtcttctt cccttgaggc tttttgtttt tcgatacgtt
    46441 cttcttccca aacagacaac gctactagaa tctctcttaa tgattttcct tcttcggtga
    46501 gcgagtactc tactttaggc gggacttggt tataggcttt tcgatggata ctttcatctt
    46561 gttctaactc tcttaactgc tgggtcagca ttttttttgt gatcgtaggg atttgcttgc
    46621 ttctccacgc ctaaaataag cgatattgct cttcccttta tctggtgtaa gcaaagcagt
    46681 tagcattttg acgatcgtgg gttccgcttt tcacttctta tctacttagt atcatgaagt
    46741 atactttcgt actaatacta agtagcggaa gtgtcaacta ctttgtttaa ctacatagca
    46801 aaagaaacag aagggtgaca acggtcatca cgcttctgtt tcttttgaat atgctgtgtt
    46861 gttgtctcta tctccgtgta gactttcctc aaacttatcc tttgacagta agtcagtagg
    46921 ctgatttgct ctttctagat gccagataca tttctacaaa aaaatagtga ttctgttttg
    46981 aaaattcgat ttttagttct gcattttcgt aaacatccaa catttttttc gcactgtata
    47041 gacctaaccc gccatttttt ttagaggttt ttcctctttt gaccatctcc gatagcgtca
    47101 aattcgttgg atttgccttg ttcgctatac taaaacgtga tccctctttt tcttccagca
    47161 gattgatcgt aatcggactc tgatcggcta cacgatgatc gaatgcgtta gaaataaaaa
    47221 tagataaaca gcgaataaaa acaaccagat caatcgggat tgttttcact tcgacaggaa
    47281 taactacttg gatcgggatt ttctccgatt ctgctttttc tagaaatttt aaaaagacac
    47341 ccctgacagc tggggataca atatttgtta gctgttgtat ttgcggtttt tcaaaaatag
    47401 ctgcagaatg atcttctaat atttctaaaa attccaacgt ttctttttta ttttcctgaa
    47461 gagtaatttt taatgaaaac agtagacttt tataatcatg tttgaattcc tctagatgat
    47521 ggtaatattc ttcatttatt tgatacagtt gaagcgattc ctgatgttgt ctttctttta
    47581 cttggaaact gatcaacata atggaaaaaa acaaagaaaa aaaactgatg actatcgaca
    47641 aaagagatag aagcagataa ttagatgagc ggaagtgaac acttcccaat accaagtaaa
    47701 tcacgcccca gcttaataaa atacaaatac ctccaaaata gtgttcagtt ggataagtaa
    47761 aaagcaagtt gagaaatctc gtctgtttgt ctatactttt tactaataga tagaaaataa
    47821 atgcgataag tagtgcaaaa aaacttgctg ttccaagatg tctctttcct aaataatcca
    47881 tcagatcaaa aaccagaaaa aagctgagtc cagtgatagc aaaaaaaaga ttactgatca
    47941 aacagctgat aaacaggttt ttcgtagtaa aataaaaaag aaacatcaat aataagccaa
    48001 ctaggtaagg aaagctcact ttagggatag gaagtaaaag tgtcaccacc atcaaagcta
    48061 tacaaccaaa gctgtttttt actagcagtt gtttcgaaaa gaataaatag atacctaaat
    48121 ctaaaatcaa agtaaaggtc aaataaagtg tcactgttgc gaccatctta ttcctccatt
    48181 gttttgtttc aaaatgtaca catatttttt taataggagc ctgcatatga aaaagtattt
    48241 tatcatagaa gataaccagt ttcacagcga atggatcatc agagaattgg tcaatcatca
    48301 tatcgagaat atcgtgcagc tcacttctat ctctcaaatc aatcagttga tccagttggg
    48361 cactattgat aatcacgatg ttttctttat cgatatcgac ttgaagcaat cactcacagg
    48421 tattgatgtc gccgaacaaa tacgtaaaaa aaatgcacat agtaatattg tttttttcac
    48481 cgcttatacg aattttgcag tagagatcat caatcggaaa atccttccga ttggatatat
    48541 cagaaaagac aggaatttat ctgaacaaat tcgtcaagtg ctagaacaga tcaattggaa
    48601 agaacagcag atcgtaaatg tcgataaaat ctttattgat caaaaaggtg gaagtctcgt
    48661 cctgtatcct cgggaaattt tatatattga aagtatcaaa ggtttgaaaa accaagttat
    48721 ggtcaaaaca actacagaag ataaattagt gaatacttct ttaaaacaat tgaagacaga
    48781 gttagaaaag tatgcttact ttttactttt aaaatcttac tgtatcaact tatcgcaatt
    48841 gcagaaaatt gattctgctc attatgtgtt agaatttttc aacggtgact ccttactcgt
    48901 tggtcgaaag atctttgaaa agacaaagga acgtttccat gacttccaaa aaactgcttc
    48961 gagttgatga ataagagctg agacgtaagc atagcagatt tcagggagtg ggccttgaat
    49021 cggctccact ccctgaaatc gttgatatga ttttacgctt cctcttctgt aggagcatct
    49081 gttaacgacc atgtgaatgt tgaaacgtag ttgtctacag cataattgga tacaggaaca
    49141 aataattcca ctgattgtct cccttgttcc acattctctc ctaatgaata aatccatgtg
    49201 ccaatccctt catttgctcc tgcagttact agatctactg cctgatctgc tgggatttcg
    49261 attccttcag cgccagttcc agaaacggta gatggcattc cactgctatt agttgcgcta
    49321 tttcgaatca cggcgttttt gaatttcacc tttgaaccag ttagtttatt cgtatcagat
    49381 gttgtaccaa taaattctgt tcgtttgact gataatttcc aaccggcata atttccgctt
    49441 ttatccgtta cttgtacgaa attctgtact tgtgtttgtg ctccagtact gtccgttccg
    49501 ctttgtaatg cagcatatac tgtttttgct gtacctaact tgattgttcc aaaatcaaga
    49561 tctggagctt ggtcaatcgt cagtaatccg gatgttccct gatttggttc gtcaggtgta
    49621 aaaggagtat caggatcatt tggatcaaca ggaggaactg gaacaacgtc ttcaggattt
    49681 gcagcatctg gtttggtcac agaaatcgtt ccttctgtag tggctgagct cttattctca
    49741 gcaaaaacac tttgtgtaca taataataga cttccagcta gaacggtact taatgcaatt
    49801 gtttttttca tttctttttc cttctttctt ttaaaataaa taatccagca aataagccaa
    49861 taagaatgac tccaacaagt atcagccatc gccagtcaaa tgtttctggc tgatttttta
    49921 aatcaatatc cttttcattc aattggtccg ctgcatcatc agttatttca aaatcggtat
    49981 ggagattcca gcgtttttct ttccaagcac ccttcacaac cgcttgataa tgtcccggag
    50041 ctaatttttt tccttctaat gggacaagta aatcaaaatt tgtattgggt gctacatcta
    50101 ttttttcttt ttttattgaa aggtaaggct cttttgtttc ttttttgtag atttcaaccg
    50161 atgtagtcaa gccgaacata taagaagcat ttggattttg aagattcgcc ttaatcacat
    50221 ttcttccatc gctttgatcc gcataagctt tttttaattt caaatctggg gaagactctt
    50281 tttcgctatt tcaaatcaag attgctttca cttgagaaaa ctgattgacg acaccaactg
    50341 tacttttgtt agaaacgttc tctttttttt cttgaaacgt gatccctcct gcgaccacac
    50401 cagccagctc tttttctggc gctttcagtg aaagtgtctc agtgaccgca ctttgaggag
    50461 gaacagttat ttctcccact gtttttgcta agtttttcat atcataactt aatgtttcat
    50521 caaatttact ttttgattct ccatattcga tcacaccgtt tgaattcgtc gtagcggagt
    50581 ggatcaatgt ctcaactgtt actgatttat ctgttcggtt aaagaccgtg taggggattt
    50641 cctgtgtttt cccaggctcc atccttacat ttatatatgt tttgttctta tcaatctgat
    50701 tagccggtaa ttcaaaatct accgcaaaat tgaattcgga ggcatggaca ggtgtctgcc
    50761 aaaggatgaa tccaaggaga aggatcataa gtaaagagca gttgtttcga tgacttttat
    50821 tccatattcg attcatcaat catcctcctt gatctttatt ctgtcggcgc atctgctaat
    50881 gtccatgtta tctctgctga gtaattacca gctatctgat tttctttggt taagacaagt
    50941 ttaaacgccc gaccgttatt taaatattct gaataatctg cttgcacatt cgttgaattt
    51001 tgtcctctga caatttccag ttcttcttca ttcactaatt gatactcctc tcctttcttg
    51061 aaatatagga catcctgcat agaaaatcct gcatcatttg tgaaaggctg agataatttt
    51121 atcgttaaga tccacggatt tttcgtttct cgctgatcat aaatccataa tttttcctgt
    51181 atatccattt ggccaaatgc ttcttgtgtc aaaccagttg ctttgatctg accaaaatcg
    51241 agtttggaag gggcaagaaa tgagagacca ccttctacta cgatcttcgt agcaggtgca
    51301 aaaggcgggg catcatgata aacaactggc gtatcacttg gattgtgatt cccaatttca
    51361 tcgttggtac ttggcttgtt ctgcacaccc ttagcagtag cttctcctgc ttggtcgttt
    51421 aatacaattt ctaatacatc tcctgcaaaa agttgatttt ctgggataga aagaacaaat
    51481 tcccctgtat catcaatagt tgtttctgac aaaacagctt gatttttttt caatgaaacc
    51541 aaggctcctg cttcacctgt acctgtgatc gtgcgagaag ctgcattgat caatgtggta
    51601 cttaaattag caggttcagg aggaatcaca tcgactgtcg ttttggcaga gtccagttct
    51661 gtcacttctt ggccacctct tactttttta gcagcaacaa cttgcacttg atcctccgtt
    51721 tttaatctct catcattggg tacttgaact tcgaacaagc cgccttgttc gccttctccc
    51781 catgggttat atcctgcccc ttcattttca tctgtttgtc cttgcgtgag agcagtaatt
    51841 tctacagatg atttcgtcgt atctgcgtaa gtaatacgta aagtcacttc tacttcgcct
    51901 gtccaagcat ctcttggctt tgcctcgaca ccttcgggaa caacgacatg cccatagatt
    51961 tttttatctg cattcgttgg cactcttaag gattcaatca tcgggagctg attattggca
    52021 ctgattcgat tataattttt cattccggca cctgtctgag caaattcatt tgttaaacct
    52081 gcactagggc ttgatacgaa attgcctaag tcggtcccat tgatcgataa gtctgtcata
    52141 tcagaaaaat aatccgggtc tttttcaaag ttatcgaatg ttgcgcctgt ttttcgccat
    52201 aaagcgattt ctgaatgggc gatctttaaa gaagaatctc catttccttg gaatacccag
    52261 ccgcctatgc cattggtctc gcttccaatc cgtttgtttt ggaaatcaaa ataggtagga
    52321 gattctaagt ccacattcaa cacatcccca ctgatgatgc cggtcttatc ttcccctgtt
    52381 ttcccgacca tgactaaata agttttttca cctgccgtaa aatttaaatc aatatctcca
    52441 gttgtatcta cagttgctcc ggaatctgcg cgtatatcga tatttgattt ttctccagtg
    52501 actgtaaaat cagctgaacc tggactttct agaaaaccag aagcgtcata aaattggatc
    52561 gcttgatttc cttgattgag tctgttcacg taagatttgc catcacctgt atttcttacc
    52621 ctcaatgagc ctccattggt tactgtcagc ttttgcgtac cattctcaaa acgaactgcg
    52681 ctggaacgcc cactctgctt atcgatgtcg ataactgatc cggctccttc tacgatcaaa
    52741 gaaaggcctt ttcctacaaa acgcatccca ttgttcccgg gttgatcgtc atctccagcc
    52801 attttgaccg agatttgtcc accacttgat acatggattt gactagaatc tccttgcaag
    52861 cgaaatccgc ccgtagttgc tgtattgatg gtgattttcc caccattcga tacagtgact
    52921 ttcgatcctg aacctctcat ttggatgatt ccgcgattgt tgtcattggt aatattgatc
    52981 tttgtattta ccgaaaattc gcttcccgaa ccactgactt caaacatgac attaccaggt
    53041 agcgtatcga ctgcctctcc ttcatcgcta acgccatctg cccgatcata atcaagagag
    53101 acctttgaac cgcttaagat atggattcct ggcgctgttt ttccactacc agtctcatct
    53161 cctgaatcta gaaaaccgta gaatttccct agatacggtc cataaagttg actaccattg
    53221 atcacattca attgatagct tctacaaaaa taggctttac ccggagcggc attcagtcct
    53281 tccttcactg gttggacatc caacacaccg gaaacaccat ctaatgtgac agttgcacga
    53341 ggagcgtaaa tcagtcctaa tttggaccca tcatacaaat caagtgtccc ggtaaatttt
    53401 atttctcccg taaaatgata tctgcttccc caaaaatcgg tcatataagc aatgataatg
    53461 gcatttccat cgatatttgg tgtcaatcca cctggatgtg cagctgttcc tttaaaattc
    53521 tggaactcga tcgactgagg tatctcattg atataaatat ggtgtgtgcc gaaattgtga
    53581 gcgaatccac caaaatcaat aacgatatct cgtttgatct ctaattccct gtctaaagtc
    53641 aaaggctgaa tcagtcggat cttagttact gattcgtctg cgagaagctg cgccagttct
    53701 tctgttgtgg cagcttcgct gtacccatcc gctgaaacag ctcttgccag tgccgcttca
    53761 ctgctatcag acgtttgttc agctacttct gaggtctcac ttgtcttatt ttcttcttgc
    53821 cttttattag tagaagcctc actttgagat tctgtagttt cagaagtaat cgattcttct
    53881 gaaaaagtag tcttgtcaaa actttgattc tctgattgcg ttgattcgtt tgttttctct
    53941 acatcggcaa aaacggatgt caccatcaag agagggaaga caaacaaaat cgacgcaatc
    54001 agctttttta cacattgatt cattctccac catcctctct gacgttttga caattttatt
    54061 ttatcgtttt aatctatttc caattgtact ttcatattta aaatatgtat ttttatggaa
    54121 aaatttttct attaggtgta aatttctttt ttgtacaaaa aaaaggctgt gacaaaagtc
    54181 ttgtcacagt cccttatccc gaacaagcgg tgttctcccg ccaactcctc ggtcacaacc
    54241 tccattttaa caataaacct gtaaatgtga gggcaatact ttgattgtca caggtaattt
    54301 atccccttca tctccatcaa cattcgtcgt caattcttcc tcatcagcta atgacacaac
    54361 taattgctca caagtaaggt aggctagatt tttcgtagat tcatctactc cttttaatag
    54421 gtctggcaat gttttaagcg tatcgatcaa agaagaatct tttaaataaa ccaaatggag
    54481 cttaccatca tccacttttg catttggaag taatgtttca aaaccgccga ctgagttagt
    54541 tagtcctatc agcaaggtac tgctctcaat ttcttccttc tttccatcaa cttctacgtg
    54601 aaacgagtag gattgtgtgg aagctaactg tttgattcct gacatgaaat aagctagttt
    54661 tccaaatttc gtcttcttct ccgcatctac atcattgatt gattcaggga tagagccaat
    54721 tgcgacgaca ttcataaaat agtcgtcatt tatttttcca atatccaatg gcttgactga
    54781 ttcaatcgaa aaatgattga tcgcttcctc aggttctagc ggtatcccta atgctcgagc
    54841 caagtcattc accgtaccca atgggaaaaa tccgaaattt ggccgatgtt cttgttcagc
    54901 gattccactg attccttcgt tgactgttcc atctccgccc atgacaaaca cactatgata
    54961 cccttccgta gccgcttcgc gagtaaagtt ttttgcatcc cctgcttttt ttgtatgcaa
    55021 gacgaccact tcatcaaaca ccgattccaa ttttgctatc gctaattgct caaattcctt
    55081 tgcttgttca ccgcctgaac tggggtttac cactaataaa acttttttca tcactttctt
    55141 tcccttctga ttgatttctt taatataacg atagcataaa gaaaaaaatt aggctgtgat
    55201 gaagtactgt tcatgaaaca ttcatttatt ttttgacaag ataaaacatc catactatga
    55261 aactgaggaa aattaaaaca atgatatagg aaataaaaga aaatgatagg cattgtatac
    55321 ctaataaagt aaaaatctta ggtggcagat aaaaacacaa aaatagcaag agaatgtaat
    55381 agcgtttctt ccactttttg ttatcagacc ttatagcgat cagaggaata ccaaatgcat
    55441 aactgaaaaa ctcttgatgt actgttaata tgtcacggcc ttgaataata aatgcgacaa
    55501 tccccgctat aattagtgtg cctaacacca aaacggaaaa ttgatgaata tattttatat
    55561 acaaatgatc cattgtactt cctgcctttt tatttgtgag aaaaacaaat ccgtatacgt
    55621 gaataacttc tagaaataaa agaaatttgc aaaaccactc catttatcta cttctacgtg
    55681 cattttttga tattgttttc tttatttaag attatacaga aaaatatcta gggtcgctgg
    55741 attctaagta aaaaaaattt ttaaaataaa aagaactaaa tcaagtagta attaaactac
    55801 tgttgattta gttctaataa aaagatttag atgaacagtt ttattgattt aaatagttca
    55861 tattttttat ttataccgct tatttactac ttgctgtact agaatctgaa gtgaatgtac
    55921 tcaaaacatt ttcaaagtct ttgtctttga tttttacatt agcgtctttc aatacttcgc
    55981 caattacttt attctggaag gtactgtcac ttaattttgt gtctgttgca atttctttca
    56041 attctttttt gtatttatcc atgtcgttgc ctttgttttg gtttttcacc attttaacca
    56101 cataatattc cgtagtataa gttgaagcat tggtagaagt gatcacatca gaaacttgac
    56161 catctttcaa cttaaaggct gcttctttga cttctgccgg aaccgttgta gtagtggaat
    56221 caaatttcac ttttccgcca tcttcttttg tcgtagtatc agttgacttg tctttggcta
    56281 atttagagaa atcgtctccc ttatcagcag ctttcttcac atcttttgca tcatcttcac
    56341 ttgctacttg gataatttga gcttccactt ctggatggaa agattcccaa gccgttttta
    56401 aatcttcatc ggtcaatttg atatgtttct ttagaccttc ttgaaaggct agactttgtt
    56461 tgatactgtc tttgaatgtt tgttctgtgt aacctgcaga tttcaattgt gaatcaaatg
    56521 aatcacccaa ttgtttcttt gtttgatcat attgagaatc gatttctttg gttgatacgt
    56581 catctccata tttatcttca aatacttgat aaacgatcaa ctgttgcaca gcggaatctg
    56641 ctccttgcat ttttttcaag ctatccatta cttcttgttc gctgattgta ccgcctttat
    56701 aactaacggc tgttttttca ccggcattcg tacagcctga caataagcca gtgcctagta
    56761 cgatggcaag tgctaaaaat ctttttttca tttatcaatt ctcctacttt tcgttttttg
    56821 cgaattgcta aaaacaaagg tgttcaagat gaaaatcatc aagaacacct ttactaatct
    56881 gctcgatacg tcctgaatag atataaggct acacaatcca gaaagttcgc tcagcagaat
    56941 aaatgtcaag tagcacatca caccttaata tactacttct tttgttaccg tttttcaagt
    57001 ctatcttgcg ttccgtattt aacgtaacta attcttagaa caaatgttat cgattttgtt
    57061 tttcttcttc cgctgctggg atattcaaat cagacacttt tatgttcgat tttccagaca
    57121 tttttttgtt gtttttatta tgatatattt ttatacaact aaaaatgaat atattaaaac
    57181 taccaaaaat aacttttaac gctgtttcat tatatgtttt ttctatttta tataaacttt
    57241 tggatactta ataccaaccg gttggattgt accaacatga gaatgtgtca gttctatttc
    57301 ctgtcgcact tgcataggcc catactcctt ttgttgcatt tgctgatcga atagagtttc
    57361 caactttcgc ttttgcaccg tgacgattat agacacgact atagtggtta gaatgacctg
    57421 tttttctcgt acaagttgta tcactataat aaacccatct tccagagcta tgattaattg
    57481 ctgttctaga aatttcccgg tctactggta aatttgactt aataatatta cctttagcat
    57541 caaaatgaat ctctcctggc tttaaagatt tgtaatagat ttcaccgtga actgaatttg
    57601 cttccacttc agatgacata gcgtttcctc caacaaaacc tatccctagt gcacaaataa
    57661 taactgcttt tttcataaaa cgacatcctt tcctttttga tttttaaagc atgatcttct
    57721 tgtttcctat tttggtcaga tatggatcat gcgtgactac gataactaac gtaccttggg
    57781 aagcttgttg cgataagatt tcaactactt tatccttatt tcttttatcc aatgacccag
    57841 tcggttcatc ggctaaaatg attttgctat tcttcaataa gactctcgca caggcgattc
    57901 tttgcttctc accaccagat aatgtgtaga taggcgtact tagcttcttg tttatattta
    57961 cttttttcaa tgcctcatac tttgctgttc gcttatctga aacatttaaa ttcgaaaact
    58021 tcaatccaat atctaaatta aaatcgattg tttcatattc aatcaaccca aaatcttgaa
    58081 aaagaaaaga tatggtattt ctttttatca agcgcttctc tctcccagtt aattgtttcg
    58141 tctcttgatc gaacaaatag aattgtcctg aactgaactg atcattcaat cctaatatat
    58201 tcaataacgt acttttacct gctccacttt ctccaacaac agtcacaaga tccccagcat
    58261 ccgcagtgaa acgatccaaa tgaagaatcg ttttgtttcg aatgattttt ttcgcacctt
    58321 ttatcactat caacggctcc aattgttaac ctccattcat cagcaataac tgacttttct
    58381 tttctgtaga caaaataaaa acaactgaaa gaataaaatc aatgaagcta aagaaaaaaa
    58441 tattaaaaag atatttaggc tctctttcta taagaaaaag gaagattaca cttgtcgttc
    58501 cccatttgat gaggttcatc atgaagaaat ctttatacgt ttcaaagagc gaataaccac
    58561 ttgaacgaaa gatagcgatt ttccgacaat tgtagacaaa atatatgcat aacatgacat
    58621 agctcaacaa gaagaaagaa aagaaagtga gtaaaaatat ctgtatcaag taatgaaatg
    58681 atctgcttaa cactttgaca gctatctctt cagtctgagt atacggaaat gataactgca
    58741 agttatcacg taactgactt tcttcaatca gctcaaataa cctttctttc gacaaagagc
    58801 ctatcttgat ctttaacggt ggatagattt ctccgctgag aatatttctt tccaaataca
    58861 tactgttttt caatgtcagt atttcaacaa ccggataatg ttcgatccag gggatagaag
    58921 gaataaatgt ataaatgggt tgattgtttt ttataaatag aaatttcggt tctttttcac
    58981 tgatttcttg ataatattct gttatttgcg ccaggtcatt ttttaaatct gtatgtctat
    59041 atcgttcagg gatcaatata acagggtgcg tttctttttc ggttatttgg atcaaccttc
    59101 cagagacatc aatcaaagga aatttcttaa gataattcag atttattttt atccctctgc
    59161 caaactgttc attttcttca ttcaagtaat ctgatacatt gactaatata cttccgtttt
    59221 tattcaaagt ttcatataat tcctgatctg ctgctgatgc aaaacgattg gaataaataa
    59281 aatccacgtg gtttttcccg atggttactg gataaaatac atgatagtcc tcaccagcga
    59341 tttcttttgg tggttcatgg gaaacagcta actgtgaaga agaaaaaacg atagttgcta
    59401 attcgacatt ggcaacggtc aaaagaagag aaaacaacaa tccaaatgta aaaagaatgt
    59461 attggatata gttgaacgct tctcttaacg aagcgatttt ttcaactaat aaaagagaaa
    59521 tcaaagagat accatatatt ccgctgaaca agaaaacgta catctggaac aaataaagaa
    59581 attgctcctt ataatacata aaaataaaac caccaatgat tcctgcaatt atgaatgtaa
    59641 taatgatttt ctttttcccc aataaacacc acaatacttg atgtaatgaa aagtcattat
    59701 tcaacaataa actgatttct ttattcatca ataagaaaaa ggatgataac aaaaggaata
    59761 aaagaatcaa tccaatagtt aaatacactc gataattata tacgtctgta tttgaaataa
    59821 ccgaatacga atagctattt tcaaagtctt caaaaatatt agtggcataa tcagtgtgaa
    59881 atgcttgatt gaatcgatca gtgaatagtg aaatgaaccg ttcataggct tctttttctc
    59941 tcgtcatcaa gaaaaattca ccttcaaaat cttttatttt gatcgattct tcaatggacg
    60001 ttacgcgaaa ggaggtatta gaatagggta ccttctcgtg agaagatttg acatcccctt
    60061 gataatacaa tgtcatttca agctttggct gtatcttgaa gttccaagat gtatctactt
    60121 cattggattc atttactgtt tttttcatat aagaaagctc agtttctgat gagacatttt
    60181 caaataccat gattagctgc tcatattctt cttgcccaat aaaatgttgt ggcaatcgaa
    60241 tcggagtata agtatctgtt tgatggaaaa attcatttgt ccgatagagt acctgtttat
    60301 ttatactaac caaggcaaaa ccactcatga taccggtcac tatgattgtg agtaaaactg
    60361 tgaacttttt tttgattatc accactctcc cttacttaaa tgattctttg ataaaatcat
    60421 accgtttttc atcaaaaaaa cgtatctatc aagtgtatag gtcatgtaga ataaatctat
    60481 atactgtaga atcgaaagga gaagaagatg aacaccggag aaaaaatacg aacttaccgc
    60541 agaaattgta agcttacgca aaaagaatta ggcgaaaaaa ttggtgtttc cgataaaacg
    60601 atttctagtt gggaaaattc tcgtacaatg ccagaccttg aaatgctgtc acttttacat
    60661 catactttag gtctgccgat tgattttcct tctattgaat cagctaccga atcaatagaa
    60721 ccaacattgc cagatgaacc tcttgttaac aagaaatctt attcatcaaa acaacccttc
    60781 tcactcaaaa agacactatc tttctttctg ctcgtctttt gtttggtcta tctttttctt
    60841 atcaattatc aatacaaaaa tgaaacgatc gatcgattca atcctttttt gaaaccacaa
    60901 atcggctata ccacactccc tgtaggatac gtggacaatt ccatcgaacg ttgggtatgt
    60961 aatgatatct ttggccatgg tacatggctg tcttttcacg ctggagaggt agaaaaagag
    61021 aaaaaccatg ctgtaatcaa acacaaagga aaacacgtca tgaacgttcg cctgctcact
    61081 tgggaagcca ttccctcaga ttaccaagaa atcctcagcc aagactatga cgacgcctta
    61141 aaaagaagcc ctcaggagcc agataggcat taagtaggag atgaacgtag agatagaaaa
    61201 aatcaatgca catgatctta tccaaaaaac aggggtacaa attaattgaa aaagccttga
    61261 gatgaaagtt cctaaataaa aaagttcaga atgataggta tttctggctt atagtataat
    61321 atttacgcca cagcctccca atgattcttt ttggaatcat aatcaaggat ggacattcac
    61381 aaaaatattt tgctcattcg atccgaatag attaacgacc aatctagtaa gtaatataga
    61441 ttggtacgta gtcaagctgc acataattta gatcaatttt agttcttttt tatatttcta
    61501 tgatctgctc atttactttt atatgatggc ttacagtaac ataaataaat atgataatcg
    61561 ctggtagttt atccaagagt actcaataaa atacaaaatg gattgttcaa gcgcctatca
    61621 taatgataaa atgaaacaaa taaacgatac aaggaggaag agactttgag taatggcaga
    61681 acaatttttt cattgtaaaa attgttcagt tgttaatcta tgggatttcg gtagcatggg
    61741 cattttttta tagaggaatc acaccaacct ggccgtttta tctcttgtta gtgggaatat
    61801 tagtaggaaa ttttgcccct cttcactatc gtacgaactt ttttccaact gattctggcc
    61861 tttttcttaa aacaaagacg cgaaaattag aaaacattat tgaaatgaca attacctgta
    61921 taatcatttt cttagttata ttcctaaacg tcttctaata agaatctctt aaaattagct
    61981 tgttacagtt agttacctag tttatataac acagttatct tatcttcctt atcaacatct
    62041 tttgccaact ctttgtctta aattcgaaac atataagcag agctacgaac accaagactg
    62101 tttcggctct gttttttgca tcaaattcac tcttatatag ctgataatct cggctcttta
    62161 aaagaaatta ttttcgcttc attttattat aaaaaggcta ggaaccttga tgttatcagg
    62221 ttcctagcct ttatttaagt attaccccac agacccttcc atatagattt ttaaattaca
    62281 cactagttta aactaaaaca aacaaggaaa aacttaccat ttaatcaaaa aattagctca
    62341 tatgaatata ttagaatgta aattagactg gtcaaaatct ggtcaaaaaa gaggacgaat
    62401 acaggtagtc ctcttttttg ttattttgat attctaaatc attttttatt ggattattcc
    62461 cttgttcgat atagacccaa attgatctat caataattat caaactgagt catatagaga
    62521 caaaaatttc aaaaatgacc tgttaaatgg tcatcgaata tcatttaaaa tcaatacgac
    62581 ggagtcagac acggagtcaa aacaagcccg ctgagagtaa aaaatataca gtcccatagg
    62641 taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga ttccatttaa
    62701 tttttatctc attttttcct actagttagt atcaaatact ctcctatttt ctcaataaac
    62761 tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattatagc aaaaaatcac
    62821 tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa aacaaaagaa
    62881 aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac ggtcatataa
    62941 caacggggtg
//
LOCUS       DARBEG010000005        61513 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000005 DARBEG010000000
VERSION     DARBEG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.005"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..561)
                     /locus_tag="SCK20_000321"
     CDS             complement(<1..561)
                     /locus_tag="SCK20_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG1144417.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="SCK20_000322"
     CDS             complement(748..1398)
                     /locus_tag="SCK20_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144418.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="SCK20_000323"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="SCK20_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG1144419.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="SCK20_000324"
     CDS             complement(2910..3908)
                     /locus_tag="SCK20_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG1144420.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="SCK20_000325"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="SCK20_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG1144421.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="SCK20_000326"
     CDS             complement(4924..5655)
                     /locus_tag="SCK20_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG1144422.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="SCK20_000327"
     CDS             complement(5659..6924)
                     /locus_tag="SCK20_000327"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG1144423.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="SCK20_000328"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="SCK20_000328"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG1144424.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="SCK20_000329"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="SCK20_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG1144425.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="SCK20_000330"
     CDS             12266..13768
                     /locus_tag="SCK20_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG1144426.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="SCK20_000331"
     CDS             13770..14507
                     /locus_tag="SCK20_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1144427.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="SCK20_000332"
     CDS             complement(14552..14725)
                     /locus_tag="SCK20_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144428.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15366
                     /locus_tag="SCK20_000333"
     CDS             14701..15366
                     /locus_tag="SCK20_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144429.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="SCK20_000334"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="SCK20_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG1144430.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="SCK20_000335"
     CDS             complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="SCK20_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG1144431.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="SCK20_000336"
     CDS             complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="SCK20_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG1144432.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="SCK20_000337"
     CDS             complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="SCK20_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG1144433.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20579..21745)
                     /locus_tag="SCK20_000338"
     CDS             complement(20579..21745)
                     /locus_tag="SCK20_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG1144434.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21761..21988)
                     /locus_tag="SCK20_000339"
     CDS             complement(21761..21988)
                     /locus_tag="SCK20_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG1144435.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22472..23062
                     /locus_tag="SCK20_000340"
     CDS             22472..23062
                     /locus_tag="SCK20_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG1144436.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23252..23965
                     /locus_tag="SCK20_000341"
     CDS             23252..23965
                     /locus_tag="SCK20_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144437.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24080..25090)
                     /locus_tag="SCK20_000342"
     CDS             complement(24080..25090)
                     /locus_tag="SCK20_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG1144438.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25127..25609)
                     /locus_tag="SCK20_000343"
     CDS             complement(25127..25609)
                     /locus_tag="SCK20_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG1144439.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25759..26127
                     /locus_tag="SCK20_000344"
     CDS             25759..26127
                     /locus_tag="SCK20_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG1144440.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26592..27044
                     /locus_tag="SCK20_000345"
     CDS             26592..27044
                     /locus_tag="SCK20_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144441.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27168..28019)
                     /locus_tag="SCK20_000346"
     CDS             complement(27168..28019)
                     /locus_tag="SCK20_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG1144442.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28032..28817)
                     /locus_tag="SCK20_000347"
     CDS             complement(28032..28817)
                     /locus_tag="SCK20_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1144443.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="SCK20_000348"
     CDS             complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="SCK20_000348"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG1144444.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30114..31037)
                     /gene="truB"
                     /locus_tag="SCK20_000349"
     CDS             complement(30114..31037)
                     /gene="truB"
                     /locus_tag="SCK20_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEG1144445.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31405..33767)
                     /locus_tag="SCK20_000350"
                     /pseudo
     CDS             complement(31405..33767)
                     /locus_tag="SCK20_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34215..34388
                     /locus_tag="SCK20_000351"
     CDS             34215..34388
                     /locus_tag="SCK20_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1144446.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="SCK20_000352"
     CDS             complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="SCK20_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG1144447.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34919..37225)
                     /gene="infB"
                     /locus_tag="SCK20_000353"
     CDS             complement(34919..37225)
                     /gene="infB"
                     /locus_tag="SCK20_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG1144448.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37238..37549)
                     /locus_tag="SCK20_000354"
     CDS             complement(37238..37549)
                     /locus_tag="SCK20_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG1144449.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37546..37839)
                     /locus_tag="SCK20_000355"
     CDS             complement(37546..37839)
                     /locus_tag="SCK20_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG1144450.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="SCK20_000356"
     CDS             complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="SCK20_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG1144451.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="SCK20_000357"
     CDS             complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="SCK20_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG1144452.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39677..44035)
                     /locus_tag="SCK20_000358"
     CDS             complement(39677..44035)
                     /locus_tag="SCK20_000358"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG1144453.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44247..45956)
                     /locus_tag="SCK20_000359"
     CDS             complement(44247..45956)
                     /locus_tag="SCK20_000359"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG1144454.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="SCK20_000360"
     CDS             complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="SCK20_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG1144455.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47453..48253)
                     /locus_tag="SCK20_000361"
     CDS             complement(47453..48253)
                     /locus_tag="SCK20_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG1144456.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48250..49062)
                     /locus_tag="SCK20_000362"
     CDS             complement(48250..49062)
                     /locus_tag="SCK20_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG1144457.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49257..49814)
                     /gene="frr"
                     /locus_tag="SCK20_000363"
     CDS             complement(49257..49814)
                     /gene="frr"
                     /locus_tag="SCK20_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG1144458.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49817..50539)
                     /locus_tag="SCK20_000364"
     CDS             complement(49817..50539)
                     /locus_tag="SCK20_000364"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG1144459.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50675..51556)
                     /locus_tag="SCK20_000365"
     CDS             complement(50675..51556)
                     /locus_tag="SCK20_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG1144460.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="SCK20_000366"
     CDS             complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="SCK20_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG1144461.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52796..53275
                     /locus_tag="SCK20_000367"
     CDS             52796..53275
                     /locus_tag="SCK20_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG1144462.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53492..54583
                     /gene="phnW"
                     /locus_tag="SCK20_000368"
     CDS             53492..54583
                     /gene="phnW"
                     /locus_tag="SCK20_000368"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG1144463.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54576..54704
                     /locus_tag="SCK20_000369"
     CDS             54576..54704
                     /locus_tag="SCK20_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1144464.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54708..55253
                     /locus_tag="SCK20_000370"
     CDS             54708..55253
                     /locus_tag="SCK20_000370"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1144465.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55707..57398)
                     /locus_tag="SCK20_000371"
     CDS             complement(55707..57398)
                     /locus_tag="SCK20_000371"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG1144466.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="SCK20_000372"
     CDS             complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="SCK20_000372"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG1144467.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58880..59899)
                     /gene="argF"
                     /locus_tag="SCK20_000373"
     CDS             complement(58880..59899)
                     /gene="argF"
                     /locus_tag="SCK20_000373"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG1144468.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="SCK20_000374"
     CDS             complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="SCK20_000374"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG1144469.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17113 a        13114 c        10634 g        20652 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc
    16741 ttctctcgaa attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc
    16801 atcaacgaca tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg
    16861 tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg
    16921 tttagttttc atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc
    16981 tgtttctgct tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt
    17041 gtccacagag aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc
    17101 tttatctgct tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt
    17161 tagacctgaa gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc
    17221 agatacattt acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc
    17281 acgaggagct gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc
    17341 cattgggcgt tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt
    17401 tgagaatact tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa
    17461 cactccaccc attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc
    17521 tttcacatca ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc
    17581 agggttaact gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc
    17641 agggatacga gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc
    17701 tgcatctttt agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa
    17761 ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc
    17821 tgtgataaat ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt
    17881 ttcagcagcg tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt
    17941 ttcttttttg aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc
    18001 tagatggttg tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat
    18061 agatacgtca aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt
    18121 tttatctaaa ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc
    18181 tagaccagcg atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc
    18241 tggcacagtg ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt
    18301 gatatattga aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc
    18361 tactttgtaa cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt
    18421 cacagcttga cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac
    18481 agaaggtgtt gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa
    18541 tactgctact gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa
    18601 cgtctccttc tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg
    18661 accatggttg gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca
    18721 atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta
    18781 ggatcaaagg cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta
    18841 cgcaaacttt ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac
    18901 acttctgtgg ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa
    18961 tcttgtgaac ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt
    19021 gcgatttcag cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat
    19081 ttcgctgttt cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg
    19141 acatcgattt ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt
    19201 tctttcacga tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag
    19261 aaagagagaa cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca
    19321 agcgaacggt agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga
    19381 ccgaacatcc gagaataagg catactagta ggccctaaca aggctattgt acctcttcca
    19441 tgaccatcta tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct
    19501 aattcagcac caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt
    19561 ccgtctgagt ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc
    19621 tcataatcca agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca
    19681 agaacggtgt caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc
    19741 atcggaatct ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg
    19801 tcgtttacaa tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg
    19861 aacacttggc tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta
    19921 ttcaagggta cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa
    19981 aaagtcgtat agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg
    20041 atttcatgaa attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg
    20101 cgagcaggtt ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc
    20161 ccagaagaag agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg
    20221 cgaatcgttg ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc
    20281 acgccactac tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt
    20341 aacatcttcc tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat
    20401 atagcaagaa gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca
    20461 agagtgctaa ttctaaataa ttgatgtaaa tccagttata tcaacgtttc aatagtcctt
    20521 tattgtattt ttgaactttt tttatttaaa ttcagataaa aaacaaatat tctttaaact
    20581 atttttcaaa caaaaagcgc tcaaacacat tgttccccaa aaaggtccct cttggtgtca
    20641 aataaacacg gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat
    20701 caccgtacac cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc
    20761 ctgttttttt tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt
    20821 tttcttcttc caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg
    20881 gtcctttgtt gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa
    20941 aatagtggtc attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa
    21001 agttacttat ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa
    21061 acattctggt ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca
    21121 tgaacattgt tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag
    21181 ccaaagcttt ttccaaagta tccagatatc cttctagtgt ttgtcccggt aatgcataaa
    21241 tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa aaattgcata gtttgataca
    21301 catcttctgc ggtgtgttta cgtccaattt ttttcaataa gcgattatca aatgtctgca
    21361 ctcccatgga taaacgattg acgccatagt ttttcattac ttgaagtttt tcacgggtca
    21421 aatcacctgg attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc
    21481 tagcacctga taacagtcga tcaagttgtt tagcagataa agaagttggt gtcccgcccc
    21541 ctacgtaaag tgtttctaaa ttcgctgtcg gatgactttc tagcattatt ttcatttcat
    21601 tcagcagcat ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaaat
    21661 cacagtaata gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt
    21721 ctgcgggatt tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc
    21781 tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt
    21841 tagaatcaag gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg
    21901 cttttacagc ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat
    21961 ctgcaaaaat atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc
    22021 tttgcttaca acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa
    22081 aatccaagaa tcgacttctt ggatgatttt ttctttcatt aaactggtta cctagttata
    22141 tatctctagg gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa
    22201 ttactttttg ccttccagtt tcttttaata ttgtactcaa gcaagtggga attggttctt
    22261 ttgtcatcca tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt
    22321 tttatttagt tcgtatttaa ttaagttgac tatctgtata ctctgtctaa aacttaagaa
    22381 ttgttgtttt cgctgtcgct acccaatttt aatgaatgtc tgtaggttaa aactgttgtc
    22441 cttgttatag atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga
    22501 ggaatatgcc gattgggaag gtgcttatat tactagcaca ctaaaccaaa atgagaaatg
    22561 gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa
    22621 aatcgattac ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg
    22681 caattcatgg gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac
    22741 agacgtacca gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt
    22801 gcaaggttac aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca
    22861 gaataaagaa gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa
    22921 tggaacagct cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga
    22981 agaaataaga gaaaaaacga ttcgtctata caaacttgga ttctatgatt actataaaaa
    23041 gtacggagat cctttctcct aatcattctc ttcaattaaa tactccttat caatagtgtg
    23101 acaagtaatt ttactgatga ttatttgtca cactttttct gacatttatc aaagaagata
    23161 cactctattt atattcatag acaaggtttt acggattcaa aaattcagga ttatgctata
    23221 ctggtattaa aaaaataatg aggtatttta tatgaaaaag ttaggtttta ttttattgtt
    23281 ggtctcttta ttccttgtag gatgtacgtc tcctagtaaa gaaaatagtg atccatctgc
    23341 gaccacagac aaaagttcaa tcagacaaac cagttcagct gaaaaatcaa gtactcagaa
    23401 atcagcttct actgaaacga atacatcttc aacaagcagt aaagtctcag agcccaatac
    23461 ttcttctaca tctagtagta cagttgagac aaatatttct tcttcaacaa tagacacaac
    23521 ctcctcacag tcttctcagt caatagatcc gttagcagga tattctgatc tacaaattga
    23581 gtacgcacgt gtttggctag ctattagagg taatactttt agagatacat cgggtgattt
    23641 cgaattacat gttattcatt atcctgcagg aacacctatc gccccttatg atgcagggag
    23701 tgcagtctat ccgaaagatg tggttatgct cactgggaaa tacagttacc agggagtttt
    23761 agtgtattca agtaaccatg acgggacaat cactagttat ccagttcctt cacattggca
    23821 aatgcctgca gatcaaacat cttatccagc gttcattaga gaaacaactc aagaaatcat
    23881 tgataatgca tctgtagtag ctattcctac aggaaatccc aatgacgtga aacagttaat
    23941 cgatgtgaca gttatagata attagacttt ctaatatgtt atacaaaaag aagctgacag
    24001 cattttgtca gcttctttca ctttcttttc tatttagatt tattggtctt tgatagactc
    24061 caaggcagga aaaaattttt taaataaacg tttgcaaaac agtcgctttt ctatcttcta
    24121 ccgctacttc tgcataggca ccattaatca atgtcagttg tggcggtaca tgaccacaat
    24181 caatatcata caagatcggt agttttaatt cctttgacag atcttcgtat acatccttca
    24241 ttttataatc taacaaatcc tctgccgcac ttctaccgaa catgatcccc gagctgccat
    24301 caaaccagcc tgccagtttc atctgaacaa gtgatcttct taaatcaact gacgatagct
    24361 cacagttttc tagaaaccac aagatcggct ttccatcatt atactttctt ttaaaagatt
    24421 ggacatctcc ataaggagta ccgattaaat gcctgatcgt atcaatacaa ccacctaata
    24481 atcgtccctt catccttact gcatctccgc tgactgattg ccaatttgtt ggttcagtga
    24541 gatggaaaac agaagatgat gaatgctcat ggggccacac tttttgatac ttttcagaag
    24601 attgctgtag aatcgattct ccttctcttg ttttcagtac actttcccac attgctgtcg
    24661 tttcatccaa gtactctcct cttaaatcaa taaggttagt tccatgtgca gtggctatcc
    24721 ctgtttttaa tgtgacagca agtagcaacg tactaatatc tgaataacct actatccatt
    24781 ttggttgtag agcatcaaaa tcgacaaaat cgagaatctc aatcaataat tctcctcccc
    24841 aagtcggaaa gatcatttgg atattttcat cttgcatcat ttgattgaat tcatctgctc
    24901 gttttcttgc tggacctgat ttttccttat cttgcgtcca aagcgtcttt ccagtaacta
    24961 cttggaaaga tttttcttcc attctcttta aagctgattc gagtaacgaa tggtgttcct
    25021 ctgatacgcc tgaggatggt gcagtaaccc caatcgttgc atttctttct aataatggat
    25081 aacttatcat gttgcttcac ctccaagtat gcactatttt acttggttaa ttgttttttt
    25141 cgtttgccgg catcaaaaat atcggcatct caagtagctg gatgattttg tttaagtcaa
    25201 ccgtttggat gaccatcgtt tgatctggtc tacctggtgc aaaaacaagt ttttcatttg
    25261 ataagagctc ttcatcaata ataatcggaa tctgtttttc ataaccaaac ggagaaacag
    25321 cgccctgctt ttgtcccgtt tgttcttcca tgcgttctga tgaaacaata gaaagccttt
    25381 taccgacgat cttccctaat gctttgaaat ccgtttgttt cgtagtaaaa gtcagatata
    25441 cgtagttatt atctttttta tcttttaaga ataagctttt ggtctctgta cctgtaaatc
    25501 cttgttcttt ctttattacc aaagcatctt catttgtata aatggctcga tgatgataca
    25561 actcaaaata tatattttcg tgtaagaatt tctttgccaa gtcaatcatt tttatgtctc
    25621 ttttctattt tattatttga gacatcttat catataaata tagcggttac aacattactt
    25681 gtgttttgtt cgttttttat acataactat tgtttttctg gtaaaataaa tcaaaccact
    25741 taatggaggc gttttagaat ggaaaaattt cagccgatca tcaatcaaat caaagaaaaa
    25801 gaacatcaac aaaagatcaa agaccttttt gctaagataa cagaaacgta tccccagcta
    25861 gacacaactg taaaatggaa ccaacctatg tttactgacc atggtacctt tattattgca
    25921 tttagttttg ctaaaaatca tttttctatc gcaccggaaa aagcagcgat tcgtgccctt
    25981 gaaaaaaata tccaagaagc aggctacgta tatacggata acgtcatcaa agttccttgg
    26041 aaaagtgtaa tcaactggga attgattgaa caattgattt catttaatat tgaagataaa
    26101 aaaggtcatg ataaattttg gcgataaagc tttatacgaa taaacctttt actggcgagg
    26161 aaactattca tcaccttata caggttttgt tcaatcctat cctttttatc atccctggtt
    26221 aaacacttaa ttgacaaagt atctgctatt tttataagga gtctttcttg tgctgaaaaa
    26281 aatcactgat tgaatggctt tgaattttct acatagtaaa attatttttt cagcaatttt
    26341 cctgcaaaac ttttgaataa ggaaaaaata cgagaaaata tcagtgatct ttcataatac
    26401 tgcccattat tttcaggtat atttaaatat aatatcactt taggagagaa cgaatttctt
    26461 tagagaaata aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg
    26521 ttctttctac atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag
    26581 gagggtgaac attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta
    26641 ctacacgttc tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg
    26701 ggcaatggaa attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg
    26761 aagcagcaaa agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc
    26821 ttcaatatcg tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata
    26881 acgatgttta cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc
    26941 atgcggagat actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca
    27001 atgaaaaatt attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct
    27061 agaaattcca gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg
    27121 ttcaaacaaa tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc
    27181 gtgaccatgc ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt
    27241 ggtgtcttat tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc
    27301 tgtgtaatga tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat
    27361 gctgacacac taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat
    27421 gcggttggtt tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc
    27481 ggaaagaaaa tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa
    27541 taaccaatac ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca
    27601 ggatcatcac tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct
    27661 ccaatgattt tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag
    27721 gttccgacta actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg
    27781 aaagatgtat actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc
    27841 aaaaatgtag tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca
    27901 agcaccgctc caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc
    27961 aaaatcagtg gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt
    28021 tttattcacc cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct
    28081 gcaaatagtg taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc
    28141 gccgctattt cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct
    28201 ttttgttcag gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct
    28261 atactattga ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat
    28321 tttaccccgc cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga
    28381 acagaagaca tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc
    28441 gctttcgttc cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca
    28501 aggctaagtt catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc
    28561 agtgtcccaa aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg
    28621 acatctgctt gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt
    28681 tctgctcctt ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat
    28741 cgttgagcaa tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt
    28801 tctaattcct tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata
    28861 acttttattt ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata
    28921 cgctctagca atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt
    28981 tgtgacagaa gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga
    29041 aaaaccattt atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg
    29101 gcttatttat cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc
    29161 ataatgtttt attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt
    29221 tcaatcaatt cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt
    29281 accgcgacat gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc
    29341 agctcccgtc cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat
    29401 gtttccccaa tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc
    29461 tttactttga catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca
    29521 actacccctt ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg
    29581 ctgtctagta atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta
    29641 gaaactgtga cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca
    29701 atgtcttttg gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct
    29761 acgatgtatt gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca
    29821 acttcatata aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta
    29881 gtcaaatatt tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg
    29941 aaagtcatca atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg
    30001 actttctggt gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct
    30061 tctggaattt gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc
    30121 tcatttctca atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa
    30181 atactgacaa cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc
    30241 gcaaatacag tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta
    30301 atatctactc gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt
    30361 gtcccatttt tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata
    30421 ccggcacttg ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct
    30481 gtatctacag ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa
    30541 gaaatggttc ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc
    30601 tgagcctttc taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc
    30661 ttaccgttga cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat
    30721 gaagccatca cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct
    30781 acccgttctc cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca
    30841 tagattttcc cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg
    30901 ggcaacacac cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt
    30961 aagatttttc ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa
    31021 ggtaataacc cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa
    31081 cacattgcat caattactga cgaatttgac atcataatga acggttttta tttttcggca
    31141 ataaaaacgt aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag
    31201 gagggaatag aatggaagat tttttctttg caccatttga ttggatttat gggtggctta
    31261 caggtaaata gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca
    31321 gtcttttttc cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta
    31381 ttttgcggga tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac
    31441 ttctggcaga tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc
    31501 catttttttc gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc
    31561 caaatgataa cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc
    31621 attttctttg tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg
    31681 gaccaatgtt tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg
    31741 atcaaattct tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa
    31801 atctacgaca ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac
    31861 agctgctaat gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc
    31921 ctcctctttt gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc
    31981 tgcttcttct gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat
    32041 tttttctgac gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg
    32101 cagatattca cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc
    32161 ttggtggcta tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag
    32221 cgaaggataa tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg
    32281 agtcaacata gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt
    32341 ccagtcagaa cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg
    32401 atgacgcgac cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat
    32461 tggcgatttg tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca
    32521 aatatacgga aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg
    32581 gtcatatctt ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa
    32641 tctggcataa taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt
    32701 ctttggtcac cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca
    32761 aataattttt ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata
    32821 tgttcttgat ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc
    32881 ccgtcccatt cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat
    32941 tttggcatca cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt
    33001 ttctcgattg cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt
    33061 ccttcaataa agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct
    33121 gatttacgtg ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt
    33181 aaaacagctc catctatcac tggattgata aagacatttg agaaccagct tccagctaaa
    33241 ggaccggttt ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct
    33301 aatacagctc cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa
    33361 cctggtgttt ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat
    33421 aatttttgca ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca
    33481 tctaaataag cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac
    33541 atgttcaaat catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca
    33601 ggaatcgttc cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt
    33661 ctcagtaagg ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc
    33721 caccaagccg tcattttatc aaagtattct ttactagaat aatccataca taacactcct
    33781 tttcattatt actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct
    33841 gttcatttgt ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt
    33901 ttcctttcgc tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt
    33961 tccgcagttc atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa
    34021 cacattgctg gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat
    34081 gtaaaagtct ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt
    34141 tctacaggga ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata
    34201 tcaataactt caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt
    34261 atggtgcagg ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag
    34321 attacaatct agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat
    34381 actactagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg
    34441 acacgccgca agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa
    34501 aatcccgcaa cccctgattt cacaggattt gcgggagttt ttgattagtc tttgttcaag
    34561 tcgcgaagca attcatcgat atggttgcca tagacaactg attcatcaag ttcaaagaac
    34621 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
    34681 gctttattca atccatcttg tgctgttttc ttatctgatg ctaagtcaga taaaatgctg
    34741 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
    34801 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
    34861 tgaccgactc ggcggtcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
    34921 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
    34981 tgaatttttc aatcattgca ccacattcat aacccatttt gacttctttc acgtcatctt
    35041 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
    35101 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
    35161 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
    35221 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
    35281 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
    35341 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
    35401 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
    35461 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
    35521 cttctttcaa ttcgccttct ttcaggcttt caaataagtt atccaaagtc acacggcttg
    35581 tggcagcacg gttttctaat aatgcacgtt tcgcgcgttc ttcacccgct gcacgtgctg
    35641 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
    35701 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
    35761 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaaca tgaagtgttc
    35821 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
    35881 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
    35941 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaga
    36001 tcgtttctcc accccatgct tcaggaatta actcatactc acttaattct tgcataacat
    36061 gttgagggtt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
    36121 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
    36181 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
    36241 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
    36301 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgtaatgtat
    36361 ccaataaagt tgttttccca tggtctacgt gtcccataat cgttacgact ggcggacgtg
    36421 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
    36481 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
    36541 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
    36601 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
    36661 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
    36721 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
    36781 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
    36841 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
    36901 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
    36961 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
    37021 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
    37081 cttgttgcaa tttcttctca tcattgctag taatcgcacc catatggttt ttcacatcga
    37141 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
    37201 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
    37261 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
    37321 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
    37381 cttttatctt ttttcttgta ttctcgcttg catcggttgc cacaaacacc aattttgctt
    37441 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
    37501 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
    37561 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
    37621 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
    37681 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
    37741 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
    37801 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
    37861 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
    37921 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
    37981 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
    38041 agtcaattta gcagctaaac gtgcgttttg tccgcgttta ccgattgcta gcgataattg
    38101 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
    38161 gacttgtgca ggattcaatg catttgcaat gaatacagct ggatcttcat tccattcgac
    38221 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
    38281 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
    38341 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
    38401 tacttcttgc tcaaataagc ggcgcaatag gtctggatgg ctgcggctaa caaatacttg
    38461 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
    38521 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
    38581 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
    38641 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
    38701 ttgtttagcc gtttgcgcag cgatccgccc aaaatctttt ggtgttactt caaaacggat
    38761 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
    38821 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
    38881 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
    38941 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
    39001 taaagcgtct aacgcattta acatttcttt actcattttt tctgattcct ctccgtgatt
    39061 aaaattggat agctaagcga gcttttgcaa tattttttcg gtcaaaagtc aattcttttt
    39121 ctcgtgtttt tatgcgtatt ttcaatgtaa gctgttcagc atcaaatgat tgaagaaatc
    39181 cttcatattg tttttcgcca tctactggtt ggtataagga gacatggaca taatcaccaa
    39241 gtgccttttc atagtcgctt tcttttttca atggccgctc tgctcccggg gaggatacct
    39301 ccaggaaata agcttgagga atcggatctg gatctgctgc atcaagtttt tcactcagtt
    39361 tctcactgac aaaggcgcat tcttcgatat cgataccgcc ttctttgtca ataaatactc
    39421 gtaagaacca gctttttcct tcttttacaa attcgacttc gacaagttca aaattttgtt
    39481 catctaaaat cggtgtcacc atttctgtga ctgtttcaac gacgctactc aaaagtttcg
    39541 cctccttaaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
    39601 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
    39661 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
    39721 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
    39781 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgttttg
    39841 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
    39901 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
    39961 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
    40021 ctaatccttt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
    40081 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
    40141 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
    40201 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
    40261 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
    40321 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
    40381 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
    40441 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
    40501 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctag catccctcga acaaaacgag
    40561 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
    40621 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
    40681 gtggatcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
    40741 gaatatctaa tttcaataca ttatcgtgaa tcgagtgaaa atcaaaatga gtcgttttcc
    40801 attccgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
    40861 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
    40921 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
    40981 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
    41041 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
    41101 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
    41161 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
    41221 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
    41281 gacaataata atgaggggct aacggattaa cttcagtgat tcctgtcatc gtcgcaacaa
    41341 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
    41401 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
    41461 ctttttttaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
    41521 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
    41581 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
    41641 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
    41701 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
    41761 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
    41821 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
    41881 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
    41941 taacttcaat ataatcgtag aattttgctc ggtttcttgc ctcttctacc cctttttgca
    42001 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
    42061 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
    42121 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
    42181 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
    42241 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
    42301 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctgacacca aattttttag
    42361 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
    42421 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
    42481 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
    42541 acatccgcaa tacttcttct tccgactttg atcctcgaac cattccatcc gtgataccag
    42601 tcagatctac cgttgttcgt gacaaaggat gtcctggatc gataaattca tcaaagcttt
    42661 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
    42721 cagacaatcc tgtcgtttcc acgtcaaata cgacatatgt ggcttcactc aatgcttcat
    42781 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
    42841 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
    42901 cgtggtctgt aatggcgata gctcggtgtc cccatttccc tgcttgtgcc acaagatcag
    42961 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
    43021 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
    43081 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
    43141 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
    43201 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
    43261 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
    43321 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
    43381 tgtggatggg tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt
    43441 ggacaactag actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt
    43501 cttcaaaaag tttcaatact cgttccgctt gctgctcatc cacttctggt tcgatacgaa
    43561 attttgggaa tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca
    43621 aataagaaat agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag
    43681 gtacctgcgt tttcaatact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag
    43741 cttgggccca gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat
    43801 tcagtttgac ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat
    43861 atagcataat aggcaaaatt ttggaaaaat gcagcgtaaa ttcccataaa cgcgatttac
    43921 gatggaccac tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt
    43981 cctctagttg gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata
    44041 tacgcccttt ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt
    44101 accttttagt atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa
    44161 atcgcagttc caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat
    44221 ttttattttc tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta
    44281 ttcaccaatt cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct
    44341 acgattccat ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca
    44401 gaatccgcga atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca
    44461 gcttttgtca tcgcttcttc tagttcattc gtcaggtttg tttggtattc gtcttttaga
    44521 ttcatttgga cgatatgcaa atcaaatggc gcaattcctt ttggccagtt gattccttgt
    44581 tcatctgaat gttgttctac gattgctgat aataaacgac ttacaccgat accataacag
    44641 cccataatca caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca
    44701 ctgtaacgag tgccgatttt gaagatatgt ccgatttcga ttcctcgagt aaaggcaaga
    44761 acaccattgt tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa
    44821 agtacagtga aatcgcgttc tggattcaca tttttatagt ggaatcctgt ttcgttcgct
    44881 ccagtaatgg cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct
    44941 gaaacaccga ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct
    45001 tctgttgctt cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact
    45061 tcatggtctc cgcgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac
    45121 ttgatgattt tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg
    45181 ttaggtgtag ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag
    45241 tagcttgtag ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta
    45301 tcctccccga tttctgagat cgccatgaat tcttttgagt ctttcccgcc cattgcgcca
    45361 ccgtctccga taatcgagcg aaattctaag ccacaacgct tgaatacttc ggtgtaggct
    45421 ttttcgtaat ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatacgca
    45481 tctttcatga taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga
    45541 tatttaggtt ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta
    45601 cgcatcaaat cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg
    45661 cggtctttga aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa
    45721 agttctgctg gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct
    45781 tcccgcataa tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa
    45841 atacccgctg atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact
    45901 tctgcatcat ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta
    45961 tatactcctt tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt
    46021 tttctagaaa aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa
    46081 aaatccaaag ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg
    46141 aatcccttca aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag
    46201 attgacgatt ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc
    46261 ttgatttgct gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa
    46321 actgaaacct gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc
    46381 ttgacgcgtc cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc
    46441 aagttggcca actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg
    46501 cacctgacct tctcgttcga tcttaaagtc taatgtttta tccggatttt tcgtaatgac
    46561 tgttgtcaga tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt
    46621 ttcttttaag cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt
    46681 gttcgtcact tgaacaccac cttgcataaa tgccagaatg atgaataaca ggattgccaa
    46741 aataaagtta ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga
    46801 ttggaactgt acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt
    46861 cgcatcgtga tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt
    46921 gatagtcagc tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa
    46981 ttggattttc ttacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga
    47041 aagaggcatt cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata
    47101 accgccaata ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata
    47161 aattttcgga cccataccga tagcaaattc gcgaacgagg attcctgatc gtttcgcaaa
    47221 gaagaaatga ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag
    47281 aatcgttttc atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga
    47341 taaaacacta taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa
    47401 aataaaaacc tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa
    47461 tgccaaataa atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc
    47521 caccatggcc tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag
    47581 attcgaccaa atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga
    47641 caaacatacc atgattaaat agttcttttg ctgggtatag caataaataa agaaatgcga
    47701 ctacaacagc actaccaata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat
    47761 caggacttaa cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg
    47821 cccaaacaat aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa
    47881 aattctggaa acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa
    47941 tcgtatacgt atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa
    48001 ataacataaa tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa
    48061 caagtaacac tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt
    48121 tcatccgaaa cagctcgtaa actcctatta ctgctaagat tgctgctgta agctcaaccg
    48181 ctatgcctcc gatccatata atcggaataa acaagactag cgccacggct gctgtgacca
    48241 cacgttgttt catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg
    48301 acggtctcga ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg
    48361 ccaaagcgct tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact
    48421 aattcgctct tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa
    48481 gaaacctgtc attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc
    48541 tccttttgct acttcttcgg caatttcttg gacagcagtc acgatctctg ctctgcttcc
    48601 ataattcaaa gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg
    48661 tcttacagca tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt
    48721 gacattttct ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat
    48781 gagaaaactt acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt
    48841 caacactttc actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc
    48901 ttctttatgg ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc
    48961 catgatgatg gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt
    49021 ttcttgaata tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact
    49081 taaaagattc ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc
    49141 attgtttttt tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa
    49201 atataaataa aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa
    49261 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
    49321 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
    49381 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
    49441 tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc
    49501 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
    49561 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
    49621 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
    49681 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
    49741 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
    49801 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
    49861 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
    49921 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
    49981 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
    50041 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
    50101 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
    50161 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
    50221 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
    50281 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
    50341 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
    50401 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
    50461 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
    50521 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
    50581 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
    50641 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
    50701 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
    50761 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
    50821 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
    50881 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
    50941 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
    51001 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
    51061 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
    51121 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
    51181 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
    51241 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
    51301 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
    51361 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
    51421 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
    51481 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
    51541 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
    51601 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
    51661 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
    51721 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
    51781 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
    51841 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
    51901 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
    51961 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
    52021 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
    52081 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
    52141 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
    52201 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
    52261 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
    52321 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
    52381 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
    52441 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
    52501 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
    52561 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
    52621 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
    52681 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
    52741 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
    52801 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
    52861 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
    52921 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
    52981 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
    53041 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
    53101 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
    53161 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
    53221 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
    53281 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
    53341 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
    53401 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
    53461 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
    53521 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
    53581 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
    53641 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
    53701 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
    53761 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
    53821 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
    53881 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
    53941 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
    54001 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
    54061 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
    54121 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
    54181 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
    54241 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
    54301 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
    54361 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
    54421 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
    54481 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
    54541 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
    54601 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
    54661 ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
    54721 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
    54781 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
    54841 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
    54901 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
    54961 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
    55021 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
    55081 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
    55141 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
    55201 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
    55261 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
    55321 aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg
    55381 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
    55441 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
    55501 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
    55561 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
    55621 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
    55681 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
    55741 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
    55801 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
    55861 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
    55921 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
    55981 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
    56041 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
    56101 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
    56161 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
    56221 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
    56281 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
    56341 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
    56401 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
    56461 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
    56521 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
    56581 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
    56641 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
    56701 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
    56761 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
    56821 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
    56881 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
    56941 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
    57001 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
    57061 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
    57121 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
    57181 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
    57241 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
    57301 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
    57361 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
    57421 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
    57481 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
    57541 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
    57601 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
    57661 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
    57721 agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
    57781 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
    57841 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
    57901 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
    57961 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
    58021 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
    58081 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
    58141 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
    58201 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
    58261 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
    58321 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
    58381 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
    58441 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
    58501 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
    58561 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
    58621 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
    58681 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
    58741 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
    58801 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
    58861 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
    58921 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
    58981 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
    59041 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    59101 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    59161 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    59221 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    59281 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    59341 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    59401 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    59461 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    59521 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    59581 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    59641 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    59701 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    59761 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    59821 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    59881 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    59941 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    60001 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    60061 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    60121 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    60181 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    60241 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    60301 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    60361 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    60421 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    60481 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    60541 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    60601 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    60661 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    60721 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    60781 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    60841 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    60901 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    60961 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    61021 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    61081 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    61141 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    61201 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    61261 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    61321 aatattcgat aattaaataa ttaaacgttt ttctattttt aagcgtaatt tattcatttt
    61381 ttattctgta tacactttca aaaaaatatt tattcattga aaaaattttt ttgaataaat
    61441 taaaaaaata aattatatta tcgttgttag cgtttactat atgtattatt gttgtttttt
    61501 atacacaaat cat
//
LOCUS       DARBEG010000006        60875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000006 DARBEG010000000
VERSION     DARBEG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.006"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            199..2733
                     /gene="secA"
                     /locus_tag="SCK20_000375"
     CDS             199..2733
                     /gene="secA"
                     /locus_tag="SCK20_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG1144470.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            2859..3960
                     /gene="prfB"
                     /locus_tag="SCK20_000376"
     CDS             join(2859..2930,2932..3960)
                     /gene="prfB"
                     /locus_tag="SCK20_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG1144471.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4065..4751
                     /gene="ftsE"
                     /locus_tag="SCK20_000377"
     CDS             4065..4751
                     /gene="ftsE"
                     /locus_tag="SCK20_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG1144472.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4735..5628
                     /locus_tag="SCK20_000378"
     CDS             4735..5628
                     /locus_tag="SCK20_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144473.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            5780..7228
                     /gene="cls"
                     /locus_tag="SCK20_000379"
     CDS             5780..7228
                     /gene="cls"
                     /locus_tag="SCK20_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1144474.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            7565..8422
                     /locus_tag="SCK20_000380"
     CDS             7565..8422
                     /locus_tag="SCK20_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG1144475.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8537..9457
                     /gene="pstC"
                     /locus_tag="SCK20_000381"
     CDS             8537..9457
                     /gene="pstC"
                     /locus_tag="SCK20_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG1144476.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9457..10341
                     /gene="pstA"
                     /locus_tag="SCK20_000382"
     CDS             9457..10341
                     /gene="pstA"
                     /locus_tag="SCK20_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG1144477.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10356..11162
                     /gene="pstB"
                     /locus_tag="SCK20_000383"
     CDS             10356..11162
                     /gene="pstB"
                     /locus_tag="SCK20_000383"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1144478.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11178..11936
                     /locus_tag="SCK20_000384"
     CDS             11178..11936
                     /locus_tag="SCK20_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1144479.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11949..12626
                     /gene="phoU"
                     /locus_tag="SCK20_000385"
     CDS             11949..12626
                     /gene="phoU"
                     /locus_tag="SCK20_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG1144480.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            12864..13895
                     /locus_tag="SCK20_000386"
     CDS             12864..13895
                     /locus_tag="SCK20_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1144481.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            13892..14659
                     /locus_tag="SCK20_000387"
     CDS             13892..14659
                     /locus_tag="SCK20_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1144482.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            14759..16327
                     /gene="liaX"
                     /locus_tag="SCK20_000388"
     CDS             14759..16327
                     /gene="liaX"
                     /locus_tag="SCK20_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG1144483.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16624..16944
                     /locus_tag="SCK20_000389"
     CDS             16624..16944
                     /locus_tag="SCK20_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG1144484.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            16944..17294
                     /locus_tag="SCK20_000390"
     CDS             16944..17294
                     /locus_tag="SCK20_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG1144485.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            17461..18399
                     /locus_tag="SCK20_000391"
     CDS             17461..18399
                     /locus_tag="SCK20_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG1144486.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18414..19244
                     /locus_tag="SCK20_000392"
     CDS             18414..19244
                     /locus_tag="SCK20_000392"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG1144487.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            19288..20313
                     /locus_tag="SCK20_000393"
     CDS             19288..20313
                     /locus_tag="SCK20_000393"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1144488.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20328..21266
                     /gene="galU"
                     /locus_tag="SCK20_000394"
     CDS             20328..21266
                     /gene="galU"
                     /locus_tag="SCK20_000394"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1144489.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            21391..21831
                     /locus_tag="SCK20_000395"
     CDS             21391..21831
                     /locus_tag="SCK20_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG1144490.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            21834..22436
                     /locus_tag="SCK20_000396"
     CDS             21834..22436
                     /locus_tag="SCK20_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1144491.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(22495..23601)
                     /locus_tag="SCK20_000397"
     CDS             complement(22495..23601)
                     /locus_tag="SCK20_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1144492.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            23811..24812
                     /gene="ccpA"
                     /locus_tag="SCK20_000398"
     CDS             23811..24812
                     /gene="ccpA"
                     /locus_tag="SCK20_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG1144493.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(25149..27494)
                     /locus_tag="SCK20_000399"
     CDS             complement(25149..27494)
                     /locus_tag="SCK20_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1144494.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            27773..28675
                     /locus_tag="SCK20_000400"
     CDS             27773..28675
                     /locus_tag="SCK20_000400"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG1144495.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            28888..30663
                     /locus_tag="SCK20_000401"
     CDS             28888..30663
                     /locus_tag="SCK20_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG1144496.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(30729..32315)
                     /locus_tag="SCK20_000402"
     CDS             complement(30729..32315)
                     /locus_tag="SCK20_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG1144497.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            32566..32790
                     /locus_tag="SCK20_000403"
     CDS             32566..32790
                     /locus_tag="SCK20_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG1144498.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            32932..34683
                     /locus_tag="SCK20_000404"
     CDS             32932..34683
                     /locus_tag="SCK20_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1144499.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            34683..36467
                     /locus_tag="SCK20_000405"
     CDS             34683..36467
                     /locus_tag="SCK20_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144500.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            36793..37644
                     /locus_tag="SCK20_000406"
     CDS             36793..37644
                     /locus_tag="SCK20_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1144501.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            37698..39449
                     /locus_tag="SCK20_000407"
     CDS             37698..39449
                     /locus_tag="SCK20_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1144502.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            39453..39623
                     /locus_tag="SCK20_000408"
     CDS             39453..39623
                     /locus_tag="SCK20_000408"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1144503.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(39663..40565)
                     /locus_tag="SCK20_000409"
     CDS             complement(39663..40565)
                     /locus_tag="SCK20_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1144504.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            40796..42199
                     /locus_tag="SCK20_000410"
     CDS             40796..42199
                     /locus_tag="SCK20_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1144505.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            42199..43632
                     /locus_tag="SCK20_000411"
     CDS             42199..43632
                     /locus_tag="SCK20_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1144506.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            43777..44703
                     /gene="trxB"
                     /locus_tag="SCK20_000412"
     CDS             43777..44703
                     /gene="trxB"
                     /locus_tag="SCK20_000412"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG1144507.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            44917..46644
                     /locus_tag="SCK20_000413"
     CDS             44917..46644
                     /locus_tag="SCK20_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG1144508.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            46659..47009
                     /locus_tag="SCK20_000414"
     CDS             46659..47009
                     /locus_tag="SCK20_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1144509.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(47101..47961)
                     /locus_tag="SCK20_000415"
     CDS             complement(47101..47961)
                     /locus_tag="SCK20_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG1144510.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            48053..48481
                     /locus_tag="SCK20_000416"
     CDS             48053..48481
                     /locus_tag="SCK20_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1144511.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            48643..48819
                     /locus_tag="SCK20_000417"
     CDS             48643..48819
                     /locus_tag="SCK20_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG1144512.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            48846..49292
                     /locus_tag="SCK20_000418"
     CDS             48846..49292
                     /locus_tag="SCK20_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG1144513.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="SCK20_000419"
     CDS             complement(49446..50825)
                     /gene="brnQ"
                     /locus_tag="SCK20_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1144514.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            51531..52502
                     /locus_tag="SCK20_000420"
     CDS             51531..52502
                     /locus_tag="SCK20_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG1144515.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            52526..54718
                     /locus_tag="SCK20_000421"
     CDS             52526..54718
                     /locus_tag="SCK20_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG1144516.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            54735..55211
                     /gene="ybeY"
                     /locus_tag="SCK20_000422"
     CDS             54735..55211
                     /gene="ybeY"
                     /locus_tag="SCK20_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG1144517.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            55195..55590
                     /locus_tag="SCK20_000423"
     CDS             55195..55590
                     /locus_tag="SCK20_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG1144518.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            55605..56504
                     /gene="era"
                     /locus_tag="SCK20_000424"
     CDS             55605..56504
                     /gene="era"
                     /locus_tag="SCK20_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG1144519.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            56647..57459
                     /gene="recO"
                     /locus_tag="SCK20_000425"
     CDS             56647..57459
                     /gene="recO"
                     /locus_tag="SCK20_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG1144520.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            57843..58760
                     /gene="glyQ"
                     /locus_tag="SCK20_000426"
     CDS             57843..58760
                     /gene="glyQ"
                     /locus_tag="SCK20_000426"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG1144521.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            58762..60837
                     /locus_tag="SCK20_000427"
     CDS             58762..60837
                     /locus_tag="SCK20_000427"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG1144522.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        19863 a        10760 c        13027 g        17225 t
ORIGIN      
        1 aaaacttgca gcagctactg acagctgccg caagtttttt tgagtttcct cctcttttag
       61 atgaaatatg aataaattat gaagttctta ctttaatacg cttaagatat ttcaattatc
      121 tataactaat gataaaatga aaagtaaagt ctacatagtc tggataatac acagaaggaa
      181 aaggagatta cacaacgcat ggccaatttg attcgttcac ttatcgaaaa cgataagaaa
      241 gaactaaaac gtttagatag cattgcaaaa aaagttgaag catacgctga ccaaatggca
      301 gcattaactg atgaacaatt acaggcaaaa actgctgaat tcaagggcag ataccaaaaa
      361 ggagaaacat tagatcaatt acttccggaa gcttttgcag tcgtccgaga agcagcaaaa
      421 cgtgtattgg ggttatatcc acaccacgta caattgatgg gtggaatcgt gcttcacgat
      481 ggaaatatcc ctgaaatgcg tactggtgaa ggtaaaacct tgaccgcaac gatgccagta
      541 tatctaaatg ctctgactgg cgaaggggta cacgtagtta ccgtcaatga atatttagca
      601 acccgtgact cgacggaaat gggcgaactt tataatttct taggattaac agtggggttg
      661 aatatcaact caaaatcttc ggatgaaaaa cgcgaggctt ataattgtga tatcacttat
      721 agtacgaata atgaattagg atttgactac ttgagagaca atatggtcgt ttaccgccat
      781 caaatggtac aacgtccgct gaattatgcc attgtcgatg aagttgactc aatcttgatt
      841 gatgaagcga gaacgccgct gatcatttca gggcaagcag aaaaatccac agctttatat
      901 acaagagcag acaacttcgt gaaacgtttg aaagaagaag aagattataa aatcgatgta
      961 caatcaaaaa caatcagttt aaccgaagca gggatcgaaa aagcagaaga aacattcggt
     1021 ttggataact tatacgacat tgaaaatact gcattgaccc atcacttgga tcaagcattg
     1081 agagccaact atatcatgct tttagatatc gattatgttg tccaagacgg aaaagtactg
     1141 attgttgacc agttcactgg tcggatcatg gatggacgcc gttattcaga tggacttcac
     1201 caagcaatcg aagctaaaga aggcgtagag atcgaagacg aaacgaaaac catggcgacg
     1261 atcactttcc agaactattt ccgtatgtac aagaaacttg cgggaatgac tggtacagct
     1321 aaaacagaag aagaagaatt cagagaaatt tataacatcc aagtatacca gattccgaca
     1381 aatcgtccgg ttatccgtga tgatcgtcca gatttgcttt acccaacatt acaaagcaag
     1441 ttccacgctg tcgtgcaaga tatcaaagat cgctaccgca aaggtcaacc tgtgttagtc
     1501 ggaactgttg cggttgaaac atctgagtta ttatcagata tgctgaatca agaacgtatt
     1561 cctcatgaag tgctgaatgc gaaaaatcac tttaaagaag cagagatcat catgaatgct
     1621 ggtcaaaaag gtgctgtgac gattgctacc aacatggctg gtcggggaac agatatcaag
     1681 ttaggacttg gggtaagaga acttggtggt ctagcagtta tcgggacaga acgacatgaa
     1741 tctcgtcgta tcgataacca gcttcgagga cgtgcaggac gtcaaggaga ccctggtatg
     1801 tcacagttct acttgtcatt ggaagatgat ttgatgaaac gattcggttc agaacgaatc
     1861 aaatctttct tagatcggat gaagatcggt gaggaagatg ctgttatcca aagcaagatg
     1921 ctgacgaaac aagtagaatc tgcacaaaaa cgtgtagaag gaaataacta tgacacacgt
     1981 aaaaacgtct tacaatacga tgatgtgatg cgtgaacaac gtgaagtaat ctataaacag
     2041 cgacaagaag taatcatgga agaaaaaagc ttatccaagc cactaatgaa catggtcaaa
     2101 cggacaatca gtcgtgtagt agatgcgcat acacaattag agaaagaaaa ttggaatttg
     2161 gaaagtatca tcgattttgc cggcagtact cttgtccatg aagatagtat tactcttgca
     2221 gatatcgaag gaaaaacagc tcaggagatc aaagagtatc taaacgaacg agcacaagaa
     2281 gtatttaata ccaaagcggc tcaactaaat ggtccagagc aactgttgga atttgaaaaa
     2341 gtcgtgatct tgcgtgttgt ggactctaaa tggacagacc atattgatgc aatggatcaa
     2401 ttacgtcagt caatcggctt acgtgcttat ggacagaaca atccattagt cgaatatcaa
     2461 acagaaggct ataagatgtt tgaagatatg gtaggcgcca tcgagtatga agtcactcga
     2521 atctttatga aagctgagat ccgtcaaaac gtacagcgtg aacaggtttc tcaaggaaca
     2581 gcttctcact cagaagacgg agatgccaaa gaaaatagta atactagcgc gaaaaaacag
     2641 ccaatccatg tgaacaaggt cggaagaaat gatccttgtc cttgtggaag cgggaaaaaa
     2701 tataagaact gtcatggaaa aaatgcaaac taatttctga cagcaggatt ggtgagacaa
     2761 attctttttt taagaattcg tctcaccttc tttctgttat ttgctgattc tgttagacta
     2821 gatataatga aaaaagtata gtgaaagaag gaaaaaacat ggaaatatcc gaaattcgaa
     2881 atctcctgac agaaatgcag gaaaaggtca caagcttcag gaggtctctt tgacttagac
     2941 cagctggaag aagacattgc ggaagcagaa gcaaaaatgg ctgaacctgg tttttgggat
     3001 gatagcgaag ctgctcaaaa agtgatcagt gaaaacaatg caaataaaga aacttatgat
     3061 caattccatt ctcttgctga agaactggaa gaaatcgaat tgatggtgga gatgttgcag
     3121 gaagaacctg atgcagccat gcaagaagaa gcagaaacac gtatcaatgc gctagatgaa
     3181 aaaatgaaga catatgaatt atctatgttg ttagatggtc cttatgatcg aaacaacgcc
     3241 atcgttgaat tgcatccagg ggctggcgga acggagtccc aagattgggg cagtatgctt
     3301 ttacgtatgt atacacgctg ggcagagcag catgggtatc aagtagagac actagattat
     3361 caagcaggtg atgaagcagg aatcaagagt gtgaccctac tgatcaaagg acacaacgca
     3421 tttggttatc tgaaatcaga aaaaggtgtt catcgccttg tacgtatctc gccttttgac
     3481 tcagcgaaac gacgtcatac ttctttttgt tcggtagatg tgatgccaga gcttgatgaa
     3541 acgatcgata tagaaatcaa tccagatgat ttgaaaatcg atacgtaccg agcaagtggt
     3601 gcaggtggtc agcacatcaa taaaacagaa tctgctgtac ggatcactca cttgcctaca
     3661 ggaacagtag tagctagtca ggcacaacgt tcacaattga aaaaccgtga gcaagcgatg
     3721 gggatgttaa aagctaagct ttatcaatta gaagtggaaa agaaagaaca agaagcagct
     3781 gcacttcgag gagaacaaaa agaaatcggc tggggttcac agatccgttc ttatgttttc
     3841 catccatatt ctatggtaaa agaccatcgg acaaacgtag agacaggaaa tgtccaagca
     3901 gttatggacg gtgaccttga tttatttatt gacggctatt taaaaatgca tctacaataa
     3961 gaacccattg ttaaattgaa ataaaattgt aagattttgc aagcatgttg aaacattgcc
     4021 atgctataat agctaaagac tgaaaaagat atggagagaa cgccatgatt gaaatgaagg
     4081 atgtaatgaa gaagtactca aatggtacga ccgctatccg caatctctca attgtcatcg
     4141 aacaaggaga gtttgtctat gtggttggtc cgtctggagc cggtaaatct acattcatca
     4201 aattgatgta tcgagaagaa aaagcttcaa aaggaacttt gaacgtcgca ggtcatgatc
     4261 taatagcaat caaaaaccgc gaagttcctt atctacgcag agaaatcggc gtggtgttcc
     4321 aagactataa attattacca agaaaaacag tatatgaaaa tgttgcgtac gctatgcaag
     4381 ttatcggcag aaaacctcgg gatatcaaaa aacgagtaat ggaagtgctg gaccttgttg
     4441 gtttgaaaca caaagttcga gtctttccta gcgaattatc cggtggggaa cagcaacgtg
     4501 tctctattgc tcgggcaata gtgaatacgc ctaaagtact tatcgcggat gaaccgacag
     4561 gaaatctgga tccagaaaac tcatgggaaa tcatgaaatt attagatcgg atcaatgccc
     4621 aaggaactac tgtcgtgatg gctacacata acagtacgat cgtaaatacg attcggcacc
     4681 gagtaattgc tatcgaaaac ggccgcatta ttcgagatca agcggaaggg gaatatggat
     4741 acgatgatta gaaccttttt ccgccattta ttagaaagta tcaagagttt gaaacgtaac
     4801 ggctggatga cagttgcttc agtcagtgcg gtaacgatca cattgacttt agtcggtgta
     4861 ttcatggcag ttatcatgaa cgcaactaaa ttagctcaag atatagaagg aaacgttgat
     4921 gtctctgtat tcgtagatat cggtacaaaa gacgaagaga tgaaaaagct agaaaaagaa
     4981 ttgaatgact tagatcatgt taaaaaagtt gaattttcaa gcaaagaaca ggaattgaaa
     5041 aaaatccaaa acgaaatggg tgattcctgg aaattgtttg aaggtgacaa taacccgtta
     5101 tatgatgtgt atgtagtaag tgctactgat ccttcttata caaaacaagt agcagaagaa
     5161 gcagcagatt tgaagaatgt ttctcgttct gattacggtg gagcttcctc agatcggatt
     5221 ttccaaatcg ctggtgcagt tcggacatgg ggattagttg cagcggcatt gctgctgttc
     5281 gtagcgatgt ttctcatttc gaacacgatt cggatcacga ttttgtctag acagcgtgaa
     5341 attcaaatca tgcgtttagt cggtgcaaaa aatggatata ttcgctggcc gtttttcctt
     5401 gaaggtggct ggatcggtct gatcggtgcg attcttccga ttttgatcat cacatttggt
     5461 tatgcatggt ttttccaatt gatcaatcca tcattattac gttcccatta ttcattgatt
     5521 catccacaaa atatcgtttg gaaaatcaat cttttaatgg tcggcattgg tgtgatcatt
     5581 ggttcacttg gctcgatcat ttctatgaga cgtttcttga aattctaata ccacaatata
     5641 aataaaatga gaaagttgga cagtttcgtt ccagctttct ttttttattg gctaggagaa
     5701 tagaaggaag cggctttttt ccctttactt taaaaaacaa cttgggtatt cttaagttgg
     5761 tagaagaaga ggtgaagccg tgaaaattat cgttgacaat ttttttacga tccttttgat
     5821 tatgaatatt ttgctttcct ttatcatcgt ttttcgagaa agaaaggaaa ctgctcaaac
     5881 atgggcatgg gtgctcgttt tgatgttcat tccagttgta ggatttattt tatatatttt
     5941 tttaggaaga gggatttcca aagagaaaat ctttgatttg aagatccaag ggaaaatcgg
     6001 taagaacctg gaaattgaag aagacaaaca ggcattgatg cgagggcttt atcctcatcc
     6061 gcctactgga caagtagatg tgaaacaatt gatctatatg ctcacggtat ttgaaagtac
     6121 gctctatacg actaaaaatg aaatcacttt atttacagat ggtcgtgaaa aattcgatgc
     6181 gctgattgaa gatatcaagc aggcagaaga ccatatccac ttccagtatt atatttatcg
     6241 cagtgatgca ttaggtgaag aagttcgaga tgcattgaca gaggcggcaa gacgaggggt
     6301 aaaagtcaga gtccttcttg atgcatgggg ttccacacaa gtttcttcca gcttttttca
     6361 gaatttgaaa aaagcaggcg gagaagtcgc atttttcttt cctctatttg ttccgtatat
     6421 taatcctcgg atcaattatc gcaatcatcg taaaatcgta gttattgatg ggaaaatcgg
     6481 atatacaggt gggttcaatg ttggaaatga atatctagga ttggtaaaga aatttggtta
     6541 ttggcgagac aatcatttgc gtatttacgg agaagcggtg tataccttgc aaaaccgttt
     6601 tttgatggac tggaattctc agcatacaaa agaagtaggc tattctccca aattttttcc
     6661 gtccattcac tctactggtg atatagctgt tcaaatcgtg acaagcggac cagataccga
     6721 acatgaacaa atcaaaatga cttatttgaa aatgatttcg ttagccaaac gggagatatt
     6781 gatccaaacg ccttattaca taccggatgg atcgattcat gaagcactga agttggctct
     6841 tctttcaggc gtaaaagttc atatccaaat acctaacaaa ccagatcatc ttctcgttta
     6901 ctgggcaacg tattctttcg ctgcagagct aatcgagtat ggagcaagga tcgaaacata
     6961 tgagaatgga tttattcatg caaagacgat gattatcgat ggtggcattg tttcagttgg
     7021 ctcagcaaat atcgatgttc gttcatttcg cttggacttt gaggtcaata cactggttta
     7081 tgatgaacga atggcttcac gtgtaagaaa agcttttttt gaagactcga aaatttctac
     7141 gcatctcaca aaagaaatgt atgaaaaccg aggaataatc atcaagatga aagaaggatt
     7201 agcaagattg atttcgccgc tcctttaaaa cataaaaagg acatgagaac catcaacaga
     7261 tggagatctc gtgtcctttt ctaacggggc catttctgag agtacgtcag aaatggagaa
     7321 agcgtctcgc atgagcgaaa ttaactttgg ggtatctctt gttttttggg gaaagcaaga
     7381 aataagctaa tatatgctat taaaagagac acatttcaaa gccagtatag catagaaact
     7441 tttaatgaga tagttttttt tacaattatg agaatttatt ttgttattta atgattttga
     7501 ttcgttttca tcaagtaatg aaaatgataa aataaacata ctttaggatt aaaggagatt
     7561 tctaatgaaa aaacttaaat tattcttacc aataatagga ttagctattc ttttatcagg
     7621 ctgttcaaag tggattgatc gaggagaatc cattacggct gttggctctt ctgctttaca
     7681 gcctttagta gaaacagttg ctgaaactta ccagacagaa aatcctggaa aattcatcaa
     7741 tgtacaaggg ggcggaagcg gaactggttt gtctcaagta cagtcaggag cagtcgacat
     7801 cggaaactct gatttgtttg cggaagaaaa atcggggatc gatgcctcag cattagtcga
     7861 tcatcgagta gcagttgttg ggatcactcc tattgtgaac aaggatattg gcgtaaaaga
     7921 catcagtatg gaaaacctga aaaaactatt tacaggcgag atcaaaaact ggagtgaagt
     7981 cggtggaaaa gatcagccag tagttatatt gaaccgcgct tcaggaagcg gaacccgtag
     8041 cacgtttgaa aaatgggtat tggacggaga aacagctgtt caggcacaag aacaagattc
     8101 aagcggaatg gtgcgccaga tcgttgccga cacacccggt gcaatcagct atgttgcttt
     8161 ttcttatgtt tccgatgatg tagatacgtt gaatgttaac ggtgtagaac cgacagatga
     8221 aaatgtgaca acgaacaaat ggacgatttg ggcatatgag catatgtaca caagagatca
     8281 tccaaaaaaa ctgacaaaag aatttttaga ctatatctta tctgatgaag tacaagataa
     8341 catcgttgga gaactaggtt atatccctgt ttctcatatg aaagtcgaac gtgattggca
     8401 aggaaatatc gttaaagagt aatctttaca caaaatttac aaacttagct aaacttttac
     8461 atgttattaa aaccaaattg atgttcaatt tgtacactga gtgagtatgt taaacctgat
     8521 taagggggag tcatctgtgg aagatgtgaa aaaagtatta ttaacaaaat caaaacgttc
     8581 aaaaatggaa cagcgtggaa aattcatcag ttttttatgt atcgcgctga tcgtgttggt
     8641 tgtattatct attttttatt ttgtagctag taaaggattg gccacctttt ttactgataa
     8701 gatcaatgta tttgattttc tgtttggtac agattggaat ccaagccaaa tcggagcaaa
     8761 tgggaaacca atggtagggg cattgcctat gatcacaggt tcgtttattg tgaccttttt
     8821 atctgctatc gttgccacgc catttgccat tggtgcagca gtattcatga cggagatctc
     8881 tcctaaaaac ggaacgaaga tcttacagcc ggtcatcgag ctgcttgtag gtattccttc
     8941 tgtcgtttat ggttttatcg gtttatcagt tatcgtgcct tttgtcagaa gtatttttgg
     9001 cgggactggg tttggtatct tagctggtac atttgtgttg ttcgttatga tcttgccgac
     9061 tgtcacaacg atgactgttg atgcattaaa ggcagtgcca cggcactatc gggaagcttc
     9121 attagcgctt ggtgccacaa gatggcaaac aatctacaaa gtcgtgctac gagcagccgt
     9181 gcccgggatt ttaacagcag tcgtttttgg tatggctcgt gcgtttggtg aagccttagc
     9241 tatccaaatg gtcatcggta atgcagcatt gatgccaaca ggtcttacta ctcctgcttc
     9301 tacattgacg tccatcttaa caatgggaat cggaaataca atcatgggaa cagttgaaaa
     9361 taatgtgtta tggtcattag cgctgatctt gctattgatg tcattattct ttaatatcgt
     9421 gatccgtatg attgggaaga aaggagagct gaaataatga acgcaaaacg ctcagataaa
     9481 attgctacag gtgttttata tatagtttcg gggatcatcg tattgatttt agcagcttta
     9541 ctactttata tcttagttcg gggaataccg cacatttcat ggtccttctt gaccgaacct
     9601 tccaaagcct accaagaagg cggtggaatc gggatccagc tatttaactc gatttatttg
     9661 cttttgatca cgatgatcat cagttttcct atttctttag gtgcgggtat ctatctttca
     9721 gaatatgctg gaaaaaactg gctaacagat gtgattcgca cgtcgattga aatcttgagc
     9781 tcgttgcctt ctgtcgttgt cggtttattc ggtttcttgg ttttcgttat tcaattccag
     9841 tatggtttct cgattatttc tggtgcattg gctttgactg tattcaactt gcctcttttg
     9901 actagaacag ttgaggagtc tttacaagcg gtccattaca cacaacgaga agcaggattg
     9961 gccttaggtt tgtcacgctg ggaaacagtg ataaaagtgg tggttccaga agcgacacca
    10021 gggattttga ctggtgtgat cctaagttct ggaagaattt ttggtgaagc agctgcattg
    10081 atctatacgg ctggacaaag tgcacctgcg ttagatttta cgaactggaa tccattaagt
    10141 gtttcaagcc cgatcagcat tttccgtcaa gctgaaacat tagctgtcca tatttggaag
    10201 atcaatacag aaggcacgat gcctgatggt gtttctgttt cggctggcgc ttctgccgtg
    10261 ttgattattg ctgttttact gttcaacttc ggcgctcgtg cgattggcaa acgattatac
    10321 cgaaaaatga cttcagccta aggagaagaa gcaaaatgaa aaaatataac tggaatgaaa
    10381 caaatgtcat caccatgcca aaagagaaag aagtagcact atatacggat gatctacatg
    10441 tctggtatgg cgacaatgaa gcaatcaaag gagtggatct ggaatttgaa aaaaataaga
    10501 tcactgcact gattggtcct tccggctgcg gaaaatctac gtatcttcgt tcgttgaatc
    10561 gaatgaatga tgggatcgct aatacgaaaa tcactgggaa aatcatgtat caaggcgtag
    10621 atattaatgc gcctcaagtg gatgtttatg agatgcgcaa acggattggc atggtttttc
    10681 aacgtcctaa tccgttcagc aagtcgattt atgacaatat tacatttgct ttgaaacagc
    10741 acggcgaaaa aaacaaaaag aaattagatg aaatcgttga aacaagcttg aaacaagctg
    10801 ccttgtggga ccaagtaaaa gacagcttgg acaaaagtgc cttggcttta tctggcgggc
    10861 aacagcaacg tttatgtatt gctcgtgcga tcgcgatgaa acctgatatc ttgctgctag
    10921 acgagccggc aagtgctttg gaccctattt cgacaggaac ggttgaagaa acacttgtaa
    10981 acttgaaaga agactatacg atcatcatcg tgacgcataa catgcaacaa gcagctcgta
    11041 tcagcgacta tactgccttt ttctatttag gaaaagtaat ggaatatgac aaaactaaca
    11101 agatatttac cagaccaaaa attcaggcaa ccgaagatta tgtgtccggt cattttggtt
    11161 aagacggagg cctcaacatg acaaaagaga ttatttcatc taaagatctt catctgtatt
    11221 atggaaagaa agaagcacta aaagggatcg atctaagctt caatcaaggg gaaatcactg
    11281 cgatgatcgg tccttctggt tgcggaaaat ccacttatct gcgttctttg aaccggatga
    11341 atgacctgat tccgaatgta acgatcaccg gaagtgtttt atataaagac aaagacatct
    11401 atggtccaaa aacagatgtt gttgaactaa gaaaagaaat cggcatggta ttccagcagc
    11461 caaacccatt tcctttttct atctatgaaa atgtcatcta tggattgaaa ttaaaagggg
    11521 aaaaagataa aaaagtcctt gatcaagtag ttgaagaaag cctgaaggca gcgtctgttt
    11581 gggatgatgt gaaagacaaa ttgcataaaa gtgctttatc tttatccggt gggcagcagc
    11641 agcgtgtatg tatcgcacgt gttttggcag ttaatccgga aatcatctta ttggatgaac
    11701 caacaagtgc gcttgatcct gtttctactg ggaaaatcga aagtatgctg ttagaattaa
    11761 gagagcggta tacgatgatc atcgtgaccc ataatatgtc tcaagcttca cgtatttcag
    11821 ataagacggc attcttttta gatggtcact tgatcgaata caatgatacg aagaagatat
    11881 tcatgaatcc agaaaaacaa gagacagaag attatatttc aggtcgattt ggataaggag
    11941 gaaacggaat gctacgtaca caatttgaag aagaattgct gaatctccat aatcagttct
    12001 acgaaatggg gatgatggtg agcagcgccg tgcacaagtc agttcgtgca tacatcaaac
    12061 acgataaaat cttagcccaa gaagttatcg aaaacgatat aaatatcaat aacatggaaa
    12121 cacgcttgga gaagaaaagt tttgaaatga tcgcattgca acagccagtt acaactgatt
    12181 tgcgtatgat cattacggtc atgaaagcta gctcggattt agaacgtatg gccgatcatg
    12241 cggtgtctgt tgctaaatcc acgattcgcg taaaagggca gacgagaata ccggaaatcg
    12301 aaaaagagat ctccgatatg tccgattatg tgaaaaaaat ggtggacaac gtcctagttg
    12361 cttatgtcaa aacagacgaa aaagacgcac gtacgatcgc caatatggat caaagagtca
    12421 atgactattt caatcgaatc tataatgcaa caatcaaaaa tatgcaggaa aatcctgaga
    12481 cagtcatcag tggaacagac tacttgaatg ttgccagtta tctagaacgg atcggagact
    12541 atgtgacaaa catctgtgaa tggatcgtct acttggctac agggaaaatc actgagttaa
    12601 atacgaacca caatgagatt ttctaaactg aagctgtgat tatgccgttt gtttaggaaa
    12661 agaggttgtg aaatcagcgg ttgtgaaaaa gctgttctgc atcaagtaat aagaacagtc
    12721 aaacaaaaaa tagctcctca tatttttcgt ttggctggac ttattaccgc agatgcaagc
    12781 ttttgaacac cgtttattcg ggaaagggtt tgtgacaaaa catgtcacaa acccttttca
    12841 tcatttcata gaaaggataa agaatgcaaa aaaaaattag catcatcact atatttttta
    12901 tttgtgtgct tacttcatgg gttattcagc atcaagccgc tttatatgaa gtacctgtca
    12961 tcgagataga aaaagtacga ataaaagagg atcatcaatt gattacagga aaactgatga
    13021 acacggatca aaaaggagaa tcgatttatt tatcggttcc ttatcaaaaa aatcaaatag
    13081 aaacagcaaa tataaaagcg agacaaatgt atctagtcca aatgcatgga gatgaatggc
    13141 agctagtagc aaaaaaaaat gatggctggc ttttcttctt cacaagtatc ttcattggcg
    13201 ccatgttgct tattggtgga aaacagggtc tatttgcttt aggaagtctc atcatcaata
    13261 tcgttttact tttacttttg ttgctcatct ttactaaaac agaagggatt tctctattaa
    13321 atatatctat cctgttcgtc gttataggga tgattattag catattggct ttagatggat
    13381 ggaatagaag cagtataatc aaaatttcag ctgcagttac gagtgttttt ttagcatttt
    13441 ttatttgtct tttgacaatg aatcattgga aagaccaagg actgcgctat gaggaactga
    13501 attatctgac acgaccgtat cgttctgttt ttctttctag cgtactgatt ggaacagctg
    13561 gaggaacact cgataccgtt atcacagtca ttgcgacatt agaagaattg actaagcgac
    13621 aaccagaaat aacagcgaaa cagttatggc tttcaggaag gcaagtgggt cgagatgtga
    13681 ttggctcgat gaccaatatc cttttgttcg tctatatcag tgggtcagtt ccatcgcttc
    13741 ttttatattt aggaaatcaa tggtcgttca aagaaacgat agaacagcat ttatcattgg
    13801 aatttttacg cgtcattgct ggcagcttag gaatagtatt atcgatccct atctcggtcc
    13861 tcttttttct gactgtaagg aggctgaaac gatgaatgta cttattatac ttgttgggat
    13921 tttagcaatc agcgtgctac ttattggcgg tacgcaaggt atgtatattc ttttaggctt
    13981 attcatcaat ttagggatct ttttcctgtt gttattcggt tatcatcaga agtggccgat
    14041 actggcattg tcggtcatag gatttttatt gattgctgtg gttatcttat tttttatcaa
    14101 cggttataat ctaaaaatgc gtgcagcttt tgtgagtata ctgatctttt tattttgttt
    14161 cctactattg cttcctatca ctgatttttt agcaatccaa ggttttacat cggtagaact
    14221 agaagaatta tctggcttag ataagacttt agcgattgat tttagattac ttacgagatc
    14281 gttgcttctg attagtctta gtggtgctgt tttagatgcg agtgtagcaa tttctagtgg
    14341 gacattcgaa gtatatcagg caaatccgca attatctttt taccaattgc gccattctag
    14401 tttcgctatt gctaaaaaaa tattggcgtc aacagtaatg acacttcttt ttgcattcat
    14461 gggaagttct ttagcgttga tcctctggtt tattgattta gacattcctt ttcagcaagt
    14521 gatcaatgaa aaatcatttg ttctagaata tacgatggct attcttacca cattgtccgc
    14581 actacttgtt cttcctttaa cctgcgtgac tacagcatat ctctttacaa aaaagaaaga
    14641 acaactcaag atggagtaaa agttacaact taagtcctat gacattttta ctgaatcagt
    14701 gcataatatg gatgtaagga aaagcttgca aacaattttg cttaaggagg caatttccat
    14761 gaaagaaaga gaacgtattt tagatttagt gaaaaaagga atcctttcga ctgaagaagg
    14821 cttagattta ttagaaagca tggcaactga aaaagacgaa aagcaaatca aaaaagaagc
    14881 agacaaggta actgctagtc ataaagaaaa agatcaggca agtcaattga tcgataaact
    14941 tgaaaacggc gaagaagaaa tttctgagcc agttgatcca aaggaaagag aaagacaaga
    15001 tcaagaaaac ttagaaaaga tcttagatga actagcaact gaagcgaata aaacttcggc
    15061 gcgtctagat gaagtaaatg ctcaattcgc tgacaataag gcagcacgca atgagaaaca
    15121 ggaagcattg atgcagctga atacaaaaga agaattaggc gagttgacag aagaagaatt
    15181 agcacagcgt cagacgttag aagcagaaat caaagaatta gaagctagtg gcgatacact
    15241 gttagaagaa caattgaaat tagaagcaga attaaaagat atccgtaaga atcaatggtc
    15301 tgagaaaaaa gaaacgttta ctgataagtt tgaattgcca gatgattgga aagatcaagc
    15361 aacagataca ctgaatcaag taggcgaaaa aatgtcagaa gctggatcac agttaggcaa
    15421 attcttgaag aaaacattcc aaactgtttc agaaacagtg aatgacaaca tggaatggaa
    15481 agatgtcagc ttacgtgtcc ctgggattgc gacaacgaaa tttgagcatg aattttacta
    15541 tgaagctcct gcagcaagta tccttgatat caaggcagca aacggaaacg tgacgttaaa
    15601 aacgtgggac agcaacgatg tgaaagttga agctaagatc aaactttacg gaaaaatggg
    15661 tgctgaacca tttgaagcat tctctgaacg gagccagatt gaggttaatg aagatcatat
    15721 ctccttccaa attccaaata aacgtgtgcg tgcagacctt gttttctact tacctaaacg
    15781 agtatacgac cacgcagcta ttaaacttct aaatggcaac atcatgattg aaacattgga
    15841 agctaaagat atttatacaa aatctacgaa tggtaacatc atcgtggacc aattaactgc
    15901 aacgatgctg gaagtagaag gtgtaaacgg caatatcgat attcgaaacg ggaacatctt
    15961 agattctatt atcgaaacag taaatggtac agtgacattt ggtgcgacag cagaaaacct
    16021 ttctgtgtct ttggtaaatg gtgatgtacg tcttacgatt aaagaagata atttgaaaaa
    16081 agtagaagca agttcagtga acggaaatgt caaagtcgct ttaccagatg gcatcggttt
    16141 ggaaggacat gctaaaacaa gtcttggaag tatcaacagc cgtctgtcca attacgaagt
    16201 agttcgtgag aaaaaagaac ggacaaacca aatgttgcaa ttccgtcgtg tttcagatgg
    16261 tgaaatcgcg caagtgcaac tttcaacgac aacaggaagt atctatttaa aagatacaga
    16321 taaataaaga ggttgtgaaa gagatgttca gccttgagca ataagaagcg attttgtaaa
    16381 atagcttccc ctattttaca aaattgtgcc ttattgccga tagtctatct cttgaacacc
    16441 gtttaccaag agattatgaa cctgccaatt atcgccgagt cacaaggaat aatttcagaa
    16501 aaagctcgtc acgttttatg aatatggtaa tttgtgaccg agaattttgc agaaagacac
    16561 cgattaagaa gaaggttgtc acaacgattt tcttcattta aaatagtaga acagaggtga
    16621 gacatgatga aaaaaaagtt gacaaaatct aataaaaatg ttgttttgac agggacttta
    16681 gcaggaatag gagaatacat cgggattgat ccgactgtta tccgtgtgct ttatgtattc
    16741 ctgagcttag ttgcttttgg aagtccaatt attttataca ttttattagc tttgattatc
    16801 ccatctgcgc gttctgcacg aacaaactat ggacataaca atgcatatta tgaaaaaaac
    16861 aattatcgtg aacaaactcg tgaacaaaca aaaaaacgca aagaagcgga aaaaatcgat
    16921 gatgacgaat ggagtgactt ttaatgtcgt actttcaacg tattttagtt aatacgttaa
    16981 cattcatatc attggctgtt ctttttcctg cgatggtcca tgtgagcagt atttggacgg
    17041 ccgttcttgc cagcttcgta ttgtctgtgt tgaatatgtt tgtcaaaccg attttgacga
    17101 tactgtccct gccctttaca ttagtcacat tgggattgtt tacgtttgtt atcaatgcag
    17161 cgattcttaa aatgacctcc ttttttgtgg gagcagaaca ttttagtttt tcttcattcg
    17221 gtgcagcgtt gttagtagct atcttgatgt cgcttgtcaa tgtaattgtc agcgaacata
    17281 acagtagaaa ataaaagaaa gctgtgccga actatcggcg cagttttttt atttagaatc
    17341 aggaatgcgt tttctattac attggtaatt aaataaaaac gtttttttta attggtagta
    17401 cttttggcta gacaatgata aaatgataga gaacactcta atagaaatga ggtagcatga
    17461 atgtcagaag ttgtaaaagt tagccaatta gtgaaaaaat tatctttgga aatcgtgaca
    17521 ggcgacgaga aaagcttaga ccgtgtcatc gtaactgggg acatctcacg cccaggacta
    17581 gagttgactg gttattttaa ttattattcc cataatcgtt tacagctatt tggcagtaaa
    17641 gaaatcagtt ttgcggaacg gatgatccca gaagagcgtt tgatcatcat gagaagatta
    17701 tgtagtgaag atatgcccgc atttattatt tcaagaggat taccagctcc agaagaaatg
    17761 atccaggcag caaatgaaaa tgggatagct gtactgcgtt ctcccatctc aacttctcgt
    17821 ttattaggag aattatccag ttatttagat ggtcgccttg cccctcgaac aagtgtccac
    17881 ggtgttttag tggatgttta cggattaggt gtactgattc aaggtgacag tgggatagga
    17941 aaaagtgaaa cagcgttgga gttgatcaaa cgcggccatc gattgatcgc agacgatcga
    18001 gtagatgttt atcagcaaga tgaattaacg gtgatcggag aaccgcctaa aatcctggaa
    18061 catctgattg aaatccgagg aatcgggatc attgatgtaa tgaatctttt tggtgcgagt
    18121 gcggtacgtg gctctatgca agtccagttg gctgtttatc tggaagcatg ggcaaaagat
    18181 aagaaatacg accgtctggg ctctgaagac acgaccgttg aaatcgcgga agtgggcatt
    18241 ccacaaataa aaatccctgt caaaacagga cgaaacgttg caatcatcat tgaagtagcc
    18301 gcgatgaatt tccgcgcacg tacaatgggc tttgacgcaa cgaaaacatt tgaagaaaga
    18361 ttaagtcgct tgattgaaga aaactcaggc aacgactagc aaggagtgac aatatgttag
    18421 cacaaatcaa tcgagttgct tttgagcttg tagggattcc tatctactgg tatgcattga
    18481 tcatcgtatc aggtatcatt atcgctatgt ggcttagcag cagagaggct gttcgggtag
    18541 gattaaaagc agatgatgtg acagatttta tgttgtgggg attgcctatc tccattattg
    18601 gcgctcgctt atattatgtt ttatttgatc ttccccagta tatagctgat ccaatccaaa
    18661 tattcaatat ccgttcagga ggcttagcta tttatggcgg actgattgca ggcgcgatca
    18721 cgctgtattt ttttactaaa tatcatttta tttctttgtg gactttttta gatatcgctg
    18781 caccaagtgt attattagca caggcaatcg gtcgttgggg aaatttcatg aaccacgaag
    18841 catatggacc agacacgacg cgaagcttct tagaaagtct gcatttaccg caattcatca
    18901 ttgataatat gtatatcgaa gggatctatc gtcaaccaac atttctttat gaatcacttt
    18961 ggagtttgat tggatttatt ttgcttttgt ttttacgaaa gaaaccacac cttttgaaaa
    19021 aaggcgaagt ccttttatgt tatctaatgt ggtatagttt cgggcgtttc tttattgaag
    19081 gattacgtac agacagtctt tactttttag gtgtgatccg tatttctcaa ttattatctg
    19141 ctgtgttatt tgtcggtaca ttgattctat ttatttggcg cagaaaaaaa ggaaatttac
    19201 cggattatga ccgctcatta ggaaaaaatc aacaaattat ctgattttta gagcgttaaa
    19261 aatgaaaaaa atttcaggag tggtaaaatg aattatagag tagcggtttt aggaccaggc
    19321 tcttggggta ctgctttggc acaagtcctc gccgaaaacg gacatgacgt tcggatttgg
    19381 ggacatcgtg cttcacaagt agaagaaatc aacacccaac acacgaatca acgttattta
    19441 ccagatcatt atctacccga ttccatccaa gcattcacca atatggaaga tgcagtaaaa
    19501 gacgcagatg cggtgctatt cgtcattcca acgaaagcaa tacgttctgt tgcaaaagaa
    19561 ctagtgccta aattgaatac aaagccagtg atcatccatg caagtaaagg attagaacag
    19621 ggaacacata aaaggatctc tgacatcttg ttagaggaaa tcccagcaga aaaaagaaaa
    19681 gctgttgttg ttctttctgg accaagccat gcagaagaag tagcaaaaca tgacatcact
    19741 acgatcacag ctgcaagtac agatgcaaaa gcagcaaaat atgtacaaga gctgttcatg
    19801 aataattatt ttcggatcta taccaatcct gatgtgatcg gtgtagagat gggggcagct
    19861 ttgaaaaata ttattgctat tggtgctggc gctattcacg gcttagggtt tggcgataat
    19921 gcaaaggcag caatcatgac gcgcggatta gctgagatca gccgtttagg tgtagcaatg
    19981 ggtgccaacc cgctgacctt tatcggatta agcggaatag gcgatctagt cgttacttgc
    20041 acaagtgttc attctaggaa ctggcgcgca ggcaacttgt taggacaagg gcagccccta
    20101 gaagaggtgc tagccaatat gggaatggtc gttgaaggtg tgaatactac ccaagcagcg
    20161 atggaactag cccaaatgat ggaaatcgaa atgcccatca ctcaaacaat ctatgaagtg
    20221 ctgtatgaac aaaaagaaat caaaacagcc gcaaaagaaa tcatgttacg tgatggaaaa
    20281 aaagaaaatg aattctcatt aacaattttt tgaaggagcg aatgtatatg aaagttaaaa
    20341 aagcagttat tccagcagca ggattaggaa caagattttt accagcaaca aaagctatgg
    20401 caaaggaaat gttgccaatt gttgataaac caacaatcca gttcatcgtt gaagaagcgt
    20461 tgaaatcagg tatcgaagac atcttgatcg ttacaggaaa agctaaacga ccaatcgaag
    20521 atcactttga ttcaaatatt gaattagaaa caaacctaag tgaaaaagga aagaccgaat
    20581 tattgaaatt agtagaagaa acgacagatg tcaacttgca tttcatccgt caatctcatc
    20641 caaagggttt aggacatgcc gttcttcaag caagagcctt tattggaaat gaaccatttg
    20701 tcgttatgct aggcgatgat ataatggaag acgaagtacc attgacacag caattaatcg
    20761 atgattacga acgtactcat gcttctacga tcgccgttat gcaagttcct catgatgaaa
    20821 cttcaaaata cggaattatc aatccaggag aagtattgga agatgggtta tacaatgtca
    20881 aaaactttgt tgaaaaacca gatccaagca aagcaccaag tgatttagct attattggac
    20941 gttacttatt gactccagaa atttttgatg tattgtccga gcaacaacca ggagccggaa
    21001 atgaaatcca actaactgac gcaatcgata cattgaataa aacacaacgc gtatttgccc
    21061 gtaaattcac aggtaaacgt tacgatgtcg gtgacaaatt tggttttatg aaaacaagta
    21121 ttgaatatgg tctaacacat ccagaagtag gtgcaccttt acgtgagtac atcctgtctt
    21181 taggtaaaac attagaagca gaaggaagca agatcgatca taatccaaaa ggaataacag
    21241 aaatcaaaga agaaaagaaa aaataaccct tttttctaac gagcttcagc tgctaattga
    21301 ataaaaagta gttgaagctc gtttttattt gtttaaaaaa cacgaaaatg ctatccttat
    21361 agaaaagaga gacaagaagg gagtttgatc atgtcaggag gagaaattgc aggattgatc
    21421 gcagcaatcg cctttgcgat acttgtcgtt tttatcgttc gtgtgttgtt gcaagtttct
    21481 aaaactttag aaaaagtaga tcagactgta gcagaagcaa atacgacgat cgaaatcgtc
    21541 acaaaagacg tagatctttt gtctagacaa gtagaaggct tactggtcaa aagtaatgaa
    21601 ctgctcttgg atatcaatgg aaaagtcgca acgatcgatc cattgttcac agcagttgct
    21661 gatttaagtg aaagtgtatc agaacttaat cattctagta aaaacatcgc gacgaaagtc
    21721 ggcggaatcg gtaaaacgac agctaaagcg acggttgccg gaaaagtcgg tgggacagcg
    21781 atgaaatttt ttaaaaacaa aaaacatact gaaacgacag gaggaaaata aaaatggcta
    21841 aaaaaggtgg atttttatta ggtgcagtag tcggaggcac agccgcagca atcgccgcgt
    21901 tattgttagc accaaaatct ggtaaagaac ttcgggatga attagctgcc caagcggatg
    21961 atttgatgga tatggcttca gattacacag atatggcgaa agaaaaatcg actgagttaa
    22021 caggtattgc aaaagaaaaa gcgcaagatc tttctcaaca agcaggtgat ctagcaggta
    22081 gtgtcaaaca aaaagtaagt gaatcagcag atgaagcagg aagtgaatct gacgatatct
    22141 tgttaaatct taaagaacaa tcagctgact tatctaatcg tttcaaaaaa acggcagaag
    22201 atatgaaaaa acctgcagat gaattaggaa gtattgctaa agatacttca gaagatatca
    22261 tgattgatgt aaaagacgct gctggtaaag caggagatac tgtcaatgaa ggaatgtcag
    22321 aagcaaaacc agttgcagat gactcaaaag aaatgatgaa aatggcaaaa gaagatgcga
    22381 aagaagctaa aaaagaaata aaagatacaa tgacagattc atctacaaat cactaaaatc
    22441 acaaatttat agaataaaaa ggtgggagcg gactagctct cacctttttt actcttattt
    22501 ttctaaaaca agtaattctt ttggtgtagt cgtgaatggc acacagccat cgcttgtcac
    22561 atatacacag tcttcgatac gtacccctac tttttcagga atatagattc ctggttcaag
    22621 agagaagcac atcccttctt cgattactaa gtcatttcct tctacaaggg aagggaattc
    22681 gtggacggtt gttccgatcc cgtgacctaa acggtgattg aaatattcgc cgtagccata
    22741 gctgttgatc acgtttcgtg cgatttgatc aagttcacct gctgtcacac ctggacggac
    22801 ggcttcttgt gcggcaagct gtgcttcaag agtgatgttg tagatttttt cttggaagtc
    22861 ggttggtttt tgataagcaa ttgtacgagt agcatcactg caatagccat tccaaacaac
    22921 gccaagatcg aataacacaa gttcattcgg tgaaatcttc gtatttcctg gtgtaccatg
    22981 agggcttgca gcatttgctc ctgcaaggac taatgtgtcg aaagacattt gtgaaacacc
    23041 ttgttttttc aattggtatt caatttctgc aatgatcgct tgttcagtca cgccttcttt
    23101 cactgcttta aagccaatct caaatgcaac atcggcccat ttaccagctt cgatcagttt
    23161 gtctttttcc tctagagttt tgatcagttg tagacgttgg atgatggaag ttagatcagt
    23221 gtaagaatcc gttcccgtca agatatcaga gatagcatcg aagcgctcga cagtcaaggc
    23281 gttcttttca atccctacat tgccaggctg accatagcga gcacgaagtt cttttccgat
    23341 gatctcccaa gggttttcgc tgtctaaata gccacggaca tcatacgccc aactactgtt
    23401 ttgagcatct tcaacttcca aagcaggagt gaaaaggaaa ggatcttgtt caagagggat
    23461 aaaaagagct agtacacgct catgaggatc actttcgtat ccggaaaagt aagcgatgtg
    23521 gccagggtcg ctgatataag ccaattcgac ttggtgttgt gccatccatt gacgtagttc
    23581 attgattttt acgttattca tttggcaacc tactttctaa attaattttg tattcaataa
    23641 ttgtaccact gatttattaa aagaccaacg ttctaaaaac tgaaaaaagt aaaaattttc
    23701 atgaaaacag agataaaacg gttgcaattc ttgagattat ttgatattct attttagaaa
    23761 tgtaagacgt attttcagaa aacatataaa atatgaatag gagaacacaa atggaaaagc
    23821 aaacaatcac tatctatgat gtagcgagag aggcaaatgt ttcgatggct actgtttcac
    23881 gtgtcgtaaa tggcaatcca aacgttaaac cagcaacaag aaaaaaagtc ttggaagtta
    23941 tcgatcgctt ggattatcgt ccaaacgcag ttgcgcgtgg cttggcaagt aagaaaacaa
    24001 caacagttgg tgtaatcatc cctgatgtca gcaatatgtt ctttgcttct ttagcacgtg
    24061 gtatcgatga tgtggcaaca atgtacaaat acaatattat cttagcaaat tcagatggca
    24121 acgaccaaaa agaagtcaat gtattgaata acttactggc taagcaagtg gacggcgtga
    24181 tcttcatggg tcaccatatc acagacgaaa tccgtggaga attttcacgt tcaaaaactc
    24241 ctgttgtatt agctggctct attgatccag atgaacaagt aggtagcgta aacattgatt
    24301 atgtatccgc aacaaaagat gcagtaaaca aattggcaaa aagcggcaat aaaaaaatcg
    24361 cttttgtcag tggtgcgttg atcgatccca tcaatggaca aaatcgtctg aaaggctata
    24421 aagaagcatt aagcgaaaat aacctaccat atagcgaagg attgatcttt gaagcacaat
    24481 acaacttcaa agatggtcta tctctagtcg atcggatcca taatagcggt gcaacagctg
    24541 tctatgtttc agatgatgaa ttagccatcg gtattttgga tggtttatta gaccgtggcg
    24601 tgaaagtacc ggaagaattc gaaatcatca caagtaacaa ttcactactg acagaagtag
    24661 ctcgtcctcg tttaacaagt atcacacaac cattatacga tttaggggcg gtatctatgc
    24721 gcctattgac gaaaatgatg aataaagaag aagtcgaaga aaaaacgatt atcttgcctt
    24781 atggaatcga agaaaaagga tctacaaaat aataaaagga caaccattca gaggttgtgt
    24841 gcctgccgtt taactctgcg ttacaaggag caatttcaaa aaacagcatc tcatgtttga
    24901 tgaatattgt gactcgtgat tcggagttgg caggagaaca ccgtttattt gggaaaaggg
    24961 gttatgacaa aattttgtta taatcctttt tttgtgtctt aaaaaagaga gaaagtggtt
    25021 gaaattacat ttatcgtata taaaacaatc cgtgtataga tgctgcattt tgttcaccac
    25081 catcaaaaaa ataagggact gtgacaatac tattgtcaca atcccttatt actgtgtatt
    25141 ctattaaatc aatcatcttt ctcatctgat tctttagttg cttgattcgc tcttgcataa
    25201 gctgaagcgg tgttccagta atctgtatag ttttcatcgg taccgccaac gccaaattta
    25261 aaggcgcttt tcttcggtcc atttttcgcc catagcgatt caactgtttt tcctggtgtt
    25321 tggatggatt ttttattgac agtaactttt ccaggtgttt gaccagtaaa tccagatact
    25381 tttttcttca tcacatcaga tgataattcg aatttctcat cagtgccaaa aatgtcaggt
    25441 tttgcttggt aagcttgatt gatcagattg gcaagataat tagccgaacg tatccttgct
    25501 ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc tactgatggt aatagacgga
    25561 gtagagacaa ttagccatga atctcggtat tcattggtag aaccagtttt tccgacccaa
    25621 tctgcttttc ctagatttcc gtttaagctt gagatggcat ctttgaatgg tgtagtgatt
    25681 tttgcattga tcacactacg catcatgtca ttcatgatcg atgccgttgc ttccgaatag
    25741 atccggacag agttgctttc gtgtttataa attacatttc ctgcgttatc tgtgatggaa
    25801 tcgatgatat agccttcttc atagacgccg ccgtttgcta aagcctgaaa tccgttggtc
    25861 tgttgaagcg tcgtgacatc tactgtacct aaaggagcag attcaacgcc ccaattgtca
    25921 tttgccgggt agttcatctt tgccaagtag tgctcgtaag catattgttt cgatccaccg
    25981 gaattcaaca catcttgata taagtgataa gccgggatat tgttggacca ttctagtgac
    26041 tcacggacgg tttggaaggt gttcgatcct tcgttagtag cattgacgat tttctcgcca
    26101 gcattttctc cttcttgcca agttgttgca taatctgata cacgggattc tgatccgata
    26161 agccccatgt cgattgcagg tccataaaca agaactggtt tgattgatga gccagcttga
    26221 cgttctgtat cgaaggcatg gttattttga ttctgactgt aattccgtcc gccaacgaat
    26281 ccgtagatgc ggccagttct gttatccatc aatacatttc cggtttctac ttgactgcca
    26341 gcaccatcat ctaacatgta tccataattt tcaacgccat tttgcaatgc agcatagaca
    26401 tctttatcga ttgtcgaatg gattgtatat cctttatttt ggatcgtttg ctgtgccttt
    26461 tgataataag cgtcactgac cgatgaatca gacatatcag cgttgtcttt ttcagccaat
    26521 tgttgtgcaa tgattcttgt tgcttcattc atcacagtat aatacaagaa ttctctgtca
    26581 gtctgttctg cgatttgttg tggtagaaag tcttttgtaa ggtcataggc ttttgcttct
    26641 tcgtattgtt ctttcgtaat ttggccttca cgatacatac tgaataaaac aaaatctttt
    26701 ctggcaaggc cggcagaaag atcttctttt agcgcgcctg tgttcgtgta tggactatac
    26761 gtgatcgggc tttggggtaa tccagcgata taagcagctt gcggcagcgt cacatctttt
    26821 gcatctacac caaagattcc ttttgcagct tcctggactc cagcaatgtt ttgtccctta
    26881 ttattacgac caaatggtga gacattcaaa tacgtagcaa tgatctcatc ttttgaaaag
    26941 tatttttcta cttgtgcaga aagaatgatc tcgtttgctt tacgtttgaa tgttgtttca
    27001 tctgtaagga tctgctgttt gactaattgc tgtgtcaaag ttgatccacc agaagagcca
    27061 ataccagtag cttcagatac taatgctcgt aaaacagctt taggaacgta accttggtgt
    27121 ttatcgaagt attcatcctc tgtgctgata attgcagttt tcaataaagg tgaaatctta
    27181 tcagatgaaa tagtcgtgcg catcagatct gtctggattt ttgatatctc tgtattatct
    27241 gcataaacaa tctttgacgt ttctgtaatg tttcctagtt ctgtctgcag tgctttttta
    27301 gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc cagcgcctat tccaatacct
    27361 agagctgcga acatcaaaag aagaacagta ataaaaacag tcaatgattg aaaaactcta
    27421 aaaataatct taggaacaaa ccatttttct ttttttcttc tattctgttt ttttcgagta
    27481 tttttgcgtg gcaaaagggt cacctcaact aatggtttta tttcgctaat tatagcaaat
    27541 aatagaacaa aaaaacagac aaggctgttt ttggcaggaa tttctttttt aaaagagtta
    27601 aaacgtgaga aaatgatctt tattataaaa gagttcttaa gcttttcttt agagaaaatt
    27661 tcagttacta tggagaacag ggcaaagaaa aatcaatcat tttctttgta tatcatctta
    27721 ccaatatata ggaattgtta gtaacaattg cctttcaagg aggaatccat ccatgttatt
    27781 aggctcacac gtcagcatga gcggaaagaa aatgctgtta ggagcagcag aagaagcagc
    27841 cagttatgga tcttcgactt ttatgatcta tacaggcgcc cctcaaaaca ctagaagaaa
    27901 agccatcgaa gagatgaata ttgatgccgg aaaagcgtac atggcagaaa aaggtctaac
    27961 aaatatcgtc gttcatgccc cttatatcat taatttgggg aatacgatca aaccagaaat
    28021 gtttcctttt gccgtccaat ttctgagaga agagattgtt cgtgcggaag ctttaggcgc
    28081 tactcaaatt acacttcacc caggtgctca tgtaggggca ggaacagaag ccggaattgc
    28141 tagaattatt gaaggcttga atgaagtttt gacaaaagaa cagacggcac aaatcgcttt
    28201 ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca tttgaggaat tagcgcaaat
    28261 catcgatgga gtaaaattga atgaaaaatt atccgttact tttgacacct gtcatacgaa
    28321 tgatgccggt tataatatta aagaagactt ttcaggtgta atggaagagt ttgataagat
    28381 catcgggtta gatcggctta aagtactcca tatcaatgat tctaaaaatc cgcaaggttc
    28441 gcataaagac cggcacgcca atattggttt tggaacaatc ggattcgaca cgctgaataa
    28501 gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc ttggaaactc cctatgtagg
    28561 caaagataaa aagaaagctt acgcaccata tggaaaagaa atcgccatgt tcaaagcgca
    28621 aacattcgat ccagaagtgt ttcctgagtt attagcagaa gaagtgatac tctaagattt
    28681 tctatctgac agcagcaaaa aaggaccagg cacattgcct tgtccttttt tgtttaagaa
    28741 acaaaggagt actttttgca gtctttttca tcatgcatct tctcccccct ctttttaaat
    28801 ttagacaaac aaggtatact ggtagaaaag taaaaaaacg aaaagtttca caagcaagtg
    28861 gcaaaacgtg attggaagga cgaaaagatg ctgaacactt taaaaccctt gatcaaccaa
    28921 ctaaaaaaga aacatacata ctttattctt ggcatagccc tattatttac aattattcgt
    28981 ttgtatttat atagtaaagc aatctatgga acagacatgt cggcaaccta tgatgaccag
    29041 ctgctgattc gctatgctca aaatattttg gacggtaaat ggctagggga gtatacgact
    29101 acaacacttt ccaaaggaat ctcctacagc ttatttttag tacttgcaca taaactgcat
    29161 ctttcttatg cagttttgtt cggcatgttc agtatttttg ccagtatatt ggttgcactc
    29221 gcgtttaaac cgattgtgaa gaacaagtat cttcttttaa gtatctattt ttattttttg
    29281 ttttctccga ttagttttac acatgaatat tccactcgaa catatcgtaa tgccctcgtg
    29341 attccggctg ttgagatcat tatcgcttgt ttattagcta tttattaccg caaatacggt
    29401 tctagcagaa aattgctgcc atggttttta gggctttctg tcgcttttcc tttcttttgg
    29461 ttcattcgcg aagattctat ctggcttctt ccttttactg tagtagcttt gggtatcagt
    29521 ttcgtccaga tcttgctagg gcaagacttt tctttcaaag aagtaaagaa gttaaaaaaa
    29581 cgactctttc ttctctcgaa acgggaatgg ctgaaacttt cattgttttt acttcctata
    29641 gttggctttt ccatcgtcta tcaatggatc gaatataaaa atgagacaac atacggtatt
    29701 gcaaccatca atgaccgttc cagtggagaa tttggacgtt tgatgaaaca actgatccga
    29761 atagatgatg aaacgaactt gaataaagaa aacagtgaga tatgggtctc acatgctgca
    29821 ctaaataaag cattagccgc atctcctaca ttccatactg tttctacccg tattgacgaa
    29881 ttatatacga ctcacgcgtg gactcaaggt ggaaaagtaa aagaattacc cggagatatc
    29941 atcttttggg cattgcgaga tgtggtaagc gaatctggtt attaccaaaa caatgcaaaa
    30001 gaaaccaatg atttttggaa aaaagtcaac caagaattag aagaagccta tcacaaggga
    30061 acaatcaaaa agaaaaaaga gttctatctg atgggatcag gagatggaaa gacgatgagt
    30121 gatctgccaa tcgtattcga tttccttcaa tcaggatacg cttatgcatg gcgatataaa
    30181 gatttccatc aaggaggaaa ttatagtttc ggaacgcaag aacaagtcaa acaggctcaa
    30241 gaattattaa accttccgct aatggagaat tggatcgata gtaatgaacc gagaacacct
    30301 aatttccaat taacaaaatc agcaaaagtc gcgaatgtca tcataaaaat ttaccaagtt
    30361 ctggctattc ccgtgctgat tgcttcaata gcagggatat tacttatgat actaggaacc
    30421 gtatttgcaa aagctgatcg tgcctattat agttcctctt tgattgtggt caccggatta
    30481 atactgacac aattggtttt tcttttcggt gtctcatggt tttgttctta tgcgccagaa
    30541 aaaaaggact attttttaag tgtctacact ggtgcaggcg tgccattaac gcaattcatc
    30601 gtagtaacaa gcatgctagt tttcagtaaa ttaaatctga aaaaagcttt atcttctaaa
    30661 taaaaagacc cttggtagct agatttttaa cctaactatc aagggtcttt gtgtggaaaa
    30721 atcagttttt attcgatttc ttctaccgtt tcctgcaaaa gctgttcata ttttttgttt
    30781 cctttatata aatcaccaat cgtccatgcc aacaggagaa ggacaagaac aatgattaca
    30841 taagcgatgt gatcagccaa tgctagttga gcggaagtgg cttgctcgcc gaagaaactt
    30901 tcgatctgtc ctggtaatcc tcgtaagttc aagtaagtta gactaatcac agagaaccag
    30961 ccaagaagtt ttacgatgaa tgaattttta aatcggctac ccatctccgc ttggctgttg
    31021 gtcatcatca gtaaaggaat catcgaaaac ggcaacgcaa aagccaagaa cacctgggat
    31081 tcattcatta agttattcaa agcggcatgt tcttcgatcg ttccttttgt acttgtaaac
    31141 aaaacacaaa tcaatacagg aatcacagaa agcaatcgtg tgaccagtct tcttagccaa
    31201 atcgggatac gcatatggat gaatccttcc atgatgactt gaccggtcaa cgtaccagta
    31261 atagtagagt tctggccaga agcaagaagt gctacagcaa ataaagtgga caagatacca
    31321 gattttgcta cagcaatcaa aataccattg ctcatagctg atggatcaga taaagcttca
    31381 tacagaccaa aaaaggaagg atcttggaca gaaccggttt tgaagacagc gacacccatg
    31441 atcaatagca gtgcattgac tagaaaagcc attgacaact ggatattaga atcccaagta
    31501 gaaaaacgta cagcttgtgc tacatcttct tcgttttggt gatcgatctt ccgagtttgt
    31561 gaaactgctg aatgaagata taagttatgc ggcatgactg ttgcaccaat aatccctaaa
    31621 gctccagtca gcggagtttc gccgccgatg accggtgagt ctgagaatgt atgactgtta
    31681 gggataaagc cagcaaataa actgtgccaa tctgggttag acaagccaac ttgatagaca
    31741 aaaacgaata aaatgacgaa aattaagcag acaaccagtg cttcgatctt acgaaacccg
    31801 acttttgtca gtagtaagag aacgaaaaca tctaatacag taataaagac ggcaatcacc
    31861 aatggaatat caaaaagtaa gtagagggcg attgctgcac caatgacttc tgcaatatct
    31921 gttgccataa tggctaattc agtcaagatc cataaaatga cacctaatgt cttacttgtg
    31981 cgtgctcgga ttgcctgtgc caaatccatt tggctcacga ttcctaattt tgctgccata
    32041 tactgtaaaa gcatagcaat cagactagac atcaaaataa ctgacatcaa taaatattgg
    32101 aaattttgtc ctccggtgat cgaagtagac cagttacccg ggtccatata cccaactgct
    32161 actaacgcac caggtccaga gtaagcaaga agtgttttaa aaaaactttt cccctttggt
    32221 acatcaactg ttccatttac ttcagctaaa gataaaccat ttgtatgttc aataagtcga
    32281 tgcttatgaa attgtttatt ggattgatta ttcaagaaaa aaccaccttt cctatttatc
    32341 tacgattaca ttctacacct caaaaagaaa aaattaaaga aaaagattcg gtacgcctaa
    32401 ttttttttca tggcgcgtct tgctagatga cgaaagaaat tttgttctcc tctaaaaaaa
    32461 ccactatttt ttgcatgaaa ctaaagaata cgcctttctt ttttcaataa actttagtaa
    32521 aataagtcat tgagagcaat tcggaaatag aaaggagaga tttatatgtt atcaacaggt
    32581 attgtcgtac ttattgctat tattgctttg ctaatcggcg cagtaggtgg ctttttccta
    32641 gcgcgtaaat atatgaaaga ctatttagaa aaaaatcctc ccgttaatga ggacatgttg
    32701 cgatcaatga tgatgtcaat ggggcaaaaa ccatctgaga agaaaattcg ccagatgatg
    32761 cagcaaatga agaaccaagg gaaaaaatag gttctgaccc aacgactttt tacgggctga
    32821 ttttttcagt ccgtttttta tttcgtaatt tgttagacta ttcagattat tcagcaaaaa
    32881 agacaagcac agacaatcct aggctcttga taaagacaaa ggaggagtaa aatgtcgatt
    32941 tttagaaaat taggctggtt ttttcgtcag gaaaagaaac attatttaat tggggttttc
    33001 tcgctattcg cagtggcctt ggtacagtta gttcctccaa aagtgattgg gatcatcatt
    33061 gatgagatcg ccgataaaaa tttatcaata aagatcattt tcgggtggat cttcctattg
    33121 attatcgctg cttttttaca atatcttttt cgttatatct ggcggacgaa tatttgggga
    33181 agtgcagctc gattggagaa agaacttcgg cagcagttat tcaaacattt tacagaaatg
    33241 gacaatctct tttaccaaaa atatcggact ggggatttga tggctcacgc aacgaatgat
    33301 ttgaatgcga tccagaatgt agcaggagct ggtatcttga cctttgcaga ttcagtgatt
    33361 acgggcggaa caacgatcat tgcgatgatt ttgtttattg attggcgctt aacactgatt
    33421 gcgctgattc ctctgccttt gctggccgtc acttcacgag tgctggggtc aaaactccat
    33481 gatgcttttc gtgattcgca agaagctttt tctagtatca atgataagac acaagaaagt
    33541 atcactggga tcaaagtcat taaaacattt ggacaagaaa aagaagatct agcggatttt
    33601 gaagaaaaga ttgaagatgc aattattaaa aacaaacgag tcaatttttt agactcctta
    33661 tttgatccat tcattacctt gattatcggt ctgtcttatg ttgcaacaat tattttaggt
    33721 ggaaagtatg tcttggaagg cacgatctcg atcgggcaga tggtctcatt tattagttat
    33781 atcggtatgc ttgtttggcc gatgtttgcg attggtcgat tgttcaacgt attggaaaga
    33841 ggaaatgctt cttatgatcg cgtcaatgaa cttttgcatg aaaagacgca tatcgtggaa
    33901 caactgggtg cagtgcagac cccggcaaaa ggcgagctga atgtagctat ttcctctttt
    33961 aattatccga aagaagaatc accagcatta gaacaaatca agtttgcgat ccaagaagga
    34021 gaaacattag gtattgttgg gaaaactggt gcgggaaaaa cgacgatcat caaattactg
    34081 atgcgtgaat acgatgatta tcaagggaaa atccaatttg gcggacgttc gatcaaagaa
    34141 tattcattga atgctttatt aggatcaatc ggctatgtgc ctcaggatca cttcttgttt
    34201 tcgatgacca ttcgagataa tatccgtttt tctaatccaa aactttcaga agaagaagta
    34261 gagcatgcgg cttctttagc atttatcaac aatgaaatca aggaattacc aaaaggttat
    34321 gataccttag ttggagaacg tggagtctct ttatctggcg gacagaaaca aaggatttcg
    34381 attgctcgag cattgatcaa aaatcctgaa ttattaatat tagatgatgc actctctgca
    34441 gtagatgcca aaacagaaga agctatcttg cataatttaa aagaaataag aaaagagaaa
    34501 acaactatca ttgcagctca ccgtttaagt agcgtgatgc atgcaaaaga aatcattgtt
    34561 gtcgatcaag ggaaaatcat tgaacgagga acccatcatg agctgcttgc actaggcggc
    34621 tggtatgaac aaatgtggga gaaacagcaa ttagaagcga agatagaagg aggcgaggcc
    34681 tgatggatga aaattatcaa tcggaatgga caaaatcgat tccacttaaa gaacagaaaa
    34741 agatcatcaa acgactgatg aagttcgcca aaccttttag aagaacgttc atcgttgcta
    34801 tcttatttgc gtttgctttg tctgtgatca atgtcctatt gccaaggatc atccaaactt
    34861 ttatggatga tcatctggct aaacagtcgg ctacgacaca agttattttg ttttttgctg
    34921 gtatgtactt atttggagtc atagtaaaaa gtatcatttg gtttttccaa tggtatttgt
    34981 attccatggc ctcattgaag acctatcagt atattagggt aaaattattt gaaaagctgc
    35041 atacactagg tatgcgttat tttgatcaga caccggcagg atcaaccgtt tctcgagtga
    35101 cgaatgacac agaaacgcta tttgaatttt ggtacgtctt tttgatggta ttgactggga
    35161 tctttgcagt cgtttcttca ttccttgcca tgtttcaaat caatggagaa attgccttgt
    35221 ggactttggt gtttttgccg attttaggaa tcgtcatctg gtattaccag aaattcagtt
    35281 ctcgtattta ccgaaatatg cgggaaaaat taagtcagct gaacacgaaa ttgaatgaat
    35341 cgatttccgg catgcgtatt atccagcagt ttcgccaaga agctcgttta gctaaagaat
    35401 ttgaacaagt gaacaatgag tatttagaca cgcgttatgc gatgatcaaa acgaattcga
    35461 ttctgttaag tccgatcatc aattttctat atgcattggc gattgcgttg gtgttgactt
    35521 tgtttgggat cgatgcgttg cattctcccg tagaagttgg actgatctat gcatttacga
    35581 cctatgtcca gggatttttt aatccaatgt ctcaaatgat ggactttttg agcatcttca
    35641 cagatgggat ggttgctggt agtcgtatct tgaagattat ggatacggaa gaatatgcac
    35701 cacaacaaaa ccctggagca gatcaaatga ttacttcagg taagattgag tttcgcaatg
    35761 taagcttttc ttatgatgga gagcacaatg tattaaacaa tatctctttt gttgctaatc
    35821 cgggagagac cgttgcatta gtgggacaca ccggaagcgg aaaaagctcg atcatcaacg
    35881 tattgatgcg gttttacgaa ttttatgaag gtcagatatt gattgatgat catgacattc
    35941 gagaatatcc aatggaagag ttacgtaaga aaatggggct ggtcttgcaa gatgctttct
    36001 tgttttatgg ggatatcgca ggaaatattc ggatgatgaa tccagctatc acagacgaac
    36061 agatacgcca tgcggctgag tttgttcaag caaacaaatt catcgatgaa ttaccaaaag
    36121 gatatcactc aaaagtgatc gaacatggtg ccagctattc tagtggacaa cgacagctga
    36181 tcacttttgc gcgaacaatc gtgactgatc ctaaaattct agtattggat gaagcaacag
    36241 caaatatcga tactgaaaca gaaggattga tccaagaagg attggcaaaa atgcgtcaag
    36301 gacggacaac gattgcgatt gcccatcggc tgtcaacgat tcgagatgca gaattgattt
    36361 tagttttaga caaaggtcat atagtagaac gcgggaatca tgaaacactt ctagcgcaaa
    36421 atggtttgta tgcagatatg tacaaactgc aaagcagtgg tatataaaag atgttgtgac
    36481 agtagaattt tgacctaagc actagagtga aaataaaaca tagcttctca tatttttcat
    36541 ctgtgagagt tagtgtcgta gtcaccagat ttaaaaaaag attcgacagc tagtcagaag
    36601 tgcctaatag ccactaagaa agcgttgaca aaagcatttg ctttcgataa tataaagaaa
    36661 acgatacaac ttggatagtg attgcctgat gcaagctaaa cctgatgagt ctcccatggt
    36721 ttcatggacg gctttttggg tttttctttt tgttttggat acagccctat acatcatgga
    36781 aaggagcaaa acatgctgat tgagaaagta ctgaataaca acgtcgtaat aaccagggat
    36841 gaaagaaatc aggaaatgat cgtgatggga aaaggtttgg cattcaaaaa gaaagtgggt
    36901 gacccgattt ttgactcatg catcgacaaa aaatttcagt tgacaaatga cgggctttca
    36961 caacagttcc aagaattggc acaagcgatt cctcttgagt acttagaaat ttcttgtgaa
    37021 atcattgaat atacaaaaga ggctctacaa gtccagttga atgattctgt gtatattgca
    37081 ttgacggatc atttgtatac ggctatcgaa cgtcacaaaa tgggaatatt tgtaccaaat
    37141 gtattgctgt atgatatcaa acaactattt cctaaagagt atgccatcgg gaaaaaaaca
    37201 gtgaagaaaa tctcggataa ataccacatc aagttatcag aagatgaagc cggatttatc
    37261 gcattgcatt tagtcaatgc acaaggcatt caaagccagc aagacaatac ctacctgttt
    37321 actataacaa tacaggatat tctaaatatc gtccgctatt actataagat tacatttgat
    37381 gaagattcag tccatttttt tcgattcgtc acacacttac gtttttttct gacacgcgtc
    37441 ctagatggag aaaaatcaga agtgggagaa gtttctgagg aattgtttca ggttattcaa
    37501 aaaaaatata aggatacgtt tggttgcgtt gagaaaatag cagacttttt agccgagacg
    37561 ttcaactacc agatgtccac ggacgaaaaa atgtatttaa cgatccatac tgcgcgttta
    37621 gtgcatgaga atcgttgtaa ataaaaaaag gaggataaat taacaagaac gtatgaaata
    37681 agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga atagaaatgg
    37741 gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag gaaaatatca
    37801 atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa gaaaaagcaa
    37861 atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg ggagggcagt
    37921 atcaagttgt cattgggaat catgtgccag tcgtgtttga agaggttata aaagcaggaa
    37981 accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat atgcttattg
    38041 atggaatatc gggctgtttc cagccattcc ttgctatcct agctgctggc gggatgattc
    38101 gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct ggcacatttg
    38161 ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt atcggtttaa
    38221 cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc gcagcattaa
    38281 tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca ctaacgacca
    38341 tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc attccctgga
    38401 ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg ttagctagcc
    38461 aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc ttcgttccat
    38521 tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca gtattcacat
    38581 tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg ccagttattt
    38641 atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc cattgggcaa
    38701 tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt atgacacctg
    38761 ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt aagcttagaa
    38821 atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg gggattacag
    38881 agccagctat ttatgggttt acattaccac gtaaaaaaat atttattttt acctgtgtcg
    38941 gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg aatcaaggtg
    39001 gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta acagatgtga
    39061 ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttacgctg acttttttct
    39121 tttggaagga tgaagcaggt gaaacagatg atatacagaa aaaatcaggt aaagaaattg
    39181 taaaaacacc gatacaagga caaatcgctc cactaaatgc agcaaaggat gcagcttttg
    39241 cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa gtacgtgccc
    39301 cttttgacgg aacagtcatg accctgttcc cgacgaaaca ggcaattgga atggtttctg
    39361 aaacagggct ggaattgctg attcatgtcg gattggatac tgtgcagttg gaagggaaat
    39421 attttgaaag cttagtgcag caaggataaa gagtgaaaca aggcgatctg ttgctacgct
    39481 tcgacaaaaa gaaaatcgaa aaggaaggat acagtttaga aacacctgtg atcgtcacca
    39541 attacacgga ttatttggat attttggaaa accaagggga gacggtcggg ccagacgatg
    39601 cacttatcac cgcactgacc tgaacggtta ggtgaagaat ggactaggct taactattct
    39661 ttttattcca cttctttatt ttcaaaattg aaaggctctt tcatgatggc tgagctgata
    39721 gggcgtttat cgaatttttg accgacagaa attagatgga ctaaattctg ttgataattt
    39781 tctgcaaaaa cacaactata taacacgtct agaagataac aaattgatgt attgatcgta
    39841 aaattaccaa tttttgaata cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc
    39901 attgcagata gcgttgaatc accgatactg gtcaaagcta aaattggaac atgattttgt
    39961 ttcaatattt ctgcggtacg aaggatcaca ttgttttcac ccgtataaga aatcagaatc
    40021 ccgcacatat cactcgtgca attgtacgct tcatagaatg attcaccaaa agtggtgaca
    40081 atcgtgacat ccattttgat gcgcttcatt ttcagcatga aatcttgaga aatcaaagta
    40141 ttcgagttac tggcgaaaat cacgattttt tttgcttgaa gcattagttg tttggctttt
    40201 tgcagttggt catgatgaat gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa
    40261 gccattttat ttgcgatcgt cataataccg tctgtggtgg aaaaaggaag attcgcatct
    40321 acgtcttgaa aattcccttc aagataggct gtttcagcta aaaaagcatt acgaaaactg
    40381 acccagccat caaaacccat tttttttgct acacggatga gtgtagaggg gtggacataa
    40441 catcgctcag caatcgcttg gatggtcatt tcgccaagtg ctggagaatc gcttaagaga
    40501 aactggatca cttcattttc tgaaggagaa aaggagattt ctttgatttt ttcacgtaat
    40561 aacatgtaga taccttcttc ttattttttt acgtaaatta taactttttt gaaaatgatt
    40621 tgtaaacgga tttatactat ataacttaaa atagacattt tttgacaaga tatttacaaa
    40681 aatccactag aaaacagtaa attttgtaaa tgttactgga aagaaacaga tttctattgt
    40741 ttgttttgtt tacaaaaaag ataatgaaag tgtaaaaaag taaaggagtg agaacatggc
    40801 aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg agttgatgga
    40861 aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg tagatgtaga
    40921 agaaggaaaa gaaaagcttg agatcgttag tcagatgtct cagcgtatgt gggatgcttc
    40981 tccgtatgaa gtcaagatct atgcaactct ggatcgggaa gaagcattga aagatgcgga
    41041 ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag acgagcggat
    41101 ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga tattcaaagc
    41161 ttttcgtacc gttccagtcg ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa
    41221 tgcttggctg atcaatttcg cgaacccaag tgggatggtg acagaagcag ttatcagata
    41281 tggcaaatgg gataaagtga ttggattatg caatgtgcca gtgatggcga tgatgacaga
    41341 accagcaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg gtctaaatca
    41401 tttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag aattgattga
    41461 tcgtttatac gatgaagaga atggattacc gaaaaatatc tttgatatcc cgtttttcaa
    41521 agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt actatcgtga
    41581 agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa gagcgcagca
    41641 agtgaaagaa acagaagcag agctatttga attatacaaa gatccagaat tagaccataa
    41701 acctgaacaa ttgcaaaaac gaggtggcgc ttattattca gacgctgcct gtgaaacgat
    41761 tgcttcaatc tatggaaata aagaaacaca gatcgtggta tctacaaaga atgatggagc
    41821 tgttcctgac ctgccggcag attgtgtagt agaagtcact gcctatgttg gcgcacaagg
    41881 agcgagaaat gtagcgtttg gttctcttcc tgctgctgaa agaggctggt tgcaagtaat
    41941 gaagaatatg gaattgctga cgatcgaagc agctgtgact ggtgattatg ggacagcttt
    42001 acaggcgttt acgatcaatc cacttatacg atcaggaaag acagcaaaac ggatcatgga
    42061 tgagttgttt attgcacacc agcagtattt accgcaattc aaggagacaa tcagccgatt
    42121 aaaagaagaa ggaatcgaag ttcaagatga actggcaaga gaattatcag gtaatacaca
    42181 tataatggag gaagtataat gggattcaga aaagattttt tatggggcgg tgctacagca
    42241 gccaatcaat gtgagggcgg ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt
    42301 gtgccaatcg gaaaagaccg ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt
    42361 gatgaggatc atttttatcc agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa
    42421 gatattgctt tgtttgcaga aatggggttc aagacttatc gaatgtccat cgcctggagt
    42481 cgtatttttc caatggggga tgagaaagag ccgaatgaag ctggcttgaa attttatgag
    42541 gatgccttta aagaatgcca aaagcacgga attgagccac ttgtgacgat tacgcatttt
    42601 gattgtccaa tgcatttgat caaagaatat ggtgcgtggc gttccagaga actcgttggt
    42661 ttttatgaaa atctgtgtcg agtcatcttc aatcgctata aaggacttgt caaatattgg
    42721 ctgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc agggctgtat
    42781 tttgaaaaag gagaaaatga agaacaaatc aaatatcaag cggcacatca tgagttggtt
    42841 gcaagtgcca tcgcaacgaa gattgctcat gaagtagatc cggagaacaa agtaggctgt
    42901 atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt ttgggcttct
    42961 agaaaatcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg agaatatccc
    43021 gcttatgcat tgaaagaaat ggagcgaaat ggcattcaga tcgaaatgga accaggggat
    43081 gccgcattgc tgaaagaaca tactgttgat tttatctctt tttcttatta tgcttcccga
    43141 gtttcgacta ccgacaacga actcttggaa cagactgcag gaaatatctt tgcttctgtc
    43201 aaaaatccat atctgcaagc aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga
    43261 ataacgatga atgatattta tgatcgttat caaaaaccat tgtttattgt agaaaacggc
    43321 ttaggtgcag tcgatacgcc agatgaaaat ggttatgtgg aagatgatta ccgaatcgat
    43381 tacctggcag cgcatatcca ggcgatgaag gatgcagtag aactagacgg agtcgatcta
    43441 ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtaccgg tgagatgaag
    43501 aaaaggtacg gattcattta tgtcgatcgt gataaccaag gaaatggcac attagcccgt
    43561 tcaaagaaaa aatcattcaa ctggtataaa aaagtaattg attcaaatgg agaaaatctt
    43621 tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt tttcgagtat
    43681 aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat tggctgtaaa
    43741 gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat cgttattggc
    43801 gcaggtcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt atccgtattg
    43861 atgatcgaac aaggtgcccc tggcggccaa atgaacaata cggcagaaat cgaaaattat
    43921 cctggattcg attcgatcat gggccccgat ctagcttata agatgtatga aagtgtctca
    43981 agatttggga cagaaaatgc ttatggaatc gttcaagata tcaaggatca cggcagccat
    44041 aaagaagtca tctgtgaaga taaaagttac gaaggaaaaa cagtcattat cgcaacagga
    44101 tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg aggcgtttct
    44161 tactgtgcag tctgtgatgg tgcgtttttc cgtaacaaac gtctagtagt catcgggggt
    44221 ggggactcag cagtagaaga agccatctat ttgacacaat ttgcttctga agtagtgatc
    44281 gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat
    44341 gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta
    44401 gtgacaggtg tgaaagcacg taatgtcaaa accggcaatg aatttgattt agcagcagac
    44461 ggagtattca tctatgttgg actggatcca ttaactgcac cttttagaga agcaggtttg
    44521 acgaacgaag ctggctggat tccaacagac aacgaaatga aaacgaatat gccaggcgtt
    44581 tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac
    44641 ggcggtattg ctggacaaca agtgtataaa tacttggagt ctttagaaga aacaacagca
    44701 taagtttaaa aagagtagaa gaacgggttc aattgttctt ttactctttt ttttcgcgtg
    44761 aaatttactg aatctgattt atgcgaacac gtccaacacc ttttgataaa agaatccttt
    44821 cttgatttaa gcgttttttg gtcctcatac tttcatatct aataagtgaa tgctatactt
    44881 attaagaacc atatttcaga aagaggtgtt attttcatgt cttgggaaca agtgtatgaa
    44941 caatgggtac atgaagaaaa tttagaagaa aacttaaaaa aacaattaaa tgatctgggt
    45001 gaagatcctg aaaaacttga agatgctttt tatgcaccgt tggaattcgg tactgcaggt
    45061 atgcggggaa tcttaggacc tgggatcaat cgaatgaaca tctatacggt aagacaagca
    45121 acagaaggac tagcacgttt catggataca caaggtgaag aaacaaaacg ccgaggtgtc
    45181 gctatcgctt atgactcacg ccatcagtca ccagaatttg caatggaagc agccaaaaca
    45241 ctagcacatc acaatatccc gtcttatgta ttcgaaagtc tgcgtccaac accagagctt
    45301 tcttttgcag ttcgtcattt gaagacgttt gcgggaatca tggtaaccgc atctcacaac
    45361 ccagcggctt acaatggata taaagtctac ggagaagatg gcggacaaat gcctcctgca
    45421 gacgcagatg cattgacgaa atatgtccgt gaagtcagca atccgctaaa agtcgaagta
    45481 ttatctgacg aagaagctaa acatagcggg ttgatcacga tcatcggaga agaagtggac
    45541 gctgcttatc tagaagaaat caaagcagtg acgattgatc gtgaattggt tgaaacaatg
    45601 gggaaagatt tgaaacttgt gtacacgcca cttcatggaa cagggaaaat gttgggcgaa
    45661 cgtgcgttga aacaagctgg attcgaacaa ttcgtactag ttcccgaaca agctgtggca
    45721 gatccagatt tcactacggt caaatcgcca aatccagaag aacattcagc ttttgaatat
    45781 gctatacgac ttggagaaaa agaaggagct gacttgctga ttgcaacaga tcctgatgca
    45841 gaccgtttag gcgcagcggt tcgtttgcca gatggttctt atgaagtatt gactgggaac
    45901 caaatcggag cattgtttgc tcaatatatt ttggaagcac atcaatcaag aggcacttta
    45961 ccagaaaacg cagcggtatt gaaatcaatc gtttccagcg aattgcctac agcaattgcg
    46021 gaacactacg gtgctaccat gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa
    46081 atccaacaat ttgaagaaga ccattcacat acttttatgt ttggattcga agaaagttat
    46141 ggttatttag ttaaaccttt tgttcgcgat aaagacgcga tccaagcatt gcttttatta
    46201 gcagaagtag cagcttacta taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg
    46261 atttttgaaa cctatggcta tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa
    46321 gaaggttctg cgaaaatctc tgctttgatg gaaaaattcc gtaaagaagc accgacagaa
    46381 tttgccggag caaaagttat tcaaacagaa gactttaaag aattgacgag aacagaccgt
    46441 gaaggaaacg tagaaaaact aacaacaccg ccatccgacg tattgaagta tacattggaa
    46501 gaaaatggct ggatcgctat ccgcccttca ggaactgagc cgaaaatcaa gttttatatt
    46561 ggtgtaaaag gaagctctac agaagacgca gccgaaaaag tagcctctta tgaagcgatt
    46621 atcaaaaaga tgacagaaga ataaaaaact tgagactgat ggaaaaagtg gtaaacttta
    46681 gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa aaatcaaaag
    46741 tagagaaagt cagtcaatta ttcaaaatgt tgagtgatcc cactcgtctt aaaattctgc
    46801 tatatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagtg gaaatggagc
    46861 agtctgctgt atcgcatcaa ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc
    46921 gcgagggaaa agcgattttg tatagtcttg atgattctca tgtactggat attttgaacc
    46981 agacactcaa acatgtggaa catcaataac gataggtgaa aatagataaa cactggagtg
    47041 tgacaacgaa gaaatttcgt tgtcacactc cagtgttttt taagaaaaag acaaaaatga
    47101 ttaatcgctt ttttctgttg tttcagttcc ataataacta tgatcttcta ggtctaaagc
    47161 attcaaaata agcgaagccg tttcttcaat agataaagat gccacattga tcactacaca
    47221 tcctagtttt tcataaagat cattcgcaaa atcgagttca gctctaattt tttcgatatc
    47281 ggaataagca gtatcagggt ttaatccgta agagcgcatc cgttctttac ggatattatt
    47341 taaaatatct gggttattcg ttagtcccac gattttttta ggatcgattt cccacagctg
    47401 tttgggaata tgtgcttggg gaatcaaagg taaattagca acttttaaat ttttatttgc
    47461 taaaaacaag ctcaaaggcg ttttggaagt tcgggatacg cctagcaaaa cgacatctgc
    47521 ttctaaaaaa ccacgaggat ctttcccatc atcgtatttt acagcaaatt ccattgcttt
    47581 gatccgtttg aaatagttct cattcaataa atggagggca ccaggttgcc gaattggctt
    47641 gataccagtt aaacgttcaa tttctgatat cggtggcgtc atcacatcga aactaaataa
    47701 ttgatgttct tcacaaaaag aacgtgttat ctcgatcagt tcttgattga ttaatgtatg
    47761 caggacaagt gctttttctt ctaatgcatc ttgcaatgcc tgccttaatg ttttttcatc
    47821 tgtcacgaac gttctgcgga ataggttgaa ctcaacggta ggatactgag ccatagtagc
    47881 ttgcgctagt tttgtcgcag tttctccagc agagtcggag atgacaaaaa aagtaacgat
    47941 ttctgtagaa tcggaagaca tggataaaac ctcacttgca ttttttttat gtttattata
    48001 ccatactcta gaatgcgtac ttcgtaatca ttttgatttg gaggaataag tcatgaaaaa
    48061 acagacattg atcgaacaat cattagaaac attgaaaaat aacggtttta aatatactaa
    48121 aaaaagagaa gcattgttaa cttatttagt gaagcgtaac cgctacgtat ctgctaaaga
    48181 agtttttgac tatatgaatc aggaattcaa aggggtaagt tatgatacga tttaccggaa
    48241 tctccatgat tttgcagaga tcgatttact ggaagaaacc gatttaaatg gtgaaatgaa
    48301 gtttcgtttt cattgctgtc agggggaaat cggccatcac catcatttta tctgtacagt
    48361 gtgcggaaca acgaaagaaa tccatatgtg tccaatgaat tatttcgaag aacagctagg
    48421 tggatgcacg atcgaagggc atagattcga gattttcgga agatgtgaaa attgtcaata
    48481 aggatttaaa taaaataaca tttgataatt taaaaaaaaa gcaagatttc cttgacggtc
    48541 aaaacagcat tagatataat atttacagga cagtcttgtt ttttgagata tatttcaata
    48601 acaattacgg ttacttttta agctcggagg gagggaatta acatgtcaaa aacagtcgtt
    48661 cgcaaaaacg aatcacttga cgatgctctt cgtcgcttca aacgttccgt gtcaaaagcg
    48721 ggtactttac aagaatctcg taaacgtgaa ttctacgaaa aaccaagtgt aagacgcaag
    48781 aaaaaatctg aagcagctag aaaacgcaaa aaattctagt tctgtgatga attggagttg
    48841 gtagtgtgtc actactaagt agattaaacg atgacatgaa agcagcgatg aaagcgaaag
    48901 ataaggaatc tttacaagtc atccgaatga tcaagtcatc tatacaaaac gagcagatta
    48961 aagaaggtca cgacttgaca gaagaagaag aactaacggt gttgtctcgc gaaatgaaac
    49021 agcgacgtga ttctttgcat gagtttgaag aagcaggacg tgacgattta gctgaaaaag
    49081 taaaaagtga aattgtaata gtcgaaaaat atatgccgga acaactttcc gacgaagaaa
    49141 ttcgacaact tgttcaagaa gcaatcactc aaactggtgc ttcatcaatg aaagaatttg
    49201 gcaaagttat gggtgcaatt atgcctaaag tcaaaggaaa agcagatgga aaccaagtga
    49261 atgcgattgt gaaagaacta ttacaatctt aattttatca tttaaactgc ggacagtgat
    49321 tgtccgcagt tttttgtgtt cgtaaaaaaa gaactaaaaa acagctagaa gaaagaataa
    49381 gattctttct tctagctgtt tttttcctgt ttaatatctc ttatgttaaa ttgtgtttga
    49441 tttggttatt cttctggtag ttcatttgct tcttgtttat accctgatag gcgataggcg
    49501 taataaaaga gtaagctgcc agcgtaacca agtaacgttg ggaccatcca gccaagtcct
    49561 aatgcagcaa aaggaacgtg atgatgataa aatcctagta agcttgctac gacagtttgc
    49621 tgactcacta atgcaggcga atttgccaac atatccaata cggctggaac ggcagaaaag
    49681 aacatcgtta cttgatagac aaatggactc ttttggaaga attttgctgt taaggaaagc
    49741 aagattaacg caagtgacaa tggatacaaa agcatcaata ctggtaaaga ccattggatg
    49801 atattatcca atccggcatt cgcaacgaca aaagagacaa aagtcgttaa ccgcagccat
    49861 gtcatataac tgactttagg gaacagttta tggaaatctt gtgcaaaaga aacaacgagt
    49921 cccatagcgg tagtgaagac acctaatgtg accattaccc ctaagaagct agcaccgaag
    49981 ttacctaaat aaaaattagt gatctgagaa agtgcagttc cgccattttc tgataaaggc
    50041 atcttattca aactcatcgc accgagaagg accagaccaa aataaatcat gacttctaac
    50101 gcaacgctca ggctaccagc ttttgctgtt aattctgtga cttttttatc tttaaaacca
    50161 agatcacgaa ctgcatggac aaaagtgaca cttaaagcaa gtaaagctac tgcatcaacc
    50221 gtgttgtatc cttctaaaac gccatttgtc aaagcgttcg tctgataaga atctgcagca
    50281 gattggttta gattccccat gggttggaat aaagaaacga caaaaatgat agctaataat
    50341 gcaagaaaca aggagtttaa atactttccg acatatttga ccaaacgtgt tggatgtaca
    50401 gtaaggaaat aagcaagtaa aaagaaaaga gcagagaata aaaacatacc taatgaatca
    50461 tatttttcag gcaaaaaggg tttagcagcc atttcaaaag cagttgctgc tgtccgcggt
    50521 gtaccaaaga atggtccgat cgttaaatga acaaggatca agaacaaggc agcataccat
    50581 tttccgacag gtttagctaa gtcatacaga ccatcacttt ttgtcactac tacagctaag
    50641 atagctaata atgggaaaag agcgcctgag atggcaaatc ctacaccagc gctgatccag
    50701 tttgatccag ccatttgacc taagtggaca ggaaagatca gattacctga cccgaagaac
    50761 atcccgaaga taagtgaact gaccatcaat aattgcttcc atgaataagt ttttgtttct
    50821 tccataaatt tttcctccaa taattatcat tttgagtaac aaaataaacg ttgcagaaac
    50881 tagaaatgcc actaatatca gattccatga aaaccaccac cttaaaatat aaaaaaaagc
    50941 ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt tttcgcttaa
    51001 aaaagcttct tcattcacgg acgcttttcg ataaagttat ccgctagcct gataatggag
    51061 gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt ctattcgatt
    51121 tattgtcatt tctcacttac atgactcact gggaataaat cgctgaatag cctactgttt
    51181 tcctcgacgc tttgctgtat atttatttgt tgacgttata atacacctgt tttttttgaa
    51241 atgcaagaat atttcaaata ttttgttttc tttttttgaa acaagaaaaa tacccagaga
    51301 atacttgcta agttagacgg aatacttgtc ctctatctta tgcatagctt acatgatcag
    51361 ctttttttat tcaaacgaga cctttagaga atggaagcag ctatttttta acgtttcttg
    51421 gtacttatgg ttaagcaact cttttagaac cttttcttat ttgctatata gtttcttctg
    51481 agtatggtaa aataggaaag aatgaatcga aaaaaagggg atgtctattt ttgacggaag
    51541 agtctagttc tttagagatt agattgacgg ctgctgatga tgtcagcatg cttttaggtg
    51601 cacacgacaa gcatataaaa ctgattgaag aaacaacaga aacaaccatc catacacgtg
    51661 gagaaatcat tcagatcatt ggcgaacaat cccagatttc cttggctgcc tcagtcattc
    51721 ggacattgca ggaattgatc aaacgaggaa tccatgtaag tacaccagat gtcgtgacgg
    51781 cactgaaaat ggggcaaaaa ggcacattag attattttgt tgaaatgtat gaggaagaga
    51841 tcgtcaaaga ccgtaatgga aaaccaatac gggtaaaaaa cagcggccaa aagaaatatg
    51901 tcgaatcggt agccaaacat gatatcgtat ttggcgtagg accagccggg acaggaaaaa
    51961 cttttcttgc agtagtatta gcaatcgctg ccttgaaaaa aggtgaagtg caaaaaatca
    52021 ttttgacgcg gcctgctgtg gaagcagggg aaaatctagg ctttcttccc ggggatctaa
    52081 aagaaaaagt agatccttat ctgcgtcctg tttatgatgc tttataccaa atttttggat
    52141 tagatcatac gaaccgattg atggaacgag gcgtgatcga aattgctcct ttagcttaca
    52201 tgcgggggcg gacattggat gatgcattcg ttattttgga tgaagcacag aacacgacag
    52261 ttgctcagat gaaaatgttc ctgacacgtc ttggttatca atcaaaaatg attgtcaatg
    52321 gagatacgag tcagattgat ctgcctcgag ggacaacaag tggtcttgtt catgcagaac
    52381 gaacattaaa gcaaatcaag aaaatcgatt ttgttaattt tgaagcaagt gacgttgttc
    52441 ggcaccctgt agttgccgaa atcatcaaag cttatgaaaa ggcagatctc catcaagaat
    52501 aagctttgct taggaggata agcacatgtt gaataaaaca ctgcagaaac tacaagaaaa
    52561 acttggaaag gcagcattgc ctttcctgct gttcttgttt tttatcatta tctgcggcgt
    52621 tacatttagt agtgtcaaac aaaaggccaa tgatttcaga gaaggccaag tagcagaaga
    52681 aagtatccgt gcgaacaaaa cgatcgagaa cacggaagaa accgaacaaa aaagaaaact
    52741 ggcagcagaa gcagtcactc cagaatacac ttaccaaaaa gatcttgcag atgaccagaa
    52801 caatcggatc aagcaactat ttgaattgat cgataaaacg aatgatgcca tcaacaaatc
    52861 ttatgacgaa aagattgata aggcaaagga agatgaaaat atcccaaagc ctactacaga
    52921 agagcggatc gccgcgttga agaaaaattt tgaaaatgtg aatgcagaaa atgttgcgtt
    52981 ttatcaaaaa ctaccgaaca atttttattc gacgatattt gaaatgactg aaactgagat
    53041 ccatactgtg agggatgaga gtctgaagct cattgacgaa cagatgagca aacaagttcg
    53101 tgaaagtgaa ttggaagctt ttaaacaaga agcagaagag caaatccaat atttgaatgt
    53161 cacaccagca caacaacaaa tgattcgcta cttagtggat caaggtatcg tagtgaatga
    53221 cgtattgaat gagaaaaaga cagaagaatt gaaacaatcc gcacgagaag ctgtacaacc
    53281 agtgatgatc ttccaaggag aaatcattgt tcgtgaaggt aatcaaatcg atgcttcagc
    53341 tatgaaaaaa ttggaattac taggactgac taatcaaaca acttcgattt ttccgctaat
    53401 tgccatggtt ttagctgttt tgcttcaaat cggcgtactg atttataatt cacttcaatt
    53461 ccaaaatgca ggagaaagaa caaaatacgt gcttttctat gtgacagcta tgtcagtcag
    53521 catagtgctg atgaaatttt tccaattatt ccaaacggaa caagctactt atatcccgct
    53581 gttttatccc gctgcttttg caccacttgt tttaagcttt ttcttgaacc gaagagcagg
    53641 gatcatggct ggtattttcc aagcagtatc tgcacttttc attttttatg attcgattgg
    53701 cacgaacttt ctaacgatta tcttgatgtc ttatgttttc tctgggctca tggcaacgat
    53761 tgtcagacgc aaacggatct cagaacaagg attatctgct gccatgtggg tcattatttt
    53821 tcctgtgatg atggatgtca ttttgatcgt ctatcaagga atgagctttg gtgatgcaac
    53881 gacttggtta atgcttgtct gcgggttcac tggtgcactc ttctcattct tgctgacgat
    53941 cggtctacac ccatatattg aattgatggt caatgatgac agtgtaattg tattaaatga
    54001 actcagcaat ccaaatcatc ctttattgaa acagttgctg gaagaagcgc caggtactta
    54061 tcatcatagt atgatggtag ctaatctaag tgctaacgct gtagcagaaa tcggcggacg
    54121 ttctttgcta actcgagtag cttgctatta tcatgatatc ggcaagatca aacatgctag
    54181 ctttttcgtt gagaacttgc cttcaggagc ggaaaacccg cacaatttct tattgccaga
    54241 agacagtaaa catattatct ttggacatgt gacagatgga gccaaaatat tggaagatta
    54301 taatatgccg caaatggtga tcgatatttg caaacagcat catggaacca cgctaatgcg
    54361 ttatttctac atcaaagcga aagaacgcaa tccggaagta acagaagagc aattccgtta
    54421 tcctggacca aaaccgcaaa gtcgtgaagc agggatcgtc aatattgctg acagttgtga
    54481 ggctgcagta cgggcaatgg atcatccgac gagcgaaaaa atcgaaagtt ttgtccataa
    54541 tcttatcgaa gagcgtattt ccgatggtca attagacgat agtggactga ccttgaaaga
    54601 aatccgaaaa gtggaaaaat cattgatcag cggtttaagt agcaccttcc attctcggat
    54661 caaatatcct aaaatgaaaa cagaagttga gaagataaag gaagaacaag aagaatagaa
    54721 agaagcgatg tataatggat attaccttta tggatgaaac aaaccaagtc tcagaagaga
    54781 aaatgaaaga aatcgatgac ttgctgcaat ttgcagcatc ttatttaaag ctgccagagg
    54841 aaacagaaat gtctgttact ttcatggaca atgcggcaat ccaagtaatc aatcgtgatt
    54901 accgtggaaa agatgcaccg acagacgtca tcagttttgc tctggaagaa gaaggcgaag
    54961 acgaaatccc agtgattttt gaagatggag agaatccatt gcctcgtgta ctaggagaca
    55021 tcatgatttc tattgaacgt gctgctgaac aagcagaagc ttacggccat tcatatgatc
    55081 gggaacttgg ctttttagcg gtacatggat tcttgcatat caacgggtat gaccatatga
    55141 caccagaaga tgagaaagaa atgtttggtt tacagaaaga gatcttggat gcctatggac
    55201 tcaaaagata aaaacgtaga gaaaaacaaa catttcctga tgtctgtgga atttgctgtc
    55261 caaggagtca aaaccgtttt tgaggaagaa cgcaatatga ggaaacatgt tgcgttcggt
    55321 atccttgctt tatttatggg cgtgattttc cagcttgatc ggatagagtg gttatggctg
    55381 cttttagctg tttttctcgt ttggatagta gagatcatca atactgtttt tgaaaatgta
    55441 gtggacatgt ttacggattt tcattttcat ccgattggga aaaaaatcaa agatatggca
    55501 gctggagcag ttttagtgac cgcttgtttt gctgtcatca ttgggttgat tttatttgtc
    55561 cctaaaatat atcatttgtt gtttaattaa aaaggagaac gtatatgcca gaacataaat
    55621 cagggtttgt cgcaatcgtc ggccgcccga atgtaggaaa atccacatta ttgaaccgaa
    55681 tcgtgggtca aaaaattgcc attatgagtg acaaggcaca gacaactaga aataaaatcc
    55741 aaggagtata tactacacca gaagctcagc tgatttttat cgatacacca ggaatccata
    55801 aaccaaagca tcgtctagga gattttatgg tagaagcagc ttacagtgcg ttgaaagaag
    55861 tggatgcagt cttatttatg atcagtgctg atcaaaaacg cggccgggga gacgatttta
    55921 ttattgaacg tctaaaaaac gtgcattctc cagtttatct gattatcaat aaaatcgata
    55981 agatccatcc agacgagttg ctgggaatca tcgaagatta ttcgacgcag atgccttttg
    56041 cacaagtgat tccgatctct gctacagaag gaaacaatgt cgaacgctta atggatgtat
    56101 tagtcagtga aatgccagag ggcccacaat attttccaga tgatcaagtg actgatcatc
    56161 ctgaatactt tatcgtttct gaattgatcc gtgagaaagt cttgttttta acacgagacg
    56221 aagtgcctca ttctgtcgca gtcgttgttg attcgatgaa gcgaaacgag aatgataaaa
    56281 tccatatcca ggccacgatc atcgtcgaaa gagatagcca aaaaggaatc attatcggca
    56341 aaggcggaaa aatgctgaag caagtaggaa cgaaagcaag aaaggatatc gaaaatcttt
    56401 taggagacaa agtatttcta gaactttggg tcaaagtcca aaaagactgg cgagacaaaa
    56461 aagtctatct gcaagacttt ggctaccgaa aagacgaata ctgatattct gaacttctta
    56521 taggttaagt atagaaataa actggataaa aatccgcacg ttgagaaaaa cggcggaaga
    56581 tcgaaacata aataatctgg atcggcagaa tctgcttttc cagatttatt ttttaggagg
    56641 gagaatatga gccagcttgc agaatcgaaa ggcttgattt tatctagccg taattacaaa
    56701 gaaaaagata aattagtcaa aatctttacg gaatcttctg gcaaaatgat gttttatatc
    56761 aaaggaatcc atcggcaaaa tcagccatta gctccggctt tgctcccctt taccgaagca
    56821 gtctatatcg gtaattttcg aagtgaaggt ttgtcgtttt taagcagtgt caaagacgtc
    56881 catgcgtttc ggaacatcca agaagatatc tttttgagcg gttatggtac ctatttgcta
    56941 aatctagtag atgcagcaat cgaagatcat caatatgacc cgcatttatt ccaatttacg
    57001 caacaagctc tgcagcggat ggatcaagga gatgatgcag aaatcatcac gaatatcttt
    57061 gaagttcaaa tactgcaacg cttcggaatc gcgccaattt ggacgcactg tgtcgtatgc
    57121 ggggaaacaa aaggaaaatt tgactattct tccaaatctg gcggtgtgat atgcgagaaa
    57181 cactggccaa tggacgaaca ccgttatcat gcagatcctc gcgctgttta tttcgttcga
    57241 atgttttcgg ctatttctta tgataagatc agtggaatcc acttgaaaga agaaacgaag
    57301 caggcaatcc gtgacctcat tgaccagttg tatgatgaat atgtcggtat tcatctaaaa
    57361 agcaaaaagt tcattgatca aatgaagtct tgggcacacg tattaaagac gccagagaaa
    57421 aatatgcctg aaaaagagat acctgaaaaa gaagaataaa tgaaagatac aacaaaaaaa
    57481 tcaaaactat ttgacaaata cagggacttc cgtctattat taaccgtaga ttaaatagat
    57541 cgttcactga gaggatctat tatataaaca agaaaactgc aaaatgaata cgtacattga
    57601 ccaagatcag taaaaaacac gtggtttagc gagttcggga gagtggaagc cggacccaaa
    57661 cgatttttga agaggcactt gcgagtacga taaatgaagc tgccgaatct ttattcggaa
    57721 gtagggtgga accgcgaaat tattcgtccc tatgtctgta atcagacata gggacttttt
    57781 gtcgttttct ttcctgtatt cttattgaca gcaacgctta aaaattgaaa ggggcaaaac
    57841 agatgagtaa aaaattgacc gtccaagata tgattttaac attacaaaaa ttttggtcat
    57901 caaacggctg catgctgatg caagcttatg acacagaaaa aggagcaggt acgatgagcc
    57961 catatacatt cttacgggca atcggaccgg aaccatggaa tgctgcttat gtagagccat
    58021 cccgccgacc agctgacggg cgttatggag aaaacccaaa ccgtttgtat cagcatcatc
    58081 aatttcaagt cgtaatgaaa ccttcacctg aaaatatcca agagctttat ttagaaagct
    58141 tgcgtctttt aggaattgat ccattagaac acgatattcg ttttgttgaa gacaactggg
    58201 agaacccatc gatggggtgt gccggtgtgg gttgggaagt atggcttgat gggatggaga
    58261 tcacacaatt cacgtacttc cagcaagtcg gaggactacc ttgtaaacca gttacttctg
    58321 agatcactta tggactagaa cgtcttgctt catatattca agaagttgaa agtgtctatg
    58381 atctggaatg ggctgatggt gtgaaatatg gcgagatctt caaacaacca gaatatgaac
    58441 attccaaata ttcttttgaa gtaagtgacc aagacttgct actaagcaac tttgaccgtt
    58501 ttgaaaaaga agctaaacgc tgtatcgatg aaaatcttgt tcatccagcg tatgattata
    58561 ttttgaaatg cagccataca ttcaatttat tagatgctcg cggtgcagta tccgtaacag
    58621 aacgagcagg ttatctttcc cgtatccgca acatggctcg tgctgtagcc aaaatctttg
    58681 tggcagaacg cgaaaaatta ggattccctt tattgaataa aaacacagca aatccagaaa
    58741 acatgaagga ggctgaataa gatggcaaaa gacttattac ttgaaatcgg gctggaagaa
    58801 atgcctgctc atgttgtcac accaagcatg aaacaattag aagaaaaaac agcaaaattt
    58861 ttagatgaaa accaattgac ttatgatacg atcgaaacgt tttctacacc gcgccgctta
    58921 gcaatcaaag taacaaacat tccagaacgt caagcagaca gtgaagaaga agtcaaagga
    58981 ccagccaaaa aaattgcttt ggacgcagat ggaaactgga cgaaagcagc tgaaggattt
    59041 gtccgcggac aaggtctgac gacagatgct atcactttta gagaattaaa aggcgtggaa
    59101 tatgtctatg taacgaaata cactcacggc aaagaagcga aagaagtatt gacaggttta
    59161 gcagatgtca ttaaaagcct cactttccca gtaacgatgc attgggcaga atacgatttt
    59221 gaatatatcc gccctattca ctggatcgtg gcattgttag atgacgaagt aatcccgttt
    59281 gagatcttag atgtcaaaac aggcagagcc tctcgaggac atcgtttctt aggcgaagat
    59341 gtgacatttg cgcatgcaaa agaatatgaa gaaaaattga atgaacaatt cgttattgct
    59401 tcaccgcaaa aaagacaaga aatgatcgtt acacaagcaa atgaattagc cgaaaaaaat
    59461 cactgggtta ttgatctaga cgaagattta ctagaagaag tcaacaattt agtcgaatat
    59521 ccaactgtct tttctggcga cttcgaagaa aaatatcttt ctgttcctga agaagtacta
    59581 gtcacttcta tgaaagaaca tcaacgttat ttcgaggtac ggaacgatca aggtatgctg
    59641 cttcctcatt ttatttctgc tcgtaatggt gatcgggtaa aattggaaaa tgtggttcga
    59701 ggcaatcaaa aagtattgac ggctcgttta gaagatgcgg aattctttta taatgaagac
    59761 aagaaagctt caatcgacca atacgttgaa aaactaaaaa ctgttacttt ccatgaaaaa
    59821 atcggtagta tctatgagaa aatgcagcgg gtacaagtca ttgcccgtat cattggcgaa
    59881 caagtaggac tatctgagga agaattgatg gatctgcaac gagctgctga aatctataaa
    59941 tttgatctag taaccaacat ggtaggggaa tttcctgaac tacaagggat catgggcgaa
    60001 aaatatgctc tgctgaaagg cgagaaacct gctgtagcaa cagcgattcg tgagcattac
    60061 atgcctatat ccagtgaagg agagctacct cagtctacag taggttctgt tttagcgatt
    60121 gcagataaat tagatagcat tttttctttc tttgcagttg ggatgattcc aagcgggtca
    60181 aacgatcctt atgccttacg tcgccaagca tacggtgtcg ttcggatcat cgaatcgaaa
    60241 aaatgggcct ttcctttgtc tgttctgcaa gaaacgatta gtgatgttat cagtaaagat
    60301 acggatcgtt tcggcattgg tttatcagaa gggcaacaac aggtgatcga ctttatcaaa
    60361 ggccgtctgc gccaattatt gacaacgaag aatattcgtc atgatgtcat cgaagctgtt
    60421 ttgaatgcag aacaaaaaga tctgacaaaa gtatttgcag cagcacaatt gttcaagcag
    60481 catcttgcag atgaagactt caaaccatcg atggaagctt tgacacgtgt cgtgaactta
    60541 gcgaaaaaag cggagcttga aaaacaatca gaagtagacc ctgaactgtt cgaaaatgaa
    60601 gcggagaaag aactgcacaa agcagtagag gcaatgaaaa aaagctttgc tgaccaaacg
    60661 atcaacgaaa attatgatca gcttgtaagc cttcgcccac tgatcgaaaa ttattttgat
    60721 cagacgatgg tcatggcaga agaaccagca gtacgtaaga accgattaac gcaattaagt
    60781 caaattgctg ccatggcttt gtcgctagca agtttagatc aaatcatcac aaaataattt
    60841 ctaatatgta aataaaccag agaataggca caaaa
//
LOCUS       DARBEG010000007        60538 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000007 DARBEG010000000
VERSION     DARBEG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.007"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(12..1535)
                     /locus_tag="SCK20_000428"
     CDS             complement(12..1535)
                     /locus_tag="SCK20_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1144523.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="SCK20_000429"
     CDS             1787..2968
                     /locus_tag="SCK20_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1144524.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCK20_000430"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCK20_000430"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG1144525.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="SCK20_000431"
     CDS             complement(5053..7764)
                     /locus_tag="SCK20_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144526.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="SCK20_000432"
     CDS             complement(7774..8475)
                     /locus_tag="SCK20_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144527.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="SCK20_000433"
     CDS             8661..9260
                     /locus_tag="SCK20_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1144528.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="SCK20_000434"
     CDS             9341..10354
                     /locus_tag="SCK20_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1144529.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="SCK20_000435"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="SCK20_000435"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG1144530.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="SCK20_000436"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="SCK20_000436"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG1144531.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="SCK20_000437"
     CDS             11802..13175
                     /locus_tag="SCK20_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1144532.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="SCK20_000438"
     CDS             13532..15676
                     /locus_tag="SCK20_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1144533.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="SCK20_000439"
     CDS             complement(15728..16768)
                     /locus_tag="SCK20_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1144534.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="SCK20_000440"
     CDS             complement(17045..18847)
                     /locus_tag="SCK20_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144535.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="SCK20_000441"
     CDS             complement(18861..19613)
                     /locus_tag="SCK20_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144536.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="SCK20_000442"
     CDS             complement(19622..19984)
                     /locus_tag="SCK20_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1144537.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="SCK20_000443"
     CDS             complement(20160..21032)
                     /locus_tag="SCK20_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1144538.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="SCK20_000444"
     CDS             complement(21058..21570)
                     /locus_tag="SCK20_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG1144539.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="SCK20_000445"
     CDS             complement(21708..22499)
                     /locus_tag="SCK20_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG1144540.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="SCK20_000446"
     CDS             complement(22554..23162)
                     /locus_tag="SCK20_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG1144541.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="SCK20_000447"
     CDS             complement(23155..23472)
                     /locus_tag="SCK20_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1144542.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="SCK20_000448"
     CDS             complement(23628..24356)
                     /locus_tag="SCK20_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG1144543.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="SCK20_000449"
     CDS             24515..25396
                     /locus_tag="SCK20_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1144544.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="SCK20_000450"
     CDS             complement(25633..26676)
                     /locus_tag="SCK20_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1144545.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="SCK20_000451"
     CDS             complement(26679..27347)
                     /locus_tag="SCK20_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1144546.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="SCK20_000452"
     CDS             27489..29825
                     /locus_tag="SCK20_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1144547.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="SCK20_000453"
     CDS             complement(29940..30251)
                     /locus_tag="SCK20_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144548.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="SCK20_000454"
     CDS             30432..31907
                     /locus_tag="SCK20_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG1144549.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRNIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="SCK20_000455"
     CDS             31954..32538
                     /locus_tag="SCK20_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG1144550.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="SCK20_000456"
     CDS             32540..32959
                     /locus_tag="SCK20_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG1144551.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="SCK20_000457"
     CDS             complement(33240..33524)
                     /locus_tag="SCK20_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG1144552.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="SCK20_000458"
     CDS             complement(33876..34586)
                     /locus_tag="SCK20_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG1144553.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="SCK20_000459"
     CDS             complement(34583..36055)
                     /locus_tag="SCK20_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1144554.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="SCK20_000460"
     CDS             36193..36666
                     /locus_tag="SCK20_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1144555.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="SCK20_000461"
     CDS             complement(36819..37370)
                     /locus_tag="SCK20_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1144556.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="SCK20_000462"
     CDS             37475..38005
                     /locus_tag="SCK20_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG1144557.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="SCK20_000463"
     CDS             complement(38488..38931)
                     /locus_tag="SCK20_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1144558.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="SCK20_000464"
     CDS             complement(38947..40107)
                     /locus_tag="SCK20_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1144559.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="SCK20_000465"
     CDS             40614..42314
                     /locus_tag="SCK20_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144560.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="SCK20_000466"
     CDS             complement(42416..43249)
                     /locus_tag="SCK20_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG1144561.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="SCK20_000467"
     CDS             complement(43553..45718)
                     /locus_tag="SCK20_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1144562.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="SCK20_000468"
     CDS             45978..48272
                     /locus_tag="SCK20_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1144563.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="SCK20_000469"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="SCK20_000469"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1144564.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="SCK20_000470"
     CDS             48923..49930
                     /locus_tag="SCK20_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1144565.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="SCK20_000471"
     CDS             49952..50971
                     /locus_tag="SCK20_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1144566.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCK20_000472"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCK20_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1144567.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="SCK20_000473"
     CDS             complement(52729..54150)
                     /locus_tag="SCK20_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1144568.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCK20_000474"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCK20_000474"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1144569.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="SCK20_000475"
     CDS             complement(56561..57817)
                     /locus_tag="SCK20_000475"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG1144570.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="SCK20_000476"
     CDS             58397..59698
                     /locus_tag="SCK20_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1144571.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="SCK20_000477"
     CDS             complement(59767..60366)
                     /locus_tag="SCK20_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1144572.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        18278 a        12374 c        10842 g        19044 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgcaacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtt
//
LOCUS       DARBEG010000008        56576 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000008 DARBEG010000000
VERSION     DARBEG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.008"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            279..665
                     /locus_tag="SCK20_000478"
     CDS             279..665
                     /locus_tag="SCK20_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1144573.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            759..950
                     /locus_tag="SCK20_000479"
     CDS             759..950
                     /locus_tag="SCK20_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144574.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1464..1796
                     /locus_tag="SCK20_000480"
     CDS             1464..1796
                     /locus_tag="SCK20_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1144575.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2092..2247
                     /locus_tag="SCK20_000481"
     CDS             2092..2247
                     /locus_tag="SCK20_000481"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1144576.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2523..3863
                     /locus_tag="SCK20_000482"
                     /pseudo
     CDS             2523..3863
                     /locus_tag="SCK20_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4104..5720)
                     /locus_tag="SCK20_000483"
     CDS             complement(4104..5720)
                     /locus_tag="SCK20_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1144577.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            5838..6056
                     /locus_tag="SCK20_000484"
     CDS             5838..6056
                     /locus_tag="SCK20_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144578.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6251..6712
                     /locus_tag="SCK20_000485"
     CDS             6251..6712
                     /locus_tag="SCK20_000485"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1144579.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6905..7144)
                     /locus_tag="SCK20_000486"
     CDS             complement(6905..7144)
                     /locus_tag="SCK20_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144580.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7168..8691)
                     /locus_tag="SCK20_000487"
     CDS             complement(7168..8691)
                     /locus_tag="SCK20_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1144581.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8709..8831)
                     /locus_tag="SCK20_000488"
     CDS             complement(8709..8831)
                     /locus_tag="SCK20_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1144582.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(8861..9844)
                     /locus_tag="SCK20_000489"
     CDS             complement(8861..9844)
                     /locus_tag="SCK20_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1144583.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(9868..10017)
                     /gene="rpmG"
                     /locus_tag="SCK20_000490"
     CDS             complement(9868..10017)
                     /gene="rpmG"
                     /locus_tag="SCK20_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1144584.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10038..10415)
                     /locus_tag="SCK20_000491"
     CDS             complement(10038..10415)
                     /locus_tag="SCK20_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144585.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10447..10626)
                     /gene="rpmF"
                     /locus_tag="SCK20_000492"
     CDS             complement(10447..10626)
                     /gene="rpmF"
                     /locus_tag="SCK20_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1144586.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10641..10910)
                     /gene="rpsN"
                     /locus_tag="SCK20_000493"
     CDS             complement(10641..10910)
                     /gene="rpsN"
                     /locus_tag="SCK20_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1144587.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11433..13199)
                     /locus_tag="SCK20_000494"
     CDS             complement(11433..13199)
                     /locus_tag="SCK20_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144588.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13159..14912)
                     /locus_tag="SCK20_000495"
                     /pseudo
     CDS             complement(13159..14912)
                     /locus_tag="SCK20_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(15022..15591)
                     /locus_tag="SCK20_000496"
     CDS             complement(15022..15591)
                     /locus_tag="SCK20_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG1144589.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(15609..17024)
                     /locus_tag="SCK20_000497"
     CDS             complement(15609..17024)
                     /locus_tag="SCK20_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1144590.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17035..17703)
                     /locus_tag="SCK20_000498"
     CDS             complement(17035..17703)
                     /locus_tag="SCK20_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1144591.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(17833..18417)
                     /locus_tag="SCK20_000499"
     CDS             complement(17833..18417)
                     /locus_tag="SCK20_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1144592.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(18748..18981)
                     /locus_tag="SCK20_000500"
     CDS             complement(18748..18981)
                     /locus_tag="SCK20_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG1144593.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(18996..19946)
                     /locus_tag="SCK20_000501"
     CDS             complement(18996..19946)
                     /locus_tag="SCK20_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1144594.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(19943..20785)
                     /locus_tag="SCK20_000502"
     CDS             complement(19943..20785)
                     /locus_tag="SCK20_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1144595.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(20787..21506)
                     /locus_tag="SCK20_000503"
     CDS             complement(20787..21506)
                     /locus_tag="SCK20_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1144596.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22198..22356
                     /locus_tag="SCK20_000504"
     CDS             22198..22356
                     /locus_tag="SCK20_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144597.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            22428..23654
                     /locus_tag="SCK20_000505"
     CDS             22428..23654
                     /locus_tag="SCK20_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1144598.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(23742..24134)
                     /gene="rpsI"
                     /locus_tag="SCK20_000506"
     CDS             complement(23742..24134)
                     /gene="rpsI"
                     /locus_tag="SCK20_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1144599.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24150..24593)
                     /gene="rplM"
                     /locus_tag="SCK20_000507"
     CDS             complement(24150..24593)
                     /gene="rplM"
                     /locus_tag="SCK20_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG1144600.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25112..25579)
                     /locus_tag="SCK20_000508"
     CDS             complement(25112..25579)
                     /locus_tag="SCK20_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG1144601.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(25699..27237)
                     /locus_tag="SCK20_000509"
     CDS             complement(25699..27237)
                     /locus_tag="SCK20_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG1144602.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27317..28003
                     /locus_tag="SCK20_000510"
     CDS             27317..28003
                     /locus_tag="SCK20_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG1144603.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28204..28731)
                     /gene="msrA"
                     /locus_tag="SCK20_000511"
     CDS             complement(28204..28731)
                     /gene="msrA"
                     /locus_tag="SCK20_000511"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1144604.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(28815..29492)
                     /gene="pgmB"
                     /locus_tag="SCK20_000512"
     CDS             complement(28815..29492)
                     /gene="pgmB"
                     /locus_tag="SCK20_000512"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1144605.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(29489..30619)
                     /locus_tag="SCK20_000513"
     CDS             complement(29489..30619)
                     /locus_tag="SCK20_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1144606.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(30619..32802)
                     /locus_tag="SCK20_000514"
     CDS             complement(30619..32802)
                     /locus_tag="SCK20_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1144607.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(32799..33824)
                     /locus_tag="SCK20_000515"
     CDS             complement(32799..33824)
                     /locus_tag="SCK20_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1144608.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(33869..34696)
                     /locus_tag="SCK20_000516"
     CDS             complement(33869..34696)
                     /locus_tag="SCK20_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1144609.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(34693..35760)
                     /locus_tag="SCK20_000517"
     CDS             complement(34693..35760)
                     /locus_tag="SCK20_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG1144610.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(35786..36622)
                     /locus_tag="SCK20_000518"
     CDS             complement(35786..36622)
                     /locus_tag="SCK20_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1144611.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(36619..37488)
                     /locus_tag="SCK20_000519"
     CDS             complement(36619..37488)
                     /locus_tag="SCK20_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1144612.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(37558..38841)
                     /locus_tag="SCK20_000520"
     CDS             complement(37558..38841)
                     /locus_tag="SCK20_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1144613.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(38855..40540)
                     /locus_tag="SCK20_000521"
     CDS             complement(38855..40540)
                     /locus_tag="SCK20_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG1144614.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            40815..41840
                     /locus_tag="SCK20_000522"
     CDS             40815..41840
                     /locus_tag="SCK20_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1144615.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(42435..46109)
                     /gene="rpoC"
                     /locus_tag="SCK20_000523"
     CDS             complement(42435..46109)
                     /gene="rpoC"
                     /locus_tag="SCK20_000523"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG1144616.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46167..49784)
                     /gene="rpoB"
                     /locus_tag="SCK20_000524"
     CDS             complement(46167..49784)
                     /gene="rpoB"
                     /locus_tag="SCK20_000524"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1144617.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            50512..51480
                     /locus_tag="SCK20_000525"
     CDS             50512..51480
                     /locus_tag="SCK20_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1144618.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            51498..52403
                     /locus_tag="SCK20_000526"
     CDS             51498..52403
                     /locus_tag="SCK20_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144619.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52400..53206
                     /locus_tag="SCK20_000527"
     CDS             52400..53206
                     /locus_tag="SCK20_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1144620.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53342..54331
                     /locus_tag="SCK20_000528"
     CDS             53342..54331
                     /locus_tag="SCK20_000528"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG1144621.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(54648..55172)
                     /locus_tag="SCK20_000529"
     CDS             complement(54648..55172)
                     /locus_tag="SCK20_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG1144622.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            55459..>56576
                     /gene="scm"
                     /locus_tag="SCK20_000530"
     CDS             55459..>56576
                     /gene="scm"
                     /locus_tag="SCK20_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1144623.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDT"
BASE COUNT        17101 a        11447 c         9642 g        18386 t
ORIGIN      
        1 ggttttagaa aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac
       61 atgtttgata tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca
      121 ttatcaacta aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag
      181 cttatagaag ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc
      241 tcttttccct ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg
      301 taaatgccca gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt
      361 ataaaaaaca agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt
      421 ttactaaaga tactataaaa tttaaacaac cgattaatgt tttaaatgtg actctccttg
      481 ttgaagatat aaaattagca aaaatccttc ctttgttgaa agaacctgaa caaatttttc
      541 ttatcgaaaa atttatcttt gataaaactg ataaagaaat tggaaaggtt ctggggatta
      601 caagacaggg tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg
      661 tttaatatct gcataataac taattttgta tgcttctttg ctcgttctcc tccttcccaa
      721 agaaatagat tataagtcca tgggaagaag gtagatttat gaattcttta aaagaagatt
      781 tcattttagc aaaggctgga aatgaagaag ctgttgaggc aattctcaaa agatttagct
      841 ctttgattca taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat
      901 gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag
      961 ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaaactat
     1021 cttctttata attataaagt atcgatttta cagcgttcta taatgattca tagaatctaa
     1081 aacgaatgat tttaattatc aacgtttttt aattcgtttt tagcttaaaa ataacttttg
     1141 tcacagtctc actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc
     1201 agaactgggc tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
     1261 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
     1321 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
     1381 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
     1441 gttaccggaa ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga
     1501 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
     1561 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
     1621 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
     1681 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
     1741 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
     1801 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
     1861 tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac
     1921 caaattgatg ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc
     1981 tatttgtcca cctatattga cttagcaact cgtattctac gttactatat ggtagattca
     2041 cacatgaaaa aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg
     2101 gtaattcatt ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac
     2161 gaccaccaca tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt
     2221 gaagcgtttc atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa
     2281 atagagatga aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc
     2341 catacaaaat ttatgatgac tccttgacaa tttgaagcga agaaaataag ggatctataa
     2401 tatctagtgt tggtgacgcc ttctgttgcc aatacttttt cttttttcta ataaacgcaa
     2461 acagacacct taaaatcctt tagaataaag tcgacgaaaa ccattcaaag gagtgtttca
     2521 agatgtccta ttcagagttt accaaagagt tactagatat tctagaccta aatcttatct
     2581 ttcatgaaga tgcctttcgc aaagagcgaa tcaatgacga aacatgtttt gtttttgatg
     2641 gcacattgac ctatcaaccg gaggaatgct ttcatcgcca ctatcaaaat gaacaaacga
     2701 tcattaaata gggctggaaa aaagtatcca tcttattgaa tgatgtcagt aactacaaga
     2761 ccattctgcg gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag acttttttag
     2821 cagaagattc agttagtgat cgtcgctgct ccattgctcg cagagtcaaa caagccattc
     2881 ttgagctatt aagcgaacct ctttctatgt ctttgatcgc acgtatgaag cacatttctc
     2941 cgaccactgt cattcgaata cttcgcagcc ttcggcctaa aaccatttct ttgagccagc
     3001 tgttaccaga agtggtgtgt ttcgatgaat tcaaatcagt gaaaaatgtc tcgggagcca
     3061 tgagctttgt catgatggac ggcaagaccc atcaactgat tgatattgtg gaaaatcggc
     3121 aactcaatcc tttacgcgac tatttcttgc gctaccctat aaaagtccgt gaacaagtgc
     3181 gtctagtggt ttcagatttc tatacaccct atcgcacatt ggtgaaagag tgttttccga
     3241 atgcccgaat cgtcgccgat cgctttcata ttagccaaca cattggtcgg gcttttacca
     3301 accaccgaat tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg aagcacttaa
     3361 agaaatactg gaaactactt caaaagaatg cgtgggaatt gaaaggtcag catcgctact
     3421 ggcgtccgtc atttcgagat catctgacag aagcagagat agtcgatcgg ctattatctt
     3481 atgatgattc cttaaagcga ggctacgaag tctaccagga ttttctttcc gttatcagaa
     3541 gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag ttgccagagc
     3601 actaccaacc agtgttcacg accttcaaga aataccaaac agaaatcaaa cgagcgttgc
     3661 gcgttcccta ttccaacggc cctatagaat gtttgaataa ccatatcaaa gtactaaaac
     3721 gaattgccta tggctataga aactttcaaa actaccgaga acgaatcttt ttatatcgag
     3781 gaaaatattt taagaaaact aagaatacta cccaactgac taaagccaga acaacaacac
     3841 ggctagacaa gatagctgtc tagccgtgtg agtatctcga ttattctatt gaatttcttc
     3901 accaacactc attgacgaag agccaaaata agtaatctaa ccgactgtta actaaaccat
     3961 tgcaggtccc gttaaagata cttgtgcctg ttgtcaccgc ttctttttct tgtccacaag
     4021 cgcttaacac acttaataaa gcccctgcta ttattaataa taatatcttt ttcttattaa
     4081 aaacctcctc atctatttcc agattagtaa atgtagtaat ctgtgctttc ttttttctca
     4141 atactaataa ctaacttatg gggtaaacaa atactcgttt gaccgggttt tgagatccat
     4201 ccagttctaa ccgcgatttg atcaggacta ttatcttctt taactcgaat tcttccatct
     4261 ttaatctcaa caatattata ttgtcccttc gcaggatgat aattcacgag tttatgattg
     4321 atcttatcca aatcgaagcg atccatttcc ttgccatcaa ttcgaacgat ggcatatttg
     4381 atagcatctt cagttgtttc attctccttt tctatagaga atacaaataa gggaataaaa
     4441 gaggtaataa tcaaaatgcc aataattatg acatcgccta tcttgacacc agcttttctt
     4501 aaaaatttca ttaacgatat ctggctaaac atattagact ttttccatgg aaatggtgaa
     4561 atttttcgga aattatctgc attaagagaa tcagttaaac aaccctcaag ttgttcctta
     4621 atacattgtt gatccatctt cataccaata aaaactagtt tattaatgcg ttcaccaaac
     4681 aacggatgcc aggcttcctt caattcggga tttttttgta taatcatttg acactcaatt
     4741 ttaggtaaac tagctaccca ataatttaat gattcaatac ttttatagct acccgatgtt
     4801 tcaaagatcg tcgaaaaatc actatgttgg gcaaaccaaa gaaacccttt tgctcttgta
     4861 atattgccag gaaaattttt atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac
     4921 cgatttcgat agataaaact tgagatacca tattcttctg tttcaggaat atgattttga
     4981 tagccaagct ctaactcttt gaaccagccc actgaatagg aagtctcatc tatattaaaa
     5041 agatttgtat ttagaatttt cgataagtct acttcacctt taattgtcct aattattttg
     5101 gcattggact gtaacttagc aattagaccg ttaattttgt aaatcgtctt ggttgacact
     5161 aagtcactat tatttaaaag taatatgtta caacactcga attgatcaat taacaagcca
     5221 cgaatatcct gttcttcttc aactacatta ccttcagctt ttctttcctg aatcttttca
     5281 cccgtttcga aattatccca aaaacgtttt acatccacta ctataataat ggcaccaatc
     5341 tctaatcgtt gggtcaaatc aatgtctaaa gggtttttag ctaatataat tgcttgaata
     5401 attggaattg gtttgctgat tcctgaagct tcaataacaa tttgattcaa gtcagaaaat
     5461 tgcactaatt gatctagttc aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca
     5521 ttcggcatcg aaacaagttt ttctccagtt cttttaaaac ctttcttgtc gactaatacc
     5581 ccatcaacat ttacttcccc tatgtcattt acaatgacag caatttcctt tccgtgatca
     5641 ccgtacagaa tatgatttag aagtatcgtt tttcctgaac caagatagcc agttaagatt
     5701 gtaataggta tttttttcat agggaacctc tttctatacc ttgaatttac actttaagtg
     5761 caacacataa aaatgagtca taatcgatat ccaactagta taagtttgtc gagaacactc
     5821 ctagaaggaa aataattatg agccagtatc gtcatcttac tatactcgaa cgagaaagaa
     5881 tctttctgct tcacgaagaa ggattttcta ttagtataat tggcaacgac attcagcata
     5941 gcccgtttac tattttacat gaactgagaa gaaacaagaa aaaaactatt caccttccaa
     6001 agcggaaaaa aattatgctt atcgaagaaa aaaatgcgga cgttatcaca ttctaaatga
     6061 tccgtgagtt tggtcaattg ttcgtcattt atttgtcgat tttcagtggt cgccagagga
     6121 aatctcgaat cgtttgaagc atgaacatat ggacttttca attagtttta tcacgatcta
     6181 acgtgcaatt tgcaatgatc ttttcgatac agaacccctc tcacacggca ataaaggcct
     6241 gatccgttca ttgcaccatc gaggaaaaac aagacataca aaaaatcatg tcgagtctcg
     6301 tggaaagatt tgccttactc attcgatcca tggccgtcca aaatccgcag atgatagaag
     6361 tgattttggt cattggaaag ccgatattgt agctggcaaa actggtggta cttgtttagt
     6421 gactttgaca gacagaaaat catgcttcct tttagcagga aagaaggtcc ccagaaagaa
     6481 agccgcttat gtgagcgaga aaatgattga acttttaagc agtattccaa atgaggcggt
     6541 caaaacgatc actccagata gaggaaaaga attctccaat catttcgcag ttacagaagc
     6601 attgaataat atgccctttt attttccaga tcctcatgcc cttggcaaca atgaacaaat
     6661 taaaatacga atagactcat tcgagaatac ttgccaaaat cgaaagagat agctacggta
     6721 gataactcaa cgataaatca gatgattgaa aaactgaata acagacctag aaagtgttta
     6781 gattggagaa caccttatga agtcttcttt gataaagtgt tgcgcttgat ttgacaattc
     6841 aagacataat aaaaagcaga tcaaatccaa ataagggaaa aatgatctac tctggtatta
     6901 tcaattaatt ggccaagcta ttatgccgtt ttgctaaacg tctacttctt ccagtattga
     6961 ccttgataaa acaacaatca ggatcccgtt ttaatactct atgaactgtg tcaatactaa
     7021 catgtaaagt tgcagcaata tcagctaatg tactcccatt taatctcatt tgctttgcaa
     7081 atcgataacg ccgataagca tcaatattct tggaatccat agacaataat acttttctta
     7141 ccatgtattt cctccaaaaa ttgaatttta atgagccatc atttcttgag caatttcatg
     7201 tttagacaat gaagctggat agaaagtagg ccaattatgc aattcattat agagtttttc
     7261 ttgactttct ccaccaaaga aaatatggaa atgctctgcc ttacttggtg caattccatg
     7321 atcactaaat tgaatatatt taaaacgacc agcacctgca tcatcagttt caaaattaaa
     7381 acgaacacca cggtttcctt tttcataatt caaaattttg tatccaacat atttgtatgt
     7441 gtattgatga tgttcaccgt tgactacaaa atcaatggtg ttgtctttaa tgttgatagt
     7501 atcaatatct gtcttatagc cagtagtata ataatctttg tactcggcag ctgtcatgtc
     7561 tttattgatt tttgctttgt aatcgaaaac ttcatccaaa attccatctt ttaacaacgg
     7621 ataaacagat tgccattcgc ctgtataatc actcaattcg cgatccttca catcggcatc
     7681 tttaaagtac ccattagcaa ctgtcttttc atccttcgct tctttttctg gaactacctc
     7741 ttcaccagca accatagttg ttttttctaa cgcttttaga ttgtcttcca ttactgaaac
     7801 ataatcttca ccattatcca tttgttcttt tgtaagactt tcaagaggat tcagtacttc
     7861 caattttatc ccagcttcgt tggccaaagt ttttgcaatt ttatcattag cattcttttc
     7921 aaagtaaata taattgatct tatttttttt aacatactct tttaattcag ctaatcgacc
     7981 agaagatggc tcttcttcag gacttagtcc agcaatagga acttgtttca atccataatc
     8041 caaagctaaa tatccaaagg ctgcgtgttg cgtcacaaaa cttttttgtt tcgcttcttt
     8101 aagactagtg gtatagtcag catccaaccg tttcagttta gtcaaatact tttcagcatt
     8161 tgtctcaaat acttttgctt ttttcggata caatttaact aactgatcct tgatatttga
     8221 aacttcctta attgccattt tgggtgaaac ccacgtatga ggatctagtt catgatggtg
     8281 atcttcttct ccgtgatcat gatcttcttc ttcactacct ggcaataaaa tcatgtcttt
     8341 tgttccttct actacattag gcccagcttt cttccaactt tctttagctt ttggtaccca
     8401 tgattccatg ttttcgttat gatatacaaa aacatcagca tcatgagctt ttgccatatc
     8461 cttagctgag ggctcatagt catgaggttc tgatcccgct ggaaccatta aatccacgtt
     8521 cgcttcatcg ccgacgatgt ttttggtgaa atcatacatt ggatagaatg ttgttacaat
     8581 ttctaatttt ccatctttat cattcgatac ttttgcatca ttagtgctgg agcaagctcc
     8641 caataatcct agagctgcta tcccaataag tcctacactg attttcttca ttctttttcc
     8701 ttccttaatt atgctaattt cttcatttta tttcgtttat gttctttgat aatttttaat
     8761 gccctttttc tagttttaat attcgggctt ttaagtcgtc gatatgcact agatatatcc
     8821 gttttctcca ttagaaatta cccccaatca ctacttatca ttatttgctt tccgccagta
     8881 atttatcaaa actctgtttg atcctttctt catccaacat ttttccgatt agaataatag
     8941 tggtttggtc tttttgtcga gtggtgtatt gaaaattaac ctgttcgttt acctcttgca
     9001 tggcaactat aaaatcctgc tcttgtaaag caatcaatcc tttaaaacga tacaggctcc
     9061 cttggttggt taaaatcagc cagtcaatcc attggagtaa aaaatctttt tttatgtctt
     9121 gatctgaagt taagagaatt gatttaaact catgtacttg atggtgttga tgatagtatt
     9181 tgccattata gtgattttct gatgaatcac tttcaaaaac taagggatga ttaaattttt
     9241 tcaaattata aaaatccttt ttttgaatcg tttcactatg tgtgaacatt cgaatatcgg
     9301 ataaagggtt aatacttttg atttctctct gtaatttttt tagtgcagat aaattcaagt
     9361 tttgttttct agacacaaaa atgcgatctg ccatgacaag ttgcttaagg gcttgtggtt
     9421 ctctttcaat aagttcaaaa ctgtcactat caataacagt caataaacta tcgatatata
     9481 ctcttcctct tagttgaggc gctgttaaaa gtgtttgaat gattggttgg ggatctgcta
     9541 tacctgtagt ttcaatgatg atttgtgaaa ttggttgttg atgttcttct attgtttcca
     9601 gtaaagaaat caatattgct aataaatcac tgcgaagact gcaacacata catccattat
     9661 ttagttgtaa cacccgttcc tcgctatgaa ctaataattt gtgatcaatc ccaacttcac
     9721 caaactcatt ttcaattata ataatttctt ccctggggta gggtgagact tgcaaggctt
     9781 gattgattag cgttgttttt ccagccccta aaaacccaga aacgattgta actggtatgc
     9841 ccattttgtc acctttttac cacttagcta tttcacttct ttaaaaattg cccgtctgcg
     9901 caattttggc gaatactttt ttaattctaa ccgttcaggg ttattccgtt tatttttgct
     9961 ggtgagatac agacgttctc cagtttcgac acattccaaa ataatatttt gacgcataaa
    10021 tcttcctcct tcaatactta tcgatcttca agatatgctt gaatttgttg tgcatcttca
    10081 aaagttaact cgccagattc caattgtgaa actaaatttt ctttcatatc aacaggtatc
    10141 tcccaataac catcgataca ttgaaataac tcctcgtcat actcttcaaa aattggatag
    10201 attcttaaaa aatcatcaaa tgataagcct tggtcctcat agtaatcttt gactaatcca
    10261 ttttcaattg cctgttgggt cggtaaaccc gtttcagtaa taattcctct ctctaccatc
    10321 gaacgataaa aagtgcattt atctagcgat tgctgggtcg tttcaagata atactttaat
    10381 ccttcttcaa ctaaaggtgg gtaaataatt tccattttga ttccctccta aaagttatat
    10441 attaaactat tttgaagctc ctttaattac tttctttcca ttatagtacc cttttaaaga
    10501 tacatgatgg ctacgtctat aatcaccatt cgcttcgtca aatgaaattt ctggttttac
    10561 tacagtttga tgagttcttc ttaaccgttt cttcgcctta gatgtttttc ttgctggtac
    10621 tgccatgata tttcctcctt ttaccaactt gcttttttta ctccaggaat caacccttga
    10681 tgggcaagtt ctctaaactt aatacgggac ataccgaact ttcgcatata ccctcttgga
    10741 cgaccatccg tctgatcacg aagcctcaga cgattcggat ttgaatctct tggcaattta
    10801 gcgagagcgg tgcgatctcc tgcggctttt aattcctgac gtttatctgc atacttttca
    10861 atcatcgcca tttgtttctt tgctttagca attttcgact tttttgccat atacctttat
    10921 actcctttta ttttgattta gacacctcaa atcgtaatcg ttacgttttg acactgatac
    10981 tttaccaaca agagttgctc ttgtcaacaa taaataacat gaaataaaac tcatatgaat
    11041 agataactat ttgaaaacga atttatcggt acatagagat aatcaaatca tttaaaagaa
    11101 tatgcagttt aggctctgaa aaataagaat tcacttttat tagtccaatc aaacacaaat
    11161 accgttttaa gtagcttttc ctctacttaa aacggcaata tgaaagaaac tataactttt
    11221 tattcaatct tttatgttta actggtttat cgccaatttt ttaaaacttt gattattcct
    11281 tatattataa gaatagacat taagtgaact aattcaatca tactgattct ttcgtgtatt
    11341 caaaattaga tgctgtttaa taaaattatt ctttttgaaa aagaattata actatatccc
    11401 aaaaattgta ggttaattta aagcctattt catcatcgtg attgagcttc tttttcatag
    11461 acgaatttct cagatagttt agttttcttt atgagatttt tgtatatttc tgaccttcga
    11521 tacaactcat tatgagtacc ttgatcaact agttttcctt gatccatcac tacaatttta
    11581 tttacctttt caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt
    11641 aaacgattta aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt
    11701 tcatctaaaa ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt
    11761 cgctctcctc cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt
    11821 agtttttcaa taaagtcttc gcagttagct aaacgagctg cttttttcac ttcctcatct
    11881 gacgcttgac tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc
    11941 tcttgaaaaa caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca
    12001 tacccatcaa ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag
    12061 atactagttt tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc
    12121 gtaaaagaga tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc
    12181 aactcaatgt caaaagattt cagaggacta tctgatccct cttggatact ggtttccttc
    12241 atagctctaa ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc
    12301 atagaatcga aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt
    12361 atagtcactt cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta
    12421 acatatgaga aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa
    12481 tgaatccttt cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga
    12541 ttaaagctat taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttttct
    12601 ctcaaagtat cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt
    12661 aagacaaaac taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc
    12721 aaaagcatca ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca
    12781 tgactcatcg catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat
    12841 aaattatgtt ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact
    12901 cttaaatgcg cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac
    12961 aaccaataca tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt
    13021 gtattttcta aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta
    13081 atgcagtata caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca
    13141 cttaaagcaa atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt
    13201 ctcataatta gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt
    13261 tccacgttct actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct
    13321 ggataatcga tgagctatca taataactgt tttttctttc atcagatgtt gaaaagcctt
    13381 ttgtagttca tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat
    13441 ctttgcatct tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag
    13501 ataaactcct ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga
    13561 ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc
    13621 tatagaaacg ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc
    13681 aattgtgtta ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat
    13741 ctgtccctta tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct
    13801 tcctgatttt cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc
    13861 taaaacacac tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt
    13921 aatctgtgtc tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc
    13981 tagtgtttcg actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat
    14041 gaaagaaaca aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat
    14101 cttgggagtg gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat
    14161 gccaaaaaat agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata
    14221 tgcaaactta gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc
    14281 accaaatatc tttataactt gaattccgcg gatatattct accgtttcag agcttaacac
    14341 atctaacgat tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa
    14401 gaatacacca attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa
    14461 gcctaaggca actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg
    14521 agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga
    14581 agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct
    14641 caaattagtc tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa
    14701 taaagctcta aagttaacca agcaacagtt tgaaccgcat aactcttagc taaatattca
    14761 tctcctacaa taattagaga ctgaagaaat ttgtaaatac tgtaatatcc atagactgtt
    14821 aatacgacag aaaaatcaga aaataaagtg gctaaccatc cgtagtattt cattttaggg
    14881 acataactaa ataatttttt gtatactttc attttttcct cccccgcatt caatttcttc
    14941 tcttattcgt ttacaactgt taatgctttc cgtaatagcg taaattagag atgtacaaac
    15001 agataaaagg taaactacgt tctatttttt gtattttttt aagacttttc ttccaatcaa
    15061 tacaccaatc atagataaac ttgctacaac taacactgaa atgattcccc atatacctac
    15121 cgcatagtgg atcattgcat ccattgttgc agcagattgt cctttatcta tccaatcttg
    15181 acgaaaacga tctgtaaaga accatatagg aatccactgt ccggcagcac tgcctaattg
    15241 cattagagcc caagacgcaa cttgaggaat tgctcgatca tatccaaata cagatagaat
    15301 aatatcagct aagatcgcca tactaatcga actgataaac caaggtaagt acgccccgcc
    15361 cattaacgta aaaaataaca ataaaacaat tgtgtaaata gtaaaaattc cttttctagg
    15421 gtagttatag ctaatataaa caaatatgat tccagataat aatccaaaaa tcccggggga
    15481 tatgctgtga ccaaacgccc caccaatact actaaccatt attgaaaaaa agtaaattac
    15541 tagggaaata actgtagcta aaccaataaa aatataatct tttgttttca tatatattct
    15601 cctcaattct atttcgttat gtcattaaaa atctgttcta tcaaagttga atcttctact
    15661 ctaagtttta tatctttttt tattccacca ttttttaaat aaataattct attgcatgtt
    15721 ttataaagaa attccggatc atgagtagct ataactactg cacccttttc ttttaagtct
    15781 ttaataaggt cagcaatcct ttccatatta atataatcca acccacttgt aggttcatca
    15841 aatagaaata aattagtttt tgacaatgaa gctatagaaa ccaataatct ttgtttttct
    15901 ccaccggata aatcaaacgg taattgatct ttcattttat aaagttccat tttttttaaa
    15961 attttttctt gctgatcttt actaactatt tcattcccca gggctaactc gctcctcact
    16021 gattctgtga aaaattggta atccatttct tgcattacta aaaacggcga tgacaaattt
    16081 tttttatttt cgattatctt tcctttggta gggcgaataa tcttcatgag tattcttagt
    16141 aaggttgttt tccccacacc gtttttccct accaaaccaa taacatctcc aactttgaca
    16201 tcaaaattta tgtcttttag aatattttca taagtaattt cttcaacttt taatacatct
    16261 gcacttacct tttcgatata gggaacttca atcgaactat taaaaatgtc aaatttcctt
    16321 aatgcgttat tgttttcagt ttttaacaac tgaatagttg tagtttttac agttctattt
    16381 ttaacaaata gaacttgatc aattaattct ttcaaatagt aaaaacgatg ttcagctata
    16441 acaatagtaa atcctttatt ttttaaatca caaagtaagt cagcaagttt cttagtcgct
    16501 ttgaagtcca aatttgaaga cggttcatcc aatataatta atttagagtt tagcgataaa
    16561 ctacatccaa tagaaatttt ctgccgttca ccacttgata gcgaaaaaat gtttttatct
    16621 aatatatttg aaagatctaa taattcagct aattcattga ttcttttatc catatgctct
    16681 tttgtaaatc caaagttttc catcgaaaat actaactcac tcgttgtatt tgttgtaaag
    16741 aattgtcctc taggatcttg acttacgtag ccaatatttt ttgatatact ttcttgtgtt
    16801 aaggattgta gcgagttatt ttcaaataaa atttccccaa ccaattttcc gtcataaagt
    16861 cttggaataa gtccattcat taactttaat agtgtggatt tcccactccc agaagaacca
    16921 gtaatcaatg taagactgcc tttttttatt gaaaaattaa ggtcaacaag cgcttgttcc
    16981 ttaacatcct tatatgaaaa agaaacattg ttaaattcaa acaattcttt cacctcaaat
    17041 caaaatactt aagaaaaaaa tagtaacgga aaacaggatg atatagtcta ataagctcat
    17101 gtttgtttta tagtaagaac ttcttttcac aggagattct atcccctttg ttattgcatt
    17161 agcggtcatt tcttcagcaa tcatagttgc acgtataaga acgggaacta acagaaattc
    17221 caaatattgc agaggctgaa aaaaagacaa gcgaatgttt ctcattttta aagattcttt
    17281 aatcattctt atttcagaac gataagtcgg aaagaatctg atcactactg taatacttaa
    17341 cattattttt ttcggtaaat gtattttttc taaagaggat aataattcat tgctagtata
    17401 gacactaacc aacaaccatg agatcattac gaccggcaac attcgaaata caatatataa
    17461 ggatgtcgca aaaaatatag taaaaaactc tatcttgata gcatcaataa ttttcattac
    17521 taaaaacaga actgaataaa tcaagatgaa ataaatacat gtctttattt tacgaaacat
    17581 caatactaaa actgtagcaa taatgaaaca ccccacttga aatatatttc cctttcctaa
    17641 aaaaataatg accggaaaaa aaatatttaa agtaatcaag gttaacggat tgatttttga
    17701 cactctttca cctccaaaaa ataagatcgc actgttatct taagctgata aaaatgcacc
    17761 gttattcaga taacagtgcg atcttatcaa aaagcataac tgagaacaat tctcaatgca
    17821 agcttttttt aattaactca gtaattcaat ccacccagga acagaaaatt gatacaatct
    17881 tctggttaat agcatagtct tttcttttga atagctatgc ttcaatatat catagaatgt
    17941 agcgtagaat gagtaaatgt agaagtggat ttcttcttca ttaaagacta aattttgatt
    18001 gatcattccg atagtcttta acttatctag acaacagact gtctttttcg tcacaaatga
    18061 tgtaattttt tcaacaatat cttgatattt ggttccagct gattggaata aaatcaaatc
    18121 gaatatacgt ttattctcaa aaatataatt aataaatgga tctaattcat tgaagttctc
    18181 tctccaactt ttctctatat ctatattttc taagtttaag aggtatttgg taaactcttc
    18241 attatacata ttaataagcg cattaatatg cggctgaata atttcttcaa ataacgcttc
    18301 cttagaagta aaatgtttat aaaaagcacc cgtagttacg cctgcggtca ggcacagttg
    18361 ccttatctta gtttgctgaa atccttcttt ttcaaaatgg cttagtccac tttctattaa
    18421 cttttggtga gttataatat aatctctggt ttctatgggt acttcctcct aataagatga
    18481 attaacttta ttatcttaca caggacttga agtttcaatc gttaacgttt attactgaac
    18541 tcattcctta gagctaaact aatgagaaag agcagaataa ctgatacaaa aagcaatcct
    18601 acaagaatta atagactcat ctaaatcatt caccttttct aaaataatcc gagaaagatt
    18661 taaaaaaatt atatccagct attaggataa gtcctatcat aatcactcca atagccattt
    18721 tcttatcctc cctaaaaata aagtctttta cttaaaacct tttttgtaat cgtcaataaa
    18781 ttttttccct ttatcagtta attgaaatcc tatatctaaa tagtcctcat atctaagttt
    18841 agccagcatc tcacttacag atgctggact aacacttaga acttttgcta aatcattatt
    18901 ttttaatttc ttttttcttt ttgataattc ctgaattgct agtaaataaa ttagtatagt
    18961 agtcgtttta atttgtacca tacctaccct ccaaattatt tcgaaaggcc ttcagcaatt
    19021 ttatccaggt tccatttcat catcgcatag taactatcac catcttgtcc tttttctgca
    19081 attgaatcag taaaaatcgt tgaatagata ggaatattgg tatcttttga tactgttttc
    19141 attggtctat catccacact gctttctacg aataaggagg gaacttttgt tgtgcgtaat
    19201 ttttcaacta agtgttttat ttgatctggt gttccttctt cttcagtgtt gatttcccaa
    19261 atgtaagcag aaggcacatt atacgctttc gagaaatatt taaagcatcc ttcacttgta
    19321 acaatcattt ttttatcatt cggaattgaa gcaaatttag atttagcttc tttgtctaga
    19381 gaatccaatt tttcaatata cttatctaga ttttctttat agaatttttt attatcagga
    19441 tctttttccg ctaactgttt ttcaatattt ttagcgtaaa taataccgtt ttctaaattt
    19501 aaccaagcat gaggatcttc cttccctttc tcactctgac cctctaagta aataacatct
    19561 atgccatcac ttgctgcaaa atagtctttg ttttcctctt tgttcgcatt tttaactaat
    19621 tttgtaaacc aagcatttcc tccagtttcc aagttaacac cgttataaaa aatcaaatct
    19681 gcttttgaag ttttttgaac atcttcaggc aaaggttcat attcgtgggg atctttccca
    19741 ataggtacga tactgtgtaa atcaatttta tcttttgcta tattttcggt aatatctgct
    19801 aaaatcgaat tagtaactac tacttttaat ttttcgttcg aagccacttt atctttgtct
    19861 ttactattta ttgaacatgc cccgaatata aaaagagcaa tcaaaatccc cagacttccc
    19921 agtactgctt tccattttct catcataacg atctctcctt ctttcttaaa aatttttgtt
    19981 ttggtgagat tagaaaacta attacaaata agaatgccgc tgttaaaaca atacttgatc
    20041 ctgctgctat gttaaaacta taaccaataa acaatccaag caatgatgaa cttgctccga
    20101 ctgttgcaga aataaatatc atagttttca agctatttgc atataaatat gctgtagctg
    20161 ctggtgtaat taacattgca acaattaata tagttcccac actttgcata gctgttactg
    20221 ctactaaagt taatagaacc atcaatagat aatgataaaa actgacattc atccctattg
    20281 cttttgccat taatggatca aaagaagtga ttaataattc tttaaaaaat agaactacta
    20341 taaccaacac taaaaccgaa acgccaattg taatccactt atcaatatct tgtacagcta
    20401 aaatatttcc aaataaaata tgaaataaat ccgttgagct atttgcaact cctataagga
    20461 taactcctag tgctaaaaaa gaactgaaag taataccaat tgctgtatct ccttttatta
    20521 cactattgct ttttataaaa gtaataatga tagaagctaa tagaccaaat acaatcgcac
    20581 caataaaaaa attaatacct aaaatataag aaattgctac tcctggcaaa acagcatgag
    20641 aaatagcatc acccataaga gacattcctc ttaaaataat aaaacaacct actgttccag
    20701 aaacaatccc tataactaca gatgtaatta gagcattctg taaaaagtga aagtcataca
    20761 gaccattaat aaaattttga agcatattat tccccctctc caataaatat agtatctcca
    20821 taggcttttt ttaaattttc ttcattaaat acagattcta cacttccgtg agcaattaga
    20881 tttcgattca aaagaataat attatcaaaa tacttcttta ccttgcttaa atcatgatgc
    20941 acaattagca ctgtttttcc ctgatgtttc aattttttta acgtatccat aattattcgt
    21001 tcactaacta agtctatccc tacaaatggt tcatctaaaa atatgaaatc cgcattttga
    21061 gcaagacatc ttgctaaaag tacacgttga aattgtcctc cagataattc tcctatttga
    21121 tgattagaat attttcccat atcgaccttt tccaaagcct ttgaaactct ttgccattct
    21181 gcatttttaa ttctttgaaa aactttcatt ccggcatatg ttcccataga tacacattct
    21241 tttactttta ttggaaaagt aaaatcaata tcagcttttt gctctacata tgcaatcttc
    21301 accagttttt tattgatatc ttcattatta atggagacct ctccctgatg agggataata
    21361 tttaacattg ctttaagcaa tgtggatttt ccagcaccat ttggaccaat aatacctgtt
    21421 aaagcacctt cttgtatttt taaagaaatc tcgttcaatg ctaaaaaatc gttataacaa
    21481 acattaagct ttttcacttc taacataact ttctccttct aaatgataat gattatcacg
    21541 atcatatggt atacaaaaat tatattttag tcaaccctaa attttatagt ttagattttt
    21601 ttctgttgct gaagattaga atgacaaaac tatttgaaac tacgcgtata gtaagtagct
    21661 ttcaaaatat ataaaaaagg agcgttctca aatgagaaca tgctcctaaa actactttat
    21721 tggcgtatgg aaaaattata cttgaagtgg atagcgggtt gaataagttg ataagcatca
    21781 aaacatttat aaaaattatt caagactatg tctgttccat atacaaatat gaaaatcagt
    21841 aaactccatt taactttttt cgtagtgtgt ctttttatac atgtttatcg ttattaatag
    21901 tgaataggaa aaaaactatt tttgatcatt gacaattaaa tacaattttt gaataacgat
    21961 ccctgctatt cgagctagaa actcaagtac gccatgacct gattagcgac aaatacttag
    22021 agatttcttt ttgttctggg aaaacaaaat gccatgacaa tagcagcaaa taatgatact
    22081 tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta cccaaagcaa
    22141 ttcaaaaatt tcataactac tattttagga gatgatttta tagaatccag agtctacctg
    22201 aataaaaaac aaacctgtgc atttctagga atcgataata atactctaga taagtggatt
    22261 cgtatatata atttcccagt aataagagtt ggtggtgtag ttcgctttga cgtagaagat
    22321 attaaagact ttatgaatac atttaaaata acttgaaatt ttcttctcgc cgcatatact
    22381 aaaattagtg aaatatactg ccttactttt tttaaggagt gaggattttg cctacaaagc
    22441 tttcaaatgg gaaatataaa actaatctga gatatccaaa aaggtttaaa gaaataacag
    22501 gaattgcatc agaaaagtat caaaaaacat ttccaaatag gcaattggcg attaaagccg
    22561 aaaatgatat gaagaagaaa atagaaaaag ttttgagaga ggagaatgca aactcattag
    22621 aattaaaagg aaaaataacg tttaaaaaat tttacgaatc taaatggctt cctagatatg
    22681 aattaggtca aacaattaga tcaaacaggc ctcctagtga tataaccatt agtaatacaa
    22741 aagatatatt tcggttgcat attttaccta tgtttggtga atacgcaatg aattatctaa
    22801 atatcaatac tgagataata tcagatgaat taaccaaaaa atcgaaagaa tatgcaaata
    22861 taaaaatcat aaaaggatat gttcgttcca tgttcgatat tgcagaaata ctaaattata
    22921 tcgaatttaa tcgaactact aaaattattc aaagtatcac tgcacctaaa aaaaatgctt
    22981 tagaagaaaa aagaatacaa gaaggtaagc aagcactatc atcaaaagaa cttactaatt
    23041 ggatagaagc agtaaatgat gattttaata atcatttact tacttttcat gattacacac
    23101 tttttatgat taccctgtat cttggtgata gaaaaagtga gacttacgct ttgcaatgga
    23161 aatatattga ttttgaaaaa caaacggtcc gtcttaaaca tactttagat aaatatcaaa
    23221 gaaaaaagtt tacaaaaggt cgaaaagata ccgtcattca agtaccagaa gttgtaatga
    23281 cattgctaag tgaatggaaa tcggtacaag ctgatcaatt gttgaaatta aaaattaagc
    23341 aaacgttaga tcagtatctc tttacttata ctaaaccatc tggagaagta aattgtcctg
    23401 ttcatgccga ttacctaaac tatcgaatca attcaattaa aagaaggcat cctgacttag
    23461 ttcatttatc tccacacaaa ctccgtcata cttatgcaac tatagctaga caaggtggag
    23521 cagatatgaa tcaaatttca aatgctttaa cacactcaga tatttctaca acaaagatat
    23581 atgtgaacac accagatata gttgataagg ctgtttttga agcttttcag agaggattga
    23641 ataagtgtga ttgatatgtg ttcttaaatg tgttcttaaa aacaaaagaa gcactttcca
    23701 aataattgaa aagtgccaga aacgttgata tataaaggat attaacgttt tgagaattgt
    23761 gaagctttac gtgctttctt aagacctggt tttttacgtt cgaccatacg tgcgtcacga
    23821 gtaagtaagc cagcacgttt aagagctaaa cggaagtcag gatcaacttc tagcaatgca
    23881 cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc gtttacgtta
    23941 acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt gattacttca
    24001 cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt aattttgcca
    24061 gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt gccgatatat
    24121 tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc taaaacttct
    24181 ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt tgtaaattgt
    24241 ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat caaacgacga
    24301 gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg gtagttgctg
    24361 tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt gtctgcattg
    24421 atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt atttttacca
    24481 cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt tgcgtctact
    24541 acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg cacgttattt
    24601 tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc tctttgtggg
    24661 gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg tttttctgct
    24721 tgattgaaaa atttatatga aactttactt cttttctttc ttttatataa aagggtcgtt
    24781 tcctgtcaaa atgaaaaaca aaaagaggct gtgacaaaag tcttgtcaca accccttatt
    24841 ttgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccataaaaca
    24901 taagaagctg ttttttaaaa ttgtttcttg tgactcggag ctgaacggca ggcttacaac
    24961 ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa acggcaaaat
    25021 atgaggaact attttgacgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
    25081 cctctttttc ggtcaagttc aacaacagtt tttacgcgtc gatctctggc gctttgccca
    25141 aatgagcttg gatcatccag atacgttttt ctgtggctgt tttgaattcg ataaagatat
    25201 cttgtgttgg atcgtcgcct tcttctccag aaacttcgat tcctttttgg taaagatctg
    25261 ctagataacg atagcctgca acaagtgttt ccaaacgttc tggaatagta cgatcccagc
    25321 ggccaatttc atcagggatt tttgtgttat ctgcaaattc acgcaatgta gaataaggtg
    25381 aaccgtctaa tgtgatcaag cgttcggata cttcatctaa ctgagcgttc aaatcatcca
    25441 tgaactcatc catcattggg tgcaatgtaa ggaaacctgg tcctctcatg taccaatgtg
    25501 tttggtgaac aacaacagac atttggctta gatctgcaac taattgattt aatacttctt
    25561 tggtttgttg gaatttcatc tttcttgcct cctaacattt tcttacactt aaaataatag
    25621 tagactttca atgaaaagta caacaaaatc atttacctga gaacgatttt cagttcgctt
    25681 ttttatccgt aaagccaatc atttttcaga atcagctaat tcttctcgca gttgagcaaa
    25741 ttgctgataa gtctgtgcta aatgcttttg tgtcgaaaca aataatggat agattttctg
    25801 atatttttta tgatcagttt cttgtactcc aacttgctct ttcaattcta tcaattcctg
    25861 tgcttcactg atactttgga tctttccgat gcttctccag cctaaaatca cagctcctaa
    25921 tgcggatgct tctgtgacag tagggaaaac aagcgggtaa cccagtacat cagccatcat
    25981 ctgacgaaaa atcggagatt gagcaaaccc gcctgtagca cggatctctg tgagctgatt
    26041 ttcctggcaa actcctttta aaatccgata aaggttgaga cagatccctt ctagaatcgc
    26101 cttcatcatt tctttttctg tatgttgccg ctgtaaaccg ataaaactgc caaacgcatc
    26161 tgcgtcccaa agcggtgctc gctcaccaag taaataggga tgaaaaatca gaccattggc
    26221 acccgctggc acaaaagcga tagccgaaag cagtgcatca tatggatttt ctcctttttc
    26281 tttagcagat tgaacgactt cctgtaaaaa tgtatgccct gcccaatcaa agatgccagc
    26341 accattgcta gtcgctcctc ccactaccca atgtttctga tccagaacat aacaaaacgt
    26401 ctccatttct ggatggaact gaggctgagt agtaataaaa cgaagtgctc cgctcgttcc
    26461 tacggtcaaa gcggcgatcg tatcaccaaa agcacctaac cctagattag aaagcggccc
    26521 gtctgcccct cccaaaacaa atgatatatc atttggcaaa cctagttctg ctgctgtttt
    26581 tgatggtaga cctgttactt gataggtgga aggaaagact tgcggcaatt gctctattcg
    26641 aatgttcaag tagtcaagca cttcttctgc ccattggaat tcatggatat caaaatatcc
    26701 agttcctgaa gctgttccgt agtcgctgat cagttcgcca aacaatctat aaaagatata
    26761 gtctttgata ccgacaaaat aagcagtttt tttgaataat tcaggttggc tgttttgcag
    26821 ccacttgatt ttgttcaatg gtgtcattgg atgaatcggc gtcccagtct gccgataaaa
    26881 ttctttgcca agcacggtat atttcagttt ttctgcttcg tccgctgctc ggttatctgc
    26941 ccaagtgatc attcgagata acggtgtttt ctcttcatcc aacaatatca ggccatgcat
    27001 cgcagaggag aaggagacaa attgaatgat tgtgtctgaa ggcaactgtt taattacttc
    27061 ttttataacg gtgcagacac cttgaaagat caattcctga tcttgttctg ccatcccttg
    27121 tgtttcttgg atcaaagggt agcgaaaata agcggatgct gcttcttggg cttcatctgt
    27181 gaaagcaact gctttggttt gtgtggttcc gatatctaat ccgattgaaa cgttcatttt
    27241 ttcacctcaa attttatgta tgtacaaaaa tttagtttaa gcttgcgtat cttggttttg
    27301 aaaaggggga gatggcgtgt tactttattt tatcattggt tgctgtatcg gatcattttt
    27361 atgtttaacc gcccagcgga ttccgttagg tcactctatt atttatccta gatctcattg
    27421 tgtcaaatgc tgtcgttcgc tttcttggta tgaattgatt ccgattctct cgatcatcgt
    27481 ccaacgattt cgctgccgct attgcaggtg tcggttgccc ttctactatt tgttggcaga
    27541 agccttatgt ggtggtcttt ttgcctggtt ctttactttt tcttccgcaa gaaatttttc
    27601 gacgttcata tggatgcttt cggctttgct attttcgttg atggatttat tttacttcat
    27661 ggtccattct cacacgctat tctgttcttg ggcaatttta tggatttttt ggttgcaggc
    27721 cggcgtcttc caatggcaaa gtgtgctgtt aacgtttatc gtaggcggtg tgtgccttcg
    27781 ctatggtcaa tcctatctcg gagctggcga tactctgttg atgctttctt ggagtggtgg
    27841 cttgtctcta gaagagttgc ttcagatctt gtttcttgct agtctgtttg gaatatccgt
    27901 tttttctgtt tactttatct gtcataaaaa aaagttggaa aagctgcctt tcattccttt
    27961 tttgagcagt gcgttgctga ttgtccttca tcttaggtat tgaaaaaagg ttgtgacaaa
    28021 cgacttgtca caacctctga tatatagtgt tctcttacca aatccttgtg acttggagct
    28081 aggcgacagg ttcacaacct ctccactaaa tggtgttcaa tcagctgacc ctcagtatta
    28141 gctcaaaatg ctggttttac aacctctgat atacggtgtt ccataagtag ctttttcggc
    28201 ttcttatttc ttgacgcccc aatgtcgacg aaggaaacct gcacgtccag aaccttctcg
    28261 ataaagattg taatggagct tgtttttctt gtaataatcc tgatggtaat cttctgcagg
    28321 ataaaattcg cttcttggtt cgatttttgt cacgattggc ttatcgaatc gcccgctgtc
    28381 ttgcaatgct tgttttgaac gctctgcaat atttcgttct tcttcatcat tatagtagat
    28441 cactggacga taagaatcac cgcgatcagc gaactgtcct aatgcgtcag tcggatcggt
    28501 ttgctgccaa tagatttcca ccaactgatc ataagaaata acttctggat catattcgat
    28561 ctctactgct tctgtatgcc ctgttgtgcc tgttgttact tgttcataag ttggatttgg
    28621 tacatgcccg ccagtataac cggatgtcac agaaatgatc cctggctgct cttcaaatgg
    28681 tttgaccata caccaaaagc atccgcctgc gaaaactgct ttttttgtca tataaaatcc
    28741 cccttttttc tcatcatacc ttgatttcca aaaggaggat aacgttttgc ttgtgacaga
    28801 aaaaacggaa actattatat acgtgatacg gcttctgata aatgccaaaa cgaacgtact
    28861 tgatcaaaaa cggttaaatc aggaatcaca tattcagcag aaggaagatt tatgttttca
    28921 cctattccca atacatgcat gttaccagcc aaagctgctt gacaaccagc ttctgaatct
    28981 tcaatgacca agcatgcatt tggcggtaca ttcaattgct gtgctccttt gagaaaaact
    29041 tctggatcag gttttgcttt tgatacttgc gtgccgtcaa taaggacatc aaaataactt
    29101 gtcaaattcg ttttttctaa aatgaggcgt gcatttttgc ttgctgatcc caatccgatc
    29161 ttgatgtttg tttttttcaa gtactcaaga acagctattg caccaggcaa aacggatgtt
    29221 tcatccattt tttgaatcgc ttgaagatat agattgtttt tttgctcagc aaaggctgat
    29281 ttctcttttt ctgagaattt ctgttccatt tttccaaatg acagcaatcg ctctagtgac
    29341 tcggttctgg atattccttt cagttgctcg ttttctttct cagtgaatgg aatggacagc
    29401 tggttagcta aaaccagcca cgcttcgtaa tgataacgag ctgtgtctac aagaacgcca
    29461 tctaaatcga atagtgctgc tttgattttc atcgattacc tccttgaaaa tgaacgactt
    29521 gaagtaaaga tgcgatgttt ttagctgtgc gatgaagatt atcagtggct tgctgtaaag
    29581 cttcttgtaa aggcacagct gcaggaagga tcggaaaaac ggccgaaatg ccaacatctg
    29641 ctgctttttc gcttccttta ccaacacttc cgcaaatagc tatcacagga acgccctctg
    29701 gtactcttct agcaatccca actggcgctt ttccttttag actttgtcca tctagacgtc
    29761 cttcgcctac gattacgagg tctgcttgct gtacccgttt atcgaactcc agtaaatcca
    29821 atacgtagtc gatgccagag actattttcc ctgaagcaaa tgctaacagt cctgcaccca
    29881 ttccaccacc agcactactg cccgatgcct cgattacttg aggggcaaat ctcatataaa
    29941 aatcatgcat ggcttggtca gccactgcta attcattttc tttcaagcct ttttgtggtc
    30001 cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt agtaacatca gtaatgacag
    30061 tgattttggc tttaatcagc tcaataggca cgttcgtttt atcaactgta gcaattttac
    30121 ctagattttc tcccagtgct tctacttttt gattatcttg atcgtaaaaa gcataaccta
    30181 gaccttctgc catgccgatg ccgccatcgt tggtactact tccgccaacg ccgatgatga
    30241 tgtgtcgaac accttgttgc aacaattgtc gtatcagttc tcctactcct cgcgtggata
    30301 acctaagtgg ctgttgttgg tttagtggga catctttcaa tccgacgatt tctgccattt
    30361 caaaaatcgc agtttgaccg tctgaagcgt actggatttc tttgtttttg ccaaatgcat
    30421 tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat gaaaccgttc
    30481 cttctcctcc atcgcctata ggtagcagat catattcagc ctgaacaaaa acttcttgaa
    30541 aacctgattg aatggcttgt gctgcctcta aggctgtcag actttctttg aaagaatctg
    30601 gcgcaatcag aatacgcatt attttcccct ccgttccaag atcagttctt tttccagctg
    30661 gtattggtct ccattgacaa aaataggtaa tgtttctttc gtctcttttt ttattactat
    30721 ttgttttttt gtcaaaacaa atagcaacct gtgatttttc caaataaaag gaaaagaaat
    30781 tttttcccat gctgaaggaa gtcttggaga aatagaaaga gttccattcg ttactgtaat
    30841 acctgaaaaa ccaaatataa ctgctagcca tagtgctcct aatgaagcag catgaattcc
    30901 gtcatctgat gaatgcggat ctggtcctaa atcaatcagg cacgcttctt ggaataaatg
    30961 atacgcccat tccatttctt ggatacggca agcctctatg gcgtgaatcg ctttactcaa
    31021 cgatgagtcg tggattgttt tgttttcata gtaatgcaaa ttcttagaaa cgattggttg
    31081 agaaaaatac tcaggtaata agtataacag catgacaaga tctgcttgct tgctgacttg
    31141 gagttcattt acttcgctac gggcataatc aagtaaaata gactggctgc caggacgttc
    31201 tttgtaagtg gtcaaatcaa taactggctt actgagatac gtgtcatctt gcggaatcag
    31261 ctgttctttg ttaggcactg gcaaatataa cttatctaaa aaattttgac attctttcaa
    31321 aaaaagcgga tcgccagctt gatatttaac agctaaggcc acattataat gcgccagata
    31381 gttcgtatat gcattattgt cgatatattc tgtgtattca tctggaccaa tgactctgtg
    31441 tatctctaaa tgatcatcga cttttacagc tctgctcagc caaaaagctg ctgtttcatg
    31501 aatgagcgtt tttccctttg tttctaaaaa cgattgatcc tctgttgctt ggacatactc
    31561 ttgaacggca taagcaaggt ctgcactgat atgatgttct gcggcagctg aagcaacttc
    31621 ttgtcttgtt ccggttttga tattcaatgc agcaaatttg ggtgtctctt cctgaccagt
    31681 gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga taatctcttg ctttttgccg
    31741 tgcgccttct aatcgatcat aacggtacag cagcaaattc tttgctactt caggaaactg
    31801 atataaaaag aaaggcagga taaaaatttc cgtatcccag aaaacatgcc ctttataacc
    31861 ttcgccagtt agtcccttcg cccctacact aacacttgga tcattgcggg gagtcataat
    31921 ctgtaaatgg taagcagcga agtccatcgc taactgatcg aaagaagaat cgccttggat
    31981 cgttatccga tgctgatgcc aaaaatctgt ccaataagac atcgactgct ctaaaatttc
    32041 tgtataacta acgcttttct gggcttgggc aatacttttc ttaaacgggt ctgtttgctg
    32101 aagagaggta gagagcacga tgatcttctc cagcgtaaag gacgtatttt ttctgatctt
    32161 ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg aatacacctg ctttattgaa
    32221 tttagctgca atcccaatat caattttact ttctgttgtc tggtaggatg cactgatcaa
    32281 atcgtcacca aatactcgca gttctttttc aaccaactgc tgtgtaccaa aattggtttg
    32341 ctgtccatcg atacccgttt tcacatctac ttggcagtct tcggtcaacg gcttgatgac
    32401 cactttcagg gctgcctgat ggagattatt ttgatcagca acacgacaag taatgacttc
    32461 cacttttttt ccttgagcag tttcaatgag cgcatagcgt ttaagctctc ccatttctaa
    32521 atctagtgcc cgctcgtatg caagaacttt catcgtctca agagaaaaat gttcaccgtc
    32581 gatttttatt tcccatctga tgacatctgg gagattcact aactcggctg attctgctcc
    32641 taaaggtcgg ttatatacac cagctgcaaa cattcccctt acttgattcc gatagtcttc
    32701 ctcctggctt cctctgatac caaaaaatcc atttccaatg gtcatctgac tcgcatactt
    32761 attgatatac tctaaatcaa actgtttttc ttctactttc ataaggtaat aacttctcct
    32821 gtttctgccg aacgatataa attttccacc agattctgga tgacatatcc ttgccagcca
    32881 tctgcaatca tctgagtctg gtcaccgagc gcccgattca caaaatgagc catacttagt
    32941 tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc atctacatct
    33001 tcgaatagtt cgcctggata cagattagca cctgcctgat cgccacaaaa agccacattc
    33061 agtttgtctt ctctgatatt caaagcaaac gaagtattca gttggatgat cttgccgttt
    33121 tctaattcca gtgtgccaaa aattgcatct tctactgtga atttgcttgg atcccatttc
    33181 ccgaatttcc ctgaattttt tttcgtacca attttttgga agctgtacgc ccgaacgctt
    33241 tttactttag gaaatccagt aatatacatc gctgtatcaa gcatgtgaat gccataatcg
    33301 atcaaaggcc cgcctccttg aagttctttg tcgataaacg ttccccagcc tggaacacca
    33361 gaccgacgaa gcgcattcgc ttctacgtaa taaatatccc cgagaacatc aagatgattc
    33421 ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc cataagccaa aatctgcccg
    33481 tttttatcgg ctgtctctgc catctttctt gcttgatcag ctgtcatagc tggtggtttc
    33541 tcgcacatca ctgaagcatt agcttcgagg gcagccaata catgttcgaa gtgaaagcga
    33601 ttgggtgtac agacactcac gatatcggga gaaatctcat tcagcatttg tgtaactgat
    33661 gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg gctgactgga
    33721 tcaaccacag cggccaattc taagacatct gatctttttt gataataagc agcatgtact
    33781 ttttctgcta cttgtccagc gcctatgatt gctactttct tcatttattt ctctctcctt
    33841 tgcttattta cttattgtta ttctttctct attctttctc taaatcattt aaatgacgcg
    33901 ctgtttccag acagtttttc aagtattgca atgattcctg ataaattttc acttcatttt
    33961 ctcctcgcaa acgacattca tataccatat aaccttcata ctcattttga agtagcgttt
    34021 gaaaatggcg agtaaaatca attgcgccag atcctggttg gtagcgatga ttatctgcta
    34081 aatggatatg acccacatac tctttttgcg tagtcaatgc ttttgaaata tcatcttctt
    34141 caatattcat atgataaaaa tcagcgatga tctgcacatg tttcaattga ttagagtcaa
    34201 tgtaatgacg cgcatccgct aacaaattga tcatatggtc ttggtatcgg ttcaaaggct
    34261 ccaaataaat aaccgtacct gtttcttcgg ctactttatc taaataacga agtgattcgc
    34321 tgactgcttt atagtctcct tctttgcttc ggggtgaaac catcggcggt aatcgataag
    34381 tgaacattcc ccaagctgct gggataacga ttcctttgcc tccaacctcg cttagtgcac
    34441 gcaaaatctc agcaatttga cgtacaccat taagacgccg ttcttcgata aagtcaccga
    34501 tccacccatc gtagccgtta caagctgtca ctactgggat ccctgtttct ttgattgcct
    34561 gtttgactgt gtcgatattt tctattaaca acttaccgtc gatttcgtat tcttcaaatc
    34621 cacagtcttt gataaatcta aatttttcta aaatgtctct tgggaaaaat gcttgatctt
    34681 gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg cccattttga tactttcttc
    34741 tgggtgctgg tctacataac gcatgtaagc ttctgcgctt tctgcaaatg gcacaactgg
    34801 atcaatgatt tcttcacaat ccaagtaacc gttcatcaat aattcccaac atgtgtcctc
    34861 gattcgttta cggttccatc ttgggtaatc tgggtttggt tcactagatg ctcgagcaaa
    34921 gacgattttt gcattattga aatgtgcttc gcgaccaaga ttaaacccag ctggaaaagg
    34981 tttagcaaag gcaacataag aaatgatccc gccatagccg atccctatta aagaagcttg
    35041 taatgcggca gcactgccac tcgtttcaat aactgaatct actccacgtt tatctgtcaa
    35101 ttcctttaga cgaaaaccga catctgcttt cataggatct agcgtgtaat cggcaccgtg
    35161 tttttctgct aattctctac gctgcgctat gggatcgatc ccaacgatgg tagtagcacc
    35221 tgctttttta gctaattgaa tcgcaatttg cccgatagcg cctaaaccga agattgctac
    35281 agcatcacct gctcgaacat gtgcttcccg gataccacct aaagcgaatt gagcaggatc
    35341 gtaacatact gcatttttcc attgatccaa ccgatcaagt ttacgtagtt tataattatc
    35401 aacaccgttt gtaatcagcg tttcacgaat gggtccataa gtgcagacta actctccttt
    35461 ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca tatttcctaa
    35521 ttgaaattcg ccaaatacca caccgcgttg gcttccttct tctcttggca agaataggcg
    35581 ccattcttct gaaaattctt catcgataaa tggacttaat cctctaaagt cgacaatttc
    35641 tgttcctggt tttggagtag cgaaaattac tcggatacgt acttcgttct tttcgatctc
    35701 acggtcttcg taatctttga atccggctat tcttggttct aatgcaacta attttttcat
    35761 cttttctttc ctctttcttt tctttttatc cttttacgcc gccttcgact aaatcacctt
    35821 taataaatcg ttcagataac gcatacataa tgatgactgg taaagcggtc accaatgaag
    35881 cggccatcat tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc aaaccgattg
    35941 gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc aaataaataa tcattccacg
    36001 aaaccataaa gcaataaacg aagacagaaa tgattccaga aatagccaat ggtataatga
    36061 tccgaataat gatctggatc cggctaagac catcgatcat tgctgcttct tcaatatcag
    36121 tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc agtaggaagt gtttgaacga
    36181 ccatcgtgat aatcagtgct gtattggtat catacaaacc tagaccggaa atgattttaa
    36241 ataatggaac aacgatcaaa ataccggaaa acatataaac ggtgtaaaag ctagcattga
    36301 ttttcgagcg agctttgaag cgtagttttg acaaagcgta tgccccgctg atcccaatca
    36361 ctactgcagt tacggaggct atcaaggaga tcactaaact atttttgaaa tacatgatga
    36421 atggaaaaat ctcagcattg aaaatatcct gataatgttc aaaagtccat tctttcggaa
    36481 aaatagtttg tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc atcagcatga
    36541 tcaaaaatgg aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa aataagattt
    36601 tcctgctctt tttatttttc atttcaagtc caccttcttt cgtgagagca agacgaccgc
    36661 tgcaataatg atgaacaata gaatcgatat agctgctgct tttccaagat cattgaacgc
    36721 aaaggctgtc tgatacaaat aaacgcctaa gatattgact ttattcgtca gtagatagac
    36781 atctgtaaac atatagaaca tccagataga tcgtaaaaca atcacagttg ctagtactgg
    36841 tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac cgtccatttc
    36901 agctgcttca taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg aaataaacgc
    36961 atacggatag tatctccaga tagcaaacaa aacgaccata aagaagctag caactggctg
    37021 gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta tctactaaca gatcattgac
    37081 gattccataa ctattattga acatatattt ccaagcaaaa atcaaagaaa tagatggcgt
    37141 aacataactt aataaaatca gtgaacgcac cgttttccta aatctgaact tgcggttaag
    37201 caaaatagct acggctaatc ctaaagctgt actgcctgct acaactaaga atgtgtaagc
    37261 aactgttaaa cctaaagaac gataaaattc actatcactt aaaatttcaa gataattgct
    37321 taatccgata aaaacggcgt ctgagttagg attcagcggc atatcgaaaa aactgatctg
    37381 aatattggaa agcattggaa tcagcactaa aacagctaat aaaagcatgc ttggtgctaa
    37441 aagtcccatg gctaatttta agtcagaatg tttccttgga cgattcattg aaacctcccc
    37501 ctgataatct gccaatagct atctactttt ttcgaacacc attggcagaa cttcttgtta
    37561 tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc cgacagttac
    37621 ttggttcact gcttttccga tcacacctga gcttgtgata tctcccattt tcgtgaaatt
    37681 cttttccccg actagtccaa agacttgtac ttcatcaaaa gcactagcaa tctcagatgg
    37741 cagctcgcca aaagacttga tgacttcatt ttctttgtaa ccatccagtt cgactacttg
    37801 tttattcact ggttgagcac caccaggaga catcaagacc cacttttcca tgtttttcgg
    37861 ttgagataag aaagctgtga atgcttcagc agcctctttt tgtttctcgt ctagaccaga
    37921 agaaatcgtc aatccagata ctgtgccata gacagcttga tctttttgtg ttgggatagc
    37981 aaaaccgata ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt aagttgaata
    38041 gattgccatt ggtgctgttc cattcatgaa agcatctttg atttctgtca catcattaga
    38101 gcctggcatc gtgtattggg ataagttttg ataaaacgac aatgcttctc tcatcttttc
    38161 cgtatcgatc gttacctcgc cttcatcatc caaaacattt gctccatttg acaaagcaaa
    38221 ttgagagaac gcttgctcac tcattgttcc ttcagcagta ggcatagcaa ttccatattg
    38281 tttattctct ttatctgtga aatgttgagc aatttttaaa atatcatccc aattttctgg
    38341 ttctgaaaaa ccagcttctg ataatttttc tttgtcatac caaatacctt gaacccagcc
    38401 gctaattggt gcagcaatat agctttttcc atcttctgaa cgaacaaggt ttttggctcc
    38461 gtcataataa ttatcttctc cagcttcctc gataacgttt tggactgccg tctgatcaat
    38521 cagttcatct ttatccatca ctttagcaaa atcctgactg acttccatta cttctggtaa
    38581 tttaccggaa cgagctaacg taacgatttt tgtattgaac gctgattctt ctaccggcac
    38641 ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt cattgatcac
    38701 acttaatcgt tcctgttcga cagaagagtg catgaattga atcgttgtct gttgtgattc
    38761 gttagagctt tgactttcac cacaagcagt aagaagaaat ccagtagcta gcagacaggt
    38821 agtaacaata atttttttca tttttttcca cctcttattt tattattttc agccacatat
    38881 attcatacgg ctggaatatc aactttcgtt cttgactgca atggataatt tgtccagaaa
    38941 acaggtctag gtaatctcct tttggtaaaa gataacctgc tgattgattg gaaaaattat
    39001 ggataactaa gactttctct tccttcgctt cgcgaataaa actgataaaa gaatctgctg
    39061 agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt cgaatagata
    39121 gcatctgggt caattcacca tatactgctg ttcttaatgg atctgtctgc aactctttct
    39181 caatatctgc taacaaaaat ttttgccgat tgatttttcg attctctcct gttttttcta
    39241 cgcctttgta atcatttcga cttcccagca cactttgaat ataaattgcc ggcacgcctg
    39301 gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt gcttctccat tccgtcgtat
    39361 ctgttggaga acttaacgca tctagatacg tagcgtttat ctcatatgga ctggtcgttc
    39421 catctgggtt tttcttgtag tttactcggg caccctcttt cttcagctgt tccactaatt
    39481 caaggatcgt tttttccggc agaatacctc gtaccggatt caatccgata ccgtcatggg
    39541 aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt tttaaccatt
    39601 ctttcaactt acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt ggtagcgaaa
    39661 actgatagac catctgtgct tcattctcgc cattcccaaa ataactgata ttatctttgt
    39721 gtggcacatt tgtctcagtg atcaataccg ttcctggagc tgcttcatca gcgattgcac
    39781 ggaataattg gatgatttta tgcgtttctg gcaggtgtat acaagaagtt cctagttctt
    39841 tccataaaaa tcccacagcg tccaagcgaa taaatgaagc gcctttttct aagtaaaaca
    39901 aaagaatatc gatcattttc aataaaactt ctggattttt aaaattcaag tctacttgat
    39961 cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg aacgttgtaa
    40021 gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt ggcaaatcaa
    40081 taaaaaaatc tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa gtactttcgg
    40141 cagacatatg attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc atttcttcta
    40201 tatcttccca tgtgcccaaa cggtcgttga ttgcctgata atttacaacg gagaaaccat
    40261 catcagatgt atatgggaaa aatggcaaga aatgtacaac tctaaatgta tctgccaaat
    40321 acttttgata aaatttctta aatgtacgca aagttggttg ctgctcttca aaaaattgat
    40381 ccccataagt aatcaagaaa atatcttctt cgtcccaatg cagtttgcct gcctggcctt
    40441 tcttctgctg ataaacactg actttttgtt caatcataga ggcggttttc tcagacaaat
    40501 ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat cggattcccc tttctttttt
    40561 gggagcgttc ccaacgattt gatcatatta tggaaaacgc ttactgtcaa tagcattttt
    40621 ttaaataatt ttgatataca caaaaataca aataagaaag cttatgtatc aataattaat
    40681 agcttcttaa aaaaatatgg tctctcatga tcatgcaagc tattcctaaa ttttcaaaat
    40741 catagacaaa aaagaatgtc tattgtacta tggaattatt ctaaaacgct cccaaaaagg
    40801 aggtgcatat aaaattgaaa cctactattt atgacattgc gaaaaaagct aatgtctcaa
    40861 aatcaactgt ctccagagta ttaaataacc agccaaatat ctctgataaa gcaaaagcaa
    40921 gtgtacttca agcaatcaaa gaattgaatt atgaaccgag caaaattgca agaggactgt
    40981 caggaaatgg ttttgacgcg attctagcat tatctcatcg tacttctcat acgacctatg
    41041 gcaatccttt tttttcagat gttattcaat ccatttctgc cgtttctgaa caaaataact
    41101 tcgacttgat tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa tgcgcgaaaa
    41161 agatccaaga aaaaatgatc cgaggaatta ttattttaag ctctacgatg gatgaaacac
    41221 ttctttttga attggatcaa tataatattc ctattgtggt aatcggaaaa gtcggaaacg
    41281 actacacgaa catctactca gtggacacgg ataatttcca ggatagttac gatttggtgg
    41341 aaacactgat caagaatgga caccgtcata tcggctgtct ccatgctccg acagaagttt
    41401 atgtatccaa tgatcgtctc aatggttacc gtcaatgctt atttgatcac cacctagata
    41461 tccgtaatga atggattata gacggcggct ttcgcttaga agatagcatg gcagcaatga
    41521 atgatttaat gaagctacga gaaagaccaa ctgcgatttt ttctaccgat gcattaaaat
    41581 cattgagtat ttacaaagtg gcagaacaat ctggaaagca gatacctgat gatctttcga
    41641 tcgcaggatt cagtgatccc ttattgtcct ccctattttc gccacagttg actcggatag
    41701 acattccgac aaatcagtta ggaatcaaag ccacagaact tttattccaa ctaatccacc
    41761 accaataccc tgaagaaaaa agaatcattc tttcaaccga gattactctc acagattcga
    41821 tccaacaaca agaaatgtag aacagaatta taaaagaaca gaaacactga ggataagtca
    41881 gttactctgt tcttttttca atcactcatc cctcgttacc ccctcccggt attttagcga
    41941 atttttctta ttttccatct gttttagata gctgtggata actataatgt ttgtgttaaa
    42001 ctattttttc cacagggaag aaaaaactat ttttaaccac gttgtgtata agtttttata
    42061 gttcgttttt ttaaacacat ttacccttat ttcaatccat gttatttggg gatacttatc
    42121 tctaaaacat aaatagaata acagacatgt ctactggttt tgttttattc gaggaattat
    42181 tcaataatag ctagcaaaat attaaaaata accgctctat cttcccatca atttattttg
    42241 cattcaaaaa atgatctatc tttacttttg ttgattgact tctcttggaa gatgatcgtt
    42301 ccgatacctt aattggatac gaaccgatgt tgttaagatc ctttcccact tttcttctag
    42361 ttagacaaat tattttagac aaaccaaaaa agaggttgtg acaataattt gtcacaacct
    42421 cttttttaaa atatctattt tgtttgattg ttcattgcgt cttctgcagc catttgcgct
    42481 tcaatatctg aaatactgta gacattttcg cttgctacac cgacttcttt tggttccatg
    42541 tcacgataac gttgcatacc agtaccagct gggatgatct tacctataat aacattttct
    42601 ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag catccgtcaa gacacgagtt
    42661 gtttcttgga atgatgcagc agataagaaa ctatttgttt ctaatgatgc ttttgtgata
    42721 cctagtaaga caggtcgaga agttgcagga acgcctcctg agactagcgt attgtagttt
    42781 tgatctttaa agtctgcgat gtctaataat gtacctggta agatatctgt atcacctgga
    42841 tccattacac gaactttacg cagcatttga cgaaccatta cttcgatatg cttgtcgccg
    42901 atttctaccc cttgcatacg gtaaacacgt tgtacttcac gaagcaagta gttttcaaca
    42961 gataatacat cgcgaacttg caacaattgt tttggatcga ttgatccttc tgtcaatggt
    43021 gcaccacggt ggatcatatc gccttctgct actttcatac gtgctgtata aggaacagta
    43081 tacgtacgtg tatctgtttt tcctttgatt gttacttctt tggtacgtgt tgcaggatct
    43141 tctgagatat cgatgacatc ccctgttact tctgtgatca ctgcttgccc tttcggatta
    43201 cgtgcttcaa agatttcttg gacacgagga agaccttgag tgatatcgtc tccggcaacc
    43261 ccacctgtat ggaatgtacg catcgtcaac tgagtaccag gttcaccgat tgattgagcg
    43321 gcgatcgtac caactgcttc tccgacttca acgtcagagc cagttgcaag gttgcgtccg
    43381 taacaatgtt tgcagacacc atgttttgta ttacatgtga agacagaacg gattgtcact
    43441 gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa ttggtttcct
    43501 gcaatgatca attcatttgt ttcaggatga cgtacttctt tacgggtgta acgtcccact
    43561 aaacgatctt ctaatgattc gatgatttca tttccctcgc ggatggcttg gatttcaaga
    43621 cctcggtcag taccgcaatc ttcttcacgg atgattacat cttgggccac gtcaaccaga
    43681 cgacgagtca agtaacctga atcggcagtc ttcaaggctg tatcggtcat ccctttacga
    43741 gccccgtgag ttgagataaa catttccaag acggatagtc cttcacggaa gttcgaaatg
    43801 attggcaatt ccatgattcg accattagga gcggccatca aaccacgcat tccggcaagc
    43861 tgtgtaaagt tggaaatgtt accacgagct ccagaatcag acatcatgaa gattggattt
    43921 ttcgcttcca aaccttccat cagtttttgt tggatgctgt cttttgcagc attccaaaca
    43981 gcaatgacac gttcataacg ttcgtcatct gtgatcagac cacgacggaa ttgttttgta
    44041 atagtttcaa cttgtttatg ggcattttca atgatttctt gtttttcatt cagtacactg
    44101 atgtcagcga tccctactgt gataccggca tgtgtcgaat gtttgtaccc taagtctttc
    44161 atacggtcaa gcatcttaga agtttctgtt actttgaatc gtttgaagac ttctgcgatg
    44221 atgtttccta agtttttctt cttgaacggt aatactaatt cttgctcttt gatatgtgca
    44281 ggaatatcag taccagcttc aacaaagtat ttgtccggtg tttggacggt caagttgaag
    44341 tctgttggtt cattcagata agggaattca ggaggcatga tctcgttgaa gatgatctta
    44401 ccaactgttg tgatcatcgt acgttctttt tgccattcag taaatggttt ttcacctaat
    44461 gagttagggt tcacaccgat acgtgaatgt aaatgaacat atccgttgcg ccatgcgata
    44521 acggcttcgt tttgatctct gaagaccatt ccttcgcctt cgcgtccttc ttcttccatt
    44581 gtcaagtagt agttacctag aaccatatct tgagaaggag taacaactgg tttaccatct
    44641 ttagggttca agatgttttg agcagctaac atcaacatac gtgcttctgc ttgtgcttct
    44701 tcattcaatg gaacgtggac agccatttgg tctccatcga aatcggcatt ataagcttca
    44761 catacaagtg gatgcaaacg gattgcgcgt ccttgtacta aaactggttc gaatgcttgg
    44821 ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc cttgattacg
    44881 tcttctaaaa catcccatac ttcgtcttct tgacgttcga ttttacgttt cgcatttttg
    44941 atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt gaataattca
    45001 atggccattt cttttggaag tccacattgg tacatcttca agaaaggtcc aacaacgata
    45061 acggaacgac ctgagtagtc gacacgttta cctagcaagt tttgacggaa acgaccttgt
    45121 ttccctttca acatatgaga aagagatttc aatgggcggt tacctggtcc agtaactgga
    45181 cgtccgcgac gaccgttatc gatcaatgca tctactgctt cttgcagcat acgtttttca
    45241 ttttgaacga tgattccagg tgcattcaag tctaacaaac gtttcaaacg gttattacgg
    45301 ttgattacac gacggtaaag atcattcaag tcacttgttg caaaacgtcc accttctaat
    45361 tgaaccattg ggcgtaaatc tggtggaata acagggataa cgtccataac catccagctt
    45421 ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca aacggcggat tgcacgtgta
    45481 cgtttttgtc cttgtgcaga tttcaattct tctttcaatt cggctgcttc tgcatctaga
    45541 tcaacattat caagtaattg tttaatcgct tctgcaccca tagcagcttg gaattcttgt
    45601 ccatattgtt cacgtttttc gcgatattcg cgttctgtca acaattgttt tttctctaaa
    45661 ttcgtatttc ctggttcgat gactacatat gaagcaaaat aaatgatttc ttccaaagcg
    45721 cgtgggctca tgtctaatac aagacccata cgtgaaggaa tccctttaaa gtaccagata
    45781 tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac tttcgaacga
    45841 gtaacttcca ctccgcatcg atcacaaacg attcctttat aacggatacg tttgtatttt
    45901 ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc gctcacagaa caacccttca
    45961 cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt tttttacttc accgtaagac
    46021 cagcttctga ttttttcggg agaagccaaa cctatttgca tactttcgaa tttatttaca
    46081 tcgatcaaaa ggggttccct ccatttcatt taatggcaaa ccgcaagacg gctgatcttc
    46141 gaccgccttg cagctcatag accggattag tctaaattat cttctgcggt ttcaaagttt
    46201 tggatgacat cgtctttttc atcttccact tgttcagcag ctttcttttc taattctttg
    46261 gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc gtcgtcttca
    46321 tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat ccaagacacg catgtccaga
    46381 cctagtgatt gcaattcttt caccaatacg cggaatgatt ccggtacacc tggttttgga
    46441 attggttcac ccttgacgat tgcttcgtat gttttcacac gtccgactac gtcatctgat
    46501 ttgtatgtca agatttcttg taaagtataa gccgcaccgt aagcttccag tgcccatact
    46561 tccatttcac caaaacgttg tccaccaaat tgagctttac ctcccaatgg ttgttgtgta
    46621 accaatgagt aaggtccgat tgaacgtgca tgcaatttgt catcgaccat gtgagcaagt
    46681 ttgatcatgt acatcacacc gacagacaca cgtccatcaa atggttcacc tgtacgtccg
    46741 tcgtacagaa cagttttcgc atcgctggcc ataccggctt cacgaactgt ttcccatacg
    46801 tcttcatcac ttgcaccatc aaatactggc gttgctacgt ggatacctaa ctgacgtgca
    46861 gccataccca agtgtaattc caatacttgc ccgatgttca tacgtgaagg cacccctaaa
    46921 ggattcagca tgatatcgat tggtgttcca tcaggtagga atggcatatc ttcttccggc
    46981 ataatacggg atacaacccc tttatttccg tgacgaccgg ccattttatc cccttcatgg
    47041 attttacgtt tttgaacgat ataaacacga actaacatgt ttacgcctgg tgacaattca
    47101 tctccagctt cacgagtaaa gattttcaca tcgtgaacga taccgccgcc gccatgtggt
    47161 acacgtaatg atgtatcgcg gacttcacga gctttttcgc caaagatcgc atgcaataga
    47221 cgttcttctg cagataattc tgttacccct tttggcgtga ctttccctac aagcaaatcg
    47281 ccatcttgga cttctgcacc aatacggatg atacccattt cgtctaaatc ttttaatgcg
    47341 tcttccccga cattagggat ttcgcgtgtg atttcttcag gtcctaattt tgtatcgcgg
    47401 gcttctgatt catattcttc aatatggata gaagtgtata cgtcatcttt taccaaacga
    47461 cggctcatga tgatcgcatc ttcatagttg tacccttccc aagtcatgaa accaactaaa
    47521 acgttttggc ctagagccat ttcgccttgt tccatagatg gtccgtctgc cagagtatcc
    47581 cctttttcga ctttctcacc caaatgaacg attggacgtt ggttgtagct tgttccggag
    47641 tttgaacgac ggaattttgt tacatcgtat ttatctaatg caccgttgtc gcggcgtacg
    47701 cggatttcag aagcatcaac gtattcaacg acaccgtcat gtttacataa caaagcagct
    47761 cctgagtcat gcgctgattt gtattccata ccagtaccaa cccatggtgc ttgaggattg
    47821 atcaacggca ctgcttgacg ctgcatgttg gcacccatca aggcacggtt ggagtcatcg
    47881 ttttccaaga aaggaataca tgctgtcgca acggcaacta cttgtttagg tgatacgtcc
    47941 atgtagtcta ctttatcaat agatacttct aagttttcac tttgagcacg agccataaca
    48001 acgtcttgag caaatgttcc atcttcattc aatgggctgt ttgcttgggc tacgatgtaa
    48061 tggtcttcga tatcagctgt caagtagtcg atttggtcag taacacgacc tgtttcacgg
    48121 tctacacgac gataaggtgt ttcgataaat ccgaatttat ttactttcgc ataactggac
    48181 aaactattga tcaacccgat gtttgggcct tcaggagttt cgatcggaca catacggcca
    48241 tagtgggagt agtgaacgtc acgaacttca taaccggcgc ggtcacgagt caatccgcca
    48301 ggtcctaagg ctgaaagacg acgtttgtgt gtcaattcgc caagagggtt tgtttgatcc
    48361 atgaactgtg acaactgaga agaaccaaag aattctttga tacttgccac aactggacgg
    48421 atattgatca attgttgagg tgtcaatgtt tcagtatctt ggatcgacat tctttcacga
    48481 accacacgtt ccatacgtgc tagcccgata cggaattggt tttgtaaaag ttcaccgact
    48541 gaacggatac gacgatttcc taagtggtcg atatcatcga cattgccgat accttccatc
    48601 aagttcaaga aatagctcat tgaagcaatg atgtcggctg gacgaaccgt ttttacagct
    48661 gcttctggat agccgtttcc gatcacgttt acttcacgtt cagggtctct tggtgaaaat
    48721 actttgatga cttggacagt cattggatct gtgaccacac cgtcttctga tggatagtaa
    48781 gtcactgaat ttaggccatt atcaatgaaa ggagctagtg tttccatgac ttggtgagtc
    48841 agaactgttc ctttttcaac gatgatctcg cctgtttctg gatcaactaa tgtttcagct
    48901 aatgtcagat tcaatagacg tgttttcaaa tctaattttt tgtttacttt gtaacgacca
    48961 acgtttgcta agtcgtaacg ttttggatcg aagaaacgag cattcagcaa gttacgtgag
    49021 ctgtctgctg tttttggttc gcctggacgc aaacgttcgt aaacgtcttt caaaccttct
    49081 tctgttcttg agtcgcttgc gtttttatgc aagtcttttt cgactgtatt gcgtaatgtt
    49141 tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac caaatcctaa cgcacgaact
    49201 aatacggtta aaggaatctt acgtgtacgg tcgatacgta cataagagat gtcttttgca
    49261 tcagtttcca tttctagcca agctccacgg ttagggataa cagtagaacc gaatccttct
    49321 tttccgtttt tgtcgacttt tccatggaaa tagacacctg gtgaacggac taactgagaa
    49381 acgattactc gctctgcccc gttgatgatg aatgttcctt gttctgtcat taatgggaaa
    49441 tcaccgaaga atacttcttg tgctttgatt tcgccagtct cgcggtttgt caaacgcaat
    49501 gttacatgta atggtgcaga gtagtttgca tcatgcgcac gagcttctgc tactgtatac
    49561 tttggttctt tcaattcata atctacaaat tccaaagata gattgccatt gaagtcatcg
    49621 attggcaaaa tgtcttcaaa catttctcta agtccctcat ctaagaacca ttggtaagag
    49681 tctgtttgaa tttcgatcaa atttggtaat tccaacactt cactgattct tgcgaaactt
    49741 ctacgttcgc gatgttttcc gtattttact acgtgtccag ccaagctctt cacccctcta
    49801 aaatgtcatt caaaaagact acccgatcaa ttgttacaat aatatttcaa atattatttt
    49861 taggtaacaa cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa atagaacatt
    49921 ctcttgaaac cttacttcgt gagcttctac ttttgtttcc ttatattaaa gccgaaacgg
    49981 acaatatttc gtttctgcct tttcttcatc agatagtttt tgcaacttca tatactacac
    50041 ttattttcta taaaagtcaa cacttcctgg cgtttcttct gctttttcgc aacattttct
    50101 taatgttttc aattttatgt tttcaattat ctgacaattc ttaattgaca gaccttttta
    50161 tctcggttat actttagaaa attcattttg tggaggaact tctatgcgaa ccaacatcaa
    50221 ccaaatcgca ccaacacaac tgaataactt ttactttagc tactttagat aggtttgtat
    50281 tcatcatgat ggatacaaac gttaaacgac agtttgtatc tatgatggct aaagaacttt
    50341 gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg ctgagcagtg aaaatacaaa
    50401 gtaaaagtat caagcattct actttttcaa tttattttcc gacttttcaa tccatttgga
    50461 cattttgtct gaatggattt tttgtttccc aaaattaagg aggaaaaaga aatgaaaaaa
    50521 aatataatca tcggattagg cgctgccctg ctactagctt taggaggttg cgcacaaaaa
    50581 gacaattccg cagaggcatc agatggacaa aaaacaattg gcgtattgca acttgtacaa
    50641 cataactcgt tagatgctgc ctataaagga ttcaaagaag gattagcaga agaagggtat
    50701 aaagaaggcg ataatctaac aatcgattat caaaatgccc aaaacaatca agacaacctg
    50761 aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt tgctacttgg aatcgccact
    50821 ccagcagcac aatctttatt aaatgaaaca accactatcc cgatgactgt cacagcggtc
    50881 acagacttgg aacaagccca tttagtcaca tccaatgaaa aacctggcgg caatgtgact
    50941 ggcacgacag atatggtgcc aatcgaaaaa caaatcggct tactgttaaa tatcgtgcca
    51001 gacgccaaaa caatcggcat catgtataac aacggtgaag caaactctaa gatccaagct
    51061 gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag tcaaagtact gacagcaaat
    51121 tccacaaatg atgtccaaca agtgacgaaa agtctagcta aagatgtaga tggaatctat
    51181 attcctactg ataacacgtt cgcaagtgcg gcaagtgttg tcggtgaagt cgcaaaagaa
    51241 acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca aagacggcgg tctagcaaca
    51301 gtaggaatcg actacgaaag cttgggcaaa caaacaggga tcatggcagc aaaaatctta
    51361 gacggcaaga atccaagtga acttcctgtc gaaacagcga atgatttgga actgtatgtc
    51421 aataaagaca tggccaaagc attaggcatc aatccagaca gcatcaaaga agctgaataa
    51481 aacacaggag gattctcatg acaaatttgc tctctctttt cttatcatct gcatcgcaag
    51541 gggtcttatg ggccctactt gcgattggtg tatacgtcac tttccgtatc ttggacattg
    51601 ccgacttgac tgctgaagga agctttcctt taggtgcagg gatcgcggca gtaagtatta
    51661 caaatggcta tcatccattg acagcttgtc ttctaggctt tttagggggt gcggcagcag
    51721 ggcttgtttc cggactgctt cacacaaaat tgaaaattcc cgcccttctt gcagggatca
    51781 tcaccatgac tggtttatat tctgtcacat cccgtgtgat gggtgcgccg aacatctctt
    51841 tattgggtca gccaacgatc tttcattggg tcgagtcatt cggcataaca aaagacaacg
    51901 ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt atgtttactt gttttgttct
    51961 tacgtacaga aacaggactt gctattcgtg caactggcga taatctggca atgagcgaag
    52021 caaacgggat caacacagat aatatgaaga tcatcggtta tatgatttct aatggatgca
    52081 ttgctctttc tggctcctta cttgcgcaaa acaacggctt tgctgattta aattctggta
    52141 ttggaacgat cgtaattgga cttgcttcta ttatcatcgc agaagttttg ttacgtaacc
    52201 agccactatt tctccgtctc ttgactgtcg ttttaggtgc ggtcatttat cgttttattt
    52261 tagcgttagt cttcgaactg aatgttgaac caagtgattc gaaacttgct tctgctttcg
    52321 tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa attccattta cctcaaaaaa
    52381 agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg gaaaatctcc atcatacctt
    52441 tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc attaatcttt ccttagatcc
    52501 cggcgacttc gtgacgatca tcggtggaaa tggggctggt aaatctactt tgctgaacag
    52561 catcgccggt acattttctg tcgatcaggg tcaaatttta ttgaatggaa aaaatatcac
    52621 gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc gtattccaag atcctaaact
    52681 agggacagcc gttcgtttaa cggtagaaga aaatcttgct cttgctatga aacgaggaaa
    52741 aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga acatttttca aagaccagct
    52801 ggttcggcta aatttggggt tggaaaatcg tttatcctca gaaatcggtt tgctgtctgg
    52861 aggacaacgt caagctatta cgttattgat ggccacatta cagcgtccag aactgatctt
    52921 attagatgaa cacactgcag cgcttgaccc tgctacttcc aaaacggtga tggaattgac
    52981 agaaaagctg atcactgagc agaatctcac tgcttttatg gtcactcatg acatggaaga
    53041 cgcgatccgc tatgggagtc gactgatcat gctgcatcaa ggaaggatcg tcgtagatat
    53101 ccagcaagag gaaaaacagc agctcacagt gccccaatta ttagcgttgt tcaaacaaaa
    53161 cagcggcacg gcattaaaag acgatcaagt acttttatct tcataatcag aaagaggatg
    53221 ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt ccttctttcg tttttgcttt
    53281 atgtatcaac cgttgtttct tcgaaaacta ttttgttctc tccttcttcc tgatagaatg
    53341 gatgggaaag gagcttgatg ccatgacaac gaaagacaag gtacttcaat tattgaaaaa
    53401 gacaccagat tttctttccg gagaaaagtt ggcacagcaa ctggaagttt cccggacaag
    53461 tatttggaaa gccatcaaag aattggaaaa ggcaggctat cgttttgagc acgggcctac
    53521 agggtatcgt tacctgccct ctgacgtact agatgcaaaa gaaatccagc aagagctacg
    53581 gtcgtttatt cctgatttga aagtacaaac gatgttgact tctgaatcta cgatgaaaga
    53641 tgccaaactg gcagcagctc aaggagattc tgtttcgaag ttatttgttg ctgatacaca
    53701 agaacaagct cgtggacgtt ttggacggcc ttttttcgct gaacctggac gaggaatcta
    53761 catgagtctc ttgctccatc cagacaaacg gtttgatgaa ttgcctcaat atacgatcat
    53821 cgcagccagt gcctgtgtga aagccattga atctgtaacc ggtcgttctg cttcgatcaa
    53881 atgggtcaat gatatctaca tggatggaaa aaaagtctgt gggattttat ctgaagcagt
    53941 cagtgatatg gaaagcggca ggatcagtca tatcatcatt gggatcgggc ttaatttttc
    54001 tgttcctcaa aatcattttc cagctgatct ccaagaaaaa gccatctctg tctttcccga
    54061 cggacaagct agtgtcacaa ggaatcagct aatcagcaaa atctggcaag aattgttcac
    54121 ttatctttct cacccagaaa aagcgttgga cttctataaa gaaaaatcat tcgtattggg
    54181 aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc ggtcttgcaa aagaaatcgg
    54241 tgatcatggc gaactgatcg tccaaacaaa tgaacagaag atgagtttgt cttctggcga
    54301 aatcagtctc acatctatcc gtacgcaata attatcagat ttcacaaaag gacacctgat
    54361 ctcatacagt gtcctttttt ctgaattctc ttcaaaagac tctccttatt ctatcagctg
    54421 acagtttctc tatcctctag aaaaaggtat tctaaaaata tctataaagc atggtacact
    54481 ttctttaaat gaatcaatgc aaaaaacaag agagtcatca gtttacacga tcaaaagaga
    54541 tagcaggccg cttccttttt caatcttata gatagaccat gggtaacgat aaaaacaaaa
    54601 aagaacttgc gacttatttt gccgtaagtt cttttttagc tagtgcctta atctcttaat
    54661 cccaacaaac gtctcgcata gatcacctga ccagagtgca tagcagcatc atcaataata
    54721 gaaacgagac gcattcctcg tgtgactggt ggtgtccagt tctcgtcaat tacttcatct
    54781 agctcggaag gttccagcct tcttaagtaa gaaatagcaa aatcagcagc tcctcgcaag
    54841 tatcctagca gatcttctat ttgattagct tggatctgct ttgcttgttc cagactatgc
    54901 ttccaatctt gttcgttatc tgctatcgtc atcggaaacc ggctttccca gtcttgacta
    54961 tgccaaattg gagaagttcc tgctaaatca gcaatctgga aatctaattc tcgagcggta
    55021 tgccaagcca gccaagtcat tgatttgatc tgcggggctc tatcttccac tggaaaacga
    55081 ttggcctcct caatagagac gccttcaaac aaacgttcaa accgatcgta tgcccgatcc
    55141 acactctcgc atagcatatc taaataatcc atttattttc ctccttttta tttatcctac
    55201 ttactttttc ctaattctag gatacctact tatatgttac tacacaccct tattttttac
    55261 actttaacag tcaccatcat caatagtttg gagattgaaa gaaaaagata gtgtcgaact
    55321 atcaattgaa caagaagcaa caatatgaac gtttcattaa tattctttaa ataaacaatg
    55381 atttttcaaa cgaacagttt aagactagga gaaaaagagt ataataggaa aatgtttgaa
    55441 aggaaaggaa ggggaaaaat gaaaaaaagg gcaagtttac tagtcctagt tgctttgttg
    55501 ttcaatatct tttcgtatgg tgtggccgtt tcagcggaaa catacattcc taaagaccct
    55561 gggaatattt ataatgatct aactggtaac tatggcttgt taggaattgc tagtcaattc
    55621 cacgtatttg ctaaaaataa gacaactgtg aatgcacata cagacggaaa tatagcaaca
    55681 catgaacttg atgcaaaaaa taattttggt acagagcttc atgaaggttt attgaaccaa
    55741 gaaataaatt atatacaaaa aactgattct ttcgctaatt cttcaggcat acctacaggt
    55801 agcacaagaa tgaataaatt tgtcgttggc agtaacgtgg atgtagcact tcaagatggt
    55861 catcctacag tcaatggatc caggcttgat cacttaactc cagatgattt ttataaagat
    55921 cgtgctgggc atgaatatat tgatttcgag caggaatttg ctaaattgaa tgtagcagca
    55981 aatgatcttg caacgatcac tccagctaaa acatatactg ctgcagattt tccagacatg
    56041 aacaatcgga caatcgattt aactgattta tcagataccg ggtttttatt ggtaaatatc
    56101 gatgcagaag tgttaacgat gaatacgcca cttcaaatca ttaatccaaa tgatcaagta
    56161 gttgtattta atgtcatcaa cagttcttct gcattgaacg tacagtcacc aatcaaatac
    56221 aatgatcgtt caaatcatga aacagaagat ttttcggatg cgaatatctc ttggaatttt
    56281 ggaaatgaga tgactgattt gacaatcagt gctccttttc aaggaacaat ccttgcgcct
    56341 aatgctacta tccgcattac tcaaaaccaa gacggaacga ttataggaac aaatgtgatt
    56401 ttagatgcag ctaccaatcg ttgggatcct aacgaaatat ttatcagcga caatggtaca
    56461 gatacgacag acacaagcga cagtacagat acatctacca caagtgatag ctcagattca
    56521 tcaacttcaa gcgacagtac agatactacc tctaccacaa gtgatagcac ggatac
//
LOCUS       DARBEG010000009        51636 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000009 DARBEG010000000
VERSION     DARBEG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.009"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            120..1046
                     /gene="cysK"
                     /locus_tag="SCK20_000531"
     CDS             120..1046
                     /gene="cysK"
                     /locus_tag="SCK20_000531"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG1144624.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1282..1728
                     /locus_tag="SCK20_000532"
     CDS             1282..1728
                     /locus_tag="SCK20_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1144625.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2001..3353
                     /locus_tag="SCK20_000533"
     CDS             2001..3353
                     /locus_tag="SCK20_000533"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG1144626.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3524..3811
                     /locus_tag="SCK20_000534"
     CDS             3524..3811
                     /locus_tag="SCK20_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144627.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4037..4480
                     /locus_tag="SCK20_000535"
     CDS             4037..4480
                     /locus_tag="SCK20_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1144628.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4486..5670)
                     /locus_tag="SCK20_000536"
     CDS             complement(4486..5670)
                     /locus_tag="SCK20_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG1144629.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5938..6636
                     /locus_tag="SCK20_000537"
     CDS             5938..6636
                     /locus_tag="SCK20_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1144630.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6643..7320
                     /gene="nth"
                     /locus_tag="SCK20_000538"
     CDS             6643..7320
                     /gene="nth"
                     /locus_tag="SCK20_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG1144631.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7446..7979)
                     /locus_tag="SCK20_000539"
     CDS             complement(7446..7979)
                     /locus_tag="SCK20_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144632.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8276..10657)
                     /locus_tag="SCK20_000540"
     CDS             complement(8276..10657)
                     /locus_tag="SCK20_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1144633.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10647..11294)
                     /gene="recU"
                     /locus_tag="SCK20_000541"
     CDS             complement(10647..11294)
                     /gene="recU"
                     /locus_tag="SCK20_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG1144634.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11358..11897
                     /locus_tag="SCK20_000542"
     CDS             11358..11897
                     /locus_tag="SCK20_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG1144635.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11987..12406
                     /gene="gpsB"
                     /locus_tag="SCK20_000543"
     CDS             11987..12406
                     /gene="gpsB"
                     /locus_tag="SCK20_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG1144636.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12428..12795
                     /gene="rnpB"
                     /locus_tag="SCK20_000544"
     ncRNA           12428..12795
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SCK20_000544"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13004..14170
                     /locus_tag="SCK20_000545"
     CDS             13004..14170
                     /locus_tag="SCK20_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG1144637.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14216..15712
                     /locus_tag="SCK20_000546"
     CDS             14216..15712
                     /locus_tag="SCK20_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG1144638.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15793..16476
                     /locus_tag="SCK20_000547"
     CDS             15793..16476
                     /locus_tag="SCK20_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1144639.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17121..18311
                     /locus_tag="SCK20_000548"
     CDS             17121..18311
                     /locus_tag="SCK20_000548"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG1144640.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18413..19366
                     /locus_tag="SCK20_000549"
     CDS             18413..19366
                     /locus_tag="SCK20_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG1144641.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            19435..20784
                     /locus_tag="SCK20_000550"
     CDS             19435..20784
                     /locus_tag="SCK20_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1144642.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20967..21290)
                     /locus_tag="SCK20_000551"
     CDS             complement(20967..21290)
                     /locus_tag="SCK20_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144643.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21419..22582
                     /locus_tag="SCK20_000552"
     CDS             21419..22582
                     /locus_tag="SCK20_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1144644.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22652..24001
                     /locus_tag="SCK20_000553"
     CDS             22652..24001
                     /locus_tag="SCK20_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1144645.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24046..24477)
                     /locus_tag="SCK20_000554"
     CDS             complement(24046..24477)
                     /locus_tag="SCK20_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1144646.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            24865..27507
                     /gene="alaS"
                     /locus_tag="SCK20_000555"
     CDS             24865..27507
                     /gene="alaS"
                     /locus_tag="SCK20_000555"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG1144647.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27707..28303
                     /locus_tag="SCK20_000556"
     CDS             27707..28303
                     /locus_tag="SCK20_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1144648.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28425..29573
                     /locus_tag="SCK20_000557"
     CDS             28425..29573
                     /locus_tag="SCK20_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1144649.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29611..30024)
                     /locus_tag="SCK20_000558"
     CDS             complement(29611..30024)
                     /locus_tag="SCK20_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG1144650.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30103..30534
                     /locus_tag="SCK20_000559"
     CDS             30103..30534
                     /locus_tag="SCK20_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG1144651.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30633..30866)
                     /locus_tag="SCK20_000560"
     CDS             complement(30633..30866)
                     /locus_tag="SCK20_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG1144652.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31049..32719
                     /locus_tag="SCK20_000561"
     CDS             31049..32719
                     /locus_tag="SCK20_000561"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG1144653.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            32866..33252
                     /locus_tag="SCK20_000562"
     CDS             32866..33252
                     /locus_tag="SCK20_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144654.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            33239..33937
                     /locus_tag="SCK20_000563"
     CDS             33239..33937
                     /locus_tag="SCK20_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1144655.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            33942..34406
                     /locus_tag="SCK20_000564"
     CDS             33942..34406
                     /locus_tag="SCK20_000564"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG1144656.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            34403..35314
                     /locus_tag="SCK20_000565"
     CDS             34403..35314
                     /locus_tag="SCK20_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1144657.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            35549..36088
                     /gene="pyrR"
                     /locus_tag="SCK20_000566"
     CDS             35549..36088
                     /gene="pyrR"
                     /locus_tag="SCK20_000566"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG1144658.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36102..37385
                     /locus_tag="SCK20_000567"
     CDS             36102..37385
                     /locus_tag="SCK20_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG1144659.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            37398..38324
                     /locus_tag="SCK20_000568"
     CDS             37398..38324
                     /locus_tag="SCK20_000568"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG1144660.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            38343..39623
                     /locus_tag="SCK20_000569"
     CDS             38343..39623
                     /locus_tag="SCK20_000569"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG1144661.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            39628..40704
                     /gene="carA"
                     /locus_tag="SCK20_000570"
     CDS             39628..40704
                     /gene="carA"
                     /locus_tag="SCK20_000570"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG1144662.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            40709..43894
                     /gene="carB"
                     /locus_tag="SCK20_000571"
     CDS             40709..43894
                     /gene="carB"
                     /locus_tag="SCK20_000571"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG1144663.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            43948..44724
                     /locus_tag="SCK20_000572"
     CDS             43948..44724
                     /locus_tag="SCK20_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG1144664.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            44721..45653
                     /locus_tag="SCK20_000573"
     CDS             44721..45653
                     /locus_tag="SCK20_000573"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG1144665.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            45650..46366
                     /gene="pyrF"
                     /locus_tag="SCK20_000574"
     CDS             45650..46366
                     /gene="pyrF"
                     /locus_tag="SCK20_000574"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG1144666.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            46373..47062
                     /locus_tag="SCK20_000575"
     CDS             46373..47062
                     /locus_tag="SCK20_000575"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG1144667.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            47035..47304
                     /locus_tag="SCK20_000576"
     CDS             47035..47304
                     /locus_tag="SCK20_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144668.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            47342..47983
                     /locus_tag="SCK20_000577"
     CDS             47342..47983
                     /locus_tag="SCK20_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1144669.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48026..48718
                     /locus_tag="SCK20_000578"
     CDS             48026..48718
                     /locus_tag="SCK20_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG1144670.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            48715..49617
                     /locus_tag="SCK20_000579"
     CDS             48715..49617
                     /locus_tag="SCK20_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1144671.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(49775..51481)
                     /gene="efbA"
                     /locus_tag="SCK20_000580"
     CDS             complement(49775..51481)
                     /gene="efbA"
                     /locus_tag="SCK20_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG1144672.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        16895 a         8983 c        11096 g        14662 t
ORIGIN      
        1 aattaccata tatggtatat ctttgtcttt tttttgctag tcaaagacga atcactcgcc
       61 ataaatgaac agattgtatt atgataagta agaagagcaa agacaaggag gaattgaaga
      121 tgacagctat ctataactca gtcaccgaat tgattggcaa aacgccaatc gtcaaattaa
      181 ataaaatcgt accagaagat tcagcagatg tttttgtaaa gcttgagttt tttaatccag
      241 gaggaagtgt aaaagatcgt attgctttaa gtatgataga aaaagcagaa cacgatggtc
      301 tactgaaacc aggtgatact atcattgaac ctacttctgg aaatacagga atcggattgt
      361 cgatggtagg agttgcaaaa ggatataaag taatcatcgt gatgcctgag acgatgagta
      421 tcgaaagacg tttattgatg aaagggtacg gcgcagagtt gattttgact cctggtgcgg
      481 atggtatttc tggatcaatc agagaagcag aacgtttggc aaaagaaaac ggctactttt
      541 taccattaca atttgaaaat gaagcaaatc ctcttgtgca tgagaagacg actggtcctg
      601 agatacatca agcatttgga gtaaatggct tggacgcgtt tgttgcaggt atcggtacag
      661 gaggaacgat cactggcgct ggccgagaat taaaacgtgt gtatccgaaa atcgaattaa
      721 tcggggtaga accagcagaa tctgctattt tagaagggaa agaagcggga cctcacaaaa
      781 tccaaggaat cggtacaggt tttgttccta aaacactgga tacttctgtg tatgataaag
      841 tcctttctat tagtggagac gaagcgatgg aaacagctag agaagttggg cgaaaagaag
      901 ggattttagt aggcatctca tctggcgctg caattgctgc cgcgctaaaa gtcgcaaaag
      961 aattaggaaa aggcaagaag gtattagctg tggttcccga caatggtgaa cgttatttat
     1021 ctacagccct ttaccaagag atataaggca cttggttttt ctccagatag gtttatgtaa
     1081 aaaactttta aagatgaaaa gaacacacaa aaatttatga tgagaaaaat tatttgaata
     1141 gagacgtaga atagtgaacg cgactgtttt acgtcttttt tcattaaaat acaataaaat
     1201 tgaattttct tttcatgtta agtggatttt aaaatgtaaa aatagtatga taagacgaga
     1261 ataacaacgt ggggtgttta catggaaaat aagttagtcg aagaggttat cgaaaagtta
     1321 aaaaaagaga acattcgaat cacacctcaa cggtacgcgg ttttagaata tttgattgaa
     1381 catcattccc atccaacggc tgatgagatt tataaagcac ttgaacatcg gtttccaaat
     1441 atgagtgttg cgactgttta caataacctt cgcttattta cttcgattgg atttgttcaa
     1501 gaaatgaagt acggcgatgc ttctagccga tttgatttca gttcaaagcg tcattatcat
     1561 gtaatttgtg aaaactgcgg taagattgtt gattttcatt acccagggct tgaagatata
     1621 gaaatggctg caagcagact gactgatttt gagattaatg aacatcggtt ggaattgtat
     1681 ggactctgtc cggattgtaa agagtcagca aaaaaggcgc aaatgtaaaa acagtttcta
     1741 caagtgttat acctaaaaaa agctagctgt gtaaaaaaaa cgctagcttt tttatttttt
     1801 tgtcttttat tcacaatctt agatataaaa gtgaatatta ttaaaataag aatcttgtag
     1861 atattgggtt cttatgcttg aaaaggaatg aaactacaaa caaacctttg tgaattcaca
     1921 agcattaaac tgttgtatcc gttttatttc tgttatagta gttttgtcga aggacaaaat
     1981 gttaatcgga gggaccataa atgaaagtag tagtaattgg ctgtactcat gcaggtactg
     2041 cagctgtaaa aagtatttta aaaaatcatc cagaagcaga agtgacagta tatgaaagaa
     2101 atgacaatat ttctttctta tcttgcggta tcgcacttta tgtcggcgga gtagtaaagg
     2161 acccagcagg cttgttctac tcaaatcctg aagaattatc ttcattagga gcaacagtta
     2221 aaatggaaca cgatgtaaca gacgtcgata cagaaaataa aacagtaacg gcaaaagatc
     2281 tgaaaacagg agaaacagaa acggtttctt atgataaatt agtgatgaca actggttcat
     2341 ggcctattat cccaccgatt agtggaatcg atgcgaaaaa tgtactccta tgcaaaaact
     2401 acaatcaagc aaatgaaatt attgcacaag cgaaagaagc aaaacgtgta gtaattgttg
     2461 gtggtggata tatcggtatt gaattagtag aagcttttgt cgaatctgga aaacaagtga
     2521 cattgattga tggattagat cgcatcttga ataaatatct agataaacca ttcacagata
     2581 tcttagaaaa agaattgaca gaccgcggtg ttactcttgc gctaggcgaa aatgttactg
     2641 aatttatcac agatgaagaa ggaaaagtga aacaagtagc tacaccaaca gatacatttg
     2701 atacagatat ggtcatttta tgtgtgggct tccgtccaaa tacaaaactg atcgaaggaa
     2761 aagtagatac actgccaaac ggggcaatca aagtgaatga atacatgcag acaagtaatc
     2821 cagacatttt tgcagctgga gactctgcag tagtcaacta caatccaagt ggcacgcaaa
     2881 actatattcc tttggcaaca aatgccgttc gtcaaggttt gcttgtggga aataacttga
     2941 cagatcataa aatggcgtat cgtggtactc aaggaacttc aggactttac ctatttggct
     3001 ggacgatcgg ttcgacaggt gtgaccaaag aaagcgcaac tttgaacgat ctagaagtcc
     3061 aagcgactgt atttgaagat aattatcgtc cagagttcat gccaactact gaaaaagtaa
     3121 tgatggagct agtctatgaa aaaggaacga atcgtatcgt aggtgctcag ttcatgtcca
     3181 aatacgacat tacacaatca gcaaatacca tgtctttagc tgttcaaaat aaaatgactg
     3241 tagaagatct cgcactttca gatttcttct tccaaccaca ttttgaccgc ccatggaatt
     3301 acttgaacct acttgcacaa gcagcgctag gcgaaatcga aactgcacaa taaagaaaaa
     3361 gggttgtgac acaattttgt cacgaccctc tgtctttttt tgtgaaaagt acaattagtt
     3421 gtctttcttc gaattttttg tcacaataag cagcaaagat aaagctgtta cataaaagaa
     3481 caagcagctt tatttgattt tgaaaagaaa ggtgagaaag aagatgaata aagaatattt
     3541 tgtccaacaa gatgcatttt tagaagatta taaaagtgct tcgaagaaat taacgatcaa
     3601 tattgatgag ttggcagatc aagtactctc tcagttgaat gaaacgcaag aggaatatta
     3661 cacgatgcca gcaaatgacg ccaaagataa tgtttctcat cgttttcctt tcagtagacg
     3721 catctactca gatgaaaatg acggcacatt gaatacagag tacacatatg aaggtcagcc
     3781 atatgtgcta gaaactgatg atgatcttta aagacagttt ttgaaaaggc cgctcatcct
     3841 taagtgatga caaaaaatca tcacaattac cgatgactgt ctctttacta tcttttaatc
     3901 agaaaaaggg gttatgacaa gctttgtcaa cccctttttt tcttggtttc agattgggtt
     3961 ttcctacaaa atatttttat ctttgttgaa taggaaacaa aaaaacaata taatgaaaga
     4021 aaaagaaggt gtagaaatgg ataaaagaca actgctaata gaagaatatc tggatttagc
     4081 taatgagatt attcatcgta ataaaccaga aatggaaaaa gaatttgcag gatttactct
     4141 aaatgaacta gaagtgattg aacatatcgg gaaaatccct gatcctaacg tgacaaaatt
     4201 agcgaatgct agttacatga ctagaggagc catcagtaag ctaacaaaaa aactgatagc
     4261 tagagggatc ataaacactt atcaaagcga agagaataaa aaagaaatct attttcaatt
     4321 gacgccaatc ggacaaaaaa taaggaatag acacgagcaa ctccatcaaa aatgggtaca
     4381 gcgagatgct actgtatttg aaaatatgtc aaaagaagaa tttgacacag tatttcgttt
     4441 tatcggtcga tacagagaat ttatacgaag tagcaaatga agctactatt ttttattgta
     4501 gtgataatag gcaaaaatca taagcaaaaa tccgaatacc gtcggaagaa atggtgctgc
     4561 agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc gatagagatt gtatactttg
     4621 ggtaccgccc attacaagac cttgctggtt atctgagaca gaattggata gttggccatt
     4681 gaatacagga gtaaaaatag aatcaccgaa accataacag ataatcccaa ataaaaacaa
     4741 aatcggcagt agaagaattc cagataatgt gatgaataga taggcgagga tttcactgaa
     4801 catccctatt cttgtgatct gctgttctga aaattttttg agcaatcgag gcataatgaa
     4861 caattgcgag acagtatcca agattccaat caaagagaag cttaaaccaa ccaacccagc
     4921 cttccactga aaggcgtcaa tcgaaaattg agaaaagata gattgaagag agccagctgc
     4981 caaccaaaca gcaaaacctg ctgtcaataa aggaataacg gatgaaatct tgaataattg
     5041 ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa tctactgaac gtttttttat
     5101 tggaaggctt tctagcataa atgtgtagcc ataaacggca tttaagaaag taaagagtgc
     5161 tccaataaat ataggaacag aattccccaa ctcagctaat agacccccta taataggacc
     5221 cagcgttgtt cctataccga ctaaagcacc catccatcca aaaactttgg ttctttcatt
     5281 aggttccgtg atgtcggcaa agtaagcata taaggtggag atttctcctg ccgtcaatcc
     5341 ttcaatgata cgaccaagga acaacatcca tatcgaattt cctaaaccaa aaagcaaata
     5401 tccgatactt gatcctaata aactgataat aaggatcgga tgacgcccaa aatgatcact
     5461 taagctaccg agaatggggg cagataagaa ggcagctaga gcataaacag acatgagcaa
     5521 agtgatatag aaagcttgct gatgtatgga gtgagaataa ggcagcgtta aaaacggaag
     5581 tacaggactg acgatagtaa atcctaatcc agtgaataag acagagacgt atccaaaaac
     5641 tttagaatgt ttatttgtga aactgtacat gtaaaaactc ctttatgcaa taatgttaac
     5701 ttggaaacaa aaatagtgta tagtaatttt gtttccttgt caacattcta aggaacagcg
     5761 aaatctactt aatttattta ttccgaaaag tgaagatacg catcaaaaac atgaacaaac
     5821 aaaaatcaag tagacgacat tgtaaatctg accgatcagt gaagtaaata gaaaagaaac
     5881 ctgttcttgc ttcccatgtt ttttttacgt atactgtgat tgagcggagg agaaatcatg
     5941 ttgaatttag aaaattactt aagcgctgga caaacagtca tatctaatct tttacttaaa
     6001 aattatcata ggatcggtct acaatctgaa gaatttttat ttgttctaca gttgcatatg
     6061 gcacagcttg aaggagatac tttccctgat ttgcagatga ttgctatgga tatgggaatc
     6121 aaacaagatc agatttttca aatactggat cgtctcgtaa caaatggatt tatcaagata
     6181 gaaacgacga ttgtcaacgg taaaaaagca gatcgatata atctttaccc catctatgat
     6241 ctattaggtg agtatttaaa aacacaagaa aaaaaatacg aagaaaaaac acaagaaaaa
     6301 gaaatccagt cagtgtatca attatttgaa caagaatttg gacgcccgct ttcttcgatc
     6361 gagttccagc ggatcggaca atggctagaa gaagaccatt atcagccgga aattttgaag
     6421 ctagcactgc gggaagccgt attaaatcag gcttatagtt ttaattacgt tgaccgcatt
     6481 ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac aagtggaaga agaccaaaaa
     6541 cgaagaaaac agcagcttct tcaaagagaa gtagaacagt cagctcaaca agaagaattg
     6601 ccgaaagtct cactgaaaaa ttggttggag gagtagcgga atatgttaag caaacaaaaa
     6661 acaatggaag ctctagaaac gatgtatgga atgtttcccg aagcacatgg agaactcaag
     6721 cacaataatc catttgagtt attgattgca gtgatcttaa gtgcccaggc gacagatgta
     6781 tcagtgaata aagcaacgcc agatttgttt gcctcttttc cgacacccga tgcgttagca
     6841 gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga ttggtttgta tcgaaataaa
     6901 gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac gtttcgatgg gcaagtgcca
     6961 acgtcaagag aagaattgat gtctcttcct ggtgtaggca gaaagacagc aaatgtcgta
     7021 ttgggggatg cattcgggat accagcaatc gcagtagata cccacgtgga acgtgtctca
     7081 aaacgcctga ggatctgcaa actagatgct acggtcatgg aagtagaaga aacactgatg
     7141 cggaaagtac cacaagaatt atgggtaaag acacatcata cactgatttt ttttggtaga
     7201 tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc ctttgctatc aatatgtcaa
     7261 gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa aaaagaaacc acgcctgtag
     7321 acgtggtttc tttaattgca aataaatgta tgaattatct tgttattatg gagaaaagct
     7381 gtttcgttta tcttttttgc atgaaaaagt aaatgtacat actcagtagc taggatggta
     7441 acttattaca atttattgcg ataaaaataa aagttgttat ttttattggg aaaatacccg
     7501 atgtagaaaa taatgccttc caatatacct ataaaataag tccagtattc caaaggatta
     7561 tataaataag tgacgtatct atctaaaggt gtaataaaaa tgataagtga taataaaaaa
     7621 ataccattta taactaaaca taaactaatg gatttgggcg gtttcaggct catcagaaaa
     7681 atatagattc tggtgaacaa caaccataaa aaagtgagaa tcattgcatg agagcttctt
     7741 tccataaatt ttcctttaag agtagtgctt aaaagtacag aaaaaataat aaatgcaagg
     7801 ataggagcaa ttaaagtaaa tttatttaat aataaacaaa atttttcagc ttttttattc
     7861 ttggaataac gaggtattgt tttcgataaa aaaagatggt gcagacaaat tatgataatt
     7921 aagcatacta tcagataact caaaatgaag ccttctttca aaaaattatt caaaaccata
     7981 tattgtattt tttatttgat aataacaatg ggattattaa gaagtggcac taaataaaag
     8041 actagtgtgt atttagccga tttaattata aacaagtaaa tggataaagt atatgtaatg
     8101 ataaaagaaa aatggatttt ctaattttta agctaaatgt tagatcacac atgtatatgc
     8161 tggatagtct tataaaaaat catgtaatac agcttggtac atgattttta tttgtagctg
     8221 ttttttacat agacacaaaa aagaaaccac gcttgtagac gtggtttctt ttttactatc
     8281 tgtttccaga agctgaggtg ctgctggatg gtgtatggtc atttgcagca tccgctccgc
     8341 tagttgctgg ctcactggaa gaagcaggag gagcagatga agacggtgtc gagctttctt
     8401 cggatgaaga agaaggaacc gttgttgaag acgctggctc gcttgtttct gctgtcgtgc
     8461 tttcttttga ggactcagca gttgattcgc tttgactagt agagctagag ctttgagtcg
     8521 ttgtctctgt tgttgagctt ccaggagtct ggacatagct ggaagatgga atagttgttg
     8581 atggtgtaat tgcattcgat ctagctgtat attgatcttt gtagtacagt tcaccgccca
     8641 cacgaattaa tccgcttggc atctcccagt ctgtatttgt cacgttagct gaaacatact
     8701 gcataagctc gcgataaaca tcagaggcta cgtgtgacga ttctgaagtg acaggtgtca
     8761 tttttttatt gtaacctgtc cacacggaaa tcgaataatt tggcgtatag cccgcaaata
     8821 ggatatctgg atacacccca gaagaaatac caagttttgc atactcgtca tctgtgtaat
     8881 tggatgttcc ggtcttaccg gcttgatata acccagcgat ttgcgcattc gtaccagttc
     8941 cttcagtgat cgtatctttt aaaatgtctg tgatcatata agctgtttct ggagacatgg
     9001 ctgtttttcc atctggctca taagtttctt ctgttccgtc ttggaagacg attttattaa
     9061 catattgagg tttgtaataa gtccctccat tggcgaaagc cgcataggct gctgccattt
     9121 ttaaggaaga agctccgtat tttgtcccat cttgttcttc cgtattacta gagatcgcat
     9181 tcgattgatg gatcgtactg tattcgatcc caagattttt taagaaagaa gccactttat
     9241 ctgaaccgac ttcattgaat aatttgactg ctggaacatt acgggataag taaagtgctt
     9301 ggcgtaaagt aatggtgccc atatattgat tatcccagtt tcctacagga gtcgaagtcc
     9361 cttcatagtt atacggtgcg tcagtaatag tttttccagt agaatatttc aaatattcaa
     9421 aagctggtcc gtaatctgtg acaggtttca tcgtagaacc aaaatcacgg gaagtattca
     9481 ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg acgtccgcca atctgggctt
     9541 ttactttccc ggtattggtg tcgataaggg tggaggcaac ttgcatctcg tcgtctgggt
     9601 aagagacata ctgatccgtg ttgacgatat catatagttt cttttgggcg tccagatcca
     9661 aattcgtata aatctctaaa ccgtctgtgt agacattctt atctgttttt tcttgtactt
     9721 cattgatgac ttctttgaca taattatcga cgatcttagt attgtcatcg gattgagtta
     9781 gttcttgcag accatccgtt accggtacat taacagcttg atcatattct gtttgagaga
     9841 ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg tcttttcttc gcctgatctg
     9901 gataaacata aggatcatag gcggaagggg cttgaggcat cccagcaagc aatgctgttt
     9961 gcggtaaaga aagttctgat aatttcttac caaagtacat ctcagaagct gtctccatcc
    10021 cgtacaagcc gtttgacata tacactttat ttacgtagta ggtcaagatc tcttgtttcg
    10081 attttttttg ttccaatctt acagccatcc acgcttcttg ggccttgcgt ttcagagtct
    10141 gatcctcggc actggtggag aagaaggaaa gtttgatcaa ttgctgtgtc aaagtacttc
    10201 cgccttgcag tccgccagag gtaaagttag agagggcaga accgatgatc cgaatagggt
    10261 caaccccgat gtgtttataa aaacggcgat cttctaccga cacgatcgca tcttctaatg
    10321 ttttcggtaa ttcattcgca gaaatttttt cacgcttttc tgcgcctaaa tcttcgaata
    10381 gttcgccatc ttgtgtatat agtttggaag atacggtagc gtctaatttt ttatccgtca
    10441 attcgggcgc atcttttgcg taataccaga aaagtcctac acctgctaaa aagaggatac
    10501 agaaaaatga gagtattcct aaaaggatct tgataaataa tcctttttta tgtgttccag
    10561 aggactttcc agaacctttg ccagaattct tcttcacact ctttttagga gtaggctgtt
    10621 tctgtcgtct agagcttcta gtttgttcat ttgccattct tgctttctcc ttcaatagat
    10681 aatgttgata aatagaagtc aaccgcgtcc aaataaggaa tccgcggaga aataccagtt
    10741 ttgatctcta ttcctagttc ttcgatcaaa gataaaggta gtgatttgcg tcctttttcc
    10801 ttttgggcgt cccagtaagc aatcaaatga acactattca aataaaaaca gcgcttcaaa
    10861 gacgaaaacc ataacaagac aaagcagacc ccattttgtt cgaggcatgc tttcatatgt
    10921 tcgatttgat gggcatggaa gtttttaaag ggaaaagatg ttttgttctt ggtttctttt
    10981 gcttcaaagt ccaagtaaaa gcctcgatag acgccattat agtctgtagt tgaggcttgg
    11041 gtaaagtagg cttccttgat cacagcagca cttctcttag gatagtctac tttcacgatt
    11101 tggatagggg taggcttttt atggataacc gccaacccgc gggaaagata atatttatta
    11161 ctttcattga ttgcttcttc aaagcgcatc ccccgattgc cgaattcagt gatctgattt
    11221 cttttttctt gaattttttc gacgggagag gcattcttat aataaggttg gccatttgga
    11281 tagcgcaata ccatctttaa tcacactcct aggccacatt atagcaaaaa agcggataga
    11341 aagaaaagga atctgagatg gataagtcaa aagtaatcta tgtgtctggg tatcgaagtt
    11401 tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca agttgttaaa aatgttttaa
    11461 aaaaagaact ccatcagtta gcagaagaag ggctggaatg ggtacttgtc agtgggaatt
    11521 tgggcgtcga actatgggct gtgcaaatag tagcggaatt gaaagaagaa tatccggaac
    11581 tatctcttgg tattttgtat cctttttctg attttggtaa taactggaat gaagggaacc
    11641 aagaaaaaaa acaattagca gaacaattag cagattatgt cgaagctgtt agccatcaac
    11701 cctaccaatc acctagtcag ctaaagaatc acactcgttt tatacttgag catacagtgg
    11761 gctgtctgct gatctatgat gaagaattcc ctggaaaaac gaagtatttt tttgaagatg
    11821 cacagaatta ccaagcagat catccctacg aaattcggct gatcagtatg gacgatcttc
    11881 aaaattttag tgaataatcg atattgtgtt tgatttatca acttttttcc tatacaatga
    11941 aaaaggtaga aatgttatta cgtaaatgaa aactagaggt gtaaatatgg cgaatttggt
    12001 ttatagtcct aaagacattt tacaacaaga attcaaaaca aaaatgcgcg gctatgatcc
    12061 cgttgaagtt gatgaattct tagacaatat catcaaagat tatgaagctt acagcaaaga
    12121 actccttgct ttacaagaag aaaacgatcg attgagtgca aaagtagctc aattatctaa
    12181 aactcaagga gcagctcaaa cacgcgtgca acaaacagag gcaccaaaga gcgcagcagt
    12241 gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa cgtgaagtat ttggtaaaaa
    12301 gcttgatcaa caagcaagtg cagtcaaacc agcacaacct aatccaaata actacacaaa
    12361 tgcagacaca agcttagatg ataatgaaaa aacacgtcaa ttttaatttt tgattaatga
    12421 atttgcggtt ttcgggtaat cgcggtctga cttgatcagg ctgaggaaag tccatgctcg
    12481 cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac cataagctaa ggtactcttt
    12541 ggagtaacgg caggaaaaca agctaaggct tcggctatgc ttaagtatcc ttgaaagtgc
    12601 cacagtgacg aagcggctgg ggaaacccat ccggtggaac gcggtaaacc cctcgagcga
    12661 gcaacccaaa caatggtagg ggcgcttttt gtctggaaga agaacaggac aaaaaggcag
    12721 tataactgca gatagatggt taccgctttg ttttttctcc ctggaaaagc aaagtacaga
    12781 acatggctta tagaaaaccg caaacatcag ggaattaggt cgtgacaaaa gtaatgcgtt
    12841 ataagaaaca agaaaaacat ttgaaagcta tttttcaata cttcagatga atcgttctta
    12901 ttacaaaaca aacttttgta tcaccggtta ttcggaattt gagtaaaagt gtggggtagg
    12961 actgatgtcc cacctccttt ttttataaat aaggagattg tgtatggaaa aaaacaggaa
    13021 gttcaacctc gtcgcaactg cggcaagcgg gctagaagca ctggttggta aagaattaag
    13081 agatcttgga atcgaatgcc aagttgagaa cggacgtgca cgttttgaag gaacaatgga
    13141 aacgatcgct acagctaatt tatggttacg tacagcagat cgagtaaaaa tcgtggttgg
    13201 ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac gtaaaagcat tgccttggga
    13261 agattttctt ccactagatg cagcttttcc tgtagctgga aaatcgatca agtcgaaact
    13321 atatagcaca cctgattgcc aagcgatcac aaaaaaagca atcgttgaac gtctccgtac
    13381 ctattatcac cgtccagctt ctgtaccact gacggaaact ggcgcacatt ttcagttgga
    13441 agtagctttg ctgaaagacc atgtgatggt aactctggat acgacaggac caagtttatt
    13501 caaacgtggt tatcgcttag aaaaaggtgg agcaccattg aaagaaaaca tggcagcagc
    13561 acttattatg ttgacaaatt ggcgaaaaga ccgtccattt tatgatcctg tatgtggttc
    13621 aggtacgctt tgtattgaag cagccttaat aggccataat atcgcaccag gattcaatcg
    13681 tgagtttgct tgcgaatctt gggattggtt cagtcaagaa atcatggaaa atgttcgatc
    13741 agcagccgaa gaaaaagcag attatgatat tgaattagat attactggtt cagatatcaa
    13801 tggacggatg attgaaattg ccaaagccaa tgcagaagaa atcggattag gctcttcgat
    13861 tacctttaag caacaagcag tcaaagactt caaaacggat aaagaatacg gggtcatcgt
    13921 ggctaatccg ccttacggag aacgtctagg agaagaagaa actgttcgga aattatataa
    13981 agaaatgggc gatgtattcc gtccattaaa aacatggagt aaatatatct tgactagcga
    14041 cttagcgttt gaggaatttt acggacaaaa agcaaccaaa aaaagaaaat tgtacaatgg
    14101 tgcattgcgt acggatctat tccagtattg gggaactcgt ccgcctagaa aaaaagagaa
    14161 agacgaataa tcttactaat tgattcattc aattggatgt ttggaggaat agggaatgaa
    14221 agaaaaagag tttttacaag aagtaaaaga aatcaatctt ctacaacaag ctttaggaat
    14281 cctcgattgg gatacacaaa caggtatgcc tgaaaaagct agtgcatacc gaagtgaagt
    14341 agacagctat ctttacagca tttatttttc aaaaaaaatc ggtccaaaaa tcaaagaagc
    14401 cattcgctat ttttctgaac atccggaaga attgtcggaa gttggaactg ccgtttttgc
    14461 caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg gaagatctta tgagaaaata
    14521 tacagctgcc gtttcttcag cacacggaca atggcaaaaa gctagagaaa cacaacaatt
    14581 ttctgatttt caacacgcat tgaatcaaaa tattgaatta acaaaacagt tgatacctta
    14641 ctggagaaaa gatgaaaaga cgaattatga tgttttgctg aatcaatacg aacccgggat
    14701 gactgtcgaa atccttgata aggtatttga tcagcttaaa gaaggaattc tttcgatccg
    14761 ccgtacgctc caagaaaaag ggactgaacc tgaaacagac tttttaaatc ggagaatgac
    14821 gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa caacttggtt atgattttag
    14881 tcgtggtcgt ttagatgaca ctgtccaccc atttatgact ggaattaatc cgaatgatgc
    14941 acgaatcacg acacgatgga acgaaacaga tttcaaaatg gctgtttttg gagtgatcca
    15001 cgaggctggt catggtatgt atgaacaaga catcgatgat aaatatgcgt atacaccggt
    15061 ttatgaaggt acatcaatgg gtatccatga atcacaatcg ttgtttaacg agatcattat
    15121 tggaagtagt cgcagcttct ggaaaaagca atatcctttc tttcaagaat gtgcagaagg
    15181 aacattcgat gatattcctt ttgacaaatt ttatcgttca ttgaaccata caaaatcaag
    15241 tttggttcgt atcgaggcag acagtttgac ttaccctctc catatcatca ttcgttatga
    15301 aatcgaaaaa atgattttca atgaaggggt agatgttgct gaactacctg aaatatggaa
    15361 tcaaaaatat gaagagtatc tgggaatacg accaacaaat gatgtggaag gcatcctaca
    15421 agacgttcat tggtcaggtg caagctttgg ttattttccc tcttatgcat tagggtacat
    15481 gtatgcggca caattacttc attctttgca gcaagaaata gatgtggaaa atgttctagc
    15541 gtcagacgat tattctccaa tcaaaaactg gatgtcagag catatccatc aatatggtgc
    15601 atctagaaaa ccaaatcaat taatcatgga tgcaacacat gaaccgctta atccgcaata
    15661 cctgatcgac tatatgaaag acctgtattt taatgtatat caggtaaaat aaaatacaat
    15721 ttacaaagat agctgaaatt catctaatct atagatgttt tcagctattt acttaagaaa
    15781 gaagtggaac gaatggataa attacaagtg atcgctgatt atagtatgga ccaattagcg
    15841 catgatcaaa caggacatgg gagcgatcat accaaaagag ttgtcaagtt agctgaaaaa
    15901 attttggaga cggaaccgca ggcagaccgc tttgtgacgc ttgcagcagc ttacctacat
    15961 gatacgattg atgataaagt cgttaaaaat gagaacgagg caaagcaaca gcttcgtgta
    16021 ttccttcgct ctttgccaat agtagaggaa cagatctcga tgattttttc cattatcgaa
    16081 aatatgtcct tttctaaaaa tctctctgaa gcggccgaac tatctctgga aggtaagatc
    16141 gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg gcattttgcg tactgcttat
    16201 ttcggcggtg gacatgggca cccgattttt gatcctgaac tgtatccgca aacattcaag
    16261 gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc atttttatga aaaactgttc
    16321 ttgctgtctg ataaaatgaa tactgattac ggatatgaag aagccaaacg aagagaaacc
    16381 ttcatgcgtg aattcttaga agaatttttt ctggaatggt ctattgacga tgaaaatttt
    16441 actccagaca attggatcaa aaataacaaa aaatgatgga ggttgtgaca gaagtggtca
    16501 gctccaagat atttcggatc attttccgta aaagttactt ctggaacact gtttaccaag
    16561 aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa aatagcttct
    16621 cgtgtttgat gaatattgta acttgtgatt cggaattggc aggagaacac cgtttattcg
    16681 gaagaatagg actgtgacaa gacttttgtc acagtccttt tttcgaattc aacaagtaaa
    16741 acttttaaga aatattcaga gagtaaaatt aaaaattgct gataaacgaa gatggaaatt
    16801 ttatgtattt ttattggtct atgcaataaa taaattaaat attcagaata caattgattt
    16861 taacgaaagg gcttctatca atggttgtgt tgtatttatc tgcccaggta gagtaaaata
    16921 gatcattatc taaaaggtaa ttgttcatac tatataagtc atttgaaagt aaaaagatac
    16981 tgtaaataaa aaatgtggtt aaataattct cataatcagt gtaagcgtta tcaaaaatat
    17041 agcatacttg ctttgtagat gaatgacacg atatatgttt ggtatgttct gcaagacaga
    17101 tgaatatgga ggtggagaca atgtcttcca aagaactaac aatgtattta gagaaagaat
    17161 tggatcaact gaaagaaaag gggttataca atacgatcga catactagaa agtgaaaatg
    17221 gtgcttgtat catcgtagac ggaaaaaaga tgatcaatct agcatccaac aattatcttg
    17281 gatttgccaa tcgcgaagaa ttgaaaaaag catgtattga agcaaccgaa acttatggtg
    17341 taggagcagg agcagtccga acgatcaatg gttcgctgaa aatccatcag caattagaag
    17401 aaaaaatcgc agaattcaaa gggacagaag cagcaatcgc ctttcaatct ggattcaact
    17461 gcaatatggg cgctatctct gcagtaatga caaaagaaga tgctatctta tcggatgaat
    17521 taaaccatgc atctattatt gacggttgcc gcttatccgg agcaaaaatc atccgaatta
    17581 aacatcaaga tatgaaagac cttgagaaaa aagcaaagga agcgatagaa agtaagaaat
    17641 ataaaaaaat catgtatatc acagatggtg ttttctccat ggatggagac attgcacgat
    17701 tgccggaaat tattccgatt gttgaaaaat atggactgat cacctacgtc gatgatgcgc
    17761 acggctcagg cgtaactgga aaaggtgcag gaaccgtcaa gcattttgga ttaagcgaca
    17821 aaatcgatat gcaaatgggc acgttatcga aagctatagg tgttgttggg ggttatgtag
    17881 ctggatcgaa aacactaatc gactggttga aagctagaag tcggccattc ctattttcta
    17941 cttcgctaac tccaggtgca gctgcagctg cattggcttc gattactttg atgcaagaac
    18001 atccggaact tgtcgaaaaa gtttgggaaa atgctaatta ttttaaagaa gaattgaaaa
    18061 aagtaggcta taacatcggt atgtctgaaa cacctattac tccagtcatt ttgggagacg
    18121 aaaaagtgac tcagactttc tctaagaagc tgatcgaaca tgggatttat gctaaaccaa
    18181 tcgtttatcc aactgtgcct cttgggacag gcaggatcag aaatatgccg actgcggaac
    18241 atacgaggga aatgcttgat gaagcagtag cagtttatca aaaaatagga aaagaaatgg
    18301 aaattattta actaaattta gttttttcgt ctatttaaaa ttagagaaca tgttcacgca
    18361 tatattgtta atcatctgaa gatagtagta aaaaagatca gggaggatca tcatgaaaaa
    18421 agtattggtg acaggatgtt taggacaaat tggttcagag ttgatcacac ggctacgtca
    18481 agaactaggc agtgaaaatg tcattgcgac agatatacgc atgcctgaag tcattgagaa
    18541 aaacgatatc tttgagatcc tagatgtaat ggattacgaa aaaatgcgca gcatagcaga
    18601 acaatatcaa gtggacacat tgattcattt ggctgctctt ttgtcagctg ttgcagaagc
    18661 aaaaccacaa ttagcctgga atttgaacgt gactgggctg gtcaatgcat tggaagtcgc
    18721 tagagaattc cagctgaaat tttttacgcc aagttcaatc ggcgcgtttg gtccaaatac
    18781 tccaaaagat cataccccac aagatactct tcagcgtcct acaaccatgt atggggtaac
    18841 gaaggtagct ggagaactac tatgtgatta ttattatgtg aaatatggcg tagatactcg
    18901 tggtgtccgt ttccctggat tgatttctta tgcaaccttg ccaggaggcg gtacgacaga
    18961 ctacgcagtc gatatttatt attcagcaat caaagaagga aagtacacta gtttcattga
    19021 tcaaggtaca tcaatggata tgatgtatat gccagatgcg atcgatgcca ttataaaact
    19081 catgaatgca gatcctgatc gattggttca tcgaaatgcc ttcaatatat cggcgatgtc
    19141 ttttgacccg gaagagatca aagcatctat tcaaaaaatc ataccagatt ttgaaatgga
    19201 ttatgatgtg gatcctgtac gtcagtcaat agcagaatct tggccaaatt cactagatgt
    19261 ttcttgtgcg caagaggaat ggggcttttc gccgaaatac gacttagatg tgatgacaaa
    19321 ggatatgttg gaaaaactga aagaaaaatt ggtagagacg gtataaaaca agaaccacat
    19381 ccaaaaagtt ttttatactt ttgggattgg ctatggtatg aaaggggaca agggatggaa
    19441 gaatatcaag ccacgccagt aaaagaagta aaaatagaaa atgaattaaa acgtacgatg
    19501 ggatttttta ctgctttatc gactgttatg ggcacagtga tcggtgcagg ggtatttttt
    19561 aaagccgcaa gcgtagcaga agttacagga tccgccagtt tgcatatgtt ttcttggttt
    19621 ctcggtggta tgatttctgt ctgtgcagga ctaacaggag ctgaactggc agctgctatt
    19681 ccagaaacag gagggatgat taaatatatc gaacggattt acggaaatac agcagctttt
    19741 ttattgggct gggcgcaagt cgtgatttat tttccagcaa atgtagcagc cttgtcgatc
    19801 attttcggta cacagttcgt caatctattt ggtctttccc agtcaatgat tgttcccgta
    19861 gctgttacag ctgctgtatc gatcctgttg atcaattttt taggttctaa agctggaggg
    19921 gcttttcagt cgattacgct tgtctgcaag cttattcctt tgtttgtgat cgtcattttc
    19981 ggtttgttcc gacaagaagg agtagacttc caactgtttc cgatccaagc aggagaaaat
    20041 ctttcatttt tctcagcctt gggagcgggc ttgctagcaa cgatgttcgc atacgacggt
    20101 tggatacacg taggaaatat ttcgggagaa ttgaaaaagc cggcgaaaga tttgccaaaa
    20161 gcaatttctt taggaatcat cggaatcatg atcgtttatt tattagtaaa tgcggtgttc
    20221 ttaagaaccg catctatcga cggagtcgca ggaaacagca atgcggcaag tgacgttgca
    20281 aaaatgattt ttggcggctt cggtggaaga ctggtcacag ttggtatctt gatttctgtt
    20341 tatggaacca tcaatggcta tacccttact ggaatgcgtc tcccttatgt tatggcaaaa
    20401 gaaaacaacc tgccttttag caagctcttt gctaaacttc atgataaaac taaagttcct
    20461 gtagccgcag gtatcttaga gctagtcatc gctattggca tgatgatgat cggtggtttt
    20521 gatacactaa cggatatgct gatctttgtc atttggattt tttatacgat ggtctttgtt
    20581 ggtgtcattc ttcttcgtaa aaaagaacct gatttgtttc gaccatacaa agttccaatg
    20641 tatccgttta ttccactagt agcaatcatt ggagggactt ttatcgtatc aagcacgctt
    20701 atcactcaga cctttttagc ttcgatgggg atagtattga cacttgctgg tatcccgatt
    20761 tatctctatt taaaacgtag atagctaaaa cattcgaatt ttattagaac tgcacttctc
    20821 tgccaaacaa aatattttat ttaaatgagg agtacgtgga agaaatgtca aaagtactcg
    20881 ttaattttga tcacgtaaat gtcgttttgt ctacgatgaa atagcaaagt aaaagacaga
    20941 aatcgggctc aaccgatttc tgtcttttac tttgatttgc gtaactgaac tattcggcgt
    21001 acgccgcgat aaatatttaa taaaaccaag caaccagaaa tgaaaatggg aagccaaatc
    21061 agatagacag ggtagccaag cactttagac aaagcatgtg gagtcatttt atccatttca
    21121 agaagtgctt gctgccaagt ctgaagattg aagactagca tgaaacccga aataatcagg
    21181 agaataaatc ctaaataatg aattgtagtt ctttttctta cataagcgat cgtagcataa
    21241 aggaacagta aaaaaatagg tacgccaaaa acaaagtaca tattcagcat ctcgacaccc
    21301 cctttgtgtt tatcttactt tactttgaaa gaaatgagaa ataatagcct cgttacttgc
    21361 ttgaaacagc aaaggactgc taagataaga gagatacaaa gaatcaagga ggagtcatat
    21421 ggcaagttat atcgaaccga ttcgttttgc gctgattgtt tttccatttt tagccttagc
    21481 gatctcatct gtttttttta tttatgaata tcgaaagtat ggaacatttc tagtaacaag
    21541 ggcagttatt ttgtattctt tcgtttttta tttattgtgt gcgtattttt tggtgatctt
    21601 accacttccg gcaaaggaga cggttgccca aatgacaggg ccaaagatgg agctgcattt
    21661 atttgcttca tggcaacatt tcagagacaa cacggtattg gtcttgtctg aacctagtac
    21721 ctacctgccg gcaatgaagc agagtgtatt tttggaacca gcttttaatg ttttattact
    21781 cataccattt ggcatttatt taagatatta tttccggctt tcttttatga aaacgatgat
    21841 tgctagtttc tgcctttctc tattttttga attgacgcag ctaagtggac tttattttat
    21901 ctacccaaga ccataccgct tatttgatgt gaatgattta gtgacaaata cattgggtgg
    21961 aatcattggt tttgtcgtta cgccgttatt tacatttatg cttcctacaa gaagacgaat
    22021 ggatgaactt tcttatgaaa aaggaaagac tgtctcattg actcgacgct tggtcgctta
    22081 tttgatcgac tggacatttt tatctcttat taatttggta gcggcgattt tctttaaaat
    22141 tgcgttcgat tcaggggcag ttacttctca tatctggtgg cttttattag aagtaggggt
    22201 atactttatg attgtaccgt ttataaccaa tggatggaca cttggcaaaa aaattgtgcg
    22261 tatccgtttg gtggaagaag accgcgaacg aatttcttta aaagcattga tgatccgcta
    22321 cgggtatctg tactttcttt tttatggttt aagctggttg gggcagttgc ttcagcaaat
    22381 catgaattcg acaacgaata cggtgttgca gatgagtgca gtattcattt ctctcggtgt
    22441 cttgttacta caaggtattt ttattttaaa tatcttgcta tcatttttga gaaaaaaacg
    22501 gattctgttt tatgaaaaag ctagtcacac atatactgtt agcacgattg gccaaacaaa
    22561 ggatgatgat tctcgaaatt agctgagcaa aaacgctttg tctgtgttat aatgccaaag
    22621 tcgtgataat ttaaagagaa gaggtgcacg aatgaataat tttgatatat tccgcttcaa
    22681 accgtttatc aatgaagcgt taaccgcaaa gggcttcaaa cagcccacag aggtccagga
    22741 acgtttgatt cctttaattg caaaaggaaa aaatatcatt gggcaatctc aaactggtac
    22801 aggaaaaaca catacgtttt tgttgccttt gtttgaaaaa atcaatgcag aaaaagagga
    22861 agtacaagtt gttattacag ctccaagtag ggaactagct gcacagatct atgaagcagc
    22921 tacacaaatc gctaaatttt ctacaccgga gatccgtgtc tcgaattttg tcggcggtac
    22981 agacaaacag cgtcaattaa atcgtctgca tcaccaacag ccacaggttg tagtaggcac
    23041 gcctggtaga atattagaca tgatgaatga acaagcgctg aagattcaca ccgcatttgc
    23101 atttgttgtc gatgaagcgg atatgacatt ggatatgggt tttttaaatg aagtagacca
    23161 aattgcaagt cgattaccag aaaagctgca gtttttagtt ttttctgcca ctattccaga
    23221 aaaacttcgt ccattcttac gaaaatacat ggaaaatcca atcattgaag aaatcaaacc
    23281 aaaagcagtt atttctgaag acattgaaaa ttggctgatt tcaacaaaag gtaaagatga
    23341 gaatcaaatg atttatcaac tgctgacaat tggtcatcct tatttagcaa tcgtatttgc
    23401 caatacaaaa cagcatgtgg atgaaattgc cgattacttg aaaaaccaag gcttgaaagt
    23461 agcaaagatt catggagaca tcaccccaag agagagaaaa cgagtgatgc gacaagtaca
    23521 gaatctggag tatcaatatg ttgtagcgac tgatttagct gctagaggga tcgatatcga
    23581 aggtgtctct catgttatta atgcagaaat tccagaagat ctagacttct ttatccatcg
    23641 tgtcggacgt actggacgta atggcttaaa aggaacggcc attacgttat attcgccaaa
    23701 tgatgagcaa gccattgatg cgatagaaaa attgggcgtc tcatttgaac caaaagaaat
    23761 caaaaacggg gaaatcgtca aaacatacga tcgtaataga cgtgccaaac gagaaaaatc
    23821 aaaagatgta ttagatccca cgctgattgg tcttgtgaag aagaaaaaga aaaagatcaa
    23881 gcctggttat aagaaaaaaa tcgattgggc aattgcagaa aaaaataaaa aagaacgcaa
    23941 aatcgaacgc agacagcaaa gccgtactgc tcgtaaaaat aagaaaaatt cacataaata
    24001 aaaaacaaga ggttgtgaca aaaggcgtgt cacaatctct tgtttttaag ctagactttt
    24061 tttcattccg atacaaagta cttcatcttc aataaaaggt tctgtgcaag tatgataccc
    24121 ttgtttttca taaaaatcta cagccgtcat ttcagctact acataactag tctgacaacc
    24181 ttctgaaacc gcccgttttt cagcagcttc aagcaactga ccgcctaacc cttttcctcg
    24241 gtattctttt gctacgcaaa atcgttccgg cttgaagcaa gtaggtgttt tcttttcata
    24301 acgcaaagta gctactggtc ttttttgatc aagaaataga taataggttt gttgcgggat
    24361 atcttttccg tcgaattcta gttctgcagg aatcccttgt tctaatacaa aaacttgata
    24421 tctcaaaaag cagctagctg cgcgtatcca ttcatccgct ccaactattt ttttcatcat
    24481 attcctcctt gtcatgaata gcagagatgt attgattcct ttattcttat cgtaccaaaa
    24541 atcgtggagt atgaccaaaa ggaaatgaaa ataaaaattg agatattgac agtgatcaat
    24601 agaatgcgta tactaattaa gtacgaaaag gatatgattt gttttttaag actcacagaa
    24661 aattctttct tggctgaaaa aagaatagtc actaatcaaa ttgctccctt tttctatttt
    24721 ggtagaaata ccgacgcagc tcagcgttat cgagtttaag aggtaatgat tacacatcat
    24781 tgcaatcaag gtggtaccgc gagttttcgt ccttggctgt gatggtgttt ttttgtattt
    24841 aatttataga aagcaggtaa tgacatgaaa caattaagta gtgcacaagt tcgacaaatg
    24901 tttcttgatt tcttccaatc taaaggacat acgattgaac ctagtgcgtc tttgatcccc
    24961 gtgaacgatc caaccttatt gtggatcaac tctggtgttg caacattgaa aaaatatttt
    25021 gatggttcag ttgtgccaga aaatccaaga atcacaaatg cccaaaaatc tattcgaaca
    25081 aatgatatcg aaaatgtcgg taaaacagca cggcaccata cgatgtttga gatgctgggt
    25141 aatttttcga ttggtgacta tttcaaaaat gaagccatcc actgggcatg ggaatttttg
    25201 actgatccaa aatggatggc attcgatcca gaaaaacttt atgtaacagt ttatccaaaa
    25261 gatacagaag caaaacgcat ctggcatgaa gaagttggcc tgccactaga tcacattatt
    25321 gatgtagaag acaacttctg ggatatcggt gcaggcccat gtggtccgga tacggaaatt
    25381 ttctatgacc gtggagaagc ttataacgat ttagcagaag acgatcctga aaactatcca
    25441 ggaggagaaa atgaacgtta tttagaaatc tggaacttag tattttcaga attcaaccat
    25501 aagcctgata acacatttga accacttcct cataaaaaca ttgatacggg gatgggattg
    25561 gaacgcatgg tctcaatcgt tcaagatgcg tcaacgaact ttgagacaga cttgtttatg
    25621 cctattatcc acgccgtaga aaagctaagc aaacaagtaa aatatggtga aaacccagta
    25681 acagatattt cttacaaagt aatcgcagat catattcgcg ccctttcatt tgctattggc
    25741 gacggtgcat tgccatcgaa tgaagggaga gggtatgtct tacgccgttt gcttcgtcgt
    25801 gctgtcatgc atgggaaaaa attaggtatt gaacaagcat tcttatatcg tttagtccct
    25861 gttgttgggg aaatcatggt aagttattat cctgaagtac ttcaacaaaa agaatttatt
    25921 gaaaaagtta tccgtacaga agaagaacgt ttcaatgaaa caatcaatga aggtttggaa
    25981 atcttaaatc aagtgatcca agaagtaaaa aacgaaaacg ggacgactct agcaggaaaa
    26041 gacatcttca aactatacga cacatatggc ttcccggtag aattaacgga agaggtggca
    26101 gaagatgaag gactgaaagt tgatcatgct ggttttgaaa aagaaatgga tgcacaacgc
    26161 gaacgtgctc gctctgcccg cagtactgaa aaatcaatgg gggtacaatc tgctttattg
    26221 acagatatca aagtagaaag taaattcatt ggatacacag aacaagaatc agatagtcaa
    26281 ttattggtca tcgttaaagg tgagtctctt ttgaatgaag tcgcagaagg tgaagctcaa
    26341 atgatctttg atcaaacacc tttctatgca gaaatgggtg ggcaagtagc ggatcaagga
    26401 tggatcatga atgaagtagg cgaaatcgta gcagttgtta cagatgttca aaaagcgcct
    26461 aatggtcaat ttatgcacac cgtagaagta aaagcaccaa tgagtgaagg acaaacctat
    26521 catctattga tcgatcaatc gatgcgcaat cggatcatca aaaaccatac agcaacccat
    26581 ctactgcaca aggcattgaa agaagtactt ggcgagcatg caaatcaagc tggttcttta
    26641 gtcgctcctg gacatttacg attcgacttt acccactttg gtcaaatcac ttcagatgaa
    26701 ttagctgaaa tggaaagaat cgtcaacgaa aaaatctggg aagctatccc agttgaaaca
    26761 attgaaacag atatcgatac agccaaaaac atgggtgcaa tggctttgtt tggtgaaaaa
    26821 tacggacatg acgttcgagt agtaaatatt ggcggctggt caatcgaact gtgcggaggc
    26881 acacatgtag caaatacaga aaacatcgga atcttcaaaa tcgtttctga atcaggaatt
    26941 ggtgcaggcg tgcgacgaat cgaggctgta acaagtaaag aagcgtacga actacttgaa
    27001 gaagaagaaa aacaactaaa acaagtagca acaattgtta aatcacctca attgaaagaa
    27061 gttgtttcaa aagcggaaca attgcaacag caagtaagag agctacaaaa ggaaaatgaa
    27121 caattagcta gcaagttagc gaatcaacaa gctggtgatg tattcaaaga tccagaaacg
    27181 atcaatggaa aaacaatcat tgcagcacaa gtaaaagtga aagatatgaa tcagctgcgt
    27241 caattagctg accaatggaa acaaaaaaat atttcagata ttcttgtttt agcaaacgaa
    27301 caagaaggaa aagtcagtct gctagcggca atgacgaaag aatcaaatga agctggacta
    27361 aaagctggcg acttgattaa agcaatcgca ccgaaagtag gcggtggcgg tggtggacgt
    27421 ccagacatgg cacaagctgg cgggaaaaat cctgctggta ttccagatgc acttaaagct
    27481 gcgaaagaat gggttgcgca acaatagggc gtttacgttt gacagaaaaa caaaagttca
    27541 aacattgaac gaaacatgat tatgggggcg aagacaaaca agtcttgccc tcatactttt
    27601 ttcaattgga tggcgcatct aatcaaatgg tcggattcgg atggtcttta gttgacgaaa
    27661 gtgacagaaa tttttacgat tagtataaat aaaaaggggt gaaaaaatga atagtagaaa
    27721 taagaccttt cgactagtct tacgtgcgat cttgttagca atcatcatcg tacaagcgat
    27781 ggttccttgg ctggggttta ttccattagg attcattagt ttaacaatta tccatatcac
    27841 agtaattatt gctgcagtag tattaggccc aaaagatggc atggtgattg gcttgttttg
    27901 gggaatcgct acaattgttc gtgcctatgc catgccaaca acgccatttg atacattagt
    27961 gttcacgaat ccgattatct cggtagttcc cagagtactt gttggcttag tagcaggctt
    28021 ggttttccac tggatttatc aaaaaaataa atcaataacg atcagctcag cctttgcagg
    28081 ggtactgggt tcattggtca atacagtctt agtgttagga ttcatgggat tattttatac
    28141 aggtgcaacg gcagatgcct atggtgtaga ctctgctctt ttatttaaaa ccttagcagg
    28201 ggttgcagca atcaatggga taccagaagc aataggtgcg ggaatcataa caccattact
    28261 agcaaaagcg ttatttgcag caacaccgtt gaaaccagaa taatgaccat cttttaatag
    28321 taataggacg cacgatagat agaaaaagga agagaggaga ggaaaacaat cgtcctctcc
    28381 atctttttac ctgagcaaca agcaaactag aaggggtaaa aaacttggct aagaaacaag
    28441 aagaaaaaaa gaaacaaata ccgcaacagc ggacaaaaaa aggagtacta gcgggtatct
    28501 taggattggt tacgaatctg atcttgtttg tctctaagct aatgatcggg cttttttcag
    28561 gaagtgtctc cattatggct gatgcagtga acagtctatc agatactgct tcatccatat
    28621 tgactttgat cggatttaaa attgctgcaa agccagcaga tcaggaacat ccttatggac
    28681 acgaacgctt cgaatatatc agtgggttat ttgtttcgat catcattact tatgtaggat
    28741 ttcaattttt agagtcttct gctgaaaaaa tcatccatcc agaaaatgtc tctttaacac
    28801 ccatcgtctt ttttgtccta atcatttcca tctttttgaa atttgcacaa ggcaaaatgt
    28861 atcaggttat tgcgcaatcc attgattctg aaacattgag agctacagca acagatagtt
    28921 ataatgatgt ctttactaca ctggctgtat tgctttcagc agcaatcgaa tgggtaactg
    28981 gatggcgtat tgatggatat ataggttttg ctttagcatt atatatcctt tatagtggat
    29041 tgacgatgct tcgaaatttt gtatacgaac tattaggaag tcgcccaacg ccagcagaga
    29101 ttgaagaaat ggaagagcat ttaaatgctt gcgatgatat acttggctat catgatttat
    29161 tggtgcataa ctatggaccc aataaacgct ttgcatcagt ccacatcgaa gtagatgaat
    29221 caatgaattt gaatgtggct cataagatta ttgatctgat agaaaaagac tttaaaaagt
    29281 ctttggatgt ggaacttgtc tgtcacttgg atccaatgcc tgttgataac gaaaaatact
    29341 ataatatctt gcggcagtta aagcaaattc ttcttgaaac aggtacaggg cttaatattc
    29401 atgattttag agtaattcat caaggaaaaa cgctgcaatt tgatgtagtg gtccctgaga
    29461 aattccagtt tgttgataca gaactagaaa gaatcttacg tagtcaaata gaagaaaaaa
    29521 tcggagatta ccaattggaa attacgtttg atcacaatta tcttttatat taaatgagaa
    29581 actataaaaa gactccttaa agtttttaat ttaaagagtc tttttttctt ttaattgttt
    29641 atggagagcc tgacgtgcaa gattatctgc tcctttattt ttgttttcgg gcaaccattg
    29701 caggatcaat aaagaaaatt cggatagaag ttcttggatt ctttgaaggt agggaaggaa
    29761 gagctcgttc ttcgttgcgt ttttgtcgac cgtagaaact agtgttttgc tgtctgaaaa
    29821 acagaaaatc gtttcattct gtaagtcgtt tttctttaga aattctaaag tttttaaaaa
    29881 gacttcgaac tctgcttgat ggtttgtccc tatagaaagc gggatagata gctgttcttg
    29941 tttaccttga tagacaatca agattccacc tccactagga ccagggttgc cttttgtaga
    30001 ggcatcaatg tatactttta acataaaaag agattctcct ttttgttcaa actaatgtta
    30061 tggtaagatt atatcattac tgaatcggtg agaggttaga gaatgaaaaa aaatattcga
    30121 tggcagcctg aactggcaca tacgatcatc tattggtcat tgaccttgat cattctgttt
    30181 tttagtctga ttctttcttt agaaaacaca ggtccttatt ggaaaagcaa ttttgtttta
    30241 ggtatttttc ttgtttttct atttctaggt ttgcggcgtt cttttatttt aaaatcaaac
    30301 tctgtgatta tacgttatgc tgctttttgg aaagaccgag aaatatctgt cggacagatt
    30361 cgatctttga cgccaactaa tactggggtg caaatgatgt tgcaagatga gaaagcccca
    30421 tctgttttca tgatgcgaaa aaaagatcaa gtagtccttt ttgatcattt tgaaaaaaac
    30481 ggacccaaat cagaatcaat tccttatcct tatacgaaaa aaagaaaaag ctaattttaa
    30541 aaatcagaac ctaaaaaaac gaatttttac aggaaaagaa aagagtgaga cgtacggttc
    30601 atcccgttta gcgtctcact ctatttgttt atctattcat gcatactagt atcggtagtc
    30661 tgaacgttgc ctttcttgac atgggcagct tgtgttcctc gattcccttc gatgatctca
    30721 aattcgacca cttgattttc ttccaaagtt ttgaatccct cttcttcaat cgcagtaaaa
    30781 tgtacaaaaa tttcttctgt gtcatcataa ctgataaagc cgtatccttt tttgttgtca
    30841 aaccatttta caattcctgt ggtcaaagca ctcacgacct ttctaaagtc tgatcatagc
    30901 cttcattata aagagtattt ataagtacgt caaataaatt aaaaacctgt aattttatgt
    30961 tttttttctg aataaaaaaa ggtataattg gcatatctta acaaagaaga tattttgttg
    31021 aataaagttc gtgaaaaggg gtaatcaaat gaagaatgat ctgcaaattt cacaagaaac
    31081 agagctagaa ccgattgttg aagtggctag taaattagat cttaaggaag acgatttgga
    31141 actgtatggg aaatataagg cgaaaatcaa tttttctgga atcaatcgat caaaggataa
    31201 cgctgagggg catttgatct tagttacttc catcaatcca acaccagctg gcgaaggaaa
    31261 gtcaacgatc actgtagggt taggagacgc gctcaataaa atcaataaaa aatctgttat
    31321 tgcattgcga gaaccctcac ttggtcctgt gatgggaatc aaaggtggtg ctgcaggcgg
    31381 aggatatgca caggtattgc ctatggaaga tatcaacttg cattttactg gtgacatgca
    31441 tgctatcaca acagctaata atgcattatc tgctctttta gataatcata ttcatcaagg
    31501 aaatgaattg aacatcgatg cccgccgagt gatttggaaa cgggtagtcg atctcaatga
    31561 tcgtgaactg cgtaaagtaa ttgttggctt gggtgggcca atccaaggtg ttccaagaga
    31621 agatgggttt gatatcacag ttgccagcga aatcatggct atcttgtgtc ttgcggcaga
    31681 ccttgaagat ttaaaggctc gtcttgcaag gatcgttgtc ggctatacat ttgaccgtca
    31741 gcctgtgact gctggagact tgaaagcaga aggcgctctg gcattactgc tgaaagatgc
    31801 aatcaagccg aatcttgtac aaacaattta cggaacacca gcttttgtcc acgggggacc
    31861 gtttgccaat atcgctcatg gctgcaatag tattttagct accaagacag cattgagatt
    31921 agcagattac gttgtcacag aggcaggatt tggtgcagat cttggcggag agaaattttt
    31981 agatatcaaa gtacctaact tgaaaaaagc gccggatgct gtggtgattg tcgccactgt
    32041 ccgtgcattg aaaatgcatg gcggaatgaa aaaagatgaa cttaaaaatg agaatcttga
    32101 tgctttgaaa gtaggttttg caaatttaaa gcgccatata cgtaatatgg aacaatatca
    32161 gctacctgtg atcgtcgcaa tcaatgaatt cgtaacagat acagacaatg aactcacact
    32221 gttggagcac ttgtgtgaaa atcaggggat tcttgccaag agagcgagtg tatgggcaga
    32281 tggagcagaa ggcggcgttg atttagcaaa agctgtagta cgattgattg atagaaaaga
    32341 agctaattac aagccattat accgcctaga agaaacgata caagaaaaga cagaaacaat
    32401 cgtcaagaaa atttacggag gaaatggtgt tgttttcagc aaaaaagcgc aaaaacaaat
    32461 agaagagttt acgaagaatg gatgggacaa actgccgatt tgtatggcaa aaacacagta
    32521 ttcattttct gatgatcctt cactgctagg tgcaccggaa gatttcacca tcacgatccg
    32581 cgaaatcatc ccaaaattag gggcaggatt tttagtagcc ttgactggag atgttatgac
    32641 gatgcctggt ttgcctaaaa aacctgctgc attgaatatg gacgtgacaa atgacggaga
    32701 agtgttggga ttgttttaag cttgattata atctaccatc gaaggagctg tattttgact
    32761 acagcttctt tttttagacg atagggaatt aattttgttt acactaggat cattcttttt
    32821 ctttattgta ttttgagcta tactaaagca taataggagg gtatgatgaa aacgacattt
    32881 ataagaaaaa ccggttttta cggtatgggt agctcgattc agctaagaat caacaatgaa
    32941 aaacgcagtg ttctcagtca tgaacaaaag ataacgcttg atctggaagt gccctttacg
    33001 atgcaggtca ccttttattg gctaaaaagc cccgtttata cagtgaaaga agcaaagcct
    33061 gcttatgtga tcacaatgaa ttacttgatt cttcaaatct atccgttttt atttctagca
    33121 actgggttgg gggccgtttt cctacaaaat ttatttttca gtttttttgc agttattgtg
    33181 ttgattgcgt tctttttcta tataaaaaac cgggcatatt tgatcaagga ggaaaattat
    33241 ggcaaactct gatctttttt taatcagttt caatcgaatc gaaaaatgga tgagagacga
    33301 gatgggaaat gcacgaaata tgggatttac cgaacttgtc agacgcctag ctcaaagaaa
    33361 acaattaatg atcagaaaat acgaagatga tcttttacag cttgctcagt taaggaatgc
    33421 gatcgtccat gatcggatcg cagtcgattt tgtcattgca gagccaaatg attggacagt
    33481 caagcgaatc cagctgatcg aacaagaact gattcagcct gaaaccgtgc tgcctcgatt
    33541 tgcaaaacgt gtaacaggtt ttgaacaaga tcttccgtta cttgaactgt taaagatagt
    33601 agcagaaaaa aggtattcac aatttccgtt atacaataaa ggaaaatttg tagcattgat
    33661 tactttacgt aatatcgggt tttggctagc aaaagaaagc caaaaaggtc cggttgattt
    33721 aacaaataaa cgagcgttgg atttgatcat ccaaaacgga aagtacacaa attatcattt
    33781 tgttccagct aaaacacata tttacgaggt tgaagcgatg tttcgtgagc aagggacttt
    33841 ggaagccgta cttatcacta aagatgggaa tcccgatggt aatctgctcg ggatcgtacg
    33901 acctcgtgat atttataaac aaatagaaaa ggattgattt tttgttaatt atttattgga
    33961 tattcagcgc tttgctagta ggagtggatc agttggtcaa atggtggatc actgataatc
    34021 tagcgcttgg agagacgaaa aatcttatac caggtatttt gtcactgaat catattcgaa
    34081 acactggagc cgcatggagt atgctagaag ggaagatgtg gttctttact atagtgacac
    34141 tcattgcagt cgttgtgatt ctcacattga tgattaaaaa tcgtgaaaaa ggtaatcgct
    34201 ggttcatgat tggattgagt ttgattctag ctggtgcgat tggcaatttt atcgaccgta
    34261 ttcgtcttgg ctatgtagta gatatgttcc agacagattt tatgagcttt ccgattttta
    34321 acgtagcaga tgtgacactt gtatgtggag tgatttgtat gttaatttat attattttag
    34381 atgaaaagga tcaaagaaaa aaatgacaga acttaaaacg acgattcacc atgaatctgg
    34441 taggatcgat aaagtattga ctaatttatt caccgattac agtcgttcgc aaattcagct
    34501 gtggctgaaa aatggagcgg tttctgtcaa tggacagcca gtcaaagcaa attacaaagt
    34561 aaaacaagga gacgagatta ctgtcttgat ccccgaacca gaaacattag atgtggtccc
    34621 agaaaatatc ccgttgacga tcgtttacga agatgaagat gtagcggtga taaacaaacc
    34681 tcaaggaatg gtcgttcatc cttcagcagg tcatacgcat ggcacaatgg tcaatgcgct
    34741 gatgtaccat atcaaggatc tttctacgat caacggcgtt attcgtccag gtattgttca
    34801 ccgaatcgac aaagatactt ccggactgct aatggttgcc aaaaatgacc aggcacacga
    34861 atcattagct cagcaattaa aagataagac ctctttgcgc aaatatgttg ctttagtcca
    34921 tggagagatt cctcacgaaa aaggaaccat caatgcgcca atcggccgtt cgaaggaaaa
    34981 ccggaaaatg caggcgataa gagaagatgg gaagtcggct gtcacacatt ttactgtatt
    35041 ggaacgtttc gaaggattta ctctggttga attaaaacta gaaaccggaa gaacacacca
    35101 gatccgtgtg catatgaagt atatcggtta tccgttagct ggagatccag tatacggacc
    35161 gaagaaaact ttgaaaggca atggtcagtt tctccatgcg caaatgttag gttttacgca
    35221 cccgactaca aacgaaaaga tgatctttga agcacctctt ccagaagttt ttgaaaaaac
    35281 tttagaaaaa ttaagaaaaa acattgcatt ttaatttaga taacggtata gtaattaaca
    35341 acgaaagcat aaaacttaat agaattcctt taagtttagt cccgtgaggc taagaagggt
    35401 gggagcaagg atacgtatgg tgcgccataa gtagattgct cattgacata gtatagctat
    35461 cagtgtatca gtatacgctc agtctaaaca ttcctcctgt cctcttaggg caggagtttt
    35521 tttatgcaaa aaagtagggg gaatgaaaat gcaagcaaaa gaagtagtag atcaggtaac
    35581 catgaagcga gcattgactc gaattactta tgagattatc gagagaaacc atagtatcca
    35641 agatatcgta ttggtaggta tcaaaacacg aggaatctac attgcatcaa gaattgccga
    35701 acgcctgaag cagctagaag atatcgatat tcctgtagga gaactggata tcactttata
    35761 ccgagatgac aaaaaagaaa atccagaaga accggaactt cattcttctg acatcccagt
    35821 ttccttagaa ggaaaagaag ttattttgat tgatgatgtt ttgtatacag gacgaacgat
    35881 tcgagcagca atggacgcag taatggattt tggcagacca agaaaaattt cattagcagt
    35941 cctggtcgat cgtgggcatc gagaattgcc aatccgtgca gattatgttg ggaagaacat
    36001 tccaacatct cgggcggaag agattttagt agagatgcaa gagcttgatg gacaagatcg
    36061 catcatgatt ttgaaagagg aagattagag gagagagaaa catgtcggag aaaaaaggat
    36121 ttcataatga tgacgtggtg ttagatatcc acgatcgccc aaccccagga cattgggtag
    36181 gattaagttt acaacacttg ttcacgatgt tcggagcaac cgtgttagtc cccatcttag
    36241 taggtatcga tccaggtatt gcgctagtca gttcaggact aggtacattg gtgtacttat
    36301 ttactactaa aggaaagata cctgcgtact taggaagcag cttcgctttc attgcagcga
    36361 tgcagctttt gatgaaaaca gatggttatc cagcgattgc acaaggagca gtcacaactg
    36421 gattagtcta tttgattgtt gcgctgatca tcaaaaagat cggttcagca tggctggata
    36481 aaatcttgcc tccaatcgtc gtcggtccag tcatcatggt tatcgggttg ggacttgcgg
    36541 caaatgcagc aacaaatgct atgtataatc agggcacgta tgatttcaag tatattgctg
    36601 tagcgctgat cacattagga ttaacaattt tcttcaacat gtggctaaga ggttttctcg
    36661 gattgattcc gattttacta ggtattatta gcggatacat cgtagccttg ctgtttggaa
    36721 tcgtggatac taagccaatc gtagacgctg cctggtttgc tgttcctaac tttgagattc
    36781 cctttgtaca atatgaacca aagctatatc ttggagcaat cacaacaatg gcacccatcg
    36841 cttttgtgac gatgacagag catatcggcc atcttatggt attgaataaa ctaactaaac
    36901 gtaatttctt tgaagaaccg ggtcttcata agacgttgac aggtgatgga ttagcacaaa
    36961 tcgtggcagc ctttgtcgga ggtcctcctg taacaagtta cggtgaaaac attggtgttc
    37021 tggctatcac acgtgtccat agcgtgtttg tgatcggtgg agctgcagta tttgcagtca
    37081 ttttaggatt cgtaggaaaa ttaagcgctt tgattttaag tatccctgga ccagtcatct
    37141 caggaatcag ttttatctta tttggagtaa ttgccgcaag tggaatgaag attttaatcg
    37201 agaacaaaat cgattttgat aaaaagaaaa atctgttgat tgcttcagtt atcttagtag
    37261 taggaattgg cggactcgtc tttgaagtag gcacatttac attgtcggca atggcattag
    37321 caactgtact tggcatcatt ttaaatctta tcttaccaga aacttcacga agcgaagaaa
    37381 actaaggagg aaacattatg atcatcaaat cagaacgtat cagcttaaag catttattga
    37441 ccgttgaggc acttactgat caagaagtta tgggcttgat ccgcagaggg caggaattca
    37501 agcaaggtgc taattggact ccgcaaaagc gccagtactt tgctacaaac ctctttttcg
    37561 aaaatagtac tcgcacacat aaaagcttcg atgtagcgga gaaaaaacta gggctagaag
    37621 tgattgaatt tgaagcaagc acaagctctg tccaaaaagg ggaaacacta tacgatacag
    37681 tactgacgat gtcagcatta ggcgtagacg tagcggttat ccgtcatggt gatgagaact
    37741 attatgacga actgatacaa agtaaaacaa tccagtgttc gattatcaat ggaggagacg
    37801 gaagcggaca gcatccaacc caatgcttgc ttgatttgat gaccatttat gaagaatttg
    37861 gtcattttga aggtctgaaa gttgccatcg taggtgatat cactcactct cgcgtagcta
    37921 aatccaatat gcagatgtta aaacgtctag gcgcacaagt attcttctca ggtcctagag
    37981 aatggtacga cgatgaattc gaagtgtacg gacattatat gccattggat gaactactag
    38041 atcaagttga tgtcatgatg atgcttcggg tgcaacacga acgccatgat ggaaaagaaa
    38101 gtttctcaaa agaaggctac catcaggaac atgggttaac agttgagcgt gcgaagaagt
    38161 tgcaaaaaca tgcaatcatt atgcacccag caccagttaa ccgtgatgta gaattagctg
    38221 attcattagt ggaaggatta cagtcgcgga tcgttcagca aatgagcaat ggagtattcg
    38281 tcagaatggc aatcttagaa gcagtgctaa atggaaaagc gtaattatag gaggatgaaa
    38341 aaatgaaaac actcattaaa aacggtcaga tcaatacacg aaaaaatatg acgacaccag
    38401 cagaaatttg gatagaagat gggcgaatca aagcaatcgg tacaggattc tcagaagccg
    38461 aatttgatga agtgtttgat gcaaagggac aattgatcac acctggttta gtggacgtcc
    38521 atgttcattt gagagagcca ggatttacat acaaggagac gatcgaagct gggactcgct
    38581 cagctgctcg tggcggtttc actactgtat gcgcgatgcc gaatttaaat cctgtacctg
    38641 atacggcaga aaagctgaga caagtatatg acatcattag aaaagatgca gttgtgaagg
    38701 tattgcagta tgcgccaatc acagaaaatc tgcgaagtga gaaattagtt gatcaagaag
    38761 cactgattga agaaggtgct tttgctttca caaatgacgg tgtaggtgtc caaacagctg
    38821 ggacgatgta tctggctatg aaagaagctg caaaaaacaa taaagcccta gtagcccata
    38881 cagaagatga atctttatta tttggcggcg ttatgcacgc tggcaagaag gcagaagaac
    38941 ttggtcttcc cgggatctta agcgtaaccg agtcttctca aattgctaga gatctgttgc
    39001 tagctgaagc aactggtgtt cattaccatg tttgccatgt ttctactaaa gaaagtgtcc
    39061 gtgtaatcag agacgcaaaa aaagcaggaa tccacgtaac agcagaagtt tcaccccatc
    39121 atcttatttt gatcgacgaa gatattcccg aagatttcgg cttttggaaa atgaacccgc
    39181 cgctaagagg acgggaggac agagaagctt tgatcgaagg cttgcttgac ggtacgatcg
    39241 attgcatcgc tacagaccat gccccccatg gattagaaga aaaaagccaa agtttcatga
    39301 aatcaccatt tggtatcgta ggaagcgaaa cagcgtttca actgatctat actcattttg
    39361 tagaaactgg tcgattcacg ttggaacaag tgatcaattg gttggcagtt aaaccggctg
    39421 agatttttgg tttgaatgca ggaacgctaa cggttggtgc ccccgctgat gtagcagttt
    39481 ttgatattac acaaacttgc acgatcgata aagaagactt tttatcaaaa ggggagaata
    39541 caccttttat cggctggaaa gtaaaaggcg aaacacaaat gacttttgtc aacggaaaac
    39601 ttgtgtggca gaagggagaa taaaagtatg gaacgtttgc ttatattaga agatggaaca
    39661 gttttcgaag gaaaggcttt tggtgcagat gctagtgtct tcggagaaat cgtttttaca
    39721 acaagtatga ctggttatca agaaactatc actgatcaaa gttttaacgg tcaaatcatt
    39781 acatttacct atcctatggt gggtaattat ggggtaaata gagatgatta tgaatcgatt
    39841 tctccaacat gcaaaggagt agtagtaaaa gaacatgcac gtgtagcttc aaactggcga
    39901 aaccaaatga cgctagattc atttttgaaa caaaaaggaa ttcctggaat atcaggtatc
    39961 gatacgcgcg cccttactcg caaaatccgt gaagtcggaa cgatgaaagc tagtatagtt
    40021 gatgctggag attccttcga acatgcattt gatcagctga aagcaaccgt tctagcaacc
    40081 aatcaagtgc agcaagtctc aactaacaaa ccttatccaa gtccaggaac aggtcggaaa
    40141 gtcattgtca ttgacttcgg cttgaaacat agtattttac gagaattgag taaaagaaac
    40201 tgtaacctca cagtacttcc ctataacaca gatgccgaga cgattttagc gctatcacca
    40261 gacggagtca tgctgacgaa tggacctggt gatccaaaag atgtgccaga agcaatcgaa
    40321 atgatcaaaa aaatccaagg aaaagtaccg atttttggta tttgtctcgg tcaccagtta
    40381 tttgctttag ccaacggtgc agatacctat aaaatgaaat tcggacatcg aggattaaat
    40441 cacccggtac gagaagtagc gaccggaaga attgatttta cttctcaaaa tcatggctat
    40501 gcagtcgatg aagccacgat tgatccagaa aaactcatcg taactcatgt agaagtaaat
    40561 gacggaacag tagaaggagt caaacacaga gattatcccg ctttcagtgt gcagtttcat
    40621 ccagatgctg cccctggtcc gcatgatgca ctacatttat ttgatgaatt tatggaaatg
    40681 atggacgcag gaaaggagca gtaaacagat gccgaaacgt acagatatca agaaaattat
    40741 ggtgattgga tctggtccga tcatcattgg tcaagccgca gaatttgact acgcaggaac
    40801 acaagcctgc cttgccttaa aagaagaagg atatgaagtg gttttagtaa attctaatcc
    40861 tgcgacgatc atgacggata aagaagtggc agatcaagta tatatcgaac cgatcacact
    40921 tgaatttgta tcaaggattc ttcgaaaaga gcgtccagat gctctccttc caactttagg
    40981 cggacagaca ggcctcaata tggcaatgga gctggcagca tcaggtattt tagaagaatt
    41041 gaacatagaa cttctaggaa caaagctttc tgcgatcgat caagcagaag atcgtgatct
    41101 attcaaacag ttgatgaaag aattgaatca gccaatcccc gagtctgaaa tcgtcaatac
    41161 agtagaagaa gctgtagatt ttgcaaataa aatcggctat cctgtcatcg ttcgtccagc
    41221 ctttacttta ggcggaactg gtggaggtat gtgtgaaaat gaagaagaat tacgtatcgt
    41281 atcagaaaat ggattaagtc tttcccccgc tacccaatgt ttgattgaac gaagtattgc
    41341 aggttttaaa gaaattgaat atgaagtgat gagagattcg gctgataatg caatcgttgt
    41401 ctgcaatatg gaaaattttg acccggtggg tatccataca ggtgattcta tcgtatttgc
    41461 ccctagtcaa acattgtctg accatgaata tcaaatgctt cgtgatgctt ctctttcgat
    41521 tattcgagca cttaagatcg agggaggctg taatgtccaa ctcgctcttg atccacatag
    41581 ttttaattat tatgtgattg aagtcaatcc gcgtgtttct cgttcatctg cgttagcaag
    41641 taaggcgact ggctatccga tcgctaagct agctgctaaa atagcgcttg gattaacatt
    41701 agacgaaatg aaaaatcctg ttacaggaac gacttttgca gaatttgaac ctgcattaga
    41761 ttatgttgta gctaaaattc ctcgctggcc gtttgataag tttgaaaacg gagaacggct
    41821 tttaggcacg cagatgaaag caactggtga agtgatggct atcggtcgga atatcgaaga
    41881 gtctttattg aaagctgtgc gttctttaga aatcggtaca caccatatcg aacttcctga
    41941 gttggcagac atcacggacg atgacctaat ccaaaaagtg gttcgagcac aagatgatcg
    42001 cctgttttat gtagctgaag ctattagaag agggtacacc atagaagaat tagcggattt
    42061 gacaaaaatc gatttattct ttttagataa actgttgcat atcgtagaaa tcgaacagca
    42121 gttgaaacag cagccgttgg atgacaagat attgaaaaca gcgaaacaaa acggttttac
    42181 agacaaaaaa gtggcggagc tgtgggaaat aaacgaagaa gccgttcgcc agatgagaaa
    42241 agaaaatgat ttgattcctg tatataagat ggttgacact tgtgcagcgg aattcgaatc
    42301 aaaaacacct tatttttata gttcctatga atatgaaaat gaaagttcac gatcagaaaa
    42361 gccctcaatt ctagtactgg gatctggtcc tatccgtatc ggacaagggg ttgaatttga
    42421 ctatgcgact gtgcattctg tcaaagcaat ccagcaggca ggatacgaag caatcatcat
    42481 gaacagcaat cctgaaacag tttcgactga cttttctatt tcggataaat tatattttga
    42541 gccattgacc ttcgaagatg tgatgaatgt gatcgagcta gaacagccga taggagtaat
    42601 cgttcaattt ggtggtcaga cagcaatcaa cttagccgaa cctttgactg ctgctggtgt
    42661 aaaaatctta gggacaacga tcgaagactt ggatcgtgcg gaaaatcgtg atttattcga
    42721 gcaagcgctg aaagagttgg atattcctca acctttaggg gatactgcta caagtaaaga
    42781 agaagccgtg gttatcgctt cgagaattgg ttatccagtt cttgttcgtc caagctatgt
    42841 attaggcgga cgtgcgatgg aaatcgttga aaaccagcga gatttagaag attacatgga
    42901 acatgctgtg aaagcatctc cagaacatcc agtactagtg gaccgttact tgcttggtgg
    42961 tgaatgcgaa gtggatgcga tttgtgacgg tgaaacagta ctcatcccag ggatcatgga
    43021 gcatattgag cgtgctggtg tccattcagg ggattcaatg gctgtgtatc cacctcaaac
    43081 attatctgaa tcgatcaaga aaacgatcga agattacacg atccgcttag cattaggttt
    43141 gaattgtgtc gggatgatga atatccagtt tgtcatccat gaaaatgagg tttatgtgat
    43201 cgaagtcaat cctcgagcaa gtcggactgt tccattctta agtaaggtga caggaattcc
    43261 aatggcacaa attgcgacga aagctatttt gggagagaaa ctaaagacac ttggttatga
    43321 aaacggactt tatccagaaa caaaagaagt tcatgttaaa gcacctgtct tttcttttac
    43381 gaaattgcag aaagttgata cttacttagg gcctgaaatg aaatcgactg gagaagtaat
    43441 gggatctgac agacaattag acaaagcttt gtacaaggca ttcgaagctt ctggattaca
    43501 cttgcctgaa tatggagccg tattatttac gattgcggac gaaacaaaag aagaagcact
    43561 agaattagct aaacgtttta cagaaattgg ctatagtctg ataggaacga aacatacagc
    43621 tgcttatttc gaaaaaaatg gtctggtcgt tgaaacagta gcgaaaattt ctgaggaagc
    43681 agcagaaaaa aatgtagtcg acttaataag agatggtaaa acacaagtgg ttgtcaatac
    43741 gattgataaa gatcggggaa atgcttctaa agacggtttc atcatccgaa gagaagcagt
    43801 tgaacatggt attccattgt ttacttcttt agacacagca gatgcgatcg ttcgagtgat
    43861 ggaatcccgt gctttttcaa ctaaccctat ttaacggcga gcacatgtta agacaaaaag
    43921 acgaaaaacc gttgcaggag gggacagatg aaacaagaaa tgatggaaat cgttcagcaa
    43981 aaacagctgg caccaaagat ttttgaaatg acattgaaag gtgatctagt aaaagaaatg
    44041 cagcaaccag gacagttctt gcatattctc gtgccgcgtg cagacttgct attacgccga
    44101 ccgattagct tgaatcatat cgaccacgaa acaaatactt gccgtatcat ttatcgggtg
    44161 gaaggtgagg ggactagagt cttctctact ctttctgccg gagaccattt agatgtcatg
    44221 ggtcctttag gcaatggttt tgatttgtcc aatcttcata caggagacga agtatttata
    44281 atcggtggag gaatcggtgt ccctcctcta tatgagttat caaaacagtt agtcgaaaaa
    44341 ggaattaagc caacgcattt tttaggtttt gccacacatg aagtcgttta ttatgaaaaa
    44401 gaatttaatc gtctaggaga aacaagaatc gctactgacg atggaacata cggtatgcac
    44461 ggaaatgtcg gtaatttatt gttaggaacg aagacggaac ctacagctgt ttttgcatgt
    44521 ggttcaaacg ggttattgaa gacagtggaa cagctgtttt cttcgcatca aaatgtccag
    44581 ttatcattag aatcccgcat ggcatgtggg ataggggctt gctatgcttg cgtttgtcat
    44641 acgccggaag atgaaacagg aacaaaaagt gtgaaagttt gtgacgaagg accgattttt
    44701 aaaataggag aggtaattat atgaatgaga atcctttagc ggtcaaactt cctggcctag
    44761 aattaaaaaa tcctatcatg ccggcaagcg gctgtttcgg ttttggcaaa gaatacggaa
    44821 aatactatga tttgaatcag cttggatcga tcatggtcaa ggcaacgact ccaaatgccc
    44881 gctttggcaa tcccacgcca agagtggcgg aaacaccatc tggcatgttg aatgcaattg
    44941 gtttgcaaaa tcctggtcta gacgttgtta tgcataaact tcttccagaa ttagaggagt
    45001 acaatgagtt acccatcatc gcaaatgttg ctggtgcttg tgaagaagac tatgtagaag
    45061 tttgtagcaa aattggcgaa gcaccaaatg tgaaagcaat cgagctgaac atttcttgcc
    45121 caaatgtcaa acatggcgga attgcttttg gaacagattc tgatgttgct tttcaattaa
    45181 cacaagcagt aaagaaagtt tcttctgttc ctgtctatgt caaattgtcc ccaaatgtaa
    45241 cagacatcgt tccaatcgct caggcaatcg aagctggagg ggcagatggg tttacgatga
    45301 tcaatactct tcttggtatg cggattgacc ttaagacaag aaaaccgatt ttagccaatc
    45361 aaacaggtgg actctccgga ccagcaatca agcctgtcgc tattcgtttg attcaccaag
    45421 tagcaagtat atccagcctt ccaatcattg ggatgggcgg cgtgcagaca gtggatgatg
    45481 ttcttgagat gtttatggct ggagcaagtg ctgtagctat agggaccgcg aatttcaccg
    45541 atccgtatat ctgtcctaaa ctaattaaag agctccctgt gcgaatgagc gaactaggca
    45601 tcgaatcgct ggaaagatta atcaaagaag tgagagagga acgtgaacga tgaatcaact
    45661 agaccaacga ccaattattg cattagattt ttcaactagg caagaggtag aagactttct
    45721 tcgatttttc ccgaaagaag aaaaattgtt tgtgaaaatc ggaatggaat tgttctacca
    45781 agaaggacct gaaatcgtac gttatctaaa agaggctggt cataatgtat ttttagatct
    45841 gaaacttcac gatattccga atacggtcga aaaagcaatg cggggattgg caaagcttgg
    45901 tgttgatctc acctgtgttc atgccgctgg cggtattcgt atgatggagg cggcaatgcg
    45961 aggattagaa gaggggactc cagaaggtgg aaaacgtccg ctacttctag ctatcactca
    46021 gcttacttct acaagtgaag aagaaatgca tgccgatcaa ctaatcgaag tttctttaga
    46081 aaaaagtgtg atccattatg ctagctgtgc caaaaaagca ggattagatg gtgtggtttc
    46141 ttctgcttgg gaagtagaag cgatcaaaga aatggcagga gacgaatttg tttgcttaac
    46201 tccgggtata agaccagaag gaacagtcgc aggagatcag acaagagtag tcacaccttc
    46261 tcaggcaaaa aagatcggct caacttttat cgttataggt cgaccaatca ctcagtcaac
    46321 taatccttat aaagcatatc aaacgattca aacagaatgg agctaaccta aaatgaatgt
    46381 tgaacaaagt atcgcaaaag acttattaga aattgaagca gtatttttaa atccaagtaa
    46441 tccttttaca tgggcaagtg gaatcaaaag cccaatttac tgtgataacc gaatcacgat
    46501 gagctatcca aaagtccgaa aagagatcgc aaaaggatta gcaagcaaaa tcaaagaagc
    46561 ttaccctgaa gtacaagtga ttgctggaac tgcaacagca ggaatccctc atgccgcatg
    46621 ggtagcggaa atactggatt tgccaatggt ctatattcga agtaaggcga aagatcatgg
    46681 gaaaggtaac caaatcgaag gacgtatatt tgaaggtcag aagatggttg tgatcgagga
    46741 tttgatttct acaggcggaa gtgtcttgga agcagctgaa gcagcaaaaa gagaaggtgc
    46801 ggatatcctt ggtgttgcgg ctatctttac gtatgagttg ccaaaaggaa aagcaaattt
    46861 cgaaaaagcc gaaatccctc ttatgacgtt gaccaattat tctgtattga ttgaagcagc
    46921 tttagaagat cgttatatcg atgagcaaga attaactctg ctaaaagaat ggaaaaaaga
    46981 tcctgaaaat tggcagattg aagcgcgctt gatgaagata gagaaatccg atttgtgtct
    47041 ccagaatcat caattggaat agcaaaaatc caaaaactga ttgcgagaag catcttttca
    47101 tcaggtatga aaagaaactt ctatcgtgat cagtttttga tttttttaaa attcattttt
    47161 ctaagactgg ttagcagaaa gaagagaata atctcttctc ttttttctat tatatataga
    47221 ttttctctat tttttggtaa gggagctgtt tcaaaaatgg taaagagcgg aaatgttgcg
    47281 aatatcagtt atttaatgat atgatattcg gaaaaagaaa tctcaaaggg aggtaacttt
    47341 tatggggttg aatgaattta ttttaggatt gaacaattta gagacgatca caagggcacc
    47401 tggatttttt aaatatacag agcatacagt tgcagcacat tcgtatcgtg tagcatctat
    47461 tgcgcaagtt ctaggagaca ttgaagaagc gaatggacaa ccgatcgatt ggaaactgct
    47521 ctatgaaaaa tcattgaatc atgactacac agagcgtttt attggtgata tcaaaacgcc
    47581 ggtgaaatac gcaagcaaag agttaagagg gatgctgcaa acagttgaag aaaagatgac
    47641 ggacgaattc atcaaccaag agattccagc tgagttccaa gaagtttatc gccgtcggtt
    47701 gttcgaagga aaagatgaaa ccgtagaagg agaactatta gccatttcag ataaggttga
    47761 ccttctttat gaatcttttg atgaaatcat caaaaataac ccggaaaaag tttataagga
    47821 gatgttcatt gaggcagtga ctacgattaa aagctttgaa catcggcctt ctgttcagta
    47881 tttctttaaa gagatatttc cggaactatt gaaccaggaa ttttatggaa aagaggcgtt
    47941 tttagaaacc gtctcaacga ttgtaaagaa agaagaaaaa taaaaatgat atgagatgca
    48001 gggggaagta tagttgaaaa agaatatgga tgtcgaatgg ggctattctg gagacaaagg
    48061 tccggaaaat tgggcgagtt tatgtgattg gtttgcgaga ggtgcggaat ttccattgca
    48121 gtcgcctatt catttgacca agggggaaga aaccaaaata gaaggagaca cgctttcctt
    48181 tcattatcaa aagcaacggt ttactgacaa ggaattcaaa aacacgattc atttagtacc
    48241 ttttgaccaa ctcagttatg tagagttcaa gaaagaacgc tattatttga cagatattca
    48301 tttccacttg ccttctgaac atatcatcaa cggaaaacag gaagatattg aatttcattt
    48361 tgttcatatg aacagcaaaa aagagaatct agttgtgggt gtcatgtacc agctaatggt
    48421 acaggaaggt tggctgtata atcaagaaaa tggggaaagt tggaacttag aaaaacatga
    48481 gcattgggta aatccggatg tgttctttcc ggagcaaaaa agccatttcc attatattgg
    48541 ctcattgaca actccgccta ccagcgggcc aatccaatgg tttgtaatgg atcatgtagg
    48601 aaaactgaac caagagtttc ttctcccctt cgatggtcag tatgctcgac cgaacaaccg
    48661 accaatccag cccttgaatg gaagagaaat ttattatttc gaggagtttg agcaatgaat
    48721 attcagcaga tgaaatacgt ggtcgctgta gcaaacaatg gtagtttcag agaagcggca
    48781 aagaaaatgt ttgtctccca gccaagtctt tcagctggta tcaaagaatt agaaaccgag
    48841 ttagggatca cgctgtttac tcgaacgaat cgaggggcat atttaacaga agaagggcaa
    48901 gacttcttaa atcgtgccga gaaaatattg gtccagcttg atacattaga aactcactat
    48961 ctaacgagtg aaaagagaga caatttttcg atcgctgccc agcattatga tttcttaggt
    49021 cctttgaccg cacagctact cgaggaattc aaagacgaga tcaaacaatt tcggatcatg
    49081 gagacgacaa cagcgagagt gatcgaagaa gtcaaagagc agcacagtga gatcggcatt
    49141 atttatatga atgagcataa tcaagctggt atcagaagat atttacaaca aggagagcta
    49201 agtggccgca gcattggaaa attcaaaacg catatttttt taaggcaagg acatccatta
    49261 gctgaacacg cagagatcac caaagaagaa ttgttatctt atcctcaagt tcgttttaca
    49321 caggatggaa ataatttccc gtatttttat gaagacctta tcgagatccc tgatcaagaa
    49381 acagtgattt atacgagtga tcgaggtact ttgatgaata tcgttcttga aactgatgct
    49441 tacgcttcag gatctggtat tgtgattgga gggattcgcg agcacttgcg gttaatccct
    49501 ttggcagaag gcgaactaaa tgaattttat atcatttatt cggcgaaacg tccgctcagt
    49561 gaaatcgctc aaagatttat caaagagctt actcggttac tggaccagca gagctaagaa
    49621 aagcagatga gaacgctgtt tattgaacac cgttgatcaa aaaagagggg ttgtgacaga
    49681 attttgtcac aactcctctt tttgtagcaa acaatttata actaattgct gttgtgggaa
    49741 agtacgcaaa tatgattcta gaaacactca ttgattacct gttttgtttc atagctgtaa
    49801 ttttttcttc ttctggtgtc acgataatcg tcttttggtt ttcataaata acataaccag
    49861 gcttggcgcc atttggtttt cggatatgct ttacctgtac aagatcaacc ggcacttggg
    49921 cagagtatcg gtattttgaa aaataggcag ctaactcggc agcttctgta attgtttcat
    49981 ctgagggctt gtcactttta atgatgacgt gcgaaccggg tatatttttt gcatgaagcc
    50041 aatagtcggt ttttttagct gtcttcattg tcagctggtc attttgaaga ttgtttttac
    50101 caacaagaat gagcgttccg tcagaagaaa agtattgatc aggctgagat ggtttcttac
    50161 gtttttgttt ttttaagttc ttctttttca ggtatccttc tgaagtcaat tcttctttaa
    50221 ttgcttcgat atccattggg ccggcaattt ccagttgaga taagacagat tcaagatatt
    50281 ggatttcatc tttggcttct tggatttgtt cgccaatcag cttaacagcg ttcttcagct
    50341 tttggtatcg atggaaatat ttctgtgcat tttgatttgg tgtcaatgcc ggatctagtg
    50401 ctattttgat tggccgatct tcttcataat aattaggtaa aacaacttct tttgcgccgc
    50461 gtggtacttg tgtcagaaaa gtagtaagta gttcgccatt tctacggtaa ttctcagcgt
    50521 tttcggattc tttgagtgtt tgttcacgtt ttttcaattt atttttattt cgttttagtt
    50581 cattttcgat ttttcgaatc agttctccgc cttgttgttt tgcccgatct ttctctgctt
    50641 tttcgtgata atagctatct aataattgac tgagtgttgg ataagctgtt acagcagatg
    50701 cctgttcgga caatacctga taagcaatcg gtgtgaaaaa atctttattt tccgtttcgt
    50761 aaaaagtagg aataggctga tggctgatgg ctgagaaaaa ttcgtcccat acaaccatct
    50821 tttcattagg acgttccagt aatcttttgg tcagctcttg agcgatatcg aaaccgattc
    50881 cctgaaactg acgttggatt gcttttggat ctagttcagt ttgggaaagc cgctgaaaga
    50941 ttttttcttt ctcggagcta aatgggttca agacctcttg ttttggcgga gcaacatatt
    51001 ctacaccagg aagcaaagaa cgataagtgt tttgagagct tccaatgtgt ttgatcgcat
    51061 ccagtatttt tccagtttct cgattaacta agacgatcgt actatgacgt cccattaatt
    51121 caacgatcaa tatgatattt tgtagatcac ccaactcgtt tcgtttagca aagtggaaat
    51181 ggatcactcg atcattttcg atttgttcga tgtcttctaa aatagcgcca tccaaatatt
    51241 ttcgaagcat catcacgaag ttaggcggat tatcaggatt ctgataatcg atttgggtaa
    51301 tctgtacacg agcataactt gggtgcgcag ataataataa tcgatggttt ttgccgcgtg
    51361 aacgaatcac cagcacgatt tcattttcat aaggctgatg gattttcgag atccggccag
    51421 atagtaacgt ttcacgcaat tcgttgacca tcgcgtgggt aaatacacca tcaaaggaca
    51481 tggttcattc ctcatttctt ttcataagta tattcattat aacgaaaaaa agaagaaaaa
    51541 ccaatttggc cgaatagtaa agcaaaagtc tagaaaaatc ttggactaat tacataaaaa
    51601 acagtgtttc tatcgttgaa atccaaattt ttaggt
//
LOCUS       DARBEG010000010        51238 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000010 DARBEG010000000
VERSION     DARBEG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.010"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            290..1135
                     /locus_tag="SCK20_000581"
     CDS             290..1135
                     /locus_tag="SCK20_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1144673.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1416..2636
                     /locus_tag="SCK20_000582"
     CDS             1416..2636
                     /locus_tag="SCK20_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG1144674.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2633..5353
                     /locus_tag="SCK20_000583"
     CDS             2633..5353
                     /locus_tag="SCK20_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1144675.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5337..6281
                     /locus_tag="SCK20_000584"
     CDS             5337..6281
                     /locus_tag="SCK20_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1144676.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6350..7360)
                     /locus_tag="SCK20_000585"
     CDS             complement(6350..7360)
                     /locus_tag="SCK20_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1144677.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7540..7884)
                     /locus_tag="SCK20_000586"
     CDS             complement(7540..7884)
                     /locus_tag="SCK20_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1144678.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7877..8311)
                     /locus_tag="SCK20_000587"
     CDS             complement(7877..8311)
                     /locus_tag="SCK20_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG1144679.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8449..9186
                     /locus_tag="SCK20_000588"
     CDS             8449..9186
                     /locus_tag="SCK20_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144680.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9179..10390
                     /locus_tag="SCK20_000589"
     CDS             9179..10390
                     /locus_tag="SCK20_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144681.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10594..11376
                     /locus_tag="SCK20_000590"
     CDS             10594..11376
                     /locus_tag="SCK20_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG1144682.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11446..12108
                     /gene="trmB"
                     /locus_tag="SCK20_000591"
     CDS             11446..12108
                     /gene="trmB"
                     /locus_tag="SCK20_000591"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG1144683.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12206..13960)
                     /gene="ade"
                     /locus_tag="SCK20_000592"
     CDS             complement(12206..13960)
                     /gene="ade"
                     /locus_tag="SCK20_000592"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG1144684.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14092..15021
                     /gene="tadA"
                     /locus_tag="SCK20_000593"
     CDS             14092..15021
                     /gene="tadA"
                     /locus_tag="SCK20_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG1144685.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14978..16027
                     /locus_tag="SCK20_000594"
     CDS             14978..16027
                     /locus_tag="SCK20_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG1144686.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16024..16302
                     /locus_tag="SCK20_000595"
     CDS             16024..16302
                     /locus_tag="SCK20_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG1144687.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16277..16747
                     /locus_tag="SCK20_000596"
     CDS             16277..16747
                     /locus_tag="SCK20_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG1144688.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16764..17066
                     /locus_tag="SCK20_000597"
     CDS             16764..17066
                     /locus_tag="SCK20_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144689.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17083..17454
                     /locus_tag="SCK20_000598"
     CDS             17083..17454
                     /locus_tag="SCK20_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG1144690.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17426..17785
                     /locus_tag="SCK20_000599"
     CDS             17426..17785
                     /locus_tag="SCK20_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144691.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17957..18964
                     /locus_tag="SCK20_000600"
     CDS             17957..18964
                     /locus_tag="SCK20_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1144692.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18989..20173
                     /locus_tag="SCK20_000601"
     CDS             18989..20173
                     /locus_tag="SCK20_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG1144693.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20610..22193)
                     /locus_tag="SCK20_000602"
     CDS             complement(20610..22193)
                     /locus_tag="SCK20_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1144694.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22771..22914)
                     /locus_tag="SCK20_000603"
     CDS             complement(22771..22914)
                     /locus_tag="SCK20_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG1144695.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23136..23615)
                     /locus_tag="SCK20_000604"
     CDS             complement(23136..23615)
                     /locus_tag="SCK20_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1144696.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23688..23972)
                     /locus_tag="SCK20_000605"
     CDS             complement(23688..23972)
                     /locus_tag="SCK20_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144697.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24050..24244)
                     /gene="ssrS"
                     /locus_tag="SCK20_000606"
     ncRNA           complement(24050..24244)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SCK20_000606"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24316..25602)
                     /locus_tag="SCK20_000607"
     CDS             complement(24316..25602)
                     /locus_tag="SCK20_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG1144698.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25740..26411
                     /locus_tag="SCK20_000608"
     CDS             25740..26411
                     /locus_tag="SCK20_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG1144699.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26377..26865
                     /locus_tag="SCK20_000609"
     CDS             26377..26865
                     /locus_tag="SCK20_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG1144700.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26878..27825
                     /gene="prmA"
                     /locus_tag="SCK20_000610"
     CDS             26878..27825
                     /gene="prmA"
                     /locus_tag="SCK20_000610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG1144701.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27826..28590
                     /locus_tag="SCK20_000611"
     CDS             27826..28590
                     /locus_tag="SCK20_000611"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG1144702.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28823..31036
                     /locus_tag="SCK20_000612"
     CDS             28823..31036
                     /locus_tag="SCK20_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG1144703.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31047..31493
                     /locus_tag="SCK20_000613"
     CDS             31047..31493
                     /locus_tag="SCK20_000613"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG1144704.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31664..32923
                     /locus_tag="SCK20_000614"
     CDS             31664..32923
                     /locus_tag="SCK20_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG1144705.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33012..33959)
                     /locus_tag="SCK20_000615"
     CDS             complement(33012..33959)
                     /locus_tag="SCK20_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1144706.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34353..35645
                     /locus_tag="SCK20_000616"
     CDS             34353..35645
                     /locus_tag="SCK20_000616"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG1144707.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35660..37429
                     /gene="aspS"
                     /locus_tag="SCK20_000617"
     CDS             35660..37429
                     /gene="aspS"
                     /locus_tag="SCK20_000617"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG1144708.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37538..38413
                     /locus_tag="SCK20_000618"
     CDS             37538..38413
                     /locus_tag="SCK20_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1144709.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38679..40562
                     /locus_tag="SCK20_000619"
     CDS             38679..40562
                     /locus_tag="SCK20_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1144710.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40637..40921)
                     /locus_tag="SCK20_000620"
     CDS             complement(40637..40921)
                     /locus_tag="SCK20_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1144711.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40911..41645)
                     /locus_tag="SCK20_000621"
     CDS             complement(40911..41645)
                     /locus_tag="SCK20_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG1144712.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41774..42397
                     /locus_tag="SCK20_000622"
     CDS             41774..42397
                     /locus_tag="SCK20_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG1144713.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42589..43710
                     /locus_tag="SCK20_000623"
     CDS             42589..43710
                     /locus_tag="SCK20_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG1144714.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43717..46845
                     /locus_tag="SCK20_000624"
     CDS             43717..46845
                     /locus_tag="SCK20_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG1144715.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46864..50058
                     /locus_tag="SCK20_000625"
     CDS             46864..50058
                     /locus_tag="SCK20_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG1144716.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50204..50911)
                     /locus_tag="SCK20_000626"
     CDS             complement(50204..50911)
                     /locus_tag="SCK20_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1144717.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16770 a         8945 c        10634 g        14889 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaat ctcagaatta taaaaataaa atgaaaaaaa tttttcacga
      661 ggtagggagt gaattttcta tcaaagagct aaactatgag cttgagtatg aaaaatctat
      721 ttgttcaatc ccttataata atattgagga aattatagaa aaggatgcta tcagcattta
      781 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
      841 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      901 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      961 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
     1021 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
     1081 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
     1141 ttaaggtagt atagaacata gaattctaaa tacggaaaaa tggagcataa attccagtac
     1201 aaatagtact tcttcagtgt caaacgaagc gaatataaca tagatattta ggattgtgac
     1261 aaaaatcatg tcacaatctt tttttcgaat aaacttattt tttgaagtcg gccatttatc
     1321 gtaatttctt tgcataatac gaacaaattt tcggttattg atgaaaaaaa acattgaaaa
     1381 tggtatgata aaagaaaaaa aggaggaaat ttttaatggt gaaatttatc catgcggcag
     1441 atttacatat ggatcggtca tttgaaggac tgacaactct tgataaaact gtacaggaaa
     1501 aactattgaa gacgaatctg accgttttat caaatatcat tgaacaggca ataataaata
     1561 acgttgattt tgttttactt gcaggagata attttcatca aaatcgtcct tcattaaaaa
     1621 tacagaaaca tttttcagaa cagatgaaac ggttagaaaa gaaccatata cctgtattta
     1681 taatattcgg aaatcatgat ttctatcaaa aagagcggta ttggttctcc tttcctaaaa
     1741 atgtccattt atttacatcc gaaacggtag agacaaaaaa aataacgata aaaagtggag
     1801 aaactgtctc tctcagtgga tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga
     1861 tggaatttcc ttctagagaa ctcacagatt accatattgg gttgtaccac ggggaacctg
     1921 gcgtctctca aaaaggaaac tatgctcctt tccaaccctc aaagatgcaa gaaaagggat
     1981 acgattattg ggctttaggg catatccatg tgccaacggt cttaaatgaa aagcaaacaa
     2041 ttgtttatcc aggtgcgcct caaggtcata ctaaaaagga acaagcttct acatctattc
     2101 tactagttga attaagtaaa ggaagttgcc aaatacatcc gattaaagta gcagaggtct
     2161 attggaagac aaaggaaata tcattgagga aagcaagaac gacaaaagag gtcattgttc
     2221 acattcgtca gcagctttca aaagtagagg atggatttag tttaattgag ataaaattaa
     2281 aagattatga ccatctcaat acagatgttt tggaacggat acaatcagga gagctattag
     2341 actatttgtt agaggaattc tcagatagga taaatgattt ctttatttgg cgtatttctc
     2401 tcatagagaa tactttttat acgaagaccc cacttgctgc ttctgctaaa ttgatggaac
     2461 agttatttca atactatcag acacctcaag atttccaaca aattttaaat gaagtatata
     2521 gtcatcaaga agcggctaga atattgagcg aactgccgga atatcaagaa gaaacacttg
     2581 aaaaagccaa gcagctgctt aatcaagatt ttttatttga ggaggatcag gaatgaagat
     2641 tttaaggatc gagattgctg cttttggaaa atggcgtcaa aaaagttttg acttttattc
     2701 aggcaatcag ctgatctatg ggggaaacga agcaggaaaa tcaacgattt atcaatttat
     2761 tcaagcaata ttatttggtt tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa
     2821 agacggctca gcctatggcg gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc
     2881 tgtcgagcgc tacaagcagc aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt
     2941 gggaagtgat gagttactag aaaaactgat cgcacctttg acgaaagagt tattccagca
     3001 ggtatttact ttccagcaag agcagttgat ggaactagat catttgagag aaaaagagct
     3061 acatgatgcg ctgatttctt taggcatcac gggcagttct caagtattcc aaaagcggca
     3121 agaatattat cagaaagcac aacaattatt caagaaaaaa ggccaaaaat tactaatcaa
     3181 tcaaaaatta aatgaatgga aagagctgca agaaaaaatt catcaaaaac aagaacagga
     3241 agcttatttt ggcaagttga tccaacaaga acaggaatac gagtcgcaac agcagcagtt
     3301 attggcacaa ttaaaagagg cgaacaaaca attttcatta gccagacagc aagaacttaa
     3361 tttttcttta tatgaagaat ggcaatctct caaaaaaaac agcactacag gtcttcctga
     3421 tgagcaactt cttttagatc tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg
     3481 tttggaagaa ttagaagcaa cactggaaaa gcatagcggg atggatcagc agtcagccag
     3541 atattatttt tatttagagc aggagcagga actaaaagat ctccagcaac ttcaatttac
     3601 agcggaacaa ttaaacgaag aatggcaaag attacaaatt gagactagag aaacaaattc
     3661 agaattgaag attttggaac aaaggtggca ttggcagaaa aatccgttac cggaagaatt
     3721 tttatatgaa gaagaatgga agcatcttct ggctgaagaa agaaggattg ctgatctaga
     3781 aagccagatt tctgtgcgtc tccaaatagc taaagaacag caagagatga ttgaagcaga
     3841 agtgaatgac ttggaagaga cctatccagc attattacaa aatcagtcag agatagcacc
     3901 agtctcttca tctaaaccgt atatgctgat ggctggcgga gcaataatca ttgcaggtat
     3961 atttgtacca atgcccttga aagtgatcct tttactaatt ggtgcaggca tgattggcta
     4021 tttcttctac aaaaggcaac caaaaattaa aactaaaaat gatttctctg agataaaagg
     4081 aatgtggcaa gaaaaactag gacaacttga tctagcctta gctcaattag ctgaagcaga
     4141 agaaaaacaa aagagaactg gagaagaaaa acagattctt cagcaagaag tatatcgttt
     4201 tgcggatcat cggcacttag gaaagatgga tacgctcaaa ctcatgaaag aatatggaca
     4261 ggaagtcata gattatcaga aattatatgg cgtatttgag cggaaacata aaagagaaca
     4321 ggaaatacaa gaaaaactac gacaaatcga ccaaaaattt gagtttgtta aagaatggtt
     4381 accgatacaa gataagacaa tttcagaaaa aatggtattg ctttcccgtt ttatacaaga
     4441 gatggaacag atgaaatttg ccagaagcta tcagcaaaat actttattga agcaagaaat
     4501 caatcaattg aaaaagaaac aaaaagtatg gatacaagaa tacagcgaac aattgactca
     4561 agcaggctta tcttatcctt cggaagtacc tgtcttttta caaaaagcca aacaagccga
     4621 acagcagatg aaaagataca atgaattatc ggaaatgctt actccactat ttcctgaaga
     4681 gctgacaaaa gagaaattat ctgtaagact gcgccaactg aaagaaaagc aatcccaact
     4741 ccagcaagaa gaaaaagaag caagtgaaaa tcggcaacga ctccaacttc aagtagaaga
     4801 gttgcaaaga gacggcacat tggacgaact ttatcaacaa gctagccggc aaaaagcaaa
     4861 attacaggaa ttactggagg aatggggagc acttgaaatc gccgggacat tcttaggtga
     4921 tctttcaaca gagatgagtg aaagacaatt accccagttg ttaagagcag caggtgatta
     4981 tttaggtatt ttaacaaaag gaaattatca gaagatatta ctgaaaaatg atacactgac
     5041 tttaacagac ggatatacag aatttcccat ctatgagtta tctacaggta cgaaagatca
     5101 gctgattatg gcgatgagat ttgcttacct ctcattagaa gctaaccgtt cgatatgccc
     5161 gatcatcatt gatgatggct ggcttcatta tgatcatcag cgaaagtatc aattagctga
     5221 acttttagct aaatttggag aaaagtatca aataatttgt ttttcttctg ataaagaaat
     5281 ggtaagctat taccatgaga tgaaccaaat cgtaaaacaa ttagaaggag caaataatga
     5341 aaaaaattcg tgatttagta atcgatgagc agtttgaaca atttgtcctg atcaaaagtg
     5401 cagatgtaag aatcgctaaa aacggaaaaa aatttattgc ttttactttc caagatactt
     5461 caggtactat cgatggtaaa tactgggatg cttcggagga ggaaatcaat aaatttacag
     5521 ccggcacagt cgtttttcta aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa
     5581 agattcttca tatgcgctta acaagggtag atgagccagg agagcctagt ttatacatgg
     5641 aaagagcccc tttgaagaaa gaagaaatgg aagaagaaat caatcaattg ttgtttgaga
     5701 tcaccaatgc gcattggaat cgtattgttc gctatctact taataaatat caaaaacaat
     5761 tttttgagtt tccggcagca aaaagaaacc atcatgcatt tgccagtgga ttagcttatc
     5821 atactttgac aatgcttcac ttagggaaat caatcgccga tgaatatcct gagctgaatc
     5881 gagcattgtt gtatgctggt atcattttgc atgatttagg aaaggtgatt gaactttccg
     5941 gtcctatgtc gacagaatat acgcttgcag gaaatttgat cggtcatctg gtattgattg
     6001 acgaagaaat aactaaagcc tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc
     6061 ttgtattacg tcatatggtt ttagcacacc atggcttatt agaatatggc tcacctgtac
     6121 gtccaagaat aatggaagca gaaatattac atcaaatcga caatatcgat gcttcgatgc
     6181 agatgatgtt gacttcgatt cgtcaaacag aaccaggaaa atacaccgat cggattttcg
     6241 gtatggacaa ccgaagtttc tatgtaccaa aagatttata gcaaaaattt atttttttca
     6301 ttcaaaacaa aaaaagcgag acataagtct tcgctttttt tgttttcttt tattttgatg
     6361 aatcagttgt ttcttcagtt gaagattctt ttgttgaatc agttgatttt gtgtcagaac
     6421 tctttgttga agctgtagtt tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg
     6481 cactcaacac attttcaaag gcatcatctt tgatttttac atttgcatct tttaattctt
     6541 caccgattac tttagttgtg aatgcattgt cacttagttt agtttcagta gcgatatctt
     6601 ttagttgatc tttgtattta tccatgtcat tgcctttatt ttggtttttc accattttga
     6661 caacgtagta ttctgtcgca taagaagtag gatttgttgt agtaataaca tcggagatct
     6721 caccgtcttt caatttgaaa gctgcttctt ttacttctgc aggaatagtt gttgtcgttg
     6781 agtcgaattt aactttaccg ccatcttctt ttgtctctgt atcagttgat ttgtctttgg
     6841 ctaatttaga gaaatcatct ccatcatccg ctgatttttt cacatctttt gcttcatctt
     6901 cacttgataa tttgatgatt tgtgcttcta cttctggatg gaaagatttc caagcagttt
     6961 tcaaatcgtc atctgtaata tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt
     7021 tacgaatata ttctttataa gtatctttcg tatatccagc agcttctaat tgtgattcaa
     7081 aagtatcacc taatgatttc gcttgtttgt cgtattctgc atccacttgc ttatcggtta
     7141 ctttatcgcc atatttgtta ttgaatactt tataaatgat catccgttgt actaaggatt
     7201 ggttcgatga ttcaagtttt gcttcatcat agaaatcaga tacagtgatc gttccgcctt
     7261 tcatcgttgc gatatcttta ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca
     7321 acgcactagt agctgccaaa ataattgatt tttttttcat taaaatttgc actcctgtaa
     7381 ttagttatta tttttatata ttaactatac catatcccat ttcctttaaa aacgttgtcg
     7441 tggtagtttc aaacaaaatt caaaatttca tcacattcct aggaaatacg caaacttttc
     7501 tcacttatat agttaaatct gttgagaaaa taatcattat tatatttgtt caggatactc
     7561 tggcagatca gcttttagtt tttctacttg ttcattgatt tgtgtgatac gtggttcggc
     7621 ttggaattta aaatcttcga tatctttttt gattgcttct tggaaagcag gaatcacaga
     7681 ttcagctgtt tcttttgttt cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa
     7741 atcatttgtc agagttgttg cttcttccaa ttcttcgatc agtttctttc ttgtttcatt
     7801 cccgctcctg ggcgccaata acagacctcc gacagtgcca gcagcagctc caaataacag
     7861 tcctttgaaa aagtttttaa tcatcgcagg acacctgctt tacgattgtt tcttgaatag
     7921 cttttagttc ttccgatcca taactatctt gatgattgac aaagtgaata gaaaaatcat
     7981 cctctttgct gtagcgaggg actaaatgga tatgtgaatg aaaaacagat tgataggcta
     8041 gttcacggtt gttattcaag atattaagac ctttcatttc tggaaaagct ttttccaatg
     8101 cttgagcaat cttaggcaca cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa
     8161 aaatatctgc tacatgacgt ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga
     8221 tgtctaaaaa agcatagacc ttgtcgtctt catatatttt atagcttggg atttcttgat
     8281 tgatgatttt gcaaaaaata cagttttcca tataaaacat cctttctata aagaataaat
     8341 acttcagctt aactttacca taagttaaag ctattgtaag gaaaaaaaca aaataccgtc
     8401 attttcttcg tggtataata aataaaaata aatggcggag gaaaaagaat gagtttaatt
     8461 atcgatcatg tcacaggggg gtacggacat ctccctgtgt taaaagatat cagtttcgat
     8521 gtacagcctg gagagatagt agggttgatt ggactgaacg gagcagggaa aagtacgacc
     8581 atcaagcaca ttatcggtct gctggaagct tcaaaaggaa cgatagaaat caatcatcta
     8641 tccttaaaga aaaatacgga agaatatcgc aaacaaattg ggtatattcc agagactcct
     8701 tctttatatg aagaattgac attgagagaa catatcgaag tcactgcgat ggcctacgat
     8761 atacctatgg atatagcaat gaaaagagca gaaaagttgt tggagacttt tcggttatct
     8821 aatcgtcttg actggttccc agctaatttt tcaaagggaa tgaaacagaa agtgatgatc
     8881 ttatgcgcct ttttgatcga gccttcttta tatatcatcg acgaaccatt tttaggcttg
     8941 gatccactag ctatcaatgc attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc
     9001 ggtattttga tgtctacgca tattttagct actgcagaaa aatattgtga tcgttttgtg
     9061 gtcttacata acggagagat acgagcagac ggttctcttg agatgttgag aaaagaattt
     9121 cagctgccgg attcttcttt ggacgaaatc tatattgctt taacaaagga gcaggcacat
     9181 gagtagtttt tttcaaaaac gtttagcaag acatcaaaag aaaatgatga aatatatgcg
     9241 ttatgtattg aacgatcatt ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt
     9301 atattattct aattggttga aaaccctgca gccaccgttt gcaattggag ggctcataat
     9361 ctctgtattt tggatgctct gtttattcgt gggtaagatg gctacttttg cagaggcagc
     9421 agatattgtt tttttacttc ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt
     9481 tcgctattct tgcatttttc cgttgatctt tttgatattg gcatgcggat tggccatgcc
     9541 attagtggtg gttagtacgg agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt
     9601 atggagtttg aaattcagtc atctgcaaat caagagagca gaattgtttc gactagatca
     9661 acaagttatt cgttattgga aattaggctg gtttttttct tctctgatct ttttgtttct
     9721 atcattttat tggctaatat gggcaggaat agcaggtgca ttagtacagg caagtctata
     9781 ttactggttt ttgtggaagc aacaagaaaa aggattggat tgggagaaaa tggtgcatgt
     9841 agaagaacat aggttgcatc agatctatca atttatcaat ttattcacag atgttcctga
     9901 aattacggca aagatcaaaa gaagacgcta tttggatcct atattgactc atattaaaag
     9961 agaatcaaaa aacacttatc tttacttgtt tgcaagaaga ttggcaagag gttctgattt
    10021 tagtggtctt tatttccgat tagtaattat aggtgggtta ttgattgccg gagtagtcga
    10081 cttttatttt agtctgatta tcggtttctt atttttatat ttgatcggtt tccaactgtt
    10141 acctttagcg aaccagttcc gctatatggt tctcgttcag ctttatccaa tccataagaa
    10201 tcagaagaag caggcgattc agtatctact cacatgggta ttaattatcg cgtcggttat
    10261 ttttggtttg attgctgcag tcgtattaga tgggcagcag cgatgggttc cgctagcggc
    10321 ttatttgatt gttactatgc tctttgttaa actatatgta ccaactcgtt tgaagaagat
    10381 gtcagactga aaacaaagct gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca
    10441 gtaagaatgc tgttcgttct attttttttg agaaaagctg gttttttgaa tggcaattaa
    10501 atgaaaaaga acgtatatgt gcttgacgtg tagtgttcag gcgactagaa taataagtac
    10561 tgcaaaacaa agagtaagag gacgggagaa tgcatgatgg agtttcagtt agaccaagag
    10621 tggcggttac gtcccatcaa aggcgacaca ggaaaaacat acataggcat gcgtgatgag
    10681 gacaaagtct ttattaaacg aaacacgact ccgatgttgg ctgctctttc aagggaagga
    10741 attacaccaa aacttgtttg gacaaaacga acggggaatg gcgatacttt gacagcacaa
    10801 gagtggctgg aaggtcgctt gctgactcct aatgagattg gtcaaagaaa cgacgtgatt
    10861 gatgttttgt accatttaca tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt
    10921 ggtcgtgtca tgacgccgga gaagatgctg caaaaatatg aaaaagaatt accggaagaa
    10981 ctccagaaaa atcaatttct gcaaattgtc catcgttttc ttcgtgagca gatcccgtca
    11041 atagataagg aaaacttatc agtagtacac ggagatgtaa accatcgtaa ttggttagta
    11101 tgtaaaaatt atttatatct agtcgattgg gattcaatca tgtttgcaga ccctgcggtg
    11161 gatattgcaa cgattttagg aaattatgtg ccactgtcta gctggaatca gtggctgatc
    11221 agttatggca tacgcccgac tgacgaaatg cttgaaaaat ttcattggta tgcggccatg
    11281 aatttgctcc aagaaatcac cagatattgc aaacgtgaag atcatcgacg gatgaacgaa
    11341 gaaattttac aattaaaacg aattttcagt ggctgaggtt tggcccaaac tctgcggaga
    11401 tttggccaga cctttttaat tagaggaaag gaatggagat aaataatgcg tgtccgaaat
    11461 cgtccgggag cagcggaaat gttagcggct cacccgaatt ttgtaattag tgatccaaca
    11521 ttgtggaagg gaaaatggaa tgaattgttt gaaaacgatc atccaatcca cattgagatt
    11581 ggtatgggga aaggccagtt catcactgga atggcaaaag cccatcctga aatcaattat
    11641 attggagtag aaatgcaggt cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa
    11701 ccgcttccta acctgaaact tctacatgtg gatggatcag cgttgacaga gtatttcgct
    11761 gacagcgaag tagatcaaat ctatctgaat ttttccgatc catggccaaa aaaacgtcat
    11821 gaaaaacggc gattgacata taagactttt cttgctgtag atgaacagat tcttcgtcca
    11881 aatggagaaa tccatttcaa aacagataat caaggattgt ttgagtattc tctggcaagt
    11941 ttttctcaat atggcatgat cctcaaacag gtatggttag atttacatca aagtcaattt
    12001 gaaggcaata ttatgacgga gtatgaagaa aaattttctt caaaaggtca acgtatttat
    12061 cgagtagaag ctcaatttca agataagacc aaaaagaaaa gtctgtgaaa ctaactttct
    12121 gctttcataa aaacattaat atccataaat aaatctcaga aaatgaacat tacagatgta
    12181 atgtattttc tgggatttat ttgatttaaa atgtatttat ttcatcggtt tttccacaag
    12241 gtggaatcaa ttggaagcga gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg
    12301 gtatgacaga taatgttaag aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc
    12361 ctagttgatg tgctttagtt attaattcat tcaactgttc atagacgatc gaaaaaggtt
    12421 cctgtgtcat gattccgcct ataggaagag aaacacaagc tatttcctgc tgtttgttga
    12481 caacggtcat cccgccacct ttttgaatca aaagattagc agcatacagc atatcctcgt
    12541 cgtttgtccc aacgacaact agattatgcg agtcatgggc aatcgttgtc gctatcgctc
    12601 cttgtttcag ctgaaatcct ttcactatgc ctaatccaat gtttcccgtt tgatggtgac
    12661 gctcgatcac cgcaatcttt aattggtctt tttcgattga aggaatgaat cgttcgcctt
    12721 gtcggtccac tttttctacc aaatctttag tcactagact attcgggata atttcgatca
    12781 catgggcttg tgaagaagaa agaagcagct ctaacgaatg tggatttaat ggttgagcgt
    12841 ttagttttgg cagacaacac gtatatgaat ttgaatgatc agcaaataaa tggtcttgga
    12901 gttgcccaga ttgaacaact aatgttccct tatagaaaac ttcatgaatt ggcagcttgg
    12961 taagatcgtc tgttaagaag aaatcagctt gatacccagg ggcaatcgca cctagtgttt
    13021 tcaaatcaaa acattctgct gcattcaatg ttgccatctg aatggcttgt aatggatcga
    13081 ttccaagtgc aatagcttga cggacgatat gatcgattga tccttctttt actaaatcat
    13141 tgatcagttt atcatcggtg acaaagaagc atcttcttga attttcaggt gtgatcgccg
    13201 gtagtagcga tgccagatct tttgcaacgg tcccttctcg aaccattaaa tacataccaa
    13261 gatccaaacg ttctttagct tcttttgcag ttgtagcttc atgatccgtc cgaatacctg
    13321 ctgcaggata aacatttaac tcttccatgc cgataccagc tgcatgcccg tcaatttttt
    13381 tcttatgctg gtgcatcaat tggatctttt ctaggatgtc tgtttcatta tttgctactg
    13441 cttgatagtt catgacttca gctaagccta aaacttcggg tttttgtaaa aaaggggcta
    13501 atttttttgc atttaaattt gcaccattgg tctcaaaagg ggttacaggt acacaagaag
    13561 gtagcataaa aaagatattc atgagtgttt gtcttgcatc ttctatcata aactcaagac
    13621 ctgtactccc agcaatgttt cctatttcat ggggatctgt cactacagta gtaactccat
    13681 gaagcaagga tacttttgaa aattcgcttg gtgataaaag tgagctttcg atatgcacat
    13741 ggccatcgat gaatcctggc actagatatt gcccattagc attatgtacg ctttttcctt
    13801 cgtattcacc gattcctacg atcattcctt cgcagattgc tacatctttt tccatgattt
    13861 ctttcgtaaa gacgttgacg atcttagcat gcttgatcac taggtcagca ggcttttttt
    13921 tacttgctac ttcgatatat ttccttaatg ttgtttccaa tggagtgcct cccaagataa
    13981 atttttataa gtataacata aattcggctt ttgccaatat ttttttgtaa attcatttta
    14041 tttgaaacaa aaagatcaaa aagacgtatg ttttttgtgg aggggagaat aatggaaata
    14101 aaacgattat cggatcaaat gataagtgaa agttttgcca aacatgccca agacgtatat
    14161 atattgccag cttcagaagt attttacatc tattttcgct atggaaaaaa acgaacattc
    14221 tggaagaaag tctcttcggc agatggacag caactgatct caagatttaa gtatttggga
    14281 cagatggata tcggagaaaa aagaaaagct cagttaggtg catttactta ttctttagaa
    14341 aaacaggaag ttcgtctgcg gttgtcttct gtaggagatt accttcaacg agagagttta
    14401 gtgattcggc tattatacgg tacttttgaa acaccccatc gttgctttag ttctcgagat
    14461 tttgcaacta ttttacagaa aaccgtcaga cgtggattat accttttcag cgggacagtc
    14521 ggatcaggca agacttctct aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt
    14581 actatcgaag atcctgtaga gatagaagaa ccgttttttc tgcagctaca gatcaatccg
    14641 aaaatagcac agacttatga tacacttttg aaactcgcat tacggcatcg tccagacttg
    14701 ctaatcattg gcgagataag agatgaatca actgcacatg ctgcggtaag agcagcacta
    14761 acagggcacc gggtttttgc aaccgtacat gcacgcaatc ttgaaggtac attggtcaga
    14821 atccatgaac tgacaaagaa aaaagaagag ctatctgaat gtttagctgg tgtcatttat
    14881 caagaacttt tggaagatgc caatcaccat ccttctgttt tatggagtta tcaatttatg
    14941 gaggaagaat cagtaaaaaa atcatgggag gaaagttatg aagaagctca ggaaaatatt
    15001 tggcagtcag aactattcta aaggattaac aagaagccag caaaaccagt ttgcacaaac
    15061 catgggagaa ctgcttttat ctggttttag tctgcaagaa tccctttatt tattactcac
    15121 gatccgttcg tttccacctg ttattttggc aaatatccaa catcagctaa aggaagggca
    15181 agcgtttagt gaggtacttt cgcaaatagg atatggagca gagcaactgg tacagatcga
    15241 gttagctgaa cgtcatggga atctgccgac aactttgttg gaaattgcca aacagtttcg
    15301 gcttgttgaa ggatttcgca atgatttgaa aaagctgctt gcatatccga tgctattgct
    15361 cacctttttg ctaggtatct tgtttagtat gcggttgttc gttttgcctc agcttattgg
    15421 ttcaggaatg gtcatgcaag agcattgggg tattcagctg atacaagtct ttcattggta
    15481 tgttctacta ttcatcctag cggttactct attggcttta ctagtaaggt atcgtctgat
    15541 gaagttgtct cctgttgatc agagtgaatg gataagcaga caattattga ttggaccgat
    15601 atattccttg tatcaatcgg cttatttgtc actagagatt ggaaagcttt ttcatgaagg
    15661 tttcgaatta aggcaaatca tagtctgctt aaaggaaacg aaacaagaaa gtctgattca
    15721 gcatcttgcc tctaagttga ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt
    15781 ccagaattat cgttttctat cagaagaatt cagtaaaatt gttttacagg gagaggcaaa
    15841 gggaagttta ggtaaagaac tgatttttta cagcgaatgg acaagaaaac agcttttcaa
    15901 acgtcttcat cactggatgc attttattca gccaatcatt tttttgggcg tagcggcttt
    15961 gattttatta gtttatgccg ctgttttatt acctgtttac ggaagcatag aggaggtttt
    16021 gccatgaaaa agagaaaaat atcttattat agcggtttta cattgatcga aatgctaatc
    16081 gtcttactga tcatcagtgt attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg
    16141 aatcatgtag accaagaaag tcgtgaagcg atcatccagt tggtagacac acaaaaagag
    16201 ctctatgcgc ttcaaaataa tggaagagtt cctactgtgg aggaattgtt gaatgaaggc
    16261 tatatcaaac gagaacatgc agaaatctat cagcggcctt gaaggtaagg ttttagcatc
    16321 aggatatacg ttgtttgaat cactcttact gattttatta cttacaacga tcctttcttt
    16381 tccagttctc gcattttctg catggaagaa tgaattggca gtctatcaat ttttcggcca
    16441 atttgaaaaa cgaatctatt ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg
    16501 cttttattgg aatagtgaag agaatcaaat catttttcat gttccgaatc cagataaaat
    16561 aaattgggaa gtattagata ttcctgatga gattaccttg aaacgccatg cttctattac
    16621 ttttgctgct agaaccggga atgaaagttc actaaaagct tatcaatttt attgggaaga
    16681 aaaagagcgg acgattacgt atcaattcca gatagggagt ggtcgctata caaaaagaat
    16741 tgaataaaac aggatttgtt ttgttggagt cccttacagc gcttatgatc gcttctgtct
    16801 gtgtctactt agtgagcttt ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg
    16861 aggaactgct atttattcga atgatttatg aagatatcaa gacgcagcgg atgtatcaca
    16921 aaagaccaga gcgaagggaa gaaatgatcc catacgatat ccaaattgtc gataataatg
    16981 gagtaatcga aaaagtgttt gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa
    17041 gtaattccag catttacgct gattgaatgt ctaattggac tgatgatatt gagcagtcta
    17101 ttgctcacat tcaatctttt gattcagcaa accatccaag taagtgaatt tttgcagcgt
    17161 aaagatcaaa aagagtggct aatattttta gcacaattag aagaagaact caagagcagt
    17221 cacaatgtct ctgtgaaaaa tcagcaactg cactacacga tcgaggataa acagtatatc
    17281 atcgaaagat atcaggcaat gatacgtaaa agaagaactt ccggcggaca tcaacctatg
    17341 ttaacaagta tcactgagct tcaattattg gaaaaagaac acacaatttc tttttatgta
    17401 cactttgaaa atggagaaga tggatatgcc atatggacaa aaaacgatca gtagaaaatg
    17461 ttataagggc tctattttat tttctgtctt gcttttattt cttttatgta cgtttttatt
    17521 gatggcagca ttagaaagct accgtttgtc aatggatctt aatatacgaa tcaaaaatta
    17581 ttatatagca aaaataatga agcagatggc tttatcggaa cagtctacgc ttgccgaaag
    17641 ttcggaagga gttttttact acacaactgg atctgtaaca tatcagtggg tacaacagtc
    17701 cctattctta gaggtggaag tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa
    17761 tgatacagat actagtacag aatgatggaa tgttcgtgaa aacgccctct tttctgttat
    17821 aacactgttt ttaataaaaa aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat
    17881 tgaaattttg tcaattgttc gatagaatta aacaggtgaa aaaattgtta taatatatct
    17941 agaaatgagg tgcaccatgt taccagaaaa aatggaacaa gggtttaatc aaaacctgga
    18001 agctattcag cttttacaga atgcattggg aacttccttt ttagaagcct atgtcgaaaa
    18061 tgcagaaaat ttaatagatg attatcaagt acgtgtggtg gatggagtgc cgacaaaaga
    18121 gacaacgcaa cggatcacag ctctttatga agagttgaag aaactttctt tcgagccaga
    18181 agaatggcgc agactgtctc aattactttt attgaagggc agccagacag aacatttgca
    18241 ggcgaaccat caactcactc ctgatagtat cggtttttta tttgtttttc ttattgaaca
    18301 actatacaca aataaaaaag caccggttaa gattctggat attgcggcag gaatgggcaa
    18361 cttgttatta acagttcttt taaacctatc gaatgcgggg taccaaacag aaggaattgg
    18421 tgtggatatt gatgatacat tgttggctgt cgcagcttct acaagtgatt tgacacaagc
    18481 aaatgttcaa tactttcatc aagacgggtt gcaggaatta ttaatagatc ctgtcgattt
    18541 tgcaatcagc gatttgccaa tcggttatta tccgaatgac gaaaaagcaa aagagttttt
    18601 aacaagtacc gaagaaggcc atagttatgc acatcattta ttgctagagc aatcaatgaa
    18661 atatgtgaag ccggatggtt ttggtttgtt tttgatgccg agtggatttt tagaaacgga
    18721 tcaaagtgaa gaaatcaaaa aatggtttaa agaagaaggt tatcttcaag gaatgattca
    18781 attgccagat gaattgttcc gtaataaaca atcgcaaaaa agtattttga tcttacagaa
    18841 aaaaggacca caagcacaac aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa
    18901 agaaccagag aaggtgacgg aatttttcaa cgagtttaaa aactggaaat catctgctct
    18961 gtaatcattc aacgagaagg agagtcatat gtctaaaaca attgcaatca atgctgggag
    19021 ttcaagtttg aaatggcaat tatatcaaat gccagaagaa accgtagttg cgaaagggat
    19081 cgtcgaacgt atcggtttac aagattctat ttttaccatc aagtacggcg ataatcaaaa
    19141 attcgaacaa atcatggata tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca
    19201 gttgattgaa ctgcaaattt taagctctta tgatgaaatc accggagtag ggcaccgtgt
    19261 tgttcacgga ggagaaactt atggggattc agtagtgatc gatgagaaag taatggaccg
    19321 cattgctgaa ttagcagaat ttgctccttt gcataatcct gcaaacttaa tggggatcaa
    19381 agcgtttaaa aagattttac ctgacatttt gagtgtggct gtattcgata catctttcca
    19441 ctcaacaatg ccaaagcaaa attatttata tagcttaccg gtgtcttatt atgaagattt
    19501 caaagtaaga aaatatggct tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc
    19561 aaaattattg aacaaaccga tcgaagaact aaaaatcatc acttgccact taggaaatgg
    19621 tgtatcgatc acagcagtcg atggaggtaa atccatggat acttctatgg gattcacacc
    19681 attagctggg gtgacaatgg gtacacgttc aggtgatatc gatccagctg ttctcccata
    19741 tttaatggaa aaattagaga ttgatattga tgaaatgatc aatgttttga acaagaaatc
    19801 tggtctgcta ggcctaacag gattgtctag tgatatgcgt gatcttgaaa agaactatga
    19861 aaaagaacat attcgtttag cttatgacat tttcgtggac cgcatccgaa aatacatcgg
    19921 cggttatgtg acaacgatga atggtgttga tgcaattgtc tttactgctg gtatcggcga
    19981 gaatgacgga catgtacgta gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat
    20041 cgatccagcg ttgaatgaat tgcgtggtga agaagtagat atctctacaa aagaatcaaa
    20101 agtaagagta ttagtgatcc caacagatga ggaactaatg attgcccgtg atgtggagcg
    20161 tctgcgcggc taagccaaag cattcgttag gataaaagat gaaacgcatg caagagggaa
    20221 ttcttgcatg tgtttttttt gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc
    20281 cttctttttt acaggatctt cttagggaat tttgcttgga aaagtggtca aaagggaaaa
    20341 gactggcatt ttatttattg attttcagga aaatcccaat attttatttt cattcatctt
    20401 tataagggac attacatatg taaaaagaaa aaacattttt agatcagcct cgtgggctgt
    20461 cgactttatt cttcgcttta tgtttagcta gaaaaccgat ccgaaacatt cattgatcga
    20521 ataataaaaa cttgattttc cacctgtact ttgcctgaat cgtgaaagca caggtatgaa
    20581 aatcaagttt tttgttgcga aaaaacgtcc tagtcaatca gttggccgag cggctggcgg
    20641 ctgatctgct cctgatcaat aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg
    20701 tcatggctga ctgctaggag cgttccttga tagtcttgca gcatttcttc aatttcttct
    20761 cgagcatagc tgtcgaggtg attggttggt tcatccaaaa tcaggacgtt gattttttct
    20821 aaaaagagtt tcagcaacgc caaacggatt ttttcaccac cggataaatc tttgatccgt
    20881 ttacccacat cgctttgata aaagccactt ttggctaatt gccgtcgagc gacttcttcg
    20941 tttccagtca atgtgatggc gtaagccagc aaacggctat cgggatcggc aaactgcagc
    21001 tgctgcggca aataaccgat ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc
    21061 ttttcctcac cgagtaataa tttgagtaag gttgattttc ctgcaccatt gtcgccgatg
    21121 atggctaaat gatctccacg atagactgca aaactgctgt ctttgaatag tacttgatca
    21181 gcgtactgct ggtgcaagtt ttgaatgatg aaaacttctt tgccagattt tccaccatta
    21241 gacaagcttt gcagccgatt tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc
    21301 aggcggcgtt ccagttcttt ggcttttttg aagaaggact cattgtctcc ttcatgtgcc
    21361 cactgacgaa aacgacgaat catgactttc aaccgctgga cttctttttg ctgcagttcg
    21421 taattttttg tcagctcagc caagcgagct tctttcaaga ctcgaaaacg gctgtagttt
    21481 ccgggatact caatcaatgc accttcttcc agttcccatg tttttgtggt gacttgatcg
    21541 agaaattgcc gatcatgaga aacgaccaga accgattttt ttgtattctg taaatagttt
    21601 tccaaccagc gaatactgtt aagatctaaa tgattggtcg gttcatcgag taaaagtaaa
    21661 tcggcatcgg ctgctaaaat tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt
    21721 tctgttaacc gtgcttcctg acagtgtgga atccctaagc cttgcaagat cgctgcaatc
    21781 cggtcttcta atacgtaacc gcctagttct tcaaagcgat gctgcagctc accgtaacgg
    21841 gccagacact tctctaagtt tgtatctggt tctgtcatca atgcttcgta gcgactcatt
    21901 tgctgtttga ggtcctgtat ctcagcggca gaatggagca agtagtggtt gacggtcgct
    21961 tcggaaactg taagttcttg ttcgaccgtc ccgatcttta agcctttttt gcgactgata
    22021 acaccgctgt cgacaccttc tctgccggtc aagatttgta aaaaggttga tttgccagtt
    22081 ccgttgatat cgactaaggc aattttttca ccagcttcaa tttgacagga taaatcctca
    22141 aataatggat tcccgccgat tgttttttta atagaaattg cttgtatgat catgatctct
    22201 ctccttgaga ccactcaaaa aggccatggt tatccctggc ctgtgttatt taaactcaga
    22261 ttatcatgag aaaaatacct tgagatggcc attgatttta cgtgaattca actgttttgg
    22321 acaaacttgt cccgtcaaca gcaactgtaa aaccaattgc atggctaaat gcgatatggt
    22381 atcgttggta attttcatta cttgttattt tagtcatgcg catcatagta ttttccctcc
    22441 ttcgtgtagt tgtcttttcc agtgtacctg aaagtgtcag aaagttcaac tagcgctgac
    22501 atttacttaa tatttatcga tgtgtaattc tgtcagagag ctttttttat ctgactgagg
    22561 gtaatgtctc gtggaccaca tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca
    22621 tatggtgttt gataaaaaac tttgtctaca tgacttcctt ttgccatttg cttaataccg
    22681 ttgcattaaa cagctcttct ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt
    22741 gtggttgcaa tggcaagcgg tagcctttca tcaattcttg aatgatttac cgaatgtagc
    22801 acttgccttc tcgcggtacc cgaacccgaa ataaaatttt agtttggcga taaaatcggt
    22861 atccgccatc gatacttcat attttttcgt atcagattca tcggaaactt ccacattgac
    22921 gaaccaataa gctttggcat gcttaggtcg ttttaaacca caccagggcg actaatcgcc
    22981 ctggtgttca ctttttttat tttacttgaa ctcattgact gttttccctt tattttaaac
    23041 attataaaga ccgatgagtg cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa
    23101 aaaaacctgc cgatagttgg caggtcgtct acgtgttaag ctttggtgtg gtctttagct
    23161 ggcaattggt tttctaaatc agttcggaca acaagcacat cgcaagctgc gtttctaata
    23221 acatattcag aaacagaacc aataaataaa cgttctacag cattcaatcc tgtggctcct
    23281 aacataatca aatctacttg attatcttct gggatttctc tggcaataat ttgttttggt
    23341 gagccatatt ctacgacaga ggttacatta ttcaagccag ctttcttcgc attgctttca
    23401 taatctgcca atgtttgctt tgccatttca gtagcttgtt ctgcaagcat tccatcaaag
    23461 gatgaaacgg tttggaaaga acgtgtgtca atgacatgtg ctaatagaag ttctccgtta
    23521 tttcggatag ctacattgac cgccttttta aaagctaatt cagcttcatc agaaccatct
    23581 acggcaacca tgattttttt gtattgttgt aacataagca ttcactcctc aagtcaatcg
    23641 tattgtactc ttttattgta gaacacttta gggaaaaaga gaattattta ctgctttggt
    23701 tcagcaattt tttttgcaaa tcggatactg aaggtggcag aaacaataat tacgatcagt
    23761 aaaaagacca aggcaataaa gcgttgattg aaaaaagctg ttaaaatacc cattacgcct
    23821 aaaacagcat atgcagcacc gtaaaattgt aggaatcctt gatttttatc atttttgttg
    23881 atcaatacaa aaaagatatc ctgtttatta aataaataat agctaacgaa taataatact
    23941 aaagtggcga taatcattaa aatcctaatc atgaacaaat cctccaaaat aagcaacaaa
    24001 tagaaaaagc aatttgccaa gagcaaattg ctttattctt taaaagatgg taatctccgt
    24061 taatgccgtt gtttgtcctg tagtattgat cccgcgaatt caagcaggtg ggtcccatgg
    24121 tcgtagcttc gaactaccaa atgataaggc atgttaccag cagtgacaca agacgggtcc
    24181 caagatatca ataaaaatgt tcggtcaaca cacaaagagg acaattcgaa catcacagat
    24241 ttcccacctc tatagtagca tatgaattta aaaagagcaa tgagagagct cgcagaaaca
    24301 gaaccgtttt cttgtctagt tttttggagg atgctttttc cactctttta tacggtaata
    24361 ctgttgaccc agagcctgtt catatttccc tgtatctttt ggctgataat actgagcatt
    24421 cttcagttta tctggtaaat attgctgatt gacccaagcc tgatcaaaat gatgaggata
    24481 ctgataaccc acgccacggt tgagtgattt cgcacctttg taatggctat ctctcaaatg
    24541 atcaggaaca tctccagctt tgccctctcg aatatcagca agtgctgcat ctaatgccat
    24601 gtaggcagaa ttggattttg gcgacaagca aagatcgacg acgacgtctg ctagtgggat
    24661 acgtgcctca ggcaatccca atttttcagc agctaataca gcgttcactg tacgagccgc
    24721 agcagcagga ttacctaaac ctatatcttc ataaccgata accatcagac gccggcaaat
    24781 acttgccaga tcacctgcct caactaaacg ggcgagataa tgaagtgcag catccacatc
    24841 acttccacga atcgattttt gaaaagccga aataacgtca taatgagcat ctccattttt
    24901 gtcatgggta agagcttttc tttggacaca ttcttcaatg atcgacagtg taagatgaat
    24961 ccttccttct ttatctgaag gagtcgatag tgtagccagt tccagcccat tcaatgcact
    25021 ccgcaaatcg ccgttcgttg cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat
    25081 tgcttgttgt ccaaggccgc gttctttatc ccttaaagca tgttcgactg ccaactggat
    25141 gtcttgttct gttaatggtt tgacttcaaa gatctgtgta cggctgcgga tcgctggatt
    25201 gatggtaata taaggattct cagtagtcgc tccgatcaag atgattcggc cactttctaa
    25261 atgaggaagc agaaaatcct gttttgtttt atctaatcgg tgtacttcat ccaataagag
    25321 gatcactgta ccactcattt ttgcttcttc cgctacaact tgtagatcct tttttgaatc
    25381 tgtagcggcg tttagcatgc gaaatgcata attagtagag ccggcgatcg cacttgcgat
    25441 actcgttttt ccagtaccag gaggtccata tagaatcatg gaagagagca tgcgagcatc
    25501 gaccatgcgg cgtatgattt ttccttcccc gactagatgc tgctgaccaa cgacttcatc
    25561 gatcgttcga ggtctcatgc gataagccaa tggctgttgc ataggatttt cctccttctt
    25621 gtttttacgt cccttaatta tatcatattt agcaagatgc ctagaaagaa cagccttctg
    25681 ttgttttgtt ttcatagctt atccaactaa tattgtgtat gataaagtag gtgagaaaaa
    25741 tgacggataa acaaaagata gatgaatttt tttggaataa aacgacgcca gaagtggcac
    25801 gggatttatt ggggatgtat ttggagcatg acacaccaga aggaagatta gctggatata
    25861 ttgtagacgc ggaagcatac ctaggtccag aagacgaggc cgcccatagc tatggtttaa
    25921 gaagaacacc gcgtgtgcgg gcaatgtatg aaaaaccagg tacgatctac ctttacacga
    25981 tgcatactca tcggattttg aacatcatta ctcagccaga agggattcct caaggagtaa
    26041 tgatacgtgc aatcgaaccc gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa
    26101 aaactggtcc agatatcagc aacggtccgg gaaaattagt ggaagcatta gcaattcctc
    26161 aagaacttta tggtcagtct atagcagata gttcgttacg cttagttttt gagaaaaaaa
    26221 agacaccaaa aaagatcaat gctttgccta gaatcggtat cccaaacaaa ggtgtatgga
    26281 cagaaaagcc gttgcgtttt gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa
    26341 accaaatcga gaaagattgg ggttggagga aagaaaatga aaaagaagga tctattaaca
    26401 tatttagatg aaataattga aaaacaagtg acagactatg atgtagcttt agattgggat
    26461 acaaaaaatc atacgatcga gatcgttgtt cgcttgtttg cagaaaataa agcacaattt
    26521 gagattgatg atgcagaagg gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc
    26581 gtacttcttt ttaatcctca gaaatctgtt tttgatgaac gcaattatct agcagtaatc
    26641 ccttatgaag gaaaaaaagg gttggcaaaa tcagtagctg attctttagt ggaatatcta
    26701 aatgaagtat tagctcaagg gcaaagcgat ttgcttgatt ttttggatga agacgatgac
    26761 gaagctgtat tcgagcttca ctgggataat caacaactag cagaattagt agaagtaaaa
    26821 cagcaaaatg aagcagatac ttatttaccg tacccaagtt attaaggagt gacagatatg
    26881 aaatggaatg aagtaaaagt agaaactgaa agtgaagcag tcgaagcagt tgctaatatt
    26941 ttgatggagg caggggcgag tggtgtcgcg attgaagatt ctttagatgt tgaacatttt
    27001 cagtcggatc aatatggtga gattttagat aaaaatgatt ttacatcgct gaaagaaggc
    27061 gcactagtca tggcttattt tccggaaacg actttcttac cggaaatctt gccttttatc
    27121 aaagatcgta tcacacaatt acctgagttt ggtctagcta ttggaaaaaa taaagtaaca
    27181 gttagtgaag tagaggaaag caactgggca acagcctgga agaaatacta tcatccggtg
    27241 cgtattacgc gaatgctgac ggttgcgcca agctgggaaa actaccagac agaagatcct
    27301 ctagaaaaaa ttatcacact agatcctggt atggcatttg gcacaggcac tcatcccacg
    27361 acttccttga cattgacagc actagaaaca gtcttgcgtg gtggtgaaac attgctagat
    27421 gtgggaactg ggtcgggcgt gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc
    27481 tatgcctttg atttagacga agtagccgtt cgttcggcga aagaaaatat ggatatgaat
    27541 gaaatagcca aagatgtcca tgtatcagct aacgatcttt tgacaggaat cgagattgaa
    27601 agtgatgtaa tcgttgcgaa cattttagca gatatcatct tattgatgat cccagatgct
    27661 tggcgtttac tgaagcaaac aggcacgctg atcgtttcag gaatcattga agagaagaag
    27721 caaatggttc tggatgccat gttagaacaa gggtttatcg tggatcaaat tttccagcaa
    27781 aaagactggt atgcaattat tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt
    27841 ttaaaagaat catatgaatc aaaagactat tttgttttag aaggggaaaa ctatcaccac
    27901 attgtacggg ttatgaggat ggaacccgag gatgaagttt ttttggcatt caatgatcaa
    27961 atagccattg ttgcagaaat cacggagatt actgaagagg ctgtttatct gaaagaaatt
    28021 aaaaaagaag aaaatcaaaa ggaactgcca agttcgatta ccatagcgag tggctatcct
    28081 aaaggggaaa agctggattg gatcgttcag aaatccactg aattaggagc gcaagctttt
    28141 cttggtttcc cggcatcagc ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga
    28201 caacaacggc ttgaaaagat tgctcaagag gcagcggaac aatcccatcg gcagatcgtg
    28261 ccatcagtag agttattgga aacgaaacaa gatttatttg agcggctaga tgattatgac
    28321 gtggtcttgg ttgcttatga ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa
    28381 attcttagtg aaacaaaacc agatagtcga atcttagctg tctttggacc agaaggtgga
    28441 ttatcccgcg atgaagtcga ttattttgta gaacacaaag ggatactttg tggattagga
    28501 ccaagaatcc ttcgtacaga aacagcacct ctttatttat taagtgctgc tagctatcaa
    28561 tgggaattaa tgaatccttc tttcagctaa cgttcttcat taataaaaga gtatcgacag
    28621 aaagaattga aaaaatagtc aacaatttgt ataatagaaa caattattta tacaatggat
    28681 acgaaaaatc gtttgaaaaa gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac
    28741 tgcacggatt ttgggacaat tatcaaacag atatttgtcc cgattcccgt tttaccgaaa
    28801 aaagaaaggg ggatcgattc ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat
    28861 aaagctcgtt agtcaataca tgggtcccga acatgtagca tttgttgaga aagcatgcga
    28921 atacgcgact gctgcacatg acggacagtt tagaaaatca ggcgaaccct acattattca
    28981 tcctatccaa gtcgcaggta tattagcaga tttaaaaatg gatccccata cagtggctac
    29041 aggcttctta catgatgttg ttgaagatac agaaatcact ttagaagatc tgagagaaga
    29101 atttggcgat gacgttgcta tgttagtgga cggcgtaacc aaattaggga aaataaaata
    29161 taaatcccac gaagagcagc tagcagaaaa ccaccgaaag atgctgctag caatggctca
    29221 agatttacga gttatcatgg tcaaattagc tgatagacta cataacatgc gtacgttgaa
    29281 gcacctgcga gaagataagc aaagaaggat cgctcaggaa actttagaaa tctatgcgcc
    29341 tcttgcacat cgtctaggga tcagccggat caaatgggaa ttagaagata cagcacttcg
    29401 ttatctaaat ccaaaacagt attaccgtat cgtccacttg atgcagacga agagagaaga
    29461 acgtgaaaaa tacgtaagtg gtactgttga agatattcga atagcgacag aagagctggg
    29521 gatttttgca gaaatctatg gacggccaaa acacatttat tcaatctatc gtaaaatgaa
    29581 agatcagaaa aagcagttca acgaaattta tgacttgcta gctattcgag tgatcgtaga
    29641 ttcaatcaag gattgttatg ctgtattagg agcaatccac acaaaatgga aaccaatgcc
    29701 cggcagattt aaggattata ttgccatgcc aaaagctaat atgtaccaat ctttgcatac
    29761 taccgtgatc ggtccggcag gaaatccggt agaaattcaa atccgaacac aggaaatgca
    29821 tgaaatcgct gaattcgggg ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa
    29881 agtagaacca gatggtatga cgaaacaatt aagctggttc catgagatac tcgaacttca
    29941 agacgaaagc tatgatgctt ctgaatttat ggaaggcgta aaaggagata tctttagtga
    30001 taaagtctac gtcttcacac caaaaggaga cgttactgag ttaccaaaag gatccggacc
    30061 attggacttt gcatacagta tccatacaga tatcggtaac aaaaccactg gtgcaaaagt
    30121 aaatggcaaa atggtgcagc ttgattacaa attgaaaaac ggagatatca ttgagatcat
    30181 gacttctcca aattcatttg gcccaagtcg cgactggttg aaattagttg ctactagcaa
    30241 agcaagaaat aagatcaaac gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa
    30301 aggccacgaa tccgtggtca aatgtattac agatctagga tttacgccta aagatatttt
    30361 gacgaagaac aaactgcaag aagcactcga tcgttttaat tatcaaacag aagatgatct
    30421 ctatgcagct gtagggtatg gagaagttag ccccttgacg atggccaatc gtctgactga
    30481 aaaagaacgt aaagaacaaa aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa
    30541 tcagccgaaa aaagaacctg acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca
    30601 aggggtagaa aacttactaa ttcgtatcag tcgctgctgt aatccgattc ctggtgatga
    30661 tatcgttggt tatatcacta aaggcagagg gatatccatt catcgtcgag attgtccgaa
    30721 tgttcagcct gacaaaccaa atgtagcaga acgtttgatt gaagtcgaat gggaagatac
    30781 atcgaataca cgaaaagagt atgatgcaga tttggaaatt tacggctata atcgttcagg
    30841 cttattgaat gatgtacttc aaacagtcaa tgcgctaacg aaaaatctca acagcgttga
    30901 agcacggacg aataaagata aaatggcgac gatccatttg acggttggta tccagaattt
    30961 atcccaccta aagagtatcg tggataaaat caaagcagta cctgatgtct acagtgtacg
    31021 ccggacgaat ggataggagg acagatatga gagcagttgt tcaacgggta agtaaagctg
    31081 ctgtgtcagt cgatcagcaa gaaattggaa aaattggcta cggcctactt gttttattag
    31141 gcattcatga aaccgatacg caaaaagatg tggactatct ggtaaaaaaa attgcacaaa
    31201 tgcgtatttt tgaagatgaa caaggaaaaa tgaatattag tgtagaagat gtgggaggac
    31261 aaattttaag tatctctcaa ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa
    31321 gctttgtgga agccgcacga ccagaaacag ctattccttt atacgaatca ttcaatgaag
    31381 gaatcagaag tcgcggcatc acagtcgcta ccggacaatt cggagcagat atgtcggttt
    31441 ctctcgtcaa tgatggacct gtaacgatca ttattgattc taaaaacaaa taaagtaatc
    31501 tggttttcac tagtaaaaac tatgaaacac gtcgcttaag ttagactgat aaactctaac
    31561 ttaagtggct tttttgtctg caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta
    31621 agcttaaatt atacaagtta ttttttatag gaggttgctg aagatgaaag aagtaccagt
    31681 agtagatatc aaaatcacag atttaaaaaa attgtatcaa aaaggataca acatgttgga
    31741 ggagttacgc catgaagcgg acgctcccgt cgtcaaagca aagattttca ataaagaagc
    31801 tatcacgatc tacggatcat cagcagcaaa agtattttat gatccaagga atttcaaacg
    31861 aaaaggtgct atgccaaaat tagtattgaa gacacttttt ggtcaaggtg gagtacagac
    31921 tttggatgga gctgctcatc accatcggaa aaatattttt atggatctaa tgacaccgga
    31981 aagaatggaa gattatcatc gtatcttaga taagaatctg acacaagcac ttgaagcaca
    32041 gcatggtcaa tttgaactat ttgatttaag taagatggtt ttctttacat ctatttgcga
    32101 gtgggcaggt atcaatttat cagcgatttc taaagatgag gtcgaaaaat tagcagaata
    32161 tcaaatctct atgatcagcg gaacgtttac ttctccgatt gatcacataa aaggtgtcga
    32221 gaatcgcaaa aaatcagaaa aatgggcgca aggcttgatt gaagaagctc gacaaaatcc
    32281 tgtggctgga aaagaaaatg tggcattata tgcttttgcc aatgccacag atttagatgg
    32341 tcagctttta cctctggaag tggcagctgt tgaattattg aacatcatcc gtccaacggt
    32401 tgctctaact gtgtgggcgg cattgatggg acatgcactt ttcagtagac cagatttgta
    32461 tcagcaatta aaaaatgact tttcgacgtt acaagatccg tttattcaag aaatgagacg
    32521 ctattatcct ttcttcccaa tgctacctgc gatttcgctg aaagaagtag aagtagatgg
    32581 ttatcgtata ccagaaggca gttgggttat tttagattta tatggaacag accatgatga
    32641 acgaacagtt gaagcgccag attcctttat gatcaaacgg tatgtaggga aagcaaaaga
    32701 tatttcctat aaagaagaat atgagatgat tgcacaaggc gggggaaatt ttcgacaaat
    32761 gcatcgctgt gcaggagaat ggatcactct tcatagtcta cgtgtgtttt ccgatcagct
    32821 agtaaataag tttgagttct ctgtacctga acaagattgg acgattccat tcaatcagtt
    32881 tccgacttat ccgaatagcc gagcgctact ttacaagaat tagattagca taaacttatc
    32941 gaaaaaaagg aagatcaaac agtgctgatc ttcctttttt catattacta taaattgata
    33001 aggtcaattg atcaaaatcc acgaaatgct tcatcaatgg tttgataatc ttcattggat
    33061 aaatgcaaat ctaaagcttg gacattgtca gatacttgct ctggacgctt agctccaggt
    33121 ataactacag acactcgtgg attcttcatg taccaagcta aaacaagttg cgcaggagtg
    33181 gcttggtatc ttttagcaat ggggcgaagt acatcgacag cggttaaaat ttctctgaaa
    33241 cgttctcctt ggaaatctgg atttcttttt cgaggatctc cttcaccaaa ttgtttttcc
    33301 tcaccaagtt catattttcc tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa
    33361 ctgattttgt ttttttcaag ataagggaat aattcttttt cagcctgccg atggatcaaa
    33421 ctgtatttat cttctactac atcgacatat ccatctgcat tcgcttcttt caattgttct
    33481 aacgtgaagt ttgacacacc gactgctcgg atttttcctg cttcttttaa ttcgtgcaat
    33541 gtagcaactg attcattttt tggtgtgctt tcatccggaa agtggatata aaaaatgtcg
    33601 atgtaatcag tttgcagacg ttttagtgca tcttccaccg attgtttgag aaactcagga
    33661 gaattatcaa atgttcgccc tttatttggt acatgagcag cttttgtagc gatgacgaca
    33721 cgtgagcgat catattcttt ttccttcagt acctcgccaa tcagttcttc ggaacggccg
    33781 tttccgtaag cataagcggt atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact
    33841 acttgttttc ctgtctcatc ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt
    33901 gctcctaatc caagctgttc tgcgtagact tgtgaatgtc cgattcttat ttctgccata
    33961 gctgattcct cctttttgtc tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt
    34021 tttgtgaggg gaaatagtca gatatctctt gactttgaga ctggttttta gtaggttata
    34081 agtaatatcg aatgtcaatg aaagagcgag taagtttttg aaactttaca agagaggatc
    34141 atcgaggctg aaaatgatct aaagtttcat tagcctaaag acactctgga gatggacgag
    34201 tgaaaagccg tcccgcgtac gagcgttatt cgtaagagga aagagaaatc tttcaaaatt
    34261 aggtggtacc gtgtatgaat ttcattcacc cttgttcatg atgaacaagg gtgatttttt
    34321 tatttacata gctaagaaag ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa
    34381 atgatatttt gccaggcgaa tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat
    34441 tattcagaga ttatcagtat aatgaaatgc gtacaccgat ttttgaacac tatgaagtga
    34501 tatcccgaag tgtaggggat acaacggata tcgtttcaaa agagatgtat gatttttatg
    34561 acaaagggga acgccatgtg acgcttcgac cagaaggtac agcaccaatc gttcgttctt
    34621 ttgttgagca taaattgttc ggcccagagt atgcgaaacc gttcaaaact ttttatatgg
    34681 gaccaatgtt tcgctacgaa cgccctcaaa aaggacggtt acgtcaattc catcaaatcg
    34741 gagtagaagc atttggatca gaaaaccctg caactgatgt ggagagcatg gtgatggcac
    34801 ttgatttctt caaacagttg gggatcacgc aaatccggct agtcatcaat tcattaggtg
    34861 ataaagaaac gcgtcaagcc tatcgtcaag cattgatcga ctatctttta ccttttgaat
    34921 cagagctgag tgaagactct aaacgccgcc ttcatgaaaa tccattacgc gtattggaca
    34981 gtaaagacaa acgagatcaa aaatttgtgg cagaagctcc atctatctta gattatctaa
    35041 gcgagcctgc tcaaaaacat tttgataccg ttgtttccat gctggatgca ttggctgttc
    35101 cttatgaaat cgacagtaat atggttcgcg gactagatta ttacacacat acgatttttg
    35161 aaatcatgag tgacgcacca aaaatggggg cacaagcaac gatttgcgca ggcggacgtt
    35221 atgacaatct agtggaagaa ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg
    35281 gtatcgagcg attactcttg actatggagg cagaggaagt agttatccct gcatttaatg
    35341 agttggatgc ttacgtggtc gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg
    35401 tccaagcgat tcgaaacttt ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa
    35461 aagcgcaatt taagacagca gataaagcga atgccaaatt agtattggtg atcggtgaag
    35521 atgagcttgc tagtcaaacg attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt
    35581 ttccattaga agagatatat gaacattttg atgaagtcta tgatgaaatg actttattcg
    35641 agtaggagga aaaaaataaa tggcacaaag aacgatctat tgcggattag tttccgaaca
    35701 acaattagaa caaacagtta cattaaaagg atgggtacaa aaaagacgag accttggggg
    35761 agtaatcttt atcgacctac gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga
    35821 aatcaacaaa gaagcatggg aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt
    35881 gactggggaa gttagaagac gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga
    35941 attcgaagtg attgcaagtg aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca
    36001 aattgaagat gatcaagcaa tcagtgacga aatacgtttg aaatatcgtt atttggatct
    36061 gcgtcgtcca aaaatgacta aaaacatcaa attaagaaat gcgacaacaa aagcgattcg
    36121 tcattattta gatgataatg actttctcga tatcgaaaca ccttacctag ggaaatctac
    36181 accagaaggc gcaagagact acctagttcc ttcacgtgtc catgctggac atttctatgc
    36241 gttgccgcaa tctcctcaat tgtttaaaca attgctgatg aatgctggat tcgaccgtta
    36301 ttatcaaatc gttcgttgtt tccgcgatga agatttacgt ggagaccgtc agcctgaatt
    36361 tacgcaagta gatattgaaa caacgttctt aacagcagaa gaaatccaaa cttacacaga
    36421 gggattgatt gcgaaggtaa tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc
    36481 tcgtatgacc tatgatgaag caatggctcg ctacggcagc gacaaaccgg atacacgttt
    36541 tgatatggaa ttgatcgatc taagcgatac agtcaaagaa gtagaattca aagtcttcca
    36601 aatggctctt gaaaacggcg gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg
    36661 ctactcacga aaagacatgg atcagttagg tcaatatgta ggacaattcg gagctaaggg
    36721 gcttgcatgg ctgaaagtag aagaagacgg attgaaaggt ccaattgcta aattcatggg
    36781 agaagcaaca gaagcaatca ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt
    36841 tggcgcagat aaaagtgaaa tcgtcgctgc tgcattaggt gctatccgga cacgtttagg
    36901 aaaagaatta ggactaattg atgaatcgaa attcaatttc ttatgggtaa ctgattggcc
    36961 acaatttgaa ttttcagaag aagaaggtcg atatgtctct gctcaccatc cattcacgat
    37021 gccaaaagaa gaagatatcc cgttattggc tgaagaccct gcaaaagtct atgcagaagc
    37081 ttatgatatc gtcttgaacg gttatgaatt aggcggaggt tctatccgta ttcatactcg
    37141 tgagcttcaa gaaaaagtac tagaaactct aggctttaca aaagaacaaa tggaagaaca
    37201 atttggtttc ttgttaacag ctttagatta cggattccct ccacatggcg gtattgcatt
    37261 aggattggac cgttttgcaa tgttattagc cggagaagat aatattcgtg aagtcattgc
    37321 ctttcctaaa aacggaaaag cagcagatgt gatgagtgat gcaccaagta tggtttcgcc
    37381 tttacaattg ttcgaattga atattgatgt aacgaatata gaagaatagc cgataaaaag
    37441 gtaaaaagga tttgtcgaat acgcttcttt tgacaacctt gatttaattg aaaaacactt
    37501 tataatgaag ccataacata taaagcgggg cttttccatg aaaaaaacat tcgaaaattg
    37561 ggcttatcat gattacacga ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc
    37621 agtcgcattg aatttctttt atcagccagg acatatttat tcaagtggta ttactggtct
    37681 agctcagatt ttgacgactt tgtcaaaatc actgatagga ttcgaagtac cggtatcaac
    37741 aactttgtat ttactgaata tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa
    37801 gtttacaatt ttcactttta tcacagtcac tttgacctcc ctttgtatcc atattttacc
    37861 ggaaacagtc ctatctaccg atccaatcat ctgtgcgttg tttggggggg ctgtgatggg
    37921 actaggtatt gggctagcgt tgagaaacgg cctatcatcg ggcggtctag atatcgtgag
    37981 tatcacgatt cgtaaaaaaa caggaaagtc gattggttct atttcgatct acttcaatgc
    38041 attgatcgtt tttgttgccg gaattctatt tggctggcaa tatatgtttt acagtacgtt
    38101 gtctattttc gtcagtggaa aagtaactga tgtcgtttat actaaacaaa agaagatgca
    38161 ggtaatgatc gtaacgaaac atccagaaaa agtagtaaat gaaatccaaa actgtatgcg
    38221 tagagggatc acgatcatca atgaagcaga aggtgcttat aagcatgata agcagacagt
    38281 attgatgacg atcgttacac gatacgaact gcctctactt agagaagcaa tgaaagcttc
    38341 tgatccacaa gcttttgtaa gtattgcaga aaacgtccag atacttggtc gtttctatga
    38401 ggatggcatt tagatcgagt ttgacaaaaa ttcatgaaat actgctgtag acagacgttc
    38461 gtttgtctgc agtttttttc taccatagta gaatcagcaa agaaaaactg cttttttatt
    38521 tgaaattttc ttaaagttga aaaaaagcat gtttattttt ttaaatttat gggatgatag
    38581 aaaaaaatag tagagaaagg acacgattct acttgctatt ttcacttaat acccgtatat
    38641 tacgaatgaa aaaagtttat gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa
    38701 aaaacagccg ctacatcact gggtttgatg ggctccgttc attagctgtg gtgggtgtta
    38761 tcttctatca ccttctgcca actagtatga aaggcggcta tttgggcgtt ccgatttttt
    38821 tcgtcgtatc tggttatttg atcacggatc tgctgcggca ggaatgggaa caaaatggaa
    38881 aaatcaatat ttggcaattt tatgtcagaa gaatgaagag actctatccg gcacttgttt
    38941 ttatgcttgt gacagcttcg gcttatatca ctttgtttca gcgagggctg ttaaacaatc
    39001 ttcgaggagt tgtggtcagc agcttactgt atgtcaacaa ctggtggcaa atcaataacg
    39061 gtttatctta tttcgatcga tttgctaatg agtcaccgtt tacgcatatc tggtctttag
    39121 cagtagaagg tcaaaactat ttggtttggc cgatcttgtt tgtattgttg atggtatttg
    39181 ttcgtaaaag aaaatggatc ttttatactg tgttaggtac atccttcctc tccgccatct
    39241 tgatgatgat cttgtataca ccaggaggcg atccaacccg tgtttattac ggtaccgaca
    39301 ctcgtctgtt ttctatatgg ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt
    39361 taaagaaaaa catcccgaaa caagcgaaaa atattctgaa tatcgctgga ggaatctcgc
    39421 ttttggcact gattctttgc ttcttctttt tagatgatca ttacaatttc atctattacg
    39481 gaggaatgct cctagtgagt ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg
    39541 gagcaagttt gaatcgatgg ctgacaaatc cagtatttac ttggattggc aaacgaagct
    39601 atggcattta tctgtatcag tttccagtta tgatttttta tgaagcaaag gtgactgata
    39661 ttgccgatca tgttttgttg catactttag tggaaatcac attgattcta atcatcagtg
    39721 aactttctta tcgatatgtg gagcgtccgc tagcacgttt tgattatggt cgtacattcc
    39781 aagtgatgaa aggatggttc agcaaaccga tcatcagtaa gagcaaacca tgggttgttc
    39841 caggcacatt acttgtagca attgcactcg ttggtttcgt tacggcaccg aaaaacgctg
    39901 taacagccga tcaaaaacaa ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag
    39961 aaaccagaaa caattcagaa aacaccacta gttcggttga ccaagcgatc cttgataagt
    40021 acgagttgtc caaggcacaa gggaataagg cacaaaaatt agaactaacg gcatttggtg
    40081 attcagttat gttggatgct gcaacggatc tacaagaact ttatccaaaa gtagtggtag
    40141 atggagatgt cggacgacaa ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac
    40201 aagatttgtt gaaagacact gttctgattg gactaggaac gaatggctca ttctcggaag
    40261 ctcagtttga tagtgtgatg gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc
    40321 gagtaccgac gaagcgctgg caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa
    40381 aatacgacaa tatgacgctg attgattggt acagctacag taatgaccat agtgactggt
    40441 tttatgatga tcaagtccat ccaaatcctg aagggatgac acattacatt cacttagtca
    40501 gcgaagcctt gctaggagga agttcaagta cgaacagcag tgagacaaac gtgacagaat
    40561 gatagctaca aaaaacttca ggcaaaaaca tttgtttttg cctgaagttt ttttatattt
    40621 accgtgtttt taacattcat atagattcag gaagcaaaac ggttgagtgt gtctgtaaat
    40681 aacgttcttt ttgttttcgg gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc
    40741 ttcttgaaga aaatacttct gcatggatca tcttaacagg tcttcttttt tgaagacgag
    40801 tatatttagc accagtacca ttgttatgtt cctctaatcg tctagaaaga tcggttgtat
    40861 atccaccata aaatgtctga tctttacaga ataatacgta aaaataatgt tcatttgcca
    40921 tagagcatct cacgaatctc tgcagtgtat tctccatcca tattgtaagt gatcaatggt
    40981 ggagcgactt tgaatccgtc taatttgcct tctttgattc cttcaactaa caaaatattt
    41041 gcctcttttc caggttttgg gtaaacaaat tggatccttt taggtgcaat tcgatagtct
    41101 tccatgacct gaatgatttc taagaaccga tcaggacgat ggaccatagt aaagcgtcca
    41161 tttgttttta gtagcttact tgaaatatcg atcacttctt ctaacgtcgt atgaatctca
    41221 tgacgggcga ttgccaggtg tttattaggg ttcgttttat ttgttggcag ccctttgaaa
    41281 taaggtggat tacagacgac taagtcacaa gaattatgtc gaacgacaga taagctgtct
    41341 ttcagatcga tggtgtggac agtcatttgt tcatttaaat ggttaagcgc gatactacgt
    41401 cttgccatgt ctgctagacg aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt
    41461 ttacttaaaa aaagtccaac tgcgccattt cctgcacaaa ggtcaacaat cttccctttt
    41521 ttaggtacac gggaaaagtg agcaagcagc acagcatcta aagaaaaaga aaacactttg
    41581 gaactttgga taatcttgat atcttctgcg tataattggt cgattcgttc gtcttcgtga
    41641 agcatggatt ccctccaaaa tgaaaaaaat agtcgtctgt attatactat ttttattaaa
    41701 aagaaccaag gtggcttgtc aaacctctat tttttatgca tactatgacc ataagaaggg
    41761 aaaggaagag actatgtttt atcgatttat gcgcggggta gttcgagtac tattagcctt
    41821 agttaatgga aatgcccgct atgagaataa agaagtttta ccgcaagatg agaattacat
    41881 actagttgct ccccatcgta cttggtggga cccattatat ctggcggtag ctgcagcacc
    41941 caaggaattc agctttatgg caaaagaaga actgttcaaa aatccagttc tgcgatttat
    42001 attaactcga tccaatgctt ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa
    42061 aacacctgta aagattttga aatcgacaaa tttgagtttg atcatgtttc ccagtgggac
    42121 aagacatgct acagaactta aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa
    42181 aatcgtgcca gctgtatatc aaggtccgtt gacacttggg gatctgttca agcgtcagag
    42241 agtgacagtg cgtttcggcg aaccaatcga cttatcagat atcaagaaaa cggataaaga
    42301 aggtatggcg gagatcgaac gacgaacaca agcagctttt gacaaattgg acaaagaagt
    42361 gaaccctgat ttcaaatatg aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg
    42421 gtcttttgaa tctactgtcc tgttcagttc agactttttc aatgaaaacg agggagagac
    42481 gtcaagtctg ccctcttttt gcgttccaat atgtaaaata gaagacaggt gtcatgtaat
    42541 ggatcaaagt gttataataa ttagaaggac attttttgag gtgattgaat gcgtttttta
    42601 catacagccg attggcatat cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag
    42661 cgtcatgtac tatcttctat actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg
    42721 attgccggag acttatatga taggagtgtc ccagctgttg atgccgtaga attatacaat
    42781 gaattgatgg tcgattggaa tttaaaggaa aagatcccga tttttgctat atcaggaaat
    42841 cacgatagca gtacgagact ttcagcgggt acgccatggt tcagttattc gaactatttt
    42901 ttgcgaacac agctagcaga agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc
    42961 tttcttcctt actttgaacc aattgcagcg agattatatt ttgaagatga caatatccga
    43021 acgatcaagc aagcggtagc caaggtgata gataaaatgc aggagctatt tgattcagaa
    43081 aaaaaacaag tattgattag ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa
    43141 acgaaaatcg aagtaggcgg gttggattcg atccctggtg aactacttac accgtttgat
    43201 tatgtagcac taggacattt gcataatcaa gctgctttga gacaggataa tgctcgctat
    43261 agcggttccc cattgaaatt ctctttatct gagatcaacc aaaaaaaagg cgtatggatc
    43321 gttgacctga caacaggcct ggagttgaat tttagggaga tcgaacccct gcatgatatt
    43381 gaagaaataa cagcgagttt caaagaattg ctggatcctg ccttttatca gtccgtcaat
    43441 cgggagaatt acatctatat ctatctcact gatagaacga tcatcccgaa tatgatgaac
    43501 cagcttcggc aaatatatcc tcgtattttg gggattgaac gggcaaacgg tcgagcagaa
    43561 caaacaaagc gcaaacgaaa agtactagaa atgaaagatc ctcaaaaatt agcagaagca
    43621 ttttttagtg aaatgacgga agagcagcta actgcaaacc aagaaaaatg gctcggagaa
    43681 acgttgcaag aaatcatgga aatggaatga tcaagaatga aaccgaaaaa attacaactt
    43741 aaaaattttg gtccttttat caatgagacc gtagattttt cacgtttgac agaagcaccg
    43801 ctttttttga tcagcgggaa gacaggagct ggcaaaacga cgatttttga tgggatcacc
    43861 ttcgcacttt ttggtgaaac ttccggaaga cttcgctctg gaaaagagat gcgttctttg
    43921 tttgctacac cagacgaaga aacaagcgtg actttttctt ttgaacatca gcagatgagc
    43981 tatgaaatcg aacgaaaacc agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa
    44041 aaacaggcag ccaaagtaac gttgaccatt tttgatggga aaggaaaaga aataaaacaa
    44101 atcacaaaac gtacagaagc agatcagttg atcagagaac tgatgaatct cgatgcgaaa
    44161 cagttttctc aaatcgtttt actaccccaa ggagaattcc gtaatttttt ggtttcatca
    44221 agcagtgata aagagactgt tttacgtcat ttattcggta ctcaattttt ccaacagttc
    44281 aatgagcgat taaaagaaaa agcaaaacaa cagcaacaaa aattggatca tttagagcaa
    44341 gagctgcatc ttctgcaaaa aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg
    44401 tttgaagccg ttttagccca atggacaaac catcaggcaa tactcgaaaa acaaatcacg
    44461 gaagaaaaag aagctctaac ggaacttcag gaaaaacaaa agcaactaga aaattcctac
    44521 tatcgttttg aaagcttgaa aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa
    44581 aaggaattag ctgctcaaaa agacgaaata gaggaaaaga aacgatggat tgggtattat
    44641 gaatttacgc aacgattagt cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa
    44701 atagaactgt cacaaaaaag tagtgagcaa acaaagaaac tagcgatgac tacgcagaat
    44761 tatgaagagt ggcaaaaaaa tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa
    44821 gctgctcaaa aactgcaaaa caaacagatg atggtgccaa ttgtcgaatc cttacgtagt
    44881 aaaaaacaag acgcagaaca attaacaatt tcattggata aagaagaatt gaatgccgag
    44941 aagctgtatc aaaatctcgc tatctatcag gaaagaactc gaattcttca agaaaattta
    45001 ctggatcagg aaaaactgca agaagatcgt cttgcttttg gacagttaaa atatctagat
    45061 gaaaagcagc aagaaataga aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata
    45121 agagaagaac tcaaaaaagc aactgaagaa ataacaaaaa atagtcagct attgaaagaa
    45181 cagacagaac aagcagaaca gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt
    45241 cagctttttt tgcgtccagg ggagccatgt cttgtttgtg gatcaacgga acaccccata
    45301 caacaattaa aacacgaaca gccaaataag gaagatatct taaaagtaga acaacaactg
    45361 gcagaagctg agtctgctgt agaaagaact cagcaaatca ttgccaaaag tgaagcacat
    45421 gaaaataatc ttcaacaaca attatcagat ttagaacaaa aaatcatggc aacgaatata
    45481 caattggctg aacaagaaga acacttgcaa caggctttga aaaaggcatt tcctgagata
    45541 acagaacaaa aaacaaaaac aactcttgta gtaatcgaac aatctttaga tagacgaaaa
    45601 agcaagatgg ccaatgaaag aatagagctg gaagaaataa gacaacaagc agaaaagctc
    45661 gaacaacaat tagctgtcta caaacagcag cagacagaaa aaaataatca gctccagcaa
    45721 ctgcaaggag aaatcaaaag tttagaacaa caattgcaag atacggatag taactgttta
    45781 gaagaggaga tacgacgact tgaaaaacag ttagaagaag actcgaatca actagctgtt
    45841 gagcagaaag caggagtgca attgttgcaa gatcttcata cgcttcggac acagcttgat
    45901 cttcaacatg aacaaataga aaaaatagat gaaaaaatag aaaaggaaca agaaagaata
    45961 gctaaaaagt tagaaagcca gtcgtatttt tcaacaattg aagaaatctg tgcatttgca
    46021 caacagcaag cacaatataa agaaaaccaa cgtacagtta cttcttatca tgaagagcaa
    46081 ttagtgacag ctgaccggct gcgtcaatta gaaaatatcc agctaactga gacattccca
    46141 gatacagaag agatcagcca acagttagaa gaaataagac gtatgatcga aataaagcaa
    46201 gaaaagatat acggtttaca agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt
    46261 ctttcgattt atcacacaag ccagacgaaa ttagatgagc ttacgcaatt acagcagctt
    46321 tcgcaaacat tcaacggaga aaatcctaag aagactagtt tggaacggta tgtgcttcag
    46381 gtttatcttc aagaagtgtt acaagtagca aatgatcatc tccaacgttt aacgaaaaat
    46441 cgttatcaat ttgagctagc ggataccatt ggcagttatc gagggaaaac cggattagaa
    46501 atcaatatct atgatgatca ggcaggtatg tctcgaagtg cgcataccct gtctggcggt
    46561 gagagtttta ttgcagcact ttctttagca ttagctcttg ccgatgtgat ccagacacaa
    46621 gctggtggtg tagcaatcga tgcattgttt attgatgaag gatttgggtc attagatgaa
    46681 gaagctttgg aaatggcaat ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc
    46741 ggcatcatta gtcatgtaag agaattgaaa gaacgagtat tacaacaaat aagaattgat
    46801 acagaaggta gcggacaaag caaaatacgc tatcaacttg gataaaaagg gagattggta
    46861 actatgaaat ggagtattcc agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa
    46921 ggccgagtgt tatcggttgt ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg
    46981 ggcagtgaat tgtatatggt tgatttggat ggtacagcaa aagagataga ttactgccaa
    47041 tgtccctatt gggaggaaca taaatattgt aagcatacag ttgctgtgga actttattta
    47101 cggtcgaaaa atctatctcg tatcatgaaa aaagaccagc caatgctaga accggtaaaa
    47161 gcaacaagtg agggagaaat cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat
    47221 cctcaagcgg tgcaaccttt gattattgaa taccaagtag aaacgattga gactaatcag
    47281 tatcacccag agctttctat tttggcactt tacttgcgta tcggttcatt ggcatcaccg
    47341 aaaaaaacat atatcgtaaa aaacatctat gattttttac aagcttacac agaaaaagaa
    47401 acttacactg ttaataaaca gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa
    47461 gatcaaaaaa tactcacgca tcttgcagga agtgctcaga cgcatcaatt attaggtacg
    47521 aacggcttac aagtcaaagg aaaactagat aaaagatatc tgcttctccc agtagaacaa
    47581 gcccgcttcc tgttagagca gatgaatcag actacacgtt tgcatctgca gataaatgaa
    47641 caaaacgatc aaggaataca attttctgat caaaacaaac ctttatcatt caaagtggaa
    47701 aaatttggag aagattattt gctgcaagca ttgaatgatt ttgatttttt ctttatgcat
    47761 tatcagtggg gagttatcca agggacattt tattctctga cgaaatatca acaaacggtt
    47821 tatcaaactt ggaaacaatt gattcgccga ttggagaaac cagaagtagt gttcaagaaa
    47881 aaagaattac cgtctttgtt taaagagatt atccctctgc tgtcagagat cggcgacgta
    47941 gctattgccc ctgcagtaga agaggaagta gcagatatcc ccgtcgaatt tattttcttt
    48001 ttccgaaaag caaaaggaaa aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc
    48061 tattcgactg acccgaagca tgaggtctcg acagaacagg cttttcaggt gttacgtgac
    48121 acagtagaag agaaacgagt acttgatcta ttcagaatgt atcgctacca gaaaaatgaa
    48181 actggttatg aacgtccatt gccaagcggg gaagaactat atgccttttt ccgcacagaa
    48241 ctatctgtct tccgtcagtt tggtgaagtc agacttggaa gaaaattgcg agagttgtat
    48301 ttagatgcgc ataaacatca accgaaaatc gaagtaaccg agaccggttc atggctcgat
    48361 attcgattcg atgtgacagg gatagaggag caagaaatcg atcatgtcct gcaaagcttg
    48421 ctgagaaatg atgcttttta tacattagaa aatggtcagg ttctttcttt tgattcagaa
    48481 gaatttcaac aaacaagcca agtgttgcaa caattacggg aatcgatacg tacagaagaa
    48541 ggaacgatcc atgtacctaa aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc
    48601 aatgctactt ttagtgaatc gttccaaacg atggtacaag atctcattca tccagaaaga
    48661 taccaggcac agcttcccaa aggattgaat gcaacgatga gagattatca aaagcaaggt
    48721 tttcgatggt tgaagatgtt aggtcattat cagtttggcg gtattttagc cgatgaaatg
    48781 ggacttggga aaacactaca gacaattgcc tttttacttt cggaaaaaga agaacggaaa
    48841 agcttctctg ctttgatcgt agcgcctgcg agcttaattt acaactggca ggcagaggtg
    48901 agaaaattcg ccccttcttt aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa
    48961 gaattactgg ctaaagacac agatatccga gtgacttctt atgccagctt aagacaggat
    49021 ctagctaatt atcagtcgca aaaaattgat tatttgattc ttgatgaggc acaaatggtc
    49081 aaaaatagca gtacgaaaac tgcacaagca ttgagagaat tagctgtacc tcagcggttt
    49141 gcactgagtg ggacaccaat cgagaataat ttagaagaat tgtggtcatt gtttgcaacg
    49201 attatgccag gatttttccc gactaaaaca aaattcaggg aacttgccgc agaagaaatt
    49261 gcgcaaatga ttcgaccgtt tattttacgg cgagataaac aaactgtgtt gaaagacttg
    49321 ccggaaaaaa cagagatgaa tctatattct gcacttacag aagaacaaaa aactgtttat
    49381 ttagcttact tgcgtcaaat gcgtgaagaa atcacatcaa tggattctga agcattcaag
    49441 aaaaaccgga tcgggatact tgctggtctg acaagactgc gacaaatttg ctgcgatcct
    49501 cgtctgttta ttgaagatta ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta
    49561 ctggtagctg ccaaagagaa caaacgaaga gttttgctgt tttctcaatt tacagggatg
    49621 ctgactatct tgcaagaaga gttagccgag ctagggatct cgacatttta tttaagaggc
    49681 agtacgaaac cacaagatcg gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat
    49741 gtttttctga tttccttaaa ggctggggga actggattga atctcacggg agcagatacg
    49801 gtcatcttgt atgacctttg gtggaatcct gccattgaag aacaagctgc tggacgtgca
    49861 catcggatcg gacaaaaaaa tgtggtagaa gtgtggcgta tgatctctga aggaacgatt
    49921 gaggaacgca tggattcact ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa
    49981 ggtaatgaag aacagttgac gaaactgact gaagaagaca tccgcatgat tttaagtgta
    50041 ggagaaatcg aggagtaaga gactcgaaaa agcgttcagt aatgggtaag aaagcctttc
    50101 taaagaagcg attgtttatt tgatttaaaa aatagcaagg aggctgggat tccattttat
    50161 cccagcttcc ttgctatcat ttttttattt ctatttttaa aggttataca aagattgtct
    50221 tcattgtctc taacagctga tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt
    50281 caattttgcg ttctcgatgg ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta
    50341 gaacatcatt gtcgtaatta tcgccaacgt ataaggtctc ttctggaagc atatgaaatt
    50401 ctttctcagc cagctgaaaa atctcttttt ctggtttttg gaaacctgtt gcttgagaaa
    50461 tgagcatatt gcgggaagga atccaattgt tcaattgaag ttgcttcagt ttttttgtct
    50521 gatggtcagt cggcccattt gtgataattc ctaatgggac attcttttcc ttcaagaaat
    50581 cgagggtttt tttgacctcc tcatgcaagc agatattatc aagttcttcc tcgtaaattt
    50641 tttgaaataa gaggccatct tcttccgtaa tatgagggta gtctaaatct tttagtgatt
    50701 ggctgatccg atgtgctcgc atatattcta gtgtccaatc tcctgccatt acttttggga
    50761 agttttcatc actatgatgg cggaaccgga tatatagtgg atgcatatct gcatctgaaa
    50821 caagcggaac gactcttttg accgcgtttc gaaatggctg ctgctgatca tacatcgtat
    50881 catcaacatc aaacacaact gatttaatca aaattttaca cgcctttcgg ttttcattga
    50941 ttttttcaca aatccttgac tattgtaaca gaaaaaattg tctaaaaaag ctgataaata
    51001 aaggtttttc ttactatatt tctatttcct tgacagaata aggtttttat atttcagttt
    51061 ttttcactaa aaatcgtgaa acacgtcaat aagtttgatg aacaaattaa tcattatggt
    51121 agaagtcgat tgatttattt taatgcaatc tctgtggaat tttcgaaaga tttatttttt
    51181 taatacagtc tcaatataac tattcaaaca taaaataacg ttatttgatt tatttgtt
//
LOCUS       DARBEG010000011        50604 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000011 DARBEG010000000
VERSION     DARBEG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.011"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(16..1380)
                     /locus_tag="SCK20_000627"
     CDS             complement(16..1380)
                     /locus_tag="SCK20_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1144718.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1377..2936)
                     /locus_tag="SCK20_000628"
     CDS             complement(1377..2936)
                     /locus_tag="SCK20_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144719.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(2992..5472)
                     /locus_tag="SCK20_000629"
     CDS             complement(2992..5472)
                     /locus_tag="SCK20_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144720.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(5456..5791)
                     /locus_tag="SCK20_000630"
     CDS             complement(5456..5791)
                     /locus_tag="SCK20_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144721.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5805..6839)
                     /locus_tag="SCK20_000631"
     CDS             complement(5805..6839)
                     /locus_tag="SCK20_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1144722.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6929..7609)
                     /locus_tag="SCK20_000632"
     CDS             complement(6929..7609)
                     /locus_tag="SCK20_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1144723.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7893..8996)
                     /locus_tag="SCK20_000633"
     CDS             complement(7893..8996)
                     /locus_tag="SCK20_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1144724.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9625..10095)
                     /locus_tag="SCK20_000634"
     CDS             complement(9625..10095)
                     /locus_tag="SCK20_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG1144725.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10425..12065
                     /locus_tag="SCK20_000635"
     CDS             10425..12065
                     /locus_tag="SCK20_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG1144726.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12219..13541
                     /locus_tag="SCK20_000636"
     CDS             12219..13541
                     /locus_tag="SCK20_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG1144727.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13609..14043)
                     /locus_tag="SCK20_000637"
     CDS             complement(13609..14043)
                     /locus_tag="SCK20_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1144728.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14108..14945)
                     /locus_tag="SCK20_000638"
                     /pseudo
     CDS             complement(14108..14945)
                     /locus_tag="SCK20_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(15687..16313)
                     /locus_tag="SCK20_000639"
     CDS             complement(15687..16313)
                     /locus_tag="SCK20_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144729.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(16608..16943)
                     /locus_tag="SCK20_000640"
     CDS             complement(16608..16943)
                     /locus_tag="SCK20_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1144730.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(16930..17214)
                     /locus_tag="SCK20_000641"
     CDS             complement(16930..17214)
                     /locus_tag="SCK20_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1144731.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(17270..18793)
                     /locus_tag="SCK20_000642"
     CDS             complement(17270..18793)
                     /locus_tag="SCK20_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1144732.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(18786..19961)
                     /locus_tag="SCK20_000643"
     CDS             complement(18786..19961)
                     /locus_tag="SCK20_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1144733.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(19965..20150)
                     /locus_tag="SCK20_000644"
     CDS             complement(19965..20150)
                     /locus_tag="SCK20_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144734.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(20116..21810)
                     /locus_tag="SCK20_000645"
     CDS             complement(20116..21810)
                     /locus_tag="SCK20_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1144735.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(21807..22280)
                     /locus_tag="SCK20_000646"
     CDS             complement(21807..22280)
                     /locus_tag="SCK20_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG1144736.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(22349..22504)
                     /locus_tag="SCK20_000647"
     CDS             complement(22349..22504)
                     /locus_tag="SCK20_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG1144737.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(22638..23018)
                     /locus_tag="SCK20_000648"
     CDS             complement(22638..23018)
                     /locus_tag="SCK20_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1144738.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(23022..23237)
                     /locus_tag="SCK20_000649"
     CDS             complement(23022..23237)
                     /locus_tag="SCK20_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144739.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(23240..23647)
                     /locus_tag="SCK20_000650"
     CDS             complement(23240..23647)
                     /locus_tag="SCK20_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG1144740.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(23913..25388)
                     /locus_tag="SCK20_000651"
     CDS             complement(23913..25388)
                     /locus_tag="SCK20_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG1144741.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(25378..26226)
                     /locus_tag="SCK20_000652"
     CDS             complement(25378..26226)
                     /locus_tag="SCK20_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG1144742.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(26263..26625)
                     /locus_tag="SCK20_000653"
     CDS             complement(26263..26625)
                     /locus_tag="SCK20_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144743.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(26626..26784)
                     /locus_tag="SCK20_000654"
     CDS             complement(26626..26784)
                     /locus_tag="SCK20_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144744.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(26925..27549)
                     /locus_tag="SCK20_000655"
                     /pseudo
     CDS             complement(26925..27549)
                     /locus_tag="SCK20_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(27546..27857)
                     /locus_tag="SCK20_000656"
     CDS             complement(27546..27857)
                     /locus_tag="SCK20_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144745.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(27901..28194)
                     /locus_tag="SCK20_000657"
     CDS             complement(27901..28194)
                     /locus_tag="SCK20_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144746.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            28388..29035
                     /locus_tag="SCK20_000658"
     CDS             28388..29035
                     /locus_tag="SCK20_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1144747.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            29096..30241
                     /locus_tag="SCK20_000659"
     CDS             29096..30241
                     /locus_tag="SCK20_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1144748.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(30322..30627)
                     /locus_tag="SCK20_000660"
     CDS             complement(30322..30627)
                     /locus_tag="SCK20_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG1144749.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(30652..31083)
                     /gene="ruvX"
                     /locus_tag="SCK20_000661"
     CDS             complement(30652..31083)
                     /gene="ruvX"
                     /locus_tag="SCK20_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG1144750.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(31080..31346)
                     /locus_tag="SCK20_000662"
     CDS             complement(31080..31346)
                     /locus_tag="SCK20_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG1144751.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(31601..32326)
                     /locus_tag="SCK20_000663"
     CDS             complement(31601..32326)
                     /locus_tag="SCK20_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1144752.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(32687..32757)
                     /locus_tag="SCK20_000664"
     tRNA            complement(32687..32757)
                     /locus_tag="SCK20_000664"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32723..32725),aa:Gly,seq:ccc)
     gene            32874..33626
                     /locus_tag="SCK20_000665"
     CDS             32874..33626
                     /locus_tag="SCK20_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1144753.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            33642..34121
                     /locus_tag="SCK20_000666"
     CDS             33642..34121
                     /locus_tag="SCK20_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1144754.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(34372..35952)
                     /locus_tag="SCK20_000667"
     CDS             complement(34372..35952)
                     /locus_tag="SCK20_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG1144755.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(35945..36688)
                     /locus_tag="SCK20_000668"
     CDS             complement(35945..36688)
                     /locus_tag="SCK20_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144756.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36864..37640)
                     /locus_tag="SCK20_000669"
     CDS             complement(36864..37640)
                     /locus_tag="SCK20_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1144757.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(37888..38724)
                     /locus_tag="SCK20_000670"
     CDS             complement(37888..38724)
                     /locus_tag="SCK20_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG1144758.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(38735..39664)
                     /locus_tag="SCK20_000671"
     CDS             complement(38735..39664)
                     /locus_tag="SCK20_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG1144759.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            39856..40209
                     /locus_tag="SCK20_000672"
     CDS             39856..40209
                     /locus_tag="SCK20_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1144760.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(40283..40939)
                     /locus_tag="SCK20_000673"
     CDS             complement(40283..40939)
                     /locus_tag="SCK20_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1144761.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            41165..43099
                     /locus_tag="SCK20_000674"
     CDS             41165..43099
                     /locus_tag="SCK20_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1144762.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(43185..44408)
                     /locus_tag="SCK20_000675"
     CDS             complement(43185..44408)
                     /locus_tag="SCK20_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1144763.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(44402..46300)
                     /locus_tag="SCK20_000676"
     CDS             complement(44402..46300)
                     /locus_tag="SCK20_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG1144764.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(46294..47412)
                     /locus_tag="SCK20_000677"
     CDS             complement(46294..47412)
                     /locus_tag="SCK20_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1144765.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(47495..48328)
                     /locus_tag="SCK20_000678"
     CDS             complement(47495..48328)
                     /locus_tag="SCK20_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1144766.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(48321..49238)
                     /locus_tag="SCK20_000679"
     CDS             complement(48321..49238)
                     /locus_tag="SCK20_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1144767.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(49465..50589)
                     /locus_tag="SCK20_000680"
     CDS             complement(49465..50589)
                     /locus_tag="SCK20_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1144768.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        15057 a        10217 c         8542 g        16788 t
ORIGIN      
        1 agaccgtgat atttctcaca gtcttttatc tactctattg ctatttgctc tcttagctag
       61 tactttaaaa gctaactcat tgaggtagtg aaaacgagag gtgtgttcag acagaaatcc
      121 aaaatcctca actgctagct gtggaatcgt agttttgtgt ttcacgatat ccgcaagcag
      181 gttcaaatct gataataaga attcttggct tatcagctgt ccgccaatta tttctaatgt
      241 atccggatgg tagatcagct ttgcatttac tgttttgttt tcttcatcgt attgtggaaa
      301 tagatcaaat tctcctgaac acgaaccggt attttgttcc aagaatggcg cttcatcttc
      361 tgtcagccct acagttccta aaaagtagtc tataatgact gctgctgtta tcttttgtct
      421 gaggtcgtaa gcggttggct gaccactcaa agttagcgct actgctcttg ccatatgtac
      481 agctcgagta actaaaggta aaaagagatg ccgtttattt ttgtcaaaac tgactgggac
      541 gatatctcca atcgcataga tgtccggatc acttgtctgc agatattcat tgactatcac
      601 agtctcatcc tcatacaaag ccactttttc ttttaacaat gtcgtattag gacgagtttg
      661 ggcagaaaaa atgaccatat ctgcctttat tgcttgagat agaagttgta cggatactac
      721 tttctcttct gattttttcc aatcgatcaa gaattcattt ggataaaaat tgatttgctt
      781 agtttgcagc tgattttgaa aactgttgat catatcctta tcgaaatatc ttgataaaac
      841 attcggcata taatcaacta gatgaataat gtatccttta tttctcaaag catctgctaa
      901 ttccaatcca atataaccag caccgaagat cactacttct tttgcttctt ttaaagcggc
      961 aagtacttgc ttagattgca ctagattttt ataggtgtag acattttcag cttcaatttg
     1021 tttatacaag gtgacaggag aggaacctgt tgctacgatt aatttatcat aggttatggt
     1081 ctcgtttttt ccttctttgg atgcgaggat gagctgatgt ttgtctggat caagttcagt
     1141 tactctccat tcggtgatca gttttgcccc actttcttga agagtccgca tgttatgggc
     1201 aacttcagct aaactacgta tctttccttt taaataaagg ttgattccat ttgaaacata
     1261 gcctagtcca tctttatgat cttcatcgat cagtaaaact tctgctttcg gttgaagttt
     1321 tcttagattt agggcagctg tgatgcctgc atgggaagca ccaatgatca cgattttcat
     1381 gttttattca tccttaaatt caatttttct aaataacatt cttttaaaaa ttggttcaaa
     1441 taggggaaat cataattata ttcgttgcta gtgaatacaa aaactttagc ggacttctgt
     1501 gacgggtact ctttcaattg gctagtcaaa atgatatcat actgcttttc tttctcgtat
     1561 tcgactaaat catatttgat cgtattcagc aattgttcct ggatgatttc tttcacgata
     1621 tccgccatta gcctttcata aggaagatca taagcaattt gcagacgtaa ttctaactgc
     1681 ttgatcagcg tccgcaaaat agatgcatag cgagcaaata attcaaagcg ggcattttcc
     1741 tcgtctactt cagtaaaatc aacaatagca aatgtttgtc tgaccaatct ttcacataaa
     1801 accaggaatt gtttatcttc tacttcagtt aaccgctgtt ttaatccttc ttggccaaaa
     1861 atattcatcc atcctctaaa ataggcaaaa tgagcgtgat tttgcatcac tgtcaacgca
     1921 atcttttgcg aaatctcttc tggaaaaaca taaaaagaga aatattcttc tatcgattgt
     1981 ttaaacgttc gattcaattg agctactacg tcgtctttgt atttttcgct tatcaaaaac
     2041 tcctggacat gcgagttatg tgtatctaat gtaaagttac tgatcatgaa aagataaaaa
     2101 accaacgttt cattttgatt ccaaggaaag gcatattgaa agaagtaacg ggtcaaaaat
     2161 gtatggaggt ccaataaaaa ttgtgtcgtt tgaaacaatc caacaggaag attgctataa
     2221 tctggtttgg attttttcgt acagcgcatt cgcttcttca taatggaaag ccatatttca
     2281 aatttcaatc gtccttcttc tgtaatagta aaactaaact ctttttgcag cagatctaaa
     2341 aaagtgtttc ctgataaatc actgtattct tttttgtttt caagataagg acgtccaaac
     2401 caaaataatt ggaaataaaa ataacaaatt tgactttctg gaccggtcag ccgcccgctt
     2461 tttattttca gatgaaactc atttaaagaa tcatttaatt ccttcactct gcgagacaaa
     2521 gtagccgaac tgatattaag ttccatgaca agtttctgcc gatcaaatga gccattttcc
     2581 aacagataaa gaagtatctg atattttgta gaatagcgca agtagcaatg ataataaaca
     2641 tcgactgctg ctatatttcc atgtctgatt aataaaatat tcccttcttg gataacaatt
     2701 tccaattgtt tgatattttc caagttagct tgtaaataaa acagatcttc tttcaaggta
     2761 gcccgatcaa ctgataagcg ggtaagtacg ttattaacgg aaatctctcc tttttcctct
     2821 aataaaagtt ggaaaagttt gatttgccgt tgttcatttt tttcaagcaa actgaaaagt
     2881 tccaagtcca ctcctccaag catttctgat tttaacagca gaagtttcct tctcatcctt
     2941 gaaaaagagg atctgtgaca gttatcacag atccatacaa attctaatca cttagctggt
     3001 tggcccagct gtcagttccc atgtgaccat gccttgatac gttgcgccag gttgagcatt
     3061 cgtactgcta tttacctcta agagtaaacc atcggtatcg ggaaagtcaa cgagaacctc
     3121 tcctttagct gttccatcac tctcataaat caacgtgttt tgaccttgct gaatcaattg
     3181 ttttgatcca gaatgattga agtataattt tgttccgctt aattcttgac cttgatcatt
     3241 cttaaataaa gaagtcaatg tccctgtcag ttgccaagga gacgtgcttg gaagccgagt
     3301 atcccgcaca tcaaaagaaa aatcgccttt tgcttcataa taacttgtct tcaacggttt
     3361 ggggagatcg ttaccaaatg taaaatcatc tggtacatta actaatgtta attcttgttt
     3421 ggggacaaga attgttttag cagatgtttg gacaactggg ttaggaagct tagcaccatc
     3481 tgctaatgta atattgctgg aaagtacagc tgcctttgtc agtatgctga cggattgtac
     3541 agaagtatct acagtattta attgatagtt gtaagtaaga tgaatggtat ctcctatttt
     3601 gctcaataga tctgctggga cagaaactcc ttgttgaatg ccggtatctg cgatagttgt
     3661 accatttaat tttaaggaac cattgatata agtagtattt tcaggaagtg agatcgtgat
     3721 ttttttgtcg gttatcgcat ccccagattt tgttaacgtg aatgtcacat tttcttgaat
     3781 cgtatctcct gatagagcag acaggggatc tcctgtctca ggttgtctgc taaggttttt
     3841 tagctgacta tctgcactca cagatggaaa attagccact gttatttgag gaagagtggt
     3901 gattccagct tgttcagact gccatttgta cgcaaaagac ggatacatgt tagtaagtac
     3961 atcgtccacc aagcgataat caaaataata ttgccctgaa gccaatgcag gtagtgtaga
     4021 agtagctgta tttacacccc cgttatttga ttggatcgtt tggaaaagtt gatctgtcca
     4081 tcctgtcgct aatttttggg ttcctttcga ataaagcctg taatggatcg aacccttttt
     4141 tagttcagtt gctgaccaat tggccgtgac ttggtacgat gcagaagaaa ggagctttcc
     4201 ccactgacta tcggctgtta agtttgttgg taaaaccgtt ccgtctagct tgtactgctc
     4261 caataccttt aaagcaacag gagaaagcaa tccgccttgc gcatctttcc cactgtacac
     4321 gatattggct gtctgattca atccacttgt gcttgctgga gtatgagtca gtattgctcc
     4381 ttgtgtaacg ttttttcctg taaaatagtt gactacgcga tcaaagcgac taaatggcaa
     4441 ctgatcaaaa cgtccttcag tcagccccat cgtcaatgtc cgactattgg tagagcctgg
     4501 aaggttctgg ttatttccat ccacgtattc tgtggtatca gcaaatgtat tatattttcc
     4561 tgttgaatca cttgttgaga tgtaactatt ccacgtattt atctctgcac ttgcagaaaa
     4621 tgcatatgct cctggattca agtttccgcc agttaattta ggataaaatc tacctgcagc
     4681 tggagctaaa atcgaattga acatacgatc ttggataata ttattcgaat cataaaaatc
     4741 aacagaaaaa acggcctttt tagacataga aggttgtaat ttacccttgg tcaaaatttt
     4801 gtttggactc gtatcatatt tatctccgtt ttctgtcatc ttcgcgacat tagctgtaac
     4861 atcaaattga agcgggatat cgtccacgtt gaattcgcct cgcgtaataa tagcaataat
     4921 atctgccaat agagtatttg tatcccacga ccccatccca aaacgcaagg tatggcttcc
     4981 tgctgataat aaactaccgc tagtaacctt gatgggggca ccggcaatat acaagtaggc
     5041 gttgttccag tcaattgctt ttaatacatc actgcttgat acatgttccg gcaaattgat
     5101 gatcgccaca aatgcaaatc cttgtgcagt gatattcacc aaatcaagta catttgttag
     5161 tttcacatgg ctttcccaat gaaaacggac cgtgccacct ttccaaggat catttgaacc
     5221 atcgttcaaa taagtagcag tcaatttagg caattggtta gccccagtta gttggacaag
     5281 cttcccgcca ttgactgcac tacgtccacc atttggaggt acgatatttt catacgtgta
     5341 tggatcataa gaaatggcag gaggcgttgc tccaacagct tctgccgaag gtgaaaagat
     5401 aaatatactt aaaacaaaaa gcgaagtaaa aaagaaggag cgaaaaaatg aaaatttatt
     5461 ttgtagcaac atgttcatcc tccttttcct ttcttctcca taacaacaac cacgcaagaa
     5521 gccataattc tataccaatg atcaaataaa gaaaacggat cttttcttca ttcgtctccg
     5581 ggaatacgcc atgttgatta gattctttat aaatcggtag ctgtgtctgc tgttttgatg
     5641 cgccattgac tgaaccactt ggatgcggaa ctggacttag atcataaaat gtaatcgtac
     5701 ctgaactttt ggtatctgct tggactggct cttgccagct gaagaacagc aaacttgcaa
     5761 taatccctcc caataagatc gatgttttca acgaaatcac ctcatcatgt tgtttctcct
     5821 tttttctttt tcttgtggac ccacaaaaac agcaaaatca accataacac tagaactgca
     5881 gcaaccaatc ctgcataaag ccatgggaaa gattgttctt gtactgcaaa aacggaattt
     5941 tcatttactt tttgtgcttt ttcttttttg atcgtaaagt tttgtgtaaa tcgccattca
     6001 ttactgtcgc ctgattttgc tatgatttct gcgcggtaat cgccggcttt tgctttatca
     6061 ccagctaaat caagattcaa gtgaaaattc gaatttggtg caactaaaaa ttcattagac
     6121 gtattttcaa tataaagctg attttcataa aagatcttgc tcgtaaatat caaattgctg
     6181 ataatcctag gctgaggatt ttggaaacga agctgaacag ttggctttgc gttgacctcg
     6241 tccaaatctc ctgataaaag tttcagatcc ggttctatcg tctcttgact ttcttgtaaa
     6301 acgactgcga gattataagc aatacgattt ttgatttgtg ctccttctgt agcattttct
     6361 gtttcagctt cttctttagg tgaaacatga atacccgcta atacacgtcc cttaaagctt
     6421 tttgctggga ttttcacttc tataggtata gttgtttctg catgtgctga tacattgatt
     6481 tcctttttcg caatgagcaa aacgtcacgg acatcaaacg gcaatgtctc atctaacgtt
     6541 gggttttttt gactataatc gatcgttcca ccatcactcg ttaccgccgg attgacagag
     6601 acctcaaatg ttcgttcttc atctgaagaa ttgctgataa ttacttctag tgtctgattt
     6661 tgttccggtg ccactaataa atcaaaataa ccgattgctt tatctatttg gttctctgga
     6721 aataccggtt tgattccaaa gtctcctgct gaatcctttg cttggctgat tgacggaaaa
     6781 atgaaaaata gacttaaaat agaaaaaaac gaaacaaaca gtcgtttact ttgattcatt
     6841 gtccaccaca tcctttcaac aacaactgcg gtaaaaaatc cttcacctat agtattttca
     6901 ccgcagtttg ttgaatttat ttcatactct atgctactgg gccagccacg aggttccaag
     6961 tcaaatcagc cgtgaatgtt ccagctgctg ctttcggcac tttcagtgtg atatttgctg
     7021 gatcataaac agaaagccat gttcccagac cttgatcttt tgctgctgta aatattggtg
     7081 tcgctgcact tgctttactc aacccagtta cagtttgact agttggtcca gtggattcgt
     7141 tatctgacgt acttttaaca gtggatgttc caaaatctaa ttcagcgcct tgcaaaactt
     7201 gtgaaccgtt tacaaaatct gaaaccgaaa ctgtcacgtt ccacccttgt gcttggccat
     7261 ctgcacctac acctcgacga tctgatactt ggagataagg tgtatcgttt tttgaagcgt
     7321 ctacttgata cgtttttgtt ccgctttcaa tctcgtgtgt accaaatggc aattcaggaa
     7381 ctacatccaa tgttaaagaa cctgtctggc cagttgctgg attttccgga tctgctggct
     7441 ctactggttt tgaaggatca gtagggttaa gaggctcaac ggtatttgtt ggttcagtaa
     7501 aggtaatatc tccttttgaa gtcgcttgac cctcagcaaa agctggtgtt gccaagccgc
     7561 ctagaagaag tgttgaaagt aataatgtcc caatcatgtt ctttttcata ataaatcact
     7621 cctttttctt agtgacctaa gtatagtaga gacagtatgc aaaaaatcct catgaattca
     7681 tccgcttact tgaaattatg accctttttg tataaaagag aaaaaaacaa taaaatcgtt
     7741 tacatgaata ttcttccttt tttattaagt atgaaaaaat atttcaattt tattttttcg
     7801 tttcaaatta ttctattagt gacatccttg aactataaaa ataggagtac ggcatcatct
     7861 gccgcactcc tatttttttc gtcatttatt tcttaaataa aacgattctc acgcaatttt
     7921 atttttttga tatcttctac tgtttgtgtt ccagctaatt gcatgaccat ttccaattct
     7981 gttttaaaga aatcaaacac ttgatggact cctgtacttc ctcctaaaga caatccataa
     8041 ataacgggtc tgccaatagc aacaagatcg gcacctgatg cgattgcttt gaaaacatgt
     8101 tgtccacgtc gtacaccgct gtcaaacacg attggtacac gtttatctac tgcttctgcc
     8161 acatattgca acgaatcaaa cgcagcaggc cccccatcta attgacggcc accatggtta
     8221 gatacccaaa ttccaccagc accagattct aatgatcgat agacatcttc ttctgattga
     8281 acacctttca cgtagactgg caaatcagaa tgagcagcga taaattctac atcttttgga
     8341 ctaagttttt gtttggaaga tttatataca gcatccatcg tttggcctac tcctgattga
     8401 taagcctgaa cgatcggcat tggcagcggg aaggtaaacc cgtttctgcg gtctgtttca
     8461 cggtttccgc caactgttgc atccgctgtc aatacgattg cttttgctcc attacgcttg
     8521 gccacttcaa gaatatccaa attgattcca ttatctttgc tcatataaaa ttgaaaccat
     8581 tgcggtgctt tctccccgcc tgcttctctt atctcttcta aagtacaaga agcgtaagaa
     8641 cttgctgtat agatcgtccc aaaatctgct acaccttttg cagaagcttt ctccgccttc
     8701 acatgagcta atccatgagc agcaactggt gccatgatga ttggtgcagt cagcatttct
     8761 tcgtcaaaat gtgtagttgt atcgggtaat tctacatctc ttaatacatg aggaataatc
     8821 aaacgatgat tgaatgcccg ttcattttct tgataagtaa ataagtcgcc agcaccactg
     8881 ctgatgtaac cataaccacc agctggaatc acttgtgaag ccgcgatttc taaatcttcc
     8941 atattgataa aatctactat tccttcattt gtacctgctt gatatgtttt ttccatctca
     9001 atgccttctc tctataaaat ttgaatgatg aacaatatga tagcgtttac actttttata
     9061 ttactcttcg acattgatac aggcaaataa tctgtttaca tttcagccat tccagatgtc
     9121 actactctgg aagaaatgat tgtaataaaa atagataaaa aaccgatggg actttgacaa
     9181 aagtagtgtc aaagtccctt tttttgaata aacggtgttc aaaagccatt ttctcggtat
     9241 taagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc ttactactcg
     9301 aaactaagcg actttttcac aacctctcga taaaacggtg ttcaatcagc tgaccctcgg
     9361 tattagctca aaaaatggca aaatatgagg aactattttg ccatttgtct ctctaatact
     9421 caggtcagaa cgttgacttc acagtctctt ccttaaacgg tgttcaaaaa cttgcatctg
     9481 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9541 tacttgatgc agggcaggtt tttcacgacc tctgattcac ggtgtttctt tttcatcaac
     9601 atttttacga aatactgtgg aagactattg aaataagtgt ggattgtttc tttgtgtttc
     9661 aatcgtttct tgccattctt catttaattt tggattaccg ttttcttcct tgaactcata
     9721 aggtcctaat tcaaaaatcc cagacagtat ttgattcacg atttcatata gttcagtgtg
     9781 atcagacaca tgattcttga agatcatctc attatcacca cgtttgaacc tcacattcgg
     9841 gctctcatga tgaacctcta tgcatttttc tccttttttt aactgacgga cttctgaaaa
     9901 catctcataa atatcttctc cagtaaaacc ttcagcaagc gccagactgt ccacaagacc
     9961 gcctactgtc agttcagaga ttattgcttg attggaatct aattttaatg tatagcttgt
    10021 cgcttctact acgccacatg cattgcaacc gccttctgat agttcgcttt ttctaatcaa
    10081 ttgaacttct ttcattttct cttcctcctc ttaattttct acttatgtat ttttatcacc
    10141 tgacataggt gactcctatc aacgctatca tgtgcttttt tcctcagaaa tcctatttat
    10201 ccattccatc tatcccttat tcttctactt ttctttctct agcatataag agatacgcat
    10261 agtattcaag caaggattgt cgatataaaa agaacaaata ctgctatttt gatttctttt
    10321 ggctatatta catataaata aaatttatca tacctgctct ttttgactat atcatcatct
    10381 gacttctata cttcgtttgt attaaaactt aggaggaatc attcatgctt acagggttaa
    10441 acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa gaacgggcaa
    10501 aatatggaat cactggtatg cttccaagta ctgtccaaac actggaacaa caatctgttc
    10561 aagcttatgg tcaatactta agcaaacaaa cagatttgga aaaacgtatc ttcttaatga
    10621 atctattcaa tacgaatcgg acattgtttt ataaattgat gggcgaacat ttagttgaat
    10681 tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac aacgaaattt
    10741 tcttgaaacc gcaagatgct gcatttcttt caatcgacga accagaaaac atcaaagcaa
    10801 gtttgaagaa cgcggcagat ggacgagaca ttcgtttgat tgttgtgact gatgcagaag
    10861 gaatcttagg tatgggcgac tggggtgtga acggtgttga catcgctatc ggcaaattga
    10921 tggtctatac tgctgctgct ggtattaatc cagcacaagt tttgcctgta tcgatcgacg
    10981 ctggtactaa taatgaagaa ttattgaata atcccttata cttaggcaat cgtcatgctc
    11041 gtgtagaagg cgaaacttac tatgaattca tcgatcaatt cgttcaatca gctaccgaat
    11101 tattcccaga attgttattg cattgggaag attttggacg aggaaatgct gcagcgatct
    11161 tagaaaaata cgaagatcaa atcactactt tcaacgacga tatccaaggt acaggaattg
    11221 ttgtcctagc aggtgtatta ggcggattga atatatccgg tgaacaattg aaagatcaaa
    11281 taattttgac atttggtgct ggtactgctg gtgtgggtat cgcaaacatc cttttagatg
    11341 agatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat caagtagata
    11401 aacaaggttt attgtttgaa gatacagaag gattgactcc tggtcaaatc ccattcgctc
    11461 gcaaacgctc agatttcact aatgctgatg aattgacaaa cttagaagca gttgtaaaag
    11521 cgatccatcc gacaatcatg attggtactt ctacccaacc tggagcattc actgaagaaa
    11581 tcgtgaaaga aatggctgct catacaccac gtccgatcat catgcctttg tctaatccaa
    11641 caaaattggc agaagcaaaa gcaaaagatt taatagaatg gacggacgga aaagcattag
    11701 ttggaacagg tatccctgct gctgatgttg aatataatgg tgtaacttac caaatcggtc
    11761 aagcaaataa tgctttgatg tacccaggtt taggacttgg tttgattgct tcaacagcta
    11821 cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga atcgttgatg
    11881 taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa ttttctcaaa
    11941 cgattgctga aactgtcgca aaatcagttg tcgcacaaaa cttgaatcgt gaagaaatca
    12001 cggatatcaa agaagcagtt gaatctgcta aatgggttcc tgaatataaa tctttagaag
    12061 attaagatcg ttatagtaaa agaacataca gaaactttgt atgacgtcta aaaagaaaaa
    12121 agcgactgcg gcaaaacagc ggccagccaa ctcctgctct tttgtcgctt ttctttggct
    12181 tttttcatat gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa acaacaactg
    12241 caactgcagt tgaggaaaag aaaaaagaag gtttttggtc cacaaaaatc accggcgtaa
    12301 gtttgccact ttatttgatc atggtccttg tcctgatcat tgcaatggct ttagggaaat
    12361 taccgacgaa tatgattgga ccaatcgcag ttttggttat tttcggtaat ttatttcatt
    12421 ttatcgggac aaaaatccct atcgtcaaaa gttatttggg tggtggttcc gtttttgcga
    12481 tcttcgcttc tgctgcgatt gctacatttg ggatcttacc tgaatatgta gtgaaatcca
    12541 cagaaaactt tgtcagcaat atgggctttt gggactttta tattgctgcc ttgattactg
    12601 gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt tttattcccg
    12661 ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtgggc atgcttatcg
    12721 gaaatggttt tgcaaattcc gttttataca tctcattacc tgcgatggct ggtggtgttg
    12781 gagccggagc cgttccttta tcaacgattt atgcaaatgt tttaggtacg gatgcttctt
    12841 ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcttagct atcatcggtg
    12901 cagcattagt tgctcgtgct ggacaatcaa tgccaaaatt caacggcaat ggtaaattaa
    12961 tggaaaaagg cgatcttggc gatggaaaac ctcgaactgt gaaaccttcc attccacagt
    13021 taggtgttgg tttgatggta tcattgactt tcttcatctt aggacaaatt ggtaactatt
    13081 ttgttcctaa agtccatgct tacgcattta tgattattat cgttgtcatc tgtaaaatcg
    13141 ccaacctatt accagagtat tatgaagatg cagctatcat gttcaataac ttgattgtca
    13201 aaaacttgac agcagctgtt cttgccggta tcggtattgc gttgctgaat ctgaatgtat
    13261 tagcttctgc tttgacatgg caatttgttg tcttatgtct gacaagtgtt atcgcgattt
    13321 cagttgtttc aggatttgtc ggtcgtttgt ttggtttata tccaattgaa tcaactatta
    13381 ctgctggctt atgcaacaac tcgatgggcg gaacaggaaa tgtagccgtc ttatctgcag
    13441 ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcgacttggt ggtgccatcg
    13501 ttttgatcat ctctggtttc ttgatgcaat tactttctta gtcaaaaaaa caaaaatacc
    13561 tgatcatcaa gtatcggctt ggtgatcggg tatttttttg ataaagattt acaggatttt
    13621 caaaaatctt ttttgcattt tatcggaatg ataaccgatg tgttcataaa attggtgtgc
    13681 ttctactctg tattctgcag aattaagcct tattgtcgtt attcctcgtt ttttcgcttc
    13741 ttcttctacg ccagtcatca aagcctttcc gatgcctctt ttttcagcat atttagacac
    13801 agcaagtgcc ataatattga acattggatc gctaaaaatc gattcatagg actccgcttg
    13861 tacatagcca gctgcttttt tagaagtagg atcttcataa accaagaaaa aatttttctc
    13921 tggctccttc agcagtttct tcatttgtct caccgtaacg gttaatggta tatcataccc
    13981 taattgatcc gcattgattt cacgtagtgc ttctgcatct tctaaacgca tttcccgtaa
    14041 catccaaatc accagccaat ttctgatttt ttaaaatgaa tttttttatc atagtatact
    14101 ctttttttca tttagaacaa tatgtttcag tgattttgac ttgcgttagt ctagattctc
    14161 ctaatgtttg taagatgaca aaggcattgt aacaattgac caaaagaacc acgaggacaa
    14221 ttacttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
    14281 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
    14341 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
    14401 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
    14461 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccca
    14521 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
    14581 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
    14641 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
    14701 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
    14761 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
    14821 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
    14881 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
    14941 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
    15001 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
    15061 agtgctaaac gaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca
    15121 aacaaaaata gctcctcata attttcgttt ggttgaactt attaccgcag atgcaagctt
    15181 ttgaacaccg tttaagaaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
    15241 aatttcaaaa aacagcttct catgtttgtt gaatattgtg acttgtgacc gaggagttgg
    15301 caggagaaca ccgtttaagg aagagactgt gaagtcaacg ttttgacctg attattagag
    15361 aaacaaatgg caaaatagtt cttcatattt cgccgttttt cgagcgaatg ccgaggggca
    15421 gctgattgaa catcgtttat ttggaaaaag ggattgtgac aagacgtttg tcacaatccc
    15481 tttttttata tcttattaat tcattctttt gttctattct tcttcttccg ccatgaatga
    15541 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
    15601 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
    15661 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
    15721 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
    15781 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
    15841 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
    15901 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
    15961 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
    16021 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
    16081 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
    16141 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
    16201 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
    16261 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
    16321 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
    16381 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
    16441 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
    16501 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
    16561 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
    16621 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
    16681 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
    16741 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
    16801 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
    16861 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
    16921 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
    16981 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
    17041 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
    17101 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
    17161 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
    17221 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
    17281 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
    17341 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
    17401 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
    17461 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
    17521 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
    17581 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
    17641 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
    17701 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
    17761 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
    17821 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
    17881 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
    17941 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
    18001 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
    18061 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
    18121 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
    18181 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
    18241 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
    18301 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
    18361 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
    18421 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
    18481 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
    18541 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
    18601 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
    18661 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
    18721 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
    18781 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
    18841 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
    18901 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
    18961 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
    19021 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
    19081 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
    19141 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
    19201 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
    19261 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
    19321 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
    19381 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
    19441 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
    19501 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
    19561 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
    19621 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
    19681 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
    19741 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
    19801 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
    19861 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
    19921 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
    19981 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
    20041 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
    20101 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
    20161 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
    20221 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
    20281 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
    20341 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
    20401 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
    20461 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
    20521 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
    20581 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
    20641 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
    20701 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
    20761 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
    20821 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
    20881 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
    20941 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
    21001 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
    21061 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
    21121 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
    21181 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
    21241 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
    21301 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
    21361 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
    21421 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
    21481 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
    21541 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
    21601 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
    21661 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
    21721 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
    21781 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
    21841 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
    21901 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
    21961 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
    22021 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
    22081 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
    22141 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
    22201 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
    22261 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
    22321 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
    22381 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
    22441 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
    22501 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
    22561 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
    22621 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
    22681 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
    22741 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
    22801 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
    22861 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
    22921 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
    22981 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
    23041 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
    23101 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
    23161 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
    23221 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
    23281 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
    23341 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
    23401 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
    23461 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
    23521 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
    23581 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
    23641 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
    23701 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
    23761 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
    23821 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
    23881 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
    23941 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
    24001 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
    24061 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
    24121 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
    24181 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
    24241 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
    24301 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
    24361 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
    24421 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
    24481 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
    24541 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
    24601 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
    24661 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
    24721 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
    24781 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
    24841 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
    24901 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
    24961 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
    25021 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
    25081 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
    25141 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
    25201 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
    25261 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
    25321 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
    25381 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
    25441 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
    25501 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    25561 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    25621 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    25681 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    25741 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    25801 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    25861 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    25921 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    25981 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    26041 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    26101 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    26161 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    26221 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    26281 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    26341 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    26401 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    26461 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    26521 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    26581 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    26641 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    26701 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    26761 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    26821 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    26881 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    26941 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    27001 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    27061 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    27121 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    27181 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    27241 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    27301 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    27361 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    27421 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    27481 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    27541 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    27601 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    27661 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    27721 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    27781 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    27841 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    27901 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    27961 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    28021 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    28081 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    28141 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    28201 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    28261 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    28321 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    28381 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    28441 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    28501 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    28561 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    28621 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    28681 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    28741 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    28801 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    28861 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    28921 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    28981 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    29041 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    29101 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    29161 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    29221 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    29281 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    29341 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    29401 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    29461 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    29521 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    29581 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    29641 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    29701 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    29761 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    29821 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    29881 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    29941 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    30001 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    30061 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    30121 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    30181 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    30241 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    30301 ggtttctcct agctttatag tctattcttc ttcttccgcc atgaatgtat tgaatacttc
    30361 ttcaatcatg tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc
    30421 ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc
    30481 aggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagtaggat
    30541 ttcatataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcatg
    30601 gtcgtgggaa tggttatgtt ctgtcataag attacctctt ttcttttatt attaaaataa
    30661 ttgcttacct gagccgtcta aataattttg taagatcatc acagctgcta atttatcgat
    30721 gacttttttt cgtttggctc ttgaagtatt tgcctgctct actagcatac gttctgcctg
    30781 aacagtggtt aatcgttcat cttgataatc aactggcaag gagaatagtt ctttgatctt
    30841 ttctccgtat gacatggaag cttctgccct tggtccgatg gaattgttca tatttttagg
    30901 taatccaact acaaaccggt ctacttcata ttgtgctact aattccttca accgatcaaa
    30961 gccaaactct tcttctgctt cattgatccg aatgatctct actccttgag ctgtccagcc
    31021 aaaggggtca ctgatagcaa cacctactgt cttagaaccg acatccaagc ccattactct
    31081 cataaattca ctccatgatt tgagaggtag tccttgacaa ttacttccat gatctcatcg
    31141 cgttcgtgac ggcgaatcaa atttcgagca tcacgataac gaggaatgta ggccgggtct
    31201 ccagaaagca agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc
    31261 cgataaacaa tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa
    31321 cgtacggttt catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag
    31381 cttaccttct acaaccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa
    31441 acttccgaat aaattaagaa acactatttg tatgtttttt tccttttttc tgtagcttta
    31501 tcaaaagtac tcttttcata aagtatacgc tatatttctt tgaatgtatg tttttacaca
    31561 attttttcat aatattcata aaaaattcga atagctgcta ctaatttttt gtttttgttg
    31621 cttgtcctgt ctcataattg atcgtgtagc catcttgaac attgtagata aagacattga
    31681 attcaatttt tttgtcttca atgctttctg cttccaattg cactcccctg gctaccagtt
    31741 cattcccctc aaaatgagga gtgacacggt agcgtacatg atgatttgtt tctttgatat
    31801 atgccgcaat atcattttcg tgagcaagca tgtatggagc attcaacgaa cgtgtccctg
    31861 tcatcaagtt tttaatgttg ttattttgac cagtcagctg gaaaccaatt aaatggctgc
    31921 gattatataa ccattgcccg tcacttaatt tcttttgttt ccaacctgtc gggtcaatat
    31981 acaaaggctc tctcttttca gtagggaaca aatcttttcc aagcatagca ttggcttgtc
    32041 ctactctgtt caatttatca atatttgaat agctttccca tgttcctttg gaaaggctaa
    32101 gatcctcttt tgtaaattcc ggctgattcc cattgatttc tatttcttga tgttttccat
    32161 catattctgg cacatccgct acttgagaag cttgtggtgt ggaatcaaaa atagttgtaa
    32221 tctgctgaaa gatatctgaa tctttttgtg tggtggaaac accgaatgcc cctgcaatca
    32281 cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt
    32341 actaacagct ccctaaatct taacatatgt tcccctataa ataaatacta gaaagaaata
    32401 gaaacagcaa aaaataaaac ctgttttaag atggcttaaa aatacaaaaa agagacggat
    32461 tcctcgtctc taatcagtca ccactctatc tgtaaggtat ttattattat tcaaaaattt
    32521 tcttcatact caagacaata cactgatttc acaaccgctt ggtaaacggt gcgcaaaagc
    32581 ttgcatctgc ggtaataagt ccatccaagt taaaaacata agaagttgtt tttgcttggc
    32641 tgttcttatt acttgttgca gagcagcttt ttcacaacct attttaagcg ggtgacggga
    32701 atcgaacccg cgacatcagc ttgggaagct gggattttac cactaaacta cacccgcaat
    32761 acaaaaacag tatacgcctc ttttattcct tcctcaagtt tttttattct ttacgcttct
    32821 ggtatgtttt gtagtaaaat aaaactaatc aatcaattat tggagaagat gctatgacgg
    32881 gaaaagaatt agatctgttg ctggacactt gtttactcgc tgggaaaatc atgatggaaa
    32941 gcaatgcgga gatgtatcgg gtagaagata cgatgagtcg aattgcttta gcttctggaa
    33001 atttccgttt ggtcagctat gtcactcaaa ccggtctctt tattggtctt gatcgtacct
    33061 cgaccatccg aatggaacaa attaccaacc gttcgattaa tttagaaaaa gtagtaaaag
    33121 taaatgatct ttcacgtaaa tatgttgctt cagagctaac attagaagaa ctttactgcg
    33181 aactgcaagc aatcgaccga gaccgttctt tctttcctgt ctggttgcaa atcgttagcg
    33241 cggcagtgat cagtggaaca attatggtgt tatttggcgg aaagatgacc gatttacctg
    33301 tgactttagt cattggagga ttgggatacg cagcttatct atatagtttg aaatttttta
    33361 gaatcaaatt tttatcggaa tttttatctt ctttattgat tggcatagcc gctatctaca
    33421 gcgtacgttt tggcttagga agcaatcagg atctaatcat tatcggatgt gtgatgcctt
    33481 tagttcctgg agttcaaatt acgaacgcta tccgtgattt gctagctgga cattatgtct
    33541 caggagtttc tcgcggaaca gaagcgatga tgactgctac gatgatcggt tttgcgattg
    33601 cttttatttt ccagcttttt tattaggaaa ggaacacaag tatggggacg atatttattc
    33661 agttttcttt tagtttttta gcgactgcgg catttgccgt tattacaaat gtacctaggc
    33721 gttcattgat ttgttgtggt ctttctggtg cttttggctg gatgatctac tggatctgtg
    33781 ttcaaatagg cggatctgct gcttttggtt cattgcttgg ttctttagga gtagcattca
    33841 tcagcgatct tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat attcccggga
    33901 tggttcctct agtccctggt ggtttagctt atcaagcggt tcgaaatctc gttactgaat
    33961 cctatcgaga agcggctcat tatgcggtcc aagcaattat gattgctgga gcaatcgctt
    34021 taggtcttgt attatcagaa gtctttaacc ataacatccg taatttcaaa gtaaagcgtg
    34081 acgcaatcat gatgaaacga aaaaaacggc tttttaaata aacgaagtgt aacaaagaaa
    34141 aggttgtgaa agagacgttc agccttgagc gatgacaaaa tatctcacaa acattgcgac
    34201 aagtaaccgc aggagcaata agaagtaatt atgagtatta aaaaaagttt tgtaaaaata
    34261 gcttctcaca tttttacata aaattgactt aatactgaga aactgacttt tgaacaccct
    34321 ctatctgtaa aaaaggggct gcgacatgat ttttgtcacg gcccctcttc tttattcaaa
    34381 ttttttccaa aacgttcgtt gataataaac gatccataga gcggaaagta ttagaactaa
    34441 accgatcaca ggaatattca ctaaccaaaa tggataaaac aaagccaaca ttccgtataa
    34501 gacgataaga ataatactga tgatcaatga acccatcata gttgcgacta aacccaaact
    34561 tcctgaacct ctgttaaata attggttaat attggtccag ttcagcagca gcaaccgata
    34621 atctctagcg aaataacgta gtgacaataa gtagcagccg aataaagaac caaatagcaa
    34681 gctagggata aaaaacaaag gcagctgaaa agacagtccg cttaataatg cgatcccacc
    34741 agttaaaaaa ctctgcaata tccatccgaa tctgaacttt tcttttaaat attggttcat
    34801 agagattggc aaagaacgga taaacagaaa attttcttga tccaaagaaa taagattgct
    34861 gataaaagaa gtctgattaa ctgttaagaa agccaatgcg atccctacca ggaaaacaac
    34921 accgattaac ttattatcta aatcagttaa ttttatttcg cctcctaggg caaaagcaag
    34981 aataaaaatg attggagtta acaaggaaga agaaagaact tgcataatca aattaggttc
    35041 ttttactaat tgagcattgt aactaaataa aagctgatgc acattttggt tctttttatg
    35101 ggttcttttt attttgaact cactgtttga atcagtcagt tgctctgtaa gctttggtac
    35161 tatcagctgt tttacagcaa ggaaaaatcc cagagccgtg atagctagta ttccccaacc
    35221 caataaacct tgttgagaaa gtggctgact ggcagcatca tagaatggca ataatatcga
    35281 aatcggatgc cgatctgtta actcattttc aatagtcccc gtttgatgat tcatccacat
    35341 gattccagca aatgcgatca gcatcgagct gccaagcaag atactagaaa ctgtttttct
    35401 atgtttttta aacactgctg ttcgagccaa accaaaaaca atcaaactag ccagacaaaa
    35461 aagaataatc aaaaatagag aaaacaaaag gattgagata atgatagtaa gcaaaatccc
    35521 tagatcagct cgccatccgg ttaaaataaa catgactaga agcggaaaaa caaaaggaat
    35581 gatggtcaaa gcaactacca aaatctttgc tgaaaaaacc tgttcttgtc caaaaggcag
    35641 aggcaaaaat acttgcaggt catttccttc aaaaaagata ttataaatga ctgatatact
    35701 ttgagttaaa cctaacatac caaaaagtgc cacgtaataa gtaaagaaac ctggcaattt
    35761 aacaaagttc atagcaaaca tagcaaatcc gtaaatcagt aaaaaaatca gccctgaaaa
    35821 aagattttga gacaaaagat agatagttaa tcgctttccc ttttttcctt tctctcttgc
    35881 tttattcgtg agctgtggat taacatataa aagattaact cgaattaatt ctaataattg
    35941 tcgcttatac atacggtcca tctcctgtcg cttgccttcc agccattttc aaataaatct
    36001 tttctagcgt ttcatcaggt gagccattca ataattcact aactgccccc tgataaatca
    36061 attcaccttt cttcaaaata gccagtttat cgcataactg ttgtgcggta tccaatacat
    36121 gtgtagaaaa aatgactgtt tttccttttg cggcatgagc cttcatcatt tctttcaaat
    36181 caaatgctgc ttgaggatca agtccttgca aaggttcatc caatacccaa atatctggat
    36241 ctggcagcaa cgtgccgatc aaaatcgttt tttgacgcat tccatgtgaa aaacttgcga
    36301 ttgtttcatt ttgatgacta tacatatcaa aaagcgctgt caattctgct aaacgctttt
    36361 cttttttttg tgtgtctaat tcataggcag ctgaaatcaa gtcccaatat tctccagccg
    36421 tcaattgtaa aaaaatatct ggggaatcag gcacataacc gattttttgt ttgatactca
    36481 atcgattttc cgtcaatttc attccatcta ctaaaatcgt tcccgatgat ggttcaataa
    36541 tactaactaa acttttaatc gtagttgatt ttccggcacc attatgtcct aaaaatccaa
    36601 aaatttcccc ttgtcttatc gagagagaca agtcttttaa tgcttgtttt ttcccgtaac
    36661 ttttacttac attgatgaat tcaatcattt ctgtactcct tttgtttgtt attacttttt
    36721 attgtaccct tcgttattgg tggagtaaat gtcaaaaaga gtgcataaca aaaaaggata
    36781 gaaacgacta ggctgctact tgggttgttc tcttctacag gaaaacactc ttctaacagg
    36841 taagatatga gtctttgttt tctttaaaat gattcatttt ctatcttttc ttgcataaaa
    36901 ttctttagtt tatcaatact tctctttccc atctctagtt gttctccatt tgcaaatgtt
    36961 actatttcgt ttactctgct aatagatgta atgttttcag tattaatgat ataagctttt
    37021 aaatctttac ataaatactc atattcgagt gattttttat attttttaat tgtgccgttt
    37081 acgagaatct cttcatcttt caatactagg cctattttat ttttataacc tggcatgctc
    37141 attagataaa ggatttcatc gtaatggata tagtgatcgc tattaccata acggacagtt
    37201 gtccaccttt ttttcgtagc atgttgaaca ggatagtttt ctttttcttt caataagtaa
    37261 ctttggatgc tttttttatt tttttcataa gtgaattctt ttatcaaata accactaggc
    37321 gtaatctttc gattaataac agttattgct ttattcggat gagacgttag ataaacaatt
    37381 ttacactttt cattcttttc tctaagcatt tcgcctagtt cgataccgct ataataggcg
    37441 ttcaaatcga tgtctaggaa aaaaatatct gaggaataga tttcattatt tttaatgaat
    37501 tcaacaaatt gtataacgtt caatccctga acgatctcaa atttccaagg acttaataaa
    37561 attctggatt cttccaaaat ttctactagc attttttcaa aaaccaaact atcatccaaa
    37621 ataaatatac gcgttaccat aaaaagtacc tccactcgat attttactat caaaatcatt
    37681 tcatacaact tttcaacata catgttagga ataaattgta attctcttca caatcttttc
    37741 caacaccttt gaaaccgata tcaaatactt tcaggatgct gcttctaaaa taaatatcaa
    37801 acaaatgaat acatcatgca aaaaaagcca gacaaccgac gtccttgcgt gtcagctgtc
    37861 tgagctatgt ttatttattt tgttctttta aaaagaacga tacacatact gatttttcgg
    37921 agcagagact ttctcagcgt ttgtaacaac tacagatggg atttcacgct tgaatggttg
    37981 ataatagtct gattggatgg ttccttctat tttataccat tcatcatttt taaccgttac
    38041 tccttccggc atatgcgtca acaaaccaaa gactcctgaa tctgccacac aatggataat
    38101 gccgaatcgg aacaagaaga tatctgtatt gctatcgttc ggtgtttggt aaacaaatcc
    38161 ttcatagctg atttttttgc caatgaattc gctagggtaa ttataaatta gctccataac
    38221 ttctaaatag ttttcatctg tgatcttgat agtttgctta tccccatact tttctttcaa
    38281 ctcattcatt tggtctaagt agtctgattt gttgaaataa atactggtat ccggctttaa
    38341 gtattgtgtc tgcatctccg gatcccctac tgattcttta cttactggga aattaaatcc
    38401 tttggcttct acgatcgtag tgtcgagact aactgttgga aacaacgtcc ccacgattac
    38461 tggcaaagct agcagtacat aagcgattcc acgttgataa ggttttgaca acccatggtc
    38521 atgatcgtgc tcgtgtgcct tttcttctgc ttttccgttt ttgacccaca aatacagctg
    38581 aactaaagcc aaaagagcag ataaaatcat tgaaagaata gctaagtagc gataatggac
    38641 attgatatat tgatcgagtt tgccagaaac ttgcaagtac atcatcaatt cgaaataccc
    38701 agataaaatc aaaaaccgca gcatattttc cctcctaaat aaataatgtg aatccaataa
    38761 cgctaagggc aatcagtccg atcagcgata aaataaagcg tcctgtaaaa tatcgtttca
    38821 tcatcagtag atttttgata tcgaccattg ggccaaagac caaaaaggca gctacaggtg
    38881 ctgtgccgaa cagacttaac aaagaagatc ctataaacgc atctgcttct gagcaaagag
    38941 acatcgtaaa ggccaataac agcatgatga taatggctaa aatctttgtt gagccgagcg
    39001 ttagcataat tctagttggt aaataagtct gcatcgcagc tgcgattaat ccgccaatga
    39061 tcaaataacg acctgtatca aaaaattcgt cgattgaatg tgtcaacaca tgcccagttt
    39121 gatggatcag ctgatttttt ggttcattct tctcactttg ttcttcttca tggacatgat
    39181 gatggtgatt ttctgctact ttagacgtga ttttttcttt caatacagcg tctttttgaa
    39241 aataggcaag ccagacacca atcacaaggg caacaaaaaa gctgcctatc acccgccagc
    39301 ttgccatctg ccaagaattg ccaaatgcga taaaagtgga aaaaatcacg attggattga
    39361 tgatcggagc agtcagcata aaagcaaacg ctgtgtgaac tggtacgcct tttttaacaa
    39421 attgatgaac gatcggtacg atgccgcatt cacatgaagg aaagaaaaat cctaagatcg
    39481 aaccagtaaa aatagagaag atacgatttt caggaagcca tctcttcaca cgttctgggg
    39541 tcaaaaatac ttgtaaggcg cctgagataa gacagcctaa taatacgaaa ggtaatgctt
    39601 caataacgat cgataagaaa atcgtactca tctgcaaaac cgaatttgga ataaaagaaa
    39661 acatgagttt cctccttttt atttctagtt cattcgcttt gcaaatatac gcttttattc
    39721 aaaaaaaaca agttgctctg taaattttta cacaactctt attttttacc aaagcacttc
    39781 aattgacttt ttctgttatt cccattaaat taaggtctca tacatatcac ttctatcata
    39841 aaagggagaa taaaaatgaa acaaaccact aacttgactg aagttcaaga catactgcaa
    39901 cagtcatccg ttgccgtcat ttatctttcg atgcctaatt gcagtgtttg tcacgctgtt
    39961 cgtccacgac tagaggaact tctcacacat tatgatattc cggcacttca tttggatgca
    40021 tttgaaacac cagaagtagc tagtaaattt gaagtgctga ctgcgccagt tgtcctgatt
    40081 tttcatcaag gaaaagaagt ctttcggcaa gcacggttca tcgactttaa aaaaatccgt
    40141 tatctactag atgaactgac agcagaagat gattcccttt cttatgaaga aatcttccga
    40201 acaaaataat cgttgtccaa acagaaaaaa gcgattgaac aagtgaaagt tttgccttca
    40261 tttgttcaat cgctttttat ttttattctg ctgtattttc ggaatgcgca tttgcgtcag
    40321 aatgcaagaa atagatcaag gtttgcaatt cattcgtcaa atcaacattt tggatcaata
    40381 catctgacgg agcattcaat cgagcagcgg taaagttcaa gattcctttg attcctgctt
    40441 ccgttaactg attgactact tcttgtgctc tttcagcggg tatcgttaaa attgctactt
    40501 cgatttgttg gatcttgatt tgttctgaca tatcttccat tggataaacg gggataccgt
    40561 ctacgatgcg cccaacgatt tcctcattca catcaaaagc acaactgata cgtatgctat
    40621 tactttgatg gaatttgtat tttagtaaag cacttcccaa gttccctaca ccgatcagcg
    40681 caacatttgt caattgatcg tcgtttaatg tctttgcaaa aaagtgcata agatcttcta
    40741 catcataacc atacccacgt tttcctaatt cgccaaaata agaaaaatct cgacggatcg
    40801 ttgcactatc tacttgaacg gcttcactta attcagttga agacactttt ttctttccag
    40861 cattatttaa aatccttaaa tagcgataat acaaaggcag tcttttagct gtcgcctttg
    40921 gaataatatg atctttcacg aaatgacctc cacgtattgt ctaattacat ctttgatttc
    40981 tccatcatag cagtatttat aggaaaaaat caattccatt gctcagatat tgtgaaattt
    41041 tattctttat tcatttattt atcatttctg caaatctcgc agtataatgg aatttatggt
    41101 agactactaa ttgaaaaata gttttttatt tgaggagaat tcacaaagta gaaaagagga
    41161 ttccatgata ttattacaag ccaatcagat tgctcgccat tttggtgcag aagtcttatt
    41221 tgaaaacata catttagaaa tcagtacagg cgctcgtgtc gctttagttg gcagaaatgg
    41281 cgctggaaaa tcgactttat taaagatcat tgctggaatt gaagcacctg atgaaggaaa
    41341 gatcgctaaa aataaaacag cgactctcgg atacctcgct caggacacag gacttgattc
    41401 caatgaaaca gtttggaacg agatgctgaa agcttttgaa caagttagac aaatggaaga
    41461 gcggatgcgc gagctagaga ttttcattag cgaaggtacg ccagacacac ctgcttatag
    41521 ttcttattta aaagaatacg accggctgca acacgatttt tcagatcgaa acggctatgg
    41581 ttatgaaaat gaaattcgtt ccgtattgca tgggtttaaa ttcgattctt cattttatga
    41641 tcagaaaatc gatactttat caggcggaca gcgcacacgt ttagctttag ctcgcatgct
    41701 tttgcaacac cctgacattt tgattctaga tgaaccaacg aaccatttag atatcgagac
    41761 actttcctgg cttgagaatt atctgcaaag ctatactggt gcattattga ttgtttccca
    41821 tgaccgttat tttttagata aagtagtcaa tgaagtctat gaattaagca gaagaaaaat
    41881 gcgccattac aaaggtaact attcgaaata tctagatttg aaagcagaac agttagcaag
    41941 tgaatggaag gcttatgaaa agcagcaaac agaaatccat aaattagaag acttcgttgc
    42001 tcggaacctt gttcgcgcct ctaccacaaa acgagcgcaa agccgcagaa agacattaga
    42061 aaaaatggac cggctggatc gtcctcaagg ggatgaaaaa caagcaaact ttctattcag
    42121 tatagaaaag ccttctggaa atgtagtctt gcaaatcgaa gatgccgcta tcggatatga
    42181 taatgtcacc ttatctgaac caatcaatct agatatccgt cgtcaagaag cgattgctct
    42241 agtaggtccg aatggtatcg ggaaatcgac gttgctgaaa tcaatcattc accagatccc
    42301 ttttatccgt ggaaaagaaa cgctagggac aaatgtcacc atcggctatt acgatcaagg
    42361 tcaagctgat ctgcatacaa acaagacgat cctagcagaa ttgtgggatg agcatcctac
    42421 cactccagaa aaagagatcc gaagtgcttt aggtgctttc ttattttctg gcgaagatgt
    42481 agaaaaacca atccctctgc taagtggcgg tgaaaaagca cgggttgctt tagccaagtt
    42541 agcgatgaac aaagaaaatt ttttgatttt ggatgaacca acaaaccatt tagatattga
    42601 caacaaagaa gtattagaaa atgcattgat cgattatgac ggcacactgc tttttgtttc
    42661 ccatgaccga tatttcatca atcggatcgc gacaaaagtc gtcgaactct ctgaaaatgg
    42721 cagcaaagtc tatctaggag actatgatta ttatttagag aaaaagcagg aagaagaaga
    42781 aatcgccgaa ttgttggcac aagatgagcc agaatctgct cctgctccca cgaacaaatc
    42841 tgattattac caaagcaaag aacagcaaaa gcttatccgt agccttcaac gaaaaattac
    42901 tcaggttgaa gaagaaatgg cgcggatcga tgaatggatc gatcagttgg aacaagaaat
    42961 ggtcgcgcca gaaaacctgg aagatcacgt aaaactcaat gagctgaacc aagatttaga
    43021 agctgctaga caagatcaag aaacaaaatt gacggaatgg gaagaactaa gcttggaatt
    43081 agaagaatat gaaaattaac gaaaaaacca aaacagaaac cattcatgct gttttggttt
    43141 ttcttttttt attttatcaa acaaaaattc catatttacc tttttcacca ataagaagac
    43201 caattactag tcattctcac taaagcttct aaataatagt aatcacccca aatacaaggt
    43261 gtatttaccc cattattttt gggcttatta taaacagact gtgttaaaaa gccatcttct
    43321 tcttccgttg tagcatatct ttctttcaat tgtcgcataa ttttccaaac agcagactgg
    43381 tataagttct tttctgtttt gtctaataga cctgattcta ttaattctaa taatccacaa
    43441 accatgatca ccatactaga tgtatctttt tcttctctgc tagaatcatc aaagatcaaa
    43501 tcccaataac aaatggtatc ttctggcaac cgattcataa aataattggc taatattttg
    43561 cttaaatcaa aatatttttg ttctttcaaa taacgataac ttaatgcaaa accgtaaatc
    43621 ccccatgctt ggcctctagc ccaacaggaa ttatcactat aaccttgatg cgtacttcca
    43681 tatcttggtt ttccatcttt gacatccata taaaatgtat gataagttga accatcttct
    43741 cttactaaat aatctgctgc attttgaata tgagcctgag cagctttttt atatttttga
    43801 tttccagttt cttcactcgc ccaatacaat aatggaagat tcatgcaaca atccatgatc
    43861 attctacctt gttgtgaagg atcactagga tctccccaag cttgtataac tcctgccttc
    43921 tcccaaaaac gctgaagtaa tgtttctgca gcatcaattg aagcttcctt catttttcga
    43981 tttcccgtta ttttataatc tgctacggtt gataaagaat acaaaaaacc aagatcgtga
    44041 tgattagtga taatttgaca gtcaatcctg tggataaaat ctatatctgc tttttcagct
    44101 gtttgttgaa ataaaggatc acccgtcttc tcaaataaca accaattaat tcctgtccaa
    44161 aatccttctg tccaaccaat attgtctctt aatggataac gtccttttat aggacactcg
    44221 cttgggaatt gatactgact gttactgata cgtttgatta acctctccgt gtgtctgcga
    44281 caacgctcga tctcattatt ccattctttt tccattaatt ctttataaca ttcatttatc
    44341 catttttctg gatgtattat ctcttcaaac atcactttat caagcatttc cccaccagcc
    44401 tttagcataa ttttagtctt tttttattgt ttttattttg gatagttagc ccggttgtcc
    44461 catcaaaatc aattttggga cgattgttcc atacttctaa actataatcc cgatcttgat
    44521 gagcaaccac tcctgttccg agccaatggg ttccttttcc aaatgtaccc tctaatccag
    44581 gaataaaagt gttttcccat gtcatcaagt ttgtattggg caaacccata atagctgtgg
    44641 attttcgttt aatagtatcg tcacttaaac agataatacc tgaaaagcca gcttcactca
    44701 ccctatagtt ttccttgtct tcttctatga ttattccttc tttctcagga gaacattcaa
    44761 tagcaaaccc tgtctcatat agaacatatt ctgtatctaa ttctactttg tgtacgcgaa
    44821 tctgccagcc ttcaaaagga ataagccaag ttctgatttt tgcctgtaaa gtaggttccc
    44881 atgtagaggc aagtacctct ttcttacata caatgtctcg tacattattt cgaacgatga
    44941 actgtccata ggttttgatg ctctcattct taaaactggt cgtgatatat gtgtccgctc
    45001 gctgaatccc tagcattgaa tccattgcca tttctgcttt caattgattc gctcgcggta
    45061 tcgaaaaacc aaattgacta gaataagcaa attttaaata tttttcattg gtcaatgtat
    45121 ggaaatttgg ccctaattgt cccgcattca aaaaaaatgt atgtccatta tcatgttgta
    45181 tgagcaaatt ggcttctgaa agaagtttag ttccttcacg aataggcata ggcatttctt
    45241 tagccgtcca atactcatgg tcttcaggta gcgccaacaa taaaaaaatc ttattcagcc
    45301 ataatggaga taacgtacta ttatagggtt cagtcaatgt caattgaggg taggtataac
    45361 caatattcag gatacctttt tcatcaaata tttctttttg catccaccat cttatattcc
    45421 gattgactaa ccccttgatt tcacctaatt taaaaggcaa aagatcggcc cacaacatag
    45481 ctgaccaaaa gcttgaaact gcaaaacgat aaatcatgct tcgaccaaaa ggcacatttg
    45541 caccatcttc cgcaaaaaaa tgaatatagt ctctagaaaa atcaatagct cggttcttat
    45601 atatctggct aatgggcttt tctggttcta gtacgctata aatcaaacca taataatgaa
    45661 atgcaaaagg attataataa tcttggcgac ctcttgaact gtctcgatac cagccatttt
    45721 gaagataaca attttctata ttatcaaatg cttctttcag tgctgattct ttaactggat
    45781 aaccaaattt ttggataatt gttccgacaa ttatcttaaa aaattgccaa ttaccatctg
    45841 cacattcata ttcaaaaatt tgcgagaacc aatgaataat ctggcttttt tctcctgtat
    45901 ctaatttttt ccataataat tgttcatgat gatataacgc taataacatt gcgggcattt
    45961 ctaccatacg ctggtctctt tcatgtattt ctccccaata atcgggatgg gaaggatctg
    46021 ttccagcttt tattcctgat agaattaatt tcaaccatcg ttcatctaat tggtcaattg
    46081 cgggccccgc tccccataaa acccgagaga atccttccat tcctgcaatt ttatttccat
    46141 atcctacccc ttgatgtata taatcaattt gtgcgccact tgctcgataa aacggctgga
    46201 gtggttgatt tatcttttta aataagtcaa agtaatcctg tctggtcctt aatagtggta
    46261 ctggatgttt cattgtattt tctcctcttt ttactaccaa atagctaatt ccgactgttc
    46321 taatcgaagt aagccttcaa taaaaaaata atcagaatag atcagtggca cattataagt
    46381 cagttctcta tggtatgctt ctgctccgcc tccaatgatc ccatcttcat ccatattcca
    46441 atttgcatag tgcttttctg tcttttggag tatattgata gcagcttcat gatacatttt
    46501 tgatcgtaca gaggaaacat atcctgctaa ttccagaaga ccgcatgcag cgcatactcc
    46561 agcactggta tcaatattcg aatcttttac agcaaaatca atgagaggaa tataaccagt
    46621 tttttcgatt ttctgaataa aatttactgc tatcttttct gctatagcta gatagtacgg
    46681 attttttgtg taacgataac ttaacgcgaa tccatataaa gcccatgcat ttcctctgct
    46741 ccaacaagaa ttctcattgt atccttgccc gccaagtaac cgaatatatt tccctgattc
    46801 tgcatcaaat tctgcgatat gcccggttga tccatctttc ctcactagat acttagctgt
    46861 tgtatctgca tgctgccgag caattttaga gaacctcgga tcaccagttt ccctcgaagc
    46921 ccaatacagt aatggtaaat tcatcatact atcaataatc atccaaccag gtttatggtc
    46981 attccatgct actaaatagt tccctgaaat attatagcgt ccagcaagta ccgtagctgc
    47041 atgtaaccct agttgtcgac tatgtttgtt ttgagactgt tgataatgtg caatagccga
    47101 tggtaagaat aaaaaaccta catcatgatg aacgtctgaa aaatggtaca gggcagattc
    47161 taataattgt tcttgttgta ttgctgcctc tttgaacttt tctcgttttg tcttgtgaaa
    47221 gagctgccat aaaataccac tccaaaaacc attggtccac cagtcgattc ctctgtcctt
    47281 tattacgtct gtatattttc cgttgtaaga aatatatgga attttattcc ccattctgtc
    47341 taattccgca tctatctttt cagaagctac attcaaaatt tttcttgacc attccttcgt
    47401 ctgttcattc atggatatcc cgccattctt ttaattttta tgaatgtgga gttgctccta
    47461 gaacgaacta gatggttaac attcgttatt ttttttactt atttgcagct ccaatgtcat
    47521 tctccttaaa tttagactgc gcccacacat aaatcaagac cattgggatc actgaagttg
    47581 ctaaaattag cataattgcg ccataattaa ttggctcacc tgtactagaa accgtcatat
    47641 ttttgataat taagggaacc gtatatttat ctttatctga aagcatgact aatggaaata
    47701 gatattgatt ccaacttccc ataaagataa gtaaaccact agtaataatc gtaggtgata
    47761 aaatcggtac agcgattttc gtaaaaatca cccactcatt ggcaccatcg attctggcag
    47821 cctccatcag tgctgtagga aattgactgc tatattgtcg catcataaac actgaagttg
    47881 tactggcaat cgaagggatg atcacacctg cataagtatt cgataatcct aaacgactca
    47941 taatgtaaaa taaaggaatc aatgtggtaa aaaaaggcac catcctagat agcataactg
    48001 tattgaacaa cagtttattc cccataaaac gatactttgc taatgcataa cccgccattg
    48061 tagaactggc tgtagctaaa actgtactga ttacagcaat aacaatcgaa ttccaaataa
    48121 tcaagggata attatatctc gccatgatgg cttgccagtt ttttaatagc atatccccaa
    48181 acttgaaatt gattccgcca cttaaaattt cttgattact ttgcgttgaa gctacaatca
    48241 tatataaata tggcatcaaa aatattaagg caccaaacac aataatgacg tacgtaaaaa
    48301 ttctgctttg tatcttttgt ttagtcattt ttatcacctc cgaatttaaa ttgaatagcc
    48361 gataatataa cagatacaaa caccatcgtc caggcaatag cagaggcttt tccaaaatca
    48421 accatttcaa aacttgtttt atataaattg actcctacga ccaaagctga attttctggt
    48481 ccattggaag tcccaaagaa tatatttggg atattgaaat tacccaagcc attgatagtg
    48541 gctaaaactg tggccattaa aatgattggc tttagcatgg ggatcgttaa taaaaagaat
    48601 tgccgaaatt tcgatgctcc atctatttca gcactttcgt atatctcttt agaaatccct
    48661 tgaagaccag ctaaaaataa aatagtgtta tatcctacac taagccaaat catcgtgatg
    48721 atcattgata ttcgtatcca aaaaggattt gttaaccact gtacaccgtt aattcctagc
    48781 gcattcaaaa atccattgaa taacccagtc ggattgaaaa aagtcatgaa tacggaagct
    48841 acagccaccg ttgatgtaat ggttggaata taatagacag ttctaaaaaa tccttttcct
    48901 tttaactgtg ctgaattcaa taaaacagca aagtaaatag ctaatagcat cgttattgga
    48961 attgatcctg ccatgataat caaaacattc gtgtaagatt taataaacaa tggatctcgg
    49021 aagatagcta gataattgtc aaatccaaca aattcaaatg agtagccaat tttcataaaa
    49081 ctaaaagcaa acgagataat gatgggaatc aaaatcgtca caataaagaa aataacaaaa
    49141 ggtgtgacca atccataagc acttttttta tgagacgtat ctgttgcttt tttcttgctg
    49201 ttctttattg tcttgcttgg taacgaagat tgcatcatcg ttaagtcctc ctaatatttt
    49261 ttaatcacca gttaatttta cgccgtactt atgagcaaaa ttttctgctt cttttagata
    49321 tgaattttca aaatcacctg tttgccaata gttatatgca gcggtagtca aataagacgt
    49381 agcatctgaa taagtggctg gtaaaacagt atttgatgta ttttcattga ttttattcaa
    49441 tagttcatag tatttttgtc ctccttagta gtcaaacttt ttctcagctg cttctgtttg
    49501 ataggccaat gagttagatg tagccacgcc cagctccagt gcgttggcta agcattcttc
    49561 atctgtcagc ataaatgtaa gaaattgttg tgccacttgt ggatttttgg catctttagg
    49621 gacataaaaa cttgatcctc ctgatactgc accttttcct ggattttctt ttgaaaaagg
    49681 aatcagtgga gccatgcgcc atttgtcaga tgcatcagga aaattcaaag acatattcgt
    49741 agcataccag ccgccagcaa caaacattgc agattcttga aatgctttct cagcatcttg
    49801 tgcaccgtag aaatgtacaa tatctgagtc gatcatttct ttgattaact ttgcagcatg
    49861 tttcgcttct ttagtattca gacggatatt tccttcttca tcaagcaaag attgattctg
    49921 ctgggctagc atgttgataa acaattcttg gtccccagat tctggcaagg caattccaaa
    49981 actaccagtt ttttctttta atgcttttgc cacctggata tattcttccc aagaactgat
    50041 ttcttcaaaa tctacaccta tttcagcaaa tttttttggt tgataataac aaagtgctgg
    50101 ggtaaaatca ctagcccaag gaataatcgt ttcattactc gtttgttgac gcaaaatatt
    50161 caatttttga tcataaaact gatcgccata tttgtcataa aatccatatt catcagcact
    50221 ataaaaagca tcttgaaatt tttccagcac acctgctgct gaagtgtctg ccatggagat
    50281 aatatccggc atatcttcgt gtgttaccaa ttttggggtg atcatttgta ctgttgctgt
    50341 tcccgaagct acattcacaa tttttaagtc aatatttgct ccagttttgc ggttatagat
    50401 ttttgcagca ttttcaacat agtttgtcag tttgccgttt gcgacatata cttctaatgt
    50461 agtggctttt tttatcattg gatctgcgat gtttgctgtt acttcttcgt tagtttttcc
    50521 acatgcactt aagctgaata atagggtaat gtttagcaaa gctatcacca atttgtatct
    50581 ttttttcatg tctatccctt cttt
//
LOCUS       DARBEG010000012        49362 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000012 DARBEG010000000
VERSION     DARBEG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.012"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            71..1129
                     /locus_tag="SCK20_000681"
     CDS             71..1129
                     /locus_tag="SCK20_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG1144769.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1133..2167
                     /locus_tag="SCK20_000682"
     CDS             1133..2167
                     /locus_tag="SCK20_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG1144770.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2269..2985)
                     /locus_tag="SCK20_000683"
     CDS             complement(2269..2985)
                     /locus_tag="SCK20_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1144771.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2954..3739)
                     /locus_tag="SCK20_000684"
     CDS             complement(2954..3739)
                     /locus_tag="SCK20_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG1144772.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3730..4479)
                     /locus_tag="SCK20_000685"
     CDS             complement(3730..4479)
                     /locus_tag="SCK20_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1144773.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4777..5301
                     /locus_tag="SCK20_000686"
     CDS             4777..5301
                     /locus_tag="SCK20_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG1144774.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5317..6816
                     /locus_tag="SCK20_000687"
     CDS             5317..6816
                     /locus_tag="SCK20_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG1144775.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6904..7506)
                     /locus_tag="SCK20_000688"
     CDS             complement(6904..7506)
                     /locus_tag="SCK20_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1144776.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7610..8566
                     /locus_tag="SCK20_000689"
     CDS             7610..8566
                     /locus_tag="SCK20_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG1144777.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8871..9827)
                     /locus_tag="SCK20_000690"
     CDS             complement(8871..9827)
                     /locus_tag="SCK20_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1144778.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10001..10561
                     /locus_tag="SCK20_000691"
     CDS             10001..10561
                     /locus_tag="SCK20_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144779.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10554..11378
                     /locus_tag="SCK20_000692"
     CDS             10554..11378
                     /locus_tag="SCK20_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG1144780.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11388..12005
                     /locus_tag="SCK20_000693"
     CDS             11388..12005
                     /locus_tag="SCK20_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1144781.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12023..12706
                     /locus_tag="SCK20_000694"
     CDS             12023..12706
                     /locus_tag="SCK20_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG1144782.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13389..14624
                     /locus_tag="SCK20_000695"
                     /pseudo
     CDS             13389..14624
                     /locus_tag="SCK20_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            14632..15519
                     /locus_tag="SCK20_000696"
     CDS             14632..15519
                     /locus_tag="SCK20_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1144783.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15536..16372
                     /locus_tag="SCK20_000697"
     CDS             15536..16372
                     /locus_tag="SCK20_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1144784.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16375..16998
                     /locus_tag="SCK20_000698"
     CDS             16375..16998
                     /locus_tag="SCK20_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144785.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16995..18287
                     /locus_tag="SCK20_000699"
     CDS             16995..18287
                     /locus_tag="SCK20_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1144786.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18646..18743
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18859..19440
                     /locus_tag="SCK20_000700"
     CDS             18859..19440
                     /locus_tag="SCK20_000700"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG1144787.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19443..20750
                     /locus_tag="SCK20_000701"
     CDS             19443..20750
                     /locus_tag="SCK20_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG1144788.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21043..21531
                     /gene="purE"
                     /locus_tag="SCK20_000702"
     CDS             21043..21531
                     /gene="purE"
                     /locus_tag="SCK20_000702"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG1144789.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21524..22648
                     /gene="purK"
                     /locus_tag="SCK20_000703"
     CDS             21524..22648
                     /gene="purK"
                     /locus_tag="SCK20_000703"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG1144790.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22661..23956
                     /gene="purB"
                     /locus_tag="SCK20_000704"
     CDS             22661..23956
                     /gene="purB"
                     /locus_tag="SCK20_000704"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG1144791.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24136..24513)
                     /locus_tag="SCK20_000705"
     CDS             complement(24136..24513)
                     /locus_tag="SCK20_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144792.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24769..25497
                     /locus_tag="SCK20_000706"
     CDS             24769..25497
                     /locus_tag="SCK20_000706"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG1144793.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25497..25751
                     /gene="purS"
                     /locus_tag="SCK20_000707"
     CDS             25497..25751
                     /gene="purS"
                     /locus_tag="SCK20_000707"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG1144794.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25753..26424
                     /gene="purQ"
                     /locus_tag="SCK20_000708"
     CDS             25753..26424
                     /gene="purQ"
                     /locus_tag="SCK20_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG1144795.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26425..28647
                     /gene="purL"
                     /locus_tag="SCK20_000709"
     CDS             26425..28647
                     /gene="purL"
                     /locus_tag="SCK20_000709"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG1144796.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28632..30071
                     /locus_tag="SCK20_000710"
     CDS             28632..30071
                     /locus_tag="SCK20_000710"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG1144797.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30094..31146
                     /locus_tag="SCK20_000711"
     CDS             30094..31146
                     /locus_tag="SCK20_000711"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG1144798.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31143..31721
                     /gene="purN"
                     /locus_tag="SCK20_000712"
     CDS             31143..31721
                     /gene="purN"
                     /locus_tag="SCK20_000712"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG1144799.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31718..33253
                     /gene="purH"
                     /locus_tag="SCK20_000713"
     CDS             31718..33253
                     /gene="purH"
                     /locus_tag="SCK20_000713"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG1144800.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33269..34543
                     /gene="purD"
                     /locus_tag="SCK20_000714"
     CDS             33269..34543
                     /gene="purD"
                     /locus_tag="SCK20_000714"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG1144801.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34665..35579
                     /locus_tag="SCK20_000715"
     CDS             34665..35579
                     /locus_tag="SCK20_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1144802.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35712..37277)
                     /locus_tag="SCK20_000716"
     CDS             complement(35712..37277)
                     /locus_tag="SCK20_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1144803.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37270..38721)
                     /locus_tag="SCK20_000717"
     CDS             complement(37270..38721)
                     /locus_tag="SCK20_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1144804.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38735..39751)
                     /locus_tag="SCK20_000718"
     CDS             complement(38735..39751)
                     /locus_tag="SCK20_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1144805.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39971..41059
                     /locus_tag="SCK20_000719"
     CDS             39971..41059
                     /locus_tag="SCK20_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG1144806.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41113..42639
                     /locus_tag="SCK20_000720"
     CDS             41113..42639
                     /locus_tag="SCK20_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG1144807.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42641..43846
                     /locus_tag="SCK20_000721"
     CDS             42641..43846
                     /locus_tag="SCK20_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1144808.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44103..45080
                     /gene="guaC"
                     /locus_tag="SCK20_000722"
     CDS             44103..45080
                     /gene="guaC"
                     /locus_tag="SCK20_000722"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG1144809.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            45344..46768
                     /locus_tag="SCK20_000723"
     CDS             45344..46768
                     /locus_tag="SCK20_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1144810.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            47229..48338
                     /gene="pdhA"
                     /locus_tag="SCK20_000724"
     CDS             47229..48338
                     /gene="pdhA"
                     /locus_tag="SCK20_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG1144811.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            48341..49318
                     /locus_tag="SCK20_000725"
     CDS             48341..49318
                     /locus_tag="SCK20_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG1144812.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        15738 a         8505 c        10864 g        14255 t
ORIGIN      
        1 tgttaatacg tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag
       61 gaggagaaag atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt
      121 cagtgcaatc acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg
      181 gattttttct gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc
      241 agcactggca tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat
      301 caatgtcttg atttggggat tagttattta tattattctt attctagttt caaaaccttt
      361 tttcttaagc tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc
      421 tacgaccttt ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta
      481 ttcctcaatt ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat
      541 gagtggcttg aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat
      601 cgctactttt gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt
      661 tacaggtgca gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc
      721 gtggctattt cagccgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc
      781 aatcggcgca acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg
      841 gtattacagt ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt
      901 tttgacggca acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat
      961 gatggctaca ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc
     1021 acttttagcc tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt
     1081 atggatcgtt gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga
     1141 agaaagtatg agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa
     1201 attgaatatt taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat
     1261 cgtgtcagca aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac
     1321 caatcttgca aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt
     1381 caatcatatc gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc
     1441 gtattcgcca aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa
     1501 tactgcgtat acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt
     1561 tagtcgtgaa atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac
     1621 ggaagctgtt ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac
     1681 agaagctgct aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga
     1741 tattgtcagt attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg
     1801 atttttagct caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca
     1861 agataatgaa gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga
     1921 tgtttttgca aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc
     1981 agaccctgct atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt
     2041 aggttcaaat acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg
     2101 tgaaataata gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt
     2161 aagataagaa aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt
     2221 tgttacttct gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc
     2281 tgtgagcttt ttccgcccat tctttgtcaa ttcataacag gttagcggct ggccgatttc
     2341 caaatatttg attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt
     2401 gacggatata cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc
     2461 ttcttttaga ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa
     2521 atcgatgaat gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa
     2581 tttctgatcg gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat
     2641 cgccagctga aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg
     2701 gatcacattc tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc
     2761 atttccttgc tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac
     2821 atcaagcagc agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt
     2881 tctgaaactt tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc
     2941 tgcaaccatt ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt
     3001 ttggtaattc tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc
     3061 caccgtggcg tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct
     3121 tcgtcgaaaa acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgc
     3181 catcgcagac ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga
     3241 cctgtccgtt tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc
     3301 ctaacacttg caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat
     3361 cgctttctaa cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa
     3421 aaccggcaat tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat
     3481 tttgatttag ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac
     3541 cagaaatgac atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat
     3601 attgtttcgt ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag
     3661 ttgcgactgc tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt
     3721 ctatcccatt taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt
     3781 taaacaaaac gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg
     3841 ctctgtttgg tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga
     3901 agagatggct aagattcctt catctacttc attttcgata atcgtttttt cacttaactg
     3961 ttctgctatt tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa
     4021 aacggcgcct atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg
     4081 tccatctttg acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat
     4141 aatctcttga tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc
     4201 tttttcagag aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa
     4261 aggaagaccg atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac
     4321 aacgataaaa tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg
     4381 tgtcgggtca ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc
     4441 aatgctagag attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat
     4501 caatatggca gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt
     4561 gttctggagc tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat
     4621 ttattgtgaa aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa
     4681 ccttcctata aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt
     4741 ggtatgatag acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag
     4801 acgatttatt ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt
     4861 ggagatgtat tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact
     4921 ttcacaagct tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta
     4981 tttattgcta gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg
     5041 atgccgatga aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa
     5101 gtagctgttg tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt
     5161 gttaccggtt ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag
     5221 cggggaacga aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat
     5281 gaaagcatga taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc
     5341 gcgatcgcaa tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac
     5401 cagacagcaa aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat
     5461 gtgcacattg atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc
     5521 ttgccagttt ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc
     5581 acccctgaag tgtataccga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc
     5641 aaaatcggtt catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca
     5701 agagaagaac tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt
     5761 ggttctatga ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa
     5821 gaagtcgctt ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa
     5881 gaggtcagag acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc
     5941 aaaagagatg cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct
     6001 caagcagaaa aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca
     6061 ttaaaagaaa aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca
     6121 aaagcagacc aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag
     6181 gaaatgcaga ttaaaatcgt agaacgccaa aaacaaatcg aactggaaga aaaagaaatc
     6241 acacgtcgtg aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac
     6301 gctaaagaac aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa
     6361 cgattccgtg tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa
     6421 gcagaagccg aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc
     6481 gccaatgcct tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta
     6541 ccgcaattga tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc
     6601 gatacaggaa gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca
     6661 aatttgcttt cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa
     6721 atcgtagaga atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca
     6781 aaagaggcag aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc
     6841 aaaaatggag gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga
     6901 aaattagtcc tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt
     6961 tgttcttagc aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt
     7021 cttttctgtt tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat
     7081 aaaagtatct agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa
     7141 cgcaatcagc aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc
     7201 tgacacgacg atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc
     7261 attgtgaaaa tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt
     7321 gccgatgatc ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa
     7381 aacagcagat aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat
     7441 acataaaaaa acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa
     7501 ttgcatagag attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag
     7561 ctagtttttc attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg
     7621 aatgcgaaca gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt
     7681 atcgctgttg tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa
     7741 gcgctctaca ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac
     7801 agcactgttc agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt
     7861 cctgttcatc ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt
     7921 tttggtcact cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc
     7981 gggattaatg gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg
     8041 tacgaagcag ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag
     8101 acaaatggca gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca
     8161 tttactgaca ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg
     8221 gctcagacag ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt
     8281 gattaaagac atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca
     8341 tatctatggt ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca
     8401 aattttaaca cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag
     8461 tcgccctgaa ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt
     8521 tattgaaatc aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca
     8581 gctaatattc aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt
     8641 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg
     8701 ctccttgtga ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa
     8761 agctttcctc tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt
     8821 ggctgttctt attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt
     8881 ttccatagaa cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag
     8941 cgattgggca agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct
     9001 tcagatgtat tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca
     9061 cgatcagcac gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg
     9121 acttttggca tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta
     9181 cgcaagatag tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca
     9241 actacttcag gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata
     9301 tccaacagag ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct
     9361 ttgtaactgc cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt
     9421 attgagcgta actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc
     9481 aaattctttt cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg
     9541 gctttttcac atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc
     9601 tgtctatttg gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga
     9661 tagcttccgt aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa
     9721 gttctggaag caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca
     9781 tgtgcgatgg taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc
     9841 ttcatagttg gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa
     9901 agaggagagg acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg
     9961 atcaaaactt aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga
    10021 tcttattatg agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata
    10081 cttgctgatc agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc
    10141 tgaagcgatg cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa
    10201 gattttgcgt caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa
    10261 gcaaatcaaa acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct
    10321 gatccctttg aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca
    10381 atatgatgaa tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa
    10441 acagatcgaa ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac
    10501 ttacgttcat gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata
    10561 aatttttagg atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta
    10621 gcgatgatga ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa
    10681 caactgctag taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa
    10741 caaaagatcg tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt
    10801 tggttggtcc agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat
    10861 taagtaacgg atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg
    10921 ctgctgaagc agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc
    10981 aacaacaagt tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag
    11041 aggaggcgac aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca
    11101 caggtctggc agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat
    11161 tggatgcaag ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt
    11221 atggatttat cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac
    11281 cttggcactt acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga
    11341 cgctagaaga atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa
    11401 agtggaaaaa gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag
    11461 tgatactctt agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc
    11521 agcaagaaaa tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc
    11581 aagaactaca agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag
    11641 aatcaaattg gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg
    11701 cttatgggga tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga
    11761 taactatcca aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata
    11821 cacaactgtt cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg
    11881 ccaccaacta tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg
    11941 gttatgcgca aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga
    12001 gatagcaaag gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt
    12061 gagaaaagat atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt
    12121 tggaaaattg atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa
    12181 tgcgaatgca gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat
    12241 tattgaggca gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc
    12301 tcgttcttcc tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa
    12361 cggtcctacg cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat
    12421 cagtacggta acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat
    12481 tatcaatatc agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata
    12541 tcctgaactt cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat
    12601 tcaagcagga gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg
    12661 taaaatggaa aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa
    12721 aagacgagga agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt
    12781 cagaattcaa aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag
    12841 ttaaaacctt ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc
    12901 tttctataga atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc
    12961 taaattgtcg gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg
    13021 cttgtgcatc gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg
    13081 ttttttaggg aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg
    13141 gcagttgtct gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag
    13201 tgtttttagc gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg
    13261 ttcatcctag gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt
    13321 ttaataaata tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg
    13381 tgcagagaat gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct
    13441 tgacggcttg tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt
    13501 cgcaagtccg acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc
    13561 atgtgacgat cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac
    13621 tagcagcatc agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca
    13681 tcaatcagat tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg
    13741 cagatgaatc ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg
    13801 gggtgaattc gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg
    13861 aaccagcttc aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag
    13921 aattaggaat ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa
    13981 taccacaagt tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg
    14041 gatttgaaaa tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca
    14101 aaggggccca tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg
    14161 cggttaggga tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat
    14221 tgtcaggggc tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag
    14281 atggagaatc tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag
    14341 cccataaaga agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg
    14401 aaattttaaa aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc
    14461 gtgcagatga tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg
    14521 acaacggcga agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca
    14581 atcttttagt tgaaaaagtc atttatgaag aagtaacagc agaaggaagg catgaaaaaa
    14641 gaaaaactaa aacggttctt ggatcaagac aatgtggtcg gttacgtatt tgcttctcca
    14701 ttcatcctag gatttctatt gataacagtg ataccgatga tcttgtcact ttattattcg
    14761 ttcaccgatt atcgtttggg aaatccagtc tcatggatcg gcttggataa ttatataaag
    14821 atgctgagtg atgcccgttt tttgaactcc atccgagtca cttttctcta tgtatttgca
    14881 tctgtacctg tgaaactgat ctttggtcta ttgattgctt atcttttaac gaggaatatc
    14941 aaaggaacga ctttctttcg ttctttgtat tatgtgccta gtttgatcgg tggcagtatt
    15001 tctatcgctc tagtttggcg ggagttgttt tctaaaaacg ggttagtgaa tattttcctg
    15061 gaacgattag gcgcacctgc gctttcctgg tttagcgatc aaaaactggt catgatccct
    15121 cttgttttga tgtctgcttg gcaattcggt tcggcaatga tcatctttgc tgcaggtatc
    15181 aaagagatcc caggctcgta ttatgaagcg gcagagatcg atggagcgaa caaatggcag
    15241 acgttttaca agatcacgtt acctcttctt tcgccgatca ttttgtataa cttggttatg
    15301 cagacaatcg cggcgtttat gacttttacg caggcattta tcattaccca aggtggtccg
    15361 aatgatgcca cgaactttta tgctctttat gtgtataacc aagctttttc ttttaattct
    15421 atggggtatg ctagtgctat ttcgtggatc atgctgttga ttatgggagc tgtcaccttt
    15481 ctgattttca aatcatcaaa aaaatgggtc gttgtgtgaa tgagaggagg aagaaatgaa
    15541 taatacagta acgaaatggt ttcaatacgg ttttattttg ctattaggtc tagtcatgat
    15601 ttatccggtc ttatggatga tcgcaggttc ttttaaaacg gatcaagaga ttatgagtgg
    15661 ttcgctgaat ttgattccag agacttttca atgggaaaat tacgcaaaag gatgggcggg
    15721 gtttgctgga atttcatttt ttactttctt taggaattcg tttgtcatct ctgcagtttc
    15781 gacgataggg acagttctga gctcgacttg tattgcttac gcattatcga gaatcgattt
    15841 caaaggaaaa agattctggt tcgtcgtcat gttagtaacg atgatgattc ctgcacaagt
    15901 gattttgatt ccgcagttta ttatttacaa tcggctgaat ttggttggga cgtatgtgcc
    15961 gctgatttta ccacattttt ttggacaagc atttttcatt taccagatca tgcaattcat
    16021 ggtcagcatc cccaaagagt tagatgaatc agcgattatt gacggatgca gcaagtatag
    16081 tgtctttacc aaaattattt ttcctttgct gaaaccttcg atcatcacga cgatcatcat
    16141 ccagttttat tggaaatggg acgacttcat gggaccattg atttatttaa acaagccgag
    16201 atcttacacg gtatcgattg ccatcaaatt atttgcagac gctggatcta gtacgactta
    16261 cgcgagtatg tttgcgatgt ctacgttgtc gctgctaccg gtttttctga ttttcttatt
    16321 cttcaataaa tatcttgtcc aggggatcag tacaagcggt ctgaagggct gaatatgaaa
    16381 aagccaatag aagagatgtt tctttacaag ctcaactact atgtctgtcg gattttttta
    16441 accagtctta tcagtaatgc tcttttcgct ttagttctag gatgtttgtt cactttagga
    16501 ttagtgctgt ctgctcgtgt tgtctatctg atttattttt tgatgatgta tccttcttat
    16561 tatctatctg tccattactt ggcagaaaaa aaggtatgtt ggaggaaagg gaaaatcaaa
    16621 cggatgtgtt tcatcattac gttgtttcag ctgtttgtca ttatttttgc agttgattgg
    16681 tatttattag tgggaaagat tggagcttgg tatctgattc cttttctatt gtgtctgttg
    16741 ctttttgtat cccatagttt ttttagtacg ctaattgata tctgcaaaga agataaagca
    16801 gatagtcttg tttatttgat caaaaggaat atctggctgt tgtcaaaaaa cgtcaagggg
    16861 tttctgtggt atttatgcct tttgtatgga ctgttctggt gtatacagcc tgatttttta
    16921 ctcactgcat tactgtatcc agggctgcca tttgtcacag tattttgctt aaaagaaaag
    16981 gagaatgact ttaaatgaag aaaaaatatg tacaagtcgg acttggtggg agagcgcgtt
    17041 ttttttatga ggcaattgcc tctacgtatc atcaaacttg tgaactagtc ggtttttgtg
    17101 atgtatctca aacaaggatg gattatgcaa atcggatact tgtgaacaag tatggacaga
    17161 aaaaagtaaa gacatgtatg ccatcacaat ttgagcaact actggatgac gtacggccgg
    17221 ctgaagtcat tattacgtca gtagatcgaa cgcatcatga ttatatcatt cgtgcaatga
    17281 aaaaaggata tgatgttatt tgcgaaaaac ctctgactat cgatgaggaa aaagctcaag
    17341 aaataataac ggtccaaaaa cagacgggaa gaagcctgcg agtcactttt aattatcgtt
    17401 atgcaccgta tcacacgaag ataaaggaac tgatagaaag tgatgtgatt ggtgatgtgt
    17461 tttctatcca cttcgaatgg ctgttaaata cgcaacacgg ggcagattat ttcagaagat
    17521 ggcatcgaaa taagcacaat agcggcgggc ttcttgttca taaatctacc catcattttg
    17581 atctagtgaa tttttggttg aacagccgtc cccaaaaggt atttgcattc ggtggattaa
    17641 acttttatgg gatgcataat gcggagaaac gcggagtaga aatattttac agtcgatgct
    17701 atggaagcga actagctaaa gctgatccat ttgctataga cctgtcagcc aatgaggaat
    17761 tgcgagaaat gtatttagaa gcagaaaaag acagtggata tattcgtgac cgcagtgtat
    17821 tttccgatga tatctctatt gaagatacac taggcgtaac ggtcaaatat gaaactggtg
    17881 cgattttgac gtattcgcta aacgcgtata tgccgtggga aggattcaat gtttctttca
    17941 atggcagcaa aggacggatc gaaatgaaag tgatcgaaaa gtcttacatc aatgcaggtg
    18001 gagataaaaa ggatgaagga gcagccgttc ttcagtccat ccatgtctat ccgatgttcg
    18061 gtgagccata tgaggtaatc gtcgaacaag aagcgggcgg acatggcgga ggagatccgc
    18121 gtttattaga tgatctattt ggtgaagaaa aagaagaaga tccgtggaat cgggcagcaa
    18181 ctcacgtaga tggtattttg tcgattttga caggaatcgc agcaaatcat tctattgctt
    18241 caggaaaagc agtagtgatt gacgagcttg tcagcttaaa agaataagaa tagatttacg
    18301 gaaattactt cccgtagttt ataaaatctt gccttattgc cgataggctg tcttttgaac
    18361 actatttatt cggaaaaagg ggctatgaca tgctttttgt catgccttct ttttatgtgg
    18421 gctgcttttt tagataggag aaaataaaaa taactattta aacccgaaca aaaaggaata
    18481 atgcattaaa aatcattaga ttttacaaaa aaataagtaa accacgttgt cagtttcaga
    18541 aagtagtgtt agactgtttt caatatttga aacaacattt tttattcaaa tatggcaaac
    18601 tgccttcacg tgcaaaacag ttgcaagaaa atgaatttac actattatta gttttacata
    18661 cctacttata tatcgtcata atatggatga cagtttctag ccagtaccgt aaatgctgga
    18721 ctataagtaa aagattggct atttcaccct tcttttctta tgataaagtg gagggtgttt
    18781 ttctttaaca gaaacatagt aggtttttgc acaaaagcgg ttatttcggt attaataaat
    18841 gataaaggag caaataaagt gaaagaatta gttgaacgta ttcaaaaaga cggacgtata
    18901 ttaggcgagg gcgtattaaa agtcgacagc ttcgtcactc accaagtaga ccctgtattg
    18961 atggaacaaa tgggaaaacg gtttgcagaa gtatttaaag aacaaggaat cacgaaagtt
    19021 gtgaccattg aagcttctgg gatcgcacca gctttatttg ctgcaagaga attagatgtt
    19081 ccaatgatct tcgctagaaa agcaaaaagt ttgacgatgg atgaagaatt attgacagct
    19141 tctgtttatt cttttacgaa acaagtgaca agtacgattt ctatttctcg taaattttta
    19201 tcttcagaag acaaagtatt gattatcgac gatttcttgg caaacggaca agcggcaaaa
    19261 ggattgatcg aattatgcca gcaagctgga gcgcaagtag aaggaatcgg gatcgtcatc
    19321 gaaaagtctt tccaagatgg acgtcagtta cttgaagaaa tgggattgag agtcgtttct
    19381 cttgcgcgta ttgcttcctt gacgaatgga caagttgagt ttcttgagga ggacgcataa
    19441 tcgtggaaaa agaaacacaa aatggaaaag ctgcggtttt agggctgcag catctgctag
    19501 cgatgtatgc aggagcagta gcggtgcctc ttttgatcgg tacaggacta ggctttaatc
    19561 aagaacagat gacttatttg atttcaattg atatctttat gtgtggatta gcgacattat
    19621 tgcaattgac agttaaccga tttttcggaa ttggcttgcc tgtcgtttta ggatgtgcga
    19681 tccaagcagt agctccactg atcttgatcg gaaccaatga aggagtagga gctatctacg
    19741 gatctattat cgcttctggg atctttgttg tgttagtttc tggtgtcttt tctaaaatta
    19801 aaaaactatt ccctcctata gtaacaggaa cggttatcac cgttattggt ttgactttga
    19861 ttcctgtagc aatcgagaaa atgggtggag gtaatgcaac agcagctgat tttggtgaca
    19921 aaacaaatct gctacttgca tttgtgacga ttctcctaat tgtaggcgtg caaatgctgg
    19981 caaaagggtt cattcgttca atcgcggtat tgattggttt agtcggagga agcatcttag
    20041 ctgctttcat gggaatgatt gatctgagcg cgattggaga tgcgccgttg ttccatgttc
    20101 cacaaccctt ttattttggt aaaccaactt tcgacgtatg gtcgatttta ctaatgatca
    20161 tcatttccat ggtcagcatg gtagaatcaa caggtgtata ttttgcactt ggtgacatta
    20221 ctgggaaaaa tgttggagaa gaagaattga aaaagggata tcgtgcagaa ggacttgccg
    20281 ttatcttagg cggtattttc aatacattcc cttatactgg cttttctcaa aatgtcggat
    20341 tggttcaact ttcgggaatc aaaacaagaa aacccatcta tttctctgca ttcttcttga
    20401 ttgttttagg attattccct aaaatcggtg cgttagcaca aatcatccct gagccggtac
    20461 ttggtggagg aatgttagtc atgttcggga tggtggcagt ccaaggaatc cgtatgctgt
    20521 tggaagttga tttcagcaat gataaaaatc tattgattgt tgctgtttct gttggatttg
    20581 ggctgggttt caatgttacg ccgacattat tcagtcaaat gccgcaaaca ttgcaaatgt
    20641 tcactggaaa tggcatcgta atgagtagca ttacggctat catattgaat ttgatcttta
    20701 atggattgaa aaaagatgaa gaaatattgg aagaagcctt caagaagtaa tagagcaaaa
    20761 gaaaggttgt gaaatcagcg ttttgacctg attgttagag aaacaaacgg aaaaatagtt
    20821 cctcgtattt tgccgtttgt ttgagctaat accgagggtc agctgattga acaccgttta
    20881 tccataaaaa ggaatgtgac ctaacttttg tcacattcct ttttttcatc tttaataaac
    20941 acgaacaata aaatgttttt ctttgttaac attcgaaatt tctttgacat caaaaaaagt
    21001 gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa caatgacagc aaaaatctct
    21061 gtgattatgg ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat
    21121 gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg
    21181 tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg
    21241 ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta
    21301 ccggtccaat cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct
    21361 ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta
    21421 cttgcgatac agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga
    21481 cgttcaatgt tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt
    21541 gcctggttca acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag
    21601 tgcaaaagaa atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc
    21661 acaagttgca gattggcaca ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct
    21721 tgccagaaga acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa
    21781 tgcgatttta cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga
    21841 ccgcctgtta gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac
    21901 gatcgtcagt ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt
    21961 gaaaaccact cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga
    22021 tctagctcct gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc
    22081 gtttgaaaaa gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt
    22141 tccggtcgtg gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc
    22201 gattgatcag gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt
    22261 gagcctttca ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt
    22321 gaatgaatta gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat
    22381 gagtcagttc gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg
    22441 tttgataagc aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg
    22501 cttgattgag aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa
    22561 aggtcgcaag atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga
    22621 aatcaaacaa acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg
    22681 ccctgaaatg gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga
    22741 gattttagca gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa
    22801 aattcgtgag catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg
    22861 ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg
    22921 ggtccattat ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa
    22981 gcaagcaaat gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa
    23041 agcaaaagaa cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc
    23101 aactactttt ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg
    23161 atttgaacat gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt
    23221 cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca
    23281 agaaatctct actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc
    23341 gcttattgct acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga
    23401 aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca
    23461 caaaaggaat ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca
    23521 catggtcact gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc
    23581 agcagaacga atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt
    23641 tggcaatatc gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc
    23701 aacatttggt ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac
    23761 aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt
    23821 gcagttccgc ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga
    23881 aatagacgat gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg
    23941 agtaggtttg gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa
    24001 taaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct
    24061 tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata
    24121 caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg
    24181 gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat
    24241 ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc
    24301 gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt
    24361 tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc
    24421 tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa
    24481 aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat
    24541 ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat
    24601 ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg
    24661 tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac
    24721 aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa
    24781 gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt
    24841 tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt
    24901 ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga
    24961 ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta
    25021 caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt
    25081 ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat
    25141 gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaaa
    25201 ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa
    25261 ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat
    25321 ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt
    25381 acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc
    25441 tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt
    25501 atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag
    25561 taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat
    25621 actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt
    25681 gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg
    25741 aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat
    25801 atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag
    25861 tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat
    25921 ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct
    25981 gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga
    26041 cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca
    26101 ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga aataatctat
    26161 cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta
    26221 aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac
    26281 attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt
    26341 gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa
    26401 aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc
    26461 aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa
    26521 gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg
    26581 agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga
    26641 cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta
    26701 gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga
    26761 gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt
    26821 gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta
    26881 gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc
    26941 ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta
    27001 ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc
    27061 atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa
    27121 gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg
    27181 gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata
    27241 ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa
    27301 gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca
    27361 ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga
    27421 gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt
    27481 aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg
    27541 ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct
    27601 cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa
    27661 gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact
    27721 tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg
    27781 agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg
    27841 tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt
    27901 gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa
    27961 ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg
    28021 ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca
    28081 atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact
    28141 actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat
    28201 tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac
    28261 tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga
    28321 ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca
    28381 gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt
    28441 gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa
    28501 gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa
    28561 atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag
    28621 gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg
    28681 tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc
    28741 gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag
    28801 atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag
    28861 aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc
    28921 agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga
    28981 tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact
    29041 ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata
    29101 aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa
    29161 cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga
    29221 aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt
    29281 ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg
    29341 attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca
    29401 gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga
    29461 atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt
    29521 tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa
    29581 aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg
    29641 ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg
    29701 atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag
    29761 gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg
    29821 ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca
    29881 aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga
    29941 tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt
    30001 atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat
    30061 cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca
    30121 aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc
    30181 aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat
    30241 ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg
    30301 aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg
    30361 atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta
    30421 gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga
    30481 tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac
    30541 tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta
    30601 ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt
    30661 cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta
    30721 tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa
    30781 atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat
    30841 atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca
    30901 caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt
    30961 gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca
    31021 gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt
    31081 tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa
    31141 cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt
    31201 atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag
    31261 catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag
    31321 aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga
    31381 tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt
    31441 atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg
    31501 gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg
    31561 accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg
    31621 acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag
    31681 tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt
    31741 atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat
    31801 catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat
    31861 cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa
    31921 aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac
    31981 ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat
    32041 tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag
    32101 tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga
    32161 ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca
    32221 aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata
    32281 cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa
    32341 acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct
    32401 tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa
    32461 taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt
    32521 agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc
    32581 gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa
    32641 tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat
    32701 tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt
    32761 actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt
    32821 aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt
    32881 cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc
    32941 tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat
    33001 cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga
    33061 gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga
    33121 atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca
    33181 agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca
    33241 ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg
    33301 tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc
    33361 gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga
    33421 tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc
    33481 tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt
    33541 cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat
    33601 ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc
    33661 agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg
    33721 gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt
    33781 gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg
    33841 agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc
    33901 gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc
    33961 ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt
    34021 aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc
    34081 gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga
    34141 tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga
    34201 tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt
    34261 tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt
    34321 tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt
    34381 tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca
    34441 gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga
    34501 tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt
    34561 ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga
    34621 gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat
    34681 taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg
    34741 cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc
    34801 atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa
    34861 catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga
    34921 tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga
    34981 aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg
    35041 aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca
    35101 gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag
    35161 ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca
    35221 atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc
    35281 atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta
    35341 tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc
    35401 aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag
    35461 tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta
    35521 atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa
    35581 gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg
    35641 ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg
    35701 gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga
    35761 gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt
    35821 tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg
    35881 attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta
    35941 ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga
    36001 tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc
    36061 cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg
    36121 taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt
    36181 tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat
    36241 aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac
    36301 ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca
    36361 gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta
    36421 agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg
    36481 attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat
    36541 tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa
    36601 tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc
    36661 tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg
    36721 acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat
    36781 tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa
    36841 tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa
    36901 tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta
    36961 agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat
    37021 ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc
    37081 aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata
    37141 attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa
    37201 gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg
    37261 ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag
    37321 tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc
    37381 gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga
    37441 atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat
    37501 cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgcgg taatacgaat
    37561 aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg
    37621 caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc
    37681 aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc
    37741 tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag
    37801 tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc
    37861 atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact
    37921 ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc
    37981 cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg
    38041 aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc
    38101 ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag
    38161 aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt
    38221 gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt
    38281 gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat
    38341 attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc
    38401 taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct
    38461 aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa
    38521 gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg
    38581 gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag
    38641 tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt
    38701 tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag
    38761 ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc
    38821 ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag
    38881 gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt
    38941 gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg
    39001 cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata
    39061 aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac
    39121 cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta
    39181 gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg
    39241 agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt
    39301 gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg
    39361 tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc
    39421 ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa
    39481 tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat
    39541 tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg
    39601 attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta
    39661 gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa
    39721 caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt
    39781 actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca
    39841 agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca
    39901 taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg
    39961 gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc
    40021 aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt
    40081 aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt
    40141 ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga
    40201 aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc
    40261 ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa
    40321 ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt
    40381 tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa
    40441 agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc
    40501 actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt
    40561 ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc
    40621 tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga
    40681 tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt
    40741 ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc
    40801 tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat
    40861 cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt
    40921 caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac
    40981 agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt attataaaga
    41041 atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag
    41101 aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct
    41161 ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg
    41221 tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca
    41281 tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg
    41341 aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat
    41401 ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat
    41461 tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac
    41521 ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa
    41581 gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa
    41641 gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc
    41701 atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta
    41761 cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt
    41821 cgcgggatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga
    41881 gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc
    41941 ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg
    42001 atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag
    42061 atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc
    42121 attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg
    42181 atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta
    42241 ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa
    42301 acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt
    42361 ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg
    42421 atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc
    42481 aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc
    42541 tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa
    42601 gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct
    42661 tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg
    42721 ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg
    42781 tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt
    42841 actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc
    42901 agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc
    42961 gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt
    43021 ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt
    43081 tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt
    43141 ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt
    43201 gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag
    43261 aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc
    43321 ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta
    43381 tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa
    43441 aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc
    43501 agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc
    43561 tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc
    43621 gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat
    43681 tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat
    43741 gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt
    43801 agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta
    43861 ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca
    43921 ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat
    43981 tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa
    44041 agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg
    44101 taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata
    44161 gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag
    44221 tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg
    44281 gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa
    44341 tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct
    44401 ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc
    44461 atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa
    44521 cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg
    44581 gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga
    44641 caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc
    44701 gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc
    44761 gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg
    44821 gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat
    44881 tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat
    44941 ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg
    45001 gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt
    45061 ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc
    45121 tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg
    45181 aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa
    45241 gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc
    45301 ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga
    45361 gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa
    45421 cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg
    45481 tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac
    45541 gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc
    45601 aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca
    45661 aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga
    45721 gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt
    45781 agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg
    45841 gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg
    45901 cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc
    45961 tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt
    46021 atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca
    46081 taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt
    46141 gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat
    46201 tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat
    46261 acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa
    46321 aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc
    46381 gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt
    46441 taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg
    46501 actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg
    46561 ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt
    46621 agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat
    46681 cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa
    46741 aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac
    46801 agcttggatt taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga
    46861 aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt
    46921 gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt
    46981 agaaaataga aaaaatgcag atgaaaaata aaacgctttc cttattgtga ttttattcac
    47041 taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta
    47101 gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca
    47161 gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg
    47221 tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca
    47281 atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt
    47341 tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc
    47401 gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac
    47461 caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg
    47521 ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc
    47581 aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag
    47641 ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag
    47701 gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat
    47761 cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat
    47821 tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa
    47881 aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg
    47941 atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg
    48001 gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg
    48061 atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga
    48121 tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag
    48181 tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa
    48241 aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag
    48301 aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat
    48361 ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt
    48421 tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa
    48481 gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag
    48541 tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat
    48601 tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg
    48661 cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga
    48721 attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat
    48781 tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc
    48841 ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga
    48901 agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat
    48961 tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga
    49021 aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat
    49081 cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc
    49141 gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc
    49201 cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt
    49261 ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga
    49321 aaagaaggga cgaaacaaag tcatggctta tcaatttaaa tt
//
LOCUS       DARBEG010000013        45475 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000013 DARBEG010000000
VERSION     DARBEG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.013"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCK20_000726"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCK20_000726"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG1144813.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCK20_000727"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCK20_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG1144814.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCK20_000728"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCK20_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG1144815.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCK20_000729"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCK20_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG1144816.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCK20_000730"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCK20_000730"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG1144817.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCK20_000731"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCK20_000731"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG1144818.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SCK20_000732"
     CDS             complement(8053..8523)
                     /locus_tag="SCK20_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG1144819.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SCK20_000733"
     CDS             complement(8569..8994)
                     /locus_tag="SCK20_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG1144820.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SCK20_000734"
     CDS             complement(9061..9330)
                     /locus_tag="SCK20_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG1144821.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="SCK20_000735"
     CDS             complement(9341..10936)
                     /locus_tag="SCK20_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1144822.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SCK20_000736"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SCK20_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG1144823.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="SCK20_000737"
     CDS             complement(14489..15049)
                     /locus_tag="SCK20_000737"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG1144824.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="SCK20_000738"
     CDS             15402..16376
                     /locus_tag="SCK20_000738"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1144825.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="SCK20_000739"
     CDS             16546..17946
                     /locus_tag="SCK20_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG1144826.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="SCK20_000740"
     CDS             complement(18024..18746)
                     /locus_tag="SCK20_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1144827.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="SCK20_000741"
     CDS             complement(18825..19331)
                     /locus_tag="SCK20_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1144828.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="SCK20_000742"
     CDS             19454..21253
                     /locus_tag="SCK20_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG1144829.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="SCK20_000743"
     CDS             complement(21321..22895)
                     /locus_tag="SCK20_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1144830.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SCK20_000744"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SCK20_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG1144831.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SCK20_000745"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SCK20_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG1144832.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="SCK20_000746"
     CDS             complement(24672..25310)
                     /locus_tag="SCK20_000746"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG1144833.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="SCK20_000747"
     CDS             complement(25311..25961)
                     /locus_tag="SCK20_000747"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG1144834.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SCK20_000748"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SCK20_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG1144835.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SCK20_000749"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SCK20_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG1144836.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="SCK20_000750"
     CDS             complement(29104..29844)
                     /locus_tag="SCK20_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG1144837.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SCK20_000751"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SCK20_000751"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG1144838.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="SCK20_000752"
     CDS             complement(31215..32153)
                     /locus_tag="SCK20_000752"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG1144839.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="SCK20_000753"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="SCK20_000753"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1144840.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SCK20_000754"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SCK20_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG1144841.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="SCK20_000755"
     CDS             complement(35313..35618)
                     /locus_tag="SCK20_000755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1144842.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SCK20_000756"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SCK20_000756"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG1144843.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="SCK20_000757"
     CDS             complement(36724..37008)
                     /locus_tag="SCK20_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144844.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="SCK20_000758"
     CDS             complement(37026..37916)
                     /locus_tag="SCK20_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG1144845.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="SCK20_000759"
     CDS             complement(37936..38154)
                     /locus_tag="SCK20_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG1144846.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="SCK20_000760"
     CDS             complement(38124..39824)
                     /locus_tag="SCK20_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG1144847.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="SCK20_000761"
     CDS             40091..40864
                     /locus_tag="SCK20_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144848.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SCK20_000762"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SCK20_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG1144849.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="SCK20_000763"
     CDS             complement(41688..42659)
                     /locus_tag="SCK20_000763"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1144850.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SCK20_000764"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SCK20_000764"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG1144851.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SCK20_000765"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SCK20_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG1144852.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT        12385 a         9870 c         8232 g        14988 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa tttta
//
LOCUS       DARBEG010000014        43212 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000014 DARBEG010000000
VERSION     DARBEG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.014"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCK20_000766"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCK20_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG1144853.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCK20_000767"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCK20_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG1144854.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCK20_000768"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCK20_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG1144855.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="SCK20_000769"
     CDS             1642..2274
                     /locus_tag="SCK20_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1144856.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="SCK20_000770"
     CDS             complement(2276..3181)
                     /locus_tag="SCK20_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1144857.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="SCK20_000771"
     CDS             complement(3266..3925)
                     /locus_tag="SCK20_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1144858.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="SCK20_000772"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="SCK20_000772"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG1144859.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="SCK20_000773"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="SCK20_000773"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG1144860.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="SCK20_000774"
     CDS             6168..7253
                     /locus_tag="SCK20_000774"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG1144861.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="SCK20_000775"
     CDS             7250..8314
                     /locus_tag="SCK20_000775"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG1144862.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="SCK20_000776"
     CDS             complement(8563..9366)
                     /locus_tag="SCK20_000776"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG1144863.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCK20_000777"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCK20_000777"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG1144864.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="SCK20_000778"
     CDS             complement(10244..11251)
                     /locus_tag="SCK20_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1144865.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="SCK20_000779"
     CDS             complement(11258..11902)
                     /locus_tag="SCK20_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1144866.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="SCK20_000780"
     CDS             complement(12002..12760)
                     /locus_tag="SCK20_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG1144867.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="SCK20_000781"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="SCK20_000781"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG1144868.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="SCK20_000782"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="SCK20_000782"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG1144869.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="SCK20_000783"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="SCK20_000783"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG1144870.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="SCK20_000784"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="SCK20_000784"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG1144871.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="SCK20_000785"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="SCK20_000785"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG1144872.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="SCK20_000786"
     CDS             18158..19027
                     /locus_tag="SCK20_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG1144873.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="SCK20_000787"
     CDS             complement(19153..20052)
                     /locus_tag="SCK20_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144874.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="SCK20_000788"
     CDS             complement(20217..21041)
                     /locus_tag="SCK20_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1144875.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="SCK20_000789"
     CDS             complement(21052..22347)
                     /locus_tag="SCK20_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG1144876.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="SCK20_000790"
     CDS             complement(22363..22842)
                     /locus_tag="SCK20_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG1144877.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="SCK20_000791"
     CDS             complement(22848..23876)
                     /locus_tag="SCK20_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG1144878.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="SCK20_000792"
     CDS             complement(23893..24999)
                     /locus_tag="SCK20_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1144879.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="SCK20_000793"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="SCK20_000793"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG1144880.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="SCK20_000794"
     CDS             complement(28130..28510)
                     /gene="yajC"
                     /locus_tag="SCK20_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG1144881.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="SCK20_000795"
     CDS             complement(28607..29752)
                     /gene="tgt"
                     /locus_tag="SCK20_000795"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG1144882.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29970..30668
                     /locus_tag="SCK20_000796"
     CDS             29970..30668
                     /locus_tag="SCK20_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1144883.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30836..33475)
                     /locus_tag="SCK20_000797"
     CDS             complement(30836..33475)
                     /locus_tag="SCK20_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1144884.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(34094..35125)
                     /gene="queA"
                     /locus_tag="SCK20_000798"
     CDS             complement(34094..35125)
                     /gene="queA"
                     /locus_tag="SCK20_000798"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG1144885.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35318..36493
                     /locus_tag="SCK20_000799"
     CDS             35318..36493
                     /locus_tag="SCK20_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1144886.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36497..37138
                     /locus_tag="SCK20_000800"
     CDS             36497..37138
                     /locus_tag="SCK20_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144887.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37135..38052
                     /locus_tag="SCK20_000801"
     CDS             37135..38052
                     /locus_tag="SCK20_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1144888.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            38049..38720
                     /locus_tag="SCK20_000802"
     CDS             38049..38720
                     /locus_tag="SCK20_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144889.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39261..39749)
                     /locus_tag="SCK20_000803"
     CDS             complement(39261..39749)
                     /locus_tag="SCK20_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG1144890.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39950..40216)
                     /locus_tag="SCK20_000804"
     CDS             complement(39950..40216)
                     /locus_tag="SCK20_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1144891.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40216..40479)
                     /locus_tag="SCK20_000805"
     CDS             complement(40216..40479)
                     /locus_tag="SCK20_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1144892.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            40955..41140
                     /locus_tag="SCK20_000806"
     CDS             40955..41140
                     /locus_tag="SCK20_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1144893.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41282..43105)
                     /locus_tag="SCK20_000807"
     CDS             complement(41282..43105)
                     /locus_tag="SCK20_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG1144894.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
BASE COUNT        12316 a         9391 c         7909 g        13596 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt atagaaaaat atataaagtt atttattatt atttatatga cacacccgat
    28021 tattcaatat tgagcaatat atcccatgct cttatctctt tcttttttga ataaaaaatg
    28081 aggtaggaca tctttctgcc ctacctcgtc tttaaataag ctttgatact tatttttctt
    28141 ccttctcttc tgtctcttcc agcgtttctg ttgtttcagt ttttacttca actgtctcag
    28201 ctggttcttc tgttgtttca acagtagtcg ttgcatcatt tgttacgaca gtccctggtt
    28261 tgaccgtttt gattgctgca cgatcgtact caaggaagat cccttcgcag tctaaaacaa
    28321 ctgtccgttt gtcattgtcg atttctgaaa caactccatg taatccgccg atcgttacga
    28381 ctgaatcacc tggtttcatg ctatctaaca agttttgacg ttcttgctgt tgtttctttt
    28441 gcgaacgtga cataaaaaac cacattgcta atagcgcgat aaacatcaca agcattggta
    28501 atgttcccat ttttttcacc tcactaattc tgtatctaca aaaaacactt tagcagaaaa
    28561 atgctgataa cactagtttt ttacttaatt tttaaaagat tttcctttag aagctttttg
    28621 cgttttcttt attgaacccg tattcttcaa aaaaggcttc tctaaattca agcaaattat
    28681 catccatgat tgcttgacgt acttgtttca ttagattcaa caagaagtat aaattgtggt
    28741 aagaagtcaa acgaatccca aatgtttcat cacatttgat caaatggcgg atataggcac
    28801 gtgtatagtt tttacatgta taacaatcac atttctcatc aattggacgg aaatcatggg
    28861 catatttcgc atttttcact actagacggc cttgagaagt catacatgtg ccatttcgag
    28921 cgattcgagt aggcaagaca cagtcaaaca tgtctactcc acggataaca ccatcgatca
    28981 atgaatcagc agcaccgaca cccatcaagt aacgtggttt gttttcgggg atcaacggtg
    29041 tagtgaattc taatacacga ttcatttctg atttaggttc cccgacagat aacccgccga
    29101 ttgagtaacc agggaaatcc atcccgacta gatctttcgc gctttgacga cgaagatctt
    29161 caaaccctgc tccttggatg attccaaata atccttgacg gtctggatta gcatgagctt
    29221 tcaatcctcg ttctgcccat cgagacgtac gttctactga ttttttcacg tagtcgtaac
    29281 tttcatcgaa tggtggacat tcgtcaaaac tcatcatgat atcagagccc aatttatttt
    29341 ggatgttgat tgctttttct ggtgataaaa acatacgcga accgttcaag tgatttttga
    29401 aatgcacgcc ttcttcttcg atattccgca tatcacttaa tgaaaaaact tgaaagccgc
    29461 ctgaatctgt taaaatcggt tgatcccagt tcataaattt atgcagtccg ccagcttctt
    29521 ctactaagtc ttcaccaggg cgtagccata aatgatatgt attacttaaa ataacgccag
    29581 cacccatttc tttcaactct tctggggaca ttgtttttac tgtagctaat gttcctactg
    29641 gcatgaacat tggtgtgggg aaagttccgt gtggcgtgat caattcccct aaacgcgcgc
    29701 cagtatgttt ttctttcttg attaaacgat agcgaatggc aggttgcgtc attcatttcc
    29761 ctactttcta tataaaatat cttgacaact cccaccatca taatcgtttc aagtgctttt
    29821 tgcaagttcc ctcggcttcc ttctacttta tttaatattc ttttttttat ccttttttta
    29881 tgaataacaa aaaaaagaaa aacattatga cagaaatatt ttttgataca tggtatattg
    29941 gtgttattaa agaaaaggag ggaaaaatca tgcaatcttc tgcagaatta aaacgccaag
    30001 caaaagagag tctaaaagga cgttggggac aagctgtttt actaaatcta atccctacat
    30061 tgattggtat tgctactatt cttcttatta tgattcctat tatcttgttt gccaccacca
    30121 tatatgatgg atcagcgagc gtctcagaag cgacaacttc aggaggcggt agttcaggag
    30181 gcggtggcgg aattatttcc agcatcatta gcgctttgtt catgagcggt atttcttgga
    30241 cttatcttga tattttacgt ggacaaaaag atcagatcga accattcaaa gatgcatttc
    30301 gtggattcaa aggtttgttt atcggcggag tgatcagctt agctgtgctg atctcgatct
    30361 ttacaacatt atggggtttg ctattagtta ttcctggaat cattaaaagc tattcttatt
    30421 ctcaaagcta ttttatttac tacgatatcg tccaagaaac aggacaaaca cctaaagtac
    30481 tggatacgat cactgcaagt cgaaaattga tggatggtta taaaggacaa cttttctggt
    30541 tagatctttc atttatcggc tggcatatct tagcattagc aactgctggt attggttatc
    30601 tatggctcag cccatacatt tctgcaacaa aagcagcatt ttatgaacaa ttgccaaaag
    30661 atatttaaaa gagagtgtga cttgtgaccg agggggtggc aggagaacac cgtttatcga
    30721 gaggcagtaa aagaaacatt cagcccatta gcaaaaagga ggtgtgacaa cattcatgtc
    30781 acacctcctt tttgtatagt tccagtaaaa agaaaatcaa cttagaaaca gttaatcatt
    30841 cctcttcttc tgcttccggt aatcgatgga acttgatcag ttcattcacc gcgattggta
    30901 cgatagataa aatccctacc aagaaccagt gattcaaact gatgtgtact aggccgaata
    30961 gttcttgcgt aaatggcagc aaagcaacag ccgttgtaat agctaatgcc agtaacgcca
    31021 tttctaccaa agctttattg gtcgctaaat tgactttgaa gatcgattgg ctgctgcgta
    31081 cattgaacac atgcagaatc gatgaataag ctaagatcaa gaatgcaaca gtttgaccga
    31141 catgctgact tgcgtctacc catgggctga tagtatcgac ataactacct aaataataac
    31201 caaacaaggt tacgatggtg aagacccctg catttatggc aattttttgc aataaacctc
    31261 gagcaaagat cccttcattt ttcggaatgg gtggctgatc catgatgccg tgttccgctg
    31321 gctctcttcc taaagcaaat cctggaagtc catcagcaac tacattgaca aacagtagtt
    31381 gcacagctgt aaatggtgct ccccatccta acagcatcgc aatcaaaaca ataaagatct
    31441 ccgaaatatt acagcttagc aaaaagttga tagctttacg aatattctga taaaccgaac
    31501 gtccttgact gacagcgtct acgatcgtcg caaagttgtc atccgtcaaa atcatatctg
    31561 cggcactttg cgccacatct gttcctgtga tccccatggc acacccaacg ttacttgctt
    31621 tcaacgctgg tgcatcattc acaccatccc cagtcatcgc tacgactgca cccgtacgtt
    31681 gccaagattt gacgatccgg atcttgtctt ctggcgttac ccgagcatag acagagagag
    31741 ctttgacacg cgcatctaat tcttcgtctg ataattgatg cagttctgat cctgaaagtg
    31801 cttctgacgg atcattcaag atacctaatt ctttcgctat ggcactggca gtcaccacat
    31861 gatctccagt gatcatgact gttttgatgc cagccttttt cgctcgcttg attgctcctt
    31921 tactttctgg acgtggtgga tcgatcattc cgatcagacc aatcaaacgt aagtcttttt
    31981 ccagtgcttc agaagaaata tcttgcggtt cttcgtcata agtagcataa ccaacagcaa
    32041 tcactctcag tgcccgttta ccaaagcgat cattaacgat cgccgcttgt tctacatcgc
    32101 ctgaactaaa gcgcggagca agaacatcga atgctccttt ggtgattgag atgtattttt
    32161 cgcccatttg gtgaactgtt gtcatcattt ttcgttcaga atcaaaagga agctcgttta
    32221 ctcgtggata tttttcttct aattctgcct ttgtatgata attctcttca acagccctta
    32281 cgatggctac ttctgttgga ttacctttga taacaaggtc gtcatcttct tgctcgaccg
    32341 tcacatctgt acatagtgca gccattttca atatttccat cgcgctgttc gtcattgaat
    32401 cttcgatatt cgttacttca tcttctttgc tccatacccg gcggacgcgc atttcatttt
    32461 gtgtcagcgt tcctgttttg tctgagcaga taacattagc ggttcctagc gtttcaactg
    32521 ctggcaactg tcggatgatc gcatgtttcc tcgccatctt ctgcacaccg tatgcaagtg
    32581 tcaacgtaac aataacagtc aaggtttccg gtacagctgc aacagccaag gaaatcgaag
    32641 tcatgaacat ctcaaggagt ggttctcctt gcagttcacc aatcacaaat actaaagccg
    32701 cagccgctaa agcgatcata ctgattcgtt tgcctagctg attcaatcgg acctgtaaag
    32761 gtgttttttg catagtattt tcgttcaaaa gatccgcgat cttacccatt tctgtagcca
    32821 tgccaatggc agtgacgatc gctttcccac gacctgcagc aaccgtacat cctttaaata
    32881 ccatattaag ttggtcacct aatgactcat cttcttcagc aataaatgct gcatcttttt
    32941 caactgcttc actttctcca gtcaacgccg attcttctac acgtaattgt gatgcttcga
    33001 tcaaacgggc atcggcagaa atagcatcac cggactctaa aacaagaata tcacccacta
    33061 ccagttgctc tgactcaaca gtggtcactt tgccgtctcg tatgacagtt gccgtttgtt
    33121 tattcatatc ttgcaaggat tccagtgctt tttctgcgtt tccttcttgg acaatcgcca
    33181 ataccgagtt gatcacaacg atagcgatga tcagcagtcc ttcaaataaa tccccatgtt
    33241 ctgtagcaaa tgcggtataa aaggaaatca ccgctgctac aagaaggatg atcgttgtaa
    33301 aatcggacaa gcttcgaaag aattttttga tcatcgattc tttcggcgcc tcatcgaatt
    33361 tattcaatcc ctgctttttt cgccgttcat ccacttcttg tgaagacaac ccagtatctg
    33421 gatctgcttc tgttaccttc tttacttctt ctattgtctg cttgtattct tccatcctat
    33481 tcctccttta aaatcttcgt acaaaaaaag gtacttattt tactataaca tccttttttt
    33541 atttacttca aataaagggc ttatcgatta tttgttaaga aagcgtaaaa tttaaagtgc
    33601 ttatatttgc aaacgctacc aaaggatgct attatataga tgtaaggaag gaggatacca
    33661 tcaaatagac agaagaattt ctcgatagaa cagtgtaagc aaacaaagtt gttagaagta
    33721 caggtttcac ccagacatgc gtgtagagta caaacctata aatagtgaat gcgtatggat
    33781 gtttggatca cgaagtatcc gaaacaagaa gtaatgcgat acgtaacaat tgaataacgt
    33841 aaacgtctga aaattgaata ggaacaagag atgtttcaaa tgttgaagat gtaacgaaaa
    33901 caaaattttt gtttaacatg aagaaattca acaccgtgct ttgcatgtat gatggaaagt
    33961 tacaatcatt tctgtttatt tgaatccttc cgaggtacaa gaaagccgag cttatcgaga
    34021 gctcggcttt ttctttgtca taaaatcttt gtaaagcttt tttgttattc aaaactagcg
    34081 gcttttcata accttatttc acgaacatcg catctccaaa actaaagaaa cgatagcgct
    34141 cttcaatcgc atgctggtaa gcagataaag tcaattctct tcctgcaaag gcactaacta
    34201 acatgactaa agtcgatttt ggcaaatgga aattagttga gaaagcttct accactttga
    34261 attcatagcc aggcgtaatg aaaatatcag tccatccgct gtctgctttg atttctccat
    34321 taaatttcgt tccaatcgtt tctaatgttc gaattgaagt tgttccgaca gccacgattt
    34381 tcccgccatt tttacgtact tggttcaatc gctcagccgc ttcttccgtc aaacggtaga
    34441 attcactatg catatggtgt tcttcaattt tatctacgct gacaggtcgg aatgtaccta
    34501 accctacatg tagtgtcaga tagactaatt caacaccctt tgcttcgatc tcatcaagta
    34561 attctttcgt aaaatgcaat ccagctgttg gcgctgcagc agaaccattt tctttcgcat
    34621 agacagtttg atagcgatct gggtcttcca gacgttcttt gatatatgga ggcaatggca
    34681 tttctcccaa tgattcaaga atctctagaa aaattccttc atactcaaag tcaatgatcc
    34741 gtccgccgtg ttccaattct tctttcacta ctgcttgcaa tctgccatca ccaaaagaga
    34801 ttcttgtccc aactttggca cgtttcgcag gtttgatcaa tgtctcccac gtatctcctt
    34861 gtgtattcgt tagaagaagg acttctaaat gcgcaccagt atctggtttt tctccatata
    34921 aacgagcagg cagaactcgt gtattattca taactagcgc atcaccaggc tgtaactctt
    34981 caatgatttc atggaaatgt ttatcttcca tctctccagt tttccgatcc aaaacaagta
    35041 aacgagaatg atcgcggtct tttaatggtg tttgtgcgat taattcttcc ggcaagtcaa
    35101 aatcaaaatc ttctgttgtt aacatatcgt cactcacttt ctatcatgtg cttcatcatt
    35161 ctatcatgtt tttcgttctg ttggcaaaat cagcactttt tcacttctat tttttgtttt
    35221 cctaaataaa aaaactaccc tatttgtatt ttttcattgt tttaggcata ctagtaatag
    35281 atagttcttc attgaaaata gaaaaagagg tggcaccatg attgaatttc aaaatgtgtc
    35341 aaaaatttat aaaggtggaa aagtcgctgt agaggacgtc aatctttcat ttgaaaaggg
    35401 tgagttcatt tgttttatcg gtacgagcgg aagcggaaaa acgacgacga tgcggatgat
    35461 caaccgaatg actgatccaa cacaagggaa aattttgatt gatggaaaat ccatccaaga
    35521 aattgatcca gtggaattgc gacgtcagat cggctatgtt atccaaagta ttggattgat
    35581 gcctcacatg acgatccgtg aaaatatcac tcttgtccaa aaattgttga aagtcagtca
    35641 agaagaacgg aataaaacag ctgaaaaaat gattgatctt gtggaattgc cccgtgagat
    35701 gttagatcgc tacccacatg aattatctgg tggtcagcag cagcgtattg gagtcgttcg
    35761 tgcccttgca gcgaatcaag atatcatctt gatggacgag ccattcggtg cgcttgatcc
    35821 aatcactcgt gattccttgc aagaccttgt caaagattta caagagcgtt tgggaaaaac
    35881 gatcgtattt gtaacccatg acatggatga agcgattaaa ctagcaagcc gtatcgccat
    35941 catgagtgaa ggccgtgtga tccaatttga tacaccagaa aatattttac gtcatcctgc
    36001 aaacgatttc gtagaagagt tgattggaga agatcgccta ctacaagcca agccaaatgc
    36061 gacaagagtc ggcgaagtta tgctaaatac agcaatcacg attacaccgg aaaaatcatt
    36121 acaagaagca atcaaactga tgagggaaaa acgtgtagat acgttgttag ttaccgataa
    36181 ttcgaatgta ttaaaaggat ttatcgatgt agaaacgttg aataaaaaac gaggaaaagt
    36241 atccagtgtc ggcgacattt tgaataaaca tgttttttac gtaaaagaat ccgcctatct
    36301 tcgtgatacc ttgcaacgca ttttaaaacg cgggctgaaa tacgtgccgg tcgttgatga
    36361 acagaacaaa gtcgtaggta tcctgactcg tgcatcatta gtggatatcg tctacgatgt
    36421 gatttgggga gaagatcccg caactactga tgttgcagag tcagctactc ctgaagaatc
    36481 taaggaggtc tgacctatgc agcagttttt tcatcagcac ggttcagaaa ttctctcaaa
    36541 aggttttgaa catctttata tttcaggact tgctttgatc attggtatca tcattgctgt
    36601 accgcttggt gtcttactga cacgttttcc aaaagctgcc acgattgtga ttggcttaac
    36661 cagtgccttg cagacaatcc cttctcttgc tttattggca ttgatgatcc cgctatttgg
    36721 cgtaggtaaa ttacctgcga ttatcgcact ctttatttat tcattattgc cgattttacg
    36781 taatacgtac attgggatga aaaatgtcga ttggaattat cgggatgtgg caaaaggcat
    36841 ggggatgact gagttccaat ccattttttc cgttgaattg cccattgcta tgccaacgat
    36901 catggctggt attcgtttag ctgccgttta cgtgattgca tgggctacac ttgcctctta
    36961 tatcggcgct ggtggtttag gcgatttgat ttttagtgga ctgaataatt accaaccaga
    37021 tctcattttg gcaggtacga tccctgtaac aattttagca ctattggctg attggcttct
    37081 aggtctcttg gaacaccggc tgacaccgat cgcacttaga gaggagaatg aagaatgaaa
    37141 cgtatactaa aaatgaccat ggttcttttc tctcttctta tacttgcgag ttgttccttc
    37201 cctggtcttg ccagcaatac agatgaagac accatttcga tcacaggtgg aatcacttca
    37261 gaagcacaga tcttagcaag tctagttgcc ggaatggttg aacattatac agataaaaat
    37321 accgcaatca tcaacaatct ggctactaca actatcaacc atcaagcgat gatgaacgga
    37381 gatgcttcaa tttcagcggc tcgctacaca ggaacagatt taacgactac attgaacctt
    37441 cctcctgaaa aagatccgaa aaaagcgttt gctaccgtga aagatgaatt tgagaaaaga
    37501 tacggtcaaa cgtggttccc gtcctatagt tttgaaaata cgtatgtctt tcttgttcga
    37561 aaagacaccg cacaaaaata ccatctatct aaagtcagcg atctgaaaaa tgtagcggat
    37621 gaattagtag ctggtgtaga tacttcttgg atcaatcgta aaggagacgg ttatgacggc
    37681 ttccaagaaa cttatggctt ctccttcaat tcgatattac ctatgcagat tggacttgtt
    37741 tatgacgcag tcgaagcagg aaaaatggat attgttcttg gctactctac ggatggacgg
    37801 atcgccagtt atgatctagt catgttggaa gatgatcgtc aattcttccc accatatgat
    37861 gctgctccag ttgtagacaa caagatatta gctgatacgc ctcatttaaa agaagcctta
    37921 aataagttag gtcataccat cagtacggaa aaaatgcagg aattgaacta cgaagcagat
    37981 aataatctag tggaaccatc agtagtagca caacgtttct tacaagaaaa ccactatttt
    38041 gaagggaagt gaagcaattg gataaaatga atgtctttca acaatttttc tattatttcc
    38101 aggaaaatgg ctcctatatc tttgcacaat ttatccgtca cttcctgatt tctatctatg
    38161 gtgtcctctt tgcagcagtt gtcgggattc cggttgggat catgatttct aggagacgta
    38221 aattagcaaa ctgggtgatt cgtttggcga atatcatcca gacgatccct tctctagcca
    38281 tgatctccat tttaatcatt ggattaggcc tgggagtcaa tgtcgtgatt gttactgtat
    38341 ttttgtattc cctcttaccg atcatcaaga atacgtatac cgggatgatc caagtagaca
    38401 aaaacatcct tgatgtcggc aaaggcatgg ggatgactgc tagacaacga ctattcatgg
    38461 tagagttacc actctctgtt tctgttatca tggcaggcat aagaaacgcg ttagttgttg
    38521 ccatcgggat aaccgcgatc ggtgcattcg tcggtgctgg tggtcttgga gatatcatta
    38581 tccgaggaac caatgcaaca gagggcacat cgattatttt agcaggtgca ttgccaactg
    38641 cattaatggc gatcatcacg gactggcttt taggtatttt ggaaagacgg ctagatccag
    38701 ccagccgtac atcacgctga tgttattgta aaaaacaaaa gcttctgcga ccatttgttg
    38761 gtcgcaaagg cttttttagt tctgtcattt ttaaggcaat aatccactct gattcttccc
    38821 tccttatggg taaagagttg cttttcttag ctatttatcc attccgtata ctccaacttc
    38881 atactgccaa cgggttcata tctgcatcca ccgactaagt ttttttctta tccgctaagg
    38941 cattttgcca atccattttc ccatcactcg tcatggaatt cataccacat gatctctaaa
    39001 tactcgtctg cttgttcttt gaattgggca tcaattctta catcctttgg cgtggctttc
    39061 atttaagcct tttccatcag tcctcgattg ttcgatgcct tcagctgcat catcaaaact
    39121 ggtgactgcc gtatcggtaa cttcgatcat cttttataca atcgggaact tgccatcctc
    39181 atccttggct aaaaacatat ctagccattc cagcttctcg ccatgggagg tttgcggttc
    39241 ctaaaaccgg tacacgattc ttatggactg catccgtcgc aactagactt ggcgatacga
    39301 tgatccttca ccagaagagc acccccgtaa accaattgat cgatatattg ccaataagaa
    39361 aagacattgg cgtaaaagat atcgatccct cgtgatgcat tgccgttgat actgctattc
    39421 atggccgaaa gcgccacgac tttaacttct tccgtttgag tgacattcat gttcggcaat
    39481 gtcgctttgg atacccgctc tgctaaagga acgacactgg tattgtattt cgtatctgta
    39541 tcttttccaa aagaccatgc caacgaatcc tctgggaacc aataagacga ctccggttga
    39601 ttgtccattg cttttttcaa catacttttg gctgtttctc gataaacgac atttccaatt
    39661 gtcttcgcct cactttggcg ataatcgatg gtcgttgcca ctgccaacac taacaacgat
    39721 gctccaattt tactgatttt ccttgtcaca cttcttcctc cttgttttac ataaacccgt
    39781 caacattcca ttcctaaact caaacaatga cccttcaaag gtggagatat aggaacaaat
    39841 taaagaaatc atgcaataaa tccaagaagg ttggttttag aacaaaaaaa gagtctcctt
    39901 tttggggaga ctctttgtta actatctaaa aaggttggct cttctcactt cactcataat
    39961 gtgtccaggc agaataaatt tctacaactt tattttcctc atcaactttg taaaccactc
    40021 gatgctgtct attcagcctg cgtgagtatc tgccttcatg tgtaggtctc agcttttcaa
    40081 aagactgcgt gggcatatat ggatcttcct tcaatgtctg aatcacttct tcaaattgtt
    40141 tctttaaatt cgtttgcttg atttttctta aatccacttt cgcagagttt ttgattgcga
    40201 ctgtatagtt actcatcaaa gagcttccca atcaatatca tcgatattcg taaagctact
    40261 gtcgtctttt tcgcggtctc gtactttatc taaagtacct gtttgctcaa gaaaaagggt
    40321 ttcttgaatc gctctccagt cttccagccc aatcaataca gcattgtttc cactacgatc
    40381 actaataatc tcgatttcag tatgattttc atttacctct ttcaataact gataaaaatt
    40441 tttacgagca gtgctaggat tcatcgttgt attagccatt gtctctcctc ctttttctga
    40501 tataattata gcgtacttga ttaagtacgt caaaaaaata caataaagca cttaaattca
    40561 gcaaactttt cctctttttt acatagagaa aaagacccct ttttagggag gatctttgtc
    40621 cacgctcact tcttactcct ttacgactag cggtagccaa acgtaaagta attggtaaca
    40681 acgtatcatc tttagcaaag gtcgaatcca aagcctgtga gtctatgcaa caacaccaat
    40741 acacgaaggt tcctcgttgc agtgtgactt gattgatatc ttgaataacc tgaggatacg
    40801 cacgaaagat ttgtaacggt tattcaaaac tgattttggc cgtcaagtct tctactgcct
    40861 caaaaatgat tgcctctttc gtctcctgtc attcatgttc actatcgatt tgatacttgc
    40921 ttagaccgtt gggtttgtga atcaccaatt aaaaatggca ctgagttatg gtaacaaaaa
    40981 atggtcactt gacgaggctg agattcctac ttatgaagac acatggcact taagagtcga
    41041 ggtaaaggaa gaagctgaaa atataggagg ctgtacatta tttggcaaag gattcggaaa
    41101 ttatgatcaa gacatcaaaa tcaaaatgtt ttacagctaa ataggaagaa taataacatt
    41161 tcatacgctg ttcccatctt taatggctta tagagggcac ctttagctga gaataagaca
    41221 aaacagaccc tctagcttca attaagagct agagggtctg tttttgattt taaaacttgg
    41281 tttatttctt caaatggtag ctataggtgc tgacaacgat attttcacgc caagacaatc
    41341 gcaatcttaa ccgcaaactt gtttggttat ggagaatatt ctgccagcgt ctgtttggta
    41401 caaattgagg aatcaacaca gtagttgtat agttttgttt cgctgcattt ttacttacga
    41461 tatctacata acggataatt ggattttcaa tcgagcgata agaagagtgg acgattgaaa
    41521 aacggacatc tgggaaatgc tttttaaatt ctgcttcgat ttctttttct ttttcgacat
    41581 cttcgtctag cgaaacatgc atcgcaacga cataatcacc gatcgaacgt gcgtaattca
    41641 acgcaccgat atttacccgt gtcacattcc cgactaatac aagaacggta ttcccgtcat
    41701 aatcatgtag ttttgcatca ttttctaaac gaagctgttc tgctactttt ttataatgat
    41761 catggatctt ataaaaaatg aacataacga ttggcataat gatgaagaaa ggccaaatgt
    41821 cccctaaccg atagacaaac aaaatcgcaa tgattgcata tgagatgaac gcacccgtaa
    41881 tattggcaat cgatttgctt aaccatcttt ttccttcttt tttccatttg acgaccatcc
    41941 ctgtttgaga taaagcaaat ggaatgaaca cgccgataga gtataacgga atcaagcgtt
    42001 ctgttgagcc atggaaaata aacagcaata cgatcgaacc tagcgctagt gtgataatcc
    42061 cattggaata gcctagtcga tccccacgat cttgatacat gtgaggcatg aatttatctt
    42121 ttgccagatt gtatgcaaga actggaaaag cagaaaaacc ggtgttagca gcaactgcta
    42181 aaatcagcgc tgtagcaaat tgaaggacgt aatacaaaat accatgtcca aagacagctt
    42241 taccaatttg agacaaaaca gtgacttctt tttcaggaac gatcccatac caatagttga
    42301 taaatgtgat ccccacgaag aagaaaccta agatcaatgc catcatcgtc aatgtagcag
    42361 cggcattttt tgctcttggc ttcttaaaga atggtacagc attactaatc gcctccaccc
    42421 cagttaaaga ggaagaacca gaagaaaatg ctcgtaaaat caaagcgata gaaacacccg
    42481 gcacagccgc tcctggaagt gcagtagctt gcaatggcac agcacctgtc acgatattat
    42541 ataaaccaac aacgatcagt aacgagatca caaggataaa tgtgtacacc ggaaataata
    42601 ggaacgatgc agattccctc agcccacgaa gattcagcat catcagtagc aaaacgattg
    42661 tcacagaaat agcgacttga tgaccgtaga gtgctggcac cgctgatgtg atcgcttcag
    42721 cacctgctga gacagatact gctactgtca acatgtagtc gatcaataaa gagccaccgg
    42781 aaatcaagcc agcattcttt ccaagattct cactgctgac gacatatgct cccccgccat
    42841 gaggataagc atggatgatt tgacgatatg atagcgttaa tgaaatcaat aaaataataa
    42901 caaaagcggc aatgggtagc gaataccaga tggctgcagc agacaaagcg actaaaacga
    42961 caacgatttg ttccgtacca tatgcaatag atgataacgc atcagacgac aataatgcca
    43021 aagcagcaaa tcgggttaat ttgtgctcat cattttcagc agattttaaa ggcttaccta
    43081 ctaacaacct ttttaagtaa tccaatttct aaactccttt acacacaatt ttacttcaag
    43141 ttttatagtc gaaaatttat agtaaacaag atgttatagt acgctcactg accttaaaag
    43201 tcaataaact tt
//
LOCUS       DARBEG010000015        41165 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000015 DARBEG010000000
VERSION     DARBEG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.015"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(451..1233)
                     /locus_tag="SCK20_000808"
     CDS             complement(451..1233)
                     /locus_tag="SCK20_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144895.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="SCK20_000809"
     CDS             complement(1298..2134)
                     /locus_tag="SCK20_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1144896.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="SCK20_000810"
     CDS             complement(2152..3561)
                     /locus_tag="SCK20_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1144897.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="SCK20_000811"
     CDS             complement(3575..5398)
                     /locus_tag="SCK20_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1144898.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="SCK20_000812"
     CDS             complement(6241..6567)
                     /locus_tag="SCK20_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144899.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="SCK20_000813"
     CDS             complement(6613..6975)
                     /locus_tag="SCK20_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG1144900.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="SCK20_000814"
     CDS             complement(7012..7227)
                     /locus_tag="SCK20_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1144901.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="SCK20_000815"
     CDS             7354..7689
                     /locus_tag="SCK20_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1144902.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="SCK20_000816"
     CDS             complement(7765..9762)
                     /locus_tag="SCK20_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144903.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="SCK20_000817"
     CDS             complement(9860..10135)
                     /locus_tag="SCK20_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1144904.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="SCK20_000818"
     CDS             10367..11101
                     /locus_tag="SCK20_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1144905.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="SCK20_000819"
     CDS             11140..12282
                     /locus_tag="SCK20_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1144906.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="SCK20_000820"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="SCK20_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG1144907.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /locus_tag="SCK20_000821"
     CDS             complement(13773..14243)
                     /locus_tag="SCK20_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG1144908.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="SCK20_000822"
     CDS             complement(14230..15465)
                     /locus_tag="SCK20_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1144909.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="SCK20_000823"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="SCK20_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG1144910.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="SCK20_000824"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="SCK20_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG1144911.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="SCK20_000825"
     CDS             complement(17731..18579)
                     /locus_tag="SCK20_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1144912.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="SCK20_000826"
     CDS             complement(18603..19289)
                     /locus_tag="SCK20_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144913.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="SCK20_000827"
     CDS             complement(19282..20316)
                     /locus_tag="SCK20_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1144914.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20379..20635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20711..21049)
                     /locus_tag="SCK20_000828"
     CDS             complement(20711..21049)
                     /locus_tag="SCK20_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG1144915.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="SCK20_000829"
     CDS             complement(21051..21407)
                     /locus_tag="SCK20_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG1144916.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="SCK20_000830"
     CDS             complement(21413..22600)
                     /locus_tag="SCK20_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1144917.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="SCK20_000831"
     CDS             complement(22791..23636)
                     /locus_tag="SCK20_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1144918.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="SCK20_000832"
     CDS             23870..24835
                     /locus_tag="SCK20_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG1144919.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25212..26255
                     /locus_tag="SCK20_000833"
     CDS             25212..26255
                     /locus_tag="SCK20_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG1144920.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26317..26781)
                     /locus_tag="SCK20_000834"
     CDS             complement(26317..26781)
                     /locus_tag="SCK20_000834"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG1144921.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27027..27974
                     /locus_tag="SCK20_000835"
     CDS             27027..27974
                     /locus_tag="SCK20_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1144922.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28185..30257)
                     /locus_tag="SCK20_000836"
     CDS             complement(28185..30257)
                     /locus_tag="SCK20_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1144923.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30190..30567)
                     /locus_tag="SCK20_000837"
     CDS             complement(30190..30567)
                     /locus_tag="SCK20_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG1144924.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30696..31274)
                     /locus_tag="SCK20_000838"
     CDS             complement(30696..31274)
                     /locus_tag="SCK20_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1144925.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31406..32698
                     /locus_tag="SCK20_000839"
     CDS             31406..32698
                     /locus_tag="SCK20_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1144926.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32758..33876)
                     /locus_tag="SCK20_000840"
     CDS             complement(32758..33876)
                     /locus_tag="SCK20_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG1144927.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="SCK20_000841"
     CDS             complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="SCK20_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG1144928.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="SCK20_000842"
     CDS             complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="SCK20_000842"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG1144929.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37082..37681
                     /locus_tag="SCK20_000843"
     CDS             37082..37681
                     /locus_tag="SCK20_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG1144930.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="SCK20_000844"
     CDS             complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="SCK20_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG1144931.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39376..39720)
                     /locus_tag="SCK20_000845"
     CDS             complement(39376..39720)
                     /locus_tag="SCK20_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG1144932.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39857..41002)
                     /locus_tag="SCK20_000846"
     CDS             complement(39857..41002)
                     /locus_tag="SCK20_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1144933.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11848 a         8652 c         7193 g        13472 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga
    25021 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa
    25081 caccgtttat tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg
    25141 tcaaaatact ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag
    25201 gaaccgatca aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc
    25261 gcgaaatctt acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg
    25321 ctatttatct gccacagtta ggtggagcga ctattgctat tttattgggt attttattag
    25381 gaaatacttt ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac
    25441 tattagaatt ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa
    25501 ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt
    25561 acttgattgg taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa
    25621 acgctgtttg tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg
    25681 aagaaaaagg acagatcatc acactagtca atctactggg tactgtgatg atgttgactc
    25741 tcccttttct tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg
    25801 gtggtacact ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg
    25861 ttgtcgaatt cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct
    25921 atctgtttga aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata
    25981 cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt
    26041 tggtcaacag cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca
    26101 gctggtttga gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca
    26161 tgaaagaagg tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga
    26221 tggctttact atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga
    26281 ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa
    26341 caacaacaat tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag
    26401 ttttccattt tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg
    26461 atttgcctgc ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt
    26521 atacgttcgg atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa
    26581 aggcgtacct ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata
    26641 ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga
    26701 aattggctga aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc
    26761 gataggtgtg tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt
    26821 tttctctatc caaaactact tgccaactaa taaaaaatgt tcttaaaagt ttgataaacc
    26881 gaggaaacat ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta
    26941 aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata
    27001 aacatacgat tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg
    27061 aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt
    27121 tcgaatattt gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc
    27181 tagcctcagc ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag
    27241 cggctctgta cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg
    27301 atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata
    27361 tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct
    27421 ttgcgaaaaa attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg
    27481 ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat
    27541 acaaccgatt ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt
    27601 atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact
    27661 actcgattga tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg
    27721 gcgctttgtc ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt
    27781 tgtttggtga tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg
    27841 gaatccaatt gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa
    27901 cgaaacagcg tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga
    27961 agcctcacca ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt
    28021 gacaaaagtc ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt
    28081 cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
    28141 ttcttgaagc tgaccacttc tatcacaacc tctttcatat cttattactt cttcattgct
    28201 gcttttagtg ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc
    28261 agcaagcgtc gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat
    28321 ttttctgtcg ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt
    28381 tccattttcc ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg
    28441 cggtagttgc aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg
    28501 cgcagattgg ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct
    28561 tgtttgcttg atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag
    28621 aaggttcctg cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct
    28681 gtcaaatctg gtgttactaa agagggattt accaatacta ataattgctt acctttagga
    28741 gatacttgca gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata
    28801 atctctgcac gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt
    28861 gcttctgaca atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg
    28921 attctcattc ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat
    28981 cctgcttgga cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta
    29041 acttttgttt ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc
    29101 atttgataga tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt
    29161 ggcaaaagtg catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg
    29221 atggctccgt ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat
    29281 cgttctttca tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa
    29341 gtaacgatct tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa
    29401 tagccatagc gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca
    29461 gattccgttt ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat
    29521 tgaacaagtt tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt
    29581 ttggcttgtt cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc
    29641 tcgattttct tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct
    29701 gataaattat ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag
    29761 tccgcttttg cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt
    29821 agttgcttga atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc
    29881 actggtttat tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt
    29941 ccggcatctg tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt
    30001 gcttttaatt gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt
    30061 ggcaacgtgt ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat
    30121 cctaataaat gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg
    30181 tgcttttgat tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct
    30241 agaattagtt gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt
    30301 caacaacgca agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc
    30361 atctccgtca ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt
    30421 ttgatggatt tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa
    30481 tgtttcttct tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc
    30541 cccgaacatg gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc
    30601 atcttagtca cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg
    30661 aacatcttaa aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta
    30721 ttgatcatcc aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct
    30781 aaaaaacgta aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact
    30841 ttttcttctt tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca
    30901 tggattccat ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac
    30961 actgcttgat ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc
    31021 tgaccacttt ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc
    31081 gctgttagcg ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc
    31141 ttgatatcca agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct
    31201 gctagttctc gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc
    31261 atgtttcctc tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga
    31321 aaaaagagac tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga
    31381 aaattttcag aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa
    31441 aagagaaact tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat
    31501 cggatttagt ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag
    31561 tacgtcacaa ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac
    31621 gcttatctct ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt
    31681 gcgtgcttca ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta
    31741 tcaattagtc gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac
    31801 tgcccttgtc gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc
    31861 aactggttct gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc
    31921 gtttggctat cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt
    31981 gagtctgttt ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc
    32041 caaacagatt ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac
    32101 gatgatcatc caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca
    32161 attgctgcat ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg
    32221 gattgccact ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga
    32281 aagaatactt gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt
    32341 cacgaatgtt tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct
    32401 gctccctgct gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat
    32461 cttcagttat aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc
    32521 cagtgtttcc agtgcatttg gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt
    32581 ggtcaacttc ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga
    32641 agaaaaagct tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc
    32701 gtaaatgaaa aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta
    32761 ggcctgtttt ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt
    32821 acaacagtaa cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag
    32881 tactcctgtt tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa
    32941 tttaatgaaa gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc
    33001 tacccattgt tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat
    33061 tttttcttta ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat
    33121 catcgtgtag tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca
    33181 gccaatacga atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt
    33241 ttcatagaca tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc
    33301 atctatcaat acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt
    33361 ccgatctagc aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc
    33421 aggtttgact aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa
    33481 atgaacaaag tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt
    33541 aggaagcatc gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt
    33601 agcaccagtc gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga
    33661 agcgacttct tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt
    33721 tttgcgtttt tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa
    33781 tgtcaatgct tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt
    33841 tagccattct tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga
    33901 tccagcctac agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca
    33961 ttactttttc ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc
    34021 aaaaaccagg aagataggca atactacata aaaggacata agaaaatcct gctaaaatga
    34081 tgcccatcaa ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt
    34141 cttttaaaga aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg
    34201 ctgtacataa agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga
    34261 atagattagt caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg
    34321 tttttcccca acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg
    34381 ggagcatcgt ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca
    34441 gcgctgctcc ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa
    34501 tagttgctaa ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga
    34561 taaacaataa acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg
    34621 cgcggttggc taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca
    34681 aacaaccttt aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat
    34741 cagatacgtt accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa
    34801 atattaaaaa taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc
    34861 tcgctccttc aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa
    34921 ataaaataga aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc
    34981 tcatcagcat cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac
    35041 cactgatgaa gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat
    35101 ctagatctgg acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa
    35161 tcccaacgat tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac
    35221 ttgtgatccc aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc
    35281 atttgctaat acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac
    35341 aaatcacttg caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac
    35401 tttattcata ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa
    35461 taaaaattaa ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt
    35521 tgttagccat agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt
    35581 ttcttgatat gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac
    35641 aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt
    35701 atcatccaat aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt
    35761 acacttgaat tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa
    35821 gcttgtggca tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc
    35881 tagatctttt cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag
    35941 tccttgacgt tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc
    36001 atcaagagtg accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa
    36061 gttcttttca ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc
    36121 tgcgcgttca gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt
    36181 ttttgaaagc tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc
    36241 tactccacga agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc
    36301 agtagctaca taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat
    36361 ttctttgttg cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa
    36421 atattcaaat acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc
    36481 gatttgagca gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt
    36541 ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt
    36601 taacaaaagt gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt
    36661 tttgctgttg tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag
    36721 ttgaaggtcg caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc
    36781 cttaattatc aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat
    36841 agtcaaccaa aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata
    36901 aaaagggcca gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc
    36961 aaaattgctt cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg
    37021 ttatgagttg ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata
    37081 catgaattac tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga
    37141 ctttgctccg cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg
    37201 gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc
    37261 gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat
    37321 ccacttgatc aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga
    37381 tcctaggctt actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa
    37441 attcgaccat caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac
    37501 tatccgcaat cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa
    37561 tgtaggcgta gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc
    37621 gttatctgag caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg
    37681 aaaaaactgc cctatatcaa gtattaaaca aataaagata attactcata tttttcattt
    37741 gagtaggctt attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg
    37801 tcacaactcc attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt
    37861 ctgtaattct ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa
    37921 taaaatcccg attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg
    37981 tcctgcaaat aataatccaa ttggacctgt caggttcatc aaagagttga agttccctaa
    38041 gacacgacct aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt
    38101 gaaatatggc gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg
    38161 actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat
    38221 aataataggt ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa
    38281 gagcattccg actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa
    38341 gtaatctagc gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa
    38401 tgcaccattg atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa
    38461 ttttgcatct ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat
    38521 cttcacaaaa atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag
    38581 gtaattgaca ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt
    38641 cgctgattgg accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat
    38701 tgtcggcata atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa
    38761 caaagacaca aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa
    38821 aataagcgcg aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca
    38881 gcgatccact agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa
    38941 agtagcaaaa ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc
    39001 atattggacg accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata
    39061 atttctcttc caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt
    39121 ctttttcatt ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa
    39181 ataccttttt ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa
    39241 atcagcaaaa tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa
    39301 tttgcgtcgt ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct
    39361 ttttatatcc acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat
    39421 cagaaaataa aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc
    39481 tttcaatcca tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt
    39541 gatttttaat ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg
    39601 ataattgcct ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc
    39661 aggatgcaaa cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat
    39721 gattttcacc tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact
    39781 aggtttttca gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata
    39841 agaatgactt cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga
    39901 tgtcgtcttc ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg
    39961 tatcttctcc atacattgct tccaatacgt gtgaaggctc gatatttcct gctgtacaag
    40021 ctgaaccagt agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg
    40081 cttcgccaat taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta
    40141 cttgataagg gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat
    40201 atttgttgtt acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac
    40261 cccagatccc tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta
    40321 aaagcaatgc agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc
    40381 cattgatttt atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg
    40441 tttggtttcc gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa
    40501 gttcaccgat ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag
    40561 agaccaagat cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt
    40621 tttcatctac aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat
    40681 taagaacagc aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg
    40741 agaacgctgt ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga
    40801 tctcgtgagg ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt
    40861 catgcgcgtg gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga
    40921 ttgccgtcat cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat
    40981 aaatagtttt cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa
    41041 tagcgaacaa accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa
    41101 aaaggagctg tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac
    41161 ttctt
//
LOCUS       DARBEG010000016        40931 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000016 DARBEG010000000
VERSION     DARBEG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.016"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..1189)
                     /locus_tag="SCK20_000847"
     CDS             complement(38..1189)
                     /locus_tag="SCK20_000847"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG1144934.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="SCK20_000848"
     CDS             complement(1202..1639)
                     /locus_tag="SCK20_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1144935.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="SCK20_000849"
     CDS             complement(1654..3420)
                     /locus_tag="SCK20_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG1144936.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="SCK20_000850"
     CDS             3684..4361
                     /locus_tag="SCK20_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144937.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="SCK20_000851"
     CDS             4364..5491
                     /locus_tag="SCK20_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1144938.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="SCK20_000852"
     CDS             complement(5691..6131)
                     /locus_tag="SCK20_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1144939.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="SCK20_000853"
     CDS             complement(6142..8175)
                     /locus_tag="SCK20_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1144940.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="SCK20_000854"
     CDS             complement(8177..9880)
                     /locus_tag="SCK20_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1144941.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCK20_000855"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCK20_000855"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG1144942.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="SCK20_000856"
     CDS             complement(10963..11988)
                     /locus_tag="SCK20_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1144943.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="SCK20_000857"
     CDS             complement(12197..13066)
                     /locus_tag="SCK20_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG1144944.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="SCK20_000858"
     CDS             complement(13525..15417)
                     /locus_tag="SCK20_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144945.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="SCK20_000859"
     CDS             complement(15414..17141)
                     /locus_tag="SCK20_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144946.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="SCK20_000860"
     CDS             complement(17157..17807)
                     /locus_tag="SCK20_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1144947.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="SCK20_000861"
     CDS             18011..18307
                     /locus_tag="SCK20_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1144948.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="SCK20_000862"
     CDS             18479..19099
                     /locus_tag="SCK20_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG1144949.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="SCK20_000863"
     CDS             complement(19191..20357)
                     /locus_tag="SCK20_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1144950.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="SCK20_000864"
     CDS             complement(20369..20722)
                     /locus_tag="SCK20_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1144951.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="SCK20_000865"
     CDS             20850..21872
                     /locus_tag="SCK20_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG1144952.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="SCK20_000866"
     CDS             21891..22052
                     /locus_tag="SCK20_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144953.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="SCK20_000867"
     CDS             complement(22311..22745)
                     /locus_tag="SCK20_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144954.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="SCK20_000868"
     CDS             complement(22855..24714)
                     /locus_tag="SCK20_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1144955.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="SCK20_000869"
     CDS             complement(24832..25992)
                     /locus_tag="SCK20_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG1144956.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCK20_000870"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCK20_000870"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG1144957.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="SCK20_000871"
     CDS             complement(27739..28437)
                     /locus_tag="SCK20_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1144958.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="SCK20_000872"
     CDS             complement(28394..30055)
                     /locus_tag="SCK20_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG1144959.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="SCK20_000873"
     CDS             30273..31673
                     /locus_tag="SCK20_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG1144960.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="SCK20_000874"
     CDS             complement(31925..33004)
                     /locus_tag="SCK20_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1144961.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="SCK20_000875"
     CDS             complement(33090..34049)
                     /locus_tag="SCK20_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1144962.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="SCK20_000876"
     CDS             complement(34061..35572)
                     /locus_tag="SCK20_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1144963.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="SCK20_000877"
     CDS             complement(35594..36157)
                     /locus_tag="SCK20_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG1144964.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="SCK20_000878"
     CDS             complement(36163..37026)
                     /locus_tag="SCK20_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1144965.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="SCK20_000879"
     CDS             complement(37040..37936)
                     /locus_tag="SCK20_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1144966.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="SCK20_000880"
     CDS             complement(38111..39400)
                     /locus_tag="SCK20_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1144967.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="SCK20_000881"
     CDS             complement(39372..40889)
                     /locus_tag="SCK20_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1144968.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11794 a         8582 c         7090 g        13465 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt t
//
LOCUS       DARBEG010000017        37214 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000017 DARBEG010000000
VERSION     DARBEG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.017"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            59..808
                     /locus_tag="SCK20_000882"
     CDS             59..808
                     /locus_tag="SCK20_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1144969.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="SCK20_000883"
     CDS             complement(1232..1402)
                     /locus_tag="SCK20_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1144970.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="SCK20_000884"
     CDS             complement(1596..1775)
                     /locus_tag="SCK20_000884"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144971.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="SCK20_000885"
     CDS             2162..3274
                     /locus_tag="SCK20_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1144972.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="SCK20_000886"
     CDS             complement(3352..3645)
                     /locus_tag="SCK20_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG1144973.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="SCK20_000887"
     CDS             3813..4127
                     /locus_tag="SCK20_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG1144974.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="SCK20_000888"
     CDS             4148..6127
                     /locus_tag="SCK20_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1144975.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="SCK20_000889"
     CDS             6140..6466
                     /locus_tag="SCK20_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG1144976.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="SCK20_000890"
     CDS             6482..7000
                     /locus_tag="SCK20_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1144977.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="SCK20_000891"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="SCK20_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1144978.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="SCK20_000892"
     CDS             complement(8436..9941)
                     /locus_tag="SCK20_000892"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG1144979.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="SCK20_000893"
     CDS             complement(10033..10623)
                     /locus_tag="SCK20_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1144980.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="SCK20_000894"
     CDS             10802..12448
                     /locus_tag="SCK20_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1144981.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="SCK20_000895"
     CDS             12497..13216
                     /locus_tag="SCK20_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1144982.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="SCK20_000896"
     CDS             13695..14642
                     /locus_tag="SCK20_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1144983.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="SCK20_000897"
     CDS             14654..15574
                     /locus_tag="SCK20_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1144984.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="SCK20_000898"
     CDS             15615..17072
                     /locus_tag="SCK20_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1144985.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="SCK20_000899"
     CDS             17417..18031
                     /locus_tag="SCK20_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1144986.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="SCK20_000900"
     CDS             18024..19748
                     /locus_tag="SCK20_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1144987.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="SCK20_000901"
     CDS             19745..21196
                     /locus_tag="SCK20_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1144988.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="SCK20_000902"
     CDS             complement(21669..23822)
                     /locus_tag="SCK20_000902"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG1144989.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="SCK20_000903"
     CDS             complement(23824..24870)
                     /locus_tag="SCK20_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1144990.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="SCK20_000904"
     CDS             25020..26309
                     /locus_tag="SCK20_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG1144991.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="SCK20_000905"
     CDS             26322..29024
                     /locus_tag="SCK20_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1144992.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29009..29866
                     /locus_tag="SCK20_000906"
     CDS             29009..29866
                     /locus_tag="SCK20_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1144993.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29937..30836)
                     /locus_tag="SCK20_000907"
     CDS             complement(29937..30836)
                     /locus_tag="SCK20_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1144994.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31005..33176
                     /locus_tag="SCK20_000908"
     CDS             31005..33176
                     /locus_tag="SCK20_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1144995.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33244..33585
                     /locus_tag="SCK20_000909"
     CDS             33244..33585
                     /locus_tag="SCK20_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1144996.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33861..35615)
                     /locus_tag="SCK20_000910"
     CDS             complement(33861..35615)
                     /locus_tag="SCK20_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1144997.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35626..36462)
                     /locus_tag="SCK20_000911"
     CDS             complement(35626..36462)
                     /locus_tag="SCK20_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1144998.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36464..37147)
                     /locus_tag="SCK20_000912"
     CDS             complement(36464..37147)
                     /locus_tag="SCK20_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG1144999.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12005 a         6542 c         7633 g        11034 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaggg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctctgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgagtcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggtttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atgcttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggcc ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    29101 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    29161 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    29221 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    29281 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    29341 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    29401 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    29461 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    29521 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    29581 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    29641 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    29701 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    29761 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    29821 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    29881 caaaaagaac agagtcttat cattatctgc tgataagact ctgttctttt ttatttctat
    29941 tcccataatc caggtaaagc tttaccgact attttttctt gagtaaatgg atggatcatg
    30001 tgcagttcta atgcatggag catcagtcgt tcagctgttc tattttggta gagagggtct
    30061 ccaacgatcg gatgtccctt atgagctaaa tgaactctga tctggtgtgt acgtccggtt
    30121 tccaaaacac aatagacttg tgtttgttca gaaagtactt tgttgacagt gacatgtgtc
    30181 aaggcgctct gaccattttt atcatctacg accctttttc tccgatcatg gcgatctcgt
    30241 ccaattttat tggtgatggt catatctttg gcaatttctc cccatacgat ggcttgatat
    30301 ctccgataga tcattttttg ttccaacatt cttccaagta tcggcaagac aaatggattt
    30361 tttgcaaata aaacggcacc gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt
    30421 tgctgtttag tgtcgagata agcagctaaa tgattgagta aggtactgtt ttctgtcggt
    30481 tcatttggat gagtcttcat tcctgcaggc ttatccacta taatcaaatg ctcatcttca
    30541 tataaaacac ggatgtgctt cgcatctcct aattgaactt cttgataatt ataatctgtt
    30601 tcttcgaata acaatgtgat ctggtcattg ccctttactt catgatggaa caaagcaggt
    30661 tgttgattga tccaaacgtt tttacgtata cgcaaaaagt gtcgaacctt tctaggaact
    30721 aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg
    30781 atcgtgattt tcacaagtgt actcctttca gtgcaattgc agactaattg taccattttt
    30841 aaaacatatt taagaaaaaa cttacatttt tctgttcatt cttatgttac tctacttgca
    30901 ctaacgtaaa aaaacatatt gaactacaac ttaagcagga agaaaacact ctttcactat
    30961 ggtaaaataa gacgattgaa tcaaatagac aggagaagaa gcaaatggat tttcaaagca
    31021 ttttagaaaa gataaaagct gctttgatca gtttttggcg ttggatcaga ccatatctcg
    31081 gacaattcca tcgatggcga aagcgcatct ggaaaaaata tcatgtgaac aaattgattt
    31141 tgctgattgg tttgatctgt gtcttagtga caagtatcta cctctttatt ttagcaaaac
    31201 aagcaaatgt cgaaacgtta aaatcaggat taagccaatc gacagtcatt tatgataaga
    31261 aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga
    31321 tttctcccta tatccaagat gcagtaatct cgacagaaga taggaatttt tacgagcatc
    31381 acgggtttga tatcaaaggg attgcccgtg cagcagtccg aatggtcttg tctggcggaa
    31441 catcaggggg cggaggaagt acgatcactc agcagcttgc caaaaatgcg tatctgacat
    31501 tagaccaaac gtttgatcga aaagcgaagg aattattttt ggcaatcgag atcgaaaaga
    31561 agtacagcaa agaagaaatc ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg
    31621 tatggggtgt ccaagacgct tccagaaagt attttggagt agatgcaacg aatgttacgc
    31681 tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg
    31741 atcacccaaa aaatgcgaat gatcggaaaa acacagtact aagtgtaatg gaagaaaacg
    31801 gaaaaatcac aaaagaacaa gaacaagcag aaagccagac agatatcagc gcgtatctta
    31861 atgacacata cgataattca gattctggtt atcgttatcc ttattatttt gatgcagtga
    31921 tcgatgaagc agttaacgag tatggtcttg atgaagaaga tgtcatgaat aaaggctata
    31981 aaatctatac agctttagat caggattatc aaaagcaatt agaagctact tatcaaaaca
    32041 gtgcattatt cccagcaaat gcttcagatg gtgccatggt ccaatctgct tcagtagctt
    32101 tagatccgaa tacaggtggt gtacaagcac tagtaggacg aagaggagat catgtcttta
    32161 gaggattcag ctttgctaca cagatgcgtc gttcaccagg atcaacgatc aaaccattaa
    32221 gcgtatatac accagcactg gaagctggat acaagccttc cagtatcttg aaagacgaac
    32281 cgcaaagtta ttacgatgcg cataattttg acgggacata ccaaggagaa gtaccgatgt
    32341 atcaagctgt tgcacaaagt ttgaatttgc ctgctgtatg gcttttgcat gagatcggtt
    32401 tacaaaaagg atatgataaa gcagaagaat tcggcctgcc tttagcaaaa tctgataagt
    32461 attatggctt agcccttggt ggactggaaa aaggagtatc tccattgatc atggcgggtg
    32521 cttacagtgc atttgccaat gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg
    32581 ttgattcgac tggagcggtg atcgtcgata acacaaacaa aaaaccaaaa caggtcatat
    32641 caaaggaaac agccgatgaa atgaccagca tgcttcttgg tacattttca aatggaacag
    32701 cggctgctgc gaatcctgca ggttatacga tagcagggaa aacaggaacc acagaaacta
    32761 atttcgatgc cacaaaagca aatgaccaat ggatgatcgg ttacacgcca gacgtagtga
    32821 tttcgacatg gatgggttac gaagaatcca gcaagctgca ctatttggaa gggacaagtg
    32881 gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg catccttccg tatacttcaa
    32941 aaacaggatt tagtgtagca gatgcctatg ccacaggagg acaagtggta gctgcagatc
    33001 aagttggaaa cacaggaaat tctaatggag agtctggaaa ttggcaagat aacttgaatc
    33061 aatatggtga acaagcaaaa gaagggctga aaaacttcgg cgatatggtg aaagaaggcg
    33121 ttcagggaat tggtgaagcg gctaaagacc tctggcggaa atatcaagga gagtagcccc
    33181 tttgaaaacg aataacccca tgttacaata gagagtaact actaaaagaa aagagggcat
    33241 aacatgtcaa acatctacga tagcgcgaat caaatcgaac gtgagatccg tgaattacca
    33301 gaattcaaag cgttgaaaga agcgtacgag aaagtacaag caaacgaaga agcacacaaa
    33361 ctgttcaaag acttccaagc gatgcaaatc gaattacaag aaaaacaaat gagtggacaa
    33421 gagttcagcg acgaagatgc aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa
    33481 gaagtaatca atgacttgat gcaaaaagag caaggcttca gtacaatcat caatgacttg
    33541 aaccgtatca tcatgacacc agttcgcgac ctttacagcg aataaaacta ttttatgaaa
    33601 caacagaggg tgtgaaagag acgttcggcc ttgagcgatg acaaaattgc ttcttattgc
    33661 cgataggctg tctcttgaac accatttacc aagaggtcgt gagcctaccg tttaactctg
    33721 agttacaagg aacaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    33781 accgaggagt tggcaggaga acaccgttta ttcagaaata ggagctacga caaaagtctt
    33841 gtcatagctc ctattttttg tcattcattt ttcaagcggt agaaatagcc tttttgagct
    33901 aacagttttt cgtaagtacc tatttcaacg atttttcctt catcgatcac aacgatttta
    33961 tctgcctctt tgatcgttga aagccgatga gccacaataa aagtagtggg tgtttctgtc
    34021 aggtaattca gggcttgctg gatttttttc tctgactggt tatctaaagc tgaggttgct
    34081 tcatctagca aaatgatttt tggctgacgt atcaacgctc gtgcaatgga gatacattgc
    34141 ttttggccac cagacagctt gttgccatgt tcgcctacta aggtatccag accatcagga
    34201 agctggtcaa tcagtgaaga aagttgcgca gcttcgatga cttcatctaa acgttctttt
    34261 gatacgtttt tcaagccata agtgatgttc tctttgatcg aagcggagaa caacaaagtg
    34321 gtttgaggaa caacggctaa ataatttcgc acacttcgca tatccagttc atccaaaggc
    34381 tgatcatcta gaaaaatttt tcctgaagtc ggctggataa atccaatcaa taagttaagg
    34441 agtgtagatt ttccagagcc agatggccca acaaaagcaa ttgtttctcc agctttcacc
    34501 tctagagaaa aatgatcgat aatcggtttg agtgagtccg gataaagaaa agaaacatcc
    34561 tcaaaacgaa aatttccatc caaatgttgg atttttttct taccgtgata agtttctaca
    34621 tcgccagcaa tcaacagttc gccaatagaa gtgatagatt ccatgccttt tgaaatcgta
    34681 ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg tcgtgaaata cgtctgatac
    34741 atcacgacat cgccgggttg gatgcgccct tgtaaagcta atatcccagt gaaaatcaaa
    34801 caaatcagct gaaatatctg aaaactagcc cagctgactg atccgaaaac agattgcagg
    34861 atatctaagt ggaatccttt ttcatagatt tgagtaaaat gcttatttaa gcgagttgtt
    34921 tcgtgttttt ctaacccatg agctcgagca atcggtgcta attcgatcac ttcgatcact
    34981 tttgcagaag tctcttccat ttcttgcctg aagtctgagt tggactgtct gatcctttta
    35041 cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca ataagaaaaa catgaaaaca
    35101 acaggacttt tataaagcgt gatacctaaa gctacgaata aattgacagc gatatttaat
    35161 ccgctgacaa atacttgaga ggacaacgtt tggatagctt ctacatcacg gatgatcttt
    35221 gactggattc gtccagattg caattctttt tgataaggaa tagatagttc ttggattttc
    35281 ttgataaggg tcgctcttag tccagcttct acttgacgga tcgaagtgct atggtatttt
    35341 acatgaaaat aattcgtcaa tatattttga ccaatcagac aagccatcaa aaaagcgttg
    35401 aaataaatgg cctgcaactg agaagaatct ttcgatgaag ctgcattgat tacattggca
    35461 atagcgatcg gcattaacca agatggaaga tgtttgataa taaaaaagag tcctgacatg
    35521 gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg tctgaaaagc atttgcatga
    35581 tatttttgat aacttttctg tattcgttcc tccataatct tctccttaac aatcttggta
    35641 aataaactgc aagtgttcta ttgcttcagg aacataaatc tcaggagtag cttcaagagt
    35701 ggcgtacatt agaggacgct catatttgat ataatgcagt accgggcgga aatctaaaca
    35761 gcccttgcca acaggtacca tgcggatctt tccattttcc atgatatagt ctttgagatg
    35821 aagcgctgta atataaccat gtaaattgtc caaggcacct cggatcactt tttcctgatc
    35881 agcatgattt tctttatgca tcaaattcgc acaatctaaa atgattttta aatttggtga
    35941 agctacttca tcgattaaac gtttagctaa ctgatagtta tagattggat gattgatccc
    36001 tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga gcgactaatt caatgataga
    36061 atttctggcg atctgataag cttcttcagt aaaattttct tttgtgtaac cgacagagac
    36121 actaccggtt tccgtagcaa tcatcttggc ttgataggca gagcacagag caatttgctg
    36181 tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt ttgctggata ggttgatata
    36241 gcatcctaaa atggaaagtt cgatattgtt tttttggaag tattcaccaa aataggcagc
    36301 actcccaagt gtcatgtcag ttaatttaag ttgttcagga aatgcttttt gtggtgcaaa
    36361 ttggatatgg gacaagtgaa gctgtgatac tttttgaacg atttcttcac gatttcgaac
    36421 atctaaatcg tgggcacgga tgcctaaatt taatggaaac atctcaaact cctttttgta
    36481 aataattagc aagaggcaga cgggattctt ttatcaattc aacaattatt cgggcaactt
    36541 tatcagcgcc ttctggacta aagtgggtat tgtcttttag tccttctggg taatttttac
    36601 aagtatcagg cataagatgc aggtagaatt tttttgtctc ttcttttgag aaggtctgga
    36661 acaattcttg tgatcttgag aacacatcta aaagaggaat atggtgtttc tcagcaaatg
    36721 cacgcatcgc ttttggatag tctccaagta tatctggatt caatgttccg ttttctaagt
    36781 aatctcgtcg cgtcacagat gtcagtaaga ccatttggac gttcgctttt tttgcgcatt
    36841 caaaaaatat ctgcagattt tttaaatagc taccgaaggg aagcgtacct ctttcttctt
    36901 ggattttctg gtcgttatgt ccaaattgta caaataagaa atcattcggc tgaaactgtg
    36961 aagctagacg ggctaacctt ccttcctgga taaatgattt ggtgctgcgt ccattctttg
    37021 cttggttatt gatctggatc tcgggtgaaa aataagcaga gattttttct ccccaacctg
    37081 tctcgggacg cttgttttca tctttttctg ccgcagtaga atcaccggca atatggatag
    37141 taggcatgtt atctcttctt tcataaaggt ttataggcaa tgaagcagaa cagcaagaga
    37201 agaagacgga gcaa
//
LOCUS       DARBEG010000018        35583 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000018 DARBEG010000000
VERSION     DARBEG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.018"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            71..1141
                     /locus_tag="SCK20_000913"
     CDS             71..1141
                     /locus_tag="SCK20_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145000.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            1156..1824
                     /locus_tag="SCK20_000914"
     CDS             1156..1824
                     /locus_tag="SCK20_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145001.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            1844..2422
                     /locus_tag="SCK20_000915"
     CDS             1844..2422
                     /locus_tag="SCK20_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1145002.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            2825..3736
                     /locus_tag="SCK20_000916"
     CDS             2825..3736
                     /locus_tag="SCK20_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1145003.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3992..4909)
                     /locus_tag="SCK20_000917"
     CDS             complement(3992..4909)
                     /locus_tag="SCK20_000917"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG1145004.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            5111..5548
                     /gene="zapA"
                     /locus_tag="SCK20_000918"
     CDS             5111..5548
                     /gene="zapA"
                     /locus_tag="SCK20_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG1145005.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            5606..6153
                     /locus_tag="SCK20_000919"
                     /pseudo
     CDS             5606..6153
                     /locus_tag="SCK20_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            6268..8628
                     /locus_tag="SCK20_000920"
     CDS             6268..8628
                     /locus_tag="SCK20_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1145006.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            8715..9029
                     /gene="trxA"
                     /locus_tag="SCK20_000921"
     CDS             8715..9029
                     /gene="trxA"
                     /locus_tag="SCK20_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG1145007.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            9211..10989
                     /gene="uvrC"
                     /locus_tag="SCK20_000922"
     CDS             9211..10989
                     /gene="uvrC"
                     /locus_tag="SCK20_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG1145008.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            11134..12033
                     /locus_tag="SCK20_000923"
     CDS             11134..12033
                     /locus_tag="SCK20_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145009.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            12026..13288
                     /locus_tag="SCK20_000924"
     CDS             12026..13288
                     /locus_tag="SCK20_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145010.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            13451..15349
                     /locus_tag="SCK20_000925"
     CDS             13451..15349
                     /locus_tag="SCK20_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG1145011.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(15648..16367)
                     /locus_tag="SCK20_000926"
     CDS             complement(15648..16367)
                     /locus_tag="SCK20_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG1145012.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            16704..17036
                     /locus_tag="SCK20_000927"
     CDS             16704..17036
                     /locus_tag="SCK20_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145013.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            17023..19026
                     /locus_tag="SCK20_000928"
     CDS             17023..19026
                     /locus_tag="SCK20_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG1145014.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            19045..19515
                     /locus_tag="SCK20_000929"
     CDS             19045..19515
                     /locus_tag="SCK20_000929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG1145015.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            19536..20120
                     /locus_tag="SCK20_000930"
     CDS             19536..20120
                     /locus_tag="SCK20_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG1145016.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            20135..21121
                     /locus_tag="SCK20_000931"
     CDS             20135..21121
                     /locus_tag="SCK20_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG1145017.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            21111..21422
                     /locus_tag="SCK20_000932"
     CDS             21111..21422
                     /locus_tag="SCK20_000932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG1145018.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            21444..23225
                     /locus_tag="SCK20_000933"
     CDS             21444..23225
                     /locus_tag="SCK20_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG1145019.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            23218..24594
                     /locus_tag="SCK20_000934"
     CDS             23218..24594
                     /locus_tag="SCK20_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG1145020.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            24601..25236
                     /locus_tag="SCK20_000935"
     CDS             24601..25236
                     /locus_tag="SCK20_000935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG1145021.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            25266..26621
                     /locus_tag="SCK20_000936"
     CDS             25266..26621
                     /locus_tag="SCK20_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG1145022.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(26813..28207)
                     /locus_tag="SCK20_000937"
     CDS             complement(26813..28207)
                     /locus_tag="SCK20_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1145023.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            28299..29270
                     /locus_tag="SCK20_000938"
     CDS             28299..29270
                     /locus_tag="SCK20_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG1145024.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            29267..30049
                     /locus_tag="SCK20_000939"
     CDS             29267..30049
                     /locus_tag="SCK20_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG1145025.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(30218..30589)
                     /locus_tag="SCK20_000940"
     CDS             complement(30218..30589)
                     /locus_tag="SCK20_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1145026.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            30723..31166
                     /locus_tag="SCK20_000941"
     CDS             30723..31166
                     /locus_tag="SCK20_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG1145027.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(31209..32018)
                     /gene="yidA"
                     /locus_tag="SCK20_000942"
     CDS             complement(31209..32018)
                     /gene="yidA"
                     /locus_tag="SCK20_000942"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG1145028.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(32037..33404)
                     /locus_tag="SCK20_000943"
     CDS             complement(32037..33404)
                     /locus_tag="SCK20_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1145029.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(33388..34218)
                     /locus_tag="SCK20_000944"
     CDS             complement(33388..34218)
                     /locus_tag="SCK20_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG1145030.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            34381..34818
                     /locus_tag="SCK20_000945"
     CDS             34381..34818
                     /locus_tag="SCK20_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG1145031.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            34894..35514
                     /locus_tag="SCK20_000946"
     CDS             34894..35514
                     /locus_tag="SCK20_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG1145032.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        11622 a         6053 c         7652 g        10256 t
ORIGIN      
        1 aatattcgcc aaaaatttat caagtgataa aattgttgta taaaatgatt atttcaggag
       61 gaggagttaa atgtttttag cattaaatga aattatgcat tcaaagttac gttatgcgtt
      121 agttgcaggg gtgatgtttt tgatcgccta cctggtattt tttttgactg ggttagctta
      181 tggcttagct caagataatc gaacagcagt cgacaagtgg gaggcagatt cgattgtgct
      241 ttcgaaagat gcaaattcaa atctagggat gtccatgatt actaagaaga tagcagaaga
      301 agtcgaaggt gggaaagtgg cgtatcttgc ccagacacct ggagtcgtga ctagtaaaga
      361 cagtacagaa gaaggaaaaa tcaatgttag cttcttcggt atcgataaaa atcaattcat
      421 catgcccaat cttgttgaag gaaaagcttt tgataacgat gatgaggcag taggcgatat
      481 tagtttgaaa gaagaatatg gtctagccgt aggagataca gttaagttat ctggtagtga
      541 caagacgttt aaactaactg gctttacgga tcatgccaaa ttcaatgtct caccagttct
      601 ctatacaacc atcaatgctt accaagagat ccgcttcgaa aaagaagata caagtgaaaa
      661 tgcacggatt aacgcaatag ttgtcagagg taaaatcagt aatttacctg aagatttaga
      721 acagatcaaa atttctaaat tcatcaatga attacctgga tataatgcgc aagtattgac
      781 ttttggattt atgatcggat ttttgatcgt tattgcggct attgtgatcg ggattttcat
      841 ttatgtactg acgatgcaaa agattaatat ctttggtgta atgaaagcac aagggatcac
      901 aggaggattt attgcgcgtt ctgtagtggc acaaacattc attttatcat ttgttggtat
      961 tcttcttggt ctattaggaa cagtgggaac atcccttgtt ctacctgatg cggttccatt
     1021 ccaaagcaat tggctgtttt ttggcgtcat tagcttgttg atgctggtag tagctgtgtt
     1081 aggcgcacta ttttctgttc ggacaatcgt aaaaatcgat ccgttgaagg caattggata
     1141 agaaggagga agagcatgaa agcaatagaa tttaaagaag tagaaaaaag tttccaagat
     1201 ggcgaccaag tagttaaagc attgaaagaa acaaccatga gtatcgataa aggggaattt
     1261 gttgcaatca tcggtccttc tggttcaggt aaaagtactt ttttgacttt ggcaggcgga
     1321 ttgcagacgc ctagcagtgg ctctgtattt atcaatgatc aagaattcag taatcaaaaa
     1381 gaaagtgaac gcgttaaact acgttttaaa gaaattggct ttattttgca agcttccaat
     1441 ctggttcctt ttctgacagt aaaaaagcag ttattattag tagataagat caaacatgaa
     1501 catcgtcaaa aagaagcaaa agaattattt gagcagcttg gcgtggataa gttgatcaac
     1561 aagtatccag aagaactatc tggtggagaa cggcaaaggg tcgcaatcgc tcgtgcattg
     1621 tacaatgatc cgtccattat tctagcagat gaaccaactg ctagtttgga ttcagaaaag
     1681 gcacgagaag tagtcgatat cttggcaaaa gaatcaaaag acaaaaataa agcaacgatc
     1741 atggttactc acgatacacg gctgattgac aaatgcgata gaatatttat gatggaagat
     1801 ggtgtattga aagaaaaaga ataaccaaaa agcaggtgga aaaatggtcc atcgatacaa
     1861 caaagataca cagcaaaaaa ttttgaaaga agcgactgac ttgtttatgt ccaagggata
     1921 tctcggcact tcaacaagag agatcgcaca aaaagttggg atcacccaac ccaatctcta
     1981 tcattatttc ggtgataaag aaaaactata tacggcagtt ttagaaaacc acttaaaaga
     2041 tgttggaaaa gctttgagag aaatcgttca aacaagcgaa acgggatttc aagtaacctt
     2101 gacaaaaatg gcgcagtttt tgattgagac acatctggtc gatttgtttc tgatgcttca
     2161 cgatcttgaa agcaatcttt caaaggaaac aagagatcat cttttctttc tatggaagaa
     2221 aaattatcgt gaaccttttg aagaaatctt ttcagcaaac caatctgtgc taagaaatgg
     2281 gatttcaaaa gaaatcgcag caaggcattt tttcttagtg ttagcacctt acatcactcg
     2341 gtcgaaaaga cctgtggaag aaccgctaaa tgtggcgcaa ttgatcgatt tatatttgaa
     2401 tggtgtatta ggagaagcgt aaagctgtta tatagggaga aaaatagtta ggtgaagaag
     2461 caagatatga cgaagatcgt tatatcttgt tttttttata taggtagttt ttcataaata
     2521 caaatttgtt attctctcat gattatcagg gtatattttt atttattatg tcggtgttcc
     2581 aggaagtgtt ttaatttaat aacgaagaaa ttaattgttt gtagaatgaa ggaaactatt
     2641 tattgggtag atgaatcacc acgaatagtc aaaaggagat ttcaggatat gccaaaaatg
     2701 aaaaaaatca ttttagcagt aatgttattt tctacggtta tatcttttac agcccctttt
     2761 ttgcgtactt aagctgattt taagttttga cccttagtct ggatctacga ggtgagagtc
     2821 aagaatgaaa aaaataattg gagcttttgt atttgcaatc attatcatta ttatctgttt
     2881 tcaaatagat gtttttggtc aaaaaaagat agttggacaa acggtcagca gtacgaacag
     2941 tacggttaca aagacaaagg aatcatttga tgatacagaa caagagacat taatcaaaat
     3001 cggaaaaaat gaccgtacac tatatacaga tgactcgcta actaccccac ttgcaactat
     3061 aaacggcgga gagctggcgg agtttctttc tgaaacaaaa aatgcttatc agatcaagac
     3121 aaatgatggc tatactggat atttagccct cgaagatgga accaaagtca caaagaatat
     3181 acaaacaaaa ccggaagagc tatctgatgc agtgattgta ttagatcctg ggcatggcgg
     3241 aaatgatact ggggcattaa gcaatgatga acaaacagaa gaaaaagata taacattaag
     3301 tacagccata aaagtaaaaa aggcgctaga ggatgctgga gcaacggttt atttgaccca
     3361 tactacagat gaattagtac agcttggaga tatatgcgac tacagtgaag aaaaaaaggc
     3421 agatgtcttt atcagtttac acgcagactc tacggaatat gcgaatgaag caactggaat
     3481 cacgacatat tattactatg gacaagagga aacattagca cagacaattg ctgactcctt
     3541 cactgatctg cctctggatt caagaggaat ctccactggg aactatcaag ttcttcgaga
     3601 aaatcttcaa ccttctattt taatcgagat gggttatatg aataatgata gtgatttaaa
     3661 ggaattagta acagacagtt atcagcagca gattgctgca agtttgacac aagcattgac
     3721 cacttatttt cagtaataaa taagcccagc caaacaaaaa tagctcctca tatttttcgt
     3781 ttggccgggc ttattatcgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg
     3841 agcctgccgt ttaactccgc gtcacaagga gcaatttcaa aaaacagctt ctcatgtttg
     3901 atgactattg tgacttgtga ccgaggagtt ggcatgagaa caccgtttat tcggaacaag
     3961 ggactgtgac atgacttttg tcacagttcc attatttttt tagcagcttc aaagcttttt
     4021 ctgtattggc aaaatgaagt ttggcatatt tttcaagcat aggcatgccg cctttctcca
     4081 atagttttgc cgctacttgg tcagatttcg ttccagctcc tgatggaagg gtgattccag
     4141 cttcagctga tgctttgtgt aactcttcca aaaagctggc acgactgatg atagaagcag
     4201 cagcaacagc taaatgatat tgttctcctt tagtaacgaa gtacagtttt tcttgtaatt
     4261 gatttttttc taaacgcgca tattttttaa aatttgcttc cggcgtaaac tgatcgatca
     4321 aaatcgcttc tggtttttct ggctcgattt tttttagcag caaatggatc gcttgattgt
     4381 gcaaagcgac tttcatccga acggcattgt attttggttg gatctcattg tatttttctg
     4441 gagataagac aagcagttta taggggatgg ttttttttaa gatgtcagca agctggatga
     4501 tttggctatc ttttaactct tttgaatctc taacaccaag tttacgtaat tctgcaagct
     4561 gatcacgttt cacataagca gcacatactg ttagcggacc aaaatagctg ccatttccga
     4621 cctcatcact acctaaaacg gataattgac tgatattttt tggcaaagaa gaggctgttt
     4681 ttgctggctt ggcttgttta tcctgtgcct ccccccattt atcagcttct attgctgcat
     4741 tcgtaccttg aaacatcact tttccggatg tataagcggt aatggtcgtc ccgtcttttt
     4801 ttgcagcaaa gctagtataa ggaatctgtt tatttaacaa ataagagcga tagtatgctt
     4861 tcatttgact aagttggtcg tttgttgctt tgataatgac gttgctcata aaattctcct
     4921 cgtttttata cataaaacta taccgcaact cacgactttt cgttttctaa gagattgcga
     4981 gacatagcat ttttttaaag tttatcatat aatagttaag tatgtgaaaa atatttgtgg
     5041 agaacggtga aagaaccaac ttcatggtac actaaataaa actttcatca aataggagga
     5101 aatgattcgt atggcgcacg aaaaaacacg ctacaaagca gtgattgcca accaaacata
     5161 tactattatc ggacgggaaa ccaagcatca tatggatatc gtaacgaagc tgatcaatga
     5221 acagttagca gaattgaaac aattatcacc acaaatggat aatgaacaag cagcaatctt
     5281 gatggctgtc aatgcgcttt cggatcaatt gaagaaacaa gaaagaatct tggagctgga
     5341 agaagaaaca gcagaattaa agaaaaaaat gatcaaattt acagagcttg aaaatcgtgt
     5401 gaaacgcatt gaagcaatcg agaacgaagc tcgggaagta ttgaaagaaa atggtcaagc
     5461 agatcatacc attaaaaatc atgtggaagc acaacagatt ttaaacgaga aacgaaaagg
     5521 acaaattaaa caaaaatctt ctaattaaga tccttttatc aaaataagaa aaatttaaaa
     5581 aagttagaag agaaaggaac gtagcatgct gagtttgctg attttatttt tattgctaat
     5641 tgcgtttttt tctggagcac gtcgcgggtt tgctttgcaa atcatattcg cgattggata
     5701 tgtattgtca tttattgctg cccagcattt ttacaaacca ttagcaagtc atttggaatt
     5761 atacattcca tacccagctg tcaccccaac atcaaagtta gcattttttg atcaggtatt
     5821 tgcttttcac ctagatgaag ctttttatgc aggaactgct tttctgatta ttttgctaat
     5881 tggctggctg cttacccgat ttgtgggggt ttttgtccat ggattgacgt atgtgccgat
     5941 cttgagacag gtagactgga ttgcaggagg catcctgtca ctaatcatgg cttacgttac
     6001 tatatttttg attttgcagt tacttgcctt tgttccatta gtgtggtcca aaatcaattc
     6061 ggcgagcata gtttcgctcg ttttatcgtt gagagaacgc catttcttac caacaagatg
     6121 tatgagcttt gggtaacgaa tattttgaat taaggaaaat agtgctggaa aatatttcgt
     6181 tttttgtaca gagtagttta tttaagaagg ctgcgtagcg tatgtccggc acagctcttt
     6241 tttcatagtg aaagataagg tgataaaatg aataaccgaa tccttgaaac attggaattt
     6301 gaaaaagtca aacagatggt cagacaattt gtagtgactg cacaaggaaa agaagaactg
     6361 gcagaattgg taccagtgag tgagaaacaa acgatcacaa actggcttca agaaactgag
     6421 gatggattga aagtgcaacg tctgcgaggc gggatcccta tacctaaact tgaaaatatc
     6481 cgtccccata tgaaacggat cgagatcgga gcagacttga atggagtgga gcttgcacaa
     6541 gtttccagag tattgtctac gacaagcgaa ttgaatcgtt tcattgatga tctttctgac
     6601 agtgaaatcg aatttgcacg tttgtatatg tgggcagatc agttagtcac gatccctgtt
     6661 ttgagccgcc gtttaaagga agcaatcgat gaagacggac gcgtgactga tgacgcttct
     6721 cctgagttaa agagcataag acagaacatt cggcgaagtg aacaagctgt tcgtgaacag
     6781 ttagatggga tcgtccgtgg gaaaaatgca aagtatttaa gtgatgcgat catcaccatg
     6841 cgaaatgacc gctacgttat cccagtcaaa caagaatacc gcggtgtatt tggcggtgta
     6901 gtccacgatc aaagtgcttc aggacagact ttatttattg aaccaaaaca agtggtggat
     6961 ttgaataatc gtttgcgtca ataccaaatc gctgaacgaa atgagattca acgtatttta
     7021 agtgaacttt ctgctgaact tgttccccat cgtcaagaaa tcatccacaa tgcttatgtg
     7081 attggtaaaa tggatctgat gaatgcaaaa gcacgatttg gtaaagaagt aaaagccatc
     7141 gtgccgggaa tcagtgaaga caatcatgtc gtcttgaaac aagcaagaca tccactgatc
     7201 gatcaggaaa aagtagtttc taatgacatc acaatcggaa aagactatca agccatcgtc
     7261 attacaggac ctaacacagg tgggaaaacg atcacgttga agactttagg tctgttacaa
     7321 ctgatggggc aagcaggatt accgattcct gcaggtgaag aaagtcaaat cggtattttt
     7381 gaggaagtat ttgcggatat aggagacgaa cagtcgattg agcaaagtct atcgaccttt
     7441 tcttctcata tgacgaacac tgtagatatt ttatcaaaag tcaatgaaaa aagtctggta
     7501 ttatttgacg agctcggtgc aggaacagat ccgcaagaag gtgctgctct agcaatcgca
     7561 atcttagatg atcttggtaa aaagtctgct tatgtgatgg caacaacgca ttatcctgaa
     7621 ttgaaagttt atggatacaa tcgggcaaat acgattaatg ccagcatgga gttcgatgtg
     7681 gatacactaa gtccaacgta tcgtttgctg attggcgttc ctggacgaag caatgcgttt
     7741 gaaatctcta gccgattagg tcttgataca gaagttattg atgaagcgaa gcagttgatg
     7801 aatgatgaga gccaagattt aaacgaaatg atcacagatt tagagaatcg ccggaaaatg
     7861 gcagagaccg aatatctgga aatgcgtcat tttgtttctg aggcacaaga acttcacgat
     7921 gatttgaaag aagcttacag ctatttcttt gaagaacgtg aaaaagaaat ggaaaaagca
     7981 aagaaaaaag caaacgaagt cgtgtcagag gcggaagaaa aagcagaaaa aatcattgct
     8041 gatattcgta aaatgcaaca gcagattggg caaggaaatg tgaaagagca tcagttgatc
     8101 gatgcaaaga cccaattagc aaatctacat caagaagaaa ctttgaagaa aaataaagta
     8161 ttgaagaagg cgaaagaaca aaaaacgttg aaaccgggag acgaagtgtt ggttacgact
     8221 tacggccaaa gagggacgct tttacgtaaa aacgggaatc agtggcaagt agaaatcggt
     8281 atattaaaaa tgaatgtgtc cgaagacgag ctgacgcctg ttgctcctca aaaagagccg
     8341 acacaacgag ttatccatgc tgtacgatct gaatctagta gtcacgtgcc gaatcaattg
     8401 gaccttcgag gaaaacgata cgaagaagcg ctaagtgagg tagatcaata tttggattct
     8461 gccattctag ctggttatcc tcaagtgact attgtccacg gaaaaggaac aggcgcactt
     8521 cgcaagggaa ttactgatta tcttaaaaac catcggagcg tcaaaagctt tgagtttgca
     8581 ccggcaaacc aaggcggaaa tggtgcgaca attgttaaat ttaagtaaag cttactaaag
     8641 gtaagcagat ggttcgctat ccaagtatag aaatgctata ctaatcatag attttaaaaa
     8701 tggaggtctt tgatatgtca caagtaatta cagataagac atttaatgaa gaaacagata
     8761 aaggacttgt attgattgat ttttgggcaa cttggtgtgg tccatgtcgt atgcaagcac
     8821 cgattttgga tcagttggaa caagaatatg atgaagaaga attcagaatt gctaaaatgg
     8881 atgtggatga aaatccagaa actcctcaac aattcggtat catgagcatt ccaacattaa
     8941 tgttgaaaaa agatggtcaa gtagtagaaa aagctgttgg tgttcattca aaagaacaac
     9001 tacgtcaaat gatcgatcaa tatttataaa aacttacagc aaaaataagc tgagggtggg
     9061 actgaatcca gttccgtcct cggttttttt gcggttgagt taaggttatt caaattaata
     9121 ttttttgagt ataattacgt tgtcaggctg gattgttttt ttgacagaca tgctataata
     9181 actttaattg aatatgaagg ggagtaccag atgaacgaac gaatcaaaaa caaattagct
     9241 ttacttcctg atcagcccgg ctgttacttg atgaaagata aaaatggaac gatcatttat
     9301 gtcggcaaag ccaaaatatt aaaaaatcgt gtccgctcat attttactgg cagccataat
     9361 acgaaaacgg aacggcttgt tagtgaaatc gaagactttg aatacatcgt tacagaatcg
     9421 aatatcgaag cattgctctt agagatcaat ctgataaaga aaaatgatcc taaatacaac
     9481 attatgttaa aggatgataa aacctatcct ttccttaaga taacgaatga aaaatatccc
     9541 cgtttggtga ttactcgtaa agtactaaaa gacaaagcac tgtattttgg accatatccc
     9601 gatgtcggtg cggcgaatga gacaaagaag atcctcgatc gattatttcc tctgagaaaa
     9661 tgcaaaccta gtcagacgaa ggagccttgt ctttactatc atttgggaca atgtctgtgt
     9721 ccttattatt tcgacgtaga tccgaaagtc tatacgaaaa tcgttgatga agtgaaaaag
     9781 tttttaaacg gaggacacga aaaaatcgag tcagagatcc atcagaaaat ggaaaaagct
     9841 gctgaaaaca tggagtttga aaaagctgca gaataccgag accagatccg tgccattgag
     9901 acaatcatga ctcgccaaaa aatgacaaat actgatttac tggatcgaga tgtatttggt
     9961 tatgcgattg acaaaggatg gatgtgtgtc caagtgtttt tcgtaagaca aggaaagcta
    10021 attgaacgag atgtttcaat gtttcctttc tataacgaag cagaagaaga tttcttgact
    10081 tacctcggtc agttttatca ggaaaatgag catttcattc ctaaagaagt cttgattcct
    10141 gatacgatcg acaagcccag tgtagaagct ttgttagcta cgaaagtgct gcaaccaaaa
    10201 cgaggagaaa agaaaaaatt agttgaattg gcgaataaga atgcctcagt tgctttgaat
    10261 gaacggtttg atctgattgc cagaaaacag gaacggacga tcggagcagt tgaacgttta
    10321 ggaaacgcga tgaatatccc gaccccaata cgtatcgagt cttttgataa ctctaatatc
    10381 atggggacga atcctgtggc tgcgatggtc gtattcattg atggaaaacc ggcaagaaaa
    10441 gaatatcgga aatataaaat aaaaacagtc aacggaccgg atgattatgc atcaatgaga
    10501 gaagtcatct atcgcagata ttcacgagtg attcgcgaag ggttgccttt acctgatttg
    10561 atcttgatcg atggaggaaa aggacaagta gatgtggcaa aagatgtctt agaaaatcaa
    10621 ttaggaatcg atattcctat tgctggcatg gcaaaaaacg ataaacataa aacaaatgag
    10681 ttgctattcg gtatagattt ggctgtagta ccgcttgagc gaaattcacc agagtttttc
    10741 ttactccagc gaatccaaga tgaagtccat cgattcgcaa tcactttcca tcggcagcta
    10801 cgcagtaaaa acagttttgc ttcccgatta gatgaaattg aaaggttagg accaaaacga
    10861 aaaaaaatgc tgcttaaaga gttcaaatcg ttgaaaaata tcacagcagc cacgacagaa
    10921 gaattacaag aaatcggact gccgaaaaat gtcgcacaaa acgtatttga taaattacat
    10981 caggaatgag gaaagggcac tttacaaaga aagtaaagtg tcctatcatt ttatgtacaa
    11041 caaaaagagc gtttacgttt tgttggtaaa taataaatcg cttacgtctg gtgtctaagg
    11101 atatatgtga tttcttgaag aaggagtgaa gaaatgttag aagtcaagga tcttgttaaa
    11161 acttttggga actacacagc tgtcgatcat gtctctttcc aaatccccga tggaaaaatc
    11221 atggggctaa ttggccaaaa cggggctggg aagacaacga cttttcgttt gatcttggat
    11281 tttttgacac aagaccaagg agaagtgtta tggaatggcc atcatcttgg ggagaaagat
    11341 tatgatgtca tcggttatct tccggaggaa cggggacttt atccgaaagt aacgattcaa
    11401 gaccaactgc tttattttgc agaacttaga gggaaatcaa gaaaagagat tgaaccaaaa
    11461 atcgattttt ggatggaaaa atttcaggta aaagggaaaa agacggataa agtaaaatct
    11521 ttgtctaaag gaaatcagca aaaagtccag ctgatcgcca cgttaatcca tgaacctaaa
    11581 ttgatcattt tggatgagcc attcagcggg ttagatccgg taaatgctga attgctaaaa
    11641 gatggaatat tggaattgaa aaaacatggt tcatgtgtca ttttttctag ccacaatatg
    11701 gataatgtag aaaaaatctg cgatcatttg atcatgttgc gaaacggaaa aatggttttg
    11761 aatggaaaag tacatgagat tcgtgaatca tttggccgta cgaagttatt cttggaatct
    11821 ggtttaagcc aacaggaagt agaagaaatc gaaggcgtga aaaaagtgat ccttcatgaa
    11881 gacaatacgt tagagattac cttagaagat cctgaagctg ggaaagaaat ctttactcgt
    11941 gcgactcagt tcggctacat tcctatgttt aaccagcaac cgccaacttt ggaagagatt
    12001 ttcaaattga aagcaggtga aatcgatgcg taagttttgg attattacca aagatgtcta
    12061 tttaaaaaat gttaagtcga tttctttctt gatcatgata ttagtccctt ttatcatgct
    12121 gggtatcatt tatgtagcag gaaactttgc tcaacaaaat agtgatgtca ataaaatcgg
    12181 cgtattggct gataatgagc aaatcagtca gcaaatgagc caagtcaaaa cagatgattt
    12241 tgagttcaaa gcagttgatt ctgaagaaaa agcacaaaaa gagctttcgg aagaaaaaat
    12301 cgatgcgtat ttagtagtct caactgagaa tgataagata aatgggatgc tttatagcga
    12361 aaattcatta ggacaaagta cacaactact gatacaacaa caactgactg gcttgcaatc
    12421 gatgcttcga gcaagtcaat taggcatttc accagaagaa gtagctagct tgagccaaca
    12481 agcaagtttg actcgacaaa aagtcaattt tgattcaaaa ggaaaaatga cagttggaga
    12541 agataactcg acagtccaat atgtcgtaag ttatgtagca acgattgtct tgttcattat
    12601 tattttgacg tatgcgcaga tcattgcaca agagatcgct tctgaaaaag gcacgcggat
    12661 catggaagtg atcctgtcca gtacgactgc acaaaagcat ttttacggaa aactaacagg
    12721 tgttttactt gttgcattaa cgcaaatggc attatacggt attatttttg gtataggata
    12781 cagtcaattc aaagatatgg aaatggtcaa aaatatatta gacggtatct cattggataa
    12841 cttgattgga ccattcttgt ggtattctct tctattcatg atctttggca ttttgatttt
    12901 ttctgtttta gcagctttat gtggctcttt ggtcaacaaa gcagaagata cagcaaaagc
    12961 aattttgcca gtgacttatc tatctctagg cggatatatg cttggattga tactaggagc
    13021 ttcagatcca aacaatatcg ttattcggat cacttcgtat attccattct tatcttcata
    13081 tattatgccg gttcgcttag caaatgagac agtcggtatg ggtggagcat tgatttcctt
    13141 gttggtactt gtagccgtaa cattcttatt gatgatcttg tctgcaaaca tgtacaaatc
    13201 gaatgtactc gtgtatagcg aaggcggatt atggagttca ttgaaacaat cgatttcaat
    13261 tatgagaaat gaacgaaaaa aagcataaaa tatagaaaaa tatagaagga agctggacat
    13321 ttattgttcc cagcttcttt tttgtataga cctatttcta tttttttatt atattggata
    13381 tatatattca ttgaaaatag agcataacaa ctttataatg ttgagtatac caatttttgg
    13441 aggcgtttta atggaccgct atttagattt actttcagag aagttcccta caacggaaga
    13501 tgtcatcacg gaaatcatca atctcgaagc aattttgcag cttccaaaag gaacagagtt
    13561 atttttgagc gatatacatg gcgaatttcc tgcttttgat catattctgc gtatcggttc
    13621 aggaaatctt aaagaaaaga tcaaagacct tttctctgaa caaatgacgg aaacggagat
    13681 cagccaattc actattttta cggcttatcc agaatatgtg ttgacgactg attggtacaa
    13741 aaaacaagat aaatcccagc tgatccatcg tttgatcgat cttttacggt ttacgacagt
    13801 caaatacact cgatcaaaag ttcgaaaagg attgccgaaa gaatatagct atatcattga
    13861 ggaactcttg tatatcgatg atcgtatcaa cgggaaaaaa gactatgtaa aaaaaatcat
    13921 tgaacaactt gtcttgatga aggaagaaga aaaattttta acaaaacttg cgcagacgat
    13981 ccaaaaatct gtcatcgatc atttgcatat cgtgggtgat atttttgatc gaggaactca
    14041 agcagccaaa gtaatggatc gaatcatgga tttttcctct gtagatatcc agtgggggaa
    14101 tcatgatatt ttgtggattg gtgcgtattg cggctctgag gcatgtttat tgacgctttt
    14161 acggattgcc gcacgctaca actatttatt tgaattagag aaagaatatg gattaaattt
    14221 acgtccatta ttttcttttg cccatcaaac ctatcagaag aatccagtat ttacccctaa
    14281 aaacaggaaa gatctatctg aaagagagca agaagaatta gaaaagatcc atcaagcact
    14341 ttctatcctc caattcaaat cagaacatca gttgttgaat cgacgacctg aatttaaaat
    14401 ggacgatcgt aaattgttgg aaaaaatcaa ctatgaggaa tcaacgatcg ttttagaagg
    14461 tcagctctat gagttgaaag ggacttgttt tcaaacgatc cagccagatg atcctaaaaa
    14521 attcttggaa gaggaacaaa aagtgatcga tagtttgatg tattcattcc agcactcgtt
    14581 aagaatgcaa aaacacatga cgtttttgat cgaaaaaggg ggaatgtatc tcacgaacaa
    14641 tcagcatgta ctgttccatg gctgtatacc tgtagatgaa aaggggcagc tcttagcatt
    14701 tgaattagac caatcttatc gcggaaaaga actgctggga ttttttgaaa aacagataac
    14761 tgaaagcgca aaagatttga ctgcaaatga agatttggcg accgatttga tttggtatgc
    14821 gtggagcggg ccattttcgc ctttattcgg caagagtaaa atggcaactt ttgagcgcta
    14881 ttttctcaca gagcctcata cgcacgtcga aaaaagcaat ccctattatc acttgcgaga
    14941 tgaagagtgg ttcagtgaaa aaattttggc agaattcgat gcaaaagaag aaggttcagc
    15001 aatcatcaat ggacacacgc cagtaaaagt aaaaaaagga gagtcaccga tcaaagcaaa
    15061 cggaaaagca tttgttattg acggaggtct atccaaagct tatcaaaaaa caaccgggat
    15121 tgctggctac tcattactat gcaattctta cggtttccaa atcgtgacac attatccgtt
    15181 cttgcctatt gaacaacaat ttgcaaaaaa atctgatcaa acgaatgtaa aaaaagtgat
    15241 cgagcagcag ctcccacgca aacttaatcg gaatacgaca aaaggaatgg aattacagca
    15301 acagattact caactccaaa ggctgttgga ttatcgaaag gacgattgag aaaaatgcta
    15361 gccaacattg ctcaatagac taaaaagcga ctgcaacaaa aggcgtgtcg cagtctcctt
    15421 cggaattagc tcaaaaaacg gtaaaatatt agaagctatt tttctgttta tttctctaat
    15481 actcaggtca gggcgctgat ttcacgacct cttccttaaa cggtgttctc ctgccaactc
    15541 ctcggtcaca agtcacaata tccaacaaac atgagaagct gttttttgaa attgctcctt
    15601 gtgactcaga gttgaacggc aggctcacga cctaatcaga aaaacgatta aaattcattc
    15661 acaaagaaat cgaataaacg ctgttcagat gaatctacac gatgagaaag ttccggatgc
    15721 cattggacac caaggatttt gtgcatatta tctttacttt ggactgcttc aatgagtcca
    15781 tcgttggaaa aggcgatggc ttttaacaaa ggagaaagat ctttgactgc ctgatgatga
    15841 taggaattca caaagtatga atcagggaga aggtcactta ataaactgtc cgaaacgatt
    15901 ttgacttcgt gagttgcaaa ctgcggggca gtgggctgtt gttcgtgttt tacggaccat
    15961 tttggatata atgaaagatc ttgataaagt gttccctcca acacgacatt caatagttga
    16021 agtcctcggc agactgcaaa aatcggtttt ctttgtttca atgcttccaa gatcaatgcc
    16081 tgttcgaatt ggtctcgttg aatatttgtt tccgtcagtt ttggatgagg gtcttccatg
    16141 taaagttgcg gactaacatc ctgaccgcct gcaagaatga gtttgtcgat tttctcaatt
    16201 agttgaggcg ctgaatctgg agacatgact ggcagaataa gaggtaatcc ttgagcatct
    16261 aaaactgcat ccacaaatcc ctgcggtgta tagcttactt gatttccttg gaaagtatct
    16321 gtcgctcgaa tcagttgatt cccggcaatg ccgatgattg gtttcatttt tattccccct
    16381 aaagtagact aattgtaaca aggatttaaa aaaatttaaa ctatgtaaga cttgccccta
    16441 tacttatctg ttcttgctgt gttactatct gtaaagagaa ttttcgtgtg aaattcatga
    16501 cgatagttga aaatattgct taatttgtta taataaaaag taagcagaaa taaaaaggtt
    16561 gggcgtaagc ttcaaggatc tagttcttca aggggtgaga aggacatttt attgaagggg
    16621 cctgcctata ctaagttatc tttagaggta catactttca atgaaaaaag aaagcatgta
    16681 cctctttttt aaatcgaaag gaagtgaagc aagtgttaga agctttggaa caaatccgaa
    16741 aagcagaaga agaaaatgag aagcgtgctc gaaccctacg gcagcagttg aagacctatg
    16801 cagaagaaaa agagcaagaa ttggagataa tccaaacgaa cagtcggcaa cagctgaaac
    16861 agttggtaga agaaaaagaa caattcgaaa agcaagaatt agaaaaagaa aaaatgaaat
    16921 tgaaagcaga agcagacaag gtgcaagcaa ctttgaaaga ccaatatcaa aagcatcaga
    16981 gtcaagcaat cggtgcaatc attgaaaggg tgaaagaaac ttatggccgt cactaagatg
    17041 gaaaaagtga ccctcatttc agataaaaga aatcaggaga ggattttaca agcagtacaa
    17101 ggaatccaaa atgttgaaat ccgggatctg tatcaaaata gaacaaacaa ccagtgggta
    17161 gaaaaatatt ttccagatac cacgattatt gataaagaag caaaattaag cgctttaggc
    17221 aatcggctga aagacatcca agaagctatc caattcatac aacaccacgg cgatagccgt
    17281 caaaagaaac tccatttgaa acgtcaagaa ctttccttac aagacttgga agcaaattat
    17341 tccgaagaaa atttcatgaa taaattagaa gaagtccttg atttaaaaga aaagtgggaa
    17401 cagctaactg aaaaacgtga tcgtttagaa gaagaggaag aatggcttct tcattggcaa
    17461 cagctggata ttgctccaag tatgtatgca agtaagacga ccatttttga gatggggact
    17521 gtgagtgcag caaattttga aagtttcaag gaagaactaa gtaagcttga tgacgtttat
    17581 atggaagaaa tagattatac gccaaaacaa gtttatcttt cgtatgttgc tttaaaaaaa
    17641 tcagttgctg ctatagaaga aattgctggg aaatacgggt tcaataaaga aacgtatcct
    17701 tatgaacaaa atcctaaaga aaaattgcaa gaaaccaaac agcaattatc cattgttcac
    17761 gagcagcaaa aaaagataac gacagcgtta ggtcagtgca gcggctatat ccaagaattt
    17821 gaatgggtag aagaagtcac acttgccgtt gcagaacggg aaaaagtaaa agaacggttt
    17881 atccatgcca cttatctgat cgttttgcaa ggttgggtag atgcagaaga aaaacagaat
    17941 ttgatgcatg tgttaaacga aactgtatca gaagaagata tatacgttag tttcgaaaca
    18001 ccaaactcag aagagattca gcaggaagtc cctactaaat tgaaaaacca tccgcttatc
    18061 gaaccttttg aattgctgac tgaaatgtac agtctgccaa aatatgaaga agtagatcca
    18121 acgccttgga tgacaccatt ttatcttgtc ttcttcggga tgatggtcgc agatgttggt
    18181 tacggattgc tgatgctcat tgggacaata ctagctcaaa aactatttgt tttaccccgc
    18241 gggatgaaac gtttcgtaaa attctttgag attctctcgg taccatcgat catctgggga
    18301 ttgatctaca gttcattttt cggacaggca ttaccaaaag aactatttgg tatccgcttg
    18361 cctttcccaa tcctatctac gacggatgac gtcaatacga ttttgatttt atccgtgatt
    18421 ttcggtctga tacagatctt ggttggatta ttcgtggcag caaaggaaaa tatcaggagg
    18481 aaagattact tgagtgctgt cagtgatggt tttgcctggc aagggatcct gattgggatc
    18541 gttattgcat tgattggtgt catgttgata aaaaatacag catttgttta tcttggcgcg
    18601 gggctatcga tcattgctgc tttatgtatc ttaatcgtgc cgattatcca atcgccgtct
    18661 aaagtcaagg gtgctgccaa aggagcgtat aatctttacg gattgactgg ttatatcggt
    18721 gatttagtca gttatacacg tttgatggca ctaggtattt caggcggaag tattggcgcg
    18781 gcattcaata tgctagttgc tttcatgccg ccagccgctc gattcagtgt agggatcctt
    18841 ttgatcatcg ctttacacgc actgaatatg tttttgacac tgttaagtgc ctatgtccat
    18901 ggtgcacgat tgcagtatgt cgaatttttt ggaaaatttt atacaggagg cggacgtgcc
    18961 tttgacccgc tgaaaacggc ggaaaaatac gtcaacatca atcataagaa aaaaaacaat
    19021 aaataattgg aggattttta tacaatgatg gattacttaa ttagtcaaaa tggtggaatg
    19081 gtatttgcag tattagcgat ggcaacagcg acgatttttt cagggattgg ttcagctaaa
    19141 ggtgttggaa tgacaggtga ggcagcagca gcgttaacaa caagtcaacc cgaaaaattc
    19201 ggacaggctt tgattctgca attgcttcca ggtacgcaag ggttgtatgg attcgttatc
    19261 gcctttttga tttttatcaa cttaggaaat gatatgtcag ttgtacaagg attgaacttt
    19321 ctaggcgctt ctctgccgat tgcttttaca ggtttgtttt caggtatcgc ccaagggaaa
    19381 gttgcagcag caggtatcca aattttagcg aaaaaacctg aacatgctac aaaaggaatc
    19441 atttttgctg cgatggttga aacatatgcg attttaggtt tcgttatttc cttcctatta
    19501 gtattgaatg cctaagcata aaggaggaaa agcagatgaa cgccattgaa aaaatcatct
    19561 ctcagatgaa tgaagcagca gagcaagaaa gagcggcgtt agagcaagaa gagcgaatga
    19621 agatcgatca aaactttgaa caaaaacgta cgcaaatcga aacagaacat caaaaacaaa
    19681 aagaaaaaca aatcgaatta cttgaaaaaa aataccgtca gcttaggaat cgccaacaag
    19741 tagaagtacg acaagaaaat ttgaatgcga agcaggaatt tttacgacga ttgtttgcag
    19801 atgcaatagc tgaaatggag aattgggatg aatctgaaca aatccagttt atcaaaaatt
    19861 cattgtattc attacctttg acaggcaaag taacattcat tgctggagaa aaatcagcag
    19921 cttatctttc tcagacattg ctggatgaat ggaacaatga acttcccttt atgatggttt
    19981 taagtgatga aactgttgct gatcaagcgg gttttctgat aaacgatcaa ggagttcagt
    20041 ataattttct tttcagcagc cttgtacaag atatccaagg aacgatgagt tttgagattg
    20101 ccaatcaatt attcgaataa ggagggatca gtgtatggaa taccacgaac taaatccctt
    20161 gattcgcgga agagagcttg aacttttgtc caatgagaca tttgaacgga tgattcaaac
    20221 aaattctgtc gaagcacttg gtgaaatatt gaaatccacg atttacagtc catatattta
    20281 tgacggatat gaatatgact tcgaacagaa tcttttaaag gagcagagcc gtctatttcg
    20341 ctggttaaag gaatcagcac cggagcctga aatcgtttgg atctatacga tgcgctatac
    20401 tttccataat ctgaaggttt tgacaaaagc cgagatgaca aatcaaaatc tggaccattt
    20461 gtatatcgac gatggatttt attcattaga aaccttgaag gatgccattc atacgcaagt
    20521 ttcaaatgaa ctccctgaaa gcattatgga cagtatacga gaagtacgtg attattttga
    20581 agagtctacc attcttcagg gaattgacgt gatttatgat cgacagtttt tgacagagca
    20641 gcgacggctt gccgataagc taggatatca agaattgctg acagaagtca ttgcttttat
    20701 tgacttgacc aatatcacaa cgatggctcg aggaatattg cagaaaagaa catatgggtt
    20761 catgacgacg gtactttcca gctcgggaag tatcgaaaaa gaagtatttt tgaactttgt
    20821 agataaagat atggaagatt acacgaattt tctattagag acagattatg ctgattttct
    20881 gcgccctgtc atccgtgagg gaaatcttga tctcgtaaag cttgaacagt tgaaagatga
    20941 ttatctaagt tcattgtatc aagaagcaca aacccaggct ttcggaccac tgccactact
    21001 agcctttttg aacgcaaaag aggtagaaag caaaaacctt cgtttactgg ttatcggcaa
    21061 aagaagccat ttcgaaccag aaacaatcag aaagcgggtg agacaagtct atgacgcata
    21121 agatcggtgt agttggcgac aaagattctg ttttgccatt caaattgttt ggctttgatg
    21181 ttcgttatgg tacaaccaag caaaccgttc gtcaggcaat tgaagaaatg gcaaagaatg
    21241 agtatggcgt gatttatgtc acagagcaat gtgcagcatt gagcccagaa acgatcgaac
    21301 gttacaagga acaaatgata cctgctatcg tattgattcc caatcatcaa ggatcattag
    21361 ggatagggct ggaagaaatt caaaaaaacg tagaaaaagc agtaggtcaa aatatcttat
    21421 agtatgaaaa ggagatagaa attttgcaaa ttggaaaaat cataaaagtc tccggacctc
    21481 ttgtaatggc tgagaacatg tctgatgcca gtatccaaga catgtgtcta gtgggagatc
    21541 taggagttat cggagaaatt atcgagatgc gtggcgatgt ggcgtctatt caagtatatg
    21601 aagagacttc tggaattggt ccaggcgaac cggttcgctc tactggtgaa gctttatctg
    21661 ttgaattagg accagggatc atttcacaaa tgtttgacgg aatccaacgg ccattagata
    21721 cctttatgga aatcacacaa agtaatttct tagggcgcgg agttcaattg cccgcattgg
    21781 accatgagaa aaaatggtgg ttcgaaccaa cagtagaagc cggagaaacg gtgagtgctg
    21841 gagatgttat cgggatcgta gaagaaacaa aagttataaa acataaaatc atggtgccaa
    21901 atgggataaa aggaacgatc aaaacaatcg aaaaaggttc attcactatt gatgaaacag
    21961 tatgtgtcat tgaaaccgaa aatggtgaca aaaatttgac gatgcttcaa aaatggccag
    22021 taagacgtag tcggccaatc aaagaaaaat taaacccgga tgcacccatg atcactggac
    22081 agcgagtgat tgatacattc ttcccagtga caaaaggagg agcagcagct gtacctggtc
    22141 cttttggtgc agggaaaaca gtcgttcagc atcaaattgc aaaatgggcc gatgtggaca
    22201 tggttgtcta tgtgggatgt ggcgagcgtg gaaatgagat gacggatgtt ttaaatgaat
    22261 tccctgaatt gattgaccct aatacaggag aatcgttgat ggaacgtacg gtattgattg
    22321 ctaatacatc aaacatgcca gtagcagctc gagaagcttc tatctatacg ggaatcacaa
    22381 ttgcggaata tttccgtgac atgggttatg acgttgctat catggcagac tccacttcac
    22441 gatgggcaga agcacttcgt gagatgagcg gacgtttgga agaaatgcct ggtgatgaag
    22501 gttatccagc ttatcttggt tcacggcttg cggaatacta tgaacgatct ggaagagtca
    22561 ttgcattagg atcagaaggt cgcgaaggaa gtataaccgc aatcagtgct gtatccccgt
    22621 ctggagggga tatttcggaa ccagtcacac aaaatacatt gcgggtcgta aaagtattct
    22681 ggggtcttga ttccagttta gcccaaaaaa gacattttcc ttcgatcgat tggatacaaa
    22741 gctattctct ttattcaaca gaagtaggga agtatatgga tcatatcctt caagctgact
    22801 gggcatccat ggtcaccgag ggcatgcgta ttttgcagga agaacagcag ctgaatgaaa
    22861 tcgtgcgttt ggtagggatt gattcccttt cagataatga ccgtctgaca ttagaagtgg
    22921 caaaatccat tcgtgaagat tatttgcagc aaaatgcttt tgatgatgtc gatacattca
    22981 cttcaagaga gaaacaattc aaaatgttga agatgatctt gacctttggt gaagaaggac
    23041 gccgtgcgtt gtcgttaggt gcttatttaa gtgagatcat ggacggtacc gtatcgattc
    23101 gtgaaaggat cggacgaagc aaatatatcc cggaaacaga attagaaaaa ctagatcaaa
    23161 ttattggtga aatcaagcaa acgatgcaaa cgatcgtttc agaaggaggc atgagcaatg
    23221 attaaagaat atcgtacgat cggtgaagtc gtcggtccat tgatggcggt tgaaaaagtt
    23281 gctggtgtca aatatgaaga attaatcgaa gtccgtatgc aaaatggcga gattcgtcgt
    23341 ggacaagtat tggaagtcca agaagataaa gcaatggtcc aaatattcga aggaacaagt
    23401 ggtatcaatt tacgtgactc ttctgtacga tttttaggcc atcctttaga actaggtgta
    23461 tcggaagaca tgatcggtcg agtattcgat ggtttgggac gaccgaaaga taacggacca
    23521 gagatcctgc ctgaaaagca tttggatatt aacggagaag taatcaatcc agtagctcgg
    23581 gattatccag atgagtttat ccaaacaggt atctctgcaa tcgatcattt gaatacatta
    23641 gttcgtgggc aaaaattacc agtattttct ggctctggtc ttcctcataa agaattagct
    23701 gctcagatcg cgcgacaagc aacggtactc gattcatcag atgatttcgc cgtagtcttt
    23761 gctgctattg gaatcacttt tgaagaagct gaatttttca tggaagattt tcgtcaaacg
    23821 ggcgcaatcg accgttctgt catgtttatg aatttagcaa acgaccctgc aatcgaacgt
    23881 atcgctactc ctcgtatggc tttgactgct gcagaatatc ttgcttatga aaagggaatg
    23941 catgttttgg ttatcatgac agacatgacc aactatgcag aggcattgcg agagatttct
    24001 gcagcacggc gagaagttcc aggaagaaga ggatatcctg ggtatcttta cacgaactta
    24061 gctactttat tcgaacgagc aggaagaatc cgcggactaa aaggatcggt tactcaaatt
    24121 ccgattctaa caatgccaga agacgataaa actcatccaa tccctgactt gactggttat
    24181 attacagaag gacagatcat cttgactaga gagctttaca agagtgggat tcagccacca
    24241 attgatgtac tcccgtctct ttcgcggtta aaagataagg gaactggacc cggaaagaca
    24301 agagaagacc atgcagctac gatgaatcag ctttttgcag cctatgcaca aggaaaacag
    24361 gcaaaagaat tagccgtagt tttaggtgaa tctgctttat ctgatgtaga caagatctat
    24421 gcgaagtttg ctgagcggtt tgaaaaagaa tatgtcaatc aaggattcta cacgaaccgt
    24481 tcgattactg aaacacttga tctagggtgg gaactgctat cgatgttgcc aagaaccgaa
    24541 ttaaaacgaa tcaaagacga catgctggac aaatatctgc cagaagggaa gtgagttctg
    24601 atggcacgat tgaatgtgaa tccgacccgc atggaattga ctcgattgaa aaaacagttg
    24661 acaactgcca caagagggca taaattatta aaagataagc aagatgaact gatgcgtcaa
    24721 ttcatcctgt tgattcgaaa gaacaaccag ctgcgtcaag aaatggaaga gtctatgaca
    24781 gaagcaatgt ctgattttgt attggcaaat gcttcgatca cggaaccttt catcgaagaa
    24841 ttatttgttt tgccggcgga aaacgttgat ttgtctatcg tagacaaaaa tatcatgagt
    24901 gtaaaagtac ctgtgatgaa ttttgaatat gatgaacagc tgacagacac acctttggaa
    24961 tacgggtatt tgaattccag tgccgaactg gatcggtcaa tcgatcggtt tactaccctg
    25021 cttccaaaac ttttggaact gacagaaatc gaaaaaactt gtcaactgat ggcgagtgaa
    25081 atcgaaaaga cacgtcggcg agtcaatgca ttagaatata tgacgatccc tcaattagaa
    25141 gaaacgatct attatattcg tatgaagctg gaagaaaacg agcgcgcaga agtaacaagg
    25201 ttgatcaaag taaaaaatat gggtaccact gactaagtgg tatctttttt taaaagagga
    25261 aaacgatgat taaacaaagc aaacaaataa agaaacgttt gccggctgtt cagcttctcg
    25321 cattaggatt ttttctcctg attttagttg gcggcggcct acttacccta ccatttttta
    25381 gtaatagtgg gcatgggaca aattttatcg atgcgctttt cactgcaact tctgctgtct
    25441 gtgtaacggg attgacaaca ttgaatacag cagagcattg gaacagtgct ggtcaatttt
    25501 tgatcatgct tttgattgag atcggcggtc ttggttttat gatggttccg attctatgct
    25561 ttgcattgat aaagaagaaa gtcagtctca gcacacgtat tatattaaaa gaagcgctga
    25621 atctagaaga agtatcaggt gtgatgaagc tcatgcttta catcctaaaa ttggcctttg
    25681 ttatccaatt gattggtacg gttgctttat cttttgtatt tgtgcctgaa tttggctggt
    25741 tgaaaggtat ctggtacagt ttgttccatg caatctctag tttttgtaat gcagggtttg
    25801 atttgttagg agacagttta gccaatcacc agcaaaatgt ttatttgatc atggtggtat
    25861 ctgctttgat catttctggt gggttaggct tcatcgtatg gcgtgattta ctggaatatc
    25921 atcagaaaaa gaagatgacg attcattcaa aaatcgcatt aaccgttaca ggtacccttt
    25981 tattaggcgg attcgttgtg ttctttttca cagagcgcaa tggggcaacg ctagtggaag
    26041 ggaatatgtt tcaacgattg gcaaacacct tttttatgag tgtgacacct agaacggctg
    26101 gatattattc agttgattac ctccatatga gccatgcagg tctgatttta acgatgtttt
    26161 tgatgtacat cggcggaaca tctgggtcga cggccggcgg attgaaaaca actacgttag
    26221 gtgtgttgct gatccagatg cgttcgatgt tgaaaggacg tacgagagcg gaagcatttg
    26281 gccgtaccat ccgacaaggc gcagtgttgc gggcacttac tttatttttt gttacgttga
    26341 cgttgtgtgt agtcgccatc atgattttat cggtgactga aacgatacct aaaacttctg
    26401 gcatcgaata tattgctttt gaggtcttct ctgcgtttgg tacagtaggt cttacgatgg
    26461 gattgacgcc agacttgact gcaataggga aattgatcat tatagcgctt atgtacattg
    26521 gacgagttgg tgtattgact gtggtcttct ctctgatgac aaaagcacaa agaacacaat
    26581 caaaatacaa atatccagaa gaaagtgtca tgattggata atttcatctt ctgacagaaa
    26641 cctagtgatt tagtaaaata agaaaataaa ataatttttc ttgcttcttg gatactttaa
    26701 gtgcaaaatg ttcgtttatt ttttgaggca ctacccgttg ttgaacctcg ggtagtgcct
    26761 cttttttatt gaaaggaatc aataagattt gattccttta tttatcaata ggttataatt
    26821 catatttttt tattttttct tttacgatac ttagtccaga agctaaaatt tcaaggttat
    26881 tagaggatag ggaaattcga acgaatgcat ctggttgcgc acgtgtagta aagcgtctgg
    26941 aatgataaag tcggataccg ttagcaagaa agtcattctc aattatctta gggtcaatct
    27001 caggatcaac tggaagaatt cgatagtaag ggacacgatt cgttggtttt tctactttga
    27061 aaagagaatc aaaaagctga ttggctcttt ttgtaagact taatttagtt tctaagatcc
    27121 tatttgcttc atttgaatgg aggatttgag tgatgacttc cacatctaat ccagaagttt
    27181 tcacatttat attaaaaatt gcttgttcta tcttgctttg gacatgtact ggaactacaa
    27241 gataagcaac tcgcaatcca gaacaaataa attttgtcat tccagctaag tagattgttt
    27301 ggttaggtaa taaattttgg aaaggaggaa tgatcgtatg ttgatagtaa gttgttaaaa
    27361 atgaatggat atcatcttca ataacccata gaccttcttt ttcaatcact tcggctaatt
    27421 gctctcgtct tttgtgagag atttgaaacc ctataggatt attacaagaa ggcataagaa
    27481 agacaccatg aatcttcttt tttctacatt cctgaatcaa tatatcaggt cgcattcctt
    27541 gctcgtcaaa atcaattgga acgatctcta aatgatataa ctgagccaat tcaatgaaat
    27601 tagcgtatgt atatcggtct gttgctattc tatcacctgg agagaaaagt gcagaaagtg
    27661 tgatagccaa tccattttga acaccggaaa caatagatag agattctaag gacggaatga
    27721 ctccttgagt ttttaaccaa tttgctgcag tagtcaattg aaaatttgtg cctttgggat
    27781 cttggtaatt aagcaaacca acaagatttg ggttattact agttgattga ataaacggga
    27841 caacgaattt attacattct tcaaaagaac taaccagacc aaaatcaatg atatgttcgg
    27901 ttacttcatt ggtagagatg gtgatggact caaagacatt gggagaaata aatgtgccac
    27961 ttccaatatt cgtatacagc aaacctttct caattccata tttataagcc ttgccaacgg
    28021 tagtaaaatt taaatctaaa taatcagcta gctctctttg ggagggtaac tgagtgtttt
    28081 tctgtaattt attctttaaa atatcttgtt ctaattgttg aatcaacgat cgataataag
    28141 gacgcttcaa tttttgtttg tctggtcgcc aagttaacgg gtaattgtca taagaattaa
    28201 taggcatttt ctccactcct taaaattgta taccatacaa ttttaccatt gattgtattg
    28261 attttagaag ttaatctttt gtgaggaggg aattgtctat gaaaattatt aattttgagg
    28321 aagtagtagc ttcttttagt tttgaagaag cgattcaagt aatgaaaaaa tgtttttttg
    28381 attatgaaag tggtaaaatc agccaaaatc ctcgattggt ggaaatttta cctgacggaa
    28441 aaggcaaaaa tatgtttgcg atgatgcctg cttatttggg ggagaacaga tattttggca
    28501 caaaagtcat taccgctttt ccggataatc atcagtatca tttaccttct catttaggag
    28561 agattttctt gtttgatgct caaaacggac tacccaaagc attgataaac gcaaatgcag
    28621 tcacctggat tcgaacagct gctgtatctg cgctggcaac agattactta gcaaatccgc
    28681 atgcagaaac acttgcttta attggagctg gtcaacaagc attgtcgcat ttgactgcga
    28741 tttctaccat tcgtcccatc actacagtgt ttgtttacga tattttagaa gaaagacgaa
    28801 atacttttat cgctaaagct aaaaaacagt atcctaaaat aacgtttata aatgcaatat
    28861 cagtagaaga tgcagtacgt ccagcggaaa tcatttgtac gttgacctct agtaaagagg
    28921 catttcttca aaaagagtgg gtaagggaag acgcacatat caatgctgtg ggcacattta
    28981 ctcctgatac aagagagata agtacatcgt tgatggaaac ttctcaaatc tatgtagatg
    29041 attactcagc tgccttaaca gaaagcgggg acctgcttat agccatggca gaaggagatt
    29101 tagaggaatc ttgtattgtt ggtacattag gcgaattagt ttctgagaaa aaaacatttt
    29161 ataaaggaga atcgaggccg acgattttcg acgcagttgg tctagctatt gaagatcttt
    29221 gttgtgcaga atatatttat aacaaattag agaaaggggc tttaaaatga acttagatta
    29281 ttacgtagtt gatgcatttg cagatgaggt gttcaaagga aatccagcag cggtttatgt
    29341 gctagaagaa tggctgccag aaggtacgat gcaaaaaatt gccattgaaa acaatttatc
    29401 tgaaacagca tttacagtga agaaaaacca agaatttgag cttagatggt ttacgccaga
    29461 tcgagaaatc gatctttgtg gtcacgcgac attagcaaca gcatttgttt tatttaacta
    29521 ttacaaaata cctgatgaaa cgattaaatt ttctactcaa agcggtaatc tatttgtgac
    29581 aagaattaaa gattattatt acatggattt tcctagtatt atgccgaaaa aagtgccgat
    29641 attgtctgaa tatgaagaag cgattggggc taagattaaa gaagcctact tagcacgtga
    29701 tttatttttc gtattagaag atgaaaaaac ggtggctaaa ttgcagccag attttacagc
    29761 tatcaaaaat tttgaattag gaataggagt catcgtgaca gcagaaagtg tgcaaaaaga
    29821 ctttgtttct agaaccttct ttccaaaatt aacaatcaat gaagatccag tatgtggatc
    29881 agcacattct aatcttattc cttattgggc agaaagaatg aataaaacaa aattgtcagc
    29941 ttatcaaatg tcatcaaggg gcggggatct agaatgtgaa ttaaaagaag atcgtgtgat
    30001 aataggagga aaagcagttt tgttttctaa aggtacctcg tttatttgat ttctttcaaa
    30061 tgaattaaaa ataaattggt ggagagtggc aaccttatag ctacaatggt tgttaaaaaa
    30121 agacgcaaac tttttcaatt taatgaaaag tttgcgtctc attttaatta ttggtgtttg
    30181 ttttttattc aatcaagagt aaaatgcccc atactaacta tcctcggatg acttcgattt
    30241 tataaccatc agggtcaatg acaaagtagt aagaaggcgc agtgccaggt aatcctttta
    30301 agtcagtaat ctcaagaccg gcagctttat gttgctcgtg taatttttct agatcatcag
    30361 ctgctatagc gatatgacca tagccgtctc ctagatcata cgcaccatga tcatagttgt
    30421 aagtcaactc taattcatag tcatccccag gaagtgtcaa ataaacaagt gtaaatttgt
    30481 tttctggaaa atcacggcgg cggctttctt caaaaccaaa tgccttttga taaaattcga
    30541 ttgatgcttc aagatctttc actcgaacac atgtgtgagc cattttcata gattcatctc
    30601 tccttttcct tttactcaat cataacaaaa agaagtaagg aaatagttga tttgtgcttg
    30661 ttcatgagaa atttctcatg taaaatggat agaaaataaa actgttaagg tggaaactat
    30721 tcatggataa accaattttt ggtgaaaaaa agcaatttgc tgattatcaa acccgttatg
    30781 cagcatatat tgtcatagaa agagatcagc agattgcagt aatcgaggct ccaaatggtg
    30841 ctttttttct tcctggcggg gaaattgaag gagaagagac gaaagaagaa gcgattgtcc
    30901 gtgagctgat agaagaaatg ggaattgtgg cagaaatttc ttcttatctg ggagaagctg
    30961 atgagtattt ttactcgaat tatcgtgcaa cttattatta taatccagga tatttctttg
    31021 ttgctgattc ttggaagcgt ataggagaac caactgaaaa gaccaataaa atttggtggg
    31081 tgagccctca agaagcgctt gtgaaattga aacgtggtag ccaccgatgg gcagtcgaga
    31141 gatggatcga aaaaaataaa aactgaaaga aagcgagtag atcagctttc tctcagtttt
    31201 tttatttatt acagtacata ttttttgatt gcttcagcta caccgtgctc gtcgtttgat
    31261 gaagtgatga catttgctgc ttctttgaca agagaaacgg catttcccat tgctactcct
    31321 agaccagcat attcaatcat ggataagtcg ttctcgttat ctccgatagc catgatttca
    31381 tcttgatcaa taccaaggtg ctgtgcgaga ttcgcaactg ccgcaccttt acttgcttct
    31441 ttatttagta cttcgaagta atagggcgca cttttgaccg ttgtgtattt atcacggaaa
    31501 gtttgcggta aacgcgcaat tgctgcatct aaaatttctg gttcgtcgat catcatcatt
    31561 ttgacgatgt tcatgtctgg tgtcatttct tcaggtgttc gatattttag cggcatcttg
    31621 actaaacttg cttcgtggat cgtataagcg ctgatatctc gattaggcgt gtagatcgct
    31681 ttgtcatcga tcgaatggag atgactgccg acacggcgtg ccatcacttc gatctctagg
    31741 aaatcttcat gtgtcagtcc gtgacgggcg atgattttac ctgatttcgt ttgttgtacg
    31801 agtgccccgt tataagtaat aacgtaatca ttatcttgaa aaaggttcaa ctcgtttaac
    31861 tgttcctgga cacctggtaa gggacggcca gtgcataaaa cgatcgaaac accttttgca
    31921 acagcagcgg caatcgcttc ttttacttct tttgttactt ctcttttctc atttaataaa
    31981 gtaccatcga tgtcgatagc gactagtttg atagacataa tttcctccaa tatattttaa
    32041 tttatttcaa tcaatgcgcc attacggata tggctggcaa attcttgata agtttcgtca
    32101 aataaatcat aatgatcacg caaggacgga tcaaccattt cttttggaaa gaaaaatcgc
    32161 tcatctcctt gttcttgtcc cgctaatgca gcaacaagct gactaacctg tgataattct
    32221 atcaaagttc cgtcatttcg caaaatctcg atttgtgtgc gatgtctgcc ttgttctggg
    32281 cgataaaaat cataaggtag atcgtagcta gaattcacag cagtgtaata aacaggatta
    32341 tatccgacct tttctactaa caaagtgagt tcttggacta attttgaatc ccggttgcca
    32401 gaaaaagtag cagatttcag tggtttacga ttcaaaaaac gtttagctaa atcatttaaa
    32461 ataggatcag cagattcaga ccattgagta aagtaagtgc ccaagacccc atcatcaagt
    32521 ttgaggtatt cttctaaagt aaagcttccg tcaagaaatg gcagaagtaa ttgagaatgg
    32581 agttcaaaac tgctggcatc ttcttgatac aactcgtgtg cgcgatgaag cagatgctcc
    32641 aaaattactt ccataccacg ggaaacagga tggaagtaca cttgcacata catttgatat
    32701 cggctaacga tataatcttc cactgcatgc atgccactca tggaaaaagc gatcccgcct
    32761 tcataaggac gaatcacacg taatattcgg gtaagatcaa atgttccata ttctgttcca
    32821 gtgaaataag catcccgaag caaatagtcc attcgatctg catcgatctg actagaaatc
    32881 atttggacaa cttgtgggtt ggggtaagtt ttttggatca cactagctac cttttcagga
    32941 aaaccttctt ctactcggtt aagaatctga tacacttctg tttctggaga agtgatgatc
    33001 tctacagtga tggcttcatg attcgtatgg aagatatgtt caaatgtatg agagtaagga
    33061 ccatgaccga catcatgcaa cagtgccgca cataaagtca ccagccgttc ctgatcatcc
    33121 cagcctccgt tgcctatttt ttcttttgaa aaatttcgag aaaagatatc gcagattcgt
    33181 cttgaaatct cataaacgcc taatgaatga gtaaaacggc tatgttctgc tccgtgaaaa
    33241 gtgaaagaag aagtgcctaa ttgtttaatg cgtcgtagac gttggacttc tttcgaattg
    33301 atcaaatcta agattacttg atgctggaca tggatatagt tatggactgg atcacgaaaa
    33361 actttttcca taggcagcat ttgatattta taaggggttg tcataatgta gctcctttat
    33421 gttattacgt tgttccattc tctggatttg gttgttccat tgctccagct ggatttcttt
    33481 ttttagccag tcggtagaat cctgtaaatc acttgcagaa tacagctttt caaaagcttt
    33541 tgcaaatgct tctttgactt cttttacaaa aaggggttgg tcaagcagtt cttcaagtgt
    33601 tgccattgta gcaggatcca ctgcaggata gccatttgta ccaaagtctt cttttaaagc
    33661 agattgataa aatcgcttca tgacttgccc gcgtttttcc tgattaccgt tgacacttaa
    33721 gtacatcata acagaaatcc catttttgat ccgacgctga gcgataccgg caaatttttt
    33781 gccatggatg cttaaatcat aagttccagg acaataagaa tgggtgattt cgaatgcttg
    33841 tatcggtgcc tttggaaaag cgtatttagt caataggagc atttgctcgt aagcgtcatc
    33901 tatggttatt ttttttgtgt cactttgggg gaaaatcaaa gaaatattca aaattccttc
    33961 atctgatacg atccctaatc cacctgaatt tctgatcact ggagcgtaac ctgcatcttt
    34021 cagtgtttgg atcccgtcac ttagaaaagg agttcgaaca tccttcatac ctaagatgac
    34081 tgtgcagtca tattgccaga aatgtaaaaa gatttgctgg tgttctttag aatattccgt
    34141 taatatatct gttaatgcaa aaggggaaaa gtcgtttttt tgataaatgt gctgatctag
    34201 tcgaatcgca gggttcattc aaccggctcc tttgtataaa ttcatatttt ctacaatatt
    34261 atactctaaa atcaaagagt tgttttggaa aaaaggtttt ctgtaaaaat ggacaagttg
    34321 gttgacaaaa caaaagaaac taggaacaat gtagctatga gaagtaaagg agaatgaata
    34381 atggaattat cacaaggaac accgatttca atcaaattgc gtacaaaagt aaaacagcaa
    34441 ggggaagtgc aagattttta ttttgatttg aaagggcaaa tggtgacaat tggcgatacg
    34501 ctgtacatac gttataaaga gattcaggag gagacgggcg atgagattcc tgtaacgatc
    34561 aaattagttc cagatggtca tgtacagctg atccgtgcag gagaaatgcg gatgcgtttg
    34621 aaatttggct ataaagaacg gttagaaaca tcctatcgaa caccatacgg tatgatccag
    34681 attgctactt ttacgaaaga actgcatgtt agtttgaaag accgtcccac aggagggaag
    34741 gttcgaatcg attatgatct gttcatggga ccggaaaaaa tcggcgaata ttatttgacc
    34801 ttggatttta cagcgtaaat ataaaagaaa agtttgattt tttgtgtctg attttgtatg
    34861 ataaggagta gactgtgaaa ggacgtgtcc tgattggaaa ttagtgtatt tgaaggtgca
    34921 aacaaaagcg aattatcaat gatcgaagta gcacacgcga tcttagaaca acgtggcgat
    34981 gtcatggatt tttctgattt agctaaccag atccaaaact atcttgggaa atcagacagt
    35041 gaaattcgtg attcactcgc acaattttat actgacttga atattgacgg cagctttatc
    35101 tcattagggg ataatcgttg gggcttgcgt tcatggtatg ctatcgattc gatcgatgaa
    35161 gaagtaaacc atggaatgga cgaagaggat gaagatactc cacgtcgccg taaacgcaag
    35221 aaagtcaacg cctttatcaa tgatgatgaa gacgcgatcg attacaatga cgatgaccca
    35281 gaagatacag atctgacaga agaagatgac gatgatctgt ttgatgacga tgatgatgaa
    35341 gatgaagaaa tcgctgctta taattctgac ttgcaagaaa tcggagcaga tgacccgaca
    35401 gatgatgaag aagacttgcc aggaaatatc gaagaagatt taagtatcat agacgaagac
    35461 gatgatgatg aagattatga agaggaagaa ttttctgact ctgaaaaaga ataaacatta
    35521 ttttcttacg agaggttgtg acagagacga tcagcctatc agaataagga ggtgtgacac
    35581 gat
//
LOCUS       DARBEG010000019        35308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000019 DARBEG010000000
VERSION     DARBEG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.019"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(222..707)
                     /locus_tag="SCK20_000947"
     CDS             complement(222..707)
                     /locus_tag="SCK20_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG1145033.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    797..1142
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1187..1828
                     /locus_tag="SCK20_000948"
     CDS             1187..1828
                     /locus_tag="SCK20_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1145034.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1847..2482
                     /locus_tag="SCK20_000949"
     CDS             1847..2482
                     /locus_tag="SCK20_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1145035.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2500..3315
                     /locus_tag="SCK20_000950"
     CDS             2500..3315
                     /locus_tag="SCK20_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1145036.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3634..5070
                     /locus_tag="SCK20_000951"
     CDS             3634..5070
                     /locus_tag="SCK20_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1145037.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5259..7706
                     /locus_tag="SCK20_000952"
     CDS             5259..7706
                     /locus_tag="SCK20_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1145038.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7936..9003
                     /gene="rlmN"
                     /locus_tag="SCK20_000953"
     CDS             7936..9003
                     /gene="rlmN"
                     /locus_tag="SCK20_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1145039.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9196..10299)
                     /locus_tag="SCK20_000954"
     CDS             complement(9196..10299)
                     /locus_tag="SCK20_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1145040.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10852..11400
                     /gene="aac(6')"
                     /locus_tag="SCK20_000955"
     CDS             10852..11400
                     /gene="aac(6')"
                     /locus_tag="SCK20_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1145041.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11946..13607
                     /locus_tag="SCK20_000956"
     CDS             11946..13607
                     /locus_tag="SCK20_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1145042.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13677..14024)
                     /locus_tag="SCK20_000957"
     CDS             complement(13677..14024)
                     /locus_tag="SCK20_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG1145043.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14108..15049
                     /locus_tag="SCK20_000958"
     CDS             14108..15049
                     /locus_tag="SCK20_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145044.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15050..16093
                     /locus_tag="SCK20_000959"
     CDS             15050..16093
                     /locus_tag="SCK20_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145045.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16099..17169
                     /locus_tag="SCK20_000960"
     CDS             16099..17169
                     /locus_tag="SCK20_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145046.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17159..18112
                     /locus_tag="SCK20_000961"
     CDS             17159..18112
                     /locus_tag="SCK20_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1145047.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18124..19134
                     /locus_tag="SCK20_000962"
     CDS             18124..19134
                     /locus_tag="SCK20_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1145048.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19419..20240)
                     /locus_tag="SCK20_000963"
     CDS             complement(19419..20240)
                     /locus_tag="SCK20_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1145049.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20337..20615)
                     /locus_tag="SCK20_000964"
     CDS             complement(20337..20615)
                     /locus_tag="SCK20_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145050.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20883..22892
                     /gene="metG"
                     /locus_tag="SCK20_000965"
     CDS             20883..22892
                     /gene="metG"
                     /locus_tag="SCK20_000965"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG1145051.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23371..23958)
                     /locus_tag="SCK20_000966"
     CDS             complement(23371..23958)
                     /locus_tag="SCK20_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145052.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24133..25455
                     /locus_tag="SCK20_000967"
     CDS             24133..25455
                     /locus_tag="SCK20_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1145053.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25610..26386
                     /locus_tag="SCK20_000968"
     CDS             25610..26386
                     /locus_tag="SCK20_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1145054.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26379..26948
                     /gene="rnmV"
                     /locus_tag="SCK20_000969"
     CDS             26379..26948
                     /gene="rnmV"
                     /locus_tag="SCK20_000969"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG1145055.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26941..27825
                     /gene="rsmA"
                     /locus_tag="SCK20_000970"
     CDS             26941..27825
                     /gene="rsmA"
                     /locus_tag="SCK20_000970"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG1145056.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28276..28905
                     /locus_tag="SCK20_000971"
     CDS             28276..28905
                     /locus_tag="SCK20_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145057.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29029..30138)
                     /gene="ugpC"
                     /locus_tag="SCK20_000972"
     CDS             complement(29029..30138)
                     /gene="ugpC"
                     /locus_tag="SCK20_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG1145058.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30347..30769
                     /gene="mgsA"
                     /locus_tag="SCK20_000973"
     CDS             30347..30769
                     /gene="mgsA"
                     /locus_tag="SCK20_000973"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG1145059.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31092..31628
                     /locus_tag="SCK20_000974"
     CDS             31092..31628
                     /locus_tag="SCK20_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1145060.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31668..33431)
                     /locus_tag="SCK20_000975"
     CDS             complement(31668..33431)
                     /locus_tag="SCK20_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145061.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33434..35149)
                     /locus_tag="SCK20_000976"
     CDS             complement(33434..35149)
                     /locus_tag="SCK20_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145062.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT        11491 a         6286 c         7310 g        10221 t
ORIGIN      
        1 ttccgattta taatcgaaaa aagaagcatt tttatatatg cttctttttt cgtagaatca
       61 ataaatatgg atttggctaa ctatggaaga atactcgtat tagttttcag cgttcatact
      121 agttgttaca ttgtttgatt ggccaaaaga atttctgata tattttgtgc taaataaaga
      181 aaaagacgct tgaattggga aataagaaaa ctactctatc attacaaatt ttcataaact
      241 ggcagatgtt ctaagtaaac aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta
      301 gtggtattga tatttggatg aaagcctacg gtatcttgac gatcaatagc agcatcgatc
      361 acgacttgga tttttttctc tttgtcgtgc atcaatgcta atggagtcac tgtacctgct
      421 ggaacgccta ggaggtcagg aagctgttct tcagaaacaa aagataagcg cttttctttt
      481 aattgttcag ctagttgttt taaatcggcg tgcttttcat caggcaagat gacaagatag
      541 atttgttttc cttttcttga tttcaataat agatttttta cttgcggacc aggtagggaa
      601 aaagggacat ttttgactga cgtaatcgca ggatgatcga ctttttgaaa agagataccc
      661 agttttttta gtagttcata agcttcttgt tcggttgcac gactcatcgc gcatcaccct
      721 ccttttttta ttagtgtatc ataaaaaaga gttgacaaat gaaaaataat catgtaaatt
      781 acacgtaaag ttaaaacaaa tgacatcgaa aagaagagta gccgttaggt gtttttagag
      841 agtctctggt tggtggaaag aggcagcagt taaccgtgaa acacatcttg gagtcgattt
      901 tctgaagcgt atagtaggaa aatccgggga gcccgttaca gctgcggtcg ttgctagatg
      961 accagataag gctggctttt ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta
     1021 acgtcctttg atcttcggat caaaggactt tttgttttca tctgaacggc aacgactgtg
     1081 tgaggttttt tctgcaagga aaaaataaac agaggtggaa ccacgtataa tacgtcctct
     1141 tggcatttgc caagaggatt tttttatttt aaaggaggaa aagaagatgg agtatttgat
     1201 tgaagttatg ccgcaattat tagtaggagc actcacaaca ttgaaattat ttttatttac
     1261 cttgattggc tcattaccat tagggattgt tttagcatta ggattgtctt tgcattttaa
     1321 gccgctacgc tacttgcttc aaacatatgt atgggttatg cggggaacac cgctgttgtt
     1381 gcaactgatt tttatttttt atgggttgcc gacgattggg atcgtttttg atcgtttcga
     1441 agcagcactc attgccttta tcttaaacta tgcagcttat tttgcagaga tctttcgtgg
     1501 agggatacaa tcgattcctc aaggtcaata tgaagcagcg caagttttgc aattaagtcc
     1561 ctttcaaacg gtgacgaaaa tcgtcatccc acaagtgaca aagattgttt taccttctgt
     1621 agggaatgaa gttatcaact tggtcaaaga ttcttccttg atctatatat taggtttagg
     1681 tgacgtaatg cgtgctggga aaatcgcgat ggaacgcgac gcgactttac tgcctttgct
     1741 aggcgtggca atcatctatc ttacattgac tggtattcta acaatcataa tgaaacaagt
     1801 tgaaggaaaa ttaaattatt accgctaact gataggagga agaaatatga ttgaattgaa
     1861 gaaagtgaac aaagcatttg gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc
     1921 agatgggaaa acattagcta tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg
     1981 tatcttggct ggcttagaaa gttctgacag cggagaattt tatttagacg gtgtaccgtt
     2041 caacccattc gagcttaaaa tgaaagaaca agttgtagga gtcgtattcc aagacttcca
     2101 gctatttcca catctgaata tttttgaaaa cattacctta gcaccaaaaa tggtattgaa
     2161 acagacaaaa gaagaataca ccaaaaaagc agaagaatta gcggcttcat tagggattag
     2221 tgatttattg aatatctatc cttatcagct ttctggcgga caaaaacagc gggtagcgat
     2281 tgccagagca ttggctatga atccacgggt attggcttat gatgaaccaa caagtgcttt
     2341 agatcctgaa ttacgccagc aagtcgagcg tttgatccta tcattgaaag aagatggggt
     2401 aacccaaatt attgtcaccc atgatttagt atttgcagaa aatgtcggtg atcaattact
     2461 aaaagtttcc ccaattcatt aagaaaggag aagatagaga tgaaaaaaac cagttttcta
     2521 ttcgctttga tcgcaggtct tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca
     2581 gataattggg cgactatcaa cgaagagaaa aaaatcgtta taggtttaga cgatacattt
     2641 gttcctatgg gatttagaga taaagacggg aagctcacag gatttgatat tgatttagcc
     2701 aaagcagtct ttgaacaata tgggattacg gtagactttc agcccattga ctggtcaatg
     2761 aaggaatttg aactgaacaa tggaacgatc gatttgatat ggaacggcta ttccaaaacg
     2821 ccggcaagag aaaagaaagt gcaatttaca aaaccttata tggaaaacga tcaagtgctg
     2881 atcacaccta aagaatcagg aatcacaaac tttggacaaa tgaataataa gagattaggt
     2941 gcgcaaaatg ggtcatctgg ttatgatgta tttacgaaac aaccagaagt attaaaagag
     3001 atagtcaaaa accaagatgc tgttttgtat gatagtttta atgaagcatt gatcgatctg
     3061 aaaagcagac gggtcgatgg tctgctgatg gataaagtat acgcagacta ttatttgaaa
     3121 caaagaaacg aactagaaga cttcaacata acaaaaggtt cttatgagag tgaggatttt
     3181 gcagttggtg cacgcaagtc agatagtaca ttagtgaaaa aaatcaatga agccctagat
     3241 caactagaga aaacaggtga attccaaaaa atatccgata aatggtttgg cgaagatgtt
     3301 acgccgagaa attaacaaat aggggactgt gacaaaagac gtgtcacagt ccctttttcc
     3361 gaataaacag tgttctcctg ccaactcctt gtgactcgga gctgaatggc aggctcacaa
     3421 cctcttggta aacggtgttc aaaagccagt ttttcggtat taagccaaat ttatgcaaaa
     3481 atgtagaaag ccattttcac aaaatttttc ttactactcg aaactaagcg actttttcac
     3541 aacctccttt acataatttt attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg
     3601 aacgattgaa ggttatagga aaggaagaga tgaatggaac acaaagaact tacctcattt
     3661 ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc aagtagaagg agcttggcaa
     3721 gaagatggga aaggagaatc agtctgggat cgatttgtac ggatacctgg aaagacattt
     3781 aaaggaacga acggtgattt ggcagtagac cactatcatc gttataaaga agatattgct
     3841 ttaatgaaag aacaaggtct gaaagcttac cgcttctctg tagcttggac acggattttt
     3901 cctaatggac gaggagaagt caatcaagct gggctcgctt tttatgagag attgatcgac
     3961 gaattgattg aaaacgatat tgaacctatg ttgactttat atcattggga tcttccacaa
     4021 gcattgcaag atgaatataa tggttgggaa tcacgtcaga tcattgaaga tttcactcat
     4081 tatgcagaaa cattatttga ggcattccgc gggaaagtcc attactggat tagtttgaat
     4141 gaacaaaata tcttcacttc attaggttat ttgttagcag cacatccgcc aggtgtgaca
     4201 gacccgaaac gaatgtatga agttaaccat attgctaatt tagcaaatgc atctgtcatc
     4261 aataaatttc acgagttaga aatgcctgga aaaatcggac caagctttgc gtatacgcca
     4321 aattatccaa ttgacagtaa tcctgaaaat gttctggctg ctgaaaatgc agaagacctg
     4381 atggccaatt attggatgga tgtctatatg tggggaaaat atccgattgc tgccatgagg
     4441 ttcttagaag aaaaaggatg ggcaccaacg attgaagctg gcgatgcaga attgctagaa
     4501 tctgctaaac cagattttct tgggatcaat tactatcaaa cggcgacgaa tgcattcaat
     4561 ccattaaacg gagttggtgc tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa
     4621 gaaactggag ttcctggaat gtataaaaaa gttgagaatc catttgtcga acgaacaaat
     4681 tgggactggg agattgaccc agaaggatta aggatcggac tgcgtcgtat cacaagccgt
     4741 taccgcatgc cggtactgat tacggaaaat ggcttaggag agtatgacaa gttgacagaa
     4801 gataaacaaa tccatgacga ctatcgaatc aattacttac aaagccacgt caaagccatc
     4861 aaagaagcaa tttcggatgg agcagaagtc ctaggctatt gcacctggtc ttatacagat
     4921 ttgcttagct ggctgaacgg ttatcaaaag cgatatggat ttgtatatgt tgatcaagac
     4981 gaaacacaag aaggatcttt ggaacgatat aagaaagaca gttattactg gtaccaaaaa
     5041 ctgattgaag aaaatggaga gaacctttag ttcacatact taaaacaaga aggtacggct
     5101 ggatatggcc gtgccttttc ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata
     5161 agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa actttagttc ccttactttt
     5221 ttttgtataa tgaaacttga tgaaatgagg gataataaat ggcacaaaag aaacggccta
     5281 ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc
     5341 tgacgattgg tgttgggttt gccttgattt tatttgctgt cttcggcttt ttaaaattag
     5401 gttttttagg aatattgatt gcaaacggct ttcggatcat tgcaggaaat acttaccaaa
     5461 tcctctgtct tttattagcc gtattaggat tttggattgt cattaagaat acagagtttt
     5521 caattggtaa aaaccgacgc tggtttggcg gcatactctt ttatttcggt atcttattat
     5581 tgttacatgc ccatttattt ggcaaactcc atactgggga accaaatatc atggggacga
     5641 catgggattt gttagccagt gatataaaac aaagtcaagt agacaataac gttggtggtg
     5701 ggatgattgg agcaatcctt tatcatttta cttacttctt gattgcacag cctggaagtt
     5761 atttagcagc agtgctatta ctggcaggag gagcattcct gttcagtaat ctggaaggct
     5821 atcagctgtt aaatggtatc caatcaattg gagaacgagt acaggaatta ttggaaggtg
     5881 atcctgccaa gcaagctcga aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag
     5941 aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat
     6001 atgagaagaa aaaagcggtg agccagcaaa gggaaaatac tgctgatgaa tggcaggaac
     6061 cagaaaatac agagccggag cagctaagtt ttgttccaat cgacagtttt caagaaaata
     6121 tccatcctgc gaatctagag aaaccagtac cagatacacc aaaacagacc aatacagcag
     6181 aaggtttttc agatgagttg ccagaggatg acggtacctc attagaattt gagattgaag
     6241 cagaacagga aaatcaagac tatgaattac cgacagttga tttgcttgat tctataccaa
     6301 ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa
     6361 ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc cagcttagga ccatccgtca
     6421 caaaattcga agtccagccg gctgtagggg taaaagtaag taaaattgtg aacttaacag
     6481 atgatatcgc gcttgcttta gcggcgaaag atgtccggat ggaagcaccg attccaggta
     6541 aatcactgat tggtattgaa gtaccaaatg gaaagatcag tatggtttcc tttagagaaa
     6601 tcatcgaagc acaaccaaac cacccagata aattactaga agtaccttta ggacgtgatg
     6661 tttctggacg ggtacaaacg gctgatcttt cgaagatgcc tcacttactc gtggctggtt
     6721 cgactggtag tgggaagtca gtagcaatta atgggattat cacaagtata ttaatgcgtg
     6781 ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt
     6841 acaacggcat acctcatctg ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag
     6901 ccttacaaaa agtagtacaa gaaatggaag aacgatatga aaaattcgcg gcaaccggag
     6961 taagaaatat ttctggttac aatgaatttg ttcaacagaa aaacttggaa aacggaacga
     7021 aacatccaac cttaccattc atcgtagtga tcgtagatga attagctgat ttgatgatgg
     7081 tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg
     7141 ggattcatat gatcctagct actcaacgtc caagtgtgga tgttatcaca ggaatcatca
     7201 aagcaaatgt accttccaga atggcatttg cggtatcaag cggtacagat tctcggacga
     7261 tcattgattc taatggggca gaaaagctct tagggcgagg agatatgcta ttcttgccaa
     7321 tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat ttctgaccac gaagtagaac
     7381 gggtcgttca atttgttaca gatcagcaag aagctcatta tgaagagaaa atgatgccga
     7441 cagatgaagt tgaaacgaca ggtgctccag aacagccaca agatgagctg tttgaagaag
     7501 caaaagcact tgtcgttgaa atgcaaacgg caagtatttc ccttctccaa agaagatttc
     7561 ggatcggtta caaccgagca gcacgtttag tagatgaact ggaagctcat ggagttgttg
     7621 gcccgtcaga aggaagcaag cctcggaaag tattcattga gcaggaagaa acatcagaaa
     7681 cgttgccgga cgataatgac atgtaaaact tgtgtgatga agggttgaaa aacgaataac
     7741 caataaaaag acaaggcatg atgataccaa aagaaaaaat ggtttttttc catcttttct
     7801 aatacggtat gatgtcttgt ctttttagtt tttgtgagat taaagttcta ttcgatttat
     7861 ttgtgaagta ttgcattaag gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa
     7921 aagagggaaa atactttgga aaaacagtcc atttatggct tgacgaatga agaattaatc
     7981 aattggttta tagaaaacgg cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg
     8041 tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc tatcgaaaca attgatcgaa
     8101 aaattatcgg agcattttat catcaatcct ctaaaacaga tggtcgtcca agaagcttcg
     8161 gatggtacag taaaatattt gtttgaactc cctgataaaa acatgattga gactgtactg
     8221 atgagacaag aatatggctt gtcagtttgt gtgacgacac aagtaggatg taatatcggc
     8281 tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg atctgactgc tggagaaatt
     8341 gttgcacaga tcatgatggt ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt
     8401 tcacatgtcg ttgtaatggg gatcggtgag ccatttgata attacgacaa tgtaatggac
     8461 tttttgcata tcatcaatga tgccaaagga ttggcaattg gtgctcggca cattacagtt
     8521 tccactagtg gattagcaca caaaatcaag gaattcgcta ataacggctt gcaagtcaat
     8581 ctggctattt ccttgcacgc gccgaataat gaggtgcgaa catcgatcat gcggatcaac
     8641 cgaagtttcc caattgagaa attgatggaa gctgtggatg agtatttaga gaaaacaaac
     8701 cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa atgatagacc agagcatgcg
     8761 caacaattag cagatctatt aaaagataag aaaaaattag catatgtcaa cttgattcca
     8821 tataatccag tcagtgaaca tgatcaatat tctcgcagtc caaaagctga tgtattgaga
     8881 ttttatgatg tattgaagaa aaacggcgta aactgtgtga tacgtaaaga acatggtaca
     8941 gatattgatg cagcttgcgg acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa
     9001 taatcgtatc ctactcgttt tttcaattac cataaaagga gcagttttct taattaagga
     9061 actgttcaac ttaaataaga gaaaagattc tatagcgaaa gctataaagc tttttttgac
     9121 aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg atgacagcct gcttgatggg
     9181 ggttttagca attatttaac tgattttctt atatccttgc tggctttctt gtgtagcatg
     9241 ataagtttct acgtcaagaa tggcctgttt aaatttttct cgggtgattt cataaggcac
     9301 atgtttaata tcatttgttg ccatggcttt atctaagatt gcttctaaat cggtcgtgac
     9361 attgagattc atgtcggtta acgatactgg aagttggttc tttttcataa acacatagat
     9421 tttatcaaac aaatcgaact gctgatccat catcaataag gtcagtacac cataactgac
     9481 agatgctccg tgaaggtatc cctccatttt ttctagaaca gtcgtaccgt aatagaatga
     9541 gtgtgccata gcagaattat agttattgtt aactaaaaca gaaaccaatc cggtagtaac
     9601 aatgatatcc gaaacgactt gttcaattgc atatgaggga cgatcgtcgg cactatcttt
     9661 caatgcttgc gtaccataag tcaagagacg atcaatgcac atttttccga tttggattcc
     9721 taatctatcc gtgtagccta gatcatcgcc acgagcagaa aaagtagatt cgacttcttt
     9781 ggataaagca tcgccaatac ctgcccataa gtatttatca ggtgcttcgg caataattgc
     9841 tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag aagagtccgt ccattacgcc
     9901 ttcttcgtta tagatgacac ataccgcagt gacgggcgaa cagttcgaac caatagtggg
     9961 aaaggtgaag actggtttag ctagtttttc accaatgact tttacagtat cgcaagcacg
    10021 gccaccaccg actgcaaaca ggacgtctgc tttttggaca ctttcatgct ggatcaactg
    10081 ttcaatattt ccataagttg aattaccccc gtaccagaga ctggcgacaa cctctatttc
    10141 ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa agtgcagtct tccctccaat
    10201 cagcactact tttgtcccat attctttgac gattccattt gctttttcat acgcatctgt
    10261 tccaatggta taatttggta agaaaacact ttgactcata ttcatttcct tctttcttcg
    10321 ttgaaacggg ttaaaatttt aaagagtgat cgatctgact gcggctgtag atggagtaga
    10381 ggtgaaccta agacaggata agtgatgaat aaggtggcat gaacaccttg aaatcatcaa
    10441 taagtatcct aaaaacgaac aagtacaaaa acattgaaaa cgacaataat gaatttttgt
    10501 tcctcttagt atttctaccg attccctttg cttttttgta attatctaaa aattctaaaa
    10561 attaagatta ttatatgaaa acaggtttta tttgtcaatt cataattggt tttttttaaa
    10621 gcaaaagata atctatatca tgatgaacaa acagagtttt ttcctccaat tgatggcttt
    10681 cctgatcttt cctattttga ataaagtgtt tctgtataat ttttttattt tataagtaag
    10741 ataaataatg aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt
    10801 tcgttttttc aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc
    10861 agtgaatttg accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg
    10921 acttggccgg aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca
    10981 gaacgaatcg cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc
    11041 cctcaatacg gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga
    11101 aagaaccaaa taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga
    11161 ggaatcacga tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg
    11221 gacctgtatg agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg
    11281 tatgaattct atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc
    11341 tgggacaaac cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa
    11401 taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat
    11461 tacttcttga atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc
    11521 gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa
    11581 atggctttct acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
    11641 cgtttattca gataagggac tgtgacaaga cttttgtcac agtttctttt tgtttattct
    11701 tttttatctt tgtatggcaa taatagacaa tataacgaat attatttaat ggcttttgga
    11761 agaccatgga aaaccttgta caaagctatt ttttctgaaa aattccttga aacctcacga
    11821 aaaaatcatt attttatgaa aaagacaaaa aaaagtgttg attttttaat aattattcat
    11881 taaactctta aacaacttca gaaaatatta attattctga aaattccaat aaaggggtac
    11941 gtaaaatgaa aaagaaattt acatttggtt tggttgcggt atgcggcctt gtacttgctg
    12001 gatgttatgg cggcagttca gccacaagca gtagttcagc aaaaagcggt tcagcagatg
    12061 gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg
    12121 caacagatac aatcagtttt acagcattga ataacgttta tgaaggaatt taccgtcttg
    12181 atgaagacag caaaccacag ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg
    12241 gattaactta caaaatcaaa ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta
    12301 cagcagcgga ttacgtttat ggctggcaac gaacagtaga cccagctaca gcttctgaat
    12361 atgcttacct gtatgcgcca gttgaaaatg cagaagacat tacagctgga aagaaagata
    12421 aatcagaact agggatcaaa gcagttggtg actatgaatt agaaatcaaa ttgacgaaac
    12481 aaacaccata tttccaatat ctgcttgctt tcccatcatt tttcccacaa aaacaatccg
    12541 tagtggaaga aaatggggat gcctatgcat ctgcaagtga taaagctgtt tataatggac
    12601 cattcacatt agaagatttt gatggaccag gaacagatac agaatggact tataagaaaa
    12661 atgatcatta ctgggacaaa gatacagtaa aactttctga aatcaaagtc agtgtagtaa
    12721 aagaatcttc tactgcattg aacttattca aagatggcca agcagatgac gtgatcttgt
    12781 ctggtgagtt agcacagcaa aacgcaaacg atccagctta tacttctgtt aaagaagcgc
    12841 ggacaagcta tatcgaattc aatcaacgtg aaaagaattc accattcaac aatgtgaacc
    12901 taagaaaagc tatttcttat tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg
    12961 acggatcagt tgtctctaca ggattgatcc catcagatat ggtaaaaagt ccaacaacga
    13021 acgaagactt tgcaaaagaa gctggaaaat tagtttctta tgacaaagat aaagcaaaag
    13081 aatattggga aaaagcgaaa aaagaattgg gcgttgattc tttagaattt gatttgatgg
    13141 cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa
    13201 acttggatgg cgtaacagtc aaaccaacac ctgttccatt ctctgtacgt cttgaccgct
    13261 caacttcggg tgattttgac gcagtactag gcggatgggc tgctgactat gcagacccaa
    13321 gcagctttac tgatttgttt gtcacaggta attcatacaa ccgtggtcgc tggagcaatg
    13381 cagaatatga cgaagcagtt aaagcagctt catcaacaga cgctggcgat gaacaagccc
    13441 gttgggatga tttccaaaaa gcaatcaaga tcatctctga tgatatgggt gtcgttccgg
    13501 tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat caaaggtatc gttcaccatg
    13561 cggcaggcgc ttcatgggat tacaaatgga catacgttga agaataatcg ttaataccgt
    13621 gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat
    13681 cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc
    13741 agccaaaagc tgtacatacc gtagctggca gacgagagcg aggaaaattc tggttttttc
    13801 tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa
    13861 gaaaagaccc ataaaaaacc gccgataatc agaaacggca atgaccataa gaatagatca
    13921 ttagataatt gaatgagtgt aaaggaatgg ttgatgagat tttttattaa aataaccaga
    13981 atcagtagaa aagctactgc gtaggggata gatttttttt tcatttgatt gctcctttat
    14041 ttttggttca ttatctaata atgtagcgaa acatctgtca gaagtcaaaa gctaggggga
    14101 aacattagtg aatagttata tcaaatatgt attaaaacgt gtctttttca tgatcatcac
    14161 gctttggtta attgcaacaa tcaccttttt cttgatgcaa ttgctgccgg gcacgccata
    14221 tacgaatcag gaacgtttga gtcctgaaac aatcgagatg ctaaataaac aagtcggttt
    14281 ggataaacca gttatcgtac aatatgggat ttatctttct aatttgcttc aaggagattt
    14341 tggaatttcc ttccaattta aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg
    14401 accatccttg cagttagggt tacaggcaat tatctttgga acagtcctag ggacgatttt
    14461 aggaacgatc tctgccatga agcaaaatac atgggcagat acatcttcta cactcgtagc
    14521 gatattaggt cgttccattc caaactttgt atttgctgtt ttattacaat atatctttgc
    14581 aatcaaatta caagttttgc caatcgcaaa atgggatggc tttgtgtaca caatcttacc
    14641 aacaattgcg ttggccatgt cgccgcttgc tgactctgct cggtttatcc gtacggaaat
    14701 ggtggaagta ctgcatagtg attatgtgga attagcaaaa gcaaaaggat tgagccgttg
    14761 ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt ccattgatga ctttattagg
    14821 accgttagct gtagcattga tgaccggatc actggtggta gaaaatatct ttgctattcc
    14881 tggtatcggg gaacaattcg ttaagtcaat cacaacaaat gattatccta cgatcatggc
    14941 tgtaacgatc ctttactcat ttatgctgat ttttgttatt ttgatcgtgg atctattgta
    15001 cggtctagtt gatccaagaa ttcgtgtttc agaagggagc cgaggataaa tggaaatgat
    15061 accaaagcgt tatgaaacag ttgcagatat ccctgcggat gaatttcttc cattacaaaa
    15121 aaataccgaa gaagaacgtg aacagattac tgcaccttct ttgaatttta tccaagattc
    15181 atggcgccgt ttaaagaaaa acaaagctgc cgttatttca atggttttac tattggtcat
    15241 cattttgatc tctgtgatca caatctttgt ttctcctcat gatccaactg cccaaaacgt
    15301 ggcttatatc aatttgcctc ctcgtattcc gggaatcaat atcgatggat tgaatggaaa
    15361 agcaatggtt ggtggtgaac ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt
    15421 aaatttcttt ttaggaacag acggtttggg acgtgatgta ttaagccgct tatttatggg
    15481 aatgagaatc tcgttgctga tcgcatttat tgctgcatta ctagatgtaa cgattggtgt
    15541 agcttacggg ttggtttctg gtttgttagg cggacgtgtc gataacgtga tgcaacgttt
    15601 tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg attttgatgt tggtcgtttt
    15661 tgaaccagga attttttcaa tcgtggctgc tatggccatc acaaactgga taccaatggc
    15721 acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa gaatatgttt tagctggatt
    15781 gacattaggt gaatcaagaa tgaaaattgc attcaaacat attttgccga atatctctag
    15841 tgtcattatt attcaaatga tgtttagtat tccatctgct attttcttcg aagccttttt
    15901 aagctttatc ggtttaggtc ttacgcctcc gtcagcttca ttaggaacga tgttgagcga
    15961 agggtacaag acattcctgt atctgcctta tctattatgg attccggcag taactttatc
    16021 ggttatcatg attggcttta acttacttgc tgatggattg cgtgatgcct ttgatcctaa
    16081 aatgaaagag tgaagacgat gacgaaaatt ttagaagtga aagacttaga aatctctttt
    16141 gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca attttcattt gaataaagga
    16201 gaaacattgg cgatcgttgg ggaatctgga agtggaaaat cagtgacgac cagaagcatc
    16261 atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc aaatcctctt taaaggtaac
    16321 gatatcgttg gaaaatcaga aagagagatg caaaaaatcc gtggtaaaga aatagcgatg
    16381 attttccaag atccaatgac ctctttagat ccgacgatgc ccattgggaa gcaagtcgct
    16441 gagtccctta gaaagcataa taaagtttca aaaaaagagg gacaaaaagc tgcattagaa
    16501 ttgcttaaat tagttggaat acccgaagct gagaaacgta tcaacagtta tccgcatcag
    16561 ttctcaggag gtcagcgtca acggatcgtg atcgcgattg cactggtatg ttatccggaa
    16621 atcctgattg ctgatgaacc tacaacagcg cttgatgtga ctattcaagc acagattttg
    16681 gagctgctga aagaaattca gacaaaaatc gatacatcaa ttatctttat cactcatgac
    16741 cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt
    16801 gaagttggaa catcggaaga aattttctac aacccgcaac atccctatac ttggggactg
    16861 cttggttcaa tgccgacatt agatagtgcg aacgatcgat tgtatgctat tcctggttct
    16921 ccaccggatt tattgaaccc gccaaaagga gacgcattct atccaagaaa tgaattcgcg
    16981 atgaaaatcg atgcagaaca agaaccacca ttttttgaac tatcaaaaac acacaaggca
    17041 gcaacttggc tacttgctcc gcaggcaccc aaagtgacac cgccagaaga aatccaacgt
    17101 cgctgggcta tctttaaaga gaaacaaaaa catacttacg gtcaaggagg aattgctgat
    17161 gccaaataaa ctacttgaag ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa
    17221 tgaagtacat gctgtaaatg acatcagctt tcacatttat gaaggagaaa cattcggttt
    17281 agttggagaa tctggaagcg gaaaatccac tactggccgt acgatcattc gcttgaatga
    17341 acctactgat ggtgagatat tattcgacgg tcaagatata acgaagatca aagataaaaa
    17401 aggtttgacg aaattccggc atgatgttca gatgatcttt caagatccat acgcttcgtt
    17461 gaatcctcgt atgaaagtca gagatatcat tgccgaagga attgatgtta atggactagc
    17521 taaatcatct gaagagcgtg caaaaaaagt cgatgatcta ttgagaactg ttggattgaa
    17581 tccaagccac ggtactcgtt atcctcatga attttcaggt gggcaacgcc agcgtattgg
    17641 tattgcacga gcattggcag taaaaccgcg ttttatcatt tgtgatgaac caatttctgc
    17701 actagatgtg tcgatccaag cccaagtggt caatttgttg caagatctcc aaaaagaaca
    17761 tcaattgacc tatctattta ttgcacatga tttgtcgatg gttaaacata ttagtgaccg
    17821 aatcggtgta atgcataacg gcttgttatt agagatggga acaagtgatg aaatctataa
    17881 tcacggtgtt catccttata cagaaagttt gttatctgct attcctctac cagatccaga
    17941 ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa ccagatgacg gacaagtacg
    18001 tcaacttcgt gaaatcgcac cagaacattt tgtctatgca actgagcagg aagtcccata
    18061 ttacgcaaaa aaactaaaac gacaaaagga atctcttctg gttgctaact aggagggaca
    18121 tagatgctaa aagcttttaa attccgtatg tatccgacag aagaacaaaa acagcaattg
    18181 attcgtactt ttggatgtgc tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa
    18241 agttggcaac aaacaggagt agccaatttt tcgctcacac ccgctacatt aaaaaaagaa
    18301 tacccattct taaaagaagt agacagtctt gctttagcaa atgcacagct taatttggat
    18361 cgggcttttc gcaattattt caagggacgt gccagcttcc caaagcttaa aacgaaaaaa
    18421 agtatgtggc aatcttatac gaccaacaac caaacccgta cgatctatct ggaaaatggc
    18481 tacttaaagt taccaaaaca aaaagaacta atcaagataa atcagcatcg gccagttgaa
    18541 ggatcgattc gctccgctac gatttctgct cgatataatg aagaatttta tgtcgcacta
    18601 ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta aatggatcgg gatcgcctat
    18661 catccgaaga cattgatcca aacatctcga cctctagaag taactttacc aaaatttcac
    18721 cagacagaag aaaagctgca acatgcacag cggaaattga atgtgaaagt ccgctccgct
    18781 caccatagaa agatcaggtt agatcaggca agcaattatc agaaacaaaa gagaaaagta
    18841 atggatcttt atctgaaaca aaagaaccag cgagaagatt atcttgaaca attatctggg
    18901 aaattggtca aacaatatga ctacttattt gtagaatctt ttccaaaaga agaggcacat
    18961 gctgattttt ctattcacga ctggcataaa ttaataacaa aattgcgtta taagtcccaa
    19021 tggtataata aaaaatttct tttgatcaat accgacggag cagaagaaag caattccgta
    19081 agaaagagcc aagtagtaga aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg
    19141 aatgaaactg ataggagcgt tagggttagc cttgttaata aaaagtctta atgacttttg
    19201 tttctaggaa tttttctttt gaagaaaagc tcactaatta tgacaaacca ataaaacaat
    19261 agcagaaaaa gttcgtacga gctttttctg ctattgttaa ttttttattc agaaagttta
    19321 cgaatacatc aaaattctga aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt
    19381 ttcttgaatg attaggtctg aatcttttgc tcacaacctt attcatggtg ccccggcttg
    19441 agctgataga tttttgaagg acggccgatt cctacaggac gttcaccgat ctcatcgaaa
    19501 tattggagca tagctttctt aaaattggaa tgatcgatcg ttttataatc gatccctaag
    19561 aatttagcaa atacttttcg agcttccgta atcgtgaaat ctttacctaa aacttgtaga
    19621 acttgcggct cgtgttccat tttattgact acacggttga atgctttcaa gatgatttga
    19681 ctatggtcaa aagccaaagt atctttgcca gaagactctc cagtgactaa atcaagcgag
    19741 atagccactt ctccgctaga taaatggatc ttgttactat gacgttctag tgtgaaccag
    19801 cgtacttcct tggcatcatc tccggcgatt agcggttctt ctccaataaa ggctaagtag
    19861 ctgactgtga cgacccagcc tcgcggatct cgattaggcg tactgaatgt atgcagttgc
    19921 tcaatatttt cttttgaaat aaccacacca gtttcttcct ttgtttctct taagacgctt
    19981 tctccagttg actcatcctt ttggacaaag ccaccaggca atgcccatga atttcgataa
    20041 ggatgccctt ttctttggat caacaaaact ttcagctgat cttcttcttt gttatagcac
    20101 atcagtacga tatcgacagt caaagagggc ttgtcatatt ctggacgttc ctgtttttga
    20161 taccagttaa gaaattcttc ttcacttgct ttatgttcat aatataattt ttcttcttct
    20221 ttagaagcaa aacggttcat ctgaccacct ccattgacag taaaagtact ttttacctta
    20281 ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa aaatctaggt tatcatttaa
    20341 atatgaaaaa taaatggaag tatgagattg aataaaaaga aaagcagata aaacggcaat
    20401 actttatacc gctcttcacg aatggcaaag aagccacaaa taacgatata tacgtcaatg
    20461 ataaaacgca aaatccaagc tgctgtcata aaactgttca aatgcccatg gaggttaaaa
    20521 taaccataca tacgaatata aaaatagctg aatattaaat gtgctaatga aagaaaagca
    20581 atactaagtg taaaaaaaag atgtttcttc tgcatgattg ttccagtccc ttactaagat
    20641 agatagtctt tattatacca cgtgtgaggg gagaaataaa aagccgtttt ttagtagata
    20701 tgaaacaaaa caaaaacccg catttattca acctattcat ccttttaaag gatagattga
    20761 acgtagatag aagtcgttta tccagtcttt ttcattttga aaaaaagcta acagcatgct
    20821 ttttttatgg tatactagca aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc
    20881 ccgtggcaga aaaggaaact ttttatatca ctacgccaat ctattatcca agcggcaaac
    20941 ttcatatcgg caactcgtat actacgattg catgtgacgc aattgctcgt tataaacggt
    21001 tgatgggctt tgatgtgttt tatctcactg gagtggatga acatggtcaa aaaatcgaaa
    21061 caaaagcaga agaactaggt gtaaaaccgc aagaatatgt agataaaatg gcggctgatg
    21121 tcaagaaatt atggaaaacg cttgatatca gttatgataa attcattcgc acgacagatg
    21181 attaccataa agcagctgtt caaaaaatat ttgatcgttt attagaacag ggagatattt
    21241 acttaggtga atatgaaggc tggtactctg tttctgacga agaattcttt acagaaacac
    21301 aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg
    21361 gacacgaagt agaattggta aaagaagaat catacttctt ccgcatgagc aaatacgcag
    21421 accgtctatt ggaatactat gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa
    21481 acgaaatgat caacaacttt atcaaaccag gattagagga tcttgcagtt tcacgaacaa
    21541 cttttacttg gggagtacca gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg
    21601 atgcattatc caattatatt actgcactag gatatggctc cgaagatgaa agtctattcg
    21661 aaaaatactg gcctgcagat gttcagatgg tcggtaaaga aatcgttcgc tttcatacga
    21721 tctattggcc aatcatgctg atggcccttg atttgcctct acctaaaaaa gtatttgggc
    21781 atggctggct attgatgaaa gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc
    21841 ctgaaatgct tgtggaacgt tatggtttag atgcactccg ttattatttg cttcgtgctg
    21901 ttccgtttgg gtcagatggt gtctttacgc cagaagattt tgtttcccgt gtcaattatg
    21961 atctggcaaa tgatctaggg aacttattga atcgaacgat tgctatgatc aacaaatatt
    22021 gtgatggact cgttcctgac tatgcctcac ttgtcactcc atttgatagt gaactgtcta
    22081 cgacggctgc taacgttgtc ggacgttacc atgatgcaat ggaaaaaatg gaattcaata
    22141 cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag
    22201 aaccatgggt attagcaaaa gacgaagaaa agaaagctga attgaatagt gtcatgatcc
    22261 atttagctga atctttgcgt atcgcagcga tattacttca gccaatcatg acagaaacgc
    22321 cagagaagat cttcaatcaa ctaggattgg actctgaaac aatgaatctt gaaggattac
    22381 actttggcga atttccttca ggaacaaaag tagttgcaaa aggtacacca atcttcccac
    22441 gtttagatat ggaagaagaa gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa
    22501 caaacgaaga tacagtcaaa tgggatccag aagaaacaga acttgtttca acgaaagaaa
    22561 aacaaatcaa atttgatgta tttgaaaaag tagaactaaa agtagcagaa gtcatcaact
    22621 gtcaaaaagt agaaggggca gataagttgc tgcaattccg actagatgct ggagacagcc
    22681 aagaccgtca aatcttgtca ggaatcgctg aattttatcc tgatccaagc gaattgatcg
    22741 ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc
    22801 aaggaatgat cctttctgca gaagcaccag atggctcttt acaagtaatc gaagcaccaa
    22861 aatccatgcc aaatggctca gaaatcgcat agaaattcaa agggggtatg acactagttg
    22921 tgtcataccc cctttttccg aaatttgaga agcaattttc gtaaattcct tcttattact
    22981 tgaagccaat catataatac agaaacagag aactatccaa aataaatcct agaaaaagaa
    23041 gtacgagctt cgctgatttt tgttaattta gaataatact aataggaatc gtaaaaacag
    23101 aaaaaaagca taatgataaa gggtcgggca gattttaatt gattcatctg gtactctttt
    23161 ttattgtcct tacattcata aagtttgaat gcaatgactt tattacatct atatttatat
    23221 ctaacagtag cgttgatgtg ttaattataa gttctatcac tttatgttca attagtttgt
    23281 gctcttatat gtgtgattga aaatatagat aaaaaaacaa tcatcggctt tgacttcgat
    23341 gattgttttc tttattttgt aaaaatccta tcacttgatt ttatggtaac cttttatttt
    23401 tgtcttcaaa cggcttggta agagagcagt aagcagtgca aatgggatcg aaaagacata
    23461 ccagaaaatc gtacgtccga taccataatg ttgatagagc tttgtcaaca tcacgtgtaa
    23521 caagatcaac cctaaaagca aggagccagc tataaaccaa tgattatccc aatgcatgac
    23581 ttcactaagt gccatcactc caaaaggata gaaataaaaa tagatcccga ctaaggtcat
    23641 ccgccagttc tttgtaaatg ttccgatatt caataaaaag aaaatcgctg aaagcaaaat
    23701 accgataggc ggaaaaagcc caaccaaaaa ggaatacaac atcccccaca agatcatcac
    23761 aggacctttg atcaatgcaa cgattgcgat gaacaaagtc agcaatatta gtttttgtga
    23821 taacgttgca acagtcatta atccaaataa aattaatgtg aaccaatgcc aatgggactg
    23881 ctgttgttct ttacgggaaa aatcgacccg gttggataaa taatctgcta atttcggata
    23941 agaacgctta aaatccataa actgttcctc cttcgtggaa aaggtaggtt tagtatacga
    24001 aaaataaaaa agaaatacat ccgtcttaag agggagtttt tcactgaaag tggagaaaaa
    24061 agaatggtaa attaaaatat tctcagttat actgaaaatt ataccaaatt tcaaagaaga
    24121 aagaggatgg gaatgaaagg caaactaaaa cgagaaatca atctgtttgg cgcattagct
    24181 accgttatgg gaaccgtgat cggtgcaggc gtcttcttca aaactgctgc tgttactgct
    24241 agtactcagt ctacaagttt gacattgcta gcttggcttt tgggtggttt cttaacgatc
    24301 tgtgctggat tgacagtggc tgaattagct acagcaatcc cggaaactgg tggagctgta
    24361 aaatatattg aagctgcgta tggaaaattg cccagctttt tattaggatg ggcgcaaagc
    24421 ttgatttact ttcctgctaa tattgctgca ttatcgatta tctttgcgac acaattgacg
    24481 aacttattac aattatctac cgattatctt ttgttgattg cagtgatcac cgctgtttct
    24541 gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg
    24601 attgttaaat taataccaat tgctgtgatt gtcatatggg gattattgac cccaggacaa
    24661 ggaactgtcc agttatttcc aatcgaagct ggaaaagatg ttacttttgt cgaaggattg
    24721 agcagtgcgt tgttagcaac gttgtttgct tatgacggct ggttaggtgt cggtgcaatg
    24781 gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag caattattct tggtttgagt
    24841 tttgtaacag tcgtttatct attgatcaat tttgtttttt tgaaaacttt gccgattgat
    24901 catcttgccg gtaatttgaa tgcagcttct gaagcatcag acgtcatttt tggaggaatc
    24961 ggtggaaagc tggtcactat tgggatcttg atctcggtat acggtgcatt gaacggttat
    25021 acactaactg ggatccgcgt gccttatgcg atggctttag aagacgagct acctttcagt
    25081 aaacagctga caaagctttc aaagaagttt acggttcctt atgttcctgc ggttttccag
    25141 ttagcagtag cttgtattat gatgagcctt ggttctttcg actttttaac agacatgttg
    25201 atttttgtga tgtggctatt cagtttactg atttttatcg gtgtctttat tttaagaaaa
    25261 aaagcgccgg aattgccacg tccatataaa gtaccacttt atccaatcgt tccgattatt
    25321 gcgattctcg gtgctatttt cattttaggt atgacgatga tgacgcaaac gaaattagca
    25381 ttgatcggta taggtgttac cttgataggt atcccagtct attatcaaaa aaagaaaaaa
    25441 cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag
    25501 atcttcgtgc tttttctatt tcaataaaac aagttcttct attttgattt ttgctacttc
    25561 tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga gagataaaca tgatatttga
    25621 ttctcatacc catttaaatg ctgaacagtt caatgaagat attccagaaa cgattcagcg
    25681 agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga
    25741 aaagtctttg caattaagcc atgactatcc aaatatatac agtattatcg gctggcatcc
    25801 gacagaggct ggcagttata cgaaagaaat cgaaaataaa ttacaagaac aattgacttt
    25861 accaaaggtt gtagcattag gggaaattgg cttggattat cattggatgg aagacccgaa
    25921 agaagtccaa gataaagtat ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc
    25981 aatcagtatt catactagag aagcacttga agacacgtat caaatcctaa aagaagaaga
    26041 tgttcgagat atcggtggaa tcatgcatag tttcagtggt gattatgaat gggcaaaacg
    26101 atttcttgac ttagggatgc atatttcatt tagcggtgtc gtcactttca aaaaagcatt
    26161 agacgtccaa gaggctgcag caaatgtacc aatcgaacga ttacttgtgg aaacagatgc
    26221 cccttatctg gcaccagttc cttttagagg aaagcgaaat gaaccaggct atactcgtta
    26281 tacagtagaa aagattgctg aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac
    26341 atggacaaat gcgcaccgat tatttggtct aagcgaacat ggataagatg aagatccaag
    26401 agattatagt cgttgaagga aaagacgata caagaaggct gcgggaagta gttgatgcag
    26461 atacgataga aacaataggg tctgcaatca atgaagagat cctgatgcaa attgaacatg
    26521 cccaagagac tcgtggagtg atcgttttta ccgatcctga tttttcagga gaaaaaataa
    26581 gaaaaatcat tatggaagct gttcctcagg caaaacatgc ttttttgccg cgcacacaag
    26641 ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc aagtgatgct gctattttag
    26701 aagcattgaa aaaggtcgtg acgccagaca tttcccaaac ggctgtttct gaaatcacta
    26761 gacaagacct tttagactat ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa
    26821 aactaggtga cgagttgcgc atcggttaca cgaacggcaa acaattggaa aaacgtttga
    26881 agatgtttcg tattacgccg aaagaactcg cccaagcaat gaaaatagtg gaggaacaac
    26941 atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa aagagatttt attgaaacat
    27001 ggtttttcat ttaaaaaaag tttaggacaa aattttctga ctgaaccaaa tattttaagg
    27061 aaaatcgtag aaactgcgca aatagatgaa catacgaacg tgatcgaagt tggaccagga
    27121 atcggtgcat tgacggaaca attagccaag catgcaaaac aagtcgtagc atttgagatc
    27181 gatgatcgtt tgatccctgt attggctgat acgatgcaac cctatgacaa tgtgactatt
    27241 gtacatcaag atattttaaa gaccgatttg tcaacagctg ttagagaaac atttcatgag
    27301 gagcttccgt taaaagtcgt agcgaattta ccttactata ttacgacacc gatcatgatg
    27361 cattttcttg aatcagattt ggttgtggat gaactagtcg tcatgatgca aaaagaagta
    27421 gctgatcgta tctctgctga accaggaacg aaagcttatg gttcattatc gattgctgtt
    27481 caatattata tggaagccag tttagcattc atcgtaccaa aaacagtatt tgtccctcag
    27541 ccaaatgtag attcagcgat tttgaaactg acaagaagag atataccggc agtcgaagtg
    27601 acagacgaaa aagcattttt ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg
    27661 ctttggaata acttgcagca ttcgtatggc aaagatgacc agacaaaagc atggttagca
    27721 aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg gggagacttt gtcattacaa
    27781 gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga
    27841 cagaagttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagtt
    27901 cctcatgttt ttcgtttgtt tgggcttgtt accgcagatg caagcttttg aacaccgttt
    27961 aagaaagagg ttgtgggcct gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac
    28021 agcttctcat gtttttgagt tagttggtct tattacttga tgcaagcttt tgaccactat
    28081 tatgccgaac aaggagatgt gacacaaatt ttgtcacatc tcctattttt gtttcttgat
    28141 caaaaaattc ttatacttga tactcacact cattttgtat ggatttagaa aagaatatgt
    28201 gtcttgacag ttattagtaa gtgagttata ctaagtcgac atttatgtgt caagacatat
    28261 taggagagag aaaaaatgag aaaaaattca gttcgaaaac taacgatttc tgctttattg
    28321 attgggatgg gggtcattat cccaatggtc atgcctaaaa tcatgattcc accagcttcc
    28381 tttactttgg cgtctcatgt gccattattt attgcgatgt tttttacacc aggagttgca
    28441 gtggctgttg cgctaggtac gacatttggt ttctttttaa caacgccggt tatcatcgca
    28501 ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat
    28561 ccgagtatcg ttttaaaaaa tggacagttt acttttgcta attggcgttt ccaagggttc
    28621 aattttgtga tcggtgtgat tcattcttta gcagaaatgc tagtagtcag catcttttac
    28681 tttattggta atatgcctga aacatattat tctcaaggat atttttatac agtattcatc
    28741 ttgatgggga ttggtgggct tatccacagt ttagttgatt ataatattgc ttattttata
    28801 gcaggaacac tcagcaaata ttttgatatc cccgtattta cggcagcaaa aaaagaaaat
    28861 cggaaagagt cgattcaagg gtacgcgaag aaaacgacat attagacatg atctctcaaa
    28921 aaaaaaactc tcatctagta atggataata aatcctctaa gatgagagtt ttttgtccta
    28981 aaaagagctg caccaacatc ttctggcaca gctctaacag ggggcaattt atggaagttt
    29041 gatgacttgt tctgtttctt tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc
    29101 gactatttcg cctggtttat ggaaatctct tgcatctact tttgagatga actcagtaga
    29161 accagttttt gtgtaaagca tcgtttcagc tccaagtaat tcggaaacga caacttctga
    29221 tttcactacg gcttcaggtg aagcttcaag ggctacttgt tcactgtgga tgtcttctgg
    29281 acgaataccg aagataactt cttttccttc ataacctttt tcaactaata ctttgttttt
    29341 tccttcagga atacgaagct taagcccatg tccatctgtg ataacaccat ttttcaatgt
    29401 cacattaaag aagttcatgg caggagatcc aataaagccg gcaacgaaca tattgacggg
    29461 tgtatcataa acttctttcg gtgtaccgat ttgttggata aatccgtctt tcatgataac
    29521 gattcggtca gccatagtca ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt
    29581 ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg gctacacgta atttagcatc
    29641 taaatttgat aatggctcat ccattaagaa gacttttgca tcccgaacga ttgcccgtcc
    29701 caatgccaca cgttggcgtt gtccaccaga taaagcagct ggttttcgtt tcaagtattc
    29761 tgtcagacct aagatctctg ctgcattgtc cacacgtttt ttgatgtctg ctttatcata
    29821 tttacgtaat ttcaagccaa aagccatatt atcaaaaaca gtcatatgag gatacaatgc
    29881 gtagttttgg aaaaccatgg caatatcacg gtctttaggt gccacgtcat tcatgacttt
    29941 tccgccgata tttagttctc cttcagagat atcttccagt cccgcgatca ttcgcaaagt
    30001 agttgacttt ccacaaccgg aaggaccaac aaaaacgata aattcacggt ctttgattga
    30061 tagattaaaa tcagttacag aataattttc tgcattgtca tattttttat atacgttttt
    30121 cagagccatt tctaccataa tacgttccac actttctttt ttagttttat taactattta
    30181 aagtataaat gaaagcgtta actaacttca atgtcatgtt gaacaagaat tttttctttt
    30241 ttttgtcaat gtgctagttt taagctttaa taaatatcca taagaaataa aaactgatac
    30301 actggagtaa ataagtatgt taacaatatc aaaaggagtg tctcttatga aaatcgcttt
    30361 gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta gcaacagcgt ataaaacaat
    30421 attggaaaaa catgaacttt ttgctaccgg gacgactggg cagaggatca cggaagcaac
    30481 cggcttgccg atccatcggt ttaaatccgg accgttaggc ggggatcagc aaattggagc
    30541 catgatttcg gaggatcagt tagatttggt gatttttctt cgagacccgc tggctgcaca
    30601 gccccatgaa ccagatgtaa cagcacttat ccgtttatct gatgtctatg agattccgtt
    30661 agctacgaat atcggaacag ctgaggtgct actaagagga ctggaagccg gcttcctaga
    30721 ttttcgaaga atcgtccatg aaatagacaa gcatccactt tcattttgat ggataagcgt
    30781 atggaaaaat aacgtttaat gttcgcctat atgtagctat aaaagctaaa caaactgata
    30841 atatgcgata taatacgaac gtattggata atataaacaa taatattagt ttttcgtatt
    30901 tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa agatcccaaa aataatacag
    30961 agtagaaaat aaagcgttaa gtgctaagag gatgggatgt tgcctcttgg acgaaggacg
    31021 taatgttcgc ggttttattt tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg
    31081 agatgctaaa aatgaagaaa caccggttag acgcacgtat gattgctatt atgggcttat
    31141 tgattgcatt gatggtcact ttgtcccgac tggtagcaat cgagacccca tttattaaaa
    31201 tcagtgtcac atttattcca caagtgatca tgggcattct ttttggtcct ttttggtcag
    31261 ggataggcgc agttttagct gatttagtag gaatggccct cttctcgaaa tctgcattct
    31321 ttattggatt tacattgaat gcatttattg aaggagcaat ctacggattc tttttctatc
    31381 gaaaagagat cacttggaaa aatgccattc tggctacgct tagtgtcacg ctgatcatca
    31441 acttattttt gacaccatta tggcttgcgt tgatgtacca tgtgccattg ttcagctggg
    31501 tcgtttgggc gccccgcctg ttaaaaacag taatatggtt accgattcaa tcaattgcca
    31561 tttattacgt aggacgtagt attccttaca aaaaaatttt aagaagttta gcgatccacg
    31621 ctaaataaaa aacaagcagt cttcccatcc cagaagactg cttgttttta ttgtgctgtg
    31681 aattgtgctt gatagagccg tcggtaataa ccatcttttt gaagcagctc atcatgggtg
    31741 cccatttcga caatagctcc ctgatccatc actaatatct ggtccgcatt tttgattgtg
    31801 gacaggcgat gggcaatcac aaaactggtt tttccttcca tcattgctaa gaaggcttgt
    31861 tggatttttt tctctgtcaa tgtatcgact gagctggtag cttcatctaa gatcaacata
    31921 gggggattac tgatcatcgt tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc
    31981 tttaaccctt gaccgccaat cttagtatct aatttttccg gcaaacgttc tacaaattca
    32041 tacatataag aggcttttag ggcatcattg atttctgcgt cacttgcttc cggattccca
    32101 aaacgaagat tttctcgcaa tgtactgtca aatagccaag tatcttgtaa caccattcca
    32161 aaacttttac gtaatgtatc acgttggata gagcggatgt catggtcatc gactgtaatt
    32221 gttccttttg ttgtgtcata aaaacgcatc agcaaattaa caagtgtgga ttttccggcc
    32281 cctgtttttc ctactatagc aatggtttga cctggataag cagtaaaatt gaaatcttcg
    32341 atcaaacgct gcttggggtc ataagaaaaa gaaacatgat caaaagagac ttttcctttg
    32401 atatttttgg gttccaaggc ataggcatct ggcaaatcag gcttttcttc tggctgatcg
    32461 atcaaatcaa atgctcgttc tagaccagaa aaagcggttt gcagttgtgt cgtgattcca
    32521 gaaagttcga taaatggttt agaaaactga cttgagtaga tcgtaaagct ggaaatcaca
    32581 ccaacagtga ttgacgaacc actcttcaat gccaataggc cgccaactaa accaatcgat
    32641 aaataagcaa ggtgatctac aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt
    32701 gcccgttgtc ctttttcata tagatcagca tttagcagtt caaattgctg ttgactagct
    32761 tcttcttgct gaaaagcttt cacgattttc tggttaccga ttcgttcggt cacaaagccc
    32821 gagatatccc caataatcct ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg
    32881 atccaactca caaggaaaat aattggtgta gaacaaagaa cgaccaatgt cagaagtgga
    32941 cttagacgca tcataaaaat aaacgaaaga atgatgattg ccactccaga aaataattga
    33001 ttgaaggccg cagaaattgc aatggaaata ttgtccatat catttgtaaa tcgactgacg
    33061 atatttccgt gggagttttg atcataataa cgaagtggca atcgattcag atgagcaaat
    33121 gcatcttttc ttaattttgc aacggataag taagctaacc gattgcttaa cacttggatg
    33181 agccattgac tgactacgtt gattaaaacg ataccaccga atagaataag taaatggaac
    33241 agtcgattga agcggacctg tccttctcca atcatttgat cgatactttg accaatttgt
    33301 aacgtcatca caactgttgc aatcccattg atgattccta gtatcacagc tagccaaatg
    33361 tcttttgcat aggcagtaag gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt
    33421 tttactttca taactattct tcctcctgtg atgcgacgat ttcctgatat tctgctgatt
    33481 gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt accttgatcc atcactaaga
    33541 tatgatgtgc ttcttgaata gagcggacac gttgggagat aataatcact gtactgccaa
    33601 gataagtctg tagcgcctta cgcaaatcca agtctgtttg ataatccaac gcacttaaag
    33661 agtcatctaa aatcagtaat gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct
    33721 gtttttgtcc gccagagaaa tttttcccat tttccatgac ttttgtatct aaaccatttg
    33781 gaagctgacg gacaaattct tctgcctgag cgattttcag agcttcccaa cattcttttt
    33841 ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat agtcccggta aatagtacag
    33901 aagtttgggg aacaagagca atttgctgac ggagatgatc cagctgccaa gaacgaacat
    33961 ccattccgtc cattataact gtaccggaac tgacatcata gaaacgcgga atcaattgtg
    34021 tcaaagtcgt tttcccgctt ccggttggac caatgattcc aaggactgta tttcgcttca
    34081 gctgaaatgt aatattttgg agagaaagac cagaatcagg agtataacgg aagtcaacat
    34141 ggtcaaaagc gataattgct tcagaagaaa aatcgggagt agtactgacc tcagcttcaa
    34201 gaatcgaatt ttctgtgtct agcacctctt tcacccgatt tcctgaagct tcggcacgtg
    34261 tgaagatcac gacgaggttt gaaacaacga tcagtgccaa aagcatttga ttcatataat
    34321 tgatcaaagc aagaacttgt ccttgttgca agctaccaat atttactttg atcccgccaa
    34381 ctgtcagcaa acagatgatc ccgacattca tgatcaaggt tgtagcaggt gataataggg
    34441 cagagatatt ggaaacacgt aaataattgt tcgctagatc atttgtgctt ttatcgaact
    34501 tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac acgaacaccg ctaagattct
    34561 gactaacgtg ttcattgagc tgatctagtt tttcttgcac tttttggtag agaggaacgg
    34621 tttttttgat gataaaaaat aaaatgattg agaaaatagg caataatgct aagaaaaaca
    34681 gacctacttc ccagtcgatc acaaaagcca tgaccactga cccgatactt aaaaacggtg
    34741 ctctgatcaa taaacgaatg accattgcta aagctagctg aagctgattg atgtcgtttg
    34801 tcattcgggt gatcaaggta tctgttccaa aatgattcaa ctcgttgtga gagaaagtat
    34861 tgattttttt gattagctga ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag
    34921 catagtattg gcaaatgata gcacaaatca gaccaatcac agacatagcg accatccata
    34981 agcccatttc aatgattttt cctcggtcat tcatttttag tccattatcg atcaaggaag
    35041 ccatcattaa aggcaggaca agttcgaaga cagcttcaag aaatttgaag aacgggccca
    35101 aaatgatttg cttacgatag tcttttgcat aacgtaataa ctgaatcata tgattcctcc
    35161 tattatttat ttgtccacaa ttgaaaaaac aagtactacc acattgtagc ttaggaaatt
    35221 tagaatgaaa agagaacaag ccttttcata aatgaaagtt ttattatgaa gaagctttag
    35281 aaattgaata gactattttt tttaaaaa
//
LOCUS       DARBEG010000020        35258 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000020 DARBEG010000000
VERSION     DARBEG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.020"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            21..1448
                     /locus_tag="SCK20_000977"
     CDS             21..1448
                     /locus_tag="SCK20_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145063.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1540..1905)
                     /locus_tag="SCK20_000978"
     CDS             complement(1540..1905)
                     /locus_tag="SCK20_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1145064.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1902..2267)
                     /locus_tag="SCK20_000979"
     CDS             complement(1902..2267)
                     /locus_tag="SCK20_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1145065.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2459..2707)
                     /locus_tag="SCK20_000980"
     CDS             complement(2459..2707)
                     /locus_tag="SCK20_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145066.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2706..2834
                     /locus_tag="SCK20_000981"
     CDS             2706..2834
                     /locus_tag="SCK20_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG1145067.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2891..4585
                     /locus_tag="SCK20_000982"
     CDS             2891..4585
                     /locus_tag="SCK20_000982"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG1145068.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4589..4708
                     /locus_tag="SCK20_000983"
                     /pseudo
     CDS             4589..4708
                     /locus_tag="SCK20_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4685..4858
                     /locus_tag="SCK20_000984"
     CDS             4685..4858
                     /locus_tag="SCK20_000984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145069.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5236..6369
                     /locus_tag="SCK20_000985"
     CDS             5236..6369
                     /locus_tag="SCK20_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG1145070.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6400..6720
                     /locus_tag="SCK20_000986"
     CDS             6400..6720
                     /locus_tag="SCK20_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG1145071.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6749..7213)
                     /locus_tag="SCK20_000987"
     CDS             complement(6749..7213)
                     /locus_tag="SCK20_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145072.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7345..8598)
                     /locus_tag="SCK20_000988"
     CDS             complement(7345..8598)
                     /locus_tag="SCK20_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1145073.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8627..8944)
                     /locus_tag="SCK20_000989"
     CDS             complement(8627..8944)
                     /locus_tag="SCK20_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1145074.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8970..9290)
                     /locus_tag="SCK20_000990"
     CDS             complement(8970..9290)
                     /locus_tag="SCK20_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1145075.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9754..11169)
                     /locus_tag="SCK20_000991"
     CDS             complement(9754..11169)
                     /locus_tag="SCK20_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1145076.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11186..12502)
                     /locus_tag="SCK20_000992"
     CDS             complement(11186..12502)
                     /locus_tag="SCK20_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1145077.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12652..13350)
                     /locus_tag="SCK20_000993"
     CDS             complement(12652..13350)
                     /locus_tag="SCK20_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG1145078.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13639..14595)
                     /locus_tag="SCK20_000994"
     CDS             complement(13639..14595)
                     /locus_tag="SCK20_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1145079.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="SCK20_000995"
     CDS             complement(14708..15541)
                     /gene="msrA"
                     /locus_tag="SCK20_000995"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1145080.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15716..17350)
                     /locus_tag="SCK20_000996"
     CDS             complement(15716..17350)
                     /locus_tag="SCK20_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145081.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17337..19205)
                     /locus_tag="SCK20_000997"
     CDS             complement(17337..19205)
                     /locus_tag="SCK20_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145082.1"
                     /translation="MISIIWFLFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKD
                     NSSILKIELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLS
                     RIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLL
                     NICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVL
                     YYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIK
                     FVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNN
                     DITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPIS
                     LLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAK
                     VIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKY
                     KAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENK
                     IEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            19171..20808
                     /locus_tag="SCK20_000998"
     CDS             19171..20808
                     /locus_tag="SCK20_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145083.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(20953..21693)
                     /gene="truA"
                     /locus_tag="SCK20_000999"
     CDS             complement(20953..21693)
                     /gene="truA"
                     /locus_tag="SCK20_000999"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1145084.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(21884..22519)
                     /locus_tag="SCK20_001000"
     CDS             complement(21884..22519)
                     /locus_tag="SCK20_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1145085.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(22666..23907)
                     /gene="sstT"
                     /locus_tag="SCK20_001001"
     CDS             complement(22666..23907)
                     /gene="sstT"
                     /locus_tag="SCK20_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG1145086.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            24105..25103
                     /locus_tag="SCK20_001002"
     CDS             24105..25103
                     /locus_tag="SCK20_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145087.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            25116..27134
                     /locus_tag="SCK20_001003"
     CDS             25116..27134
                     /locus_tag="SCK20_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG1145088.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(27194..27910)
                     /locus_tag="SCK20_001004"
     CDS             complement(27194..27910)
                     /locus_tag="SCK20_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG1145089.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(28137..28679)
                     /locus_tag="SCK20_001005"
     CDS             complement(28137..28679)
                     /locus_tag="SCK20_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1145090.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(28684..28911)
                     /locus_tag="SCK20_001006"
     CDS             complement(28684..28911)
                     /locus_tag="SCK20_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1145091.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(28990..30870)
                     /locus_tag="SCK20_001007"
     CDS             complement(28990..30870)
                     /locus_tag="SCK20_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1145092.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(31059..31673)
                     /locus_tag="SCK20_001008"
     CDS             complement(31059..31673)
                     /locus_tag="SCK20_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145093.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            31815..32375
                     /locus_tag="SCK20_001009"
     CDS             31815..32375
                     /locus_tag="SCK20_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1145094.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(32680..32994)
                     /locus_tag="SCK20_001010"
                     /pseudo
     CDS             complement(32680..32994)
                     /locus_tag="SCK20_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(33248..34883)
                     /locus_tag="SCK20_001011"
                     /pseudo
     CDS             complement(33248..34883)
                     /locus_tag="SCK20_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11168 a         6833 c         5716 g        11541 t
ORIGIN      
        1 tacaaggcgg aggctctact atgattgaag aatatattga gaaaaatatt ttaagacagc
       61 tgtttttatg tgggcaattc tatgtaaata aagaagtcaa tctagaaaag ctctccaatc
      121 tcctgcatgt ttgcaaaacc actttactca atgatatcaa taacataaaa aaagaatttg
      181 aagaacagat cgcttataca catcgggaaa aggaccgtta cactttatat ttttcagaac
      241 atatacctcg atgcaagatc atgcagcaat tgtctcaaaa ttcgttgttt ttaaaaactt
      301 gtctgcttta tttggaagaa gatgaacctg attatcttca attaactgaa tgtgaattca
      361 tctctgtcag caaagcttat tcattaaaaa aacaagtatt agcttacttt aatgactgcg
      421 ggatcgaaat cgaccgctac tcccctcgat tcactgaaat ggaaagacgt ctgctgcttc
      481 ttaatgtgtc gtatcgttta ggcggtttta attcttggga gctacctgaa tcgttttttg
      541 aacgtgccga tcgtttcatt gaatccgtaa cggaaaatag tggcagattt tatgataaag
      601 aaaataaaga aatcttatcg attggttttg ctatcagttt cctacgccag caagtgtgtg
      661 ctgtgacaat agattcaaaa tttatcgaag aaatcaaaaa acgtcctatt tacaattatg
      721 tcgaatcagc atgggaaaac actgatttcc aaacctatta taaaaaagaa gaattcgcct
      781 ttattttgac actattcaat ctttgtaatt atggtttcca ttcttatcag ttgattgcgg
      841 aagactttca acagctacac caagtgttta ttgacaatac tcccgaaata aaagaactag
      901 ttgctacctt tgaatctcac ttcaatcaag aattattcgg aaaccaaccg ttcgaacgtg
      961 cgctgattca tctgatgcgt tcggcttggg ataactatca attattcatg ccagaaaaat
     1021 tttatctttt aaatgaggaa cagacaaatt tatataaaga agtccagaca attttttcca
     1081 gctggtccag ccagttacct tatgatctac ggttgaatcc taattgcatg cgggcttttg
     1141 tgatcgaatt gagcggaatt ctccgtctga caaaagaaca tctgactatc tatatcgtca
     1201 caaattcgga tgttcattat ctgatttatc gggaagcctt ggaagctgta actactttcg
     1261 attttcaagt tgcaccaaca atttattcaa gtatatctga tataaagaag tatgcgcagc
     1321 agtcctcaaa ccgggtatta tgtgaacgta cattatatac accagatgcg gtccaatatg
     1381 aaaatattat tcctatttcg atcaatacga tcgatcgagc tatcatttca gctgtgcaaa
     1441 acaaataagg ggctgtgaca aaaatcatgt cacagcctct tatttgttga agatgcccat
     1501 ttctgtaaca atctcttgat acacgtcagg ctcacaacct cacttatttt ggacatctag
     1561 gggtccgatc tctttattta aaaaataaga aatgatggtt cggataacta ccaacgccgc
     1621 tagttggaaa atatcctgca aggtaggttt gataatcgat tcgatgatcc cagctgaaac
     1681 aagaatttcc aaacttagaa gtatatagcc tcccaacatc tttttaatag cattgttctc
     1741 gcttactcgg tcgctttgca cttttgtatg gacaaataaa tatttcaaaa aatccttgat
     1801 tgatagacaa acaccccaaa ccaataaaca gatagcaaag atatcaagtc ccagtacaag
     1861 tttttcgaaa tatggaagaa gaaactgcat caactctgtc gtcatcctac ctctcctctt
     1921 ctttgttttt ctttgtcttc tatttcctga ttcaagaaat aagaaatgac tgttcgaatg
     1981 ataataataa gtgcaagctt caaaatatct gtccaagtcg gcttgataac tgactcgatg
     2041 atatcagcaa cgactagtac ttccaagctt aacaaaatat aacttgctag cagttttctt
     2101 gtatctgcat tacgagtatt acgttctgca tagtttttct tgacatgaga ccaatcaaaa
     2161 agtttgaata gaacaagaac gatcccgaca attaagatac cgatagaaca aatatttaaa
     2221 atagcaatga tccattctag aaattcaatt aaatttgagc gaaacatata cgctacctac
     2281 tttcttttaa tttgttccta ttcttttatt gtaaaagaaa gttaccttgt tcgtctattt
     2341 aaaagttgtt ctaatgataa cggttcgctt aaatttttct ttttcactct cccatttttc
     2401 tccctccatc cataaatcgt ggaagtatcc aaaacaacgt agcaagttaa aataaggctt
     2461 agtccgtcat cagcttttca aagaagcact gtctcggtgc tttttttgta cccaaatttc
     2521 taaaaataga accatacacc tcctcaaatc ttttcttctt acctacccta tttttattta
     2581 caggaaaatc agacacttct tcttttttgt caaaatttcc tttttcttct cgtcctatca
     2641 tttggcattc taataaaaac gaactatcct tggatataga aagagaaaac aaggaggcgt
     2701 ttttcatgag taaaaaatta atcaaagtcg gcatcggttt aggtttgctt gcccttggcg
     2761 ctgcatatct aggaaaaaag acaggactat tcgaagatga cagtcattta tatgatgaat
     2821 tcgaatcaat ctagcatcct atttagttga atcactacgg acatacttgg aagacattgg
     2881 aggaatcaca atgagaaaaa gtaaagcaat aatgattggt gccggtattg ccaatatggc
     2941 agcggctgtt tatttgattc aagaaggcaa atggaaaggg gatcagatca ccttttattc
     3001 attggatgat cacggttcaa atgatggtgc accgaccgac actgtagtgg atgaatattg
     3061 gaataaaaac catccattag aaaaccaaaa aggctatatt gctcgaggcg gtcgcatgct
     3121 taattaccgt acctatgtag atttgatgga tctgttaagt cgaatacctt ctgttactga
     3181 gccagggatg actgctgaag aagatacgag agattttgat gcaaaacacc gaacctttga
     3241 caaagcacgt ttgatggaag gtggcaaagg aatcattgat ggcggaaaac tgggattcaa
     3301 taataaagac cgtattcttc tgactaaact gattgctatg cctgattcag aagaagaaaa
     3361 actagacaat atcactatcg aagaatattt caaagatgat ccacactttt ttgaaacaaa
     3421 cttctggttt atgtgggaaa caacatttgc tttcagaaca cgtagttctg ctcaagaatt
     3481 gcgtcgctat atgcatcaaa tgatttatga attcactcaa atcgaacatc tagtgggcgt
     3541 caatcgaaca cgttacaatc aatacgaaag tattatgctt cctttgatcg agtacttgaa
     3601 agaacaagga tgcaaaatca ttttgaaccg ccgtgtcact gactgggaat tcaaagaaac
     3661 accgatgcaa gatgaaatca ctgtgactgg tttgaaaatt accaatacac tgactcaaga
     3721 agaagaatac gttgtggtcg atgaagatac agcagttatt ttcactaatg ggtccattac
     3781 agattcagct actttaggag attacgaaac accagctgta gaaaatatgg attatggtgc
     3841 agcttctagt ctctggaaaa aagccagtga acgtttctac aacttgggaa atcctgacaa
     3901 attctttgct gatcgagatg caagcgaatg ggtcagcttt accctgacga cgaaaaacca
     3961 tttgttgttg aatgaaatca cccgtatcac gactcaagaa ccaggaaatg ccttgaattc
     4021 atttatttca accacaccga tcacgccgtt aggccaaaaa gatgtcaata tgtctattgt
     4081 cgtgcatcat caaccgcatt ttacgtctca gaaaccaaat gaaacagtta tttggggtta
     4141 tttcctttat ccaagacgac gtggcgaatt tgtggataaa cagtatatta aaatgaatgg
     4201 aaaagaaatg ctagaagaac tgattggaca actttcaaaa gtagatcctg gaccagtgaa
     4261 tattcgtgaa aaagaaacag aaattttcga cagcgtaata aataatatcc ctgtctatat
     4321 gccttatgct tccgcgttgt tcaataatcg tgccaaaagc gatcgaccaa aagtcattcc
     4381 taaacattcg accaatctgg cctttacagg agaatttgtc gagcagcctt atcagatgat
     4441 ctttactgaa caaagtgctg tacgttctgg agaaatcgct gctttccatt ttgcgggtat
     4501 tcctatgtcc cgtcttgtca aaacaccgcg ttacgacaaa gatattccta ctctagctcg
     4561 tgccgcaaaa aaaatgtttg aataatcaag aggttgtgaa aaagctgccc tgcatcaagt
     4621 aataagaaca accaggctaa aacagcttct tatgtttttg atttggttgg acttattacc
     4681 gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg ccctgcatca
     4741 agtaataaga gcagccaagc taaaacagct tcttatgttt ttgacttggt tagacttatt
     4801 accgcagatg caagcttttg aacaccgttt accaagaggt tatgaaaaag gcgtttagtt
     4861 tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata aatttgactt
     4921 aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct gtttcttttt
     4981 gaaaacaact attttttcaa cgtttactct cttgccaatc caacatcatc taacggatac
     5041 aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc cgaacacaaa
     5101 tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc aaaaaggtaa
     5161 gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt tatgaatctg
     5221 aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat ggaaatcaga
     5281 aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat cacagcaaca
     5341 gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt ggaaaaaggg
     5401 tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca agttcaaaca
     5461 ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca ttgtcatttc
     5521 tgcctgaatc acatggaaag ccaatgtgat gaatcaaatc cagatcagtt atatggcggg
     5581 cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga atatctaaga
     5641 gttccttatg cagactctac ttcatttgtt gtaccagata atgatcttcc tgatgaaaaa
     5701 gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa cgcaggagta
     5761 aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt cgctcaaaaa
     5821 ttcgctgtta tggctggagc agagcgcgta atcgcagtag atccagtaca gcaccgtcta
     5881 gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac ggcacaagct
     5941 ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga ttgtgtagga
     6001 atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt acaaagcggc
     6061 acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac agtccaagta
     6121 actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt tatgcgggat
     6181 atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat ttacgcaatg
     6241 attgcagaag gactattcga tcctacacaa atcattactc attcgctgcc actatcagaa
     6301 gcagcacaag catatgagat ctttgacaaa aaagaagata aaaatataaa agttgttttg
     6361 aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat caaacgaaat
     6421 acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg tcgcttccgc
     6481 ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt aaccgggatt
     6541 ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata tggcacctat
     6601 ttttatttgg ctggtgctat tttcttgacg attcgtccta ttctcagaat cgtaaggaac
     6661 atttttattt acgacgaaaa aaaagcaatg aagagaacaa gcaaaaaaaa agaggcatga
     6721 gaatttttca aatctcatgc ctcttttttc accctatcgt atcactttga tacagacgag
     6781 cggtcgtccc actttcttcc ttgaccattg tcaaaaatgt ccactcgtat cgttcataaa
     6841 acgtggtatg atctgtaatt aagtaaagtt tttcgtaccc aagttgatgc atttcttttc
     6901 tgatttccgc taatatccgc ttggctatcc cctgcttacg cttttccggt tcaacaaaca
     6961 gggcacaaag atttggtgtc agatcagggc ggttatggaa gtcgttttca atgacgcccg
     7021 ctcctccgac gatggtttct ttttcatcta agaccacata ccattgagga atactttcct
     7081 tattagcgat tccagtacga atactttcct catacacaac agcaggaact ccccattttg
     7141 atgaaaacca attcgcgctt tgttttacta attctgggtg ttctcttaat ttgatccagt
     7201 tatatgtaac caatgtttaa acctccgact aaaataaaac gtttacaaaa attatacaag
     7261 tctttatgaa aattagcaat gacaacaata gtgatgtaaa aaaaaaaacg gaaaacaatc
     7321 acttgttctc cgttttttga ttaatcaagc atctaaagct tcttttcttc tttcttggat
     7381 cgtagctacc ttgacgaacg gtagatagat caaaatagaa agaaccaaat tgacagcggc
     7441 tagtacacca cctgcaatac tttttgttgc caatacaccg ccaataattg gtggagttac
     7501 ccatggaggc ataacggtag ctgctggcac taaacctgta gaagtcgcaa agtaagcaac
     7561 actaactaag accattggtg tcaaaataaa tggaatgaac atgatcgggt tcaacacgat
     7621 aggtaaccca aacatcgttg gttcattaat attgaagaca cctggcgcaa tcgacaagtt
     7681 tgttacaacc atatatggtt tgtttttacg tcctactaaa taaatagcta acagtaaacc
     7741 taaagttgca cctgttcctc ctaagttgac aaagctgtca aagaaaggtt tgttcacgat
     7801 aaacggtgcg ctatgtccag ctgcgattgc attaacgttt gcttcaatgg ctggcgcatt
     7861 gattgtttgc atcaaaggat cgaccatatt cgctccgtgt aaaccaaaga accataagaa
     7921 tggtgtgata aatgcaagca gtaaggcaga tggataagag tttgcaagtc ccataaatgg
     7981 ttcttggacc actgtataga aagaacctac gatatcatca acaccgaatg cagcaaaaat
     8041 tgcagcaact aaagcaaacg cagagatagt gatcattgct ggtagtaatg aagcaaatgc
     8101 ttttgcaact gccggtggca caccatcagg cattttgatg atcaattttt catttccgct
     8161 taaacgtaca aagatttctg ttgaaataat cccaacaata atagcaataa acaaaccgtt
     8221 tgagctcata cctaatgctc cgcctaaagc gaagaaagag cctaaagaaa tcacacctgc
     8281 ggaaacaccg tcttttcggt agccgttagc taaatgataa gcaagcaaga aagcaatcaa
     8341 gacagaaaga atggcaaatg taccgttcca agcagcagcc ccgaatcctt tccatgattc
     8401 accaccaaaa attgagttca tcaattcttg gaacccggga atcggtaagt tattgatcat
     8461 tactgctaat gctcctaaga tcattaacgg catactcacc ataaatgagt cccgaatcgc
     8521 aactaaatgc cgttgtgcac caatttttga tgctacaggt ataaaatgtt tctccataaa
     8581 tgcaacaaat ttatccataa atcttccctc ttttctttac tgtagattac gcgttcaatc
     8641 gacgatacag attaatgatc tcttttgcta aatctgtaaa agctatagat gtcatcagat
     8701 gatcttgact gtggaccgtc aaaagcgtga cttcaacgtg atttccttgt gcttcttgtg
     8761 tcagcatctc tgtttgagag tgatgtgctt ggactaaaga ttcctctgca tctttgattt
     8821 tttcatctgc taaagtgaaa tcaccttttt tcgcagcagc aatcgcttcc attgcatcgc
     8881 tttttgcatt tccgccaaac atgatcagac ccatgatggc ttctagattt ttttgatctt
     8941 ccatttattg tatcctccga taatttttat tatttgttta ttagtttttc tgcttggtcg
     9001 agtacttttt caccattcat catgccatag tcagccatat tgattacatc taaaggaatg
     9061 cctttaggtg ctagcttttc agcaaattga ttttgcataa agcgtacttg cggtcctaaa
     9121 agtaaaacgt ctacattttt agattctagt tgattatccg catctgaagc agatacagca
     9181 aagatgtctg cttccattcc ttttgcttct gctgcctttt gcatttttgt gactaggaga
     9241 cttgtactca ttccagctga acatacaagc atgattgttt tcttttccat tttaaacacg
     9301 ttcctttcta tacttacact tgtatataag caagtagcgt gccaacttta aaagcgcttt
     9361 aaaaagttga tatcatcaca tttaaggtct ataccaagtt gcatacacac taaaaaagaa
     9421 agcgcacact aaacacacaa attaattttt attacctttt ttcttttaac gtgcacaaaa
     9481 aaagttgtga cacaaaactt gtcacaactt ttttcaaata aacggtattc aaaagcttgc
     9541 atctgcggta ataagtccaa ccaagttaaa aacataagat gttgttttta ctttgctatt
     9601 cttattactt gatgcaggac agctttttca caacctctca ataaacggtg ttcaatcagc
     9661 tgaccctggg tattagctca aaacgctgga ttcacaacct cttttatttc aaataaccag
     9721 taaatacaat gaacatctac aaaaaacttc tatttattta tagatttcgt caggcaaatc
     9781 caattctctc ttatcaatca aagtctgata ccacgaagca gattttttca atgttctgct
     9841 tcgttcatcg ttcaattcga ttcgaactaa tccatatcga ttcttaaatg cattcattgg
     9901 tgacacacag tcagtgaatg cccataacat atagccttcg caatttgcgc cttcttctgt
     9961 tgctttgatc aagtaatgca aatgctgtcc gataaattcg atacggtaat catcttgcac
    10021 gattcctttc tcatccatat actgttcttc atattctctt cccataccgt tttctgttat
    10081 cagccaaggg atattatgat aattttcttt tagatacatg gctatatcat acatgatttt
    10141 cggatagatt tcccatcccc tggaattatt cattttcttc ccaggtaaat caaagacttc
    10201 ataatatcgt tcaggatgga aaggtgtctc ttcattccat tgataacgag gggcgcgaac
    10261 gcgtttagga taatattgat tgattccaag gaaatctact gtatgatgct gaatcgtttc
    10321 taaatcctct gttgcaggat cgaaataaat cgattgtttt tgacacaact ccaggaactc
    10381 ttctgtgtag tatcctttta ccatcggatc aaaaaatact cgattataaa acaaatcata
    10441 catataagca gctttttggt cttctggaga attggatcga gcatagacca tttcaggatt
    10501 taaaatacag ccaatccttc caccataatt tccgctgcgg taaatctcta ctgctttagc
    10561 cgttgctaaa actttatgat aattccacat catccacttt tttgtgtttt gttcatgcgg
    10621 ccagcgaaca gcatccaaat agcaacgcgt ttggggaacg attggttcgt tgaatgtaaa
    10681 ccagtttttt accctatcac cgtaacgatc aaaggcaatt tgagcatact tagcataaaa
    10741 atcaactact tttcttgaag accagccatc atatttttcc aaaagaaaag ctgggacttc
    10801 ataatgttcc aaacaaatca tcggttctac gtctgcttcg atcaaagagt cgatcatttc
    10861 acttatatgc tgagcatatt cttcatctac tatacagttc tcataatcag taaaaaaacg
    10921 agcccaattg atagaagtcc gataatgtgt cagatggatg gatttcatca gatccacatc
    10981 ctgctggtat tgttccataa aattagttgc tacggctggg ccatacccat tatgccaaac
    11041 aaaatgttca tttttatacc agctatctat aaacgaatcc tgtccttctt tttttccttt
    11101 ccaaccttcc gtttgccaag cagaagatgc agcaccaaga ataaaatttt ttggaatagt
    11161 aattttcatg atttcctcct gttttttaga tcccatcatt ttccacacga tttgctactt
    11221 ttacaaatgg cagatagatc aaaatagaaa caataataca aacaagttgc gtaataactg
    11281 caccgatatt tcctgctgtt gccagataag cattgacgat cggcggcatt ggccaaggga
    11341 ccattacaac tgctttcgcc gcaaatccta aagcggttgc aaaataacca atcgttccag
    11401 tgataagagg cgttaagata aacggaatag ctaaaattgg attcagcatg atcggcatac
    11461 caaaaataac tggttcattg atgttgaaga tcccaggagc aagggataac ttggtgattt
    11521 gtttcatatc ttcccgtttt ccaactaaca atactgcaat cagtaaaccg atcgtgactc
    11581 cagacccgcc catactcata tacatatccc aaaatggcat agtaataatg ttcggtactt
    11641 cttttccagc ttcaaaagct tccgtattta cagcaattgc cgctaaaagc aacggttccc
    11701 gtatcggctt caccatttga tttccatgga taccaatcac ccaaaagact tgcgcaataa
    11761 acatcaatag caaaataccc ggtaaccctt gtacgacacc ttctaatggg cgttgtacga
    11821 cattgtaaat gatatcataa aggtacattc ctgtaagttc tttgatgata aatcccgcag
    11881 ccgcgatacc cgatacagtc agaatcgtcg ggaataaagc agaaaaacta gcagaaacat
    11941 tcgttggcac ggtatcaggc attctcagct gtagtttctt ctgtttcctc aaccagcaat
    12001 aaagttcaat agaaaggatg gagaccaaca ttcccaaaaa taaccctttc gtatctgtat
    12061 attgtttagc caatacgttt tctactataa cagtctgatt ttcattcact aattcataaa
    12121 cagttggatt aacagataaa taagacatca ctgcgatgat accagggaat acaccatcca
    12181 ctttattcag cttggctaat tcgatcccta tcagaaaaac agcatagatg gtcaagaaac
    12241 ttagtgtgac atagctgatg gcagatgcga taggctttaa ttcttctaaa aaacgcaaga
    12301 aacttatctg tgctaagcca ttttccgagt caaagaccat tgctgaaaac aatgtgccaa
    12361 aagcacccgt aataattacc ggaatcaatg cagcaaatgc gttttttatc accatgatat
    12421 accttaaatt attgattctc gttgccacgt tccctaaaac attggctaat ttatccataa
    12481 aactcattcc agatcccctc attcctgctt cttctccttg acgtcatctc tttttgtcta
    12541 ttagtcagcg cttacatttc gatgaacaga aactatttac tcctcttttt taccaaatat
    12601 aaaacaaaaa ttcatctgat gtatttatca taaaatacat cagatgaact gtcaattagt
    12661 ttctttttct cctattattt tctgtaattc tctccgattt attgccatat ggacagtcat
    12721 tgcatcaaac gcagcaagtg cattccgcct ttcgatggcc tgaacgatct ctgtatgaat
    12781 atcgatcgtc tcgattttca gatgctggat atcataattt ttattaaaca aagcaattga
    12841 ttcattaatg attgggataa tatgcagcaa ggctacgttt ccgctagctt ctgcaatcat
    12901 cgtatgaaaa tcgatgtcta attgaacatg ttcttgtgtg ccttctcgaa aagaagtttc
    12961 aatcttttgc gttaattttt tcatttggac gatttgattc ttcgttgcat attccgctgc
    13021 caaacctgcc atcttaggct ccaataaata tctcagttca aataagtctc gtatcatccg
    13081 atcctgatct tcaatcaaag aaaaaccgag tgggtcattg gcaatccctc gcttatcact
    13141 gacaaatgtc cccgctcctt gccgaacttc aaggatattc ctggaagcta gagcccgcac
    13201 agcttctcgc accgtgcttc gccccacctc taaataatcg gacaattcat attcattcgg
    13261 cagtttatct cctacttgca actgcatttc ctttaaatat ctaaggatct tgtcagtagt
    13321 tacttctact agggttttat tttcttccaa accacatacc tccttttcat ctgatgtatt
    13381 ttttcgtaat tattataaca cgtttgaatc atctgatgat tttttgtata caaaattgat
    13441 taatccgttc atagacatac aaacgaagaa gatatttacc ataaaaaaga ctaggataaa
    13501 attcactcct agccttcgat catttttatt ttgaacgaca aaaaatatga tgttcaataa
    13561 cagacgatct tcattcgatt cttgatgtca tcttttgttt tttttaatat atggataaaa
    13621 agtttgatta catcgtttct attctagaat ttggtcgatt ctcaattctt cttctggtga
    13681 gaaatctaaa ttatccatca ttcgaacact ttcttgtaac tgacttaacc gacttgctcc
    13741 gaccagtaca cttgtaacag atttttgtcg caaattccat gcaagggcca tttgcgctaa
    13801 tgattgtcct cgtgatacgg cgattgtttg cagtgctttg attttttcca gagttgatcc
    13861 aacttgttct tctgaaagga atggtatttc cttacgatgt gctctagaat cttcaggaat
    13921 accatgtaga taacgatttg tcagcaaccc ttgagcaagc ggactgaacg tgattgcccc
    13981 tagcccttct tcttctaata ctggagatag atcgtcttct agtagtgcac gagaaaacat
    14041 attgtagcgc atctgatgaa taatgaacgg tgcttccttt ctttttaaaa tttcagtcat
    14101 tttttttgta tcttctccat tatagttgga tataccaata tataatgctt ttccttgtct
    14161 tacaagttgc atcaaagctt ctgctgtttc ttccaaaggt gtatcagggt ctgggcgatg
    14221 atgatagaag atgtcaacat aatctagccc cattctttgg agactttggt cacaacttga
    14281 aatgatggat ttacgagagc cccattcccc atatggccct tcccacatat gataacctgc
    14341 tttgctagaa ataatcagtt catcacggta ggcttgaaaa tcttctctaa aaatacgccc
    14401 aaagtttttc tctgcagctc cagcagggcc tccataatta ttcgcaaggt cgaaatgggt
    14461 aattcccatg tcgaatgcgc ctcttaaaat ctctctttga ttatggatag gatcataatc
    14521 accaaaattt tgccataatc cgagagataa aatcggtaat ttcaaacctg aatgaccgga
    14581 tttacgatat tccatttttt cataacgtgc actatctgca atatacaatt tttctcctcc
    14641 tcatcaaatc tttttaaaac gctttctttt ttctattttt agtttactac attttaaaga
    14701 aaaggagtta atcaaaatga ttttttcgta tatttttttg ccatttgttt tttactcgtt
    14761 gatagatcag aaattgatta cgtgcttgct ggattttttt ataacgtttc ggttgttttt
    14821 tgtaaaattg ctgatgatag ttttctgctg gccaaaacgt accggctggt tcgatttttg
    14881 tgacgattgg ttggtggaaa gttcctgaac tgtctagttt ctgttttgat tgttccgcta
    14941 attctttttg tcgttcgact tgataaaaaa tcactggacg atattgcttc cctctatctt
    15001 gaaattgtcc aaatgtatct gtgggatcag taatttgcca atagattgtc agcaactcgc
    15061 tataacttat ttctcttgtg tcataaatga tctccacagc ttccacatga cctgtgtaac
    15121 caccacttac ttgatcataa tttggttttt caacatgtcc gcccgtgtat cctgacaaaa
    15181 cagataggat cccttttttc gattcaaacg gctcgaccat acaccagaaa catccccctg
    15241 caaatactgc acgttcttga tagtcagcat cttgcatggc atgcttggaa aagtcatatt
    15301 tttgctcctt atcaaatcct tccgtaatca ataaataaaa gtccattaca tcaggcgtca
    15361 gattcattcg gatagctaat ggacgtagtg ttgcttctag ttttgataac tgttctttaa
    15421 cggatatatt ttctcttatg tcattctttg tctgaatcaa tacttttcgt tcccaatctc
    15481 gtgtattcgg attcaaaatc agattataaa gtgtttgaat gaatatttgt tcctcatcca
    15541 tagattctcc tcctttccga agaagacaag ctagtgactt tagctgtaat ttatcataaa
    15601 gtgaggaaat aagcgattac ttcatccccc tacagatttt tcgtataaaa aagagagaat
    15661 ccttatcgac agattctcct ttttaatcaa tttattttat aaatgaaaag ttaatttacc
    15721 ttttatggaa aactatttta aaaaataaac ataaatatgc agttaatgta acaagataag
    15781 aaataatagt cgttactgta taacttgtgc ttactttaat atcagcaact cctttgactc
    15841 cgcttatttg taccaatcca ttagcattca cctgctcctt tacaggttga tgatttattt
    15901 ctacattcgt tcctaaataa gcatatacag gaagtgtgat tacttggctc ttctcattag
    15961 ttactctata tatgatagaa ctaccagttg ttttataagg aaggtttacc cattgactgc
    16021 caatctttgc ttcgtgtttt attatatgct ccttattctc caatgaagcg ataggagaat
    16081 aatcatataa aaaataatcc attcctttag tggtatacgc ctctactgtt tctttgttta
    16141 caacaatatg tcccggctga gaaattattt tttgattgaa atttttttta ctagaaacag
    16201 ttaaagtgat taataacaaa acagtcataa ataaataaaa tagttctctt gtcttattgt
    16261 atcttctatt tttgaaaaat atcatcgtta atatcagaga agagaatatt atttgcaagc
    16321 ttaagatccg ccaaggaaat tgtaacaatt tggctggcgt attttgcaga agataccatg
    16381 gcaccaaact agttgtagct agccaagtca aacacgtacc ataataaatc tttttttcta
    16441 tgtttgttaa ttttttaaat aaaatcaggg gaatacataa agataacaga ccaactagtc
    16501 ccattgagta agtagttaag tcgttgttca tagcttcagt cagactatcg aacacattag
    16561 atgcatgagc atataaattt gtcacgctag gtttgttgat tttttggtag agcatttgtt
    16621 caaacatcgg ataccaaaaa gcactcgtca gaaccaatgt cattcctgcc gcactaaata
    16681 attttttaaa tatagcaata aaatctgcat tcttgaaacc tttagaaacc aaacgaataa
    16741 aaataaaaat gataattaat gctgtactca ttaatgttga tagtacatgg ctgtacacta
    16801 gcaaactcat actcaatgct agctttacag acggatactt ttcttctttt ccagagatga
    16861 tttgataagc ataaaataat acagggataa caagtgttat tgcgatagct tcggcaattg
    16921 cggaacgata gtagatgtct actgtccgat aagcagagaa aatatagaga caactaaata
    16981 aaaatgccgc ttttttttgt tttaaaaacc gctcgccata atgataagct acgagtgttg
    17041 tacacaagtt caataaccaa acgtaaataa tatagcttac aatcaaattt tttgtcatcc
    17101 aatataacat tacagctgga aaaaaagtca gaaagggata aaaataattt acaccatatc
    17161 cagtataatt gaatgtctca taattaatcg gactggtaaa aatagtcgat aaccccttga
    17221 ttcgagataa gtgaaaagaa agatctccag tggtataaat actctgtgta tttttgaaaa
    17281 agatgaaaga atatatacta ctaactataa ttaatccact caatattact acttttttat
    17341 ttattttcat tcttcttgtt tatctccttt ttagtcacat atcgagaatc tttcaactta
    17401 ttaggtgcaa atcgtttaaa acttgaatac accactaaac aataagtcag aatactgatc
    17461 cataaagcca tgagagcagt tggagtatat tttgttgtaa tttctatctt attttctcca
    17521 gcatttacat ccacattcac taagccatgt tttttataag agtcatttac gactttaccg
    17581 tttactttta cctgtgtacc taaataagca tatactggta ctgtcacttt ttgctttttg
    17641 ttgcttgcca ctgtgtaagt aatgttatta tctgcagctt tatacttccc tttttcccac
    17701 gattctccta cttttacttc atgtttttct aagtgaggcc tttcttttat cgcttctgtt
    17761 ggggcgtaat cgtataatcc tcccatatct gatgttgtat aacgtgggag tgtttcttca
    17821 ttgataacaa tatgaccgtg ttttgattgg ataacttttg cgtaattttc ttttgctgct
    17881 atagacgtga cgagtagaaa caccacagta gcgccaatga ttaaccatga atatttttta
    17941 ttcatagtct ttttcataaa aacaagcgtt atgatcattg aagaaaaaaa gatttggata
    18001 cctaagatac gccaaggaaa ctggagtaag cttatcggtg ttttttgaaa caaaaaccat
    18061 ggaaataaat tagttacgca tacccatgta gaaactacgc caacatagat gattttttct
    18121 tttttctcca tttctttcag tatgaataaa ggaaaaatca acgcaaccaa tcctaataac
    18181 cccatagtgt atgttgtaat atcgttattt aacgcttgaa tgatactatc tgaaatagaa
    18241 agtgcttcta gctgcaaatc tgttttgaag ggactgtgta tttcttgata attcatctgc
    18301 tgcaacatag gataccaaaa atagctagtt aatgcacaag tcaaaaaaac agcctttact
    18361 aaattcacga ccacaaattt tatatcgttt tttatttgct tgcttaaaaa caaacgaact
    18421 ataatcatac aagcaatcaa tgcactacac aatagtgtag ttaagacatg agtatacaca
    18481 agtagactta tactgacaga taaagcgatg actttttttt ctctattgaa tatcaatcca
    18541 taagcataat ataaaacagc tgggataatc gtaagagcga ttgcttctgc aattgcggat
    18601 cgataataga tatcaactgt tcgataagtt gcaaaagtat ataaacaact aaataaaaaa
    18661 gcagcatcta tctttttaaa aaacttttct ccataataaa aactaatcag aatcgtacaa
    18721 atatttaata accaaatata tagaacataa cttaatatta agttatttga aattccataa
    18781 aaaacgactg caggaattat tgtaagaaaa ggataaaaat agttcagtcc atctccataa
    18841 ttgttaaatg ttgtatagtt gattggccct tcaaaaatag aagataaccc tttgattcgt
    18901 gaaagatgaa aagataaatc acctgttgta taaatttctt gagcatttct cataaaattt
    18961 actgaatata aagtactaag taagataaga actactaaaa gaaatgcttt tttacttatt
    19021 ttcatagatt tgttgttctc cttttttaat tctattttca gtatactaga attgtctttt
    19081 tttaataagt tttgcgttaa atttttcata gcactagtat gatatactag aaaagaatca
    19141 atttttatta cttttaaata ggagaaaaaa atgaaaaaaa acaaaaacca aataatagat
    19201 atcattttta tgttatttct aagttttgct tacgtcatcc ctctattcat gtctaaggga
    19261 atctatcata gtgtcaatca ggatacttac tttcatttat ctcgaatcat aggactagat
    19321 aatgtatgga gttctccagt aaacttcaat aacttcgatc atcatggaac aatgatgaat
    19381 attttttatc catggcttac tctttatcca gcttttttat tttataaaat gatgggaaat
    19441 cttgttttaa gttataatat ctactatttt tttatcactt ttttaacaat ggtcgtttct
    19501 tatttttcta tgaaacaaat aaaaaacaat cgttatattt cgctgttatt ttcgattatt
    19561 tatacatttt ccgcctatcg tgccattgac atattccatc gtgcttcatt gggagaagct
    19621 gtcgctttaa catttttgcc tttgatacta atggggtgtt acgaaattta tattagagat
    19681 taccaaaaat ggtactggtt gtctattggc atgacgctag tagtgtatac tcatttactt
    19741 tctgtcgcta tggtttctgt atttattgga ggaacgttgt ttctttcttt ttatttttgg
    19801 gaccaaaaaa tcgctagact cttaagtctt ttaaaagcta ccgtcctcac ctttttttta
    19861 agtgcgggat tcttaattcc ttttattcaa caatctagag cacaagaatt gaaagtccca
    19921 ctgggaaaag aattaagtgg tatggctcct agtgatatgc tgacacatat tttaaataat
    19981 aactataaca actatactat tggattattc ttatttctcg gattgattgg tgcttttatt
    20041 tttataaaaa aattaactgc tgatgattta tttattttct ttcttggtgt atttgttctt
    20101 ttttgttcaa ccaatttatt tccttggcaa ctttttaacc atacaccagt taagagtcta
    20161 caatttgtct ggcggttaaa tggtttttct tctttattta tagcctatac gatgagtata
    20221 gtgatctatt atattttttc tactaaaaat aattcatgga aaataatggt ctttacgttt
    20281 cttgcactaa tattgcatct ctcaggagta agcaattcta tccatgctaa caaagattca
    20341 ttaacactta tagcacctga agacgctgtt gctatagctg aaaattacaa tcatactgat
    20401 tatgcaaaca aagagtcaat ctatcatcct gatatgatta ataatgatca atatttacta
    20461 ggtaaccagg aaataaatcc aacaactcat tttatgagta acaaacttac aattgaatta
    20521 gataattcga ataataaaaa tactgtcttg acgacaccta tttatcgtta taaagggcaa
    20581 gtggcttcta ttaatggcaa actagtacaa acaaaattat ctaagtttgg cacaactgaa
    20641 ttaactattc ctccaggtat aaataaagtc gttattactt atcagtacac aaaactagca
    20701 attgcttcaa gatatttatc tatcgttact ttgattttat ttttacttta tcgatttact
    20761 ttttctaaac aaaaacaacc gagaagagaa gtacaacatt ctcactagat ttttatttaa
    20821 gaaagacttt ttagctgtac acataaacag gctaaaaagt ctttttttat atttctttaa
    20881 tttgcttcat acattattta acatcgatat ttatactgtg aaacatgcgt ttcatccatt
    20941 atcaaagtat ttttatcgat aataaacttc atccaaaaac agaccttgtg cagggacggt
    21001 ttctcctgca tgttgccgca ctttgctttc aaatatttca tcgattgttt ctatcggcat
    21061 ggcacctgtg ccgatttcta gtaatgtccc catgataatc cgcaccattt tatataagaa
    21121 tccgtctccg acaaaagtaa aatgaagcat ttctcccttt cgttcgatag ataattcatc
    21181 aatcgtacgc actgtggatt tttttgattt tttcaatgca gaaaaaccaa tgaaatcatg
    21241 tgtacccact aatttttcgc aagctgcatt cagcttgtcc atatccaact cttgagggaa
    21301 ataaaagctg tggtttcgct caaatgctga aggaatcacg ttgttccaaa cataatagct
    21361 gtattttttg cccaccgcat tatatcttga atggaagcgc tcaggcattt cttcgattct
    21421 tttgatgaca atatcccgcg gaaggtaacg attcataaag tcgatcattt ctgccagtcc
    21481 aatttctgtg tttgttttaa aatttgctac ttgtcctcgc gcatgggtgc cagcatctgt
    21541 tcttcctgat ccaataattt ctatctcttc atttgtcatc tgatgcaata ctttttcgat
    21601 ttttccttga atggttttat cgtcatctcc aagtctttgc catcctgaat atctttttcc
    21661 gtcatattcg attgtcaact tgatgtttct catattttag tggctcctct gtcttccatt
    21721 tggtcttctc tctacctttt taagattgta gcacaaaata tagctccttg cttctctaag
    21781 acagaagcta tacaagaaaa ctgtggaaat atatatttca gaaaaacaaa aaacagccac
    21841 cggatgaagt atccgctgac tgttcaattt gacggctcaa gttttactgt ttgagatgca
    21901 agatactcaa cctggcaatg ggcacataat taaagaaaaa actgattctt tggcaaaaac
    21961 ctattttatt cagtaatggc caagaattta tagaaggaat tcttgccagt ccataaatcg
    22021 ccgcaaaaag caaactgatc acagacagtc caaggtataa gtggcttacg tccatttgcc
    22081 cattttttcg atagatcaag aaaagaagaa ctggaaacaa taaaaaacca ctgtaaccta
    22141 aacctgatcc gatattatgg acccatgtcg agacattcca cgaagattca tgggtattga
    22201 tactgaacaa acctgtaaag atacaatcac ctataccata gaacgcgatg gctgctgtaa
    22261 ctagggtagc taacacacga gatgtactaa caaatgcagc atacaatgca ggcaatgaca
    22321 ggatgaaaaa gatccctgaa accacagacc aaataagaaa cgcttttctt acaggactgg
    22381 aaacctctcc aaatacactg atcacgctct tcatttgatc taattcagga taatacaatc
    22441 ctaatacgta cggagtcaaa aaatcactca gcacgcctaa taataaaaag aaaaaaccaa
    22501 atttacgtaa aaaatccatg atacgcctcc aataacaatt acctatgctt ttaattgtta
    22561 ttataaacga ctttatcttg atttaacaga aacctctatt tacacgaaca tctatataac
    22621 tcattaaaaa ctcgaatcat tttaaaaata ctaaatatct aacgttcaac gttcacttaa
    22681 tccatctaaa caatctttca atttgatttt ttccccactt cttttacgcg cgcctagctc
    22741 tcctattgca gtaaacaaca gatcacttga cgaatttaag gcagtttcca ttgaatcctg
    22801 gataacaccg ataataaatc caataccgac gatttgcatc gcgacatcat tagagatacc
    22861 aaacaaacta catgctaatg gaatcaataa cagtgaaccg cccgcaacac ctgaagctcc
    22921 acaagcagct aaagcagaca aaatacataa aaggactttc aaaacaaaag gtgcttcaat
    22981 ccctaaagaa tgtacagcag tcaacgtcat caaggaaatg gtaatcgcgg caccgcccat
    23041 attgatcgta gcacctaacg ggatcgatat ggaataagac tcttttgtca atttcaatct
    23101 ttcagccaac atcatgttta tcgggatatt tgcggcagaa cttcgggtaa agaaagcggg
    23161 aagtgcgctt tctttcaaac aaaagaaaat caatggatag ggattttctt taataaacaa
    23221 aaacgtgagg aatgggtaca caactaatgc gacaaacagc atcgtaccga tcaaaacaag
    23281 taaaagctgc agatactctg ccaatccagc gattccagtc gtagcaattg attgataaac
    23341 caatcctaaa atccctatcg gagcaaatgc aataatcact tgtacgactt gtgacaacgc
    23401 tatagatatc tgatccacca actcttttgt cgattctgaa cgtgctcgaa aagctagtcc
    23461 caacaataca gcccagaata agacgccaag gtaattcgca tcaataagag attgaacagg
    23521 attttgtgtg acattcgtca acatagtaga taaaacactt tccaaacttt gcggtgcttt
    23581 ttcagatact gtttcttgta aagtaatagg aacagtaaaa attgaactta caataactgc
    23641 tacaagtgca gaaagcaatg ttcccactaa atataagatc aaaataggct tcacgaatgt
    23701 cttattccca attttatgct tggcaattga cgccatcgtc aagaaaaaaa ccagaatagg
    23761 tgcgattgcc ttcaagcaac tgacaaacaa cgttcccaat attgaaataa atgtccatgt
    23821 ggggaaaaag ataccaaaca atattccgac taaaatacca acaacaatac gttgaactaa
    23881 cgacatatcc aaaaacttct ctaacaaatg accacccttt cacaatttta acaatcattg
    23941 tatcatttta tgcaaagaaa ctaaaggtat aattttctaa aaattctaac aatacaaaca
    24001 accatttctt gctaattttg tactttttct gtctacacgt tgttttttga gatattctaa
    24061 gttaggatgt tattaatggg caacttatca ggaaacgttt tcttatgttt ttgtccaatt
    24121 tttttgtgag gtgtttattc ttgaaaaaaa taaagaagga gagttatggc ttcctgacaa
    24181 tctttgtcgc ttgtataatg atggttttac agcgtatatt gatttcagca gtcactattc
    24241 ctacatatgc aacaaaccct ggtcgattct acatctatac atttttacaa acttccgttg
    24301 ttattggtgc aaatttgatc ccactttaca tgggttacca atatcaaaaa atcgcccgtt
    24361 taaaagtttt acattattta agtcgttttg cctttatatt cctaatagcc acactagttt
    24421 gcaatatctt tttttacgta caagctggca gcttgaatat tcgtgattat tggcttatcc
    24481 taacacctat cagtcagaat tactttactt atgctgtttc ttgtactttg cttttttgta
    24541 gtattcctta tacagttgga tttttaaata aattatcgaa aacaacaatt aaatatctac
    24601 tattattttt aacaattgga ttagttggct gctctactct atttaataaa gacccgtttg
    24661 cattacagaa tggacagagt attttatgga ttttttattt atttttaatg ggttatggtt
    24721 tgaaagaatg gaactggaaa gaaaaggtcc gctataagct tcctcaagta tttatcagct
    24781 tgattgtttt attcggtttg attattttga tgacacaaat ttcactcatc atcagaggaa
    24841 atacagatac agcgctgagg ttttctaaac catattctct cttcgcaatg tattatactt
    24901 tctcttcgtt tttattttta gaaatgctat ctgataaatt aggtataaaa ggaagaggaa
    24961 attttcttgc tgtatttttg attgtcactc aagttatatg taatttccct ttagtttctt
    25021 atcgcgtatc tacatttatg aaacgtgaat ttccaaactc agggaaggca tggctgataa
    25081 atatcgggca gttttttatt tgatatttat tagcagtgtc cctcctagtt atctttttat
    25141 tttatataca aaaaatccgt ttcattcatc gtttcatcga atacttcgct tttgattcga
    25201 ttgaacaatt aactcagcgt tggaaaaaaa gaattcattg gctttttgtt cataaaagtt
    25261 attttttagt tgcagcattt ttttactgct ttaccttcgt gcagattttc gttttagaac
    25321 caaaagaagg ttggaaagaa actatccagg ttgccttaaa aatatttact cagcgacaag
    25381 cacccatgat cctgacaatc atcattatta tgggattttt cttcttacta ttgttgattt
    25441 ccaatcgttt ttggtacgcc ttgactgcta ccttgatcat taacttactg ctcactattt
    25501 cgacagtaat caaaatggaa atgcgggaag agcctgtctt tccttcagat ttaaaaatgc
    25561 tgaccggatt gtcagaacta ttatcgatgg ttagcccagt cttattaatc gtgggcggat
    25621 tgatccttct cctattattg attactagta ttattgtaca acgtagacta caaaaacagt
    25681 attccttgaa gattcattgg aaacgtcgat ttatcagtat cgctgtatta ttaggatgtt
    25741 tttcgggtgt ctttttcatt aatcacaaaa attcgccttc tttcttgctg tttaatttat
    25801 ttaaagtaaa caagactttc tttaaccagc aaggcgccgt aaaagataac ggaccagtca
    25861 ttcaattcct aaacaatatc gacattaaaa tcatggaaga acctgctggc tattcgaaag
    25921 aaaaaattaa tggaataatg aaaaagtaca acacaaaggc agatgaaatc aaccaaacaa
    25981 gaagtgactg gttaaacaat cagacgatcg ttctcaattt aagtgaaagc ttcagtgatc
    26041 ctgcacgtgt ccctaatctg acagtaccta cagaccctat tccttacatc aaaagcatta
    26101 taacagaagg aacaggagga ttgatgttat ccgtcggtta tggtggcgga acagctaaca
    26161 tggaatggga aggactgaca ggcttgagta tcagtaacct gtctgcttcg ttagttacac
    26221 cttataccca gctagtggag cgtcagaaaa taagtccaaa tattaccgat ctattcgatg
    26281 aaaaaatagc catccatcct tttacagctg ctctttatcg cagaaaacaa gtgtttgata
    26341 aatttggttt tgacaggttc tattatttag acagtccgga taaattaact tatacggata
    26401 aaatagaaca aagcccacgc atatcggatg attcagcata caaagaaacc ttgaaagctt
    26461 tacaaagcaa tacggaaacg tcacaattca tccaattatc cactatgcaa aaccatatgc
    26521 cttatgatga ttactatagt gaattgaact acactgctga aggaactgct gtcattccta
    26581 atagaagaaa agaattacaa acttatatgc aaggccttca ctataccgat acagcagtaa
    26641 aaaaattcat ccaagaaatc gacaaaatcg ataaaccgat tacctttgtt ttttatggtg
    26701 accatttacc cgcaatctat agtgggaatg attcgaaaaa atatggatta gaacagcacc
    26761 agacagatta tttcatttat aacaacaagt atagtcgtga acaagcagct acgttaaata
    26821 aagaagttgt tgctcctaat aactttggtg ctatggctct tgagcatgcg aatatcaaag
    26881 taactcctta ctatgcgttg ttgacagaag tatctaatca tctacctgca ttaacaattg
    26941 atcctacaga aagcttgtct aatcgtttta atggaaaaca ggtctttgtt acagaggata
    27001 gtcaaattat ccaagaaaaa aatttaactg agaaacaaaa agaaattcta gaagattatc
    27061 gtctgataca atatgatctt gttgcaggta accagtacag tgcaacatgg gcgacacaaa
    27121 aaataaaaga ctaacaaata aactgagcaa ggggttgtga taaaagacat gtcacaaccc
    27181 cttgtttatc tcattaattt gcttgtccat aaacgatttg attaaagcca tttttcaatt
    27241 cattattcgc tgtctcgttg aagtaaagat aattatcggg attcccctga ttttgttgtg
    27301 tcaccaatac ttttggctga ttttgctgaa acccgaatag atagacgtgc tttttcagat
    27361 atggttctgt ctctgcatcg gaatagacgg ctgtgatttg atagtctgct tgacctgtcc
    27421 cactttctga ccattctgca gaaacaggca ctccaccaat agccatcttc cactccccgt
    27481 tcaaaatagc ttgagggact ttaagaccat ataagtccac actcatttga ttcgtataac
    27541 ttttgtattg ttgtcccatt gtttggcccc atttggtcat aaatgcctct aattgtgcgg
    27601 atttgtctgc attccacagt gtttgttcaa cttgttcgct gctttctgaa gaggaagatg
    27661 aagcctctac agaagaactt gaagtagatg tttgagttgt ttcagtcgtt ttggaagact
    27721 ctgacgaagt agaggtatct gctgtttctg tacttgctgc agttgacttg ctctctgtgc
    27781 tagttgttgt agtagttgca actgttgctt tcgctgtttc ttttgttgat tcttttttcg
    27841 attcattccc gttggtacaa ccagctaaca aaaaagctag accaattgtt actaaaatcc
    27901 tttttttcaa ttaaaaaact cctttcgatt actcccactt ctttattcta tcaaataagc
    27961 aatgtgattt tattgatttt tcgtattttc tcatcaatct ttcaaatctt ctaaaaactg
    28021 agaagcaccc gctgatgttg tctgtaatac atcagcgggt gcttctttta ttttaatctt
    28081 cgtgattttc caatttacct cacgctttat atttgtattt gaagaaacat agacttttag
    28141 tcatcatcgt cttcctcatc caaatcttgt cggacagttt ttccttggag tctttgtact
    28201 tggtagatga tttctttatt gaaacttaaa agttcaacaa gcaattgttg caagccatat
    28261 ttttcttctt tttcagctaa tttgattgcc cgtgtgataa agacattttg gaaatcaaaa
    28321 tcttttgcaa agttttccaa cattgttgag gctggtaaat atttgttttg accatgttcg
    28381 taaatttttc cataacgcat gacttcttct aaagtagacg gaacgatttc attttcaatc
    28441 actaactcat gagagatttt attgaaccat ctcgcttctt gatcagcata ttctgaaaaa
    28501 agggtactta attcttgcga agatgtaccc aaaacataca attgtgcttg gatcaatttc
    28561 atttcttcta atttaaaatt tgctaataca tgccccacca tggcgccagc agttggttta
    28621 tgatggtcga tatctgcttg ttttgtttct tgaacaaatt tttcttccac tatattcatt
    28681 gatttacgcc tcttttactt ttgctgattg gacttcataa cctaattttt ccacaactgc
    28741 agctaattgt tcagggtgga cttcatctgg atcaaagttt gctttgattt tgctcgcatt
    28801 aaacaaaact ttcgtattgc ttaccccagg ctgttgagaa accgcatgtt cgattttttg
    28861 catacagctt ggacaagcta gtgtatctaa ttgcataata atttttttca tcgtagttct
    28921 tccttccttt ttcttgatag tcttattcta gattacagct tccataaaaa aattgattct
    28981 tatcaagttt tacgattttt cttctttgct ttcgtgtcca aaatacaata aacgcatcgc
    29041 gttaaagata acgatcaaga tactagcttc gtgaacgaac atcccactag ccatgtaaat
    29101 aaacccggca aataagccgt tgagtaaaaa taagacgaca ccaatggcaa tcactacatt
    29161 ttcacgggta tttaaaacag ttttcttagc caagcggtag gcatgaacca acgattcaaa
    29221 attagattgc atcaagacaa cgtcactcgt ttcgatagca acatccgttc ctgatcccat
    29281 agcaatcccg atatcagccg cagccaaaga gggagaatca ttgattccat ctccaataaa
    29341 agcaacgcgc aatccttgtt cttggtattt tttcacaaat tgaacctttt cgtctggtaa
    29401 taattcagca tggacttcat caatgcctaa catatcagct acataatcag cagtcatctg
    29461 attatcacct gtcagcatca ctaactgttt tgctcctgct tgtttcaact tagctaattg
    29521 ttcagcgact tctggtcgaa tcacatctga cacacctatg atttgagtga tttttttgtc
    29581 gattgctaca ataatgaccg agctgcctgt ttgttgtaat tgatgtaaat cttgtacttg
    29641 ctttggtgtg agagaaagtt tgtgagcggc aatcaatttg gtgttgcccg cacaaactgt
    29701 atgaccatca atctcagctg tgattccttg tcccttgatt gtctgattat ctttgactgc
    29761 taattgttga aaatcaatgt ctttctcctg agcataatgg atcacggcgc gccccagagg
    29821 atgatcggat aacgtttcta atcgagccac taaagctaag ctgtcccctt catcctctgt
    29881 atagttcttg atagtggata cagcagtagt tcctttggtc aatgtaccag ttttatcaaa
    29941 gacaaaagta tcgattttgg aaaaagtatc cattgcttca ccgcctttga ctaagatgcc
    30001 ccgtttcgct ccattaccga ttcctgctac attcgaaaca ggcgcaccaa tgactaaagc
    30061 gccaggacaa ccaagaacca aaacagtgat tgctaaacga aaatcacgac taatcagacc
    30121 aacaaccaac gcaataagta aaacggttgg agtataatac tgagaaaatc gatcaatgaa
    30181 tttttccgca tgggatttgg tatcttgtgc ttcttctact aattcgataa ttttggcaaa
    30241 ggtcgtatca tctccgacct tagttgcttc aatcgtcacg tagccatctg ctaaaatcgt
    30301 tccagagaag acttcatctc cactgatttt gacgacttgt cgtgattcac cagtcactgc
    30361 ggcttcatcc gcataccctt tcccgtcaat gactcgtccg tccacaggaa tagctccacc
    30421 agtttttact aagacgatat cgccttcttc cacgtcatcg atttccactt cttcagtcgt
    30481 tccatcttct tggacaacac tagcagtaga tggggccatc tcggtcaaag ccttgattga
    30541 ctcccgcgtt ttggtcaatg ttttttgttc cagatagcta ccaaacaaaa acaagaatgt
    30601 aacgatagct gactccttgt attctccgat cataaaggca ccaagcacag caatagtaac
    30661 gagtaaatca attgagataa ctttgacttt cactgcctga tacgcatgga tcataatagg
    30721 tatgaccgca ataatagaag ctatggctag agcaatgtta tagaacaaga caaaaccagt
    30781 aaattttccg ataaaaccaa aaataatcaa cacagctgat aaggcggtga tatgatttcg
    30841 atgctcttgg atccactttt gtaatttcat ctttctccct cttttccttt cattcttacg
    30901 agactaagta tagatactta tccatcataa aaaattgacc actatcaagt ttagtcaaaa
    30961 aggcggtttt cttttggtag aatacactac aaaaaatagt tagcaagaag actcactaaa
    31021 agtgatcttg ccaactattg tcgtttctat cgaaattttc attgaatgat tttcctgtac
    31081 agcatacatc ggagatctat ttctttatct aagattttta tcttgctaat tttttccttt
    31141 agtctttcaa atccacgtcg ttcatagaat tgatacgtac agccactatc tgtaaaaagg
    31201 aatatttctt ttcctttctc tcttttttca aattctgtta gcaacttggt accgatgcct
    31261 tttgttttca aatcaggatt tgcagctaaa aaaatgatct ctccatctgg cgaatgcttc
    31321 tttaaatatg ctgcaagcat ttgttcattt gctgtctcgt aagctccttt actttcttta
    31381 gcaaatatat tttgtaaaaa atcgaatatc ttaacatata tttgctgtcc aaaagaacga
    31441 tgtttctttt tttctccttt tatctccgcc agcaaaacac ctgccaactc gtcctctgca
    31501 tacaaagcaa gaatttgagt tgctcgattc aactccatat acaaaaaata tcttccgtaa
    31561 agattcagca gaagttcatt ttccatatat ctctcaaagt tcattccttc aacagcaaaa
    31621 tagatagctt ttttgtaatc tttcttttct aagggtttaa aagttagttt catcatttcg
    31681 cctcctcaac ttcaatgatt gtgttatact tacattataa tagacacagt gtctactatc
    31741 tataatcaat catctataag tttgtcgatt aagcgacagc tttgctttga tatgtcggat
    31801 aaggagatag gtaaatgaaa aagacagata gacgcgtcaa aaaaacagaa aaagctttgg
    31861 ctgaaacact atccacatta ttagtaaata aaaagattca agcaatcact atacgcgaac
    31921 taacagaaac ggcagatgta catcgttcga ctttttatac acattataag gatatctatg
    31981 atctatatga tcaattagaa agcaactttt ttcgggatct aaatgaaatt ctctcttatg
    32041 atccagccca tagctatgag gaactataca ctcgcttgat tgactatgtt tacgcaaata
    32101 cttttttata tcgcttattt atcctcaacc atggtgatat ttcgtttcag aagaaagtta
    32161 aacaagttat tgaagaaaac tatctgaaca tatggctata tgaagacaag aaaacgatta
    32221 tcacagaaga aatgagattc tttactacgt accacgtcca aggttgtcta tccgttctca
    32281 atcggtgggt agaaagcaat tacaagtact ccaaaaaaga attattagaa ctcttacaga
    32341 aactgaatga tcacatagag aaaatcatgc cttgaataaa aacatactaa gcttagttaa
    32401 gaagaaccgt aaccttctcg tataacaact gaatcttatt tagccaaatt gtttactgaa
    32461 gattttttga gtaaaatttc tataattcct acttattgaa aagtagtttg tagtgctgct
    32521 tctccctctc taagtgatat atcttgttgt accaatcaaa ttcacctcat cattaataaa
    32581 ttacttttag gctaatttat tacaacttat aggttgaagc tgtaaccaat ttatagtcat
    32641 attatatttt tatcatccag tttattgaat tcttctcgtt tacctcaatt cattttcaag
    32701 gggaatcagc tcatcatcta acattttacg ccatgctcgc ttcatttctt gtgggtattt
    32761 aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac tgttgataat
    32821 tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag aagcatgtaa
    32881 ttttttctca gaaacaaacc actcattgga aaagacatga atcaaatcat cacttacttg
    32941 tgtaaaatag cgacgtttaa tgacaaagat atcttcattg ctatctagtg taccttcatt
    33001 tctttgtgct taaattcaaa gataattttc tcaataaaga tttttgattc cagtaagatt
    33061 cctttactcc ttttacaaca tagcgttgtc ctttgttttg aaacatcttc ttcccctctt
    33121 tgccattaaa aatagcccaa ttctctataa agaagattaa gcttcattat tataatataa
    33181 agtttaaatt tggtttatta cttacttttt cttattctct agtaagctaa tattgaaaaa
    33241 aatgtaatca gatcattatt tttatatgtt gtaacttcct gataagaaaa ctaattaaaa
    33301 gaaagaggta actacataaa ctaattaaaa aagctaactt catagttgga ggataaatgg
    33361 aagaaacttc aatgattgtt tctccttgtg ttccttcaat cgacactagt cctattatca
    33421 atctctcttt tttgtccatt tacttttgcg actgttcccc aataatcaaa tacaggaagc
    33481 gttatttttt gtcctttaga ttcatgtact gtatagacta ttttcgaatc catcacttga
    33541 taaggcatag tatgccattc cttgccaatt cttacttcat gattctgcaa atgttctttg
    33601 tattgtaatg cttccgtggg tgcatagtca taaagtcctc cgggaaggag tgtcgtgtaa
    33661 cgaataagat tttcttgatt cattaccaag tgatctctct gagtgacaac tttatgttca
    33721 tagtttgctt ttgttgcagc agatacagcc aacagtaata aaacaattgc cgttatatta
    33781 actacttttc tttgcttagc tgatgtgctc ttcatgaaaa cgaacgataa aatcaacgag
    33841 ccaaacagta cttgaaagcc taagattcgc caaggaaatt gaatcatttg aatgggtgta
    33901 ttttgtaata aagaccatgg aaaaaatttg gtggataaga gccaagagac taccgcacta
    33961 aggtaaatca ctttttcttt gcgattaaat cctttaaaaa agaagagtgg acagatcaaa
    34021 gaaataatac caatgattcc taccgtaaag gttgtcaggt cattattcat cgcaccaatc
    34081 aaactatccg agacatttaa tgcttggtgt tgtaaggtcg ttttatctgg ctcattaatc
    34141 gattgaacca tgatttgtcg gaacatcgga taccaaaagt atgctgttaa aagaatcgtc
    34201 attcctactg cctttgacaa ctccacaatc aaccggatgc tttctttttt tcttgtttca
    34261 cttactacaa agccgcctaa gactagtaag ccaacggcta ttacactcat aaaagtggaa
    34321 agaacatgtg tataaatgag taagctcata ctgactgcta atggaattgc acatggcttt
    34381 ttctcataaa tcagtgcgta agcataatac ataactatcg gtataaccgt aagtgcaatc
    34441 gcttcagcaa tagcagaacg atgataaata tcaatcgtac ggtacccata aaatgtatat
    34501 aaacagctaa atataaaagc cgcatcgatt ctttttaaaa attttagtcc ataatgaaac
    34561 atcagcatga tagtacaaac attcaatagc caaacatata agatataact aacgattaaa
    34621 ttattactta tccaataaaa aatgactgcc ggaaaaaagg tcaagtaggg ataaaaataa
    34681 ttcactccgc tcccataatg attaaacgtc gtaaaattga ttggaccaga aaaaattgaa
    34741 gataaccctt tgactctagc aaaatggaag gcaatatccc ccgatgtaga aatagtatga
    34801 acatttctaa aataattgat tgaataaagt ccagcaataa ttgctaggag aatggacaat
    34861 attatttttt tctttacttg cattacttaa ttatatactc ctttcatgga ttgctttatc
    34921 tagtataact taaattttaa ataaatcgtt atttattttt actctcactt aaaaaagcac
    34981 taaaattata agagagggag gtaatggtct gaacaccaag aattgttcca acatcgaatt
    35041 gcttaactaa tgagttcaaa gcttaattat gtggctctta tatttaccga gcgtggattg
    35101 cgatctcttt agcgaaacaa acatctttca taaaaaaaac agccatatcc gctttcttgg
    35161 atatgactat ttactactac tttactatct ttcattcttc ttgtctcact tatgataagg
    35221 cgctgcgtat catgagtgaa tgatattagg aaaacttc
//
LOCUS       DARBEG010000021        34636 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000021 DARBEG010000000
VERSION     DARBEG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.021"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            591..1280
                     /locus_tag="SCK20_001012"
     CDS             591..1280
                     /locus_tag="SCK20_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1145095.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="SCK20_001013"
     CDS             1267..2475
                     /locus_tag="SCK20_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1145096.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="SCK20_001014"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="SCK20_001014"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG1145097.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SCK20_001015"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SCK20_001015"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG1145098.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="SCK20_001016"
     CDS             complement(6578..7273)
                     /locus_tag="SCK20_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG1145099.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SCK20_001017"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SCK20_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG1145100.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="SCK20_001018"
     CDS             complement(8472..8663)
                     /locus_tag="SCK20_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG1145101.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="SCK20_001019"
     CDS             complement(8684..9574)
                     /locus_tag="SCK20_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG1145102.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="SCK20_001020"
     CDS             complement(9561..10328)
                     /locus_tag="SCK20_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1145103.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SCK20_001021"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SCK20_001021"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG1145104.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SCK20_001022"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SCK20_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG1145105.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SCK20_001023"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SCK20_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG1145106.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="SCK20_001024"
     CDS             14899..15282
                     /locus_tag="SCK20_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145107.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="SCK20_001025"
     CDS             complement(15450..16208)
                     /locus_tag="SCK20_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG1145108.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="SCK20_001026"
     CDS             complement(16226..17044)
                     /locus_tag="SCK20_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1145109.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SCK20_001027"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SCK20_001027"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG1145110.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SCK20_001028"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SCK20_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG1145111.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="SCK20_001029"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="SCK20_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG1145112.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="SCK20_001030"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="SCK20_001030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG1145113.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="SCK20_001031"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="SCK20_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG1145114.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="SCK20_001032"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="SCK20_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG1145115.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="SCK20_001033"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="SCK20_001033"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG1145116.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="SCK20_001034"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="SCK20_001034"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG1145117.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="SCK20_001035"
     CDS             27086..27385
                     /locus_tag="SCK20_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG1145118.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="SCK20_001036"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="SCK20_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1145119.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="SCK20_001037"
     CDS             27973..28209
                     /locus_tag="SCK20_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG1145120.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="SCK20_001038"
     CDS             28352..30325
                     /locus_tag="SCK20_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG1145121.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="SCK20_001039"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="SCK20_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG1145122.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="SCK20_001040"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="SCK20_001040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG1145123.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="SCK20_001041"
     CDS             33204..34496
                     /locus_tag="SCK20_001041"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG1145124.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DARBEG010000022        34330 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000022 DARBEG010000000
VERSION     DARBEG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.022"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SCK20_001042"
     CDS             202..1077
                     /locus_tag="SCK20_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145125.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SCK20_001043"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SCK20_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="SCK20_001044"
     CDS             complement(1642..2973)
                     /locus_tag="SCK20_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1145126.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="SCK20_001045"
     CDS             complement(3122..3835)
                     /locus_tag="SCK20_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1145127.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="SCK20_001046"
     CDS             4010..5434
                     /locus_tag="SCK20_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1145128.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="SCK20_001047"
     CDS             5733..6440
                     /locus_tag="SCK20_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1145129.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="SCK20_001048"
     CDS             6427..6750
                     /locus_tag="SCK20_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1145130.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="SCK20_001049"
     CDS             complement(6839..7876)
                     /locus_tag="SCK20_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1145131.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="SCK20_001050"
     CDS             complement(8197..9180)
                     /locus_tag="SCK20_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG1145132.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="SCK20_001051"
     CDS             complement(9595..10365)
                     /locus_tag="SCK20_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145133.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="SCK20_001052"
     CDS             complement(10358..11293)
                     /locus_tag="SCK20_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145134.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="SCK20_001053"
     CDS             complement(11609..13258)
                     /locus_tag="SCK20_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1145135.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="SCK20_001054"
     CDS             complement(13375..15978)
                     /locus_tag="SCK20_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1145136.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="SCK20_001055"
     CDS             complement(15990..17039)
                     /locus_tag="SCK20_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG1145137.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="SCK20_001056"
     CDS             complement(17065..17379)
                     /locus_tag="SCK20_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG1145138.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="SCK20_001057"
     CDS             complement(17395..17841)
                     /locus_tag="SCK20_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1145139.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="SCK20_001058"
     CDS             complement(17842..19764)
                     /locus_tag="SCK20_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEG1145140.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="SCK20_001059"
     CDS             complement(19976..20872)
                     /locus_tag="SCK20_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1145141.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="SCK20_001060"
     CDS             complement(20865..21545)
                     /locus_tag="SCK20_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1145142.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="SCK20_001061"
     CDS             complement(21546..22328)
                     /locus_tag="SCK20_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145143.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="SCK20_001062"
     CDS             complement(22321..23238)
                     /locus_tag="SCK20_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1145144.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="SCK20_001063"
     CDS             complement(23402..23725)
                     /locus_tag="SCK20_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145145.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="SCK20_001064"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="SCK20_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG1145146.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="SCK20_001065"
     CDS             27853..28167
                     /locus_tag="SCK20_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1145147.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="SCK20_001066"
     CDS             28180..28554
                     /locus_tag="SCK20_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1145148.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="SCK20_001067"
     CDS             28564..28899
                     /locus_tag="SCK20_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG1145149.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="SCK20_001068"
     CDS             28982..30322
                     /locus_tag="SCK20_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1145150.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="SCK20_001069"
     CDS             30400..31074
                     /locus_tag="SCK20_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1145151.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31067..31231
                     /locus_tag="SCK20_001070"
     CDS             31067..31231
                     /locus_tag="SCK20_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145152.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            31307..32449
                     /gene="dcm"
                     /locus_tag="SCK20_001071"
                     /pseudo
     CDS             31307..32449
                     /gene="dcm"
                     /locus_tag="SCK20_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            32439..32729
                     /locus_tag="SCK20_001072"
     CDS             32439..32729
                     /locus_tag="SCK20_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145153.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="SCK20_001073"
     CDS             32988..34172
                     /locus_tag="SCK20_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1145154.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34169..34306
                     /locus_tag="SCK20_001074"
     CDS             34169..34306
                     /locus_tag="SCK20_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1145155.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DARBEG010000023        33520 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000023 DARBEG010000000
VERSION     DARBEG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.023"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(201..635)
                     /locus_tag="SCK20_001075"
     CDS             complement(201..635)
                     /locus_tag="SCK20_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145156.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            783..1924
                     /locus_tag="SCK20_001076"
                     /pseudo
     CDS             783..1924
                     /locus_tag="SCK20_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2050..3129)
                     /locus_tag="SCK20_001077"
     CDS             complement(2050..3129)
                     /locus_tag="SCK20_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1145157.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3232..3672)
                     /locus_tag="SCK20_001078"
     CDS             complement(3232..3672)
                     /locus_tag="SCK20_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1145158.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3729..4139)
                     /locus_tag="SCK20_001079"
     CDS             complement(3729..4139)
                     /locus_tag="SCK20_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1145159.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4319..4753)
                     /locus_tag="SCK20_001080"
     CDS             complement(4319..4753)
                     /locus_tag="SCK20_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1145160.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4853..5701)
                     /locus_tag="SCK20_001081"
     CDS             complement(4853..5701)
                     /locus_tag="SCK20_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1145161.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6090..7856
                     /locus_tag="SCK20_001082"
     CDS             6090..7856
                     /locus_tag="SCK20_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG1145162.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7958..9217
                     /locus_tag="SCK20_001083"
     CDS             7958..9217
                     /locus_tag="SCK20_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1145163.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9364..10665
                     /locus_tag="SCK20_001084"
     CDS             9364..10665
                     /locus_tag="SCK20_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1145164.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10668..11519
                     /locus_tag="SCK20_001085"
     CDS             10668..11519
                     /locus_tag="SCK20_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1145165.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11532..12347
                     /locus_tag="SCK20_001086"
     CDS             11532..12347
                     /locus_tag="SCK20_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG1145166.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12498..13664
                     /locus_tag="SCK20_001087"
     CDS             12498..13664
                     /locus_tag="SCK20_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1145167.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13894..14541
                     /locus_tag="SCK20_001088"
     CDS             13894..14541
                     /locus_tag="SCK20_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1145168.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14625..15569
                     /locus_tag="SCK20_001089"
     CDS             14625..15569
                     /locus_tag="SCK20_001089"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG1145169.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15704..17155
                     /locus_tag="SCK20_001090"
     CDS             15704..17155
                     /locus_tag="SCK20_001090"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG1145170.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17176..18012
                     /locus_tag="SCK20_001091"
     CDS             17176..18012
                     /locus_tag="SCK20_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1145171.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18012..18671
                     /locus_tag="SCK20_001092"
     CDS             18012..18671
                     /locus_tag="SCK20_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1145172.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            18797..19486
                     /locus_tag="SCK20_001093"
     CDS             18797..19486
                     /locus_tag="SCK20_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145173.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19492..20448
                     /locus_tag="SCK20_001094"
     CDS             19492..20448
                     /locus_tag="SCK20_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG1145174.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20492..21004
                     /locus_tag="SCK20_001095"
     CDS             20492..21004
                     /locus_tag="SCK20_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145175.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21260..21808)
                     /locus_tag="SCK20_001096"
     CDS             complement(21260..21808)
                     /locus_tag="SCK20_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1145176.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21897..23078)
                     /locus_tag="SCK20_001097"
     CDS             complement(21897..23078)
                     /locus_tag="SCK20_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEG1145177.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(23314..24822)
                     /locus_tag="SCK20_001098"
     CDS             complement(23314..24822)
                     /locus_tag="SCK20_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG1145178.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24981..25172
                     /locus_tag="SCK20_001099"
     CDS             24981..25172
                     /locus_tag="SCK20_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145179.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25375..26292
                     /locus_tag="SCK20_001100"
     CDS             25375..26292
                     /locus_tag="SCK20_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG1145180.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26285..27358
                     /locus_tag="SCK20_001101"
     CDS             26285..27358
                     /locus_tag="SCK20_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145181.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27506..28024
                     /locus_tag="SCK20_001102"
     CDS             27506..28024
                     /locus_tag="SCK20_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145182.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            28005..28397
                     /locus_tag="SCK20_001103"
     CDS             28005..28397
                     /locus_tag="SCK20_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG1145183.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28473..29408
                     /locus_tag="SCK20_001104"
     CDS             28473..29408
                     /locus_tag="SCK20_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG1145184.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29541..30320
                     /locus_tag="SCK20_001105"
     CDS             29541..30320
                     /locus_tag="SCK20_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1145185.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30313..30630
                     /locus_tag="SCK20_001106"
     CDS             30313..30630
                     /locus_tag="SCK20_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1145186.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30874..32484
                     /locus_tag="SCK20_001107"
     CDS             30874..32484
                     /locus_tag="SCK20_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1145187.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32641..33489
                     /locus_tag="SCK20_001108"
     CDS             32641..33489
                     /locus_tag="SCK20_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1145188.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        11088 a         5982 c         6510 g         9940 t
ORIGIN      
        1 ctatttagtc ggtcaagtac ttctttttta aaaaaagtat tcatcataaa agccactact
       61 tgcggatctt catcataaaa ttctgtagca gtacttgaaa agatgttcca ataaatatct
      121 aatgctgcat attgaatttg tacctctttt ccagaaaaag actttctgcc attattagaa
      181 aaggagatag acaaaccgta ctatgctaaa aatgcattaa tctttttcat tttcgtatac
      241 aaataagagg cactatagtt tgtttgctga ctgaaactga taatcgaatt tgtctgacag
      301 ctgaacaaag aagtcaagat ctgataactg actgattcgt tgcagtatct tccaaataga
      361 cgataatagc agtaagccgg atcaattcct tcaaaattca actttaaaaa aggtttttgc
      421 ttatcaatat gcagttctgt tagattgaga cgctcaaagt cttgttctaa ttctttgatg
      481 tatagtaaaa aatttgattc agacatatgg aaatgatctg ctaaatattg tgtagtaata
      541 aagaaacgat gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc
      601 aagataaacg ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat
      661 tgttaaaact tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca
      721 tataggtaga atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag
      781 ttatgagata ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta
      841 taaatcagac agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg
      901 ccaacgtcaa tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa
      961 aattaagaaa aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag
     1021 cacctatact acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct
     1081 atgacgagaa cactttgact atccaacaca agcaagcgat atttaccttc accgttacat
     1141 taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga
     1201 acgatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg
     1261 actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag
     1321 aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag
     1381 aacccgatac agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg
     1441 gagaaaaacc agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg
     1501 aaacaaatcc agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag
     1561 aagaaaaacc agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag
     1621 aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc
     1681 tagcaattct aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg
     1741 cccaatcagc tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa
     1801 aaataaacag ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat
     1861 cattctatta agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct
     1921 ctaacatttg aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat
     1981 gaccccaaaa taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc
     2041 atatttattt taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt
     2101 caatacatct aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa
     2161 gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct
     2221 ggacatcaac ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat
     2281 caggatataa acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat
     2341 acaagaaatg ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa
     2401 actcagcagc tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa
     2461 aagagcgaca acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc
     2521 aaaatagtaa atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa
     2581 tacaattgtt tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa
     2641 cgccactgcc atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg
     2701 aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc
     2761 tgcatctttt ggctgatgct cgtaaaaatt aataacggac tggatcatgt caaatgtttg
     2821 atgaataagc atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt
     2881 ataaatcatg atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac
     2941 aagatgaatc atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct
     3001 aactaagaaa agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt
     3061 cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac
     3121 gttattcatt atatgatgga atatgaaaat aagaaagagc aaaaagaaaa accgctaaat
     3181 gacgttatct tatgagttgg ttttcctcat aaaatcgttc atctagcagt ttcagccctt
     3241 tttctggtct tgatgaaaat tatctagcat ttttcgggtc agatgttgca gtgtatccaa
     3301 ttcttcctgt tcaagtggaa gataagactt gatcttttgt ggaatacctt ctgcttgttt
     3361 ttgcaacgca gcaccttgag cagtcagcgt aacatttacg attctttcat cacccgtttc
     3421 acgctgacga ttaatataat ttttttctgc taactttttt agtaagggag tcagcgtacc
     3481 agaatccaga cacaaatcat ttcctagctc tttcactgaa gtctttttct tttcccataa
     3541 aactaacatc acaagatact gggtataggt aagattcagc ggcttgagga ggggagtgta
     3601 gtgtctaatg atttctttag aggcggcata taaaggaaaa catacttgat taactaaata
     3661 tagatcttcc atttttccac ctccgctgtt ttactatagt gtatcatagc ggatttacac
     3721 aaaaaagtct attcactaac tgctttcaga gtaacttcta tatttccagc tgtagctttt
     3781 gaatacggac acactttatg cgctgcctct aataattctt gcgtttcttc cagagaaaga
     3841 ccttcgatag atccttcaat ctctacacct aagacaaagc tcgtttcact ttctgaataa
     3901 agggaaactt gtgcactgac agtagaagcc ccagagatat ttttttgctg tttgatcaat
     3961 tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa acaattgttc agggttggtt
     4021 gcattttcga cgcgtttacc aggagctgca atattcaatt cgaatgtacg atcgggatta
     4081 tgcacttctc cagagcgtcc gcctgtgttg atcattttcg tagaatacat ttttttcata
     4141 aaattaccac ctttaattat attttataca attgaatttt acacaatata attgtggaaa
     4201 gcaaagaaat ggattggcat aagataaata agagaaagca catgaaacag acgcgctttc
     4261 tcgaatagca aacatataca aaaaatatca gagacaaaaa ataatctcat cataaacatt
     4321 acatatattt ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga tggacctgat
     4381 gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca ccaaagtcca
     4441 tcatttctgt catttcttga atctctacac agccatttcg taatgcttta ttatgtgtgt
     4501 cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca gcagtaggag
     4561 cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtacca tacaaggtaa
     4621 aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt atttcatcaa
     4681 agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa tcgatttccg
     4741 ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag attcttttat
     4801 tataccagct aaagaaaacg cattcaaaaa aatagcggat ttacttacca actcattcga
     4861 ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca atgacaaagg
     4921 cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga tcagtcacaa
     4981 cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct agcagggtaa
     5041 ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa tgttcactca
     5101 tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg acaattgaag
     5161 tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaata aacagattaa
     5221 gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag tggaaatatt
     5281 ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt ttatataaaa
     5341 tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac cgatctgaag
     5401 taatcattgt ttctggaact attgcaatga ttaaaggaaa aaccaagctt ccgaaaagta
     5461 ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata acgatattca
     5521 acactaaaat aactatagct cggtcaaagc ctaaagcagc ttccagtaat attcctagac
     5581 cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata gcgactccta
     5641 atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc gtttctttca
     5701 tttattcact tccttaattc attaataata ctcttatctt attataactt acttttcacc
     5761 ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg tttcattctt
     5821 ctagaatata cttctacgcc aaaaactgtt aaaaaacttt tactttagaa aattggagaa
     5881 attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagac taaaactttt
     5941 aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt atcaaaaaac
     6001 tgttaccgat aacatttttg aaagcgttgt cttttgcttc tctatcactt atagtgatga
     6061 cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat catcgaccgg
     6121 aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt cgtactagga
     6181 aaaatgatat caaaaaagta tatgtgctat gtggagatcc atataccata acaactgaaa
     6241 aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt catgattatt
     6301 gggaaattga agtgacgagc gaaacaagtc gcttacagta tgcgttccat gttgttgggg
     6361 aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga gaaaatgtga
     6421 ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat cgattcacta
     6481 ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga tttgcaaacg
     6541 gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat ccagaccgag
     6601 aagacttttt cgggggagat ttgcaaggtg tgatggatca tttggattac ttgacggacc
     6661 ttggcatcaa tggcatctat ttttgtccca tatttaaagc cacatccaat cataaatacg
     6721 atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt ttaaaaaact
     6781 tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta ttcaatcata
     6841 tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa tcgatctata
     6901 aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact gacttaccag
     6961 aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg ccgaaactaa
     7021 atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat tggataaaag
     7081 aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat catttttgga
     7141 aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt ggagagattt
     7201 ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg aattatgcat
     7261 ttacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca caaatggtca
     7321 gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga acattcaact
     7381 tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac aaagaattga
     7441 tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc tattacggga
     7501 cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg atatgggaga
     7561 aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct ctaaggaaac
     7621 agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtaaat gatcgtgaag
     7681 ataagctcta tttcacacgg aaactagagg gacagatcat ctatgtttac tttaatcagt
     7741 caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa aattgtcagt
     7801 tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg tgttgaaaac
     7861 tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat tataataagg
     7921 gtgtaacagc acgttgaata caaaggagga aatgaagatg aaaaaaagaa gtgtgaaaca
     7981 gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt gtggcaatgg
     8041 taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt ccgttgattc
     8101 tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa acgatgtgaa
     8161 aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt tagatgggcc
     8221 tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg cgttaggaca
     8281 gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg aaacagacaa
     8341 agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg agacccttgt
     8401 tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag atttggagaa
     8461 tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata caggattttt
     8521 agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg gtggctatgt
     8581 atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag gtgctgtaga
     8641 aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa tgcaagacat
     8701 aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg cagcaatcat
     8761 tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg gtgtagccaa
     8821 aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa aaggatgggt
     8881 agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt atgtaacgaa
     8941 tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa accaacaagc
     9001 tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg aacaatatga
     9061 aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag gtgcagaaaa
     9121 cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag atgaagcagt
     9181 aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac cgtccatttt
     9241 tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg aaaattatct
     9301 tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag agggggcatt
     9361 cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct ttgttcactt
     9421 attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt ttttttcgcc
     9481 atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt ggtaggattg
     9541 gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat tgaaggcact
     9601 ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct tttagacgct
     9661 tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat gaaggaagtc
     9721 atacataata taggtgatga aggttttcct tatctattga cacttcccgc ttatcttctc
     9781 atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt tactaattat
     9841 gacttcaatc atattccacc agccagttta attgattggg taggggctaa aaactttttt
     9901 agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc gtggaccgtg
     9961 atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac agcggttgtt
    10021 gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt attaccatgg
    10081 gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga tagcgtaggt
    10141 gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga tatcagtgca
    10201 atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt gcagggctgg
    10261 ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat ttcagaagac
    10321 ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc aaacatcacg
    10381 cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac ggggaatttc
    10441 aataatttct ccatgatcta tctgtttaac aatggcggtc ccggaagcgt aggcggagga
    10501 gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg aacttctccg
    10561 cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat ctgtgtgtca
    10621 ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg aaaaaatttc
    10681 attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt tttcttttaa
    10741 tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc ttcaagtcag
    10801 gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga aactttactc
    10861 ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc atttctgtcc
    10921 taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat agtcgctatc
    10981 gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg gtaccgacta
    11041 tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc gataagtact
    11101 ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg ctgatgaaag
    11161 gatactttga cactgtacca atagatttag atgaatctgc taaacttgat ggggcagggc
    11221 atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc gccgttcaag
    11281 cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc ctgcttcgaa
    11341 cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat gtgacaaacc
    11401 aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt tgtctattat
    11461 tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca aaaggatagg
    11521 agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt acgccaactc
    11581 aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata tttgtatttt
    11641 tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc caagcgattc
    11701 cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc caaaaatttt
    11761 ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata gctgtgaccc
    11821 ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat ggaactttta
    11881 ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt atacgccgct
    11941 atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc gaagatttcc
    12001 aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt ttatcttata
    12061 gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc ggggcttcct
    12121 ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc aaagagagtg
    12181 caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt gtagggttta
    12241 tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc ttgctgcttt
    12301 tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa gtttaagcga
    12361 tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa aaatttctga
    12421 aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat agggtagttt
    12481 ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt tcacgttctt
    12541 tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac ggagatacgg
    12601 acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa gagattacaa
    12661 gtaacgcatc tttggcacta ggagaagcct atatggatca tcgaatcgag atcgagggaa
    12721 gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta cgaggaaaag
    12781 attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac aaagaagata
    12841 tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat ccaactttga
    12901 cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca caaatcaata
    12961 aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg cttgatatcg
    13021 gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc aaggcaactg
    13081 ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa gaagaacatt
    13141 tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc gaaacgtttg
    13201 atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt gaaggttatt
    13261 tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat ggaatcagcc
    13321 gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct ggcggttatg
    13381 tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa ctgattgatt
    13441 tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag aattttcatc
    13501 aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg tgggatctat
    13561 atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt caatatttgc
    13621 ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg caaaagtact
    13681 gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg aacttttttg
    13741 tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt tcataataaa
    13801 agtttggttc tttaattttt aaacatgaca gaaaattttt tcttcgttat acttgtaata
    13861 gaatactatt ttataacaaa ggggatcgaa tcaatgaaaa aaactgttat ttatactagt
    13921 ttatttgcag ctttgttttt agctgcttgt caatcaaata ccaatcaagg cacatctggg
    13981 aatacgtctt ccagtagcga acagcgctct tcacaggtat cggactcctc aacaaaagca
    14041 agcacatctt cgtctagcca gacaaaaagt tctagtgaag caagtactca gtcagatatc
    14101 aaagtgtctg cagctcgtgc gatcgaattg ttccaagata aatacccaga tactgcaatt
    14161 actagtttgg aattagatag tgattgggga tcttacttct acaagataga aggtgtcgat
    14221 gatcaaaaag aatacgaagc aagaattgat gctcaaaatg aaaaaatgga gacagagaat
    14281 ccagaaactc tagatcgaga tgaacaaaat ggtcaaaaga aaaaagaaga tggtctggat
    14341 gttgataaat tgatcacgat tgaggaagca gcaaaaatcg ccgtcgacaa agtaggaagc
    14401 ggtacggcaa cagattggga tttggataaa gatttaggta ctacttattg ggaagtcaaa
    14461 gtaaaagacg gcaatcaaag tacatcagtc aagatcaata gccaaacagg cgaagtactg
    14521 tcaactgaac aagatgatta agaatttctt cggaaaacaa gagttgattt ttcttttagt
    14581 catgatacac tagacaaaat aacttgtttg aaggagcgat ttacttgagt ttagaaacga
    14641 cctttgccaa tcatatcttt gccaatcctt tgatgaatgc atctggtgtc cactgcatga
    14701 ctacccagga attagatgaa ctggctcatt cagaagcagg agcatttatc actaagagct
    14761 gtacgatcaa tgaacgaaaa ggaaatcccg aaccacgcta ttttgatgtt cctttaggga
    14821 gtatcaattc tatgggactt ccaaacttag gtttttctta ttatctagag tatgctcttg
    14881 cgtatgaaaa agtgcaggaa aaccaaaatc aaccgttatt tttttccatt gccgggatga
    14941 gtgtccagga aaacttggaa atgcttgaga aaatagaaaa aagtgggttt aatgggatca
    15001 ctgaattaaa tttatcttgt cccaatgtgc ctggaaaacc acaactagct tacgattttg
    15061 aggccactta tgaaacactg aaagaagtat tcagcatctt ttctaagccg ctaggaatca
    15121 aattgcctcc ttattttgat tttgctcatt ttgatcagat ggctgatatc ttgaatcagt
    15181 ttccattgac ctatgtcaat gctatcaaca gtgtgggcaa tggtttgtat atcgatacag
    15241 aacaagaagc agtagtcatc aaaccaaaag aagggttcgg cggcattggc ggagagtata
    15301 tcaagccgac tgctttggcg aatgttcgcg ctttttatac acgtttgaaa ccagaaatcc
    15361 aaatcattgg tacaggtggg attcggacag gacaagatgc atttgaacat ctgctatgtg
    15421 gcgcaagcat gttacagatt ggtaccgagc ttcataaaga agggccggaa attttttcta
    15481 gaatcataaa agaattaacg cagattatgt cagaaaaagg atatacgtca attgacgaat
    15541 ttaaaggcaa attacgcacc atttcatagc aaagagacga aggtcatgat tgatcttcgt
    15601 ctttttttat aaaaaacact cgtctgttca ttcctgtgca agcgtactca ttgtatgtta
    15661 gacgaacttg tgggaaaata acagaggcaa aaaggaggat aatatgaatg atgcaacaga
    15721 attagcaaaa gagcttaaag aaaaagaatt tagttttcaa gaactagtta cggatataca
    15781 gaaaaaaata aagaagcaaa atcatgaatt gaatgcgttt gtcgcgcttg aaccattcga
    15841 gagtaattcc cacgagctat tgattcccca gtcagaaaaa gggccattgg ctggtatccc
    15901 atttccttta aaaatgctcg gtcaagaaaa aaagggatgg ctagctactt caggatctcg
    15961 tttatttgaa acacaccgag cttctcatac atctaattat gtgcggcaag cagaagcgat
    16021 aggtctagtt ccttttggac aaaccaatgc gccagagttt ggtttcaaaa atattacaga
    16081 cccagtcatc tatggcccag caagaaaccc ttggaacctt gaatgtacac cgggcggatc
    16141 aagtggagga gctgctgctg cagtttcatc aggaatcgtt cctttagctg gtgctagtga
    16201 tggcggtggt tctattagaa tacccgcttc gttttgcgga ttgattgggc tgaaaccttc
    16261 gcgagggacg atgccagttg gtccatatgc ttggagagga tggcaaggag ctgctattga
    16321 tttcggcttg acagtttcta tgagagacac ggaggcttta ttttatggga tgagaagtat
    16381 ccattccggt gcgccatatc aagtatcgcc agttgagtgg caaacacatc caagaaaaaa
    16441 acggttgaag atagcttttt gtgttgattc accgatccac tcacagatta gtgaagaggc
    16501 agtacttgct gtaaaaaaag ctgtagattt ccttggaaaa gaagggcatg atttgacaga
    16561 gattccttac ccactagatg gtcgtaaact gatcacgtct tattatcaga tgaatggcgc
    16621 agaaacagca gcaatgatcc gctcgattga aacaggcatc cagcgagctg tcagaaaaga
    16681 agaacttgaa cccatttctt ggactttgtt ccaatatggt cagaagctct ctgccgctac
    16741 ttacatcgat tctctgcatg tatgggatga agcagcaatt gtcatggaaa acctacatca
    16801 agaatacgat ctattccttt caccaacgac tgcatttacc gcccctaaaa tcagcgaaga
    16861 tttgcaaagt gatgcgataa gagcacagat ggaacacatc agtgattgtt ctgaagtaga
    16921 aggtttagac gttgtttctt ccatgtttga ggagagcttg aagatcacac cttatacaca
    16981 gttggctaat ctgactggac aaccagcaat cagcttgcct actcatttga caaaagaaaa
    17041 catgcctcta ggaatccaat ttatggctgc cagaggaaga gaagatttgc tatttcaaat
    17101 cggctatcta tttgaagcaa atcatcagtt ccatttacca aatccatgga aatagcagaa
    17161 acaaggagaa gaagaatgaa gaaaaaaaga ttgtttgctc taataactag tgcctttttt
    17221 attttaagcg cttgcggtac agctgccgct aatcaggaag ataacagctg gagtaatgta
    17281 gaaaaaaaga aagaattagt agtagcgact tctggcacac tgtttcccgc atcttattat
    17341 aatgatcaaa atgaattagt tggttatgat gtagatttgg caaaagaggt ggctaaacgg
    17401 ttaaatgtca aaatctcctt taaagaatac aacgtggatg gacaaatcac ttctgtttca
    17461 aaaagagaat cagattttgc tgcgaacgat tttggtatca caaaagaaag agaaaaaaaa
    17521 ttctcccttt cagagacttt caaatattca tttgacagta tgatcgtacg aaaaaaagat
    17581 gattcaggaa tccactcact tgaagatctt aaagggaaaa aagcagctgg cgaacccaat
    17641 actagctata tgcgtttagc ggaaaaacac ggagctaaat tagtcacata tgacaatgcc
    17701 acgaatgatc agtatttaag cgatgtagca aatggtcgaa cagatgttat tctaaatgat
    17761 tattatctgc agaaaatggc cgtatctgct ttaccagata tccctgtaaa aatactggag
    17821 aacgtttatt tcaatgaaaa cgaaagtggt tttttattga aagaaggaaa tgatgctctg
    17881 aaaaagaaaa tcgatggcgt attaaaagag atgaaagaag atgggacgac aaagaaaata
    17941 tctgaaaaat attttcaaac tgatgtttca gtcaaaccga aagaaacgat ggctgagtcg
    18001 gtgagtgagt gatgtacttt cccctaattg atttttccct catgtggact tcacttcctt
    18061 tcgtactgca aggcttggta tacacattag ggattggctt cgtgtcgttt cttttaggaa
    18121 atttggtagg gttgcttttg acagttttag gtcttttgga ttggctgcct ctgaatgtat
    18181 ttattcgttt ttatttatcg ttctttagag gcattcccgc actagtcctg ctgtttctgt
    18241 tatatttcgg gttaccttat caattaagtg ccttaacggc ttcagtcatc tgctttacga
    18301 ttaccagcag tgcatttatt ggtgaaatct atcgaggatc actagcaggc gtttcttcag
    18361 gtcagtggga cgctggttta gcttctggtt tttctttctt tggggtattg aaatatatcg
    18421 ttttgccgca agcttttcga atctcagtac cggcactcag taacgtagct atggatctag
    18481 tgaaaggtac ttcgttagca gcaacaatca cagtgccaga aatatttcaa aaagcaaaga
    18541 tcataggcgg tcggacattc gattattttt ctctctatat tttagttgca ctgatttatt
    18601 gggtcatttg tatcgggatc gaaaaagtgc aacagtattt agaaaaacgt tctatccatc
    18661 agtcaatctg aaaaatagaa atggtgctag cgtggctggc accatttcta ttttttgttt
    18721 cttgtatatt ctgcctagta ttttaaaacc atttcatgta aaatgtaaat cgaaaatatg
    18781 ttcggaggta gaaaacatga agaccccgct aaccatcgaa atagaatcct cgttatacca
    18841 atattgcatc gagcaaggcg gcttggttgt agaagaagtc acgatgcctg atgaccaagg
    18901 aattgtagat acgcttagct gcttgacaaa atttgatgga agccgagaat ggcgatgcta
    18961 tgagctgaaa gtgacaaaag cagattttcg ctctacagcc aaactgtcat ttgttggaca
    19021 ttataattat tttgttttac caagtaaact atatgaagaa gtaaaaactg aaatccctac
    19081 aggaataggt gttttagtgt atcgtccgta cacggttgaa gaggagatgc ctgcctctgg
    19141 tacatttctg attgcgaaaa aagcagcaaa gaaagagctt gctgtaccgg aagaagcttt
    19201 gacaaaccgc ttcatggctt ctttattcag ggaagtaaga aaagcaaagc aaatggagcg
    19261 aggcgtaaaa tattttccaa aagatcaatt gtacaaagaa ttgaaaagac gttcagaaaa
    19321 tcaagatcca ttttctaacg aacgatatta tgaacggttt ttagaggaaa tagctagtca
    19381 ggcaatcaat gagttgcaag aagaagttat cgcgcttcag catgattatg aatatttgag
    19441 gcaacaacag cagatccgtc ggttgcctac agagccattg gagtgaaaaa tatgtactat
    19501 ccttatttaa gaggaaaaca atttgaccta ttagcattaa aagaagcact gtcgcgagga
    19561 ttgctctcca ataaaatcca accagtgatc gaaccagtac gtgattccgc tacgcttaaa
    19621 aatgtgatcg aattatttca aaaaaagcac catccaatcg ctgtgatcca aaatccccaa
    19681 gtaggacaat tcaaattatt cgatcagcat gtgcacagtt ggacagtcaa agaaaatagc
    19741 tctgtcgtcc ctgcacagat cttaacgcca gaaaatctca gtgaagtact ggattcacct
    19801 ccagcgtttc ttgttttcga tggacagcac tacccaaaag atacagaggt gtggaaacag
    19861 ttggcaggag ttgattcgaa atttttgata ccagatacta gtcgctttcg catctggctg
    19921 cccgaaaaca aaattgtgat ccgtgactct tttcaaacga gaaagcatgt tgaaagttat
    19981 gcagataaaa cagatgactt ttttagtgat gactatcttt ttttccacgc agacggatat
    20041 attggatttt ctgatttcac gatcgaaggt agccgttatt tcgataaagg gttccctagc
    20101 cgagcgcttg ctatccatct aacgtatatt gatgcttacg gcaatatacg cgtgaaacat
    20161 tttgtctctg acagtaacga ctctgctaaa gaccaagcat tgaagtttct tgaagcagga
    20221 gataaaatga aaaaatggat catgcgtcac catcatcaac tcttgattac ttcaggaatg
    20281 attgaattat tggctcttta ccagcaacaa aaattccctg gactgggagt gttgaaaaag
    20341 tggtcactta tgcatcattt ggaacttatt agtcacctgc tagatcatcc aaaagattgg
    20401 cttaaatatt attcaaaagt tcaggaacta tatgaagtga tccaatgatt agataatgta
    20461 aatgaaaaag gaatagaaaa gaggaaactg gatgtatatt cggcaaacaa ggaaagaaga
    20521 agtagaagaa gtggtagaaa tcattaatgc cgcagcggat ttcattgctt cgcaaggctc
    20581 tcctcaatgg caaaacgggc agaaaccaac aaaagaaacg attttgaacg atattgaaag
    20641 aaaagaaagc tatgtattaa tagtggaaga tcaaattgct ggaacagctg cattagtctc
    20701 aggcgtggat cccgtttaca caaaaataga tggtagttgg aaagaatcaa ctggtcctta
    20761 tttatcgatt catcgtgtcg ctttagcaga aagtactcga ggaaaaaaac tagcaaaacc
    20821 gttcttagaa catttgttga cgattgcagc cagcaaaaat atccaagata tccgtatcga
    20881 tacttatcca gctaatgaac cgatggaaaa gaccatctat tctgctggat tcacttatca
    20941 agggatgatt gaatttcctt ttgaacacgg cgaaagaaaa gcatatcaaa taatcatcaa
    21001 ttgactataa aatcatctga gcaaagataa agacattaac actactgaaa aaaacaatca
    21061 tatgatagcc aagaggttgt aacagaagtg gccagcttca aaaaacaaga aagaatttcc
    21121 gaaaatgctt ctcaaatttt tgtggaattt tagcgtattt tacgaaatag ctgctttctg
    21181 aaactccctt catcgagagg tcgtgacata gcttttgtca cgatctctcg gcttatccta
    21241 cgagtattat tcgatacatt tatatcaata atcctttttt tattttatct gcagcttctt
    21301 ggaacgctcc atcgtttgac acgactaaaa gtgaggggat agctttcaaa cgtttcaact
    21361 gtgctaaatc ttctacataa accgccagtc tggctgaaat ctgttcagat gaatctccac
    21421 gttttttcag gcgcgattgc aatgtttctt tagagatcgt aataaagata ggacggacta
    21481 ccgtcccgaa atactcatat aaatgccaaa aaccatttgc tgtcaaaacg gcgtaacaat
    21541 ttcctactgt cagtttcttt ttaatttctg ctttactgga accatattta tgttgagcat
    21601 attggtccca ctctgcaaac tcatttccct tgatcatctg attgaatgta ttgtcagaaa
    21661 caaaataata atcctcttga tctttttccc caggtcttgg tgatcgattc gtgtaagtaa
    21721 tgatcttttg ttccggctga aaaatagctt ctgcgagtga agtttttcca gaaccagaag
    21781 gaccgataaa tacatagcaa taattcattg tcatttctcc tttgatgatt gaacaagtga
    21841 aaaagctttg gaaaatattt ccaaagcttt taaaagtaac tttgcttttt tgtattttat
    21901 accagcaagc cagtcacaat agcacttaaa aagctgacta aagtcgctgt tagcaaaatt
    21961 ttcaatccgt gttttgcgac aagatttcct ttttcttcat ttaatccctt catcgcacca
    22021 gaaatgatcc cgatagaaga gaagttggca aatgaaacga ggaagacaga tataattgct
    22081 gttgtacgct cagtaaaatg gaaattacca tttgctaatt ctgtcatagc aacaaattca
    22141 tttgtaacca gtttagtcgc catgatacta ccggcatcca ctgcttcttt ccatgggatt
    22201 ccgctgatga aggcaagagg ggcaaagatg taacctagga tctcttggaa agtgatgccg
    22261 aatatcccgt ggaagatagc attgatcatg gcaatcaaag caacaaaacc gataagcata
    22321 gcagcaaccg tgatcgccac atggaaacca tcaagaatgt attctcccaa catttggaaa
    22381 aatgtctgtt ctttgttttc ttcaatgatc agctcatctt ctttttcttc taatacatag
    22441 ggatttacaa ttgaagccag aatataaccg ccaaataagt tcaaaacaag ggcagtgatc
    22501 acatatttag aatcgatcag tgccatataa gatccgacga tcgacataga aactgtagac
    22561 atagccgata cactcatggt gaacaaacgt ttctcagata ggaagggtaa ttcctttttt
    22621 atggaaataa aaacttccga ttgtcctaaa atagcagaag ctactccatt ataagattcc
    22681 aatttgccca tcccgttgat tttacttaat cctagaccaa cagctttcat aaataatgga
    22741 agaatcttga tatagcgtag aataccgata atagcagaaa taaatacaat tggcatcaat
    22801 acggaaagaa agaaaggtgc acttccttta tttgctacac cggcaaaaac aaagtcaaca
    22861 ccttgcccag caaaagcaag aagatgatca aacatttttg ccaatacaga aacaaccgct
    22921 gtaccaaaag ttgtacgtaa taatacgaaa ccaagaacaa gctgtaaaac gaacatcaca
    22981 attagtggtt tcaatttgat tgcttttttg ttactgctga tcaaaaaagc aagccctaat
    23041 gtgacaaata aaccaataat acctaataaa taactcaaat aatcactcct attccaaatt
    23101 ccgttttatt ttacctgttt tgcaaataaa atactaatct ttagtgaaat tttcattact
    23161 tttttagagt gaaacgcatt cattttccat tagaaaataa agcgaacaaa ataaaaggtt
    23221 taaaaagtgt aacgtcttag taaagtctat aaaaaaacga aactatcgat taagctaata
    23281 gtttcgtttt caacatttat aagtaaactt tttctattgg taatatagat tttcggaagg
    23341 gtagacaggt gcgcaggtaa tatcgattcc tagttttttc aatgtttgtt catcattttt
    23401 attgaccatc acagtagagt gggcttgtgc tccctcaagt tcagctagtt tatcataagc
    23461 aacttgagcg attggattcg ttactgcact gattgataaa gcgatcaaga cttcattgat
    23521 gcttagtgca tccaaagagg aatgaagtgt ctttgatttc aatccttgta ttgtttcaag
    23581 aactaatggt gacaataaag gaatgtcatc tgcgatatgg gctaaatgtt ttactgcatt
    23641 taagataact gccgctgacg catccatcaa tgaacttgta cgcccggtaa tgatgacttc
    23701 atctggtaat tcgattgcaa tcacagcaga aggatctttt gtaggacttt gcgcttgaat
    23761 cttatctgca tagtcatggg cagggactac cacgtttcgg tcttcaggac gtaaaccgac
    23821 ttcttccata atcagtttga ttcgattaac tgcttcttcg tcgatcaatc cttttttgaa
    23881 gtcgcattct gtttggaaat aacgacggat gatttcttgt ttagaggctt cttggacaac
    23941 ggtatcatcc acgatcccaa atcctacacg attcacaccc atgtcagtag gagattgata
    24001 aacagattct ttaccagtaa ttttttcaat aatacgtttg attacaggaa atgtctcaac
    24061 atcccgatta tagttcacag ctactttatt gtaggcatca aagtggaagg aatcgatcat
    24121 attgacatct tttagatcga ctgtggctgc ttcataagca atgttcagcg gatgtttcaa
    24181 cggaacattc catactggga aagtttcgaa cttggagtaa ccagctgcat gtcctttttg
    24241 gctttcatga tacagttggt tcaagcaagt agcgagcttt ccacttcctg ggccaggagc
    24301 tgtcactaca acgattggtt tagttgtttc aatatactca ttttttccaa aaccatcttc
    24361 tgagacgatt ttttctacgt tgactggata gtcttcgatt tctgagtgtt tatagacctt
    24421 gatgtttcgt ctttctagtt tattgataaa gactttcgtg gcaggctgtc cactatagcg
    24481 agtgataacg acactgttga ttaataagcc ataaccacgt aattcatcga ttaaccgcaa
    24541 gatatccata tcataagtaa tcccataatc accacggatc ttattacgtt caatatctcc
    24601 agcatagacg caaatcagta tttcagcttg atctttcaat ttttgcagca atttgatttt
    24661 cgcatcttct tcaaatcctg gaagtacacg tttcgcatgc ttgtcgtcaa ctaacttccc
    24721 accaaattct aaatataatt tgtcatagtg gttcactcgt tccaaaatat acgctgactg
    24781 ctcttcaata tatttttctg aatcaaaacc aatttttttc actaggttcc ctccttaagt
    24841 actaaagcat tttttcattt ttttgaggat tcgtcaactg gaagagcgaa attcctcaaa
    24901 aaaacaaaga aaatggttta aaccaacttg tctagatagt tattcattgg tatgatagtt
    24961 tttgataagg aaggtgctac atgaaaaaaa gacagaaaaa gaaaaacgca tataaacact
    25021 atatccgttc gatttttacc ggttatgaaa gaatgcttga agatcccgaa ttggaacaat
    25081 tgacgtttac ttatctaaac gaagaaacgc agctaactcg tgatgagcat cagcgcattc
    25141 atttcacaac aagagatctg cctagtaaat aaaaaataag ataaaggtca tcggattcga
    25201 ttctgtccta tcagatggaa agactagttg atcgaaaaaa aggctgtacc aaattttggt
    25261 gcagcctgtt ttatggagca ttgcccgctc ttttttataa caatttttta cagaaaatgg
    25321 tactattgaa gtattaataa gtgaaaaaca gatgagtaag aggaggcaag tagtatgaaa
    25381 gagccagata caaatgcaaa ttatgagcca gacgaactag aggagacgaa cgcggatcag
    25441 gatacaggac aagaatcgtc ttctttttac gatatcaaag aaaaagcaag catgaatctt
    25501 tcccaattat tagaagactt acaggctttt gaaaaagctg tccgcgaaga agatatccct
    25561 gagatctacc gtttatacaa tggccgattg aatgaagaat taaaaagaag ctcaaatgaa
    25621 aatcacgaga tcgatcaatt gctgatgcgt aagatccatg atcgtttttt acaggaattt
    25681 ccttttatgg aacaagtaga aaacatctcg ccaacgatga gctattacaa aatcggcact
    25741 tattaccatg atcggcccac ggtcggtatt gatgcaagtc tgccagaaat ttttgttttg
    25801 cctaagatcg atgaagagtg ggagaaatat tcccaaccgg agtcaacgga ctatgacaaa
    25861 aaaatcaatg agctagacgc aaaggtgatc actgctcaga cagaaattga acgtttagat
    25921 gaacaaataa aagaaatcaa tcgacaaatg actgatttga acgacaataa aggattcttg
    25981 aatcgtaaaa aaatcgagga agaaattcaa gaattagaga agaagaagca agtactatct
    26041 aatgaaaaat taggctggct tccttatatt gagacgcctg aaacaatcca gcagcaaaaa
    26101 gaagcattga agcaagaagc aagagcagac cagcttcgtg cagccatcgt agaaaaagaa
    26161 caaaggcaaa tcagacgcta tttcggtagc aaagagggat tcggtcaagc aatccatgaa
    26221 ttcttgatga attatcttgg caatgagaat cctaataatc aatctaatga aggaggttcc
    26281 gagtatgaat gaacacgatg aagcagaatg gacaaaaaat gaagaacaaa cagaagatca
    26341 tgaagaaacg aacacttcat tggaagaaca aacaagtaat cctctggatg acacagaaga
    26401 aatccctatg ctagaggaaa caaaaataca tgaaaaagaa aaagtaactc cgacgaatac
    26461 gaatactgca gaaaacaccc atgcttacga aataactgaa aatcccgggt atttagaagg
    26521 aaaagagcaa gatcatcaag atatcgtcag tttgattgaa acagcggaag cagaattagc
    26581 ggatttacgt atgagaaaag atttgatcga atcagatttt ccacaattat tagaatcata
    26641 tgatcgtttt atattgaatg aagaaccaat ctccacgatc ggaaagacaa gcctgatcga
    26701 atacttcacg tatgaattac tgaaaccgtt gaatgatatt ggtctggaac ggtcaaattc
    26761 agcttatgac acatggaaaa caagacattt ttcgatcaat tatacgttga atggacaaaa
    26821 cgaattagtc ttttcctttg tattaccgac catcgatcaa cgataccaag tggaatcgat
    26881 gagtctccta aacgtatcac cagagacaat ggaaatcaat gtccaagatg accgggtgct
    26941 ttctttgatc cgacactggt cagtggatcg agtattctct gcagggcaga tcacgatatt
    27001 caatcacaaa ttgaatcaat tattagccca tgcaagaaca cttggtttta ccgttaatca
    27061 gacattacta gacaatacga agccacttcg tttgaatctt cagagcgaat ttgaattgac
    27121 gaatgaagta ttggatgata tttttatcgt ggcaatgaaa aatccatcct acgatatgga
    27181 aaagatcaac gaaactactt atcaagtacg attagataaa ggacagtctt taacgataac
    27241 aacaaacgaa aaagatcaaa cagtcttgtc gatctcatct ggagactacc cacgatctgt
    27301 cattgatttc tttatcaatt acgaattctt ggtcccatta atggtgagaa agtcctgatt
    27361 ggataagttg gattaccaga tgtcattcag ataattctgt tccaaaggac ttgcggcagt
    27421 tatccagaat aattgtcaca agtcttttct gtatgtacta aatggacgtg aataacttgc
    27481 tgatgaagga gggatttcat taggaatgtc cacaaataaa atagcacagc ttagcttatt
    27541 gtctgctgct tgtgtagcag gtaggatcgc ttttcaattt attccgaatt tccagccaat
    27601 gactgctatt tttttattta tagtacttta cttaagctta aaagatgcgt taattgtcat
    27661 gagtctttct attgcgatca gcagttttta ttttggtgtt ggaccatggg tcatcggcca
    27721 atggatcagc tatactgttg ttctgagttt gttttcaatc gtctgcttga gaaaaacagt
    27781 gcaaaggaat ctatggttaa agggagtctg tttttttctg gcgggcatgg tttacggaat
    27841 aggtatgacc gtttttgata cgctgttata tcgtttgcct caaccttgga tctattacct
    27901 tcaaggggtg tcgtttgatt tgatgcacag tataggcaat gttgtgttct ttcttgtttt
    27961 tctgccagtc gtcaaacgtt tttacaatca ttcaggagga aaaaatgaaa aaaataattt
    28021 atagtttgct tgcaatgagt gtcagtatac tcattttgac aagttgcagt acaacctata
    28081 cacaatcggc ttccaaccaa aaggaggaag taaaaacatc gcaaaaagca acaatcacgt
    28141 tacaagaaaa tggtcaaaac ttttcggaaa aagaagtggt cttcaaaaaa ggcgatgatt
    28201 tactgagtat tttgaaacga aattttgaag tcaaagaaga taacggcttc atcacttcgc
    28261 ttgaagggca ctcacaggat gagaaaaatc aactctattg gatgtttact gtggacggga
    28321 aaagtgctac tactggtgcc aaagagatca aattgaatga tggacaatcg gtcattttca
    28381 atttgtctaa actataagtt tcataaaaaa ctgttaaagg gcgaatattg ccataattca
    28441 gtgagttcag ttgatgaaag aaggaaaata acatggaaaa ttttaaaaaa agcggaatgt
    28501 tttccgtaat tgctgctcta ggggctaata ttctagttgc tatcagcaaa tttgtcggct
    28561 atgcaatcag tggcagtgcc gccatgctaa acgaaagcat ccacagtatc gttgattgca
    28621 gcaatcagat tttgctgtta ttcggtgata aacaagccaa taaaggacag agcgagttgc
    28681 atcaatttgg tgaaggacga gcaaaatact ttttcagtac aatcgtcgca atgatgcttt
    28741 tcttcggcgg tggggcactt ggggtgatgg aagcttttga aaagttgacg catccatccc
    28801 atgaagtggg taatccgatc attgttatag caatactgtt atttggtttg gtaatcgaag
    28861 gaagttcgtt acgcgtggca atgaaagaaa tcaaagaatt gaatactgaa aaactttcca
    28921 cgatgcgctt tttgcgcgaa agtcgacaca gtgaaatact gattattttt acggaagact
    28981 tgtgtgctgt aatcggtttg gtcttggctt tagcaggaac aatattgact tatgcaaccg
    29041 ggattgctgc atttgatgcg atcagcggat tgctgatcgg tttcttgctg atgggagccg
    29101 ccattttcct agcaaaagaa ttttatagtc tgatcatcgg tgaaagcgtg acagcaagcg
    29161 atttatcaaa aatcaagtta gcttttgaac gtcctgagat cagtcggctg atcgatataa
    29221 aaacagttca tttaagtcca acggagattt tagttgcagc aaaagcagat atcgttggta
    29281 acaaagagaa tgaagcttac tcggtcatca atgatattga aaaaaatatg cgtcagtctc
    29341 ttcctgacaa gaagatgtat atatacattg aaacagataa atatgaccca aattattctc
    29401 gtaaataaaa cgtttattca tgaattgaag caaaaatgag aaaaatataa tcttgttttt
    29461 aaataaaacg gctacaattt agcgaattta tgttacaatg agtccgctgt ttatttgttg
    29521 taattaaagg agatatttta atgaacaaaa agaaaagtgc gccatggctt caagctttgc
    29581 atgcagtgat gccactttgt ataagctata taccagtagg attagcttgc ggagtattgt
    29641 tgcaaaaagt tggatttgat cctctcttat caggactgtt atcgattctg gtcttttccg
    29701 gtggcgcgca gtttttagtt gcatctatgc ttacgacgca agcatcattt gcaacgacat
    29761 tattgatggt tttcttttta gaattacgct atacattatt aggagcaagt cttgccggtt
    29821 ttatgaaaaa agaaaagcgg acgtttttgg ctgtcttttc tcaatcactg aatgatgaaa
    29881 actatgcggt gaactactta aagttttcaa ctgacccttc ctggaataaa agaaaggctt
    29941 tatatgtcaa ttggttctca atgactgcat gggccggtag caatatgatc ggtaattttt
    30001 tcggctcggt gattcgagta gatgctgacc ttgtccattt tgctttgacg gccatgttca
    30061 ttttcatgtt tatcatgcag atgaaaagcg cactactgat ctttacaggt ttattctcag
    30121 gcgtattagg ggtcgtattc atggtattat tccaaaatac tttcggattg attgcagcaa
    30181 cagtcattgc ttcatttgcc ggatttatgc tggagaaggc attcaaaaaa gaaaaagcta
    30241 agaaactaag agaccgcggt aaagatgtca atgagccgct cttcaacttc ccggaacagg
    30301 aggccaatca ccatgactaa actatatttg ctacttttag ttgcttgtct gttcatcgtg
    30361 gcatatatcc cgcgcttgtt cccgatgctt tattttactc atcgaaaagt gccatcctgg
    30421 ttcagtgact ggatgaaata tgtaccggtg gcattatttg cggcgttagc ttttaaagat
    30481 gttttcatta ctcatgaaca tctggatatt gcttggaata tcaaaatagc tgcaatgatt
    30541 ctagtagcag gcgtggctta caaaacacgt tcgatggcgt tgtcagtttt gacaggactt
    30601 gcgtctgtct ttttgctgtc tatgctttaa actgttttat tttattaaaa acagagattg
    30661 cgacataagt tgttcaaact gatctatccg aactgcattt gcaacagtca attctggatg
    30721 caagtttatt cgtagggata aaaaagatta gtgttggctt atgatgcaag ctctttttta
    30781 ttttaggaat tagagaggga tttgtccttc tatgattttt tgctataata gggaagatgt
    30841 ttattttatg aaagaatagt tgagggaatt taaatgaata aaaaattgat gcaaggaaca
    30901 ttttggctaa cctttgctaa tcttctatgt aaagttttag gggtagtcta tttgattccc
    30961 tggctaagta tgatggggaa caatcaagat ggtatgcttg ctacgacatt gtataatgtt
    31021 ggctatcttc cttatggatt gttcctgatg cttggtacag tcggatttcc aaatgcgatt
    31081 gcaaaaaaag tagctgtagc cacaaaagaa ggagataacg ctgcttgtcg gctgattttt
    31141 agaagtacga tcaatatcat gtttgtcatc ggtatcgcct ctgctgcact gatgtattta
    31201 tttgctcctt tactagccgg aatcagtccg attgctaacg ttaataatgg gatactagct
    31261 atcagaagtt tatgcccatc tttgattgct atcccaattc ttagtgcaat gagaggatat
    31321 ttccaaggaa aaaatgatct gcggccttat ggaacttctt tgattatcga acaggctgtg
    31381 cgagtgatcg ttatcttggc tggtacttat tacttgcgcg tattgactga cggaaccata
    31441 ttagaagctg tattgatcag tacagtcgct tctttcttcg gtgggttagc tgcgattatc
    31501 catatgtata tcgtcggacg cagaaaagac ttcttcgaac ttaaagattt tttgatttcc
    31561 agccgttatt tcgaaagaga aaaccgatct gcctctgtct ctatcattcg ggaaacacta
    31621 ccgtttattt ttgtcggctc agtcattact atcgttcaaa tgatcgacca agtaacgatg
    31681 aaaccactgc ttcacttttt ccgtccagag atcgcagatc agcaactgga attcttattc
    31741 agccgtgctt ctgtcaatcc aaataaattg acattgatcc tgatctccat ggtaggaaca
    31801 gtagctataa gcagcttgcc aatattaagc acgatgaaga aaaaagatcg gatacaaatc
    31861 gaaaaaacag taggagatag tttttcgatt gcgttattga ttcttcttcc ttcattgact
    31921 ggaatgtctt tattagctgg accactatac acattgtttt ttggctatga tcctgaaagt
    31981 gtcggatatt tccaaatggc tttgctagct tctttgtttt tctctttatt cacgatccta
    32041 tctacgatgc ttcaatcttt gaatcaccat cgttttgcga tccgattaac tgtagaagca
    32101 attatcctga aagtcatctt ccaagtaatc ggattaggat tagttggcgg atacgggatg
    32161 agcttatcaa atggcttagc attcggtatt gtctttgttc gcggctatca ctttatgtgc
    32221 aaagaatacc gaatagcacc tttagcaaag atcagcaatt ttttcttaaa aactttccgc
    32281 tccaccctga ttatgcttgc agtatgtttt gccgttttct ttttgctgaa tcagcgattg
    32341 tcaatggaat caaaaagcta tgcaatgatt tattgtgtgg ctgtaggaag tattggcggc
    32401 ttagtatttt tattttctca attcggaaaa aatgggtttc gaatgttgaa aaatgttaaa
    32461 catcatagca gaagcaaaga ataggttaac taatcgtttt ttgctgtcac ttgaaaaacg
    32521 gcaactcgta tgattacgct tgtcgttttt ctattttcca attttagttt catatctaat
    32581 cttacttatg atatagttat aaggtctgga aaaattcttt gaaacgagga aattaaacac
    32641 atgaaacttg cagtagttac agatagttcg gcatatctgc cggaacgtat caagaaccat
    32701 cccgacttat tcgttattcc aatccctttg attatggatg ggaaaattta taacgaagga
    32761 atcgatatcg aagcggatga atattatgga cttttaaaaa acagtaaaga ttttcctact
    32821 acttcacaac ctgtcgtagg ggaagttttg ggtttatatg aggaattgaa agagaaaggg
    32881 tatgacacga ttttaagtat ccatctttct tcagggattt ctggctttgt caacacatta
    32941 tttgcgatga aagatgacat cagcggagta acggtcattc cttatgactc taagatcact
    33001 agtatgccga tgggacacat ggtagaagct gccttagact taaatgatga aggtcagtcc
    33061 cttgatgaaa tacttgcaca tatcgatcga atccgagata atacctatgc ttatctgatt
    33121 gtagatgatt tgaacaattt agtacgagga ggacgtttga caaatggtgc agcgctgatt
    33181 ggcagcttat taaaaatcaa accgatcctg acattttctg aagggaaaat tgttttattc
    33241 gaaaaaatcc gttcaagtaa aaaagcattt gctcgagctg aagaaattat tggtagaaga
    33301 aataacgaaa tacaaccgcc agtcaaactt tacgtgatac atgcaaataa tttagaagta
    33361 gcgattgcgg agaaagagaa attgcagaaa aaatatccaa atgcagaaat cgaaattggt
    33421 catttcggac cagtcattgg tacacattta ggtgaaaaag ccatcggcat tgccatttca
    33481 gcgcaataat tccttttatt caaaaaacat accaaaaata
//
LOCUS       DARBEG010000024        32168 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000024 DARBEG010000000
VERSION     DARBEG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.024"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(407..766)
                     /locus_tag="SCK20_001109"
     CDS             complement(407..766)
                     /locus_tag="SCK20_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG1145189.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="SCK20_001110"
     CDS             complement(796..1137)
                     /locus_tag="SCK20_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145190.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="SCK20_001111"
     CDS             complement(1134..1808)
                     /locus_tag="SCK20_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1145191.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCK20_001112"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCK20_001112"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG1145192.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="SCK20_001113"
     CDS             complement(4322..5512)
                     /locus_tag="SCK20_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1145193.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="SCK20_001114"
     CDS             complement(5533..6060)
                     /locus_tag="SCK20_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG1145194.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="SCK20_001115"
     CDS             complement(6107..8896)
                     /locus_tag="SCK20_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1145195.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="SCK20_001116"
     CDS             complement(9043..9243)
                     /locus_tag="SCK20_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1145196.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="SCK20_001117"
     CDS             complement(9696..10016)
                     /locus_tag="SCK20_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145197.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="SCK20_001118"
     CDS             complement(10519..11637)
                     /locus_tag="SCK20_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145198.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="SCK20_001119"
     CDS             12108..13415
                     /locus_tag="SCK20_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG1145199.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="SCK20_001120"
     CDS             13536..14735
                     /locus_tag="SCK20_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1145200.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="SCK20_001121"
     CDS             complement(14762..16030)
                     /locus_tag="SCK20_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG1145201.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="SCK20_001122"
     CDS             complement(16200..16841)
                     /locus_tag="SCK20_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG1145202.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="SCK20_001123"
     CDS             complement(17250..17753)
                     /locus_tag="SCK20_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1145203.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="SCK20_001124"
     CDS             complement(17876..18640)
                     /locus_tag="SCK20_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG1145204.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="SCK20_001125"
     CDS             18748..19023
                     /locus_tag="SCK20_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG1145205.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="SCK20_001126"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="SCK20_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1145206.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="SCK20_001127"
     CDS             complement(20579..20980)
                     /locus_tag="SCK20_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1145207.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="SCK20_001128"
     CDS             complement(20994..21803)
                     /locus_tag="SCK20_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1145208.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="SCK20_001129"
     CDS             complement(21790..22680)
                     /locus_tag="SCK20_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1145209.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="SCK20_001130"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="SCK20_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="SCK20_001131"
     CDS             complement(23348..24289)
                     /locus_tag="SCK20_001131"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG1145210.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCK20_001132"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCK20_001132"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1145211.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="SCK20_001133"
     CDS             complement(25274..26470)
                     /locus_tag="SCK20_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1145212.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="SCK20_001134"
     CDS             complement(26488..27216)
                     /locus_tag="SCK20_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1145213.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCK20_001135"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCK20_001135"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1145214.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="SCK20_001136"
     CDS             complement(28628..29086)
                     /locus_tag="SCK20_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1145215.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="SCK20_001137"
     CDS             complement(29083..30546)
                     /locus_tag="SCK20_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1145216.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCK20_001138"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCK20_001138"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1145217.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32168
                     /locus_tag="SCK20_001139"
     CDS             31602..>32168
                     /locus_tag="SCK20_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1145218.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQ"
BASE COUNT         9382 a         6921 c         5511 g        10354 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaa
//
LOCUS       DARBEG010000025        30392 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000025 DARBEG010000000
VERSION     DARBEG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.025"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(32..229)
                     /locus_tag="SCK20_001140"
     CDS             complement(32..229)
                     /locus_tag="SCK20_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG1145219.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(623..2350)
                     /locus_tag="SCK20_001141"
     CDS             complement(623..2350)
                     /locus_tag="SCK20_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG1145220.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2343..3536)
                     /locus_tag="SCK20_001142"
     CDS             complement(2343..3536)
                     /locus_tag="SCK20_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1145221.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3723..4367)
                     /locus_tag="SCK20_001143"
     CDS             complement(3723..4367)
                     /locus_tag="SCK20_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1145222.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4461..4595)
                     /locus_tag="SCK20_001144"
     CDS             complement(4461..4595)
                     /locus_tag="SCK20_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG1145223.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4597..6729)
                     /gene="feoB"
                     /locus_tag="SCK20_001145"
     CDS             complement(4597..6729)
                     /gene="feoB"
                     /locus_tag="SCK20_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG1145224.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6726..7199)
                     /locus_tag="SCK20_001146"
     CDS             complement(6726..7199)
                     /locus_tag="SCK20_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG1145225.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7600..7824
                     /gene="nrdH"
                     /locus_tag="SCK20_001147"
     CDS             7600..7824
                     /gene="nrdH"
                     /locus_tag="SCK20_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG1145226.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7983..10142
                     /gene="nrdE"
                     /locus_tag="SCK20_001148"
     CDS             7983..10142
                     /gene="nrdE"
                     /locus_tag="SCK20_001148"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG1145227.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10192..11157
                     /gene="nrdF"
                     /locus_tag="SCK20_001149"
     CDS             10192..11157
                     /gene="nrdF"
                     /locus_tag="SCK20_001149"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG1145228.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11246..12385)
                     /locus_tag="SCK20_001150"
     CDS             complement(11246..12385)
                     /locus_tag="SCK20_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1145229.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12694..13407)
                     /locus_tag="SCK20_001151"
     CDS             complement(12694..13407)
                     /locus_tag="SCK20_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG1145230.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13661..14362)
                     /gene="nagB"
                     /locus_tag="SCK20_001152"
     CDS             complement(13661..14362)
                     /gene="nagB"
                     /locus_tag="SCK20_001152"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG1145231.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14596..16128
                     /locus_tag="SCK20_001153"
     CDS             14596..16128
                     /locus_tag="SCK20_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1145232.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16319..17521)
                     /locus_tag="SCK20_001154"
     CDS             complement(16319..17521)
                     /locus_tag="SCK20_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1145233.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17603..18292)
                     /locus_tag="SCK20_001155"
     CDS             complement(17603..18292)
                     /locus_tag="SCK20_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1145234.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18398..18766)
                     /gene="rplL"
                     /locus_tag="SCK20_001156"
     CDS             complement(18398..18766)
                     /gene="rplL"
                     /locus_tag="SCK20_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG1145235.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18819..19322)
                     /locus_tag="SCK20_001157"
     CDS             complement(18819..19322)
                     /locus_tag="SCK20_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG1145236.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19526..19653)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19732..20421)
                     /gene="rplA"
                     /locus_tag="SCK20_001158"
     CDS             complement(19732..20421)
                     /gene="rplA"
                     /locus_tag="SCK20_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG1145237.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20525..20947)
                     /gene="rplK"
                     /locus_tag="SCK20_001159"
     CDS             complement(20525..20947)
                     /gene="rplK"
                     /locus_tag="SCK20_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG1145238.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21290..21958
                     /gene="sdaAB"
                     /locus_tag="SCK20_001160"
     CDS             21290..21958
                     /gene="sdaAB"
                     /locus_tag="SCK20_001160"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG1145239.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21971..22843
                     /gene="sdaAA"
                     /locus_tag="SCK20_001161"
     CDS             21971..22843
                     /gene="sdaAA"
                     /locus_tag="SCK20_001161"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG1145240.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22934..23953)
                     /locus_tag="SCK20_001162"
     CDS             complement(22934..23953)
                     /locus_tag="SCK20_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1145241.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24126..25031)
                     /locus_tag="SCK20_001163"
     CDS             complement(24126..25031)
                     /locus_tag="SCK20_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1145242.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25090..25635)
                     /gene="nusG"
                     /locus_tag="SCK20_001164"
     CDS             complement(25090..25635)
                     /gene="nusG"
                     /locus_tag="SCK20_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG1145243.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25736..25906)
                     /gene="secE"
                     /locus_tag="SCK20_001165"
     CDS             complement(25736..25906)
                     /gene="secE"
                     /locus_tag="SCK20_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG1145244.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25930..26082)
                     /gene="rpmG"
                     /locus_tag="SCK20_001166"
     CDS             complement(25930..26082)
                     /gene="rpmG"
                     /locus_tag="SCK20_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1145245.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26244..26888
                     /locus_tag="SCK20_001167"
     CDS             26244..26888
                     /locus_tag="SCK20_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1145246.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26944..27447)
                     /locus_tag="SCK20_001168"
     CDS             complement(26944..27447)
                     /locus_tag="SCK20_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1145247.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27460..27506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28044..29009
                     /locus_tag="SCK20_001169"
     CDS             28044..29009
                     /locus_tag="SCK20_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1145248.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29108..30280)
                     /locus_tag="SCK20_001170"
     CDS             complement(29108..30280)
                     /locus_tag="SCK20_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145249.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30267..>30392)
                     /locus_tag="SCK20_001171"
     CDS             complement(30267..>30392)
                     /locus_tag="SCK20_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145250.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9266 a         6252 c         5394 g         9480 t
ORIGIN      
        1 atgaacaatt tgcttagcat tttttttcat tttagcactt ccctggaatt gctccaccta
       61 aaaaaccacc tatagacatt cctgcaatac aagtagttgc cttggcccaa tcgaccgtac
      121 atttattttt agtgcaatac actccatttc cataatattt tccactatga gtggtacccc
      181 catagataag ttgtgtctct ttaatagaca aaatttttaa atgtttcata atctaatcac
      241 ccctaaatta atgtttttag actattattc taacattcat taaatttcaa aaaacaattc
      301 cttcttgaaa cgtagcaaaa taattcctga ataagaattt aatcactttt aagtatataa
      361 actatgtata ttctttaata ttttttgaga atgcacttcg tacataataa actttaatca
      421 cattagtctt tcatacctac gatcgatctc tttgactcgc acccataaat tcataatagt
      481 ttgtcggcgt ctttggacgt ttcgaagatg agctacgcat tctctttttt tatagacaaa
      541 aaagatagag acgaacatgg agcttccaaa tttggatatt tgatccaatt ttggaagctc
      601 catttatttc gtgtctgtct ttttattttt tccagctttc gactaattct tggttctctt
      661 tgatccagtc ttttgctgct tgctgtggat cttttccatt gttgatttcc aacataactt
      721 tttccatatc tttttctgac caatggaaat tgtctaatac tttgtaagct tctggctggt
      781 cttctttcaa tccttttcga accatcgtat ggatctgctc ttcgcttccc attccgtttt
      841 caggatcttc taagtatttc agatcatatt tggcaaacat ccaatgaggt gtccatcctg
      901 taactacaat tggttcgtgt tttttgatcg cttgacctag tgcaacggtc attgctcctg
      961 aagaagatgt ctctactttc cagtctttca agttgtcata tttttggatc gtgttttcag
     1021 cagccgtaac tacaccagct cctggctcga ttcctatgat tttcttacct gcctgatcgg
     1081 ttaactcatc gatcgaattt acatccatgt aggctggcac tgctaaacca acttttgctc
     1141 ctgtcaaatt ggcacctagg tcttcgactt gatctttata ttgtgcatat tgtgaaccat
     1201 gagttttagg tagccaagct gaaaccatgg catctgactc gccatttgaa acagatttcc
     1261 acatgattga attatctaat ggcgtcatcg ttacatcata gcccatttgt ttcaatactt
     1321 caccgactac atttgttgaa gcaacttctg tatcccattc aacataagat aagttgatac
     1381 gttttgtttc tttggcagat gaaaaactcc ctataccaat cgcacctaag atcactacta
     1441 ctacagcacc aatgatcagt ccttgacgtt ttttactttt ctgtgttgtt gctcctgcag
     1501 cttttttatt gtttggctgg ttcaaatgct gtgtaaaacg gtcaatgatg attgctaaga
     1561 taactaatgc aaccccgttg acaaatccat tcccgacttg tgctctttgc aatgctgata
     1621 atacgccgcg tcctaagccg ggtgcaccga tcatagaggc aataacgacc attgaaagtg
     1681 acagcattgt tgtttgattg atccctgcca tgatcgtact tttagctaaa ggcaattcta
     1741 gtttgaatag tttttgacga cttgtgctcc cgaatgaatc agaagcttct actaattctt
     1801 taggaacttg tcgaatccct aaattcgtaa aacgaacggt tggtggcaga gcaaagatga
     1861 ctgaggcaaa tacccctgga accatcccga taccgaaaaa tgcaactgct ggaatcaaat
     1921 aaacgaatcc tggcattgtc tgcataaagt caaggatcgg tttgataata ctttgggctt
     1981 tactgctttt tgccatcaag atccctaatg gtaccccaat gatgatcgag atcacactag
     2041 ataaaagaac aagtgtgaca gtattcatca aatcattcca tagaccttgg ttataaataa
     2101 accatagacc gattaatgtg aacaacgtaa gtcctggacg tttcttagaa ataaagaaag
     2161 ctgctaccgt caataacaag ataaacaaag gagctggaat cactaacagt aaatttgtaa
     2221 tccccgacat cactgcctgt ccaatcgttt gtaaaaatga aaataggcca gaaaaagttg
     2281 tcgtaaacca ttctgtgata ttttctaccc agtttgccac tggtaattga tagttcaata
     2341 gattattcat gttcactcac ctcgcttgtt tctgctaatg cttcaatcac actacctcgg
     2401 atgacgacac cttttaattt tccatcctct acaactgcaa gtggagtcgg tgagtcataa
     2461 ataatattga aaatatcact taccaccatg tctttcccaa tcaccgtcac attttcatct
     2521 aggaagtcga tcaatggacg tttttgcttc ctagcttcta acgcttgatc tgcagtgatg
     2581 atccctttca ggtgtctctt tctgtcaaca gccatcagca tgctgacttc ttcattacgc
     2641 atccttgtca atgcaacgtt tggtccatca gattcgatat tcgtcgtcaa tgcaggtacc
     2701 atgatgtttt gagccgtaag cactttggaa cgatcgactt cttccacaaa ttcacgtaca
     2761 taatcatttg ctggattggt caatatttct tcacctgttc cgatctgcat gatttcgccg
     2821 tctttcatca aggcgattct gtccccaatc cgtaaagctt cattcaaatc atgggtaatg
     2881 aaaataatcg ttttttgaac attggcttgc aggtccagta attcgtcttg catttctcgc
     2941 cggatcagcg gatcaagagc cgagaacgct tcgtccatca gcaagatctc aggatcattt
     3001 gctaaagctc tggctaggcc aacccgttgt tgcatcccac cagataactg actaggatat
     3061 tggtctttaa atgaaagcaa actagaattt tctaatgctt tttctgcttt ttcttgtcgt
     3121 tcttctttag gtacaccacg gacttctaac ccgtattctg tattttccaa gatcgttcga
     3181 tgtggaaaca gaccgaagtt ttggaaaacc atattgattt tatgacgacg gacttctcgc
     3241 aattcttctt tgcttaactt cgatacatct tctccatcaa tatagatact gcccgaagtt
     3301 ggctcaatca gacgattcaa tagacggatc aatgtggatt tcccactccc ggaaagcccc
     3361 atgatcacga agatttctcc ttcttttacg tcaaaattca cgtcatacac accaaccgtt
     3421 gcaccggttt tctttaaaat ctccgttttc ggctggtgat cattcatcat atctaaagcg
     3481 gcttgtgtct ttttaccaaa tatctttgtt aaatgtttta cttctacttt taccaaacaa
     3541 attctccttt tttatgtaaa ttcatttttc tttctgaaaa aagtgaccac actatgttaa
     3601 catcatgtag tcactttgtc aaaaatgaaa gaaatttgct tgtccaaact gaaaaaaact
     3661 tggctaaatc tttgtaaata aaaggtttag ccaagttaat atttttttaa tatttcacac
     3721 aatcattctg caagctgatg ctccgggtaa aaaaagtttg tcatacgctg tagtgtatat
     3781 tcatttccaa taaaatgaac gatgtcccct gccgtaaatt tagcataggg gccaggtgaa
     3841 acaagcagtt cttcattatg agaaattgcg ataactgtgg cgccagtcgc ctgccagata
     3901 ttcaactcgc taattgaacg gtccagatgc attgcttttt ctgataaaga taattcaaac
     3961 ggaagtaaag gattcatctt atgtattcgc ttcgtttgat tgaccagctg atccaataat
     4021 tgagaaaaat gattcagctc atcttgttgc ttctccacac ttgccatgat ttctgctttt
     4081 gtatcttgga tggattgtac atcttgatat tgcaatagga aatcttttgc tttttccttc
     4141 gaagcaacaa atgccccact tccatgccgt acttccatga ttccaagatc taccagcaca
     4201 ttgatagcct tgcgggtagt ctcaggagaa gcattgtacg tactggcaag tgttgagcgg
     4261 gcatggatct tctcacccac tttatatcgg ttttcaacga tacgttcggc taaatcaaca
     4321 gcaatctgct ggtagcgcgg gcggacgatt tttttctttc cagacatttt ctttcctctt
     4381 tcttttttta ctttccctca tcttactatg ctctagcaaa ataagcaaaa aaggggagaa
     4441 tagcctcttg caacgactga tcatcgcttg atctgttcct tttttactgg gcaagctgtt
     4501 tgacagtcat cacaattctt accggatttt aaacgagtat agacaattgc gcctgcactt
     4561 ccaaagatta acacacttaa taagaatgtc gccatcctat tcctctcctt ctaatgacaa
     4621 tatagtataa aactggttct tggattttgg ctgacggaac aaaacataaa cgagtcctat
     4681 catcagtaaa atagcaagat acgtccctaa cgctacctgt ccaccctcca ataagtgtcc
     4741 caactgataa atcacaaaac taactaaata agctaaaccg cattgatagc ctatggcgat
     4801 ccaagtccat ttcttggcat tcatttcccg atggatagcc ccaattgccg caaaacaagg
     4861 cgcacaaagt aaattgaagg ctaacaatga ataagctgct gcgggagtat atgtgtgttg
     4921 tagcacttgc cagatttctc tgccattttc ggatacttcc gcatgaccaa acaaaatacc
     4981 aaatgtgcca ataatatttt ctttagcgac caatcctgta atcgctgcga ctgttccttg
     5041 ccagtttccc caaccaagcg gctgaaaaac aactgccagg attcgcccga aaaaggctaa
     5101 gatactttgg tcttcagaaa caggttgagc ataaaaatta taagaagagc tgaaccagat
     5161 cacgatacta gaaacaaaga taatcgttcc tgctttttta acaaaagact tgctgcggtc
     5221 ataggtttgt cttaggaccg ctgaccattg aggaaaatga tatgcgggca attccatgat
     5281 aaaaggagct gtatctccag caaacaaacg tgtttttttc aagctgattc cagagaaaat
     5341 aattgcagtc ataccaagaa aataggcaga aggagctacc caagattgat gaggaaaaaa
     5401 ggcccctgct actaaggcaa taatcggcaa ctttgcagaa caaggcatga aggttgtgac
     5461 catgatcgtc aaacgacgat cttgctcgtt ttcaatcgtg cgactggcca tgacgcctgg
     5521 cactccacat cctgtagcaa tcagcatagg aatgaaggat tttcctgaaa gaccgaattt
     5581 cctgaaaagt cgatccatga caaaagcgat cctcgccata tatccgcagt cttccagaaa
     5641 gctcaagcat aaaaacaata taatcaattg cggtagaaat cctaacactg ctcccacgcc
     5701 tgcgatgatg ccgttcaaga tcagctcctg catccatccc gctacttgcc attcagctaa
     5761 caatctgcca acatgttctg gtaaccatgt accaaacaat gtgtcattga tccaatctgt
     5821 ccccattgtt cctactgttt gaatagccag ataatagatc agccacatca caaatgcgaa
     5881 gatcggcaag gctaaccaac gattcgttgc aatctgatcg attttgtcac tcatcgataa
     5941 ttggaaagaa tcatttgaca caacacagag tgtacatagt tgggtaataa attcataacg
     6001 ttcattgacc agaatcgtct cactatcgtc gtccaatagt ttctctgtta actggatcag
     6061 ttgctcgatc tctttttgct gagaaactgc tatctcgtaa tcctctctta ccttctgatc
     6121 tcgttcaaaa tatttcattg cataccagcg tttcttgccg ctttgttctc ctagtacatc
     6181 ttcaatttca tgtaaagctg cttctaaacg tggatcatat gtacagggaa caggaataga
     6241 aggccgcttt tttgcagact cctgaatctt ttcgattagt ttttcgattc cttgcttttt
     6301 caatacgctg attggtaaaa ctggtacacc aagaccatag gaaagctttt ctagattgat
     6361 tttttttcct tcttttttga tgacatccat catattcaga ccaatgatca taggaatgtc
     6421 taaatccaat aactgcgtag ttaaataaag atttcgttct aaattggtag catcaataat
     6481 gttgatgatc agttcaggct gttcctgaac caaataatct cttgcaatca cttcttctgg
     6541 tgtataagga gacaaagaat agatgccggg taggtcctga atatgaatgt tcggattttt
     6601 tttcaacgat ccttcttttt tttctactgt tacgcccggc caattcccta catattgatt
     6661 cgaaccagtc aagagattga acaagctagt tttcccgctg tttggatttc cgactaacgc
     6721 gatattcatt ctttttcctc cctttgtatc aagattagtt cggcctctga ctttcgtaac
     6781 gtcagttcat accctcgcac tgtcagttcg attggatctc ccagaggggc tagttttcgg
     6841 ataactattt ttacatttcg cgtcaatccc atatccatta accgtcgctt cactgcgcct
     6901 tgtccgtgga ttgctacgac gatcccagtc tctcctgctt ttaattgatc aagagaagtc
     6961 cagctttcag aggaagcagt ctttttattg acaaagattt gacctagtat ttcccgactt
     7021 actgccactc gatgactttt taccagtaaa atggcattgt tgcctgataa attgacgacg
     7081 acgattttac ttccggcaac gatcccgata ttttgcaaat ggcgttttgt tttttcttct
     7141 gtttcgattc gttcgacttg ataaacgaaa ttctgctttg cctgcgctaa ggtctgcata
     7201 ttgattcctc tcttgtgaaa taaatcatgt attaaaatca ttcattatat gttctaattt
     7261 tcttacgtgt tacgcaaata aatgagaatc attctcacat agaatactct cggtaattga
     7321 gaatgtcaat tatacttgtg atattatgag cgaatcttta gtattgatag ataaaaatga
     7381 gaaaaaaata tttcttcata gttttccaca attttttctc tattaaattt gctattttcc
     7441 cacacgatac ttgttttcag ctcttttttc aaaaacacaa aacttagtat tccacagttt
     7501 ccacaacaca atatgtagtg tttgttgttt gactttttta ctaagagttt tatactatct
     7561 attatagata ataagaatgt gttagaggag gcactcacca tgaacgtaaa aatcttttct
     7621 aaaaataatt gtatgcaatg taagatggtc aaacggtttt taacagaaaa tcaaattgct
     7681 tttgaagaag tcaatatcga tgaacagcct gaagcagtag actggttgaa agcacaagga
     7741 ttccaaagtg ttcctgtcat tacttctgat gcagaaacag ttatcggatt ccgtcctgat
     7801 cagttgcgta aacttgccag ctaacatgac taaaagaatc aaaacgtgat acatggattt
     7861 gaagggcttg agacagcacc agtttttgtc gcaaacttcc aaatcctttt ttaaagctta
     7921 aaaacaatgc aatgatttgc aatcaagcat catcaaatac gaaatgaagg ttgggcgtac
     7981 gtatgagtct taaaaattta caagatgtca gttatttcaa gctgaataat gaaatcaatc
     8041 ggccagtcaa tggtcaaatc cctttgaata aggataaaga agctttagtt gccttttttg
     8101 aggaaaatgt caaaccaaat acgatgactt ttctatctat tatggaaaaa atcaattact
     8161 tgatcgatca agaatatata gaagcagaat tcattcaatt gtattcacca tcatttattg
     8221 aaaagctata tcaatttttg gacgagcaac atttttcttt caaatctttt atggctgctt
     8281 acaaatttta ttctcaatat gcgttgaaaa ccaacgacgg ttccgcttat ctggaaactt
     8341 atgaagatcg agtagccttc aatgcgcttt actttgcgaa cggggatgaa gaattagcac
     8401 ttgctcttgc agacgaaatg atccatcaac gatatcagcc agctacacct tctttcttga
     8461 atgctggacg taaacgtcgg ggcgaattgg tgtcttgttt cttgactcaa gtcactgacg
     8521 atatgaactc tatcgggcgc tcaatcaact ctgctttgca attgtcccgt attggtggcg
     8581 gcgtagggat tactttatca aatcttcgtg aagccggcgc tcctatcaaa ggatatgagg
     8641 gtgctgcaag tggtgtcgtt ccagtcatga aattattcga agatagtttc agctattcta
     8701 atcaattagg tcagcgtcaa ggagctggcg tggtttattt gaacgttttc catccagaca
     8761 ttattgcttt cctttcagca aaaaaagaaa atgcagatga aaagattcgc gtaaaaactc
     8821 tttctcttgg cgtgatcgta cctgataaat tttatgaatt gacacgtaaa aacgaagata
     8881 tgtatttatt tagtccatat agtgtcgaaa aagaatatgg tgtcccattc tcttatgtaa
     8941 atattacagc tgagtatgat catctagttg caaatccaaa cattcgcaaa caaaaaatca
     9001 aagcgcgtga tcttgaaaat gaaatctcaa aattacaaca agaatctggc tatccttacg
     9061 taatcaatat tgatacagcg aaccgcgaga atccaatcga tggcaagatc atcatgagta
     9121 atttatgttc agagatttta caagttcaaa cgccttctgt gattaacggc aaacaggaat
     9181 atgaagtatt aggaacggat atctcatgta acttaggctc gacaaatatc gtgaatttga
     9241 tgcaaagccc taactttggt caatctgtgc gtacgatgac tcgtgcattg acatttatta
     9301 cagatgcatc tgatatcgat gtcgtaccac cgatccaaaa cggaaataag ctgaatcata
     9361 cgattggatt aggtgccatg ggcttacaca cttatcttgc aaaagaacaa atggaatatg
     9421 gctcagagga ttcattagac tttacaaata tttacttcat gctgttgaat tattggacac
     9481 ttgttgaaag caacaatatc gctcgtgaaa gaaaacaagt tttccataat tttgaaaaat
     9541 cagcttatgc tgacggcact tatttcgaaa aatacgtaac tggagaattc cagccaaaat
     9601 cagaaaaagt caaagcattg tttgaaggca tttttatccc aactgctgaa gattggaatg
     9661 ctttgaaaca agcagtaatg aaagatggac tgtatcatca aaatcgcttg gcagtagcac
     9721 caaatggctc aatttcatat atcaacgata cgagtgcaag tattcatcct atcactcgca
     9781 tgatcgaaga acgccaagag aagaaaatcg gtaaaattta ttacccagcg ccttatctat
     9841 ccaatgagac gattccgtat tacacttctg cttatgatat ggacatgcgt aaagtaatcg
     9901 atgtttatgc aatggcacaa caacacgtcg atcaaggcat gagtttgact ttattcatgc
     9961 gttctgaaat cccagaaggc atttatgaat ggaagacaac aacgaagcaa acgacacgtg
    10021 atttgaatat tttaagacat tacgctttcc acaaaggaat caaatccatt tattacgtac
    10081 gtacctttac tgatgatgca gaagaaatcg gaagcaatca atgtgaaagc tgcgtgatct
    10141 aatatcatag atagattcgc ttattcatat tttaattaaa ggagcattct catgtcagaa
    10201 acatattatg aagcaataaa ctggaatgaa attgaagata tcattgataa atccacttgg
    10261 gagaagctga cagagcaatt ttggctagat acacgtatcc cactttcaaa tgacctagat
    10321 gattggcgta cattgtctca attggaaaaa gatacagtag gacatgtatt tggcggtttg
    10381 acactgctag atactgtcca atctgaaagt ggcatggatc agttgcgtaa agatgtccgc
    10441 actccccatg aagaagccgt tttgaacaat attcaattca tggaatccgt tcatgccaaa
    10501 agttattctt ctatttttag tacactgaat acgaaaaaag aaatcgaaga aatttttgac
    10561 tggacaaata cgaatcctta tctgcaaaaa aaagctgagc gtatcaatga aatctacaaa
    10621 cacggtacac cgctagaaaa gaaaattgcc agcgtctttt tagaaacatt cttgttctat
    10681 tcaggttttt atactcctct ttattattta ggaaacaaca aattggctaa cgtagcagag
    10741 atcatcaaac taatcatccg agatgaatct gtacatggaa cctacatcgg ttacaaattc
    10801 caattaggat tcaacgagtt acctgaagca gaacaagaaa cgttaaaaga ctggatgtac
    10861 aatcttcttt atgagcttta cgaaaacgaa gaacgttata cagaagaatt atacgatcca
    10921 atcggctgga cagaagaagt caagacattc ctacgctata atgccaacaa agcattgatg
    10981 aacctaggga tggatccact ctttccagat actgccaatg atgtcaaccc aatcgtaatg
    11041 aacgggattt caacaggcac aagcaaccat gacttcttct cacaagtcgg aaacggctat
    11101 ttgttaggtc atgtagaagc gatgaaagac gacgattatt tgattggatt ggaataagga
    11161 tttattttaa ttcaaaaatt tttcgaaaaa taaaaagagt ttagcgtaca gatattttaa
    11221 atgtacactg aactcttttt tatgcttatt cacttgtttc ttccagcact tttggactgt
    11281 ctaacttttc cgaaacatgt tccattcttc tcaataaata gattttccgg atatttaata
    11341 ttatcgtctt aatcactgca tagacaggta tgcaaatcag catgcctaat attcctgcga
    11401 cacttcctgc tccaatcaat accaagatga tcgtcaaagg atggatattc attgatttcc
    11461 caaataacaa cggcttgatc aaatttccat ctaattgttg gatcactaaa atcgacaacg
    11521 ccatatacaa agcttgaaat ggagagataa acaaaccgac aatgattgct ggtaccgctc
    11581 caataaaagg gccgacatac gggataatat tcgtgattcc gcaaatgacc gcaagcaaca
    11641 agctataagg ctgatcgaat attctcattg ccaaaaagct cattacacca ataatcaaag
    11701 catctaagat cgtactgctg atataggcag ataatgtttc gtttaattct tttaccgttt
    11761 ggcgtaactc tttacgaatc gctttcggaa agaagtgaga caacgcatca ataaatttgt
    11821 gcccatcctt gaacataaat agcaaaataa atggaacggt aaataacaaa ataaaaaatt
    11881 gcatcataaa tgaaatgatt tttgagacac ttgcagataa actgactaat atgatattga
    11941 acaaattact aagagataaa tttgctgatg ctaattgttc ttccaaattg aaatttttaa
    12001 actcttcccg ctgcgccaat tcatttaacc atttgcttgt ttcatctgca tacattggca
    12061 agctctgggt caattgaatt gtctgttcca ttaactgagg cactacattc atgacagtta
    12121 aaacaactaa aatgattacg accaaaaacg tcaataaaaa accgaacact ctcgggatcc
    12181 gatgcttttc gagaaaaaca acgattggat cgaacatata atagataaaa ccagcaacaa
    12241 gcaatggtaa aaaaactgcc gaaagcattt tcaatattgg actaaaaata ttcggcattt
    12301 gtaaaataaa gtacacgccg atgatcgtga gtactagctc cactgtccag aaaaataatt
    12361 ttgattgttt aaaacgtgat tgcatacatc tcctccaagg tcctttgata cttctattct
    12421 atcatttaac aaagagttac gcgctgagat tttcatttgc ttaaaaaaaa caaaaaaaat
    12481 aaacatattt taatgagtag acgagtactt aaacaaaaac acggtttgga gtagtgacaa
    12541 aaattatact gcagtccctt tttccaaata aacggtgttc aaaagcttgc atcttcgtaa
    12601 taagcccagc caaacgaaaa atataaggaa ctatttttat ttggctgggc ttattacttg
    12661 atgtaggaca tctattttac aacctcagat catttattgc gcaatactga ctaaatgcaa
    12721 ctttgtgata tttttgtgta cggacaactc ctcgatcaca tcggcataag tgagcccttt
    12781 tggaagatca atcgtataga tatttttgta cactcggtag tcatcaacga attttacgtc
    12841 aaaattcaca tcttcgatta cgatattttt ctcttcaaaa taatcggtaa gaaaatcttt
    12901 ggtttctttt ctatggataa attggatttc taacttttta gtcgtaggta catggatcac
    12961 gcgtttcact actgtgagtg caatagcaat cccgataaaa ctgatgatcg caatggaata
    13021 gtaacccatg ccgatggcga tccctaatcc agcaattgcc cataaagaag cggcagtcgt
    13081 aagtcccgtc accgattgtt tcgtgacgat gatcgttccc gcgcctaaga aaccgacacc
    13141 actcaccact tgagcaatca gccttgcttc atctgaacga atcgttccag caagctgtgg
    13201 atactctaaa gcatctctta atgcaccact agcgatctca acttggatca atgcaatgat
    13261 acatgctccc atacaaacta aaatatgcgt acgcatacct gctggacgat ttttatattg
    13321 tcgttcaaaa ccgattgctc cgccaaatag catagcgatc cctaaacgta aaataatctc
    13381 tggaattgtc aataccatat cattcatcta aacaccctcc agtccaatat atcatgtctg
    13441 aaaggagcag acaaaaaaag tgcaattttt acctcttacg aaaaaaggac tgtgacaaaa
    13501 tttttgtcat agtccttttt atgaataaac ggtgttcaat cagctgaccc tcggtattac
    13561 tcaacaaacg gcaaaatatg gggagctatt tttcctttgt cttctctaat actcaggtca
    13621 aaacgctgat ttcacaacct ctttagccta ctattgaaaa ttacaactta cttgctgctt
    13681 cttcatcgat gatcacaaca acattgtcgt gattttgcaa tacacttgct ggcaaatgat
    13741 tcgttacagg tccttcaatc atccctttga ttgcatcagc tttgctttcg ccaaatgcca
    13801 tcaagacgat ttctttactt ttcataattg aaccgattcc cattgaaaca gcttttgttg
    13861 gtacatcttc tgctttatcg aagaatcgtt tgtttgcatt gattgttgat tctgttaagt
    13921 caactactga agtcgttccg tcaaatgaag caccaggttc attgaaaccg atatgtccat
    13981 tttgcccgat tcctaagatt tggatatcga ttggatgtgc atcgatgatt tgatcatact
    14041 ttttgcatgc tgcttccaga tcttctgctt ttccatctgg tacaaacgtt tctttgaatg
    14101 gttttttatc aaataattgg acatccatga aatgacggta gctttgttca tctgaaccgc
    14161 ctagtccaac gtactcatct aagtttacag aagtcatttc actgaaatcc aaatcgcttg
    14221 aagtcatttc tttatataaa gtaactggtg tgcttcctgt tgccaatcct aatacttttg
    14281 cacctttgtt cataccttct ttgatgattt caaatgcttt ttttccgcct tcttcagcgt
    14341 tttttacacg aatgatttcc atagtgattg ctcctttata ttttggtata gcccaattat
    14401 agcattcttt ttttcttttt acaataatta gtatagacca aattgcattt ttttcatttt
    14461 cttgacaata aataggaatc cgtgttaggt ttcataagat ggttcaatca aacaatccat
    14521 gttgcatgaa aaccaagcaa gtgattcact gtggacgctt tatgtctgca gtttttttat
    14581 gaaaaaggag ttattatgag tatactgaca ttaaacaaca ttacccagca atttggcgac
    14641 aaaatcctct atgaaggtgt cgatctgcaa ttaaatgctg gcgaacatct tgggctgatt
    14701 ggacaaaacg gagccggtaa aagtacattg attaaaatca tcactggtga gatcttacca
    14761 gatgacggac agatccattg gcaaaaaaat attcacaaag gttacctcga tcaatatgtg
    14821 gaagtcgatg aaacattaac aatcgaagaa tttctaaaaa ctgcgtacgc aaaagaatac
    14881 gaaaaggaag aaaaaattgc ttttctttac caaacatata gcgagacctt cgatgattcc
    14941 ttgctggaaa aagctgggaa actccaaacg gaattagacc aaggagtgtt ttatcaaatc
    15001 gacaccttag ttgctgagat gagcagcggg ttggggatcg atgtcttagg tttagattca
    15061 ctgttgaaaa atctaagtgg tggacaacgt tcaaaggtga ttttagcaaa actactttta
    15121 gaaaacccag atgttctgat cttagatgaa ccaaccaatc acttagatga tcagcatatc
    15181 caatggctga cgcagtttct tcaggatttt gacggtgcgt atatcgtgat ttcccatgac
    15241 caagaatttc ttgatcagat caccacccat atcgcagaca tcgagtttgg aaagattact
    15301 aagtatactg gccatctaaa acaagcattg aaacaaaagg aacaaaatcg cgaaagctat
    15361 ttgcgccaat atcatgctca acaaaaacaa atcgaaaaga ccgaagctta tattcgaaaa
    15421 tacaaagccg gttcccgatc aacgatggca aaaagccgac aaaaacaatt agataaaatc
    15481 gaacggctga cgcctccagc ttcagcaagt aaagctgtct ttgattttcc attctcgcct
    15541 attgtcacga cattagcagt ggaaacaact gatttagtca tcggatatga tcgaccgcta
    15601 cttgcaccca tcaatttgac catccgcttt ggtgaaaata ttgccattcg aggcttcaac
    15661 gggattggaa aatctacttt attaaaaaca ctgatcggtg agattgaaaa aataagtggc
    15721 gatttccact tcccagataa cacaaaaatc aattactttt cacaagatct gtactgggaa
    15781 aatccattag aaacaccact acaatattta agcagtcaat ttccaaaagc gactatcaaa
    15841 gaattacgtc gccaactagc taaagcaggg ttagttaatc aattagcaag tgaacctctc
    15901 actacattaa gtggtgggga acaaactaag gtgaaacttg cacagttgac aatgaaccaa
    15961 ggtaatttac tgattctcga tgaaccgacg aaccatatcg atcaagaaac gaaagaaagc
    16021 ttacaagacg ggatacagaa atttccagga acagtaatca tcgtctctca tgaacaagaa
    16081 ttttatcaag atcttgtgca acgagtcatt gagattgaac caaaataaac gtttgattga
    16141 atagttgaaa aaaaggagct gtgacaaaag ttttgtcaca actccttctt ctgaataaat
    16201 ggtggccaat tagctgacct tcggtattag ctcaaaaaac ggcaaaatat gaggagctat
    16261 ttttatttgg ctattcttat tacttgatgc agaacagctt ttcacaacct cttttcgtct
    16321 attttttatt ttcgagattt ctctttcctt taaaaacaac tgtttgaaag aagaacgcac
    16381 atccttgaaa aacaagcccg ctaaccgtta cacctaacca gccatactgc tgccacattg
    16441 tcgtccctag caatgaacca gccgcacctc caataaagta cataaacata aatacagtat
    16501 tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg gcgacttgac
    16561 caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc aaatggcttc
    16621 cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata tacacgattt
    16681 ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact gctccagaca
    16741 aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg ggactgctga
    16801 tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa aatccactga
    16861 ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt ttaagtgaat
    16921 caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc cagtaaagca
    16981 gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc caagaaaaaa
    17041 gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt aaaccgctaa
    17101 gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca ccgtaaggaa
    17161 tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat gcaaataacg
    17221 taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct gccattcgaa
    17281 tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt aaccctaacg
    17341 catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta acagataaac
    17401 tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca acaacgccac
    17461 aagtgattga taatagtaaa ataagcgaag ttgttaaagg ttttttttca ttactcttca
    17521 ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg aaatagaaaa
    17581 atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt ttcttgcgat
    17641 ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc aacgtgactg
    17701 cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac acagacgaag
    17761 ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc atcgcatctg
    17821 cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac attacaccta
    17881 tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg gttttcttat
    17941 ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga gcattaccca
    18001 aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg actccaacta
    18061 ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc aagagtatcg
    18121 atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc gctctcggat
    18181 ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc actaccagta
    18241 tcagaatgat tcccaacgag atcaatatca atcggattat tttctttttc atatttcctc
    18301 ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa agcaccgaaa
    18361 tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt acagatgcgc
    18421 caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg ccttctttaa
    18481 ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa ccagttgctt
    18541 cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat tctacagtga
    18601 attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct acaggagcag
    18661 cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt tctaggatag
    18721 ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata atttcctccg
    18781 tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc tccatcttct
    18841 ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg tagtacagat
    18901 agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt gatttcttcc
    18961 aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt tgcttcttta
    19021 gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa tgcaacagct
    19081 gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc tgcacgtgaa
    19141 agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg tttacgtaaa
    19201 cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga tgcagctgct
    19261 gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat tgcttcactc
    19321 atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga aaaggcaagt
    19381 cacttttata gttactttgc tcaaccagta ttattcatca cctattcact tcgttcatag
    19441 tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc agtattgttc
    19501 atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc accgcagaca
    19561 tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag gcttgatgcc
    19621 acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat gctaaactag
    19681 ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta attagaaaga
    19741 agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat ttttcatgta
    19801 ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg ctgcaaagtt
    19861 ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga tatttccagc
    19921 tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt ttgttacgtc
    19981 cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta atactcgtcc
    20041 tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa agtcaaacca
    20101 tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac cagctgcttc
    20161 agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt taccagttcc
    20221 gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt ctacgttcaa
    20281 acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt ttgctaaagc
    20341 tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg catcttgcat
    20401 ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac ggttgtacct
    20461 cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc ttagggtgta
    20521 tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg cgcattgctg
    20581 cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct cttacttgat
    20641 cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt tcgattttag
    20701 ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg aatgaacggt
    20761 cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct gtacgagcgt
    20821 tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca ggacctactg
    20881 gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt tctactttct
    20941 ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg catatttctc
    21001 ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa caatgcacac
    21061 tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat ttttacattt
    21121 tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc tcatttcttc
    21181 ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat aacacggttt
    21241 tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa tgaaagattt
    21301 aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa gtagctctca
    21361 cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg aacagcctga
    21421 ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc acggaacaga
    21481 tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt tagctgattc
    21541 tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga gtgaaaaagc
    21601 cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc tttcagtcac
    21661 tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat tcaaaatttc
    21721 tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag gtatgattgc
    21781 taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga cagtgacacg
    21841 ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc aagtagaaga
    21901 aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt tcgattaagg
    21961 agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag cttatccaag
    22021 tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc gtagtcacat
    22081 catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg agggaaaaga
    22141 aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag atgattattt
    22201 aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca atgctgtagc
    22261 tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg ccggaagcgc
    22321 gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga cacatgaaca
    22381 gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa acaacgcttc
    22441 tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg caatggcagc
    22501 agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag cagtagccat
    22561 tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag ttgaagtccc
    22621 gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg atatggcatt
    22681 agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt accaagtcgg
    22741 acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg ttacgccaac
    22801 tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct ccttaatttc
    22861 gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta attgcccttc
    22921 tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc gtttttgttc
    22981 ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca gatgttactc
    23041 cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca actaagtcag
    23101 caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag tcgaaaatca
    23161 ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag gacgcaggac
    23221 ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc cctgcagaga
    23281 tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga agatgggtga
    23341 tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct gcataaattt
    23401 ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac gcagctaagt
    23461 taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg taaccaccga
    23521 gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct ttgttcaaaa
    23581 tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc ggtgcgcctg
    23641 gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg gaaaagattg
    23701 atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt gccaatcctt
    23761 gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa acagatgtat
    23821 gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt gtattaaaag
    23881 aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata tagccaaaag
    23941 ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt acaatatcga
    24001 ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc atcttcttga
    24061 ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct cagatacttt
    24121 tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg gttttcatga
    24181 agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc atttccgcgt
    24241 tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc atcactcaag
    24301 tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg aatattaccg
    24361 atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt tgctaactca
    24421 tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc aattgctgtt
    24481 aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata gcgtagtgca
    24541 ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta gttttctttc
    24601 agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt tttattatct
    24661 tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc tgttgcttgt
    24721 atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg atttttttcc
    24781 aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg gaaaaaaagt
    24841 gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc tttatcagaa
    24901 acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa actgataagg
    24961 actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt catacacctc
    25021 ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc gacatttcaa
    25081 gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac tggtttcacg
    25141 tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt ctgttacttg
    25201 tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa cttctacttc
    25261 tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga cttcttctgg
    25321 aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg tcacaccagg
    25381 tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa gtacgtatcc
    25441 tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag tttctttttc
    25501 ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg cacgagattc
    25561 gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat accagttttt
    25621 ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac ctcagtacac
    25681 ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta gcgagttatt
    25741 ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac atcaatccga
    25801 aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt ctgcttggcc
    25861 aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga caacctccct
    25921 tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt tacaaaactt
    25981 attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt agttgcgaga
    26041 cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct gcccccttgc
    26101 tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt caatttgtga
    26161 aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa ttaagctaaa
    26221 atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag aaaaaagatc
    26281 tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta gaaacttccg
    26341 gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga aacatttaca
    26401 aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct gtcactcatc
    26461 ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa gtattcttta
    26521 cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt tatggtattt
    26581 tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa caaggaagct
    26641 atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt gcaaaaatca
    26701 tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa gacgaataca
    26761 ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt gaaacgatcg
    26821 tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg caaaaccaag
    26881 aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt tcgcttttta
    26941 taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca atggtgctga
    27001 aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc aaaatgcttg
    27061 gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa agaagatcgt
    27121 gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag ctgtcaggcc
    27181 taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa aaccaaagaa
    27241 ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt taaagacaac
    27301 taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata caggcgcaac
    27361 taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga ttacggataa
    27421 ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt ttttttaacg
    27481 cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca gacttttcat
    27541 ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat gtaaacgttt
    27601 gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa ttcccagtaa
    27661 agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata aataaaaaag
    27721 gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc tgaagatcta
    27781 agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat gcgcttgcta
    27841 ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt ctgctataat
    27901 aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt cattcaaaaa
    27961 atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa atatcttgtt
    28021 ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc aatccatcgg
    28081 caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta aaaaataagc
    28141 atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag attgctgaaa
    28201 ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca atcgatactt
    28261 tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt gatactttct
    28321 atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga gatgattttg
    28381 ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt ttagcagctc
    28441 aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa aaaaaaggaa
    28501 tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg acaaatcatt
    28561 cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat ttattctcta
    28621 ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca ggaggaaaac
    28681 aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt tttggcggag
    28741 gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt gtcgtggtcg
    28801 aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag aaaaaacaat
    28861 tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt tcaacaacac
    28921 cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat atcacaataa
    28981 ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag ctacatctgg
    29041 aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc ctttttttac
    29101 tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct ttacccaaaa
    29161 taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca ttgaaccaaa
    29221 aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta ttattttgaa
    29281 caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc aagtattctg
    29341 ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct ttgatcccac
    29401 cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta atgatcggca
    29461 ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac gaggtgtgga
    29521 tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag taactaatca
    29581 aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc tggtttcgtt
    29641 tactgatctc tttgcgatta aatgctagat tgatgacgct gaaaccgcta aaaatggata
    29701 gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt ttcatccgct
    29761 gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt tgtgtctgtt
    29821 ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt tgcaaaaagg
    29881 tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc tgtatcttga
    29941 tcgtcggcat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga tgtaagataa
    30001 tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt ccatccaatg
    30061 taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca ggcgactctt
    30121 cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca tcaatatttt
    30181 gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa ttcgatttat
    30241 ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc tctttccatc
    30301 caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag attggtataa
    30361 aaactagcca aatcagtgta gaaataaagt tt
//
LOCUS       DARBEG010000026        29284 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000026 DARBEG010000000
VERSION     DARBEG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.026"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(41..889)
                     /locus_tag="SCK20_001172"
     CDS             complement(41..889)
                     /locus_tag="SCK20_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1145251.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1113..3053)
                     /gene="thrS"
                     /locus_tag="SCK20_001173"
     CDS             complement(1113..3053)
                     /gene="thrS"
                     /locus_tag="SCK20_001173"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG1145252.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3390..3569)
                     /locus_tag="SCK20_001174"
     CDS             complement(3390..3569)
                     /locus_tag="SCK20_001174"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG1145253.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3616..5439)
                     /gene="cadA"
                     /locus_tag="SCK20_001175"
     CDS             complement(3616..5439)
                     /gene="cadA"
                     /locus_tag="SCK20_001175"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1145254.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5717..7996
                     /locus_tag="SCK20_001176"
     CDS             5717..7996
                     /locus_tag="SCK20_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1145255.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8089..8736)
                     /locus_tag="SCK20_001177"
     CDS             complement(8089..8736)
                     /locus_tag="SCK20_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145256.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8915..9634)
                     /locus_tag="SCK20_001178"
     CDS             complement(8915..9634)
                     /locus_tag="SCK20_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1145257.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9765..10916)
                     /locus_tag="SCK20_001179"
     CDS             complement(9765..10916)
                     /locus_tag="SCK20_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG1145258.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11133..11873)
                     /locus_tag="SCK20_001180"
     CDS             complement(11133..11873)
                     /locus_tag="SCK20_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1145259.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11877..12200)
                     /gene="rsfS"
                     /locus_tag="SCK20_001181"
     CDS             complement(11877..12200)
                     /gene="rsfS"
                     /locus_tag="SCK20_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG1145260.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12257..12850)
                     /gene="yqeK"
                     /locus_tag="SCK20_001182"
     CDS             complement(12257..12850)
                     /gene="yqeK"
                     /locus_tag="SCK20_001182"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG1145261.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12834..13484)
                     /locus_tag="SCK20_001183"
     CDS             complement(12834..13484)
                     /locus_tag="SCK20_001183"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG1145262.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13494..13805)
                     /gene="yhbY"
                     /locus_tag="SCK20_001184"
     CDS             complement(13494..13805)
                     /gene="yhbY"
                     /locus_tag="SCK20_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG1145263.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13821..14930)
                     /gene="yqeH"
                     /locus_tag="SCK20_001185"
     CDS             complement(13821..14930)
                     /gene="yqeH"
                     /locus_tag="SCK20_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG1145264.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14927..15454)
                     /locus_tag="SCK20_001186"
     CDS             complement(14927..15454)
                     /locus_tag="SCK20_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG1145265.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15634..16419)
                     /locus_tag="SCK20_001187"
     CDS             complement(15634..16419)
                     /locus_tag="SCK20_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG1145266.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16412..17281)
                     /locus_tag="SCK20_001188"
     CDS             complement(16412..17281)
                     /locus_tag="SCK20_001188"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG1145267.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17282..18661)
                     /locus_tag="SCK20_001189"
     CDS             complement(17282..18661)
                     /locus_tag="SCK20_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG1145268.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18669..19088)
                     /gene="fabZ"
                     /locus_tag="SCK20_001190"
     CDS             complement(18669..19088)
                     /gene="fabZ"
                     /locus_tag="SCK20_001190"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1145269.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19113..19589)
                     /gene="accB"
                     /locus_tag="SCK20_001191"
     CDS             complement(19113..19589)
                     /gene="accB"
                     /locus_tag="SCK20_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG1145270.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19592..20827)
                     /gene="fabF"
                     /locus_tag="SCK20_001192"
     CDS             complement(19592..20827)
                     /gene="fabF"
                     /locus_tag="SCK20_001192"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG1145271.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20846..21583)
                     /gene="fabG"
                     /locus_tag="SCK20_001193"
     CDS             complement(20846..21583)
                     /gene="fabG"
                     /locus_tag="SCK20_001193"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG1145272.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21595..22512)
                     /gene="fabD"
                     /locus_tag="SCK20_001194"
     CDS             complement(21595..22512)
                     /gene="fabD"
                     /locus_tag="SCK20_001194"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1145273.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22619..22843)
                     /locus_tag="SCK20_001195"
     CDS             complement(22619..22843)
                     /locus_tag="SCK20_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1145274.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22896..23858)
                     /locus_tag="SCK20_001196"
     CDS             complement(22896..23858)
                     /locus_tag="SCK20_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG1145275.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23868..24308)
                     /locus_tag="SCK20_001197"
     CDS             complement(23868..24308)
                     /locus_tag="SCK20_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1145276.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24613..25578
                     /locus_tag="SCK20_001198"
     CDS             24613..25578
                     /locus_tag="SCK20_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1145277.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25635..25865)
                     /locus_tag="SCK20_001199"
     CDS             complement(25635..25865)
                     /locus_tag="SCK20_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG1145278.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25951..26838)
                     /locus_tag="SCK20_001200"
     CDS             complement(25951..26838)
                     /locus_tag="SCK20_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG1145279.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26927..28150)
                     /locus_tag="SCK20_001201"
     CDS             complement(26927..28150)
                     /locus_tag="SCK20_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1145280.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28167..29213)
                     /locus_tag="SCK20_001202"
     CDS             complement(28167..29213)
                     /locus_tag="SCK20_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1145281.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
BASE COUNT         8114 a         6416 c         5048 g         9706 t
ORIGIN      
        1 ttgttattct aaaaaactta tataaaaaat agtaaggttt ttatttcgaa tccataaaat
       61 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
      121 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      181 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      241 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      301 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      361 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      421 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      481 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      541 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      601 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      661 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      721 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      781 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      841 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      901 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      961 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
     1021 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1081 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1141 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1201 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1261 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1321 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1381 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1441 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1501 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1561 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1621 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1681 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1741 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1801 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1861 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1921 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1981 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     2041 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2101 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2161 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2221 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2281 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2341 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2401 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2461 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2521 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2581 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2641 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2701 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2761 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2821 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2881 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2941 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     3001 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3061 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3121 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3181 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3241 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3301 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3361 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3421 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3481 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3541 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3601 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3661 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3721 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3781 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3841 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3901 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3961 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     4021 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4081 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4141 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4201 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4261 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4321 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4381 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4441 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4501 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4561 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4621 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4681 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4741 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4801 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4861 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4921 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4981 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     5041 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5101 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5161 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5221 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5281 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5341 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5401 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5461 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5521 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5581 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5641 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5701 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5761 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5821 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5881 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5941 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     6001 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6061 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6121 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6181 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6241 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6301 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6361 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6421 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6481 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6541 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6601 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6661 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6721 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6781 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6841 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6901 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6961 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     7021 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7081 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7141 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7201 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7261 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7321 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7381 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7441 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7501 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7561 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7621 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7681 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7741 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7801 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7861 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7921 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7981 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     8041 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     8101 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8161 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8221 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8281 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8341 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8401 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8461 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8521 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8581 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8641 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8701 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8761 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8821 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8881 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8941 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     9001 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     9061 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9121 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9181 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9241 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9301 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9361 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9421 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9481 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9541 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9601 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9661 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9721 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9781 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9841 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9901 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     9961 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
    10021 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
    10081 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10141 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10201 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
    10261 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10321 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10381 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10441 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10501 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10561 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10621 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10681 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10741 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
    10801 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
    10861 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10921 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10981 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    11041 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    11101 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11161 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11221 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11281 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11341 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11401 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
    11461 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11521 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11581 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11641 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11701 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
    11761 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
    11821 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11881 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11941 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    12001 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    12061 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12121 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
    12181 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
    12241 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12301 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12361 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12421 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12481 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
    12541 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12601 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12661 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12721 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12781 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12841 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12901 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12961 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    13021 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    13081 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13141 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13201 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13261 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13321 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13381 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13441 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13501 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13561 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13621 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13681 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13741 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13801 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13861 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13921 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13981 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    14041 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    14101 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14161 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14221 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14281 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14341 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14401 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14461 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14521 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14581 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14641 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14701 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
    14761 gagacatctt gggtctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14821 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14881 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14941 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    15001 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    15061 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15121 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15181 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15241 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15301 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15361 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15421 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15481 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15541 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15601 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
    15661 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
    15721 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
    15781 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
    15841 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
    15901 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
    15961 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
    16021 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
    16081 gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
    16141 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
    16201 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
    16261 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
    16321 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
    16381 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
    16441 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
    16501 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
    16561 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
    16621 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
    16681 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
    16741 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
    16801 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
    16861 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
    16921 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
    16981 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
    17041 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
    17101 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
    17161 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
    17221 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
    17281 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
    17341 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
    17401 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
    17461 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
    17521 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
    17581 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
    17641 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
    17701 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
    17761 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
    17821 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
    17881 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
    17941 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
    18001 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
    18061 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    18121 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    18181 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    18241 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    18301 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    18361 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    18421 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    18481 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    18541 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    18601 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    18661 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    18721 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    18781 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    18841 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    18901 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    18961 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    19021 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    19081 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    19141 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    19201 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    19261 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    19321 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    19381 attatttctg gagcagttgt tgcttgttca ggtatgacat tgactggctc cgaaactggc
    19441 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    19501 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    19561 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    19621 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    19681 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    19741 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    19801 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    19861 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    19921 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    19981 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    20041 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    20101 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    20161 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    20221 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    20281 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    20341 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    20401 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    20461 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    20521 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    20581 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    20641 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    20701 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    20761 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    20821 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    20881 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    20941 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    21001 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    21061 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    21121 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    21181 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    21241 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    21301 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    21361 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    21421 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    21481 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    21541 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    21601 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    21661 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    21721 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    21781 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    21841 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    21901 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    21961 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    22021 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    22081 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    22141 tccagctggg gcagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    22201 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    22261 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    22321 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    22381 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    22441 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    22501 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    22561 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    22621 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    22681 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    22741 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    22801 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    22861 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    22921 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    22981 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    23041 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    23101 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    23161 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    23221 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    23281 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    23341 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    23401 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    23461 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    23521 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    23581 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    23641 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    23701 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    23761 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    23821 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    23881 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    23941 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    24001 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    24061 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    24121 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    24181 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    24241 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    24301 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    24361 atatcttaac ttttttcaaa atgcaacctt aaaagatcat tttttactat atgagctttt
    24421 tttaagattt tactcattta aaacaactga taaatcagca tttataattt cttttattag
    24481 aaaaatcatc tatctcacat attttgatta tcaaagtatt ttctgtgaac agaactatag
    24541 aattccctaa aacttgtcca ttatttcaaa atttgttata gtctttttat tcaaagcgta
    24601 aaaggagatc ctatgttaaa agaaaaaatg aaaactctcc ccatgctgcc gggtgtctat
    24661 cttatgaaag accgagatgg taaggtcatc tatgttggta aagcaaaacg gctcaaaaat
    24721 agagtaagca gctattttca tcagaataaa cagcattcaa aaaaagtatt acgtatgatt
    24781 catcatataa cagattttga tttcgtcgta gtagacacgg aattagatgc tttattgctc
    24841 gagtgtcaat tgatccaaca ttaccgtcct ttttataacc gacaaatgaa ttatttcagt
    24901 aattataatt atgtacatat taccaataag ggatttgtat taacagatac tcctacggct
    24961 cgaacatacg ggccttttcg tctctataaa aaaatgccgt ctattttgcg tataatggaa
    25021 gaaacttatc aaatgccttg gttgtctgaa attagtttgt tagctttgcg agtccagctt
    25081 cctgacctac aggaaatgac ttttgaacag aaaaaaaagg aactccaagg gctgtttcaa
    25141 ggccgaaata aaaagctact gacttacttg aaaaaaagac aacaacattt catctatcag
    25201 ttgaatttcg aaaaagccgg tatgcttcaa aaagatatcg aattagttac ttattttatt
    25261 agacggatcc aggaacaaaa gcagttttta agaacaccat cgctcacctt cagcatgcca
    25321 ttagcagcag atgagagtca gaaaaagcac tacctcgttt gttatggtca acttgcagaa
    25381 acaatgatta cctctggtga cgtttctcct gacttctatt atgaaaagaa agaagctcat
    25441 ttatctttga aacgtcaatt gtcaaaagaa gaaatcgatc ctgtacaaat cttgatcagt
    25501 taccgcaaaa aattagaaaa agaacagttt gaaattgaac tgttaaacaa aaaagaagct
    25561 gaaaagcaac taaattagtt gcttctcggc ttcttttagg catcttcaac ttcaaatgtc
    25621 aattatccta aaatttattg gattaagtca tagatttcca tcgctacgat atcgatatta
    25681 tcaaattcgt attgctgatg agtatctgtt tcagttaatt caaatgtatc ggtcgcttta
    25741 tcataagtta ctgtacactt ctctacgcct tctttttcaa aacggcgaac ttgcagttca
    25801 ttatctgctg actcgaccat agcctccagt cgttttatga ttgcgactaa ttgtgaatgt
    25861 gtcatcggag gtcctccttt tcttatttct gttatattgt atcaaaagca tagcctactt
    25921 gcattgattt tctctaaaaa aactcgtttt ttatgatttt ttttctggta tttttccttc
    25981 aggccaccaa agtttgatca aagcttgggt tccgtcattc tcaaaacctt tatctgccaa
    26041 ctcttcatat agtttcaatg cttgggtagt tgctggcaat acaagatcca ttttttctgc
    26101 ttcttctaat gcaatcttta aatctttgat aaaatgtttg acaaaaaaac caggtgtgta
    26161 atcttctttt aaaatacgag gagaataatt gcttaaagac cagttagctg cacttccacc
    26221 tgaaagcgtc tcaatcactt tctttaaatc aagtccgttt ttttgtgcat agacaagcat
    26281 ttccgtcatt cctgtcatcg tacctgcaat catgatctga ttagccattt ttgtatgctg
    26341 acctttccca gcagaaccat gcaatgtaaa tgtcgtacca aattctttga ataaaggcag
    26401 tacttgatca taaacggctt cttctccacc gaccatgatc gtcagtgtac cattctttgc
    26461 tcctaagtca ccaccggata ccggtgcatc taacgcatct gcgccttttt cttttgctgt
    26521 ttttgcaatt ttttctgcca ggcttggtgt acttgtagtc aaatcaacta aaatatgtcc
    26581 tgagatatct gcagaaaaaa tccccgactg tccatagtag atttcttcta catctttggg
    26641 gtaacctacc attgtaaaga gaatattact ttgttctgct accgcttttg gtgtatctgc
    26701 ccagatagct ccttctgtta ctaaaggatc agcttttgct tttgtccgat tatagacagc
    26761 tacttcgtgt cctgcttcta gtagatggcg tgcgacagca cttcccatga cgcctgttcc
    26821 gataaaacca agtttcattt tttcgacctc ctaaaaatca aaaatccttg cttctatgct
    26881 agtatacgct agaaacaagg attttttcat aaaaaatgtt tgaattttat cttttaaatg
    26941 aatttaattt tattttgatt cgttcaatcg agtcttcttc ttcttttctt cgcatctctt
    27001 cttcatgata aacaaccatg tcttgataaa attcgatcat agcttcgccg aaatgtgtag
    27061 atgaaatatc ataaagttta gcttctaaaa cttttggatc tttaggaact tcactggaaa
    27121 tataatcaat aagtgctggt gcaaagtctt cgtcggtttc aaaagttttc cctaatgaag
    27181 gatgatcgaa caatcggttc aaataagcat ttccttccac gacacaaggt gttccggctg
    27241 ccatcgcttc tgtataagta agcccttgtg tttctgaagt agaagcactg acaaaataat
    27301 ctgccgcttt ataataaagt gctacatcat tgttgtccac ttctcctgtg aactgcacaa
    27361 attcttctaa ctgcagttct gagatcaatt cctctaactc ttctacataa ggtccttttc
    27421 ctacgataac taatctggcg ttaggataag cggagatgac ctgaggaaac ccattaatca
    27481 aggcttggat atttttctca taagaaatcc tgcttaaaga aagaatcatc aaatggtttt
    27541 cctgaagacc tagtgactcc cttaatttgc tgatatcctg ctgagtgata tcagaacgtt
    27601 tgaatttttc aatgtcgata ccagtaggaa tcacgcgaag cggtgctatt acaccatagc
    27661 tgcgtaatgt atcaataaca cgttcacttg gacaaactac acctgtcgta tgatttgcaa
    27721 ataatctaga caagtatttt acatgggatt gcctgatcac ttttccttta ccgatataat
    27781 gaaggtagtc ttcatacatc gtatggtaag tatggatcag aggtattttc aacttcttag
    27841 ctaccatctt cccaagtatc cctgctccga attctgtatg ggtatgaata agatctagtt
    27901 ctagttcttt ggcaaccaaa taagcatacc acatgcctct aacaacaact cttcgatcct
    27961 tgaaagaaat aaatggtaca cttggcattc gaatcacatc tttttccaac ttatccgcat
    28021 taggatcggt cgttgtaaaa atatagactt catgcccatg tctttctaat tcttgtttta
    28081 atgttttgat cgatgtagca actccgctga cttgcggaaa gtaggtatcg gtaaaaaagc
    28141 cgattttcat gtttttcgcc tccaaactag tccttgacct gatttacttt ttttaatttg
    28201 ctagagactt tttcattcag tacactttcg tatacatttt taagttcttc accgatttta
    28261 ttgatacttc gctcttttgc tgtttgataa ccggcttgtg tcgtactcgg tagttcttga
    28321 ttggtaatct gttggatcag tcggataaat tcttcgtttg tcttccccat atagcagttc
    28381 ttgctatctt ctaaccagcc attatacact ggaatatctc gaacaagtac gttttgtttg
    28441 cttgctaaag cttctaaaac aacgatccct tctgtctctt cataagaagg gaaaaagaat
    28501 agatctgcac ctgaatacgc accttctatg atctctccac gaatgtaacc aggaaacagg
    28561 acattttcag gatgatcttc ttttacaatt ttccggatgt tccgaggaat cgagtacatg
    28621 ggaacatgac caaaccaaat aaatgtgtat tcgggcatct tcttagcaat ttctacgaaa
    28681 tcaatgattc ctttacgttc gaaaaacaaa ccgacacaaa tgattacttt ttgatccgga
    28741 gacagtttga aatattctct gaacttttgt tctttcattg gatcagggtg atatttctct
    28801 aagtcgatcc cattggaaat agcttgaatc ggcacacgga taccatagct ttccaacaac
    28861 tgttttgaat aaggtgttgg cgtgatcaga tagtccgctt tttggtacaa tcctaccaga
    28921 tattttttta cgataggaga aagctggttc gatccgataa aggaatttcg gaaatcttcc
    28981 tctgtagaat gcgcatgata gacaactttt tttccttctc ttctggcttt attgaccata
    29041 ttatgactat tgattccgta tgtattgatg tgcaggatgt cataatcttc tttttcatct
    29101 agtgtataag agataccgac ttccgtcaga gcacgttttt gatgatcaag cgcacgcccg
    29161 attccagatt ttgcaagcat cttttcactt tcaaaatata ataatacctt caccttacgt
    29221 cccccttcac ttatatagta taacatagcc tacaaactgt cactcaaact taagccctat
    29281 tttt
//
LOCUS       DARBEG010000027        28073 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000027 DARBEG010000000
VERSION     DARBEG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.027"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(205..1104)
                     /locus_tag="SCK20_001203"
     CDS             complement(205..1104)
                     /locus_tag="SCK20_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG1145282.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1114..1470)
                     /locus_tag="SCK20_001204"
     CDS             complement(1114..1470)
                     /locus_tag="SCK20_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1145283.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1825..2121
                     /locus_tag="SCK20_001205"
     CDS             1825..2121
                     /locus_tag="SCK20_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG1145284.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2644..3597)
                     /locus_tag="SCK20_001206"
     CDS             complement(2644..3597)
                     /locus_tag="SCK20_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1145285.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3620..4375)
                     /locus_tag="SCK20_001207"
     CDS             complement(3620..4375)
                     /locus_tag="SCK20_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1145286.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4372..5331)
                     /locus_tag="SCK20_001208"
     CDS             complement(4372..5331)
                     /locus_tag="SCK20_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1145287.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5324..6274)
                     /locus_tag="SCK20_001209"
     CDS             complement(5324..6274)
                     /locus_tag="SCK20_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1145288.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6424..6876
                     /locus_tag="SCK20_001210"
     CDS             6424..6876
                     /locus_tag="SCK20_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG1145289.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(6965..9232)
                     /gene="gshAB"
                     /locus_tag="SCK20_001211"
     CDS             complement(6965..9232)
                     /gene="gshAB"
                     /locus_tag="SCK20_001211"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG1145290.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9419..10417)
                     /gene="ftsY"
                     /locus_tag="SCK20_001212"
     CDS             complement(9419..10417)
                     /gene="ftsY"
                     /locus_tag="SCK20_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG1145291.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10428..11237)
                     /locus_tag="SCK20_001213"
     CDS             complement(10428..11237)
                     /locus_tag="SCK20_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1145292.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11246..14827)
                     /gene="smc"
                     /locus_tag="SCK20_001214"
     CDS             complement(11246..14827)
                     /gene="smc"
                     /locus_tag="SCK20_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG1145293.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14847..15533)
                     /locus_tag="SCK20_001215"
     CDS             complement(14847..15533)
                     /locus_tag="SCK20_001215"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG1145294.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15881..17662)
                     /locus_tag="SCK20_001216"
     CDS             complement(15881..17662)
                     /locus_tag="SCK20_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1145295.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17688..18602)
                     /locus_tag="SCK20_001217"
     CDS             complement(17688..18602)
                     /locus_tag="SCK20_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145296.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18629..19591)
                     /locus_tag="SCK20_001218"
     CDS             complement(18629..19591)
                     /locus_tag="SCK20_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145297.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19591..20535)
                     /locus_tag="SCK20_001219"
     CDS             complement(19591..20535)
                     /locus_tag="SCK20_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145298.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20536..21540)
                     /locus_tag="SCK20_001220"
     CDS             complement(20536..21540)
                     /locus_tag="SCK20_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145299.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21808..22050)
                     /gene="acpP"
                     /locus_tag="SCK20_001221"
     CDS             complement(21808..22050)
                     /gene="acpP"
                     /locus_tag="SCK20_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1145300.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22172..23173)
                     /gene="plsX"
                     /locus_tag="SCK20_001222"
     CDS             complement(22172..23173)
                     /gene="plsX"
                     /locus_tag="SCK20_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG1145301.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23222..25258)
                     /gene="recG"
                     /locus_tag="SCK20_001223"
     CDS             complement(23222..25258)
                     /gene="recG"
                     /locus_tag="SCK20_001223"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG1145302.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25375..27057)
                     /locus_tag="SCK20_001224"
     CDS             complement(25375..27057)
                     /locus_tag="SCK20_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG1145303.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27109..27471)
                     /locus_tag="SCK20_001225"
     CDS             complement(27109..27471)
                     /locus_tag="SCK20_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1145304.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27741..27929
                     /locus_tag="SCK20_001226"
     CDS             27741..27929
                     /locus_tag="SCK20_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG1145305.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7762 a         6070 c         4930 g         9311 t
ORIGIN      
        1 tatatttttt tttgtgatta tcgttcaaaa aaagaataca cgcttgcatc tagtactctg
       61 cgagttacaa atcatccgat caaataaaca ttcgctgatt attttccgca aaaaaagcag
      121 catatccacg ataaatggat ctactgctct tacttatcct tatgttttac aaggtatttt
      181 cttttacaca ttactaatgc tgtctcaata cggaacgatt gtcatcgctt gttcaaattc
      241 ctcttgcaaa tgttgtgttt gataggcctg aaaagctggg tgatgggcac gcaagaggac
      301 acgtaatttc ataggatcat agctgtctag caactgttct ttcaagacaa aatctcgggt
      361 cagctgccat tcataataat aaccaaaagg tctgcagctg taaatcgtgt gaccgatgga
      421 tttatcttca aagcgacgat gcgtatggcc aaaaactact tgtttgatat ttgaataacg
      481 gtcaagtaat tctccgaatg attctgatcc taaaaatgca tttaatttgt tccaacgctc
      541 ataaggagca gattgataca tgataaatgc ccgtttagga acgaaatgag tggataaaat
      601 cacctcaagt ttttttgctt ccagctggtc tagcaaattt tttgcagctg caagcatttc
      661 tgcattgacc tcgagatctg ttttcccacg ctggatgaac cgatcatacc aatacaaacg
      721 cttcattcgt acgattgcct cgtaatcttt caattcggaa aactgatagt cgtaccagcc
      781 attcatccct aaaagaacgg tctgctcatt taaagcaata taccgttcat ttaaaaaatg
      841 tgggtctgga aaatggtcca tcatctgttc attgggcaca tctgccaact cgtgattgcc
      901 gaaatgaaat gtcgtagata atcctttatt gacgaaaaat tcgacaacag ctaggcaacg
      961 gtctacttta ttagccgtat ctccggctag atgaagccga gtgatcttgt gcttttgcaa
     1021 aacttgccaa agctgcatca gctctgattc gccgaattta ttgatgtcca catgtaggtc
     1081 gctgatgatc gccaattttc ccattttatc cctctagtct tttcgaacaa cttgcaagtt
     1141 gccttttctc aagccgcttg gtccttttaa ttgtaaacta ttataagttg gttctaaagc
     1201 aagtgtggat ggatcatcca tgtaaagtat tgcactgtct ttgattttga ttggtccaac
     1261 tggtgtatca gccactacag aataaagact tagatctgtt tgtgtatctt ttttgacaat
     1321 gatcaatcga aatgccgtat ccaattggca actgacttgg ctttctgcaa atggaccgtc
     1381 accgttttcg atatctaata aaagttgatc gtctgcttct aatttattct tcaatacttc
     1441 ttgtgcttct tttgtcactt ctaatttcat ctgtatcacc tcatgtcata gaataatcac
     1501 tttattcatc agatgcaact aatttgtctg gttctgactg ttctcaattt attggaaagt
     1561 aagtcattcg tttattatga tttctcttat cacaaaagag acaactttcc cttttcttcg
     1621 ttcagtttct tgaacggaaa aagacccaaa aagcaacctg ttatttgttt aaccttattc
     1681 ctttcttaaa taacaaaaaa tactataaaa aataaaaaac ctttaacaac tattggaaaa
     1741 agaattactt tttgttcacg gattattaac aactcttcgg tagagtaaac ttatcaaata
     1801 aaagacttct ttggaggaat cactatgaag gaatatataa aaaacatcta ttttatcgaa
     1861 gaaacacaaa atatcgaggg ttcttatatt gaagtaaaaa cactctttgt caatgaagat
     1921 aaaaccaaag cattggatat ttataaaaaa ttagcttcaa aaaaaacgaa tagttttgga
     1981 ttgatattaa gcgagtataa aatcaaagca gaagagagtt atttctatca attattaaaa
     2041 cggtggagta agcttcccgc cgatttttat cgaaaaatgc agatcatcaa ctatcaaccg
     2101 cttgctgaga cacatgccta aatagaggtt gtgaaatcag tgctttgagc taataccgaa
     2161 ggtcagctga ttgaacattg tttagaagag attgtgacaa ctgcgatttg ctttgagtaa
     2221 taagaacgct ggacagaaaa tagcttctca tattttctag tctagtggac ttattgccga
     2281 gaagcagcag atggaacatc gtttaaagaa gagatcggta caaaataaaa atatctaaat
     2341 ttaagtttta tctaagtttc gctttgtacc tttgattcaa atcaaaggag gcgatcttga
     2401 tgctgaaaaa atggatcaca ctattttttg ccactcccca ttctagaatc aacccgtatg
     2461 atgtagataa tgaattcacg ttgaaaaaag atttctcaaa taaataaaaa ccatccgtaa
     2521 aatcatcaat acgattttac ggatggtttt tgataacttc tttgaggata ggctcttgct
     2581 atttaagaat gtaagaaaca ttattgttac atagtatgag agtttaatcc tctgttattt
     2641 atgctattta aatgcttgat tcacatcttc gatcatcagt ttcattgatt ctaaaccgcc
     2701 cccgcttaaa taccagacat ctggttcaag ggagatgatc tgctgatttt taccagcatt
     2761 cgtttgtgct accagttcat tggcagaaat atcatctttg ctgtcgtctc caccaatagc
     2821 ttttgttctg tctactacaa ataagatatc cggatttttt tccagcacat attcataaga
     2881 aacactttga ccgtgagtgg atgcttcgat ttgatcatct gcttgttcaa agccaaataa
     2941 atcatggatg aaaccgaatc tagaaccaga tccgtaggct gataattgcc cttcattcac
     3001 aagtgtgact agtgcttttt tgtcagtagc ttctgctttc tttttcgtct gatcgattgc
     3061 actagataat tcctctaatt gagttttagc ttcttcttgc ttaccaaaga tctcccctaa
     3121 agcagtgaca ttttgctgga aagattccca tggatttttc gcatctaaag aaaggaaaat
     3181 ggtcggagca atttgactta gatctttttg aaaatctctt tgtcggccag aaatgatgat
     3241 tagatctggc tgaagctgat taattttttc taagtctggt tcttttattc cacctgctga
     3301 ttctactttt ttataggatg aaagatactc aggaaggctg cttgtagctg ctccaactac
     3361 cttatctcca acaccaagag catccatcgt atctaaagaa ccattgtcga atacgacgac
     3421 tttttctgga ttatttggca cctcgatttg gtctccatta ctatctgtta tcgtcaatgt
     3481 agtttcagtt tgtgtgctgc ttttttttgt gtctgcctga ctggtgttgt tgctatttcc
     3541 acatgctgct aaaccaaatg atgctagaat caagatacct actactaatt ttttcatctc
     3601 ttctctccta atgttagttt tagaaataaa gacagaatct gcggccttca atctcacaaa
     3661 tccgtatgtc catttcgtat aattcatcca atacatcttt ttttatcatt tcttgggtcg
     3721 ttccttgcgt aaataactgt ccatctttca ttgcaacaat ttcgtctgca taacttgcag
     3781 caaagttgat atcatgaaga acaataacaa ttgttttccc gatttcttgt actaaatttt
     3841 ttaaaatctg catgatttga accgcaaaat tcatatctag attgttcaaa ggttcatcca
     3901 gtaaaatata atccgtgtct tgcgctagca ccattgcaat atacgcacgt tggagttgac
     3961 cgcctgaaag agtgtcaatc gattgatccg ctatttcttc taacccaagg tgttccagtg
     4021 attcctgaac gatttcctcg tcttttgtcg tcaattttcc tttcgtataa ggaaatcgtc
     4081 caaattctac taattctttc acggtgattt ggacatttat ttcattcgat tgtcgtagga
     4141 tcgataattt tttggctaat tcattggatt tccaagcctt tacttcgtta tggtctaaat
     4201 agatttcacc tgtatctttt ggtatcaaac gactgatcat cgacaacacc gttgactttc
     4261 cagcaccatt tggaccgata aaagcagtca atttcttttc aggaatcgtt aactgtacat
     4321 ctttaaccac tgattttcca tggtacagtt tattgacctt ttgtaaatca atcataggat
     4381 actcttcctt tcgcgaatca attttgtgac aaaataaatt cctccgacaa attcaacgac
     4441 gacactcact gttgtattcc agccgaaaat ctgctctact gcgagctgtc ctcctgtcag
     4501 aaacagcata gctaccagaa agcttccgat aaatagctga ccatgacgat aagtagaaaa
     4561 gacacgataa ctgatatttg cgacaataaa ccctaaaaaa gtaactggac ccacaagagc
     4621 cgttgatgtc cctgtcaaaa gactgacaca acataaaatc aaaaattcaa aacgatctgt
     4681 gtctatcccg aggtttactg cttgttcatt tccgagatgg aaaacgtcta gcacatgtcg
     4741 atatcgaaat aacaggtacc agcagccgat aatgatcaaa gaagcaggca aaagatactg
     4801 actctggata ttgccaaagc ttgcaaataa tctgccttga agcaaatcat attcattcgg
     4861 atccatgatg acttgtaaaa aggtactgac actaccaaaa agcgtaccta aaatcatccc
     4921 tgacatcagt aataaaaaaa gattcgctct catttttttc aaaagaaaga aaaataacaa
     4981 tagactcagc ccgaccatca gcccaacgtt caataagaaa aaaactgtac ctgtctgctg
     5041 aagcatcgtc actcctccga taaagaagaa aaggaccgtt tggacaagga catataacga
     5101 ctcaagtccc aatactgaag gagtcaaaaa ctggttatgc gtaattgtct gaaagctgat
     5161 tgtggcactg ctgacagcta gcgagacaag gataaatgca aaaattttct tactcctcaa
     5221 tgcacatgca aaatgccagt ttccatatgt atggatcgtc aagtacattc ctgtaacaaa
     5281 taaaacaaga acaagcatac caataaaaat agatggcttt tttttaagca tatcgtcgtc
     5341 cccttatcag aagataaagg aagatagccc caccgatgat tccaacgatc acactgactg
     5401 gtatttcata aggaaaaatc aacacgcgag cgacaagatc gcaaaatact aagaacaaac
     5461 tacctgctaa agcagttacg ccaattgttt tgtgcacttg atctccatat attctagaga
     5521 caagattcgg aatgatcact ccgataaaag gaatgcttcc taccatgatc aacatgactg
     5581 cactagccaa tgatactaag ccaatgccta aaaactgcat cttctggtaa tctattccca
     5641 gatttactgc aaacgattcg cccagaccaa tgatcgtaaa tcggtaagca aatagccaaa
     5701 ccaagagcaa caatggaaca acagcataca ataactcata atttcctcgt atcacagtcg
     5761 caaaattccc ttgaagccat gaagtcatat tttgtaccaa ctggaattga taagcaaaaa
     5821 aagtagcaac tgaaccaatg atattcccta acatgactcc aatcaacgga agcatcaatt
     5881 gatttttctg gaggaacaat cgcgtaaggg ataaaaaaag caatgttcct gcaaaagcaa
     5941 agcaaaatgc tgtcaaggat cggacaatca aggatgcacc aggtaggaaa atcatagcca
     6001 ctaaaatacc taaacgcgca ctatccatcg tgcccgcagt cgttggtgaa acaaatttat
     6061 tttgggtcaa atgttgcatg atcaacccac aaatactact ggtcgctccc acaaggatca
     6121 aacaaatcgt tcgtggcaaa cgagtcacta gcaagatttg taagctttgc ccatctaact
     6181 ggagcagttc tgacaattgt acattcgata ctccgatgaa caatgaaata ctacttaaaa
     6241 taactaataa tgctactagt cgtccgtagc gcatccaatc cacctttcta attgagaatg
     6301 attttcattc ttattttaag tgaatcgttg aacaaaagca accgcttttt catatgtttt
     6361 aggtattatt gggaatatgc tataatcgta agaaagacaa agagaagaag gagggtattt
     6421 tttatggcac aagatgatgt aaaaagccat ttggataagg gactttatgg tacaccttta
     6481 gtgaaccctg aagaacagca taaatattta gggacattca gggaacgctg ctatcttagt
     6541 atgactgttg ctgaaatgat ccaaccaaaa aacaaagaaa actttttaaa agaactcaaa
     6601 aaacatccag atgcttccat actcttgaat ggggctctac ccctttctgt ccaaaaccaa
     6661 tatatccagc tagcaacgaa aaacaatagc cgtttcactg tagtcaacga ttacgtcact
     6721 gcaaatcctg aggaattcgg attgctctta acagcaaaag aagcagtaaa tgaacctgtg
     6781 attgatatcg aaaaaaaata cccagaagaa aactcttcgc cagctgaaaa agaaaaacca
     6841 aaaaagaaaa aaagcttctg ggatcgttta ttttaaaacg aaaaaacaca taaattttta
     6901 catgcaaaaa aagcttttga tcagattgac atcaaatagg acaatctgtc aaaagctttt
     6961 tcttttaaaa tacttcagga tataacaatt tcaatacatt catcgtcaat cttctgcctt
     7021 ttcctgcaaa aggataaaca tgcatatgca tggctggatt gaaatttgct tcgatgatcc
     7081 catatgcttt tttatcagtt gtaggatcga tttctttatc tggtataatc aaatcgatac
     7141 cactaatctt agctcctagt gcttctaccg cagaaacggc gatttttttg taagcttcag
     7201 aaaattcatc agtcatatcg atcgaatcac cacctgtact gatatttgag ttctccctta
     7261 gataaacaat ttgattggct tggggaactg attcgatcgt caacccttgt tctttcaaca
     7321 tcaattgttc taattcacct aattggatca attctaaagg tgcacgatga ttcgttccac
     7381 gcaaagggtc actatttttt tcttcaacaa gttctttcac tgttcggata ctgtcaccaa
     7441 taacattagc agggactcgc aacatgatgg cttggacttc tccgtcgatc acaaagaaac
     7501 gatattccgt tcccggcata aattcttcta ctaaaaccga actgtcttca cgaaaagcaa
     7561 tggctaaacc tgcttgataa tcttccagac tagctccttc cttgaaaatc gtgataccta
     7621 acccgtaatt ggttgattta ggtttgatca caaatgcctg ctcggcaaat ctcggataag
     7681 cttttaccgc ttcttccatg gatgaaaatt ctgcgcctcc tggtacccgg aacccagctt
     7741 cttttaagac ttttttcgtt actgttttgt tttccatgat aagtgggacg atatagctgt
     7801 ccttacttgt catgtttgca tttttcacat attctacatg gtcttggaat tgcagacgca
     7861 aaaattgatc cgactcatct aatactttga cttggacacc tttttggatg gcatcgaaca
     7921 taaatatctg agtagaaagt tccatttcgc gaaaaccggc caactgatag ggacgttcat
     7981 ggctttcttt ataatattga tgtcccaatg ccacagcaac ttcttgctga ctcgatagct
     8041 gtgcttcttg atagagtttc gctgctaacg tttgttcagg atgatccatt tgagtcaatg
     8101 cttcctctac taaataaaga gaatccaacc aatccaattc aaccagcatt tgcttcattc
     8161 cttcaagaat ttctctacct tcttgatgaa attctgtttg atctgatggt tgttctagtg
     8221 ctactttgtt atttcgagtc gtaccttctg ctacccattg gtcacagtct tctttttctt
     8281 ctgtccataa catgtacatc aggaataatt gtaagaaccg aacttgctct ggattgatac
     8341 ccaatcttgc ataaggattc aaatcgatat tccgcaattc aatataacga acacctgttg
     8401 ttgctaaatc tgcgactttc tttccgcctc ggaaacgaag cggtgtataa aattctttct
     8461 cctccgaaag tttcccttct tcgatcattc gctcgatatc tgctaaatac tgtttcatag
     8521 aagcatagga aactttcaca tttggatgat tcgtatagcc cagcgcactg ttgcgaatac
     8581 tgcgaacagg ttcttgcgga tgtgattcat ctaaaaagta gttcttctca ctcattggtg
     8641 aagcaccgaa taaataagtg atcatccagc gatagcgcat aaagtttctc gctgttttca
     8701 aataaagttc tgttttgaat tcggaaaaat cttgaaattc ttcctgatgg ctgaacaatg
     8761 ttcttagcaa ctcatcgcca aattcaaaat tgaaatgaat accgcttacc atctgttttc
     8821 gcttcccgta ttcttttgct aaataacgtc gatacaagac atcttcgaaa ttttttaatt
     8881 ttgcaataat gatctcttcg tctttttccg gtaaggcagg tggcatactt aatggccaga
     8941 tcatctcttc tttcggtata gaacgagcag tcacatcata aacagcagcg agatactgaa
     9001 ataattcggg aatagaatcg gtaacagggg tgatcatctc tgtttgtgtt tcgctaaaat
     9061 ctgtttggat ataggggtga taggatcgat cgccaaagat tgctggatga tcggtttttg
     9121 ctagatttcc tgcaagatca acccgctgtc cttcccgttc tataccaaat cgagcttggt
     9181 cgataaaagg gcgcgcattg acatgcaata ataattgctt aaaattcatc attaagtgac
     9241 ctccaattca tatgaatcta attataatca aaaagtctaa gaaaaagaac gaaattgttt
     9301 gagaaccgct tacaaataag tataaaaatg aaggcatcaa aaacacagct atgttaaaaa
     9361 caatcattca aacaaggagc tgcaactttt gtcacagctc ctgcttttta gacatgggtt
     9421 attcttcttt tagtaatcct ttgaacaatc caacgacaaa atcatttgga tcaaacggct
     9481 ccaagtcatc gattccttca ccaagaccga ccaatttcac tggtaaatgc aattcattac
     9541 ggatagcaag tacgataccg ccctttgctg taccatctaa ttttgtcaaa acaagaccgg
     9601 tcacatctgt tgtttctttg aattgttttg cttgaaccat cgcgttttga ccagtggttg
     9661 catctaatac aagaagtgtt tcttggggtg cttctggtaa ttcccgctgg attactcgtt
     9721 ttattttttc taattcgttc atcaagttca ctttattttg cagtcggcct gcagtatcaa
     9781 ctagtaaaac atctgcctgc tctgtctttg ctctttccaa agcatcgaat acgacagctg
     9841 ctggatcgcc tcctgcattg ccacgaacaa cttcaacacc agcgcgttcg ccccagacaa
     9901 ctaactggtc gattgctcca gcgcggaagg tatctgctgc tgccatcaat acttttttac
     9961 cttcgttttt gaactgatgg gcaagtttac caatacttgt tgttttccct acaccattaa
    10021 caccgacaaa caaaataact gttagtccgt cttcttggat atttagttca tttacttcat
    10081 tgattccttc cgcttcatag agatccacaa gtttctcgat gatcgcattt tggacttctg
    10141 ccgatttttt cgcatttctg agtttcactt cttgacgtaa tgcttcggta attcgtatcg
    10201 ctgtttcaaa acctacatct gcaccgataa gtgtttcttc taattcttca aaaaagtctt
    10261 catcgactgt tcgaaaattg gcaaacaact cattcatccg ttcaccaaat gttttacgcg
    10321 tcttttctaa acccttgtcg tacttctcct gtacttcaac ttcttctgtt ttctcttcgc
    10381 cagtaaaagc acgcttgatt ttatcaaaaa atcccatttg actcccccta ctctaataca
    10441 aatttttgga tcactttggc tacgccattt tcttcattgc tggctgtcac gaaatctgct
    10501 gcttctttga cgaaaggcac agcattctcc atcgctacgc ctagtccagc atattggatc
    10561 atcggtaaat cattttcctc atcaccgatc gccatgattt cttcttgttt caaaccaaga
    10621 tcttttgcca ataaagaaat accataagct tttgtaacac cttttggcat aaattccaaa
    10681 agcatacttc ttgttttgat gatctcatac atttcatgga attctgctgg tatttctttg
    10741 attttaggat ctaattcttc ttggtcataa gcgattactg ccttattaag cacaaattca
    10801 tctgtaatat cgcttatatc cgctggttgg aattgcagca gcttattcaa cacattgtac
    10861 aaggattgct tccttggggc actaggcagc tgtaaaacca cattgtcaga caaaacatct
    10921 agtggcagat ccaattgctg agccagtttg tataagcgtt ggatatctga tacttccatc
    10981 actttctttt ccatgatttc accagtatca tttttttgta ccaaaccgcc gttgaaagtg
    11041 atgctgtagt caccttcatc taccagtcct aattcttgca gataatgcgc catcgcagct
    11101 aaggggcgtc ctgtacatag aacgatcttg accccttgct gttttgcttg tgctaatgct
    11161 tgtttattct catctgaaat cagcttttgt tcatttaata atgtaccgtc caaatcgatt
    11221 gctactaatt tgatcatttt cgttcctaca ctttcgtttc gttgattttc gcctcttcat
    11281 tgatatcttc catccgtaca gaaacgattt ttgatacacc ggattcctgc atcgtcactc
    11341 cgtacagtac atctgctgcg accatcgttc ctttgcggtg tgtgactacg ataaattgtg
    11401 tgtcatcttg gaaatcgctc aagtaacgac caaaacgttt cacgttggct tcatctaacg
    11461 ctgcttctac ctcatctaag atacaaaacg gaacaggaca gacacggata atggaaaaga
    11521 gcaacgcgat ggctgtaagt gctcgttctc ctccagaaag caaacttaga ctttgcagtt
    11581 ttttgccagg aggttgtact tcaatctcaa tacctgtttt cagcaaatca gttggttccg
    11641 tcagtactaa ttcagcttta ccgccgccaa acatatttgg aaagacaatt ttgaattctt
    11701 gacggatagc ttcaaatact tctttgaatc ttgtccgcac ttcatcatcc atctcatcca
    11761 tcgtttcaaa caattggttt ttcgctgcta acagatcatc tcgctgtgtt gctaagaatg
    11821 tatgtctttc actgacttgt tcgtattgtt cgattgaatt taagtttact ggtcccagtt
    11881 tttcaatctg ttcttttaag gatgaaacct ttgttctact gtcttcgata tcggttgttt
    11941 cttgatagtc ttgggaagct ttctcaaatg tcagctgata ttctgactgc agataggtca
    12001 aatgattatc taaaatcatt tcagctcggt ctttttggac ttctaatttg gattgttccg
    12061 acagtcgtgc tttttgctca cgattacgct ctgccaagac tgcttcaagc ttatcgatct
    12121 ccttttgcct tctatctcgc tgctctttgg cttttgccaa ctcggctttt aatgtttctc
    12181 gctgagcaga taattcgtta atttgttttt ccaaactttc ttctgtgact tcatgatcag
    12241 aaaagtctgc tgtcaatgtc gccaactgct tttctatcgc ttcttgacgt tcggttgcct
    12301 ctgctttttg gactcgggca ccgcggagct ggatctgcag atgattaaac tgctctttca
    12361 agacagcaag atctgcttgt tcttgtgctt tttgactttg tacttgtgct cttctggctt
    12421 ccatctggtc gctttcttga cttaacgatt tgatttcttc gtcgattttt tgtcgttgcg
    12481 attcaagatc tgtttgtttg tctttcaatt cgttcgtttg cttttcgtaa tcttcgataa
    12541 actgctgcag ttcacgtgtc tcaaaagttg agatttgctt ttctttttca aaacgttcaa
    12601 gttcatttat gatgttttgt agctgatttg tcgcttcttg ttcttcaaaa cgtaattgtt
    12661 cgcctcgtgt acgcaaaact tcttgttctt cggctaaacg agctgtttct tgttgcagct
    12721 cctgaacttt tttttcttgt gcttggagtt gtttatctgc ttcttcaaat tcactagtca
    12781 actgtttcaa ttcttgattt tgtacaaaca aactgccttg gtttcctcgt ttattcgctc
    12841 cacctgtcat ggatcctccg gcattcatca catcaccttc taaggaaacc actcgatatt
    12901 gatagcggat cgtttgcgcg attgcattgg cactagttaa gtctttggct aaaaggatcg
    12961 ttcctagcag gttttgaacc acagtttgga tttgatcggg ataactgact tgttcactgg
    13021 caatccctat aaacccttct acagctgctg cttgtgtcaa tatatgagcc ggtaattgtc
    13081 ttggtttgat tgtcgtcagt ggaaggaatg ttgctcttcc tcctctttgc tgtttcaaat
    13141 acgtgatggc ttgccgagca tctttttcat tttcaacgat gacatgctgg gctgccccac
    13201 ctaaagctgt ttcgatagct aacgtgaaat ctgcaggaac atcgatcagt tcagccactg
    13261 ctccgacaat acccgaaagc tgctgtttat gctgcaagac caatcgaacc ccttggtaaa
    13321 aaccaaaata gttttcttgg atttcttgaa ggcttttctt acgagcacgt agctgctgga
    13381 cttgattcat taattgatac attttaggct gttcttttgc taatgcttct tggaactttt
    13441 tcccgttttg ttggatcatt tctaacttct cttttgtttc agttaaagat gattttagct
    13501 gcgcctgctt ttgtgtcaat tcttcttttt gtaagatcaa tcggtctact gatgcttcta
    13561 cttccgattg cttggctaac gtctgcttgc tttttgctgt ttcttgtata tattgacgtt
    13621 ctagatactt cagttcgttt cctacagctg cttgttcttg catcaaatca acatattgat
    13681 ctcttaattc tgccaataat tctttcgttg atttttggta tttttctaat tcttgctgtg
    13741 tttttatgag atttgcttca gctttttgga tctctgtttc tttttctgct gctgctttca
    13801 tcaagctttc ttgaagcttt tcaaaatgtt taaccttttt ttgtgcttct gctaatgaag
    13861 tttgatattc ttgcgagctt ttttgtgtat gctttgtccg ctcctgcaaa acatcttttt
    13921 gtccttctgt ctgcttcagt ttttccgaaa gatctaataa tacttgctga tttttttcga
    13981 tcagacgatc ggcttgcgca ttttctttac gctgcttcgc caaaatggat tcttgttctt
    14041 ggatcgactc agatagtttg cctaattcta gattgaattt cgcaagctgt gcttgtttgt
    14101 tgtcccaatc tttttttgct gtcttgattt cagcaaccat caaagagaca tcggtttgag
    14161 tcagtgtttc ttttagccgt aaaaactcct ttgctgcctc actctgggcg gccaaaggag
    14221 ttaactgttc ttccaattcg tggatgatgt cttggacgcg gctgaggttg tcttctgttt
    14281 caaaaagctt ctgttctgct ttctttttcc gctgcttgta tttcaagaca ccagctgctt
    14341 cttcaaagat cccacgacgg tcttccggtt tactactgaa aatcgcttct acttttcctt
    14401 gagaaatgat tgagaacgat tcttttccta atccagaatc taagaacaat tcttggatat
    14461 cttttaatct gcagctttgt ttattgatga aaaactcgct ttccccagtc cgtcgataac
    14521 ggcgggttac actgatctca ttgtactcca atggcagata atgatcacta ttgtctaaaa
    14581 tcactgtgac ttctgcaata ttcagctgct tacgggtatc ggaaccggca aagatgatat
    14641 ccggcatttt tccgcctcgc agacttttag cagattgttc tcctaatacc cagcggatag
    14701 cctctgtaat attgcttttt ccgctgccat ttggtccaac cactgctgtg acgctatttt
    14761 caaaatcaat gactgtcttg tctgcaaatg atttaaaacc ggctatttca attcgtttca
    14821 aatacaaagc tttccctcac ttacttttac agtgctgcca atgcattttc cgccgcagct
    14881 tgttctgcta attttttcga tttcccttgt ccagtgccaa tcaattgatc atctacataa
    14941 acttcgatcc aaaatactcg ttcatgagct ggaccttctt cattgatcaa tcggtattcg
    15001 attgatacat cacctgattt ttgcaagact tcttgtagtt ttgttttatg atccatctca
    15061 tgtgaaaaag cacctgcttt gactttagga aaaatcactt tttcgatgaa tgcatgggtc
    15121 gtgtcgaatc cctggtcaag atataacgcg cctaaaaaag cttcgaatag gtcgcaaagc
    15181 aatgctggtc gcgtacgtcc tcctgaattc tcttctccct tgcccaacaa aacatattga
    15241 tcaaagtgac attctttggc aaatttcgac aagctgtctt cgcgaacgat cgcagctcgt
    15301 gtttttgtca attttccttc tggcaaatga ggatataaac ggaataaata ctgagaaacc
    15361 atcagttcta aaacggcatc tcctaaaaat tcaagtcgct cattatccga taattgcaga
    15421 ttgcgatgct cattcacata ggatgaatgg gtaaaagctt gttcaagcaa atttagatcg
    15481 tgaaaaacga tgttatagtt ttcttttagt tcttttgtca gctggttgtc cattttccac
    15541 attcctttta tggatatttt ttcgatactt ccttattata ctggtttttt gtcaaatttg
    15601 aaatggttcg gtcaaaaggt aagtaaagct ttaataatcc tagtgattga cgagaagttg
    15661 tgaaaaattc gtttagtttc gagtatgaag aaaaaatttt gtgaaaatgg ctttccatat
    15721 ttttgcataa atttgactta ataccgagaa actggacttt gaacaccgtt tattggagaa
    15781 gtcatgaaat cagcgccttg acctgaatat tagagaaata aacagaaaaa tatcaaaaaa
    15841 ccgaaaaagg acatcgccct ttttcggtca tttttagtga ttatgaacgt gaatctgctg
    15901 taacagaaac atttgcccat gggtttggta catcataaga ccaatcccag tctttgacac
    15961 ggtcgttgac tggcaatact tcattacgga acaacgttgg aatcgcaaat gcttcttcaa
    16021 atgcatattg ttgccatttt tgatatgctt ctttttggta ctcttcatca aatgctttgc
    16081 ttgaactgat atcgttgatc aattttgtat tttcttctga ttcataacga gtgtagttga
    16141 acgcagcatt tggtccccat agtccacttg gatttggatc agttcctgta ttccaagcag
    16201 cttggtaaac atcaatttca ggatcatcgt tttctaattt atcatagaaa gaattgaatt
    16261 cgatcagacg acctgttgtg tattggacat ccaaaccaat ttctttccat tgttggatat
    16321 agtaatctgc caatggttgc gctgtttccc caccggacat cgaagcaaag ttgattgtta
    16381 gtttatttcc atctttgtct tctcttaaac catcgtcatc cacatctttg tagccagcat
    16441 catctaatag ttttttcgct ttgtctagat cttgtgtgaa tcctttcatc tcatcatcat
    16501 ggtatgactg gaatactgga ggaatcaatg aagtcgcatt gcttctcaaa cctgcataga
    16561 aacgttcacc tactgcatta ttatctaatg catagcccat tgcttgacgt aatgatttat
    16621 cagccatttt tgaatctgga ttgtattcca cactctttgt atctgcattc catttaccta
    16681 acttgaatcc tagataagta taagaagttt gttcacggcc taagtttgta tagccagcaa
    16741 tttctttata ggtatcgtaa gtatctgttg gcatttggaa aatcatatca tataatttag
    16801 agttcaatgc ttcagttgca cttgccatcg gtacagattt catcgtgatc tttttcaatt
    16861 taggcgtacc attccagtag tgctcgtttg gcaagtattc tactgattct cctgcaacga
    16921 tattactcat gtaataaggg ccaaaagtca ctggattttt acgtacttga tcgcttgatt
    16981 ccatatcttt gattgctaca ccttctaacg tatgttttgg tgctgcatat gaccagatac
    17041 caccacccga ttgaagcatg cttgggttta cttctttata ttcgatttca actgttttgt
    17101 cgtctatttt tttgattccg gagattgtgt cggcacttcc atcatgatat tcttccatac
    17161 ctacgatgtt acggaaagtg tcgtcataac ggataccagt gtaatcttta ttcccaatga
    17221 tttcgtaagg gaaaatcaca tcgtccgccg ttacatcttc accatctgac catttgacgt
    17281 tgtcttttac tttgattgtc gcttttttcg tatcttcatc tagatcaagc gttgcagctc
    17341 cgtcgtctgt gattctgaaa tcgtcatcgt ttgcaaatag cggttcatga gaaggagcca
    17401 taaaagctgc atcatatgaa tcttggtaaa actcccactg gaacaatcct ttaaactgac
    17461 tgtccgttgc gaccgcagct tctaattcgc cgtcttcgat tgcatcatca ttattttttg
    17521 ttgcaattgg aaaagttaat tcctctttgt tattgccgtt gcctccagag ttgtttccag
    17581 aggtatctgt acctccgccc ccgcatgctg caagaacgac agcagacatc agcgctgcaa
    17641 atccaaaaat cgttttcttc attcctattt cccccttcgc ttctatacta gcctaaccgt
    17701 tggcgcgcat ctgcagaacg cttgaatgct tgtccaacat aattgatact caacatcaaa
    17761 actaaaataa ggattgatgc gggtaaccag atccattgcc gattcactaa gacttctccg
    17821 ttacttgcat aaccgatcaa tgtacctaaa ctaggtacat ttgccggcaa gccgaaacct
    17881 aagaaagtca aagttgtctc gataccgatg tttgccgcaa agttcatcgt caaattagta
    17941 atgattaatg aacttagatt cggcatgatt tcgcggaaca tgattttaaa atcattggtt
    18001 cccattgttt ttgaggcact gacataatct cttcttgctt cagataatgt cttgcttcgg
    18061 aataatcggg ctttagctac ccaataaaat gcactcatga tgaatataaa ggaccagaca
    18121 ttgtatctgg aaataatcga tacgaagacg ataatgatca gcatgatcgg tagaatcatg
    18181 ataaaatcaa cgatccgcat caagatgttg tcaatgatcc ctccgtagta gccagagatg
    18241 atccctaatc caaccccgat gatcgaagta aggatcgtga tcgcaaatcc gatcgtaaca
    18301 gagtttctag cacctatgat caattgtcct aatacatcac gtccgccttc atctgctccc
    18361 agaatgaact tttgtccttg tgtcgaaact gttcctggtg cagcgtattt gtcgaagata
    18421 ctaacataca tcactttgtc ttgatcggtc agcatcgcac cgataaagat cactaataat
    18481 aaaaccacta gcaaacctaa tgaaaaaata gcaattttat cttttttaaa ttctcgagca
    18541 atcattcgta tccccatagg aggaatactt tcttgaactg ctgtattgtt tttttcactc
    18601 atcgatccat tctcctttct gattggtttt attgaatccg aatacgcggg tcaacgatac
    18661 tcatgatgat atctgaaagt aatgtcccca ctagcgtcgc gattcctaaa atcaatacta
    18721 aggatgtgat cacggtatag tcccgttggc cgatggaatc aataaatagt tttccgactc
    18781 ccggataacc aaaaatcttt tcgataacta cagaaccagc aatcaacgaa gtgatttcat
    18841 accccatttg agaagccatc ggcaacgctg catttcggaa aatatgtcga ctgaatactt
    18901 tattcgtcgg tacccctttg gaacgggctg ttctgacaaa atcgagcgat ttcgaatcga
    18961 ttacttcact tcgcagatat tggattgtga cggcagtcgc caaaaatgct tgggtaattg
    19021 atggtaacag aatatatttc aaacggtcta tccattgagt gataaatcca ccttgcaaac
    19081 ctagtgtttg tgatccactt gttgggaacc agcctaaacg ataaccaaaa acaaatagca
    19141 tgatcaagcc gaagataaat aaaggaacag caaaactaaa gaagttgtag attacgacaa
    19201 tcttatccaa gattgaattt tgataccggc cagcaagcat ccccagaggc acagcaatca
    19261 aataagtaag gacaactgtc aagatcgata gatataaagt gttgatgatc cgttcgccga
    19321 tcaagtcagc aacagagcgt ttgtataaga aactttgacc aaaatcgcct tgcaaagcat
    19381 ttccgatcca acggaagtac tggacatacc atgggtcatt caagccagct gcttgtcgca
    19441 tctgttcgat cactgccgga tcctgatttg gattgatcaa accggtaaat ggatcccccg
    19501 gcatcgcttg ggctaataag aagatcagaa cacttaaaat aataacttgc gggatcatca
    19561 ataaaacacg tcgcagaatc gttttccaca ttagcttgca cctccatctt tcaacgccac
    19621 tttgtgtgta gttgtgatcg tacgcaaatc gtagactcga ccagtcgcat catagtattc
    19681 tttttggttt tggatatact cttgttctac tcttcgtcga ttttctttgt ggctttccct
    19741 attttctaca tcgatttgcg gaatagctga aagcagacgt ttcgtataga tatgtcgcgg
    19801 gtcattataa atgtcttctc ttgtaccgat ttctacaaat cgacctttgt acataattgc
    19861 gatattgtcg cacatgtgtt ttactacacc taaatcatgt gaaatgaata aatagctgag
    19921 gccgtattct tgctggattc gtttcatgaa attcaatact tgcgcttgaa cagatagatc
    19981 cagagcagat acaggttcat cggctacgat cagttttgga ttggtagcaa cagcacgggc
    20041 tactcctaga cgttgacgtt gtccgcctga aaattcatga ggatatttat atagcgcatc
    20101 ttccggcatc ccaacgatat ctagcagtcc ttttaccttt ttcttttctt cttgatcgct
    20161 taaccgttca aaatttcgga tgggttcagc aatgatatcc aacactcgct ttttaggatt
    20221 caaactggac attgaatctt ggaagatcat ctgcacatcc ttgttataac ccattttttt
    20281 gcggttgctt cgcttcgtta catcttttcc ttcatacatg atttgtccgg ctgttacctt
    20341 ttctaatccc acaacggctt ttcccgtagt agattttcca gagccagact ctcccactaa
    20401 accatatgtc tttccttttt cgataataaa atccacacca tctacagcat atacatgatc
    20461 cgttactcga ttgaagaatc cactgcgaat agggtaatga actttcaggt ctttgattgt
    20521 gattaattct gtcatctaca cctctccttt ttcgtctcta aagtggaaat gcttatagca
    20581 ggagcaacgt accaagtggt tcggtgcgac ttcatgaagg ataggctctt cttcatgatc
    20641 gcttgctggt atccatggaa tacgtggtgc aaaccgacaa cctttacgag gcatgttttt
    20701 cagagaagga acgacacctt cgatcacatg gagttctgca tcatgatttc cttcttgtgg
    20761 tatagagttc agtaaagagc gtgtataagg atgcttagga tgatcaaaca gttcttttac
    20821 acctgctact tcaacgaact gtccgccata catcactgcc actcgatcag ccatttctgc
    20881 tacgactcct aagtcatgag tgatcaagat gatccctgtt tttgtttctt cctgaaggtc
    20941 tttcagtaaa tcaagaatct gtgcttggat ggttacatct agggctgtcg tcggttcatc
    21001 tgcaatgacg attggaggtt tgcaggcgat tgcgattgcg atgatgacac gttgtctcat
    21061 accgcctgat agttcatggg gatattggcg tccaactctc gcaggattag gaatccctac
    21121 ttgttctagc aattctagtg cacgagcttg acgctgttct gcattcatct ttgtgtgata
    21181 ggaaagtcct tctttgatct ggtcttcgat tctcatgaga ggatttaatg cagacaacgg
    21241 atcttggaaa atcattccga tatcatttcc gcggatttta ttatagagcg tttcgttcag
    21301 gtcgattaaa ttcagatcgt tatataaaat ctcacctgta atttttgtgt tattaggatc
    21361 aagtaatccc ataatcgtag tagccaaggt acttttcccg cacccagatt ccccaacgat
    21421 tgctaaaatc tcgtttttgt ctaaagtaaa tgaaacgtca tctactgcat cgtaatagtc
    21481 atctttgagt cggaatcctg tatgtaggtt ctgaacttct aataattggt tatcggacaa
    21541 tcgaataccc ctctctgctt cctaaatgga agtttttctt aataaaaaag gacattacac
    21601 agaataaatc gttttataac aaaggttatt cttatctttg tgtaatgaat tattaataat
    21661 tatatagaaa accgctttac tttgcaacat aatttctgtt tatcgccaga attatcagaa
    21721 aaatgacttt tacgtgtcaa aaagattaaa aaaagaagga taaaaagctg catgcagctt
    21781 tttatccttc tttgctttta tttgaaatta tttaagatgg ctgctgatat aatctactgc
    21841 gccaccgact gtttcaattt gttcagcgtc ttcatctgaa atttccgtac cgaattcatc
    21901 ttcaagctct aaaacgaatt ccataatact aattgaatca gcttttaaat catctttgat
    21961 gttcaattca tccgtcactt tatcggtatc tatctcaaaa tgatttgaaa taattttcgc
    22021 tactttgtta aatacttctt cgcgagtcaa ctttttcacc tccaagaatc atacacttgt
    22081 caattaccta caccatggct aattttactg gcatttttca ggaatgtcca cctttttttc
    22141 aatggaacgg atcgcttcag atatccaaat actattcttc gcttttttca aattgttcga
    22201 ctaattgtcc aactacatct gtctcaagca ttgtgtggat ttggcggatc gtatagcgaa
    22261 cagcatcggg gcctgttgct ccatgagttt taatgactgg tgctttcaag ccaaacaaaa
    22321 cggcaccgcc atgtttggaa taatccattt ctgctttcaa agaacgtaag ccgtctttta
    22381 aaagcatagc gcccattttt cctttgatcc cttcatttag aatagcagat tttaatagat
    22441 tcatcatatt cattgctgtt ccttcaatag atttcaaaac agcgttgcct gtaaatccat
    22501 ctgtgaccac tacatcagcc acaccattga gtagctcacg agcttctacg tttccaataa
    22561 aattcaagct tgtttcattc tgaaggagtt cataggcttt tttcgtcact tcactacctt
    22621 ttgttgcttc tgttccgttg tttaagagtg cgacacgagg acgtccgata cctcggactt
    22681 tacgtgcata aaaagaacct aaaataccgt attgcaacaa atgttctggt ttgttttctg
    22741 cgttagcgcc aagatccagc atatcgaaac cggcaccttc tttccctact actggcaagg
    22801 tagacatcaa gccagggcgt tcgatatttt tgatacgtcc tacgatgaac aaccctgctg
    22861 ctagtagcgc gcctgtattc cctgctgaaa atacagcatc tgcttcaccg tttttcactg
    22921 cttgtgcagc aaggaccatt gatgcagtct tttttcgtcg aattgctttt actggctcat
    22981 catcactatt gattttttcg tctgtatgga taatcgtgat gtttttttca tttgttaagt
    23041 atttcttgat ttctgcttct tttccgtaaa gtaagaattc tacttctgga aaatcttgct
    23101 gcgctaaagc cacgccttct acaattgctt gaggtgcgtg atctccgccc attgcatcta
    23161 cggcaatttt cattgtttcc actccttttt ccgtcatcag tataaagtat agcacagtct
    23221 gctaatcgaa ataacgatta tcctgatttt ctatttggag gctttgcgct aaaggctgat
    23281 actcaggaag catctgccaa tcctttattt gccaaagggc tgcagcctct tctctggcta
    23341 cctccaaaac atttccatcc gtcaccaagt cagcaatcgc aaattgaggc agaccagatt
    23401 gacggaagcc aaatacttca ccaggaccac gtaattccag atctttttcg cttagtacaa
    23461 aaccattggt ggtctctgtc atgattttca tccgttccac acctagttca tttttaggat
    23521 tagcaactaa gatacagtag gaagcatcac ttccgcgtcc aacacgtcca cgaagttggt
    23581 gcagctgtgc caatccaaat cgatccgcat ccatgatcag catgacggtc gcgttcggca
    23641 cgtttacccc tacttcaatt acagtagtcg aaactaatat ctgcatctgg ttgtctttga
    23701 aggcttccat gattacttct ttttcctgat tcttcatttt accatgtaac aagccaactt
    23761 catattttgg tgcaaatagt tcacgaagtt tttcatagat ttccactgca ttttttacat
    23821 ctaatgcttc tgattcttca atcagcggac agatgacata catttgatgt cctcttgcca
    23881 gttctttttt cgtccaatcc agtacactgt ccaattgagg tgttcttacc cagcgtgttt
    23941 cgatcgggat acgtccagct ggaagttcat caatgataga tacatccatc tcaccatatg
    24001 ctgtaatagc taatgttcta ggaatcggag tcgctgtcat aaataagaca tctggtcgcc
    24061 agcctttttc acgaagtact ttccgctggt tcacgccaaa acggtgctgc tcatccgtaa
    24121 taactagacc taaattctga aattcgactc catcttggat caatgcatgt gtgccgatca
    24181 cgacgttaat ttccccttgc tctagttttt caaggagctc acgtcgttct tttgctttcg
    24241 ttgaccccgt aatcaatgcg atggttactt cattgggatc aaacagctgt tgcaagcttt
    24301 ccatatgttg ttgtgccaag atttctgtag gcaccatcaa cgcaccttgg aaacctgctg
    24361 taacggtagc gtataacgca atcgcggcaa ccactgtttt tccgctacct acatctccct
    24421 gcaaaagtcg ctgcatatgc tgcggactgc gaagatcccg acagatttca ttcgttacac
    24481 gtttttgtgc atttgtcagt tcaaaaggta gcttttgagt aaattgtttc aatttttgga
    24541 catcataaag gatctccaaa ccattcgttt ctgctttttc tgcttttttc aatccttgaa
    24601 gacgcatttg aaataaaaag aactcttcaa aaacgacacg tcgtttggct tgatggtttt
    24661 cttcgtggtc ttttggaaaa tgcatacttc ttacagcaga agctcggtca agcagtcgat
    24721 atttctccac aaggttgttt ggcagatttt cttcaatcaa tgtgccgtat tcttgaaacc
    24781 cttttttgat tagatcaact aatgtcgttt gacgaattga tttgttgacg tgatatatcg
    24841 gcgaaaagtc atccattgtt tgagaaccta aaatcttcat cccgttcaag gctttacgct
    24901 ttgcatccca tttaccgtat acggcgattt cctccgataa aatgactttt tcttttagat
    24961 aaggttgatt aaaaaaagaa acgttgaaca catcatgttc ttgcatcata cgaaattgca
    25021 atcggctttt tttataacca aatcgattca tcacaggagg cgagactacg attcctttca
    25081 aagtaacctt ttcttgatcc tggatctcgt ttagatttct ttcctgaata tcttcgtagc
    25141 gaaaaggata ataagtcagt aagtcttcaa tcgtttggat gcccaagctg gcaagactag
    25201 ctgctctttt ttctccgact cctgccagca tagtgattgg atcatctaaa gaacgcataa
    25261 atttttcctc cctcctttaa caaaaaaagg acttggtcag cgatcatacc tgatcactaa
    25321 ccgaaggcct tttatgaatg aatactgtgt tagaaagata ttctgacaca gaaattattc
    25381 agcagaaaat agataaggat agacaggctg atctccttgg tggatctcga tttccaaatc
    25441 aggatgtttt tctgttagtt tctcagcaaa ttcatttgct tgttcaactg ttccttcatc
    25501 acctgccaag atcgttacga tctctgtgtc ctcattgatc atttgttcta gtgtacttaa
    25561 cgctgttgct gtgatatctg ggttagagat caggattttt ccatcgatca tgcccaaaaa
    25621 gtctccttct ttgatctcta caccgtcaat tgaggtatct ctgatcgcgt gagtgacttg
    25681 accgctcaca acgctagaca acatttctgt catcgtttct ttgttttctt ctaaagaagc
    25741 ttgatcattg aatgaaagca tagctgtcaa tccttgggaa atcgttcttg aaggaacaac
    25801 tgctactggg atatctgcta cttctgctgc ttgatcagct gccatgaaga tgttcttatt
    25861 gtttggcaag acaatcactt tatctgcgtt tacttcttga atcgctttaa caatgtcttc
    25921 cgtacttgga ttcattgttt gtccaccact gataacataa ttagcgccta aactttcaaa
    25981 cagctcttgg acgcctttac cagcagcgat cgctacgata ccatacggga tacgtggtat
    26041 ttcttttttc gcaaactcgt tcacttcttc atcgtgttcc aaaatcgtct catgctgcaa
    26101 gcgcatgttg tcgactttga ttttgactaa tgaaccgaat ttttgtccat aattcaagac
    26161 ttctcctggg ttttctgtat gcacgtggac tttgatgatc tcgtcatcat tgaccacaag
    26221 taaagaatct cctataccat ctaaatagtt acggaattct tcgtaatcga atgtactatc
    26281 gactgtaggt ccttctccga tttttaccat gatttctgta cagtaaccaa aatggatatc
    26341 ttctgtcgcc atttgacctt ggacactgcg atgatgttct gcattgacca tatcatccat
    26401 ttctgctgga gaaggttcat atgtttcatc cgcttggaat tctccattca atgcacttaa
    26461 aaagccctca taaacaaaca gcagtccttg ccctccactg tctacgacac cgacttcttt
    26521 taggactgga agcaagtctg gagttttagc taaagcacgc tttgctcctt ttacaacagc
    26581 tgtcattact tcaacgacat catcagtact ttttgctttc ttttctccag ctttcgctgc
    26641 ttctctagct actgtcaaaa tcgttccttc tactggtttc attactgctt tatacgcagt
    26701 ttcaactcca tgagtgaagg ctgcagcaag atcagaagca tttaatgaga caacatctgg
    26761 aatttgctta gaaaatccac gaaacaattg agacaaaata acgccggaat ttccgcgcgc
    26821 acccatcagc aatccttttg aaagcattgc tgctaattct cctacttttt ctgaggttga
    26881 atctactact gccttagctc cactagtcat agatagattc atatttgttc ccgtatcgcc
    26941 atctggcaca gggaatacat ttaatgaatt gacgtattct gcatttactt gcagacgatt
    27001 cgcacctgcc tggaccattt cctggaactg gcttgcactt atttctgtta cactcaccta
    27061 gataatcctc cttcagatcg actgctgagt taccagccag tctcctgcct agtctggcag
    27121 tacacgaaca ccttgtacaa agacattaac tgaattggca gttacgccta ataatgcctc
    27181 tagattgtat ttcacttttt cttggacgtt gcgggaaact tcagagattt tcgtaccata
    27241 gctgataatc gtataaacat ctacagcaat accattatct tcttggcgga caactactcc
    27301 tcttgagtaa ttttctttac gcaaaatctc agtaatatta tctttaattt gatttttgct
    27361 ggccattcct acaacaccga acacatctgt cgcggcgcca ccaacaactg ttgcaattac
    27421 atcattggta atttcaatgg ttccagctga cgtttgaatt tttacagcca tgaatgagcc
    27481 tccttaacgg gtaaaaaact cccatttttc ttctatcata ttttatcata gcgcaacttg
    27541 aaataaaagg cagttaccca aagtactcaa aaaccttgca aaatttcagg tataaccttg
    27601 tttaatttac aattattctt ctcaattgtc aattgtatct atgaaaatgt acttgcaaat
    27661 tcccattcta tatgatatgt tagtctggta tgagctgaat tattctattg ctccaaatca
    27721 aggcaaagga ggaaattacc atggcaaaag tttgttactt tacaggtcgc aagacaaaaa
    27781 gtggaaacaa ccgttcacac gctatgaact caacaaaacg tactgttaaa cctaacttac
    27841 aaaaagttcg cgttatggtc gacggaaaac ctaaaaaagt ttgggtgtca actcgtgctt
    27901 tgaaatcagg aaaagttgaa cgcgtttaat tgaatgatac ttggaccgtt cagatagtga
    27961 acggtttttt atttaataaa aatttttaag ggtgggactt ttgattccta cccttttttt
    28021 gcgtttattt ttgttatacg aacaaattat gaagttattt attctaaatt tta
//
LOCUS       DARBEG010000028        27998 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000028 DARBEG010000000
VERSION     DARBEG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.028"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(474..1418)
                     /locus_tag="SCK20_001227"
     CDS             complement(474..1418)
                     /locus_tag="SCK20_001227"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1145306.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1714..2658)
                     /locus_tag="SCK20_001228"
     CDS             complement(1714..2658)
                     /locus_tag="SCK20_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145307.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2878..4092
                     /locus_tag="SCK20_001229"
     CDS             2878..4092
                     /locus_tag="SCK20_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1145308.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4114..6129
                     /locus_tag="SCK20_001230"
     CDS             4114..6129
                     /locus_tag="SCK20_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG1145309.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6212..7585)
                     /locus_tag="SCK20_001231"
     CDS             complement(6212..7585)
                     /locus_tag="SCK20_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1145310.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7611..7895)
                     /locus_tag="SCK20_001232"
     CDS             complement(7611..7895)
                     /locus_tag="SCK20_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1145311.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7897..9912)
                     /locus_tag="SCK20_001233"
     CDS             complement(7897..9912)
                     /locus_tag="SCK20_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1145312.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10091..10396)
                     /locus_tag="SCK20_001234"
     CDS             complement(10091..10396)
                     /locus_tag="SCK20_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG1145313.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10485..11516)
                     /locus_tag="SCK20_001235"
     CDS             complement(10485..11516)
                     /locus_tag="SCK20_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1145314.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11723..12598
                     /locus_tag="SCK20_001236"
     CDS             11723..12598
                     /locus_tag="SCK20_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1145315.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12750..13592)
                     /locus_tag="SCK20_001237"
     CDS             complement(12750..13592)
                     /locus_tag="SCK20_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1145316.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13684..15561)
                     /locus_tag="SCK20_001238"
     CDS             complement(13684..15561)
                     /locus_tag="SCK20_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1145317.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15558..16979)
                     /locus_tag="SCK20_001239"
     CDS             complement(15558..16979)
                     /locus_tag="SCK20_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1145318.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16982..20371)
                     /locus_tag="SCK20_001240"
     CDS             complement(16982..20371)
                     /locus_tag="SCK20_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG1145319.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20628..22019
                     /locus_tag="SCK20_001241"
     CDS             20628..22019
                     /locus_tag="SCK20_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145320.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22092..22739)
                     /locus_tag="SCK20_001242"
     CDS             complement(22092..22739)
                     /locus_tag="SCK20_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1145321.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22747..23433)
                     /locus_tag="SCK20_001243"
     CDS             complement(22747..23433)
                     /locus_tag="SCK20_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145322.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23439..24512)
                     /locus_tag="SCK20_001244"
     CDS             complement(23439..24512)
                     /locus_tag="SCK20_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145323.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24734..25408
                     /locus_tag="SCK20_001245"
     CDS             24734..25408
                     /locus_tag="SCK20_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1145324.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25413..26753
                     /locus_tag="SCK20_001246"
     CDS             25413..26753
                     /locus_tag="SCK20_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1145325.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26902..27888)
                     /locus_tag="SCK20_001247"
     CDS             complement(26902..27888)
                     /locus_tag="SCK20_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG1145326.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8174 a         5274 c         5066 g         9484 t
ORIGIN      
        1 ttttttatat attttagttt acataatgta tattatacaa agtagattta aaaaatgata
       61 aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa tctgcttatg
      121 caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa ttgaatgata
      181 aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga atcattgtat
      241 ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta
      301 ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt
      361 aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca
      421 gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat
      481 acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct
      541 tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca
      601 cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca
      661 gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag
      721 taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc
      781 acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta
      841 ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata
      901 tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga
      961 gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc
     1021 caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat
     1081 cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa
     1141 tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca
     1201 tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata
     1261 ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact
     1321 aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct
     1381 gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata
     1441 actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat
     1501 tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata
     1561 aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa
     1621 taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt
     1681 ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg
     1741 agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat
     1801 acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc
     1861 gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt
     1921 actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt
     1981 aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc
     2041 tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata
     2101 gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc
     2161 aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc
     2221 ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa
     2281 atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc
     2341 ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact
     2401 ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc tatacatata
     2461 attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact
     2521 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2581 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2641 tgattcttcg ttacgcaaaa aaatgtctaa ttcagctagt tccaattgac tccctccttt
     2701 ttgtaagcgg tttctttcgc ttattagttc acgctgtact gctattatat gcaaagctat
     2761 ttttggtttc ataaagactt tctatggttg ggacatgaac ttcctgtgat tccgctattg
     2821 agagaataac cacttcatac tatatttttg ataagcgctt acaaaaatga aaggaacatg
     2881 cttatgaata aaaagatgat gaaattttct ctgctgtcag tctctcttat tttgacttct
     2941 gctggtgcag tcacgtctgc aattcccagt atgtcagcta cttatgccac gcgttcttta
     3001 tctgctgttg aattattaac gactgctccc tcttttatgg tcactatttt tgttttgatt
     3061 agtagtttta ttgctaggaa aatcggacaa aaacaaactg tgatcatcgg attagtgatt
     3121 gcatcgattt gtggtatcct ccccctcttc tcacaaagtt acagtattgt cttagcttca
     3181 agagctggac taggtattgg ctttggattg atcaattctt tggcagtcag catgattgct
     3241 gccttttatc acggagatga acgagcctct atgattggtt ttcaaagtgc atttcaagga
     3301 tttggtgcag ctttaatgac atttgttgca ggacagctag tacagttcgg ttggcaatat
     3361 acctttttag tctacgccat cagcttacct attcttgtct tgtttgtttt gtttgttcct
     3421 acacctgaaa caactgaact tcaatcgcat ggcacagccc caacaagaca agtaatcaaa
     3481 acgaatccta aaatgattag ttatggtatt tttctcttgc tggtcatcgt ggtatataat
     3541 gctgtatcga ttaaattagc cacagtcctg acgacttatt ctctaggaaa cgaaactaat
     3601 gcagcgaata tcttaacaat catgcaattg gcaagtatgg ctgccggagt ttgttttggc
     3661 tggcttcaga caaaagcaca tcgctatatg cttacacttt ctttactact gatgggaatc
     3721 ggctttgtat tgattgccac tgtgataaac ctgtatgtga ttgggtttgg tgcgatccta
     3781 acaggaattt ctttttcgtt attcattcct tacttattca accaagtaag tatccaagcg
     3841 cccaagtacg cggagagttt caatacttca atcttattgg ttggcgctaa tttaggtact
     3901 ttcattgcac catatggttt gctctttctc tcttttgctt ctcttgggag taaaacactt
     3961 gctgttttcg tcaatggcag tatcttgctg ttgattagcg cgcttattag ccttctagta
     4021 gtacacagga cgaatagaaa gacagtgata agagtagatg aagaaataaa agaacaattg
     4081 acaacaaaat aaatttgatg gaggtcgtgt aaaatgacaa atgaaaaaca taaagataga
     4141 tggctttggt atcctggaga ctttgagatc cgccatggac tgctacaaaa tttccaacgg
     4201 gaagaacgag gttttgactg gccagcgtac tggtacatgg atgattgcca tcggaacgtc
     4261 aagtttaaga gatattactt tcttgatcaa ccttccatgt ttaaagtgac gatccaagga
     4321 gttgggtatg tagaaattaa tggacaaaaa catccatgtg gaaaatggct gacttgtcca
     4381 gctggtaaag caaaaattcg tatctttgta ggacatacat cagggctacc agcgatgttt
     4441 atcgagggag acgaagtaaa atcagatatt ggctggacag caagtaattt tattgaagaa
     4501 tatccagctg gctggagtcc gctttatgtc gatatcgcaa aagatccaaa tcaaatctat
     4561 tatcaaaaag aatgcgtcca tccagtcact gaaacggaga ttggcggagg aatcttattc
     4621 gattttgggc gagcagtgaa tggctgtgta gaactaatat ctctggatga ccatatcgaa
     4681 tctatcactc tatgttatgg agagtccgat gtagaagcta tggatatcga ttattgctac
     4741 tataagcaaa taggaatgac aagaaaaatg aaagctcgta aacgcgcttt tcgctatttg
     4801 tttataccag aggtttctac tggaatggtt caacttcaag tttttcatga atttttatct
     4861 ctttcgaaac aagctgcttt ttcaagcgat tcttctttac tgaatcagat ctggtcagta
     4921 tcagaagaag ccttcaagtt atgtagtgat ttgtttttta tcgacggtat caaacgagat
     4981 cgctggattt ggtctggaga tgcgtatcaa tcattcatga tcaatcaata tctttttttt
     5041 gacgaggaaa tcaataaacg aacgatatta gcattaagag gacaaaatga aatcaagcag
     5101 catatgaata ctatcgttga ctattcgatc ttgtggttga ttggtatcga aaaccactat
     5161 atgatgacga aagactatga atttttacgc atcgtctacc ctaagatgca atcattaatg
     5221 agatacttga tggagcaaac gaatgaatta gggtttattt atgggagagc aaaagattgg
     5281 atatttattg attggtctga aatggataaa gaaggaacgc ttgctgcaga acaagtctta
     5341 ttactgaaag cctatcattc catgatcact tgcggagata tcttagctga aacatatgga
     5401 gagaacgaaa attatcatac tcatctctat caagaaaaat atcaactatt gaaacaaaac
     5461 ctatttgact acttttggga tgaagaactt ggcgctttta tcgattgcta tgaatctggg
     5521 agaagaaatg tgacgcgtca tgcgaatatt ttcgctgttt tattcgattt tgctaacaaa
     5581 gaccaacagc aatcgatctt aaagaatgtc ttattgaatg acactatcac acagattact
     5641 actccatatt tcaaattcta tgaacaagat gctttatgca aactcggaca gacgaatatc
     5701 gtctaccaaa cgattcttga ttattgggga gggatgttat ctcatgatgc ggtaactttc
     5761 tgggaagaat acgaccctac tcaaacagga aatgaaaagt acagtatgta tggagatcct
     5821 tatggaaaaa gcttatgcca tgcatgggga gcaagcccaa tctatctttt aggacgctat
     5881 tttatcggtt tgtatccaac aaagccaggt tatgaagaat ttatgataca acctaatcta
     5941 gattcatttg aaacactgaa ttgtacgcta cctatcaaag atggaaagat ttcgcttgaa
     6001 aaaacaaaag ataagatcgt gattacttct gatagaacag gcggaacagt gaaaatgaat
     6061 ggtaaaagtt atcctcttct agcaaaacag ccacttgtct taacaagcta ttcaaaaata
     6121 aatgcataaa acaaaaaact gcttgcgaca tatcagaaga ctttaaatct tcttctgata
     6181 ttgtccaagc aatttttatt ttgaagtttg cttaattctg ttctttagaa acaatgtttg
     6241 ttctttttcc ggcaaaaaga aagctagata atacgacaag taaaaagaaa gcgatgattc
     6301 caactgtcaa ggctatttga ccgtattctc ctagtttccc aagtacaagt cctggtacta
     6361 gataatcagt atcggcaaat gtagaagaag cgattttaag atctcctaat actggaagga
     6421 taaataatgg cagccaacta atcaataagc tattgaaagc agaacctaat accgcccctc
     6481 ttctaccgcc agtcgagttg ccgaagacac cagaagcagc tccgcagaag aaatgtccta
     6541 ctaccccagg aatgataact gtagtttgca agcctatcat agcaaacatg gatagtgttc
     6601 ctacaaagaa gctaacaaaa aagccaatta atacagcatt aggtgcatat ggaaaaacga
     6661 taggaacatc taatgctggt ttagaatcgg gtacgagttt tttagcaatc ccttggaatg
     6721 ctggaacgat ttctgccaac accattcgaa ccccttgaag gacgataaca aacccagctg
     6781 tgaatgttcc agcttggatc agtgcaaaga ttacagcgtt gtctccattg cttagttctc
     6841 tttcaacaaa tccaggacca gctaacaaag caagtgcgac atatacgatc gacatcaaga
     6901 gcatgatcga tacagtagaa tctcgcaaaa agctgaaact tttaggaatc tttatgtcct
     6961 ctgtagaacg gtctttgttt ccaaaaaact ttccgatcca tccactagaa gcataaccga
     7021 tatttccggt atgccctaac gcaacttgat tgtttccagt gatgttcttc ataaacggtt
     7081 gacaaagagc aggcgtaata gttaatagtg ttccttcgaa gaccccgcct aataagatca
     7141 tccacaaatt tgcagtaaac cccgcgctta ctaaaatgac tgctgttaga caagaaacat
     7201 aaagcattgc ttgacctgta agaaaaatga atttatatct tgtgaatcta gcaagtaaaa
     7261 tattcacgat catccctact agcatgatca aagcagtagg tgtaccgtat tgttgcaaag
     7321 caatcgccac tactgcttca ttgctaggaa ctactccttg agtatgcaga gctgtttgga
     7381 acatcgaagc aaaaggggtt agtgaattac tgattaaacc agcaccgccg atcagtacta
     7441 ggaatccgac aaatgttttg atagttcctt ggatcacttc tgaaaatttc ttcttctgaa
     7501 gaacgagacc aagcagtgca atcaaagaaa cgaggatgga gggctgactg acgattgaca
     7561 caaacaaatt caacaattta tccattgata gttcctccct tgaatcattt ttattctttt
     7621 gacaagatct tcgttacttt ttcttttact tcttcgctgt caatgatcga ttgcaaaggt
     7681 acgagctttt catcaggtat agaagttagc gtatctttca aagaatgctc aatgaagaaa
     7741 taatcagcca tatcgctaga tacacttcca acatccgtat gacttacttc aaactcgtcc
     7801 acatctgctc caagatctgc aagtgcttgt ttgatgttca tctctaccat aaaacttgtt
     7861 cctaatccac tttgacatac tgcaaaaatt ttcatatcat tcgtctcctt ttcttatgat
     7921 cgctaatatt tcttcttttg ttgatgcgtg taataaatct tgaagatttt gtttattaga
     7981 aagtagcttt gttaaatttg ccaaagcccg aagatgtgcc tcattatcga tagcagctaa
     8041 acagatgaac aaactaatgg gatgggctgg attatcaagc agatccacag gttgttgaag
     8101 tttcagcaaa gacatcccta cttcgtttac gccatcttct ggacgagcat gaggtaatgc
     8161 gatgtgatcg ccaatatgga taaacgcacc atactgctcc acgcgctcga tcatagcttt
     8221 gatataactt tcttctattt tattttctga taatagcggc tgtgcggcaa atgtgattgc
     8281 ttcttgccag ttttttggtt gatccgataa ttggatcatt tcttctgtta ataattcaga
     8341 tagcatagga cgttgatcct ccttcttttc taaaagtttg ttcattttac gatttaatac
     8401 ttgatagagc ttttcttcgt ctacgccttc ttttaattcg atataaggca gtagcgccga
     8461 caacaattct tgggcagaag gcattgaaaa tcctggtatc agccaatctc tttgtacttg
     8521 attgatcagc tgattttttt ccaagtcagt catgattgga tgcagaacat agagagactt
     8581 tttcttatca gtaagtgtga gaggtacagt agaaaaaata atatcgtaag aagtttcgtc
     8641 aacctcgtgt aagcggtcag ctgaattagt ctctgtaaaa aagatcatcg ggaataattg
     8701 ctgaagttca ctttttaaga tcaatgaaga gctaatgcca cttggacaga cgatcacggc
     8761 tcttattttt ctatttcttt cttctgcgtc cgcttttctg atctctccgc caaatagtat
     8821 agtgaaaaaa ccaatctctt cttcagggat gctcttccct attcgtcttt ctaacggaag
     8881 taatgctttt cttgtcattt cgtagatgga agcatattga tgcttaattt gatcgatcat
     8941 cacattaggt aaataaaaag aataggaaat acgaaaatag gcagggacca agtgataaaa
     9001 gacattcatc aataattcac gaaacgaagc aaattctacg gccatcagga attccatgcg
     9061 atggatgact tctgaagaac attccagcaa aaaatcaagt gcaggctcct ggacatctcc
     9121 ttcaacgatc gatgctaagc aaagagagaa aaatataatc tccgaatttt gatgttcgga
     9181 atagccaagg ccagtcaata cttgtcgact aatgtcagtg actgcaagag aagccaacag
     9241 ttcagcatct tttaccagtt ccatggatgt ttttccaatt cgaatggttg tcaaactcaa
     9301 ataataagct aattcatcca ttcgatttgg tgcgaatcga agatctgata gtgaaatcgt
     9361 tttatctagc aaatctctaa ctttggcgta ataatcaatg gaaagggttg cggttaaata
     9421 cgtgagccca aatcttccag caagtgactg catcaaacga gcagtgcgat tttttgcttc
     9481 tcttcttaat attgcttctg accctagcag cctaaaacca gttttcctag tatacgacag
     9541 ctgacaatct tccacttcta attcatttct tatctttttg atatctgaca ggactgtccc
     9601 tttagaaacc tgaagaaaat cttgaaaatg aaaaacagaa acagacgttg cagtcgaata
     9661 aaccatcaaa taaagaagat ctcttcgctc ttgttcggta tacacagctt caaatcgagt
     9721 aatatccaaa aatttttctg cccagtcgat tgctcggaat ttctttatac ggatacgggt
     9781 atcatccaca tattcggcaa aaggagttaa ttgtttgatc gcctcgcttg tatttacttc
     9841 gatgatctct gctgcttttt ctagactgat ttctcttggt gttttcagga gataactaat
     9901 tttgtagttc acagcgctgt tcacctccta cttatattct agtatggtag cgcttttaaa
     9961 tcgaggggtg tttggtacaa cttacagttg gatttttttt acgactgtac cgaacgattt
    10021 ctatcgaatg gatacagaaa aaaagcaggt gaccaaatgt ctcctgcttt tatgaattga
    10081 tttcgtccga tcaatgatga tcatgtttat tttttgaggt cagttcttca aatttttctt
    10141 tgacatcttc aaccgctttt tcgatatctt tttcaaagtg attgggctca ttaggctttg
    10201 tttcctcttc tacagaattt gaaccagata aagctgtttc tttattttct gtttcaccag
    10261 agtttgtgcc agctaatgat tcttctttgt cttctgtttc accagaatta gttcctgcta
    10321 aagccacttc ttcttctgtt ttaggggcca ttgaacctct taatcgtttg tgtttttcac
    10381 cgtcataatt tgtcatgttg gttcctccta gtagttctat actttttcat cgtacaataa
    10441 gacagtttat gcgtcaaaaa acaaacccga ttcttacaaa tatgtcatat ttttccttct
    10501 gattcttgaa tgcttttgat tccttcttcc aaaattttca tttgtaagat tgtttcttca
    10561 tctttgatga ctttttctgc gccattaact aatgtctcgt aaatcccttc gtagacacgg
    10621 ctgtaatcac caacctcgga aattactttt tcttcatgat aactcccttg ttcatctata
    10681 taggtcaata caccataatg ttcgggcaaa tcgataccaa agtcttcatg atcaggcatg
    10741 taaaacattt tcagatgttc ttcttgtcga tcttttgttt gtttcacgaa catccctttt
    10801 tttccgtaaa cgacgaaact tggacgttct ttgatgcgga aataactgga ttttatcgaa
    10861 attttcatat ttccatagaa aaaatccaga tcgaagtaat cattcattcg tcctttccct
    10921 aaaagttgac gtacatcata gctgatatca tctggttcgc cgaaataaga aagaacttga
    10981 tctactgtat gacaagcatg tccatataaa tagctgttcg cagtagaaaa ttcatgcgta
    11041 ttttctggta cttccggtct gaaataatca taatgcattt ctacttctaa aatatcccct
    11101 aattttccgc tttcgattac tttttggact gtcaagaagt cagaatcgaa ccgtctgttt
    11161 tggtaacatt ggataaatag acctttttct tttgctaatg caaataactc ttctgcttcc
    11221 tttgacgttt ctgcaaaagg tttctctact agtacattct taccatgttc gagtgccatt
    11281 tttccatatt cataatgaaa ttgactaggt gtagtgatga caactaattg gatctcagga
    11341 tcatcccaaa gatcagacag ctgatcagta tattcgatgt cttcgaatgt tttccacact
    11401 ggttcagatg gggaaaaaat ttttttgata ttgattttgt tcgtaagttt tcttgaaaaa
    11461 ggcaaatgat agcgatttgt actctttcca ttgccaagat aagcgattgt aagcatgagt
    11521 ggtgcctcct tttattgatg ttttcagtat atcctagaaa agcgcagaaa gtatttataa
    11581 tagctattct ggtaaaaaag ttcagaaaaa cacttgttat cattcggttt ggtgaaatta
    11641 atgttcaaag taaggataaa aatcaaaaag atagtagcgg aaccaagtgt ttatcagtaa
    11701 gatagaattt ggaggtcaga tcatgcttat tgaagaaaaa ctaacaaaac aagagttgtt
    11761 taccacgaca gagaaacgga ttgtcgatta catccgtagg aatatcgaag ctgcagtcta
    11821 catgacgatt gaagaattag caaaagctac gtatacttcc cattcagcta tcattcgttt
    11881 atgcaaaaaa atgggattca gcggatttaa agaattccgt ttcgagctgg ggcgggaagt
    11941 tcatcaattg attgctcagt tcaatcaaac gattgatcct aattttcctt tttcaagtga
    12001 agatagcact cagcaaattg caaagaaaat ggcggattta tcgatccagt caataaaaaa
    12061 agcacaattg cagattgaac atgaagattt agatcaaatc ggtaaagtat tgaacaaggc
    12121 acagcgaatc tttctttttg caaaaggtga ttctcaaatc acggcaagga aattccagaa
    12181 taaaatggtc aagttgaata agtttttgat catggctgaa gaatacagtg attcttcttg
    12241 gaatgcagcc aatttgacta gcgaggattg cgccattttt attagctaca gcggtcgcat
    12301 ccatcattat gaacgaataa tgacctattt gaagcatgta gaggccccta ccttgctgat
    12361 taccggaaat cagcattcag aaatggccaa acaggctgat atgtgtcttg ttatatcgca
    12421 ggaggaatat gattttgcta aagtagcgac tttctcctct caaattgcct tcgattacgt
    12481 attaaatacg ttattctcag tgatatattc tcaaaatttt gaagagaata tccgcaactt
    12541 gaaaaacaaa caagaattga tgcaaaaagg ccttcttaaa gaatatcgga aaacttaaga
    12601 aaagatcctc atttagtaat aagaaagata gtgtttgata ggctctcttt gatatcatca
    12661 taaaaacagg ctgaagtttt ctttttcaaa cctcagcctg tttgtttacg gaacgattcg
    12721 aatagatata ttatagaaat gaggaatagc taacttctgt ttcttatttt gaacgattga
    12781 cgaaaaagat atataagaag gaggaaaatc agtatacatg cagctaacaa tccatacagg
    12841 aaaaatcgtt tgttctcgga tactttctgt acttcttttt gaacttcttt tgcatgataa
    12901 ggaacccggt gccctctgac aagtaagcga tgactgttga ccatataagg tgtacaagtt
    12961 aggagtgtca cgaaatcctg ccctttttcg attctcagat cttttgtatc agtcggttct
    13021 accgtcttga tctgatcgac ttgataagcg agtgtccgtc cattgatttc gatatagaaa
    13081 gaatcaccta aagtcagttt cggtaagtct gtgaacaact ttgcttgagg cagtcctcga
    13141 tggctggaaa taacggcatg tgtattttct ccccccgtag gataagacgt tccttccaat
    13201 aatgaggcac ctttttctag cagcacagta ttcgtttcat caaaaattgg caaatggaca
    13261 ttgatctcag ggatcgtcaa aataccgatc gtatgtttct caaaataggt tctatccttt
    13321 ttcgcaggtt cttttttctt agtaaaggga tctgcacctg gatttccacc ttcttttgct
    13381 aattgctggt ttttctttgt catgttttct tggattttag ccatgacttc ttcattttct
    13441 ttgaccgctt gttgctgata atgagagatg atctgctgat ccagataatt attcagcgcg
    13501 tcgctaacaa atggataagc tagtatccct attcctgaga gaagcaagaa aagcatcagc
    13561 agatctagaa gtttcctttt ctttggtttc atttgtgttc tccttgtgat gactgtttga
    13621 taataaaaga aaaaggagca tttgcgtaat ccgctgctcc tttttccaat tcttgcaaaa
    13681 agactaaatc tgactgtgct tgcgtctagc aaagtacagt ccagcaatca gtagaaggac
    13741 tactcctgca ccgatataca catagattcc cttaccgcct gttgaaggaa gtgtaccttt
    13801 gtgtttattt ggtatttttt caggagaaat caactgtcct gctgttacat aagattgttc
    13861 atcgatagta aatgctacac ggtttgttaa tggaacatat ttttctggcg ctttcgtttc
    13921 ttccagatag tacgtgccat attttagacc tgtcacatcg attaaaccgt tacttgtggt
    13981 tgtaaaaacc gttgctgatt ctttcgccga tacccagctg acggcttttg tggatgggtc
    14041 gatcgataaa tatttcgctg tgtcactatc ttgatcacga acgacgaatt ctgctccagc
    14101 aagtgtttgg tctgatgtaa cgtcaccatc tactttaaca aatcgtttgc ccccagtaac
    14161 gacatcgact gacggtggtg tttgatcatt tgtatgcccg ttatcgacat ttgcttggtt
    14221 agaaaatcct ttggttggat ctgctttttc gttcaaatgc atatagtaaa cgaatttcaa
    14281 tgtaccgcca ggagttaatg aaggaatata attcggatca actgaaaccg tgaatccgtc
    14341 tgtttgctca gtgacagaat aatttactgg gtcgatttct ttatttccat catataaggc
    14401 ataagcatac gttcctgaag aatcattatc aaatgttaaa gcagcgtcat gagtatcgat
    14461 aagtttgaat gttgtgtact tgttttgcgt tccttcttta tcagcgatac ctaatgggat
    14521 attgacagaa atctcatatt gaattttttc accgattgcg acatcttttc cattcaattg
    14581 aggtgttgtt ttatcaactt tagacgtatc atttttgatg gtcttttcta ctggtgtttg
    14641 gctatttgcc aggatctcaa aaggcgtgat tgtttgcttt tcaatcattt ccgcattatc
    14701 aggagctttt acttcttcta aattatattt tccaacttct aaatgattaa cgatcaactg
    14761 gcctttttca atagatgcct cggcaaagtc attgttgccg atgtcataag aatgaccagt
    14821 aatgaaatgt ttggctttgg tttgatcagt tgtccaagtg tacaatccat ctgtgacact
    14881 ttggatgtat tttttgacgc ttggtgttcc ttcttcttta gaaataataa attctgctcc
    14941 atttagtgct tcgttttcgg cagtaccgat ttttgtaact ttcaacgttc catcattacc
    15001 gactgtattt ttagggtaga gatggatagt atctagttct tctgtcccgt atttataaga
    15061 gccatctgct tgtttgatca tctcatatac agggaaagct aaaaccatgt ttgcggcagc
    15121 tgacactccg tcttttggtt cttctttgat cgtgtagact gcatctttcc cattttgttt
    15181 tttaggtaaa gataaagtga catttccatc agcatctgtc gttcctgaag caactggtgt
    15241 accaggagtc aaagactgga ctgcttgttt tgctgcatcc acggacgctc ctttatctcg
    15301 ttgcgcataa aattcttgag tgacgttata aactgaaaat gaaatatcgg ctaatccttg
    15361 gtattgatcg aattcgctca tttctttccc gctgttttgg attaaaggat cgggtaaatc
    15421 agtcattttc tttttatgaa ggatgacttg agctgctgtc tcctctgcaa acactttttt
    15481 tgcgccgaag ctgtttgtga gtaatggtaa aataaggaaa aggactccta acataacgtt
    15541 tattttttta tgatttttca ttttttcact cctgttcttt ttttgataaa caaccagatt
    15601 gcaaagcttg cgaataccat accaacaacg cctaaaagtg tatttttcgt ttcgtttgtt
    15661 tttggaagaa atggacggct attttctttt ttattgacta ctttcaatat actgtccgcc
    15721 tctttatcct cagaaagaaa agttgaaatg gtaaacggaa tttctgtttc acttaaaata
    15781 taaccagaag gtgcttcgat ctcttttaat cgataacttc ctgttttcaa cccggaaatt
    15841 gaaaaagcgc catttttatc ggaaatcagg tgaagcgcat ctgattcgtt ctttgtccac
    15901 ttgtatccgt tggctgtttg ctggaggtaa tttcctgctt tgtttttaat aaggaatact
    15961 gcgccagcta atcgtttatc tggtttattt gtctcgattt tgacaaattg cttactccct
    16021 gtatatacag agactttctt tgtactgaca ccaaaaccaa aatccagtgt tccgttgttg
    16081 ttgatttcct tgttcgcctg tgctgtactg cttagctgca tctgataaga aatggtcagt
    16141 ttttttccag catatttttc caacgaaggg atcgaaaata aaacatcaaa tccgtgaggt
    16201 ttcttgtgta tgacaaagcc ttctgtcagc ttttctccat tcgatgaaat cgttaaacta
    16261 ttaggtaaaa acgttaatgc ttcatccgca ctatctgaca attcgaactt ttgatagtca
    16321 aggatattta caggaattgt cgtatgcaag gaataagtga ttttttcacc aatcgtaaaa
    16381 tcatttctag gctcttctaa taccttctca aaaggtggat cagggtagtc gttttcttca
    16441 tttttaggat acaaatggat agttgaaagt ttttgattgt tttgtccatg tacaggaaga
    16501 acaactacca tattctctgc cttttctttg acgacttctg gtactttgga ttcaatgaaa
    16561 agatacgctt tatctctttt ttcttgatct ttagcggcca gagaaaaaga agcgatacca
    16621 tcttcgttgt taagagttgt agttgttgct tctgcggtaa acataccgga agacgcaccg
    16681 ttttttgcga tctctgcttg tgcttcttct accgttttgc ccttttcccg tagatggtaa
    16741 aaagaatctg tgacatcata aacttggaat gtgacaccat ttaatcctcg atacgtttgt
    16801 aataaagctt tttcttgtcc gtcatttgga tgatttttcg gcagttgccc gtttgggaaa
    16861 agcaatttgt gtaaagtaat ctgaaccatt tctgtttctg tttctgttgc tacctgagta
    16921 aaagctggtt taaatagtaa caagaagaga cacatactaa gccaaccaag ttttttcata
    16981 tttagctgcc ccctctttct tttttcaaaa aaagatacca aatagaaaac gcacaaccaa
    17041 tcatccctac tagatagatt cctaaacggc caattcctcc cgtttcaggt aatggtactt
    17101 ttgcctgatt cgtgatgtct aaagaaatct ggttgttttt ggctcctggt gacagaacag
    17161 attcatgatc ttgtccatcc acttgaattg acccatcttc gtgtataact atagtaactg
    17221 gctcttttag accttggtat ccttctggtg taaaagtttc ggtcagcgta taagttccag
    17281 gttttaaatt ttcgaataga aaggtatctg tttcttgtcc atcttttggc gattctaagt
    17341 ccatttctgg tccctgcagt ctgaattttg ctccttttaa tgtctgtccg ttgtcttctt
    17401 ttttgtgtac cgttagatca aacgctttta gtcgattttg atgagtgagc gtccatacat
    17461 tatttgtcat ctcggtattt tttcccgtgt aaatgatttt tccatattta tcgatttgga
    17521 attcataatt tcccggcgaa gtatcatatc cggcaggacc agctgtttca acgactcgat
    17581 atttaccagg tgtgagtgaa tcaaaactgg caagaccttt ttcattcgtt gtttggctgt
    17641 caattggctg ataagtacca tttgcttctt ttttctgtag ggaaaatgct gctcctttaa
    17701 gatttatctc tttgtccgtc ccttgcatcg catacttacg tactgctaca ggaacttcaa
    17761 caaaaggatt ttcaatagtc aactggatcg tatcatcgga agtagtgact gtttttccat
    17821 caatggttac cgtaccatca gaagcgatct tgatttccca agtagtcttt ttgcttaatc
    17881 catgcccttc tggagctttt gtttctgtca gcgtataggt catttctttt tgcaatttga
    17941 cattttctgg aagagaatag gttccgtcgc catgatctgt tagtaaagta tcaatagaat
    18001 ctcctgttaa tttgaaaaca gcgccaataa gatccttttc acctgtagag gaatttttcg
    18061 ttattttcaa gtttaacggt gtgaattgtt tagtattttt ccatgtcatt gcatctattt
    18121 gttgagaatc gtatcctggt acaggtaatt ctttgattgt ttggtaactg aatgcttgac
    18181 cttgattatt gtattgaggt agtgataata tctcttgata actttctccg ctatttgcag
    18241 ataatttgtc aatgtctgca cgttcccacg tatttgttgt atctttagct ggtttaatga
    18301 ttcgaatata tccgtttttc caagctgcag catttgtcgt atgttcccgt tgaatctcaa
    18361 aagtaacttg atctggacga tcagctagat tgttgtcaaa ttcttcccat aactttttga
    18421 tgtgaagact gactccggga gctttagcag atggtatacc gaattcagct aattcattgg
    18481 tatcaatgtt tggctggaaa gttgtcctgc cgttcatttg ataccagaaa tttggatgga
    18541 agtcctcatt ttctgtttgg attctcactt ggtaatggat ttggatttct tggtcttttc
    18601 ctaaatagat ctgattgctc ttgatggtat ttccttctat ggaaatagat ggagtgaccg
    18661 ttgtaggact tgtccccaca ctcttgactg aaagtgtacc aggctgataa atgaatggtt
    18721 ctgcgattgg atcatttatt tgtccattgc ttacagttgc tgagatctgt acagcttttt
    18781 tggctaggta ttcagagata tctgttgcat gatcagctga ttcatagtaa agatcgcctt
    18841 tttcatctga tgaaaccatt tgtcgcatac gagactctac ttctgctttt gagagaccag
    18901 ctgccggatc gctttgaagt tggatgccaa gaccatgtat ttcgattcct cgttctttga
    18961 gtgccatcgc ttctccgatg gttgcgataa acgtactgtc gattcttctg gatagattgt
    19021 tttggtcagg tgcatcatag attcttgaga ttagagaagt atttcccggc cgatcttgcg
    19081 tattagaaaa ctgagttccg taataattgc tgcttgattg tgcgtgtacc cgctgtactt
    19141 tataggaaaa tgttggtaca ccatccgtca gcaaaacgat cactttttta tgtccatttg
    19201 gaacggatag catgcttcct gcatctctta gtgctttttg tgtaaaagta ccaccatttg
    19261 tccgtgaagg tgtaatggat tttacttgat ttttcactga atcaaatgaa cccatctgta
    19321 ctgcaccgtt actgtagcta tatccttcgc ttgagtagcc gacatatccc atattgattt
    19381 tgtctgtgat accgctatct gctaaagtat cgacaaaacg atcgacacca atctttactt
    19441 caccgatccg attattgtcg ttcatacttc ctgaccagtc tacgaccaat acgagatcaa
    19501 gaggcgtgat atctttttgt acatttccac gagcattcaa ataaacatca aacaatccgg
    19561 gtgtacttgt ttcctttgca tactttcgta tcgcatagtc tgcttgatta ccggttcctc
    19621 catattcgat atacgaatgg gtccgatcat taggatctcc attccaagaa tttataccat
    19681 cccattgatt tgttccgttt ttattgcctt gatgatcaag aacatttgta tttcctgtag
    19741 gttgccaact ggctttagga taagtgccct gatcatctgt tgtatattct gtaggaatcg
    19801 tttgattaga tggcacactc acagctggca aggataatgt aggcaatgta aatgggctga
    19861 cagaattttc gttttctttt gatgattctt cttctatttc gtctggcttt ggtactgaca
    19921 catctgtatc tttcttattt tctgtctcat caataatgtc atattcttta ttatcgacga
    19981 tcagcttgtt tgcctggctg tggtaaagtg ctggatcgat tggaaaactc agatcgatag
    20041 tttgtttagt gtttgatgag atttccaacg taattttccc gctatttacc tgataagcat
    20101 agccatctcc ttcttttctt tccacatcac ttaagtaatt ttcgactaga ccaatttctt
    20161 tttctatctt ttcagcactg gtgttattcg catttagctt gatgttcagt gctttttttc
    20221 cagactgatc cgaaatgact tcagctgaaa ttgtttctaa aactgttgga tcatctgtag
    20281 tgtctgctat tgcagataat ggcacaaagt ttgataaact caatattatc agcatgaaaa
    20341 taactttcag tttcttccct gtggtcgtca tttttcccct cctttttctt tttttctgtt
    20401 ttgacgtgaa tagtgaaaat tattaaaata aacaggcgtt acataatttt atttatttta
    20461 atatgataat tattatacga agggcgctaa agggaatcac accaattttc ataccaattg
    20521 gtttcattta tgaagttgat tccttcgatt aatgagcgtt tcattgaaag aaaagggttg
    20581 agggagaaaa acatatgatc aggttgttgg aaggaaacta tagaaagatg gaaaaaatca
    20641 ttcgaattct gtccatttct gacaagtgga tgactaaaaa agaacttgcc aaacgaattg
    20701 gtagttcaga atctacattt gtaaggtata tcgaagagat caaaatccgc tggggaacgg
    20761 atgtaacgat tcaatcttca caaaaattgg ggtatcgtct tgaacatttg aatgtaccag
    20821 cttatttaaa tgttctgatg gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg
    20881 agattattgt caatccgggt aaaacgattg agtattattg tgaaaaaatt tccatcagcc
    20941 ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc tatttattaa
    21001 aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa aagtgagctc cagttgcgta
    21061 tctttgttac ctttttcttt ttaagttact acggaagacg cacgttgcca tttccaatcg
    21121 aaaaaaagaa gatcaaagaa attatggtca agaataattg tcagatgaat ttgtttcagg
    21181 aagaaagccc atatgagcaa tctttcttta ttatgtattt tgttgtgcat ctcatgagag
    21241 aatcccaagg aactccattt gaatggaaag ctggagactc ttatgtacag ttgataaata
    21301 tcgataaaaa agacttctta atcttgaaaa atctcttagg aaaattgaag tacagcagct
    21361 atataaaagt agtgaactat tttgtcaata cgattttttc agaattttcg gagaaagaaa
    21421 aaaatgaaat cagtgagcag atacgaaaaa atatgtccta ctgttcgttt tcatccgctg
    21481 attgcaaaga aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt tattttttct
    21541 cccgtatttt cccatacgat acgactcatt tgacgatccg tgcaacttac tttgcacagc
    21601 agatgcaaca ccgccagata cgtctatatg ctggatttcg tgcagaactt caattttatt
    21661 ccactctact gaatcagaat ctgatttatt atttatctag ctatatcttt tggatgctta
    21721 tcggtaatcc tgaaattcac cattatactt ctgataaaaa aatactgatt atcagcgact
    21781 taagtcttcg acatagtcaa tatattgaag aatatatcag tgatattctt gctgtgcata
    21841 aaattcactc tgagacaact gctattactg aagatcagct gtcgaaatac aatcttctcg
    21901 agtatgatct gatcgttacg aatcaaccca ttttgaatgc ccatgttcct catatattga
    21961 ttgatgatag tgtgtcattc gcaaatgaag aagaactcat ccgcttattc gagttatgaa
    22021 taatcaaaaa acagctatgt ataaaccacc ctgataaaaa tttcaggaca aatacacata
    22081 gctgtttctt tttattgtat tccttcagca atccaacgag atttatagcg aaagtctttt
    22141 aacaactctt cttttgtcca attatttgca atgagatccg tcaaagtgtt cattacgagg
    22201 gtcatggaaa agtataggaa actaactgct tcttccgacg attgaatgga gaaatgattg
    22261 attcttaata attccagcca tttttcgttc attggagaat cagccgatag ttctctacgt
    22321 ttggttgtct gctgatcatt tcgctgggtt aacaagtata acctttgcca atagatactt
    22381 tcgtgatcta agtcgcagca ccactctaaa tatttttcga tccccaaaaa gaaatgttgt
    22441 ttgttttcgc taatgaattt taagatatct tgatggacta tgtgagaact gcttttgatc
    22501 gtatacaaat aagcatcttc caaatcttga aaatatttat aaaaagcacc gcgcgacatc
    22561 cccatcttgt caacgatttc tgagactttg acttggctga tgtgctgtga gcaaaatatc
    22621 tccagtaata ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa aaaagtttct
    22681 tttggcatcc acatctctcc tttatctctg attatacgtg gatagccctt cattgtcatg
    22741 aaggattcat tccttctttt tctcttcttc tgtcaagtac ccatcttcca ttcgataaac
    22801 gcgatcactc cattgaatca tacggacatc atgagtaacc ataatagtcg ctttttgttt
    22861 ctcatgagct tctttaacta gtaatttgac gacttcatat gcatgatctg tatccaaact
    22921 agctgttggt tcgtctgcta agatcaagct tggctcatta tataaagcgc gagcaattgc
    22981 cactctctgt ctttctccac cagaaaggtc tttaggataa ctatttttaa gtgactggat
    23041 atctaaagat tgcagcaagg aatcgatttt tgttgaatct tctttttttg aagtaacttg
    23101 gtcaactagt tcaaattgtt cctttaccgt taaaaaagga atcaaattag agctttggag
    23161 aataaatcct atttctttaa agcgtaaatc tgctcgtttt ttttctggaa gctcagttag
    23221 tgagtgaccg ttgatcacta tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt
    23281 aaggaaagta cttttacctg aaccagaagg accaatgatg ctgacgaatt ctccttcctt
    23341 tacataaaaa tcaattgctt tcaaagctga gacctttgta tgaccagaac caaatgtttt
    23401 ggtaactttt ttcatcgtta atattttttt catttttatt aacctcctat tgctgtaatt
    23461 gggtctactt tggttatcgt acgtatcgaa aacaatccgc ctagcattgc gacaatgatc
    23521 aaaatcccac tatatagcaa ccattgtgac caaaaaacag caaaaggcat cgcgcttggt
    23581 aaaatcagac tagtcaaata agcaaacaaa agagcaagca gaacgcctac caccccaaca
    23641 ataaaggact gtgctactaa agatttagcg atgaagaaat ttttgatccc ttgtgctttc
    23701 attactccaa aaatagcagt cttctgaagt gtcatcacgt acatgaaaat accgaccacg
    23761 gcagcaacca ctaagaacaa aaagtatatc atcgcattta aggtcatatt ttgggcagaa
    23821 tagccaggaa tactttcaat gaatgtccca atagttaata actgtagacc cttcgatgct
    23881 ttttctttcg aaattttagc tgaatccttt attacgatcc cattgatcgg ctgttcttct
    23941 tcagaagcaa acggctgttg tccatacttc aaagcagtcc acgtatctag ggacgcatag
    24001 atgacgggag aaactgtgta ataagtttca ggaaagatac cgacaatcgt caaaggatcg
    24061 tcgtaactgc caattttgat cgtatctcca attttatagc ctgaatcagc cagattttga
    24121 gagatgatga tttcattctt tgttttaaat tcatgccctt tcgtgagttt tggtaataaa
    24181 aatgcttctt tcgttgtgcc gaaaactgta acattttctt tgttttttcc tttgattgct
    24241 ccgctgtaca atccgattgg tgctttctct cctccttcaa tgtatttcag atctcctctt
    24301 gttaattgtg aagctgcaaa tacttgattt gcatcctccg acaaaacaat ctcttgtgcg
    24361 tcccaatcat cgattgcttt tttaaactct tctgaaagtc cattcgctag tcctgacaac
    24421 atgaatacga catacgcaat caagaacata ataccgacga tcaagccata tcgtaattta
    24481 gaatagcgaa tttctttgat agctagaaac atttgttttt cctccctatg atagattgat
    24541 agtgacaaaa tgtcacttgt tgaatagtgt aactctaaag tgacaatttg tcacttgtgt
    24601 tgtttttttc ttatcaataa attgtctaag acatctttcg ataaaacctt aacatgagtc
    24661 ataaaaaatt tatttaagcc ttatttatgc tttccatgat aaactaaagt gaccaaatca
    24721 atgaggtggg aaaatgatta aactattagt agtagaagac gataaagtat tatccaataa
    24781 tataaaagaa atactcgctg caatcggcga gataacacaa gtttatgatg gtagtgaagg
    24841 actgttcgag ttggaaagtg gagtttatga tcttgcaatc ttggatttga tgatgccgga
    24901 aatgaatgga tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga ctccagtatt
    24961 gattttgact gcaaaagata gtttggaaga taagattgaa ggatttgaac gaggtgcaga
    25021 tgattatcta acaaaacctt tctatcgaga agaactgatc atgcgcgtga aagcattatt
    25081 gaaacgctcg ctaggtctct ttgatgaaaa tgttctgaag tataaggata tcagttgtga
    25141 tttaaaaagc aatgaagtct tgtataaaga acaagttttg cccatacaag gaaaagagtt
    25201 tgaattgctc gtttattttt tacaaaataa gggaatcatt ttgacaaaag agcagatatt
    25261 tgaccggatc tggggatttg attctgatac gacgattaca gttattgaag tttatatgag
    25321 caacttacga aaacatctgc gaagtactgg attggataaa gaaatcaaaa ccttacgtaa
    25381 tgtcggttat atactgcaaa gggtgtgatg caatgaatgg agatattaca cgaaagcaac
    25441 gtagtcgctt ttttgcgaca aatgtagcag catttgcttt tatctttttt cttttgggat
    25501 tcattacttt acagatatta aaccaatctg catacagaga aacagatctg tcattagaga
    25561 aaataaattt tgattctcga acagtacaat tagagataca aagatatcag caaggcgatc
    25621 cctttttaaa cccgactcaa acggaacaaa acagaacaaa catggaacag ccattaatgg
    25681 aaccatcaaa tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat ggtgagatat
    25741 tgaataaagc agctattggt gggcgtttca accaaatcaa agaattaaaa atggatacga
    25801 aaaatctgaa taccatccaa aatattgaat taaatgaaga cagtggctca tctgccctct
    25861 ctttccgttc gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa
    25921 ttttagaaaa tacaaatcaa atcaagaatt cattagcgac ttttcgcacg attatgattt
    25981 tgtgtatgat tgttttttgg attctctcta ttggtatcag ttattacttg tctagtctaa
    26041 gcatgcgccc tattattctt tcttggagaa gacaaaaaga atttgttgag aatgcttctc
    26101 atgaattgcg cacaccgtta acgattattc aaaatagttt ggaacatctt tttaccaagc
    26161 ctaatcatac tattattgaa gaatccgaaa gcatcgctca agcattgtca gagacgaggc
    26221 gcctcactgg attgacttcg gatttgctga caatcgctcg gaatgattct gatcagcagc
    26281 tgctttcaaa gcaaatgatc aatacacaag aatatatcga acaacttgtt aaaccatttc
    26341 aagagatggc catcatagat ggaaaagagt ttattctcca taacgatgca acattagaag
    26401 taccaattga tgaaaaaaaa atccatcaag ttttggttat tttgctggat aatgcattaa
    26461 aatatacgaa tccagaagat aagatcattt tggattcatc tgttaaccat aaagaatggc
    26521 tgattcacgt aaagaatact ggcccttcta tcagtgaaga agatcaagca aggatttttg
    26581 atcgatttta tcgggaagat caatctcggg cgaaagagac tggcggatac gggcttggct
    26641 tggcaattgc aaaacaggta atcgaacagc atcatggaca gatttctgta tccaattatc
    26701 ttccacaagg agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat
    26761 ccctaaaaaa gggctgtgac aaaaatcttg tcacagccct tttttgttgc gaataaacgg
    26821 tgttccagaa gtagcttctt cggaaataag ccgaaatttc tttaagctga ccacttctgt
    26881 cacaacctct ttagcttacg cttacgattt tttattgcgg tcttcaatgt gtacatcacc
    26941 gcttgtgtca atatccaatt cttctcggct aacatcttct gtcacttttt caacatcttc
    27001 atgttcttct ttatgaatcc caacttcttc acgaataact gtatgtttgc ttacatctac
    27061 ttgctcttct ttgattggga tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg
    27121 tgaagattga tctgttactg gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac
    27181 ttctaccgtt tcagtatcgt tgaccacatg tttgtggatg tcgacttctc cagttgtcac
    27241 atcatgtgta tttacatcta atttttcttc ttttagttga attgtatcat catctagatt
    27301 tggtttgcga tcgatgtctt cagtcttgct tgtacccatt gtatcagtct cgccaggtac
    27361 gtccatcgga tcagttcctg gtgtcatatt caatggatcc gttgtatttt gaaagcctgt
    27421 cgtgccacct acgtccattg gatctgttcc agtgccagtt tctaatccag tccccattgt
    27481 gcttgctggg ctaacagtat cttgtgtatc cattgtatct tctgttgttt ctggctgacg
    27541 atagcctttt actgtgatga caaattttcc atcagaaata tctcctctat attgatataa
    27601 aggatcattt tctggtgtag ccgtctcaga atcataggta tcaaaagaaa aagtatcttt
    27661 tattttttcc cataaagaac ggtcttcttc accatccatc cgattttcat ctgtttcaac
    27721 atctatacca gacaatccgt caaatcctag tctagatgct gcttcatcag tagtataaat
    27781 aacgatatca tctcgttcat acccttcgtt acgcaaccgt tcaattactg ataatgcttc
    27841 ttccctcgta ctatagcttc cgatgaccgt taaatttttc tcttccatgt aaattcctcc
    27901 tagcaaatag atttatgatt ggtatattgt tacttataaa tttattctat aggagacgat
    27961 tgttttctac aaatatttag attggaattt gattaaaa
//
LOCUS       DARBEG010000029        26605 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000029 DARBEG010000000
VERSION     DARBEG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.029"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1152
                     /locus_tag="SCK20_001248"
     CDS             <1..1152
                     /locus_tag="SCK20_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEG1145327.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="SCK20_001249"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="SCK20_001249"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG1145328.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="SCK20_001250"
     CDS             2728..3003
                     /locus_tag="SCK20_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG1145329.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="SCK20_001251"
     CDS             3009..3776
                     /locus_tag="SCK20_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG1145330.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="SCK20_001252"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="SCK20_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG1145331.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="SCK20_001253"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="SCK20_001253"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG1145332.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="SCK20_001254"
     CDS             7920..8222
                     /locus_tag="SCK20_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG1145333.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="SCK20_001255"
     CDS             8225..9388
                     /locus_tag="SCK20_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1145334.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="SCK20_001256"
     CDS             9414..12842
                     /locus_tag="SCK20_001256"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG1145335.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="SCK20_001257"
     CDS             12863..13987
                     /locus_tag="SCK20_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145336.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="SCK20_001258"
     CDS             13977..14414
                     /locus_tag="SCK20_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1145337.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="SCK20_001259"
     CDS             14398..14736
                     /locus_tag="SCK20_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG1145338.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="SCK20_001260"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="SCK20_001260"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG1145339.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="SCK20_001261"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="SCK20_001261"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG1145340.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="SCK20_001262"
     CDS             15893..16936
                     /locus_tag="SCK20_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG1145341.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="SCK20_001263"
     CDS             17095..17823
                     /locus_tag="SCK20_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG1145342.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="SCK20_001264"
     CDS             17939..18184
                     /locus_tag="SCK20_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1145343.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="SCK20_001265"
     CDS             18181..18399
                     /locus_tag="SCK20_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG1145344.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="SCK20_001266"
     CDS             18535..19074
                     /locus_tag="SCK20_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG1145345.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="SCK20_001267"
     CDS             19067..21340
                     /locus_tag="SCK20_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG1145346.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="SCK20_001268"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="SCK20_001268"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG1145347.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="SCK20_001269"
     CDS             complement(22513..22767)
                     /locus_tag="SCK20_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG1145348.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="SCK20_001270"
     CDS             complement(23167..23436)
                     /locus_tag="SCK20_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145349.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="SCK20_001271"
     CDS             complement(23906..24361)
                     /locus_tag="SCK20_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG1145350.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="SCK20_001272"
     CDS             complement(24499..26427)
                     /locus_tag="SCK20_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1145351.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8861 a         4610 c         5614 g         7520 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taaga
//
LOCUS       DARBEG010000030        26466 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000030 DARBEG010000000
VERSION     DARBEG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.030"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            170..2230
                     /locus_tag="SCK20_001273"
     CDS             170..2230
                     /locus_tag="SCK20_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG1145352.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2313..2882
                     /locus_tag="SCK20_001274"
     CDS             2313..2882
                     /locus_tag="SCK20_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145353.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3057..3863)
                     /locus_tag="SCK20_001275"
     CDS             complement(3057..3863)
                     /locus_tag="SCK20_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145354.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            4001..5515
                     /locus_tag="SCK20_001276"
     CDS             4001..5515
                     /locus_tag="SCK20_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG1145355.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5918..6901)
                     /locus_tag="SCK20_001277"
     CDS             complement(5918..6901)
                     /locus_tag="SCK20_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG1145356.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6906..7553)
                     /locus_tag="SCK20_001278"
     CDS             complement(6906..7553)
                     /locus_tag="SCK20_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1145357.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7638..8270)
                     /locus_tag="SCK20_001279"
     CDS             complement(7638..8270)
                     /locus_tag="SCK20_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1145358.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8286..8837)
                     /gene="coaC"
                     /locus_tag="SCK20_001280"
     CDS             complement(8286..8837)
                     /gene="coaC"
                     /locus_tag="SCK20_001280"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG1145359.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8834..9571)
                     /locus_tag="SCK20_001281"
     CDS             complement(8834..9571)
                     /locus_tag="SCK20_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG1145360.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9568..10557)
                     /locus_tag="SCK20_001282"
     CDS             complement(9568..10557)
                     /locus_tag="SCK20_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1145361.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10815..11357
                     /locus_tag="SCK20_001283"
     CDS             10815..11357
                     /locus_tag="SCK20_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1145362.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11374..12459
                     /locus_tag="SCK20_001284"
     CDS             11374..12459
                     /locus_tag="SCK20_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145363.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12471..13277
                     /locus_tag="SCK20_001285"
     CDS             12471..13277
                     /locus_tag="SCK20_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145364.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLLLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13278..14105
                     /locus_tag="SCK20_001286"
     CDS             13278..14105
                     /locus_tag="SCK20_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145365.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14102..15175
                     /locus_tag="SCK20_001287"
     CDS             14102..15175
                     /locus_tag="SCK20_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1145366.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15603..18299
                     /locus_tag="SCK20_001288"
     CDS             15603..18299
                     /locus_tag="SCK20_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1145367.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18376..18864
                     /locus_tag="SCK20_001289"
     CDS             18376..18864
                     /locus_tag="SCK20_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1145368.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18876..19370
                     /locus_tag="SCK20_001290"
     CDS             18876..19370
                     /locus_tag="SCK20_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1145369.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19391..20200
                     /locus_tag="SCK20_001291"
     CDS             19391..20200
                     /locus_tag="SCK20_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1145370.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20197..21018
                     /locus_tag="SCK20_001292"
     CDS             20197..21018
                     /locus_tag="SCK20_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1145371.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21069..23168
                     /locus_tag="SCK20_001293"
     CDS             21069..23168
                     /locus_tag="SCK20_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG1145372.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23165..24283
                     /locus_tag="SCK20_001294"
     CDS             23165..24283
                     /locus_tag="SCK20_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1145373.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24322..25200
                     /locus_tag="SCK20_001295"
     CDS             24322..25200
                     /locus_tag="SCK20_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1145374.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25391..25543
                     /locus_tag="SCK20_001296"
     CDS             25391..25543
                     /locus_tag="SCK20_001296"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1145375.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8391 a         4566 c         5565 g         7944 t
ORIGIN      
        1 aaaattaaaa agtacataag ttattattcc gattttcttg aaacaataaa aaatcaaaaa
       61 aaatactctc ctaatagtcc atcttctttt gattttattt atataatgaa atcatcttat
      121 gaaaattact acttcatgtt tagacaagag tagggaggac taagaataaa tgtgttcaaa
      181 aagacgttca aaacaactaa ataaacacgc ttgtcgttta gataaggaac ggtcgtgttc
      241 tgaaaaacac tacgttaagt ttatgatgcg cacgatgctg atcagtttat ctgtaggtgt
      301 ctatatgcat gtacccgttc aggcaacgac acaaaccatc aaggtcgtac gtacctctgt
      361 ttctgagacc actgattccc ctatcactat tactcatact ttcactgatg caaatggaaa
      421 acagctactt cctgcattga cttcctataa taaagtcagt tatcctccaa cgataaacca
      481 ctactggtta attcctaaca aaacattatg gacatcggaa accggaagca gtgtgccgct
      541 ttctatcttt tccgatatga ctgatcctac aaaaggtgat cagttaaaac agatcactga
      601 tattttgaat catgctttga cagatataaa ggacggtgga agcgcacaag tttcttatgt
      661 atatgaaaaa gattattcac agtttgaaag aaagaaaatc gatgttctgg cagggacaaa
      721 agtttcgata tctgatctgg tagaaaggct gaagaacgca gatggaacac aaggagatat
      781 gtccaaagta atttcaaaca atgggaatat cgtggatacg actaatacag gaacatctac
      841 tatttccttg tcctattttg atccaattgc tttaaaaaca atgactgcat ctgctgtgat
      901 ccatgttcgt gctgatcaaa cagctatcaa aggaaaagat cttcacttaa gaacaggaga
      961 gagttacgcc cctaaagatc ttgttgaata tgtaacaaaa agcgatggaa cttcaggaaa
     1021 taaagaagaa atcaaaatga acgggacagt agatactaca actccgggag aatatcccgt
     1081 tgtattaact tatatagact cgaaaacatc aaatgtggca gaaacaacag ctgtagttta
     1141 tgtagaagca tctgctgctt catcgactgt catcactcat tttatagata gtcaaacaaa
     1201 taaagaactt caagcagaaa cacatatgag tagtgtaaat cgtattcctg atttgcgcct
     1261 acctgctgaa ttttctgatc gagtgccaaa tatagattta tcgacttatg aacaaaatgg
     1321 gaaagtcact tctatttctg attggatgca agcaaacgga ttagacagaa aaagtcactg
     1381 gtcggagatc cttgaagtac ttcactcaga actgatgaat aataggaagc aatcaggagg
     1441 ggaaattacc tttacttatg tttatatacc agacaatcgt tcattagaag gtcatgatct
     1501 tattgttcat ccaaacgaaa aaataaacaa agaagagttg atccaatcag caacaaattc
     1561 cttaggtaaa cccgtaccgt taaaggatgt atcggttaca atcaatgaag taccattgga
     1621 ggatggttat ttgaccaaac aaacaggtga ttttacgatt atgtatagtt atcatgaccc
     1681 gtattcactc caagaactcc atgctcagtc actattacat gttttaccat taaacaaaca
     1741 gatagatcaa aaagaacaaa aaagacgaga agaaagcttg tcagaaaaaa aacaacaaat
     1801 ggctcatgca gtttctccaa cagggaaagt aaaacaaaca agaagcctag agttagcaag
     1861 aatatctaga aaaatctcag aatctaataa aacgataagg caagaagctc atttggcccc
     1921 acaagtttct ggattggaaa atgaaatttt agagcctaaa ataaaacaac aatcagaaaa
     1981 agaatttgtt gctaaacagt cggaagatca gaaggaatca gatgaccaga aggaaacgaa
     2041 gaaaaaacgc ttgtcaacta ttgattccac cgatgagcat ctgcagccgc aagtaccact
     2101 tcctagtccg ggaacacccg cacctggtgg aggtattcct tcttctgctt taggagattt
     2161 catgcgaggt atggcaggaa catgggtata cgctaatcga aatgaatttg acgcagatga
     2221 ctggttctga tttatatctc tatctgttat agtcaaataa gtagctgagt catatgttat
     2281 ttggcataat ggagacgagg agggatagaa aaatgttaga taaaacgtta ccttacgcag
     2341 aaatctggat gacaagacca cttcaacaaa atgttccaat cacgccttta gcggaaggtt
     2401 ttgaatttcg attttatcaa aaagaagatg aatctgcttg ggcggagatt gagacttccg
     2461 ttggggaatt tcaaacgaca gatgaagcac ttgagtattt tcaacaaaac tttgctcctt
     2521 atcctgaaga acttgaaaga aggatgtttt ttgtagagac aaaaaaagga gaaaaagtag
     2581 caacctgtac cgcttggcga aaaaaatcaa gagagcaagt gtatccggtt tttcattggt
     2641 tagcagtgaa accagattac caaggaaaag ggctcgcaaa agcattgact gccagagtct
     2701 tgcaagaatt tcctaccctc catacggacg gaccaatcta tcttcacaca cagacatgga
     2761 gccatcaggc gattgctctt tataaaaaaa tgggttttac ttttattgct gaaaatctag
     2821 acggcacacc taatccagat tatgaaaaag ttatgcaggt tttaagtaaa ttagataaat
     2881 aacgaaatgg tgaaataagt cctcagtatt gggataaaaa acgaaatagt cattcctcat
     2941 atcttacttt tttgagttaa tactatcgga taactaatgg catgccatac attcaaaaaa
     3001 gaaggggcag tgacaaaagt attgtcactg ccccttctct tgttttttag catgttttat
     3061 cgtttgattt ttttaggaat aaaattcttg atgaacgtaa atttttggta atcgtttcga
     3121 ctatagaggt aaccaaatac actgaaaaca actgctccaa taaaattaac aaataaatcc
     3181 ttcatagtat ccatgattcc cacgtctaag taaccatttt ggatgattgt tttatctgtt
     3241 ttaccatctg cattttgact ttcaatgatt gtccgagtga tatctggaac atgatagaca
     3301 gtgtttccgt ctttacttaa ctccacacta ttgatttgct ggatgatcgt atctttttgc
     3361 atatcgaatt gcagtaattt atctgctgaa aattccacaa actcccacat aacaccaact
     3421 gtcatcgaaa aacagaagga aacaactgct agtaataaag gagtcaagtt catggttttt
     3481 gtatttttat tgagcaaata aacaagggaa aagccgacac ccccagctaa aaatccgctg
     3541 aatgcatgta gaaaagtatc ccacattgaa aaatagccat aaaaatcact taactctcct
     3601 aaaactgttg aactaaaaat aaaagataac aaaatgattt ctagcatatt tggaatctgt
     3661 atctttaata tcttttccag aaaataaggg atttcaaata aaattaatgt cagtaataaa
     3721 agcattgtat ttccccagtg ttggttccag acctgatcca ctagcataat aacgacgatg
     3781 atacgtaata aaatatagat tccgtttgtg attctacctg cagtttttgt actgttcgta
     3841 aaaaaggttt tcaaatgatt cataaaataa ctccttcatg tatatcctta attatactaa
     3901 gaagcgtgtg tttaccaaag ctatatgact taaagaaaaa cagagttgat attgtgaaaa
     3961 aaatcactta atatagtata aggagaaaag ggggatacaa atgaaaaaat tagttgctgg
     4021 tttattaaca gccgttgcac ttcttgctat gagcgcatgc ggcgcagatc agaaaaatac
     4081 agcgcagaca agttctagta cgaaggaatc tgccgaagta tcatcaggcg cttctgaaca
     4141 agtatataca gatccaaaag aaatgaaaga atcttatgat gtcgtgatta ttggagctgg
     4201 tggtgcaggg atggcagcag ctatcgaagc aaaagatgct gggttgaatc cagtaatttt
     4261 ggaaaagatg cctgttgccg gaggaaacac atctaaatca tcaagcggta tgaatgcttc
     4321 agaaacaaaa tttcaaaaag aagaaggaat caaagactcg aatgatgcct tctatgaaga
     4381 aacgctaaaa ggtggtggag gaacgaatga taaagaaatg cttcgatact ttgtcgatca
     4441 ctctgctgat gcggtcgatt ggctagatca aaacggtatc aaactggaca acttaacgat
     4501 cactggaggg atgagtgaaa agaggacaca tcgtccgtct gatggctcgg ctattggtgg
     4561 atacttagtg gaggggttat tgaataacgt ccatgagcgg gaaatcccta tttttgtcaa
     4621 cgcagatgtg acagacatta agaaaaatgg ggaaacggtc aatgaagtga ctgtacatgt
     4681 tcaaggagaa gaaccaaaag aggtcaccgg aaaagcaatc gtagttacca caggcggttt
     4741 tggtgccagc aaagaattta ttgaaaaata tcgtcccgat ttagctgatt atattacgac
     4801 aaatcaagaa ggatcaactg gagatggtat caccatgatt gaaaaattag gtggacaaac
     4861 cgttgatatg gataaaatac aaattcatcc aactgtgcag caggatgagg gggtcttgat
     4921 tggtgaggtt gttcgcggag aaggagcaat cttggtcgat caagagggga aaagatttgt
     4981 caatgaaatg gatacaaggg ataaagtatc tgcagcaatt acagcattgc ctgaaaaatc
     5041 agcttatttg atttttgacc aaggcgtgcg tgatcgtgcc acagctattg aattttatga
     5101 ccaaaaagga tatgtcacaa ctggaaagac aatggaagaa ttagcagaaa aaatcgattt
     5161 accaaaagaa acactagcaa aaacagttga tggatggaat acggatgttg cggagaaaaa
     5221 agatgctcaa ttcaaccgtg ccacagccat ggaacatgac ttatcgtctc cgaattatta
     5281 tgcgatcaaa atcgcaccag gaatccatta cacgatgggc ggtgtgaaaa tcaatacgaa
     5341 cactgaagta ttagataaag aaaaccagcc aattaggggg ttatatgctg ctggtgaagt
     5401 cacaggtggc ttacatggaa acaaccggat cggtggaaat tcagttgcgg agatcattat
     5461 ttttgggcga caagcgggca tacaagcagc tcgatttgct tcggaaaatg aatgaaatag
     5521 aacgtaaata aaaagaggtt gtgaaatcag cgttttgagc taataccgag ggtcagctga
     5581 ttgaacactg tttagtgaag aggttgtgaa aaagctgccc tgcatcaagt aataagaaca
     5641 gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc gcagatgcaa
     5701 gcttttgaac accgtttatt cggaaaaaga gcgtgggaca aaagtaaaga acacttttgt
     5761 ctcacgctct aaacacgtat aaacggcgga agcagaagaa attccttcgg aaataagctg
     5821 aaattcacaa aaatttgaaa agcaattttc gtgaatttct tcttatttct tggagttaaa
     5881 cgcttctgtc ccagcctctt ttttgatcag catgatttta ttcagttatc ttcaaaatga
     5941 tttttccgat gtttttgttt gcttccatat attcatgcgc ttgtttgact gcgctgaatt
     6001 ggaatgtacg gtcaatgatt ggccgaatct tgccattttc tagaaaaggc ccagcttttt
     6061 gcataaaagt ttgtgcgagc tctgctttat actgatcact tctaggtgtc aataatgtac
     6121 caagcagttg gacacgtttc ccaatcaata caccaaggtc tatctcaggc acgatggtcc
     6181 cacctagcat accaatcaag acccaacgtc catcaacggc tatagaagct aaattctttt
     6241 cccagtaaga agcgccgatg aaatccaaaa taacatcgac tccccgacct tcagtgattt
     6301 ttcgtacctc ttcttgaaag tcttgttttt tatagttgat caattcatct gcaccaagtt
     6361 ctttacaaaa ggcgagtttt tcttctgatc ctgcagtaac gatgattttc gcgtgagaaa
     6421 gctgtctagc taattggatc gctgctgttc ccacaccact tgctcctgca tggatcaaga
     6481 cagtttcgcc ttcttgtaat tttccaagcc agtaaagtgt ttgataagca gtcaaaaaaa
     6541 cttctgagat tccagcagca gaaacatagt ctagcgtatc agggagaagg attgcacgat
     6601 cggcaggcat cacagcatat tcggcataac cacctagatt gactaatccg tatacttttg
     6661 tcccaggagt aaaagtggag atatcacttt tattctcaac tacctctcca gctacttcga
     6721 ctcccaaaat agggtaggga ggtttcaaag agactgattc tcttttcata atatctgttc
     6781 gattgacagc tgctgtatgg actttgatca gtaattcgcc ttgttttggt ttaggaacag
     6841 gcacttcaat ttgttctaat acatctacag aaccaggatg cttgattgaa atggcacgca
     6901 tggatctatt cctcctttgc ccattcttgg aatgctgtat tcaactcttt gctaccttca
     6961 gaaagtacta gagtcgtacg agaaatatcc gtttcttcca ctagagcggc aagtacagca
     7021 gcaactgtag agcgagtgat cgtttttgat gccttctcat taggcttagt taattgaatg
     7081 gaagtaactt cttcatcgtt agtcaatgtg actggttgaa gaatgacaaa atctaagtta
     7141 gttcgtttca gccattcatc tgcataatgt ttagtaatat aataatctgt catggactca
     7201 ggccattttg ctggttcatc tgcataaaca gcactgacca tcagatagcg agagacatgc
     7261 gcattttcag ctgcgatcac tgttttgatt gcgccatcca agtctacttg cagaagattc
     7321 tttccttgag agccggcagc aaaaatcaat acatcgatgc ctttgaatgc ttcggtcatt
     7381 ttttccacgg accaagttaa atcaaaagag acatacgaaa cattctcttt ttttactaat
     7441 gtttgggaag caacatctct cacccctgca aaaatttcat gggaacttgg agctaaatct
     7501 tttatcaggt gtcggccaat ttgtccgttt gcacctacaa caaaaatttt catttggatc
     7561 atcctttcga cgtcaaatct ttccattcaa tagtaacacc aaaagagaaa aaaatcagag
     7621 atcggctacg atgactatca gttagtaaga gaagggtgct tgaagtatct taacaagaca
     7681 ttgcatactg ctgcagtcgc aagacctgcg acgattcctt caggtatacc acttgtaaaa
     7741 ataaccgcta gaatagctga ataaacagca tttaaatttg ttccgatgac tttggcataa
     7801 tcttgctgga ataaaaagta gatcatattc atcactaaaa tcgtattggt caatgaaccg
     7861 gaaagtccag ctaagaataa agaaacggaa tctgcttttt gtttcattag tttgcgcaga
     7921 cctttataaa cgaaataagg aaccacccca atcaagatgc gaggaataaa cgcgatcagc
     7981 aaggctttcc agcttccatg ttctgttccg atgactggga taaacggaga aaagacaaaa
     8041 gacaaaggtg tttggataac tgtgctacgg atcatgctga tcagtccaaa tgtaccgcca
     8101 agaaaagcac ctagtcttgg tcctaaaacg atcgaagcaa tgattacagg aatatgcatg
     8161 gttgttgcat tgattggacc gatagggata aaacctaaag gcgtaacagc taataaaatc
     8221 aaaatagcta aaaacatcgc agttaatgta aagttttttg tatttttcat aagtactcct
     8281 tttttctaag catttctttc attaagagtc agcatgacct gttcgatgat cgtctgaata
     8341 tcagctaagg caccttttcc aaaatctcca caagctaaaa gagattctct tggttcaatt
     8401 tcctgatagc cgacagatct caatgtttcc agattacgct gagtgatcgg gttttgatac
     8461 atattcgtgt tcatggcagg ggcaatcaat tttggtgttc ccactggaag cgccattgcg
     8521 actgttgaaa gaagatcgtc tgcaatgccg tttgctagct tgccgataat gtttgctgta
     8581 gcaggcgcga tgagaaacaa gtcagcttgt tttgctagtt cgatatgatt gattacctgc
     8641 ggatttgatt cttgcatgac gtctgtatgg actgcgcgct tcgacagaga ttgcaaggtt
     8701 aatggcgtga taaaatgtgt gctgctttta gacataatca catctacctg ggcacctaat
     8761 ttgacgaatt gacttgtaag atcagcgctt ttgtaagcag aaatacttcc agtaacgcct
     8821 agtaagatct ttttcatttt gattcctcca gtctaatttt ctcagtaatc aatgcagcaa
     8881 tctcagattt actttgagct tcttccactg ttccatcttt agataagaga tagccgtggt
     8941 gtgtctcgcc atggatttcc gtcaaatcat ttgcgaaaat aaaatcagtt ttgttattta
     9001 tcagactatt ttttgcgact gccagaagag attcctttgg tacatctact aatagtttga
     9061 aaccaatgat ttttgctttt ggctgccatt cacgtagata agaaatgatt tttggtgttt
     9121 tcttcaaaac gagcacaaga tgctcggtgt tagaagagat ctttgaaaca gtggtatctg
     9181 tctgttcatt ttccgcaaac caattgtcca gttcttcttg cgttgaagaa gtgggtaggt
     9241 tttttgccag ctgttcttct gaaaaactaa aagcaggagt aaaatcactg actgccatac
     9301 tgtggataat cgcatcgtag tgttcttttt tcgttagttc ttctaattgt aaaaataagt
     9361 ccagtgcaga ttcgatcgta taaatcgtta tattggagtg tcttttcggt ttcagtgctt
     9421 gtttcgtagt cacgtaatcg attgtggtat ccggattggc agctaaataa tcagctaatg
     9481 cttgccctaa tcgtcctgtc gaatgatttg taatagcacg tacttgatcg attttttcag
     9541 tcgttcctcc agctgtgatt agaatcttca tttatggtca cctacattta ctagtgttct
     9601 tccttggtgt gttccagcaa gtagtttaga tgctgtttca ggcaattgat ccagagtaat
     9661 ctcttggacg accagttcat ctgcaatctg caaatccgcc atctgttgcc ataaagaaag
     9721 acgttgatct attggtacat tgacagaatc aattccaatc aactggatgt ttcgcagaat
     9781 aaaaggcaag acagtcgcgt ttagtttgat tcccccagca ttgccacata aagctactgc
     9841 accgttataa gagatcaatg gaagaagact gcttagctgc tcgccaccta ctgtgtcgat
     9901 gacatagtcg atttgctgtt ttcctaaagc ttttgttgtc tctggcagaa attcatcagg
     9961 tcgtacgact tgcaaagcac ctagactttt tagccatgtg acagactctt cttttctgga
    10021 aaaggcaatg attgactgga atcctagttt atgtaacagg gctatcgctg tactgcctac
    10081 accgccagaa gcaccagtaa cgacaacttt tttatcggag gtcatacctt gacggattaa
    10141 agcgttcaca cacaacatag cagtaaaccc tgctgtacca aggatcatcg cttgacgcag
    10201 cgtcatattt tttggtagag gaactaacca gtctcctggt actttttgat attggctata
    10261 accgcctgga tgggtgacgc caagcccaaa accagtagca agtacaagat cttcttttga
    10321 aaaacgtgga ttgttacttt ggacgacagt tccggataaa tcaatccctg gagtcatcgg
    10381 atatgttcgt atcacctgac cagactcgct cattgccaat gcatctttat aattgacatc
    10441 agaataggca atttttacca agacttcatt ttcagccaac tcagtaaatg gcagatcact
    10501 gattgctgta tggaaaacag gttcctttat tacctgaaaa gatcgaaaag cgttcattcg
    10561 tgagacactc cttttaaaat tttcttttat cataccataa agcctactct tttattcttg
    10621 ttcttccgta aatttagaca aaaattaggt gaaaaataaa gaatcaaaca actagaattt
    10681 attccaaaag ataaagaaat attcagcaaa aaaaatttta caaagggttg cctttttcaa
    10741 taaatttcgt atactaatac gagtggtagt aaacaggaag tttactatta gaaaacttcg
    10801 attcggagga aattatggaa attggtgaga agttaagaaa tttgcgcatc caaaagaatt
    10861 tgacacaaga agaactaggc gaacgcacag atttgtcaaa agggtatatt tcccagttgg
    10921 aacgagattt aagttcacca tcaatggaaa cattcttttc tattcttgaa gtcttaggtg
    10981 tgactccaga agaatttttt catcaagaag cagcgaatct gcaagtggtt tattcaaaag
    11041 aagatcatac ggtttatttc gatgaagaaa acgggtatga attggaatgg ctcgtggctg
    11101 attcgaatga aaaagaaatg gagcctgtct tgctgacttt tgaaggtgca ggagaatata
    11161 agacgtttga accttcatta tctgaaacgt ttatttttgt attagaaggg gaattagagt
    11221 taagcttagg agagacggtc tatgtggcaa aagcaggaca gtcgatctat tatcaagcga
    11281 caaaacaaca tcaattaagg aatcatctca aaaaacgaac aaaagtattg attgttgcaa
    11341 cgcaatcata tttgtaatat agggggtaaa aacgtgggga aaccaatcat ttcatttgat
    11401 caagtagtga aacgctatga tgatgaaaca gtattaaaaa aagtaagttt tgagatcgaa
    11461 caaggaaaat tttatacact gcttggtcct tccggctgtg gtaaaacaac gatcctacgt
    11521 attatagctg gattcaccga agctacagaa ggggacatct attttgaagg gaaacggttg
    11581 aatgatttgc cagctaataa gcgacaagta aatacagtct tccaagatta tgcacttttt
    11641 cctcatatga atgtttttga taacgttgct tttggattga agatcaagaa aatgtccaaa
    11701 tcagatatcg aaaagaaggt aaaagatgct ttgcgtatgg tccagcttcc tggatacgaa
    11761 aaacgggaga ttagtgaaat gtctggtggt caaagacagc gtgtagcgat tgcacgagca
    11821 attgtaaatg aaccgaaagt attgctatta gatgaaccgt tatcggcgct tgatttgaaa
    11881 ctacgtacag atatgcagta tgaactgcgg gatctgcaac aacgattagg aatcacgttt
    11941 atttttgtta ctcatgacca agaagaagcg ttggcaatga gtgatgagat ttttgtcatg
    12001 aataaaggga aaatcgtcca aagtggtaca cccgtagata tttatgatga gccaatcaat
    12061 catttcgttg ctgattttgt tggggagagc aatatcgtgg acggtgtaat gatcgaagat
    12121 aatctagttg agtttgttgg taagcaattt gaatgtgtcg atggcgggat gcgtctaaat
    12181 gaaccagttg aagttgtttt acgcccagag gatttgacga tcacgacacc agataaagga
    12241 aaactagtcg taacggtcga tactcagctg tttcgtggtg tccattatga aatcatctgt
    12301 tatgatgaac agcaaaatga atggatggtc cattcaacga aaaaagctaa agaaggaagc
    12361 aaagtaggat tggcctttga gccagaagat atccatgtca tgcgtttcaa cgagtctgaa
    12421 gaagaattcg atgcacgtct tgacagctac gaagaatagg ggggacaaga atgaaaacaa
    12481 tgcgcaggat ctattccgtc ccttatattt tgtggctcgg tttattcgtg atcgcgcctg
    12541 tattaatgat catttatcaa tctttttttg atatgaatgg gcaattcacg ctgaataatt
    12601 atctatctta ttttacgtcg gggacatcgc tttctatgac gatcaattct gtatggtatg
    12661 catttttgat cacgtttttc acgctgatca tcagttaccc gactgcttat tttttaacaa
    12721 aactgaagca caagcaatta tggctgatgc tgattatttt gcctacatgg gtgaatctgc
    12781 tgcttaaagc ttacgcattt attgggattt tcagtgtgca cggcagcgtg aatcatttct
    12841 ttgattttat cgggatcggg ccgaaacaga tattattcac agacttcagt tttctgttcg
    12901 tggcaacgta tatcgagatt ccatttatga ttatgccaat tttcaatgca ttggaagaga
    12961 tgaatccttc tttgatcagt gctagtagag atttaggcgc gaatagcttt gagaccttcc
    13021 gacgagtgat ttttccgtta ttattaaatg gtgtgaaaag tggtgtccaa gcagtattta
    13081 ttccttctct ttctttattc atgctgactc gtctgattgg cggaaatcgg gtgatcactt
    13141 taggaacggc tatcgaacag catttcttgg ttacacaaaa ctgggggatg ggttctacaa
    13201 tcggtgtgat tttaatcatt gctatgttca tcgtgatgct cctgacaggc gagaagaaaa
    13261 aaggaggtcg ctcataaatg aaaaaattta aatggtcgta tctttattta gggatcgtct
    13321 ttttattctt atatgcaccg atcttctatc tgatttttta ttccttcaat gaaggaggaa
    13381 cgatgaacag ctttacagga tttacttggg aaaattatga agctgttttt gaagatacac
    13441 gtttgatcat tatcgtattg aatacgttca tgctggcttt cttatctgca ttgatcgcca
    13501 cgatcatcgg gacatttggg gctatgggaa tctattacac gaaaaaacga cgtgcacgga
    13561 cagcgttgtt gagcatgaat aacatcttat tagtctctcc tgatgttatt atcggtgcta
    13621 gtttcttgat tttctttaca ctactaggat tcggattagg ttttcagagt gtattgcttt
    13681 cccatattgc tttcagcatt ccaatcgtcg tcttgatggt cttgccaaaa ttgcaggaaa
    13741 tgaacgattc aatggtcgat gcggctagag accttggtgc taataatttc caagtagtca
    13801 aaaatatcat tttgccttat ctttctccag gaatcatagc tggatatttt atggcattta
    13861 cctattctct tgatgatttt gcggttacat ttttcgtcac aggaaatggc tttacgacgc
    13921 tctcagtaga gatctattct cgtgcaagac aaggaatcag tttagaaatc aatgcgctaa
    13981 gtgcgttagt tttcttgttc tccatgatat tggtcattgg atactacttt attagtaaag
    14041 aaaaccgtcc aaaacggctg aagaaaaaca gaagagtacg cgagggggtg gcacggctgc
    14101 gatgaaaaaa ttacaatctc tgtttttagg tattctcatc attattgcgg tgctgctgct
    14161 tggtgttcgt caacttgaaa aaacaagtgg catgagtgga gcaaaggtac ttaccatcta
    14221 taactgggga gattatatcg atcctagttt gatcacgaaa tttgaaaaag aatatggata
    14281 caaagtgaat tatgaaacat ttgattctaa tgaagcgatg ttcacaaaaa tccagcaagg
    14341 tgggacaaac tacgacatcg ctataccaag tgaatatatg atccaaaaaa tgatcaaaga
    14401 aaaactagta cttccattag atcattcgaa gatcaaagga ttaaataata ttgatccacg
    14461 atttttagac ttggattttg atccagataa tcagtattcg attccatatt tttggggaac
    14521 tttagggatc gtatataacg ataaattctt tgatgcaaat caaattaagc attgggatga
    14581 tttgtggaag ccggaattaa aagacagttt gatgctgatt gacggtgcac gagaagttat
    14641 ggggctttct ttgaatagtt taggctattc tttgaatagt aagaatatgg cacagttgac
    14701 agaagcagca aataaattga atcatttaac accaaatgtc aaagcaatcg tggcagatga
    14761 gatcaaaatg tatatgatca atgaagaagc ctccgtggca gtgacatttt ctggcgaagc
    14821 tgccgatatg atgtgggaaa atgaacattt acattatgtg atcccaccag aaggatccaa
    14881 tctttggttt gataatatcg ttatgccaaa aaccgtgaaa aataaagaag gtgcctatga
    14941 tttcatcaac tttatgctag aaccggaaaa tgcggcacaa aatgcagagt atattggtta
    15001 ttctacacca aatgaagaag caaaagcgtt attgccaaaa gacatttcgt cggacgaaca
    15061 attctatcca tctgatgaca cgatctccca tcttgaagtc tacaaagatc taggatcgaa
    15121 atacctaggc atctataatg acctattctt agaattcaag atgtatagga gataatggca
    15181 aaactcagta agaatgggga cttatcaacg ttctaaagag ctaaaaatcg tcctttttct
    15241 atctgagttt tccaatcttc taccaaaagc ggatgaaacg ccgctttttt tgtgttattt
    15301 tattttttct accacgtttt tctaccagag aaaagtggac aaaaccattg taaaataggg
    15361 cccgagacgg gctagtaaat ctaagattga aaaagttaca gaaggataaa gtaattggaa
    15421 actatgcaaa tatacttaac ccttctgtaa tctattaata aaactaatat atgaatccag
    15481 ttcaatattt gtagctatga aaaaacataa aaatcatatt agataaaaac taaattggta
    15541 gataaattat tttgtcagat atctgaggca aatattcgac tatttgtgtt tcaaagtaaa
    15601 aattggaata caaatataga atagacatat tgcagccgat agtttattat gcttttagac
    15661 acattaaact ggaggaaaaa atgaaaaaga gcgctaaaga tcgagttttt gatgagctga
    15721 tacgactaac agggaatatg tcttctgtca ctgccgagaa aatttcgaag aatctaggta
    15781 tctctcgtca gaatgccagc cattatttga cgagattagt ggaagataaa aaggtcgaga
    15841 aattaccggg aaagcctgtt ttgtggaagc ctttagatga atatgcggtt gtagataata
    15901 cggagcaaat aaacgaagcc tttcattctg tagttggtca tgacggaagt ctgagagagg
    15961 tcattcaaaa atgtatagcc gctgtcaaat atccgcctaa cggactcagt gtattaataa
    16021 atggagccac gggtgttgga aaaagttttt tagccacaaa aatttttgaa tacgctatcc
    16081 atgagcaaat tattgaaaaa gatgcaccat ttgctatctt aaactgtgcc gattatgcgg
    16141 acaatcccga actgctttcg gcaacactgt ttggatacaa gaagggggcc tttacggggg
    16201 ctgaaaagga taccgatggt ttattggcaa cggccaacaa cggctatctt tttctcgatg
    16261 aggtccatcg cctatcgaaa gaaaatcaag aaaagctctt tctatttatt gatactggaa
    16321 actatcgtcc agtcggtgaa aatgtaaatt ggcattctgc taaggttcgt tttattttcg
    16381 ccacgacgga aaaaggtgaa aattatttat tagacacctt tgatcggcgt attcaaatta
    16441 gtgttatgtt gccaactttt gaagatcgcc ccattcgaga acgattagag ctgattcaat
    16501 tattttttca aaatgaagcg gatgttttac aaaaagatat tattgtttca agagaagcct
    16561 tgtctttaat gttatctcat ccctttagtg ggaatattgg taagctgaag aatatcatta
    16621 aaatcagttg tgcggatgtg tatagtcgaa cacaagaaga accattaacg attgggaaaa
    16681 atgagatctt gattcagtta aatatgctgg aatccgaagt agaaagcttg ccgattgcta
    16741 gtttattgat aaagaaaaat caaaagataa aaacggatcg aatcaaaccg tttgattatg
    16801 attcgatttt ggaaaaaatt actcagctga taaatgaagg taatttcaat gaaaattttt
    16861 cttcgataaa gaatgaagta cagcatttgg tgcatcacat tgataaggat tcttttttat
    16921 caatgagtag aaaattgatg attcagatgt ttagaaaggt ctgggtatcc gttttaggaa
    16981 aaacgtacgg attgacgacg agtggacctg ttgcagaaat tattagcaga tcctatgtca
    17041 tttccccaag ctataagaca gacttaaccg ggataaatcg tacattagct gcaaatctac
    17101 ctcggacgac ttacttatgt aatcagttct tggagcattt accaccgctc tcagaaaagg
    17161 acgagaactt tctaagactg cttattgtcg tgactttatc tgattatgta gatgaatcca
    17221 tcgagctgaa gggcctgttg cttgctcacg gagatagtac ggctagcagc attcaagcag
    17281 tagtgaatca attatgcggc aattatgttt ttgaagcgtt ggatatgcca gtagagagta
    17341 atgtgtcaga gattgtcgaa tcgacaagga atttcgtgaa acggcaagtg aatacagata
    17401 gtctagtgct gatcgtagat atgggctctt taaatcaaat ttacacacag atcaagaacg
    17461 atttaaaagg ggagttactt cttctgaata atttgacgac ttccattgca cttgatattg
    17521 gcttgaagat gacgatgaat gcgccattta aacagattgc ggaggatgca gcagacaagt
    17581 attcaatcaa tgttcaatac tttgaaggct tttctcaaag tagcaacatt atcatttcct
    17641 gtatgtcggg tttggggatt tcggacaaat tgaaagatgt ttttcgtcat catttatcgg
    17701 atgaatccat cgaagttttc acgaaggact acagagaact acgtgaatta atcgaccaaa
    17761 atgatgaaac ttattttgac aagacaaaat tggtcatcac gacttcggat cttccacgtt
    17821 ccttttcaat tcctaatatt aatgtttatg acatcttaga cggtgatgga gcagccaatt
    17881 tggagcatat tttgagtcct gaaatgaagc cggcagaatt taaccgcttg attcaggagc
    17941 tagtaaaatt cttttcgttg gaaggaattg cggaccgttt gaactttctg aacccaacga
    18001 tcgtgatgag tgaagtggaa acagtcattt ctaaatatga aaattactat catttcactt
    18061 taaatggccg tgtaaaacta aatctctata tgcatacggc attgatgatg gaacgtttat
    18121 tcttggctcg gacagaaaaa tctgaagaac cgaattatga tttgaaacca gatgaagatg
    18181 aattttatta tgtttctaga agtatttttc aatcaatgga gatgaaatat aattttaaag
    18241 tcaatgctta tgagctttcg ctgctctatg aattattagg accgtatatt caaaaataaa
    18301 gtgtctaaaa aagtgtctaa tctgtttttt ttaggttaga cacttttttt aatgtgaatt
    18361 aacgtgatta aaccaatgtt tttaaaagtt ggcacaggaa ttgcttatat aaaagtgaac
    18421 agaaagaaag gaattaacca tatgaagaaa attctaatag cgactcacgg tcacttagct
    18481 tcaggctttc ttagcagtat tcaattactg acggggaagg ttcaagagat tacggtgatt
    18541 aatgcttatg tggatgagtg tgattttgaa caggagttga atgtatttgt aaaccaggtg
    18601 aacgagcagg atcagatttt tgtttttacc gatttatttg gcggaagtgt caatcagaag
    18661 attaccaaaa cctttttgga gaaagagatt gctgtaacta ttttgtctgg ctttaatctg
    18721 ccaattctgc tcgaaatagt attggcaacc aaggaattaa caaaggagga tattcaggaa
    18781 ctggtaaata aatgccaaca ggaattaaag atgaccattc ttgatgaggt acatgtagta
    18841 aatgaagagg aagatttttt ttaggaggaa gaaaaatgat taaagcagtg cgaattgatg
    18901 aacgattaat tcatggacaa gtagcgatga cttggacaaa gagtctaaat ttaaccgggt
    18961 tagtagtagc aagcgatgag gcggctacca acgatattca aaagatgaca ttgaagatgg
    19021 ctgcaccatc gaatgtcaaa gtcattatta aaacagttga tgacgcgatt catttgttaa
    19081 aagatccgcg ggcgaaggac atgcgcttga tggttttagt ccctaatgtc aaagatgcgg
    19141 taaaagttgc gaaagctttc ccaacggaaa tcgaattaat gaacgtgggg aatgctggga
    19201 aaatgacacc gccatcggaa gataagaaaa ccttatcaaa ggaagtgatg ttaacgacgt
    19261 cagaaattca agacttaaaa gaattagttg aactttatcc agataccgtt tttcagccta
    19321 caccggcgat ggaaaaacgt tcagctatct ctattttgag tgatttttag aaaaaggaag
    19381 gagaaaaatt atgtatgatg catttgtagt cgctttggcc gtttttattg gaattgctgg
    19441 acatgaaatg ttcgggatgt ccatgctgag tcgtccgatt gttgttgctc cgttagcagg
    19501 agcgttgatg ggggatttgc agacggggtt gattgtcggg gcgtcactag aagccatctt
    19561 tatgggtgtc gtcaatatcg gaatctctag tacggcggag ccttctttgg ctgctgggct
    19621 ggctacagcg ttcgcaatca aaatgggagg aaacgtggat gcgattattc ccattgtctt
    19681 tccactagca gtattaggat tacagttaat gaatatgatt ttttcttttg tatgtggacc
    19741 gatggctgct aaatttttca aatttgcaaa atctggtgat caaaaaggaa tcatcaatct
    19801 gcacttcttt atgtggtttg tccactatgc gctttatgct cttattccat tctttgccgt
    19861 tttatttgga gcagatgttg tcaaaagcgt cttggataat attccagagg tcatcatgaa
    19921 cggattaaca gtcgctggaa atctgctgcc ggcagttggg atggcgatgc tgttacggat
    19981 gctttggagt aaaaatattg ctgtgtggtt tttctttggg gccgttgtta tggcgtatct
    20041 cgagttacca ttgattgcaa ttgccgcagt tggaacgatt attgcaattg ttatagccca
    20101 aagagatcta caactaaaaa atatatctgt tcatgcagtt caaagcgatg ctgctgtaaa
    20161 tgcaacgctt gaccaagaag aggaggattt ctttcaatga aattagctga tggaatttct
    20221 aaagatgaga aaaaattggt tcggaagatg ttttggcgct cgatgactat gtatgcttcg
    20281 gtgaatccgg tgacgatggg cggcggtggt ttcgcctatt ctatgatgcc cttcattaat
    20341 catttctaca aaagtgaaga agatcgagct gcggcgctgg aacgtcatac acagtatttt
    20401 tcgacgacca ttccttgcgc gtcctttgtc atggggattg cggcatcgat ggagaaggaa
    20461 aattctgaaa aagctggttt tgacgtggaa agtatcaacg cgatcaaaac gagtttgatg
    20521 ggaccattgg ctggaattgg cgattcattg ttctggggag tttggcgtgt cgttaccgcg
    20581 ggactagcga tcaacttagc caatgccgga aatattttgg caccaattat ctttttagtt
    20641 atgttcaata tacctaatta cttgatgcga tactatggcg gcgttttagg ctattcatta
    20701 ggctctaaat atattgaaaa gctgtatgcg aacgggttaa ttgaaattct gacaaaagct
    20761 gctagtatct tgggtctgat catggtaggt gcaatgacga tcagtatggt caaattcaat
    20821 accgttttga gcttttcaat gaagggcgaa gaactaatga atgtccaaga aatcctcgat
    20881 agtattttta aaggaattgt accgttattg gtaacattga gctgtttcaa attactagat
    20941 aaaaaagtcg gcgtcatcaa aattattttt ggaatggtcg tcttgggaat tttattgtca
    21001 ttacttggaa tcgcatagta ggaagctgca gctactaggc tgcagttttt ttagcaagga
    21061 gggaaaagat gactattaat gaagtaattt attcacccaa acacccattt tttaatgctg
    21121 agttaaacga cggaaatgtg actacttcaa acgaatacag ctactatcgg aatattcctg
    21181 atgtaaaggg attgaccttc gatgtaagta atcaaaagac attagcaaac cttgatgttt
    21241 gcgggacgat tcaaacgttg accttttatc gagaaaattt actaactgag gaaaaaccag
    21301 gtgtttgggt caataaacag ctctctcaaa ccaattcgtt atccttaaag gttgaagtaa
    21361 acgggattgt ctaccatttg tcagaatcgg atcatcgtat tgaagtcgat gtgattcaag
    21421 actgtttgcc tagatatatt catcattatt cgacgtttaa ggtcattgta gtatcctttg
    21481 ctcccatact ggctaataag cgtttaagta tgcttgttca acaggtttac attgtgaatg
    21541 aaacacacga aagccttcaa gtttcagtga aagatgtgcc ttcgtatcaa gagaagtatt
    21601 cggatcagca aaatgtgttg atttctcaaa aaggaaataa ggacacgatc gcacaacagg
    21661 aagtggcaag cttcgccatt gcttttgtgg acccaaatgc ttttgaagaa gtaatgactt
    21721 ttcaggaatc tgatatcgaa aaatggctga aggaaacatt gaactatttt gctcagctat
    21781 ttgggcagct gtcaatgcca aacgatagag ttgtccatct ctatcgtcgt gcactttatc
    21841 aaagcttttc tagtttcggt atgaatcgac agggacaggt cgtcggcagc aattggggca
    21901 gctatcctgc tactaatcga atttggaata aggatatgta ttacgcatca ttgccattca
    21961 ccatgtttga accggagtta tgtcaaaaaa cggttctatg gtttgatcaa tatggcgtta
    22021 agtttcctgg ttcaaaattt acaggaggta tcaatcattc tctgagtaat tcgttatcca
    22081 gtgcgttact ggctagtctt tattttgaac atactgggga tacaaaattc tttgagcggt
    22141 gtccgcaggt tctactgaat gcttcaagaa ttattgagga catattagct caacgtcggt
    22201 caggggaacc aatgcttttt ccatctgttt ggttatcaga cgcctttgca cttggaaagt
    22261 atcataccgg cagcaatctt tgtctttgga aggcctgtat tggacttgca gaaatttttg
    22321 aagccctaga acaactgagt cttagcaagc gatacaaaaa cattgcctgt aaactaaagg
    22381 aatcaatttg taaacaaatg actatcgatg gtccttttgg tgagcaattt ttggagggaa
    22441 tcggtgatga ggagaaaaca acctattcag tgaagcacta tcaaaaaccg attcttgagc
    22501 aagggttaat ctttttaagt gatgtgattg tggatggaaa aattaattta ttgatgcatg
    22561 atggagaaga atcagacact acgttgatgc ctttttatca atttttagat aataaagatc
    22621 atttatatca gaacaccatg gccttttctg ctagttcttt caatccaacc tatagcgagg
    22681 aaatcaaagg catcacttgg ggattagaat ccggcgccac ttttcctggc tttatcactg
    22741 ttttaatgag cgagctgcac aaccctcaag ctttcgtaac ccgattagaa gaactaattt
    22801 gtttggcaga tttagacggt tcgtggtggt ggtggccgta tcgcttagag gctcaacaag
    22861 gagaagtcgt gcgagacttt ggctgcggca aatgcggctg ggcatcgggt ctttttgtca
    22921 gcctaatgat cagtcaatat ttcggattga aattcaaaaa aggagtattg caaattgatc
    22981 ctgttgataa tttaacgttt tcttggaaga atttgaagtt cggtcaagcc ttcatttcga
    23041 ttgaatgtac gcaatccgag ttgagtattt caaatttgtc agaaaagcca ttgaaaataa
    23101 gtgatacgaa aaaaattctt cacgtagaaa agggaaaaac gattcacatt caaaggagaa
    23161 aaagatgagt atactaaaaa atattaccca atttaaaaaa atccagagtc gagcaatcac
    23221 tcccgaaaat ccaacaggcg agaaaggtaa agctgcgatg ttgccaagcg agctaggacc
    23281 aagccgcaaa ggtcgtggcg caattccgct gacttctggt gaagaaactg tgattgcgga
    23341 tattactgga acgggaataa ttcgtcatat gtggatgacg attcgtgatc gaacagaatt
    23401 tggcagtttt gttttacgtg atttaatctt aagaatttat tgggatgggt cggatagccc
    23461 tgctgttgaa agtcctttag gggatttctt ttgtaatggg tttggagagc gttgcgaggt
    23521 gaactcgttg ccgatagtgg ttaatcctac agggggcttt aattcctatt tcgaaatgcc
    23581 tttccaaaca tccgctaaaa ttacgatcga gaatttacat ccgaaggata tgtcctcatt
    23641 tttttatacg ataaattacg ctttggtgga cgaattggaa gaaaatagcc tatattttca
    23701 cgcaaattgg catcgggaac gcattaccaa attacaggag gattataagt taattgatcg
    23761 attaaagggt caaggctatt acgtgggtac ttaccttgcg cttacctgcc tagaacgtta
    23821 ttggtggggc gaaggcgagt ttaaattcta cattgatggg gatagtgagt atccgaccgt
    23881 aacctcaaca ggttcagaag attattttgg cggggcgtgg gcctttcatg agtacgatga
    23941 gttcggacgt ccacatgcga aaaactattc aacagccttt ttagggtatc cttaccaatc
    24001 aaatagagat gccacaagag atcgttttga aacgggaaaa ttgaatgccg ttcatgcctt
    24061 tggtgacgac gcaatgccac gacatggtct ttatcgttgg catcttcaag accccatctg
    24121 ttttaccgaa gatttatcgg tcactttaca acaaatcggc aacgatgatg ttcgcttatt
    24181 tgagcggcaa gacgatgttg ctagtgtcgc ttactggtat tcaacagaag cttcaggaaa
    24241 tactaaacgt catttcactg atcgagaacg cttgccgcgt taatgaagtg tttttcacat
    24301 attcgaaggg aggaacctat gatgatatat ggggcaattg aagctggagg gactaaattt
    24361 gtctgtgcag tcggtactga ggaattaacg attttggaaa gagtcagttt tcccacaaca
    24421 actccagttg aaacaatgaa gcaggtcatt gattttttta aaccttttaa ggaacaattg
    24481 gcaggcatcg gggtcggctg ctttggtcca attgatattc aatgcaaatc aacgagtttc
    24541 ggtcatatca catcaacacc aaaacttgct tggcaaaatt tcaattttgt aggaacgcta
    24601 aagcaatatt tcaatattcc gattgaatgg acaacagatg tcaatgcggc ttgctatggt
    24661 gaatatgttg ctggttctgg caaagggtta gcaggtgttg catacttcac tgtgggaaca
    24721 ggtatcggag gtggtgcctt gttcaatggg actttcgtgg aagggttcag tcatccggaa
    24781 atggggcata tgttgatcaa gcgacatcca aaggacaatt tttcaggaaa ctgtcccttc
    24841 caccaagatt gtttagaggg gatggcagca ggaccagcga ttgaaaaacg gttgggtgta
    24901 aaaggccaga atttattggc tgacgattcg ttttggcaga ttgaagcctt ttaccttgcc
    24961 caatgtgcat acaatacaac actgatgttt tcgccagata gaattatttt tggcggtggt
    25021 gtaatgaaac aagaacacat gaagaaaaaa gttcaggata aatttgttga gcttataaat
    25081 ggatatgttg aaataccacc aattgatagc tacattatta cgcccgaatt aggtgataat
    25141 gccggaataa tcggcggatt agcattggcc agaaaagcgg taagaaataa gcaaccataa
    25201 ttaatgaatg tttcattgat actcatgtca aaagtattta accgagtaat caaatgatca
    25261 aatgaagttg gataaagcga aaatgaattc aaataaaaag gctctaagtc aaatcgtgtt
    25321 gttttcatta aatcactgaa aggaaatgct tctgcgtttc cttttttctt tttgttgtgc
    25381 gctgtatacc ttgaggatat tcactggtgg cgtacactta aatagtaata tattgtgtta
    25441 ctttatgtct gtggtaatag ttatagttgc ttaccaatgt atgttcattt ttcgaaatgt
    25501 tgacttcata atccttggct taatgtttga atttttttgg tgatagattt tttcagccgt
    25561 ataaaataaa tcgtaattct attatagaga gttgtgatta taaaaagata gcaagaaaaa
    25621 aaaagaaagt ttctatacat tttaaatatt ttattcctat ttctattgct aaaaaagtag
    25681 aaattctata tttaccttta actctcgctt ctcttgcaaa tgtagttagc attttaatcg
    25741 ataataaact caggtagtta tattaccttg aaatgattga taattttgtc acttctattt
    25801 tttttgcaat aaatttttta ttttagtgaa aaataagaaa ctatttcttt gaaggcgata
    25861 agttagaaaa atattattgt agtcttttct ttattaggaa tcatcactgt tgtatcttat
    25921 taccttttct gtttttctta ttagagaaaa ataatttgag aggtagatag tttttattgg
    25981 aggaagtaat tatgtatatt tttgaaacaa aaatttttca ttgcaataat tgcaacaaag
    26041 aaatttcatc gggggaagag gtttggacaa aaatggaaat ttccgccaaa agtaactgct
    26101 cctcaactga aatcaattaa agaactagaa tttgagaatg cactggtaat ttgtatgaca
    26161 tgtactgatg aagtatttcg aaaacatttt taaaggttga gatgtatata gatttaacca
    26221 atcatttatc tctctcggaa aaggaatatt aactaaccag cgggttatca aaaataggta
    26281 ggaaaactgt acttaatggc ttatttcgcg ctgattatga gaatatcagt ctgtttgatt
    26341 aatttttagg gttcaaaata cagagacaaa ctccaggtcg ggtttcgcca caagcgaatt
    26401 acccgatctg gagttttgtc tattgcagga aggcgtagcc ggactgcagg aataaagccc
    26461 ccggtt
//
LOCUS       DARBEG010000031        26356 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000031 DARBEG010000000
VERSION     DARBEG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.031"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..258)
                     /locus_tag="SCK20_001297"
     CDS             complement(<1..258)
                     /locus_tag="SCK20_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145376.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="SCK20_001298"
     CDS             complement(274..924)
                     /locus_tag="SCK20_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145377.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="SCK20_001299"
     CDS             complement(935..2107)
                     /locus_tag="SCK20_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG1145378.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="SCK20_001300"
     CDS             complement(2126..2998)
                     /locus_tag="SCK20_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1145379.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="SCK20_001301"
     CDS             complement(3020..3940)
                     /locus_tag="SCK20_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145380.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="SCK20_001302"
     CDS             complement(3940..4209)
                     /locus_tag="SCK20_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145381.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="SCK20_001303"
     CDS             complement(4206..4586)
                     /locus_tag="SCK20_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG1145382.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="SCK20_001304"
     CDS             complement(5053..6516)
                     /locus_tag="SCK20_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1145383.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="SCK20_001305"
     CDS             complement(6580..9597)
                     /locus_tag="SCK20_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1145384.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="SCK20_001306"
     CDS             complement(9748..10587)
                     /locus_tag="SCK20_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1145385.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="SCK20_001307"
     CDS             10732..10977
                     /locus_tag="SCK20_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145386.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="SCK20_001308"
     CDS             11040..11462
                     /locus_tag="SCK20_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145387.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="SCK20_001309"
     CDS             complement(11556..11873)
                     /locus_tag="SCK20_001309"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145388.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="SCK20_001310"
     CDS             complement(11900..12106)
                     /locus_tag="SCK20_001310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145389.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="SCK20_001311"
     CDS             complement(12087..12269)
                     /locus_tag="SCK20_001311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145390.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="SCK20_001312"
     CDS             complement(12283..12633)
                     /locus_tag="SCK20_001312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145391.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="SCK20_001313"
     CDS             complement(12659..12961)
                     /locus_tag="SCK20_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145392.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="SCK20_001314"
     CDS             complement(12964..13197)
                     /locus_tag="SCK20_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145393.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="SCK20_001315"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="SCK20_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="SCK20_001316"
     CDS             complement(14628..15743)
                     /locus_tag="SCK20_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1145394.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="SCK20_001317"
     CDS             complement(15749..16147)
                     /locus_tag="SCK20_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1145395.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17176..17562)
                     /locus_tag="SCK20_001318"
     CDS             complement(17176..17562)
                     /locus_tag="SCK20_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1145396.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17559..18080)
                     /locus_tag="SCK20_001319"
     CDS             complement(17559..18080)
                     /locus_tag="SCK20_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1145397.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="SCK20_001320"
     CDS             complement(18082..19116)
                     /gene="sppA"
                     /locus_tag="SCK20_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1145398.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19149..20564)
                     /locus_tag="SCK20_001321"
     CDS             complement(19149..20564)
                     /locus_tag="SCK20_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG1145399.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20533..22686)
                     /locus_tag="SCK20_001322"
     CDS             complement(20533..22686)
                     /locus_tag="SCK20_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG1145400.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22944..23180
                     /locus_tag="SCK20_001323"
     CDS             22944..23180
                     /locus_tag="SCK20_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG1145401.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23199..23414
                     /locus_tag="SCK20_001324"
     CDS             23199..23414
                     /locus_tag="SCK20_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG1145402.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24117..24869)
                     /locus_tag="SCK20_001325"
     CDS             complement(24117..24869)
                     /locus_tag="SCK20_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1145403.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24884..26131)
                     /locus_tag="SCK20_001326"
     CDS             complement(24884..26131)
                     /locus_tag="SCK20_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1145404.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(26207..26353)
                     /locus_tag="SCK20_001327"
     CDS             complement(26207..26353)
                     /locus_tag="SCK20_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEG1145405.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         7612 a         5173 c         3961 g         9610 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaacata
    16921 taggattaaa tctaagtact ataaaaggtt aaaaagtgaa gaatatttaa tagaaaaact
    16981 aaatttactt gctgctttcc ccttttccat taatacaaca atcagagagt gtcttttttt
    17041 cgaaaaaatt agaataaata aaagacacgt agttttttta tctacatgtc ttccatcgac
    17101 tacaagttat catttctcaa aacgtattta gcttttaatt gattaatcgt ttcacgttgt
    17161 ctacacattg cctaattaag attttcttat tagaagcatc aatcccgcaa taatacctaa
    17221 aactccagga attacccaca ataatgttaa aagaaaaaat ggaataatag ataaaatgag
    17281 taaactaata ccatagacca ctgttttttg attttttctt actaaaaaac ctattatgaa
    17341 aatcattatc gaaaaaacta gggtaattcc tccaaataaa acaacattcc cgcttagatt
    17401 gactaacata tctatatcct tactggttag ttctgtagta tttaaagtag ttgaatcaat
    17461 agaagataga aacataccac ccatcaatgc taaaagacta cataaaatcc ctgttattcc
    17521 actaattaaa atcaaaataa actctgtttt tctcttattc atttaattct ctccagatta
    17581 agtgttttag aattagaaat tttttgacca tagttaatag cttctaatga acgaataaca
    17641 accactgacg tgttagaaaa ataatcccca atatgttgtt tcttgggcgt gaaagcagcc
    17701 accaaatacc caatttgaaa tacaaaattt tttaatataa acctacatat acactcacga
    17761 ataaagatag tttgccaact tagctctttt tcctcaaagc tcactactct tatcccaaaa
    17821 atcattttcc caattgtttg tcctgaattc agcttagtca aaagtacaaa ataggttaaa
    17881 taaatagcta atgacgctat accataaaaa gtaataatgg atttgtcaga ttccagtccc
    17941 atgattttgt aaaagagacc tatagtagca tgggtgatga tgctaataca aattaaatca
    18001 ataagaaaag caaaaaatcg taaccaaaat cccgcaaata cataattagg caaagtttca
    18061 atgccactct tcttattcat tttatagccc tccatagtaa tacattggtt ttggagtatc
    18121 agaaaatcca agtttttcaa aaactgaaat tacacgatct gatgtagttg cctttagccc
    18181 ttgccattca gcaatcttat tacctaacca agaagaagca aagccagttg tgtcattttt
    18241 atattctata accgtcgcat cagttaattt cttctctttt tttagggaat ctaatgcatc
    18301 ttcagagtaa ccaatttgat ccaccaaccc attttctact gcttggttac catcataaat
    18361 tcttccatcc gcaagttctt tcactgcatt agtatccata tttcttcctt ttgctacaac
    18421 attgacaaaa cgatcataag cactcatcac aaattcctgt ataactttat tctcttcctc
    18481 tgaaggtttg tgttgaggtg acatcatatc ttttaatgca ccacttttat acgtcgcatc
    18541 agttacgcca agtttttcta ataagccaga ataatttaaa ccagaaataa tgacaccaat
    18601 tgatccagtt gttgtttctt ccgtagcaaa tattttatct gaaccagcag aaatgtagta
    18661 ccctccactt gccgctgtat ttttaaatgc agtatagata ggaatatcta gctttttaat
    18721 ttttgccatt tctttagcaa tctcagcact ttcatatatt cctcccccag gtgaatttac
    18781 ttctaataac actcccttaa ccgctttgtc ttcttgaatt ttctttagct gtgttaaaaa
    18841 attctgatgg ttatattgtt ccctactaaa aagattgctt tcacctgtat ctgcaataac
    18901 tccattgacg gatagtttga caatcttttt atttgatgcg ccttcttcta acgtttcttc
    18961 tgtgatttcg ttagatccgt aaaaaatctt atttattcct gttagggttg tatttgatgc
    19021 ttcctcttta tcttttcctg gaattagcgc gacgataatg gatagtaaca ataatccgca
    19081 cgctactccc acagcaatcc atcttctttt attcatataa actcctccta tattcttagt
    19141 aaaaataatt attttttatt tagaaacaca tcgacaattt gttgccaata tgttttttct
    19201 cctacaatta aagacaactc tccagttaat ccataacgaa acggaaacga ttgagttgtt
    19261 tttaacgttc ctttcactgc ataaaaaaca cctttctcag tggcagtaga attttctgaa
    19321 atttcatcta aagttccgtc gatcgtaatc ggagccccac ctttttgatc caatttaaaa
    19381 tgaaccttca tcccttcttt aatataggta ctttcattcg caggtaaaag tgctgtaaat
    19441 ttcatttttg atttttcgtg gatcggataa atttctgcaa gcactgttcc tttggctagt
    19501 tctgcttgtc cctttgtttc ttcatttaaa tgaaccgttc cagatacagg agcttttagt
    19561 attccttttg aaagttgatc gtccaaactt ttgataactg actggttctt ctcttgtttt
    19621 tcatttagct ctgtaactgt ttgttttaca gttgttattg cttgttctag ctcttgcttt
    19681 cctttttccg tttgactaat tctgtcattc tcagaagtag ttggtttaac taatttagct
    19741 ttttctgact gatactgttc tacttctttt tttagttgct caatttgttc atttatgctt
    19801 gctgaaattg tcagtttcac ttgatttttt tgatcatcag aaacattatt tagttgttct
    19861 tgccaattct catagtttgc catcatttct tttgaaaaat cttttaatga ttgattatta
    19921 ctccaagcta cctgtacttg ttgccattct tgtaatttac tatctgaact ctcaatttgt
    19981 ttttctagtt gtctttttgt cttttcgtat acttctaatt gcttttgatc atttaattca
    20041 ctttgcttca aagcgtagcg gagtgattct ttttctgaca acatgctctt taattgatta
    20101 ctgtagccaa aactgtcatc cgttgaaaac aaattttttc ctttacttat actttcaata
    20161 aatagctgag ccatctttat ttgttgagta atacgttggt tttcttgttc tatctgagcc
    20221 ttttcattct ctattaaaga acaatccaat ctaaccaaca catcgtcctt atgaacaaat
    20281 tgattttctt ttaggaagtt ttccattatt ttagcttcta atggcacttg taacttctct
    20341 attttttcgg ggttcaattg agccgttgtc cgaataacaa cctctttttt agcaaaggtt
    20401 aaaaacaatc caataataca taaaaaaaga attacagggt ataacaccca acggtagaag
    20461 ctatgatgtt gtttagtata gatactagaa tgttcgaacc aattattatt ctgcataaat
    20521 tgtttccctc tcctagattt cccataattt ttgatagata ccatcggcaa aacgaagttc
    20581 atcgtgcgtt cctttttcta ccaaatgccc ttcatggaga actaaaattt gatcacatgc
    20641 ttttgctatt ggtaaatgat gcgcaataaa aataatcgtt ttttctttta aattgaggag
    20701 ataattcata attgcatgtt ctaacaaggt atctaatcca ctagttgctt catctaaaat
    20761 taatatgtcc ggatttttta ataaagcccg tgctaaggct aatcgttgtc gttgccctcc
    20821 tgaaagatta tttccacctt cttccaacaa tgtctcgtaa cgtaatggct gttgattaat
    20881 aaattcattt agttggacta tctcacaaac ttctattatt tgttcgaaac ttggtgggct
    20941 atctaaaccg aatataagat tctcaatgat tgttccacta aagaaaaatg actcttgagg
    21001 caaatatgtc actcgatcgc gtaatatctg aaaaggaatg tctaagtgat taatgtttcc
    21061 ataatggatc gatccttctg atgcttcata aaaacgaaca aataactttg caagagttga
    21121 ttttccagaa ccactaactc caactatagc cgttttactg tgcgaaggaa ttgtacatga
    21181 aatttcgttc aacgttgggg ccttcatgct atacgagaat gaaacgtttt ttacagtgat
    21241 atcttgttga aataattgtt cagaaagcat atattcaaaa gactggttcg atttttctga
    21301 atcaatcgcg aaaatttcat ttaaacgatt atttgcaact tgagccgttt gcattttcac
    21361 ttgtaaatta ataatgtttt gtaaaggatc tgtcaaaaat actagtaaag cattgtatgt
    21421 gataagttga cctagactaa ttctattatc cataacaaaa taagaaccta gccataaaat
    21481 taatgcactg ctaattaatt gaatcaaatg tttaattcct tgttgaatat tatcaagcgt
    21541 aaccgtacga aatgattttt tcattaattt aacaaattca tggtctatac ggttatatac
    21601 tttctcttca ccgctatatg ctttaattgt ttctattcct ttaagacttt ctatgatact
    21661 agaatttaca gctgcaccgg ctgccatttc ttccctgttc gatttttcat agcttttcac
    21721 gaaagcgaat attgcaacta cataaaaagg caaagagcat agagtgatta aaaacaacgt
    21781 actattctga atagctaacg taaccccaac aagcaacacc ataccaatat ctaaaaatat
    21841 tgataacgta gcacttgcta aagcatcaat aatcttactg gcatcaagaa aacgagaaat
    21901 aatttcccct gattttctcg tagcaaaaaa attcattggt aaagttaaca catgtttgaa
    21961 atactgaagc ataattgcca tactcattcg ctgtcctaaa ataataagaa gataactacg
    22021 actatattca aacaataccc gaaataaata gacagcgatc aagccaatgg aaattatatt
    22081 taaagttgaa cgtgcttgat tagggatgaa aaaatcaaga ataccttgaa aataatatga
    22141 gcttcctatg ccaaaaaacg taatgaaaat cgatgctaaa acgatgtgga atactaatcc
    22201 tttttgtttc caaattatag gcaaaaaaga acttaatcca gctacttttt ctttaatagg
    22261 aatataagtt tctctaggca taggtaataa aagaatacct gtccattctt tttgaaacgc
    22321 ttcaatcgtt tttctatttt ttccattagc gggatctgca atccatagta cgtttccctt
    22381 cgttttataa attacaacgt agtgcatata gctgttatca accaatatat gtgcaattaa
    22441 tggtaagata agatctcttt ctttccagat ctcactatct gcttgaatag ccatacaatc
    22501 aaaagacaat ttttcaaatg tctttttcaa tccaaatgct gaagtaccct ctaaatcagt
    22561 cccagatagg tcccgtaatt tatgaatcgg gatatcagtt ttaaaatatt ttaaaatcat
    22621 cgaaaggcaa gcaacaccac aatctttttc atcttgttgc tgcacaaaag gaaatgattt
    22681 tatcacttat ctaacacaac tccttctaaa aaatttttat agaaaaattc tcaatatagc
    22741 tttaatacag tgaaaatatt gtctcataat tccacaaaaa ctttgaaaac agttcttgaa
    22801 cactaacttt ttcattcctg aatcaaattt attttggaaa ttttactatt caagaataaa
    22861 cttgataagt ttcaggaaga tttttactgt taatcaaata cgtatgttac agtagcaccg
    22921 taaaaaatat tatttaaaga agcgtgttta aacatgcaaa atgtaaagga actaagtatt
    22981 gtggaaatgc acaaactgtt ggtggaggca ataatgcttg gggaaaattg gggcaagttg
    23041 taggtggact taccacaggg gcagtaggtg gtgcaggttt aggaacagca atttgcggac
    23101 cagcttgcgg tgttgttgga gggttatatg gagctgtcgc tggaggtgct gcagctggct
    23161 gggatgctcg aaaaaaataa aaaaggagaa taagtagtat gcatattaaa aacacaaaaa
    23221 caacatttat attatcttca gaagagttga aaaatattca aggtgggagt gccgtgggag
    23281 ttttaggcac tactttttct ggggcaacag caggagtaaa actttgttca gccggaggac
    23341 cttatgcaat agctgcttgt ggtgtaggtg gagctttatt aggtgctgga ttcagcatgt
    23401 ggaccggtgc gtaaacaatt aaaaaatatc aaacctcaag attggtttgt gtcgattacg
    23461 aacataggta tgttctttat cttttcaaaa gtttggaatg gacagaactt tttactaaag
    23521 cttcttgtgc tagtttttat aattatatta aatttttatc ttttattaaa gtcagataaa
    23581 aattctttgg aaatgtaatg gtagagatat ttttacaaaa ggacttgcag cgctgtagtt
    23641 ggtgctgcag gaggacctat ttcggccgtt acaggaggta ttggtaatgt acttgtcgga
    23701 tgtgcattaa agcctgttcc tgcaagttaa atttataaaa acaggaaagt aattatctca
    23761 aatcctatca agtacaatca taggattgca cttgacagga caaatttttt aactaaaaaa
    23821 tgaaaaacac tttctaaaaa ttagtagaat ttgccaaatc tttcgcatct tccaccatac
    23881 atttaatttt aattcccctt tatggtactt aataaaaaca agaaaagaac tattcatttg
    23941 gccctttttt ggttccagac catagaaata ttcctccagt aactaatatt gctgttatgc
    24001 acgtaactaa taacagctat aaaaattggt acaactttaa taggattcag aaaaatttgc
    24061 actagtgaag gaatctattt tattagaaaa atgcaatctc cctaaagaaa aaaccactaa
    24121 acagtgcttt ctttcaactt ctccttaatt ttttgtgctt tccctaaagt aaagtttctg
    24181 gccaactctg aatcaaaata tagtgaacgt tttttaaaat ctatttcttt aacgttaaag
    24241 atatttacta aacacgctcg actaattctt gttaacatag gatatttctt ttcaagctca
    24301 ctaattcttc cataaaattc gtactgccca tctttagtac acaatgataa tctatgtggt
    24361 aattttgaag cttctacaaa ataaatgtca tttatatcaa aagtaaatgt ttgagaacct
    24421 atggaaaaaa caaatgcttg attcgtaacc tgttttgttg catctatcct ttcttgcgct
    24481 aataacaata actcaacaat ctcgtttcga tactcatcta gtgtttgatc ttttgttaca
    24541 aatcctaacg tttcaactct tctttttatt gttacgggta acatctcatc atgagtagtt
    24601 gtaaaaatga tttttgcttg aacatcatat tttctgatta cttctgctaa ttctataccg
    24661 ttaacttcat gatttaaatc aatatctaag aaatatattc cttgctttgg tcggaattgt
    24721 ttgaggtatt ttttaacttc taacggactt tgtgttttca atactatctt aaatacttta
    24781 tcgtgaaaaa gaatgaaatt atcaataatt cgttctaact gatttaattg tataatttga
    24841 tcttcacata atattattgg ataactcata aatcctcctt ccctcaaaac aacaaaataa
    24901 cttgagtagt aaattgatac ggatcgtttt gatagttcac aaacatattg ggattctttt
    24961 tattgatttc ctctatacta gataaaccta acccttgatg tccctttttt gttgttattc
    25021 cttttttcat tagagtatct atagagagta gtatctctct acaactattc gatatcgaaa
    25081 tttctatctg tttatcatct cggtaaatag ctagagacag ttctctatct tgactctcta
    25141 tagatgcttc aattgcatta tcgagaataa ttccaagcac cctaatacag tcaaatattg
    25201 gaacaggaac ttcaataata gtacttggac attcgaattt acaacaaatc ttattgttat
    25261 ttgcttggta tagcttcgct atcaacaaac ttttcaaata ttcatttttt atatttttta
    25321 aataacgata ttcaaactta ttggttagat aagaattaga atagatctct aattctttta
    25381 tttctttttc caatatcgaa tcatggtttt gattagcgat ttcttttaaa ctcagcaata
    25441 aatttttata gtcatgtcta aatctggcaa atttttcttg atctttttcc agtgaatcag
    25501 tatatttttt tagataaacc aattcttttc ttttcaattg ctgttcatat tgttctcgtt
    25561 gcctaatagt tattctaata aataaaaata gaacaacaaa catttgaatg attaaaaata
    25621 ttaagacacc aaacacaaaa tgatcaaata cttgatattg atgagcaaca taagaaacaa
    25681 ataaaccaat tgtaagtaaa taaatcatga atatagcagt aagtttagaa ctggactgtt
    25741 caacaatcct atttatagct aattttctat ataaccaaat aaacatcagc aaaaacaaag
    25801 aatcaagcat tatctggaat actacataac catacccttt tatcccttca aatgaaaaca
    25861 tatacgtcat aaccatggaa gtaataatag atctgaaatc atcaataagc atacataata
    25921 gtaatttatt taaaagcact atattagttt tcttcttttc ctttaatata aaataacttc
    25981 cgattatagg tatagctgcg gaaacatcag taaataattc aacaaaaaaa gtgagtggta
    26041 tcaataaaca tacaaaaaga tattttcttt tgttaagatc agtattagtc atataaaata
    26101 agagaaaaaa gaaaaagttt cccacgaaca ttcttaatag ataagctttt gtatcgatag
    26161 taatcaagag atcccctcct gattttagga tatcataaaa aaaattttag ttacactttt
    26221 taaataatga aaaaacacat tccactaaat ttgatgccgg ttttccttga ggttttgttc
    26281 cagcacctcc tttaattttt ttaagctctt gatctgaaca ttgtttagca tttagtctat
    26341 ttttttcttc cattac
//
LOCUS       DARBEG010000032        25575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000032 DARBEG010000000
VERSION     DARBEG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.032"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..1799
                     /gene="uidA"
                     /locus_tag="SCK20_001328"
     CDS             6..1799
                     /gene="uidA"
                     /locus_tag="SCK20_001328"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG1145406.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQ
                     HKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVN
                     FDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDE
                     EAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQV
                     SNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     ICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNK
                     KGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            1971..2372
                     /locus_tag="SCK20_001329"
     CDS             1971..2372
                     /locus_tag="SCK20_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1145407.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            2366..2611
                     /locus_tag="SCK20_001330"
     CDS             2366..2611
                     /locus_tag="SCK20_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145408.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(2935..3156)
                     /locus_tag="SCK20_001331"
     CDS             complement(2935..3156)
                     /locus_tag="SCK20_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145409.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(3318..3390)
                     /locus_tag="SCK20_001332"
     tRNA            complement(3318..3390)
                     /locus_tag="SCK20_001332"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Lys,seq:ctt)
     gene            3466..3696
                     /locus_tag="SCK20_001333"
     CDS             3466..3696
                     /locus_tag="SCK20_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145410.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            3740..4909
                     /locus_tag="SCK20_001334"
     CDS             3740..4909
                     /locus_tag="SCK20_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1145411.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            5483..6052
                     /locus_tag="SCK20_001335"
     CDS             5483..6052
                     /locus_tag="SCK20_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG1145412.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            6039..6899
                     /locus_tag="SCK20_001336"
     CDS             6039..6899
                     /locus_tag="SCK20_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1145413.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            7089..7793
                     /locus_tag="SCK20_001337"
     CDS             7089..7793
                     /locus_tag="SCK20_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1145414.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            7798..9633
                     /gene="walK"
                     /locus_tag="SCK20_001338"
     CDS             7798..9633
                     /gene="walK"
                     /locus_tag="SCK20_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG1145415.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            9630..10946
                     /locus_tag="SCK20_001339"
     CDS             9630..10946
                     /locus_tag="SCK20_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145416.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            10947..11816
                     /locus_tag="SCK20_001340"
     CDS             10947..11816
                     /locus_tag="SCK20_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG1145417.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            11871..12680
                     /locus_tag="SCK20_001341"
     CDS             11871..12680
                     /locus_tag="SCK20_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1145418.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(12783..13412)
                     /locus_tag="SCK20_001342"
     CDS             complement(12783..13412)
                     /locus_tag="SCK20_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG1145419.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            13582..14874
                     /locus_tag="SCK20_001343"
     CDS             13582..14874
                     /locus_tag="SCK20_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1145420.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(14950..15612)
                     /locus_tag="SCK20_001344"
     CDS             complement(14950..15612)
                     /locus_tag="SCK20_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1145421.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            15837..16121
                     /gene="groES"
                     /locus_tag="SCK20_001345"
     CDS             15837..16121
                     /gene="groES"
                     /locus_tag="SCK20_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG1145422.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            16172..17797
                     /gene="groL"
                     /locus_tag="SCK20_001346"
     CDS             16172..17797
                     /gene="groL"
                     /locus_tag="SCK20_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG1145423.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(17886..19034)
                     /locus_tag="SCK20_001347"
     CDS             complement(17886..19034)
                     /locus_tag="SCK20_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1145424.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(19136..19720)
                     /locus_tag="SCK20_001348"
     CDS             complement(19136..19720)
                     /locus_tag="SCK20_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1145425.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(19847..20278)
                     /locus_tag="SCK20_001349"
     CDS             complement(19847..20278)
                     /locus_tag="SCK20_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1145426.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(20296..20616)
                     /locus_tag="SCK20_001350"
     CDS             complement(20296..20616)
                     /locus_tag="SCK20_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1145427.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            20916..21056
                     /locus_tag="SCK20_001351"
     CDS             20916..21056
                     /locus_tag="SCK20_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145428.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            21354..21611
                     /locus_tag="SCK20_001352"
     CDS             21354..21611
                     /locus_tag="SCK20_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG1145429.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(21582..21890)
                     /locus_tag="SCK20_001353"
     CDS             complement(21582..21890)
                     /locus_tag="SCK20_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145430.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            21968..22714
                     /locus_tag="SCK20_001354"
     CDS             21968..22714
                     /locus_tag="SCK20_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG1145431.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            22729..22932
                     /locus_tag="SCK20_001355"
     CDS             22729..22932
                     /locus_tag="SCK20_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145432.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            23037..23336
                     /locus_tag="SCK20_001356"
     CDS             23037..23336
                     /locus_tag="SCK20_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145433.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            23336..23518
                     /locus_tag="SCK20_001357"
     CDS             23336..23518
                     /locus_tag="SCK20_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145434.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(23524..24000)
                     /locus_tag="SCK20_001358"
     CDS             complement(23524..24000)
                     /locus_tag="SCK20_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145435.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            24053..24778
                     /locus_tag="SCK20_001359"
     CDS             24053..24778
                     /locus_tag="SCK20_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1145436.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            24952..25293
                     /locus_tag="SCK20_001360"
     CDS             24952..25293
                     /locus_tag="SCK20_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145437.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            25286..>25575
                     /locus_tag="SCK20_001361"
     CDS             25286..>25575
                     /locus_tag="SCK20_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1145438.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         8820 a         4130 c         5057 g         7568 t
ORIGIN      
        1 ccattatgac agagacgagg caagtaattg atttaagcgg tctttggaga tttaagttag
       61 atacaaatga tgacttacaa gaagaacttg ctttagaacc aatggttgaa aaagagagtt
      121 atccaatttc agttccttct tcttataatg atttatttga aacagagaac attagaaatc
      181 atgttggatg ggtgtggtat gaaaaagaag tgattcttcc taaaaaatta ttagaagaaa
      241 gattagttct tcgttttgga tctgcaacac atgaagcaaa agtatttttg aatggaaaat
      301 tgattaatca acacaaagga gggtttacgc catttgaatc agaaattaat cagtttataa
      361 aagatggaag taacagaatt acagtggctg taaacaatgt tattgacgat agcactttac
      421 ctgtagggat tttgaaagaa acgatacagg aagatggaga cataaaaaaa gaaaatttgg
      481 ttaattttga ctttttcaat tatgcaggca ttaatagacc cgttaaattg tatacaactc
      541 cgaaagacta tataaaggat gtcactatta atacaaaaat aattgattca gatgcaaatg
      601 tagaatttaa tatcgataca gaatggaccg gagaaattct aatagcaatc tatgacgaag
      661 aagctcaact tatagcagaa gctaagggga aagaaggagt gattcaactt aagaacccaa
      721 cactttggga gcctctaaat gcttacttat atagtatgaa agttagttta attaacgaaa
      781 ataaagtgat tgatacgtac acagaagaat ttggcattcg tagtattcag gtttcgaatg
      841 ggaagttttt gattaataat aaaccttttt actttaaggg atttgggaag catgaggaca
      901 cgtatattaa tggtcgtggt ttgaatgaag cagtaaatat caaagatttt aatttaatga
      961 aatggatcgg tgctaattca tttaggactt cacattatcc ttattcagaa gaaattatgc
     1021 gcttagcaga tcgagaaggt attgtagtta ttgatgaagt acctgcagta ggattacatt
     1081 tgaatttcat ggctacttta ttagatgaca acgtagaaaa acatgatact tggaaagaaa
     1141 tacggacaga aaatgctcac aaaatggcga ttaaggaatt gatagaacgc gacaagaacc
     1201 atccgagtgt cgtaatgtgg tctattgcaa atgaaccaga ttcggattct gaaggcgcta
     1261 aagaatattt tgaaccttta gttgaattga ctaaagaact tgaccctcag aagagacctg
     1321 taacaatcgt tacttactta aagtcaacac cagatatttg taaagtagga gatattgtcg
     1381 atgttttatg tttaaatcgc tattatggtt ggtatgtttc aggtggagac ctaaaacatg
     1441 caaaatatct gttgaaaaaa gagctagatg gatggatgga aagatgtccc gatacgccaa
     1501 taatgtttac agaatatgga gcagatacag tagctggact tcatgataca gtaccaacaa
     1561 tgtttacaga agagtatcaa gtgggttatt acgaagcaaa tcacgaagta atagataaat
     1621 gtgacaattt tgttggtgaa caagtgtgga attttgcaga ttttgaaaca agtcaaggga
     1681 ttttaagagt gcaaggaaat aagaaaggaa tctttactag agatagaaag cctaaaatga
     1741 ttgctcacta tctaaagaaa cgatggtcat caataccaaa tttcagttat aaaaaataat
     1801 ttacctttta taaggaatat aaaaaaggag tggctgctta attgccaact cctttttttt
     1861 ataaaaagat tttctgttta aagaaggata tttttttcta gttttttcga aaaaaaggcg
     1921 ctttctgatt gttgtattag tggaaagggg aaaaaaggag catatttatc atgcaacaaa
     1981 attacaatga gaaatctata caacatcaat ttgatgccta ctgtaaaaaa gtattacgaa
     2041 acgaaactaa gtctattcga aaacgaaaca ttaacataac agataatgag gaaccattga
     2101 cgaattcaaa tgaaggtaag tactcccaaa ctcattttga tgaacaagat gtctatttct
     2161 tatttggaat ggaaatatta atttcagatc aaaagttatc ggcagccatt gatcatttat
     2221 cggaaataag aagaaaggtt atcctacttt attattttgc aggttttaat gatactgaaa
     2281 tcggtaaaat cctcaatatg agtacaagtg gtatctggta tcaacgaaag aaggcagttg
     2341 aacaattaaa aatggggtat gacttatggt agacaacccc aaaaaacggc agtatccatt
     2401 agttcctcta tccattatcc aatctgctct agaagaagat ccaatttcaa tcaagatgat
     2461 actcaaacat tatcatagtt atatagctaa gttatgtttg acaaatggat ttaatgaggc
     2521 ggggcaattt attacctatg tagatgagta tatgctaaga cagctagaaa ttaaattgat
     2581 agaagcgatc ttaaaattta aaattaacta actaaaaagt cccctttcca cttattctgt
     2641 ttaaaagaat cttctaatag ttaaaggatt agtagattct tttaaacaga aaacctattg
     2701 atcattgaaa tttaaataca ttctatcagt acgttaacac ttcaaggagc atattttagt
     2761 tgcgccatga tttcttactg taaagaagga gcgataaaca ttctaaaata atttttatag
     2821 tcaataaaaa gcgatgattt gaagttggga taatgatact tccgtaaggc tcgccgtaaa
     2881 cttggagaga ggtgaaattc ctatgtgctt ttgacaaaag cagctgatta agaattattt
     2941 ccatatgttg atttcaacat tttttctatt atttttaaga ccaaattcag tgaggcaaat
     3001 agcatcatgt aaatttagtt tttctattga acgttctcca ctttttagtt tttttatagt
     3061 actcaaattt aatcctgtat gtttagagat ctcatttgca gtatggtttt gtaaagtttc
     3121 tcggattatt gttatattag caatttcatt acgcataatt atgttccttt cttatcaaaa
     3181 atatcaatga ctacagtggg cttttagtga aaaaaagggg tactacagtc tcttttgaaa
     3241 acaaaaaaat acagtatata cgttttctaa atgctaagca ccaacgttta cactgtattt
     3301 aagggtttgg cacccaatgg gctgtgaggg gctcgaaccc gcgacccgat gattaagagt
     3361 catctgctct accaactgag ctaacagccc aaattaatac tttgttattc taacacctaa
     3421 aataaaaaag tgcaatactt attatcagaa ttgagagtga attttatgga ttttcaagat
     3481 tatgaaaaaa tatttgaaag ttatccatcg atattgaaaa ttactgattt aactaaaatg
     3541 ttaaatattg gaaaaaataa agcttataat ttgctgaaag atagatcaat agaaaatata
     3601 aaaattggag caaggtacaa aatacctaaa aaagctgtca ttgagtatat attgaaaata
     3661 caagatgaag gtaaaaatca tgctataata aaatagtcaa aatttttgcc gatatttcag
     3721 tttaagagga gagattgaat tgaaattaag agcaaacata attgaaaaaa gaggaacatt
     3781 acaagtagtt gtatcttata aagactctaa cggaaaaatt agacaaaagt ggaaagatac
     3841 aaaattaaaa aagaaaggaa ataaaaaacg agcagaaaaa attagagatg aatttttact
     3901 agaattggaa agtcaattag ctatggaatg tgaatgtgct aatccaaatt tattgtttta
     3961 tgactatttg cttaattatt tggaagtatc aaaaaaacaa gtagcattta acacatatgt
     4021 tgggtataag cattacgtcc ataatcgaat ttacaagttt ttttatccta aaaaaattaa
     4081 actaaatagg ttaaagcctt atcatttaca agagttttat caatatatgt taaatgataa
     4141 ctgtacagca aatacggtta ttcattatca cgcatttata cgcaaagctc ttcaagaggc
     4201 tgttattaca gaattgataa gtacaaatgt agcagataaa gtaagaaagc caaaaaagaa
     4261 acaatttgtt tctcaagttt ataatcaaca agagatcttg aaattacttg acattttgtc
     4321 agaagaaaaa ctctatttgg tagttctttt aacagcattt tatggattgc gaagaagtga
     4381 agttttagga ttaaaatggt ctgctattga ttttttaaac aaaaaaatca tgataaacca
     4441 tgtaatcata gaaaatccag agaatatgtc acagttaata aagaaagatc aaacaaagaa
     4501 ctcttccagc tatcgtacat tacctttggt tgaaacgatt gaacgagcat tagtaaatca
     4561 agctaaatgg caacaagata atcgtattct tttaggagat gattatattt tagaagataa
     4621 tgagtacgta tttactatgg aagatggacg gttgatgaag cctcaatatg taacgcatcg
     4681 tttgagtaag ttgattaaga aaaatggatt gaaaaaaatt cgctttcatg acttgcgtca
     4741 ttctaatgct tcaataatgt tagatagtgg ccagaatatg aaatccattc aagaatggct
     4801 gggacatgca tcgtattcga caactgctaa tttatacacg catttaaccg caggagtaaa
     4861 agatcgtgca gcagaagcat tagaaaacgc atttgggttt aaagaataaa aaaacaaaaa
     4921 acaatatctt tggggtatcg cttcttgttt ttagaatttt tttgttggca atttgttggc
     4981 agtatgttgg caatgaaaaa attcacaaac taaaaaccta tttataaagc tttataaaac
     5041 ttttttcaca aatagagcat ttggttaaga gtcagctgct ctaccaactg agctaacggc
     5101 ccgtacaaaa agtacttaat aatagtagca ctaagaattt taaaatgcaa gactacattt
     5161 aaagaaaatc agaaaaatct ttacttcctt ataggaaatc attcatagat tattcacatt
     5221 tgctcgctat tatataagca taccaacaaa caaagttctt gcaagagaac aaaccctaac
     5281 acttttttca ttttttactc ctttcccgcc gtctccccgg cgggtctcct gttcttaacg
     5341 ataaaagaca agaaggctta gccttcttgt tttttgtttt catggaatcc cgctaatcaa
     5401 atcacctagt taaagcaact agattgaatg tttatcataa gtctgatatc atgtttatga
     5461 aatgaaaaaa ggagcggtta tgatggaaaa aatgcgtcag caattaatgg agtgggggaa
     5521 ggaactagaa acattccgtc tgcctcactg ggaagaactc cctgacttag aactttatat
     5581 ggaccaagtc agaacgttag ttgatcgtta tctttcacct gtgatacaag gagaaaagca
     5641 tccattgctt acctcttcta tggtaaacaa ttacgttaaa ctaggtttga taccagcacc
     5701 agttaaaaaa agatataaca aagaacacgt agcattttta cttgcaatca ctacgttgaa
     5761 acaagtattg actattcctg aaatcaaaga agggatattg ttccaaggaa aaacagtagg
     5821 aattcgtgaa gcctataatc tattttgcga tgaacaagaa gcagctgtgt ggatggtcag
     5881 ccaattagca caaggtaaat ctcatccgca aaaattcgac caggcaaccc ctgtagaata
     5941 tattgctgta agagcggcaa ctttatcgtt tgcaatgaaa cttcttgcgg aaaaaacgat
     6001 tgttctagaa accgaatatt taaaagagga gaaaacaaat gaaaaacaat aaaatcgcaa
     6061 ttttagtaga ttcaggtacg gatgttccag aagaattgat aaaaaaatac gagatgtata
     6121 tgatcccatt gaaaattatt tataaagatc aggtatatac agataaaatt gacattaccc
     6181 ctgaagagat ctatcaacgt ttgccagagg aaatccctgg tacatctttg ccggatggtg
     6241 agtcgatcac agaaatcttt gagcaaatca aaaaagacgg ttatgaaaag gtacttgcgg
     6301 taacaatttc cagtggattg agcgggacat ataatgtggt tcgtttaatt gcacaacagc
     6361 aagaagaatt ggaagcatat gtactagata cgaaaaatat tggtattggt gcaggttttt
     6421 ctgcgatcca agcagcaaaa tggctggaag aaggagtaga atgggatcaa ctgatcagta
     6481 atctgaatga gttagtcgaa agaacaaaag ttttctttaa tgttgcgaca ttggaatatc
     6541 ttcaaaaagg tggacggatc ggtttagttg cttctattgt aggaaccgct ttaaagctta
     6601 acccaatcat ctcatgtaat gaagaaggga tttattacac agtaggaaaa gctaggggac
     6661 gtaaaaaaag tttggataaa acggttgctt acgtcaaaga aaaaatcggt aatgcaaagg
     6721 tcttcaactt agctgttgcc catggggatg cgaaagaaga agcaattgaa atggaagcga
     6781 gacttaaaag agagttccca caagcaaacc aaatctactt tggccagatt tctccggcat
     6841 tagtagtcca cactggtccg ggattattgg gtgtcggtgt acaagtttta gaaggataaa
     6901 tcaaaaaaag gaaacactga ggtgaaaatc agtgttttcc ttttttattg aagttagaac
     6961 aaaaaataat aaagtatttt gcactttcat tttaattttc tcttttgttt tataggcaaa
     7021 gatgaaacaa aagcagaaat acggtataat gagaaggtat gacaataata tataagaggt
     7081 ggctctttat gaaaaaagtt ttagttgtcg atgatgagaa gccgatttcg gatatcgtca
     7141 aattcaattt agccaaagaa ggctatgacg tttatactgc atatgatggg gaagaagcct
     7201 tggaaaaagt tgctgaggta gaacctgatt tgattttact tgatttgatg ctacctaaaa
     7261 tggacggctt agaagttgct cgtgaggtac gcaaaactta tgatatgccg atcattatgg
     7321 taacagccaa agattctgaa attgataaag tcctaggctt agagttaggc gccgatgatt
     7381 acgtgaccaa gccattttcc aaccgagaat tagttgcacg tgtcaaagca aatcttcgtc
     7441 gcggtgccac ggctgcgaag gaaccagaag aggcagcacc tgcggaattg acgattggtg
     7501 atttgacgat ccatccagaa gcttacatgg tgacgaaacg aggagaaaca atcgagttaa
     7561 cacatcgtga attcgaacta ctcttttact tggcgaagca ccttggacaa gtgatgacaa
     7621 gagaacattt attacaaacg gtttggggtt atgattattt tggagatgta cgaacagtgg
     7681 atgtaactgt acgtcgtttg cgtgaaaaga tcgaagataa tccaagccat ccaaattatt
     7741 tagtgacacg tcgtggtgtc ggttactatt tgcgtaaccc agaacaggag taattggatg
     7801 aaaagaaaag tccgcttttt tcgatcagtc aattttaaga tcgctattac ctttattctg
     7861 attttattga tctcgatcga aattattggt gcctacttta tacgtggttt agaacgttcc
     7921 acgatcaata catttattaa agatatgaat caaacggtgg aatcattagc aacaacaatc
     7981 agcccggaat tgaatcgaaa agataatgca gatgatgaag aggtaaatgc gaatatcaaa
     8041 agatttattg aaaacagtgc gactagcgat atcatcgaaa tacgggtcgt tgacgaaaaa
     8101 gggatcatcc gaggaacaac cgatgtcaat gaacaaagcg ctgtgggaaa gaaaaatgat
     8161 tatgttgata tcaatgattt cacgactaaa cgttatgttg ctttagataa cgacgataaa
     8221 cgggtgaata tcaatgttca accgatcctg tccccgaccg gcgatgccgt tatcggggct
     8281 ctttacgtca aaagtaatat cgaacaaaaa tattcggaaa tcaataacac agcagtcatt
     8341 ttctttactg cctcactaat tgcggcattt atttcaatga tcgtatctgt tttagttgct
     8401 cgttcgatta cacagccgat cggtgaaatg agagaacaag ccattcgaat cgctagagga
     8461 gactacagtc ggaaagtcaa agtgcatgga caagatgaac ttggacagct ggctgatacc
     8521 ttcaatcagt tagctgaacg tattgaagaa gctcaagata caatggaagc tgaacgcaat
     8581 cgtttggata gcgtgctatc ccatatgaca gacggcgtca ttgcgacaga tcgcaggggt
     8641 aaagtcatta cgatcaatga tatggctgtt tccttgctag atatcaaaaa cgaggaagca
     8701 atcggacaat cgattttgac acttttagat atcgaagaag aatatacgtt gcggaaatta
     8761 ttagaagatc caaacgaaat ggtacttgac cgttcaacca gtacattgga aagtgatcag
     8821 atgatcttgc gagtagactt ttctatgatc cgtcgcgaat caggttttat cagcggatta
     8881 gtagctgtct tgcatgatgt gacagaacaa gaaaaaacag aacgggaacg tcgtgaattc
     8941 gtctctaatg tttcccatga acttcgtaca ccgttgacaa gtatgcgtag ttatatcgaa
     9001 gcattgagcg aaggtgcgtg gaaagacgag gaagtagcac caaatttttt gaaggtaacg
     9061 ctagatgaaa cagaccgaat gattcgaatg atcaatgacc tcttaaatct atctcgcatg
     9121 gatacaggta atacccagct tcaattagaa tatgtcaatt tcaacgagtt agtgaatttt
     9181 gttcttgacc ggtttgatat gatggtggga aatcaggaaa aaaattataa gatccgtcgt
     9241 gaatttacgc aaagagatct ttgggtcgaa gtagatacag ataaaatcat ccaagtagtc
     9301 gataatatca tgaataacgc gatcaagtat tcaccagatg ggggaacgat tacttgtcgc
     9361 ttattagaaa cgcataataa cgtgatttta agtatcaccg atcaaggatt ggggattcct
     9421 aaaaaagatc tgaaccgagt gttcgagcgg ttctatcgag tagataaagc acgtgcaaga
     9481 gcacaaggtg ggacaggact tggattagct atttctagag aagtgattaa ggcgcatcga
     9541 ggcgctattt gggcagagag caaggaagga aaagggtcga ctttttatat ctcgctgcca
     9601 tatgaacctt atgaggagga atggtgggaa tgagaatctc tgaaaaagtc gttcgtatag
     9661 gactcatttt gatggttgcg ttaagcatat acttctctta tgcaatctgg ctgagcccag
     9721 caggaaaaag ttcaatcaac gtagatgaga caaacactca aatgatcgag tctcaaagca
     9781 atcgaaaaac ttccgaagtc tttcttccgc ttcatgttac atggcaccat tcgggaatga
     9841 tccaagaaac caacagtgaa aatctcgtgt accgattgca attgatcatc gataaagcaa
     9901 gatttggaaa gctttctgaa gtagtttcag gagacaaatc acagtttgaa caggtgaaaa
     9961 atctgaactc tggttttgag ttggcctata atgcgccttt tttgctaaga gaatacaagg
    10021 aagcatttga tttagattta gatgtgtcga acttggcaaa agaaagcgaa aatatgtatt
    10081 ttactagaat acaattcgat caaggaaata aaaaaatccg atttttgaat tattcgaacc
    10141 atctcgtata tgaagcaaat atttcgttga attggacaga tatcaataaa gaattaacag
    10201 caaaagacac aaaatggaca gaaatgacaa cagaaccatc gacgatcgac acgcaatatg
    10261 ctacgaaaca ttcgatcaaa ctgaagaagt atagttatat cctgtcacag caaccctata
    10321 cactttttag aaatgctttt ttccagaaac cagatagcgt gaaaaataat gatgaaagca
    10381 atgaattgat tttttacggt gggaatgaaa caatgtctat ccattcggaa acgcaagtag
    10441 ttgattttcg tggagagctg ccagagaaaa aagaaaatgc tagtgtattt tctgaaagtt
    10501 tcccctatat tagcaaatta ggcagttctt tagggaacct cagatatttt gaccgcagtg
    10561 acaatacgat cgattatcgg atattcgtgg aaggcttccc ggtttttggg acagacagca
    10621 gaggacagat tggtgtggaa gttggcggtg aaacgaatgg ttctgtgcaa gtgaatatcc
    10681 aaaccagttt gaatacgatt caagtgccga taccttcaga cgaagaagta gaacttgcaa
    10741 gtacgaatga aatcatccag cagttaaaag ctaagggagc agatagcaac aagattggtt
    10801 cagtgatcgt tggctataca tggcaaaatg tcaaagaaac gaatcgtgtc gttgatctct
    10861 tacctgaatg gtatgtgaaa tataaagata actggtattc agctaacgac cttctggaaa
    10921 aattacctgg attggaggcg aattaattgg atttcaaaaa aatcgaatgg atatttttcg
    10981 tcgctttttt agggttgaat gtgtttttat tcagcagtta tcatgacgcg gtataccaag
    11041 aaaacaatgt cattcgctct aatcagactg aatcaatcca acaacgtctg gctagcgacg
    11101 atatcactta cacggggaag atttcgtctg agaacaagca aggctattat cttagcgcgg
    11161 agcagaccaa tcttagcact tcattagcga attatcgcaa ggctggtaaa agcggcttgc
    11221 tggcaagtgg tgtgttttta gacggtgatg ttttgacgaa atctttatcc agttcagatg
    11281 ctgaaaagaa cgtgatcgat ccagataaaa ttagtagttc cttggaagat tttttatcta
    11341 acaaagacaa tattttattc ggtaatgatt atgtgtacac gaaaaacttt tctcatttag
    11401 aagaagaacc actagaaatc caagcaagcc aagagtatga aggcatccca ttcaatgatg
    11461 atacagcaaa aatcgttctt tctttagaaa aaaacgataa tgattatagc atcacgaaat
    11521 acatgcagac gcatttatct gatatcgagc agctgcgcga aaagacggaa ctgtatacgg
    11581 aagaggaagc aatcaataca ttgtacatca ataacaaaat accaagagga tcaaaaattt
    11641 tatggcgtca gctagcttat tcctgtattc tgaaagtccg ggaaaaaaat gtgtatgttc
    11701 ctgtttggca tgtagcgatc gaaacaagcg ataaagtggt ccaaattgag agcgtcaacg
    11761 ctttcagcaa tacgatcgtg acaaacaata ctgtaccaaa agtggaagat caataaaaaa
    11821 gcgagtataa tgaaaaaagt ttacaaagta agaagggaag taggagtcaa atggctgaag
    11881 aaaaaccagc ttttaaaatc agtatcctag caagtggaag ttcgggtaat tcactttata
    11941 tcgaaagtga aaaaaaacgg cttttggtcg atgcaggact tagcggtaaa aaaatcactt
    12001 ccttgatggc tcaaattgat cggacaccgg accaattaga tggaatactt gtgacgcatg
    12061 aacatcgaga tcatatacat ggcgtgggtg tcttggctcg taagtatcat ttggatgttt
    12121 ttgccaatca gcagacttgg gaagcgatgg aaccactgat tggaaatgta ccagtggaac
    12181 aaaaacatct atttgagatg gggaaagtca gaacattcgg cgatatcgat attgagagtt
    12241 tcggcgtctc ccatgacgca gcggctccgc aattttatcg tttccataaa gaaaatcggt
    12301 cattcgtcat gttgacggac accggatatt gcagtgatta tatgcgaggt gtcattgaaa
    12361 atgccgatgg ttatttgatg gaaagtaatc atgatcttga gatgttacgc atgggacctt
    12421 atccgtggaa tttaaaacag cgtattttag gagatagagg acatctttcc aacgaagacg
    12481 gtgcacttgt catgactgat gtgatcggta atcggaccaa acgaatctat cttggccatc
    12541 ttagcaagga aaacaatatg aaagaacttg ctcatttaac aatggaaaat gttttgaaag
    12601 aaaaagattt tggcgttgga catgattttg aaatctatga tacagatcca gatgaagcaa
    12661 ctgagctatt ttcgatttaa acagttcaat caataaaaat ataaaaagca gaagataaaa
    12721 tcaatcaggg ttggttttat cttctatttt tattttttta gcatattaaa aaaataaccg
    12781 tattatcgac tgactacact gatgtgctgc ttttcatgtt tctcatccag tatctcatct
    12841 actaatgcaa tcgcgtaatc tgccatgctg atcgtactgt cgctagcagc gtttctttgt
    12901 aaataatcat cacttaacaa ataagtacct gtacgtttgc catgtggatc aaataaagaa
    12961 gctggactga tataagtcca atgtagattt ttagattgtt tcaattttaa caatccttcg
    13021 tacatattga aagctgttgg atagaagggt gcatttttag gtacttggtc aatcatacga
    13081 gtcgtctttt ctgtatctaa gaataacgaa gaggcacccc caacgacaat taagcgtgta
    13141 tcggttcctt caagcatctc aatcaaatgg gacaaagaag tttgatgcat ttcttctttt
    13201 cctttgggag cgttgaaagc atcaacgaca acatcaaaag atgcaagatc agtagttgtc
    13261 aactcaaata aatccttttg tataaacgga acatctgatg gcaatttttc tggatgacgc
    13321 acgattgccg tcacgtctaa atctctggac aatgcttcgt ctaaaataaa tgaacctgca
    13381 tggccagtag ctccaatgag tgcaattttc ataaaaaacc tcctttttta ttcctgtcca
    13441 ttgtaagcag ttgctgaata ttcgtcaaag aatccgattt ttgaaaagct cagtgcgcat
    13501 tcctttcatg gttcgtgatc attgggtata gttaaattaa gaaaactgat tgataaaaat
    13561 agaagttgaa ggagataaga aatgaaagag tggttagatc gtgccttttt accaatgata
    13621 acatctgttg tgaatcttag acccttaact gctttgcgta atggaatgac ttacacgatc
    13681 ccaataatta ttgttggctc gctgtttttc ttatttgctc attttcctgt ggattacatc
    13741 gctcagtgga tagacaaaac aggattttct atgtatttta cacaagtata ccaggctaca
    13801 tttgatgtga tagccttttg gatcgttttt gccattgcat acgcttatgc aaaagaagaa
    13861 gacatcgatc gcttaccagt cggcataaca gccttgtctg cttatctact gcttattcag
    13921 ccactaaata ctcagataga tactgtctgg acaggtgctg aagggatact aggagcagtt
    13981 acaactgggc tcatcatcgg atggggctat gcacgtttag taagacttgc gaaagggtgg
    14041 aagcaaagag agaagatacc tgaaaatgtt tggcagtctt ttacttcatt gattccaatc
    14101 attgttatag tcacttttac gcttatcggt tcagcggttt ttcaacattt gttcagaatg
    14161 actgttgttc aaggagtatg gacatatgtc cagcttccca ttcaatcgtt catagacacg
    14221 ttgtttggtg tgcttttatt aggttttttt gttccgtttt tatggttatt tggtcttcat
    14281 ggatctgcga tggtcaatgg gttggttagt cccgtcttgc aagcgaattc attagcaaat
    14341 gcagaaatct tagcctcagg acaagagttg acggtggcaa atggcggaca tatcgttact
    14401 cagcaatttc tcgatcagtt catgacggtt acgggtgctg gattgacgtt aggggctgta
    14461 ttttttatga cggtttttgc gaaatcgaag aaatacagag aattaggaaa tcttgctctt
    14521 ttaccagcct ttttcaacat caatgaatca attatttttt ccacaccgat cgtgatgaac
    14581 ccgatgatgg ctgttccttt tattttcgca ccggtcgttt caggattgat tacctattcc
    14641 gctttatatt ttggattcgt tcctttattt acagcagtcc aagtcccttg gacaacaccg
    14701 ccgatcctct cgggtttctt agtaggcgga gtgcctgcag cgattttaca aggtatcgtg
    14761 ttgtcaatta gttttttcat ttatttccca tttttgaaaa aaatagattc tgcaaactgc
    14821 aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa agatgaacga ctaaaaaaca
    14881 aaaatactcc tgtcgcttta gattgggaaa atcaccaatc taggatgaca ggagtattta
    14941 tagtcatgtt tatactagcc ctttttggac agctaattga acaagaacaa ctaatgtgtt
    15001 cattcctacg tgtgtgatca tcgatgtcca gatccgacca gttgctttgt actctaggct
    15061 gaagaagaaa cctaggaaaa agtagattag cagatgacca tcattatgag caaacgcaaa
    15121 cagcaaagag ctgatcactg ctccgaccca aaaatttgaa tatcgactga tgatccccag
    15181 tatactgcgg cggaatacga actcttccat gatgggtcca gcaacagttg tcgccaaaat
    15241 aaaagcgggt gcttccatga tcatctggat gatattttgt gtattctcgg aaggggttgc
    15301 tttgccaaac aatacagatt ctaacatgac agcgatgctt tgcagaatca atgcgacaaa
    15361 gattccaacc aatccataaa taacaatctt tggagcagaa gcttttttta ccgctgattc
    15421 gatcgctaag ggttcacgtt gcttaaaata aaaaatagct aggacaacgg cgcctagtat
    15481 atacatgact gcagttgctg tgatcaattc agacgtagat tttacaagtc ctatcgaagc
    15541 taggatcaag ggtgagataa aaattagacc atagcaaaaa atgctgagga agctatattt
    15601 ttttaaagac atgtaattcc tccttttaag ggataacaag catgcaaggc aagcttatat
    15661 cgattgattt caattatatc ataatttcaa taagcagctg tcagaataaa atttcttttc
    15721 tttgatttaa aagcttgcaa aaaagaggga gaatgagtat tattaaaaat gtaggttagc
    15781 actcgaaggt taagagtgct aacagattaa aaaaagagtt atcggaggga tttatcgtgt
    15841 taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa agaagaagaa aaaactgtcg
    15901 gaggaatcgt tttagcttct gctgctaaag agaaacccca aactgggaca gttgttgcag
    15961 tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc tgccgctgta aaagcaggcg
    16021 accaagttat gttcgaaaaa tacgccggta cagaagtaaa atacgaagga aaagaatatc
    16081 tgatcgtcgc aggaaaagat atcatggcga ttgttgagta aatttggaag ataaaaaaat
    16141 aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa gaattgaaat ttgcagaaga
    16201 cgcacgcgca gctatgttac gcggggtaga taaattagca gatacagtga aagtaacatt
    16261 aggacctaaa ggccgaaatg tcgtactaga aaaatcttac ggctctccat tgatcacaaa
    16321 tgatggtgtg acgatcgcaa aagaaatcga attagaagat cattttgaaa acatgggtgc
    16381 aaaacttgta tcagaagtag catcaaaaac aaatgatatt gcaggagacg gtacaacgac
    16441 agcaactgtt ctgacacaag caatcgttcg cgaaggttta aaaaacgtaa ctgcaggtgc
    16501 taatcctcta ggcattcgtc gcgggatcga attggcaaca aaagcagcag tcgaagaatt
    16561 gcataatatc tcaactgttg ttgattcaaa agaagccatt gcacaagtag cagctgtttc
    16621 ttcgggttct gataaagttg gacatttgat tgctgatgca atggaaaaag ttggaaacga
    16681 cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca gaactagatg ttgtagaagg
    16741 tatgcagttt gatcgtggtt acttatcaca atatatggta acagataacg acaaaatgga
    16801 agctgttcta gaaaacccat atatcttgat cacagacaaa aaaatctcta acatccaaga
    16861 tattctgcca ttgttagaac aaatcttgca acaatcacgt ccattgttga ttattgctga
    16921 cgatgtagat ggtgaagcat tgccaactct tgtattgaac aaaatccgtg gaacattcaa
    16981 cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt aaagcaatgc ttgaagatat
    17041 cgctattttg acaggtggta cagtaattac tgacgatcta ggtttagaat tgaaagatgc
    17101 aacaatcgaa aacttaggaa atgcttctaa agttgttgta gataaagaca atacaacgat
    17161 cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt gtgcaattga tcaaaaacca
    17221 aattgctgaa acaacttctg attttgaccg cgaaaaatta caagaacgct tagctaaatt
    17281 agcaggtggt gtagctgttg ttaaagttgg tgcagctact gaaacagaat tgaaagaatt
    17341 aaaattacgc attgaagatg cattgaatgc tacacgtgcc gcagtagaag aaggaatggt
    17401 ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa gtttctgctg tagaagcaga
    17461 aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct ttagaagaac caattcgtca
    17521 aatcgctgaa aacgctggtt atgaaggatc agtgattgtt gataaattga agaacgtgga
    17581 actaggcact ggtttcaatg ctgctactgg agaatgggta aacatggttg aagcaggtat
    17641 cgtagaccca actaaagtaa cacgttctgc cttgcaaaat gccgcttccg tttctgcctt
    17701 gttattaacg acagaagcag tcgttgcaga taaaccagaa ccagcagcgc cagcagctcc
    17761 tgcaatggat ccatcaatga tgggcggtat gatgtagtaa ttaagaaaga accctatttt
    17821 aacagggttc tttcttcttt ttttgtttta ttttaagcaa aaggggcaaa gaaggggcaa
    17881 aaatatcaaa ggctatttag tttatcaatt acttgttgtt tggctctttt tgttacatgg
    17941 ttataaatag acagagttgt gtttgcatca gaatgcccta cacgctccat aatggcttta
    18001 agaggtacgc ctaattcaga taatagtgaa acgtggctat gtctgaatat atgtgaggat
    18061 agactttttt ctaattccaa ctcttcctct actttatgga gtatagcgtt aaatgagtgc
    18121 agcgcaagcg gagtgccact tgtagatata aatatatatt gatcgggatc tgtgggtcta
    18181 cctgcaagaa tattgtcagc tatcacactt tcaatcaatt cttttgcacg attgggtaat
    18241 tgcacttcac gttgcgaata agtatttttt ggagttgttt ttatagcatt atccattttc
    18301 acagatgtgt aatctaaggt cccattaatg gaaattttcc catcctcata gtccttcatt
    18361 tgcaaagcta gcaattcccc atatctcaaa ccagttaaat atagaaactc agctattatg
    18421 ccgtgtagtt ttctgcgagg attggagtat agctgtttca gtatttgatc aatttcttct
    18481 ttctccagat attttttatt catagaaagc cttctttttt cttcttccac ttttttagga
    18541 tggattttaa ctgctagcgc agggtttctt tgaatgtatt ttctatcgat tgcatagttt
    18601 agcataacag atagagttgt ttttgtttgt tttgtgtagt tcagtgagag gtcaccaaac
    18661 gtatacatat cttcaattat cttattaatg agtgtctcat caatgtttct aacgatcgta
    18721 tcatcgctta tgtgcttaga aacatgtttc atcatcattg gaaccttcaa atagctagta
    18781 cgtttaacat gctgcttata atattcatac cattctttat acagctcacc aaaagtgata
    18841 tctgatttat tgtaatcttc gagtgcttct ttaatttttt tatctagaat tttctgagct
    18901 tttttccacg cttgtggtga attacttgta agtgttgtag atttttttcg tgttttttct
    18961 gtataaggat ctacatatct ttcaataaac ttgaattggc catctttggt ctgttcaacc
    19021 cacactttta acatctccta tcatttgcta taataggcat aacaaataga cctatatagg
    19081 tttgttttct aaaagcacgc tcttactctg gacggtgggg cgtgtttttt atttattata
    19141 cagttgcttt aaaaatagct gctttttcaa atgatactaa aggagagaag tggatttcta
    19201 tttctccctt ttggtttaac gtgaaatgag cagttacatc catttgtttt cctggagcaa
    19261 cagatcccat tgtattttcg ttggcatatg tctctgattt tttgtcatct ggtccatata
    19321 cttccacatc tgtacctaca gggatatctg aatcgccatc attttttacg gtataagtaa
    19381 tttttactac ttgtgcaggt tggttttctt caaattgatt tctttcatca gttagttcta
    19441 cactatttag tgtatattca gcatcaccaa cttttacagt gtcaccaatc ttatagaagg
    19501 tatcgctttt ttcttcttta gaagatgaag cagaggttga agatttagtt actttttcgc
    19561 caccattatc acttgcttta ttactatctg atccaccatt taaagcagaa ccaataatta
    19621 taattaaaat tactgctaat acccaaaacc atactttttt gtaaaaaggt ttactaactt
    19681 tatacatttt tccgtcttga cccataactt tttttgccat ttaaatattc ctcacttctt
    19741 gttataatat atttgcgatc tcagaaatga ggtatgagtc cgtgtgccag cacgggcttt
    19801 tttaatgttt tgctgaaatc ggttttttta ataattcttg atgtattcat acatattgcc
    19861 ttgcgtaaga atattttttc ttaaaatagc attggcagac agcataacaa gtgtatctga
    19921 gcttattctg aatagaataa tactccataa atttttcgag attaaactga gattcatcag
    19981 tcagttcgtt ctcaatatag atatttaata aaattagaat agctatttta tctgcttctg
    20041 tttcaaattt tgagtgaaaa gttgtagaag tatcgtacaa aactgaatat tcaaaatgag
    20101 aagcggtgaa atgcgcaagt tcatgagata aatgaaaggc ttctgcagtt tcgccatata
    20161 gattttcatt caaaaaaatg attctaggct tcggataata gaagccaggc tctttcattt
    20221 ccatgtaaac tactttcaaa ttgtattcgc tcaacatttc tttcaatttc aaatacatac
    20281 aacccatcac tccaactatt cattttcctc taaagcttta gcaattgcaa tcgctttacg
    20341 cattgtctcc ttagatattt cttttccatc aaaagaaaaa acagtatcgt tttctgataa
    20401 atccacatgt ttaggggttt ctctttcttc tctacctaga aggtagtcta cagagactcc
    20461 aaagtaatca gctaaccgag ctaactcaat agaattagga gtttgtcttt tccaacttcc
    20521 taaatatcca ttcgaatacc cgaaattcat ttcgagctgt ctaatggata atcctttttc
    20581 ttttgctaac tcctttatta tttcgtaagt attcattgat aaatcaacct ttctgaatgc
    20641 ttacaaaaaa agtttagata aataatgtaa aaatagttga cttatataca ttattaatct
    20701 atactatgtt ttgtaaacaa gtttaacaac taaaaagaca acaaaaaata ctattgatta
    20761 ataaatgcca accgccaaga aagcttttta aatcaaagtt tatatgtctt atttaactat
    20821 gcatttagta tagattatta atctaataaa gtcaacgcag ttttttaaaa agttgttaaa
    20881 tttgtttacg aatataaaag aaaggagaga agaatatgga aaaaacagtc tcggcaaaaa
    20941 tcgaagattt gaaattagac atactgaaac aagcaaaagt ggcgatggaa cacgcggtag
    21001 ataaagaaga ctctgccatg gttgcagcca tagcagagat tttagctcac gtttagtcat
    21061 cgtttttacg gtcttctgga agctgaccat agataacagc ataatgaata tctaaataag
    21121 cttgaacaat ttcttttggc gaaattgatt cgcctttagt agatacttgt gattcgtgat
    21181 ttagccatgc aacaactaaa tccgcagcga tttcaaacgg cgttacactt tgtgtcagtt
    21241 gccataaaga atttcacaat atgtatggtc gtggtaataa taccaaagag caatttgagg
    21301 aatttatgga aattaaaaaa tacaaaaaat catttattaa aggaggctaa acaatgaaca
    21361 aggaaatgag ccaaacaatc aaggtacaaa aaatgataga tgatctaaca catggaattg
    21421 atagccaagc cgataaaatc atcaaggaat tgcaaggaca aaaagttaag gatgcaaaaa
    21481 tgcttctcaa gactatcaac tttgaaatga atccaacaaa aagaaagctc gccgatgtgt
    21541 tggaagaaaa gttggcttcc gctatcaacg agcaagaatt actatttgaa accgatactt
    21601 ttaacagctg atgttttata gatgacagtt tcatccgaat ctatatcatc gccgagagaa
    21661 aagaatgttg cttttgacaa aaagccgata agacctacaa gcggtcttga tgttgcaaga
    21721 gtagataccc caatatcacc ttgcaaggta ttcacgtaaa tttgagaaga atagaacatt
    21781 ccatcttttt cttcgatatt tgacgcatca tcgtatccaa cgaccaaagt atcggcaaat
    21841 attttgattt tttctccatc aagcatagta attacaaatt ctggtttcat aaattttcac
    21901 ctccttaaca attatttcag cctgtcacac tgataaggaa attataccaa agaaaggaat
    21961 gagaaatatg aatacaccac aaatttttaa tttcgagcaa aacgaagttc gaactatttt
    22021 agtaaatgat gaaccatatt ttgtaggaaa agatgtagca gacgttctag gatattcgaa
    22081 tccgcaaaaa gctattcgag atcatgtgga cttggaggat aagacgcaga acgattcgtt
    22141 caccgtcaat ggaacagcag ttgttttgat taacgagtca ggcctttaca gtttaatctt
    22201 aaaatcaaaa cttccctctg ccaaaaaatt taaacgttgg gtaacgagtg aagtccttcc
    22261 aacaattaga aaaacaggta gctattcaaa cgtacctcaa agttttgcac aagcattgcg
    22321 tttagcagcg gatttagaag aaaagaacca attactcgaa caacaaattg ccgagtacga
    22381 accaaagatt agctacttag atacgattct ttcatcgaca gatacggtag ctacttctca
    22441 aattgcagct gattacggaa tgtcggcaat tgctctaaac aaattgctaa acgagttagg
    22501 tgttcagcat aaagttagcg gacaatggat actttaccga aaacatatga accaaggata
    22561 cacaaaatcg cacacaagtg agataccgaa agccgatggc ggcactaaag ttgtaatgaa
    22621 taccaaatgg acacagaaag ggcgagtgtt tatttacaac ttattaatcg cagagggcta
    22681 ttaccctcaa atggatttag aggaaattgg ttagaaagga gttttagtat gactgacatt
    22741 gcagaaatca ctcaacgaga tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc
    22801 ttaacttaca ccatgcttgc tgaaagattt ggaattagca aaagctactt atctttaatt
    22861 ttaaacggta aaaagacttc tgcagaagca aacagaatta tagattcgat tatcactatg
    22921 tacgaattgt agagggaggt aaagtcgttg gaagataaaa tcattcttac aaaatcagag
    22981 cttcaagaaa tgttaaacat cgagtatgga acgataattt ttaggaggga taataagtga
    23041 aaaaaccaac actttcagag ctgatagaag ctgctgagaa ggcagtaaaa ccagacgact
    23101 ggtaccgaca aagtttaatc ttggagaagt tccacggcat gtcaaaaact actttagttg
    23161 aatactgcaa ggaaatggaa acaattcctg aatttgcaga aggaatactt cgtccaggtc
    23221 actcaaccac atttattcat taccatactt ttatttggtt tttaaagtgg aaagacgcaa
    23281 acaaatatcg tgtaaaaagt ttatcgcctt cagatgtttt gaaggaggca agttaatggg
    23341 caaattcaac agagcactag tattcagcgc accgctaatc atctacgctt taggcctctg
    23401 gggaagcaga caagcgttga tagggacaat cgtttacatg gtttggattt ttatggggct
    23461 tgatgaagct gagcacagag cgaaaaaagc cagtcgggag ggactgacta aaatatgatt
    23521 tttttatttt tgttcctttg ggctgctatt gcgtacttca tccagctcct tgttgtcaaa
    23581 gttgctctta tttcttttag gaagaacaaa agaaaatagc gctataaatg aaagataagt
    23641 agccgctaca ttaaactgtt cttttgaaaa agttacagtc atcggaatga taattgatat
    23701 tgaggaagta atcttaatta aaaatatagc aatattttca acatcgcgtt ggtttttatt
    23761 taacattgtc caacatgttt tagcaacaat aaaaataatt acacatgata ttagcacaca
    23821 gactgaaaaa agttttagaa atgcgatgta gtcagtgtta ttgaactcct tcaaaatact
    23881 atttttattc aatgtaataa aaggaatttt tttaattgaa gacaggaaag ataaggtaat
    23941 aaacagaatt atatatatgg ttaaagtcgc agtaagaatg taattgagct ttttcctcat
    24001 agaatcacct cactttctaa cgaaaattat accaaaaagg agagaagaaa taatgcaaga
    24061 attagtaatt ttgaaaaata aagaagctgt aactacgagc ttacaagtgg cagaaagttt
    24121 caaaaagaaa cataagcatg tactagaagc aattgaatca ataaaaagat cggtcgaaaa
    24181 ttcggccaat gttgaagatg ggtccaattt tggacagatg tttgtggaag ggaacgagcc
    24241 agactcatac ggaagaagtc ggagagttta tttcatgaat agagacggat tttccttgct
    24301 agctatggga ttcactggaa gtaaagcaat aaatttcaaa ctaaaattta ttgaagcttt
    24361 caacgaaatg gaagatgtta ttcggaagaa tactgttcct caaacaattg aagacatgat
    24421 gatctatcaa ttagaagaaa tgaaagatgt taaaaaagat gtttccatgc ttaaagatac
    24481 tatgcgaatt agcggacaac aagagtttga aattaagcaa aaaggaaata tgaaagttat
    24541 ggaagttcta gggggaaaag aaagccgagc ttatgaagaa atcagcaaaa aagtattctc
    24601 aaaattttgg tctgaattta aacgtacctt ttcaatccca agatatggcg agttacctcg
    24661 taagagattc gatgatgctg tttcatttat tgaaatgtgg ttaccagaaa ctgcgatccg
    24721 catggaaatc gatcaactga acagacaaca aagacttttc ggtgatgaaa atgaatagag
    24781 ctgaagcgct aagaataggg acggtaattg ctaatcgctg gtggagacac aataaaccaa
    24841 acatcctaag ccaacaacat atcgaaaagc aaaaagcatg gcaacaaata aaaaagtgac
    24901 tcagccggca agcatagagt cacaaaacat atctaaggag aattttagca tatgaataaa
    24961 gaactttcca ctttagatca atatttgact gattctgaat ggggcaagtc gaatatcaag
    25021 gaaacaaata atcgaaaaat cagacgaaat cttttgacga acgaagaact atcatgtgat
    25081 caagatgact tgggaaattt tgtgagtatt tgggatcatg tttaccttat ccatctatca
    25141 aaacattcaa ataaacctga atacatctat gtcatcgaag atggcttgat tgatgcgcta
    25201 gaagagtaca acagagataa cttgattgat atctcttatt acggatcagg taagaaatac
    25261 attgctgaaa tggaggcaga atttgatgag tgaaatcaaa gggacaacga actttgaaaa
    25321 actttttagt cgtaagttaa ataaaattct caagaaaaaa ggaaattttg attatttatc
    25381 ttgggctcac gcgtgggaga ttatgaaaaa gaatgatcca caggcaacgg taactattaa
    25441 tgagtataaa cactacaggg ttgtttctgg aactcatcaa gactttcttg ttgaggaata
    25501 taaacctttt cttatggatg aaacagggac ttatgtatct gtctcagtaa cggttaaagg
    25561 acatacggaa acaga
//
LOCUS       DARBEG010000033        25502 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000033 DARBEG010000000
VERSION     DARBEG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.033"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..3660
                     /locus_tag="SCK20_001362"
     CDS             133..3660
                     /locus_tag="SCK20_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG1145439.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="SCK20_001363"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="SCK20_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG1145440.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="SCK20_001364"
     CDS             7448..7789
                     /locus_tag="SCK20_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1145441.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="SCK20_001365"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="SCK20_001365"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG1145442.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="SCK20_001366"
     CDS             9146..11566
                     /locus_tag="SCK20_001366"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG1145443.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SCK20_001367"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SCK20_001367"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG1145444.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="SCK20_001368"
     CDS             13000..13737
                     /locus_tag="SCK20_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1145445.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="SCK20_001369"
     CDS             13751..14566
                     /locus_tag="SCK20_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1145446.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="SCK20_001370"
     CDS             14579..15226
                     /locus_tag="SCK20_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1145447.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="SCK20_001371"
     CDS             15242..15898
                     /locus_tag="SCK20_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1145448.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="SCK20_001372"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="SCK20_001372"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG1145449.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="SCK20_001373"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="SCK20_001373"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG1145450.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="SCK20_001374"
     CDS             18240..18758
                     /locus_tag="SCK20_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1145451.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="SCK20_001375"
     CDS             18865..19365
                     /locus_tag="SCK20_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1145452.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="SCK20_001376"
     CDS             complement(19645..20565)
                     /locus_tag="SCK20_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG1145453.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="SCK20_001377"
     CDS             20730..21743
                     /locus_tag="SCK20_001377"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG1145454.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SCK20_001378"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SCK20_001378"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG1145455.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="SCK20_001379"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="SCK20_001379"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1145456.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="SCK20_001380"
     CDS             complement(23383..24144)
                     /locus_tag="SCK20_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1145457.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="SCK20_001381"
     CDS             24333..25427
                     /locus_tag="SCK20_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1145458.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8448 a         4424 c         5384 g         7246 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 gg
//
LOCUS       DARBEG010000034        25194 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000034 DARBEG010000000
VERSION     DARBEG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.034"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..746
                     /locus_tag="SCK20_001382"
     CDS             99..746
                     /locus_tag="SCK20_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1145459.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="SCK20_001383"
     CDS             1139..3784
                     /locus_tag="SCK20_001383"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG1145460.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="SCK20_001384"
     CDS             4094..5407
                     /locus_tag="SCK20_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG1145461.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="SCK20_001385"
     CDS             5397..6050
                     /locus_tag="SCK20_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1145462.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="SCK20_001386"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="SCK20_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="SCK20_001387"
     CDS             6787..7872
                     /locus_tag="SCK20_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1145463.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="SCK20_001388"
     CDS             7869..9761
                     /locus_tag="SCK20_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1145464.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="SCK20_001389"
     CDS             9768..10145
                     /locus_tag="SCK20_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1145465.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="SCK20_001390"
     CDS             10299..11081
                     /locus_tag="SCK20_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1145466.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="SCK20_001391"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="SCK20_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1145467.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="SCK20_001392"
     CDS             complement(11996..12055)
                     /locus_tag="SCK20_001392"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1145468.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="SCK20_001393"
     CDS             12447..13109
                     /locus_tag="SCK20_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1145469.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="SCK20_001394"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="SCK20_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13637..13837
                     /locus_tag="SCK20_001395"
     CDS             13637..13837
                     /locus_tag="SCK20_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1145470.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="SCK20_001396"
     CDS             complement(14091..15770)
                     /locus_tag="SCK20_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1145471.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="SCK20_001397"
     CDS             complement(15772..15984)
                     /locus_tag="SCK20_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG1145472.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="SCK20_001398"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="SCK20_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG1145473.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="SCK20_001399"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="SCK20_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG1145474.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="SCK20_001400"
     CDS             19959..20156
                     /locus_tag="SCK20_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145475.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="SCK20_001401"
     CDS             20309..20794
                     /locus_tag="SCK20_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG1145476.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="SCK20_001402"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="SCK20_001402"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG1145477.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="SCK20_001403"
     CDS             21671..22198
                     /locus_tag="SCK20_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145478.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="SCK20_001404"
     CDS             22289..23026
                     /locus_tag="SCK20_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1145479.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="SCK20_001405"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="SCK20_001405"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG1145480.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="SCK20_001406"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="SCK20_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG1145481.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8390 a         4103 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DARBEG010000035        24631 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000035 DARBEG010000000
VERSION     DARBEG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.035"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..81
                     /locus_tag="SCK20_001407"
     tRNA            8..81
                     /locus_tag="SCK20_001407"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="SCK20_001408"
     tRNA            101..173
                     /locus_tag="SCK20_001408"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="SCK20_001409"
     tRNA            201..274
                     /locus_tag="SCK20_001409"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="SCK20_001410"
     tRNA            288..361
                     /locus_tag="SCK20_001410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="SCK20_001411"
     tRNA            385..474
                     /locus_tag="SCK20_001411"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="SCK20_001412"
     tRNA            487..560
                     /locus_tag="SCK20_001412"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="SCK20_001413"
     tRNA            579..654
                     /locus_tag="SCK20_001413"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="SCK20_001414"
     tRNA            656..726
                     /locus_tag="SCK20_001414"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="SCK20_001415"
     tRNA            740..813
                     /locus_tag="SCK20_001415"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="SCK20_001416"
     tRNA            838..925
                     /locus_tag="SCK20_001416"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="SCK20_001417"
     tRNA            947..1018
                     /locus_tag="SCK20_001417"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="SCK20_001418"
     tRNA            1041..1130
                     /locus_tag="SCK20_001418"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="SCK20_001419"
     tRNA            1143..1216
                     /locus_tag="SCK20_001419"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="SCK20_001420"
     tRNA            1235..1307
                     /locus_tag="SCK20_001420"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="SCK20_001421"
     tRNA            1311..1383
                     /locus_tag="SCK20_001421"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="SCK20_001422"
     tRNA            1388..1458
                     /locus_tag="SCK20_001422"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="SCK20_001423"
     tRNA            1471..1544
                     /locus_tag="SCK20_001423"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="SCK20_001424"
     tRNA            1556..1627
                     /locus_tag="SCK20_001424"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /locus_tag="SCK20_001425"
     CDS             1893..3086
                     /locus_tag="SCK20_001425"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG1145482.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3216..4688
                     /locus_tag="SCK20_001426"
     CDS             3216..4688
                     /locus_tag="SCK20_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1145483.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4771..5559
                     /locus_tag="SCK20_001427"
     CDS             4771..5559
                     /locus_tag="SCK20_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG1145484.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5626..6294)
                     /locus_tag="SCK20_001428"
     CDS             complement(5626..6294)
                     /locus_tag="SCK20_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG1145485.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            6409..8121
                     /locus_tag="SCK20_001429"
     CDS             6409..8121
                     /locus_tag="SCK20_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145486.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8124..9893
                     /locus_tag="SCK20_001430"
     CDS             8124..9893
                     /locus_tag="SCK20_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145487.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10098..10664)
                     /locus_tag="SCK20_001431"
     CDS             complement(10098..10664)
                     /locus_tag="SCK20_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1145488.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(10664..11620)
                     /locus_tag="SCK20_001432"
     CDS             complement(10664..11620)
                     /locus_tag="SCK20_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG1145489.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(11620..12276)
                     /locus_tag="SCK20_001433"
     CDS             complement(11620..12276)
                     /locus_tag="SCK20_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG1145490.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            12646..14664
                     /locus_tag="SCK20_001434"
     CDS             12646..14664
                     /locus_tag="SCK20_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1145491.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    14734..14940
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15091..17505
                     /locus_tag="SCK20_001435"
     CDS             15091..17505
                     /locus_tag="SCK20_001435"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG1145492.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            17853..19406
                     /locus_tag="SCK20_001436"
     CDS             17853..19406
                     /locus_tag="SCK20_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1145493.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(19787..21571)
                     /locus_tag="SCK20_001437"
     CDS             complement(19787..21571)
                     /locus_tag="SCK20_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145494.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(21586..23307)
                     /locus_tag="SCK20_001438"
     CDS             complement(21586..23307)
                     /locus_tag="SCK20_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145495.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            23407..24168
                     /locus_tag="SCK20_001439"
     CDS             23407..24168
                     /locus_tag="SCK20_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1145496.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7672 a         4495 c         5196 g         7268 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt atttatctat
     3181 ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca aatgttactt
     3241 tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt acaatcgtaa
     3301 caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg aactgggtat
     3361 tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa cttgcggata
     3421 aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc ggttcgttat
     3481 tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt caagggatgg
     3541 gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat tctattgaaa
     3601 agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt gtttttggac
     3661 cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt tttatcaatg
     3721 tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa ccaaaaatca
     3781 tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc atgcttgtct
     3841 cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt gtttctgctt
     3901 tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa agagctaaag
     3961 atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc aacttggctg
     4021 cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg tggatgcaag
     4081 gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta tcactagtct
     4141 ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact cattgggtct
     4201 tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt atgcctttca
     4261 gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc ttaggtgtga
     4321 ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag cttggtgtag
     4381 caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt tctatttttg
     4441 gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta acaaatgatg
     4501 cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat gcggatattt
     4561 tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta gttggcttgt
     4621 tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt ggtaaaataa
     4681 aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa agttattcaa
     4741 aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa tatttattcg
     4801 aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac aaaaaaggtg
     4861 attggcacca gttcagagca agagacaacg gatatacctg ttttatattt acttcatgga
     4921 atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta tgtagcaaac
     4981 tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga tacagtttat
     5041 gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca tgaattattc
     5101 ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat gggcggatat
     5161 ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat ctctctttca
     5221 ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa agcatctttt
     5281 tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga tccgcaatac
     5341 ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat ggcttgtggg
     5401 gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca ggaacaaggc
     5461 ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg ggaccaatgg
     5521 attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct taaaaagata
     5581 gctcctatag agatcacaaa catagtgatc tctataggag cttttttact gctcgttttc
     5641 tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg ttagaaagag
     5701 tgctttttca ttcaattctg catgggactg catactttct ttaaatccct ctaattgatt
     5761 ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat ttccgtccat
     5821 aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa tcaataaatt
     5881 gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac gaacaagtaa
     5941 ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag tattgatttc
     6001 taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca tatactgcag
     6061 caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt ccattgtttc
     6121 agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag caggtgcttg
     6181 aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt gtgtttcagc
     6241 caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt ccattttatc
     6301 aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt ctaattttac
     6361 agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat gaaattaatc
     6421 ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc cgtagcagtc
     6481 atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga agctattatt
     6541 acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct ggctttgcta
     6601 ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca aggcgtaagt
     6661 gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg aaatatcgaa
     6721 cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca aatccaaaat
     6781 ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat tggtgccttc
     6841 attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct tatcgttacg
     6901 gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat gatcattcaa
     6961 aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat tcgtgtagtg
     7021 aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag tgaggactta
     7081 acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc cttcatgctg
     7141 gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt taaagatgat
     7201 cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat tatgatggca
     7261 atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc gctgaagcgg
     7321 attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc agaacaagag
     7381 ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga ggatacagat
     7441 acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat cgtcggggcg
     7501 actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga tccgacagaa
     7561 ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag tttaagacaa
     7621 acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc tcaaaacttg
     7681 cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc tattgctcaa
     7741 gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga agaacgtagc
     7801 agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt tatcggtaat
     7861 ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc tgaaaagctg
     7921 gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat cgcacaaaaa
     7981 attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag attagtcggc
     8041 gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat ctttgaaact
     8101 caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa tttttctatc
     8161 actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg atcattgcta
     8221 cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa ttagctaatt
     8281 acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct gtaatatgga
     8341 aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt attttgttca
     8401 cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac aaattggaaa
     8461 aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt cgttttacca
     8521 gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc atcacgaatg
     8581 ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag ttagcttggg
     8641 ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc cgcaaagcac
     8701 ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat atggatgaaa
     8761 aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg attgaaggat
     8821 tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct tattctggtc
     8881 tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt atctttttcg
     8941 gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc ttagttgtga
     9001 cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca tctggataca
     9061 gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt gatgaaaaag
     9121 atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt cttgcgttgc
     9181 agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc tcgatcaatg
     9241 tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa acgacgatca
     9301 tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc gatggaatag
     9361 atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt ttgcaagaat
     9421 ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg gacgcttcag
     9481 atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc atgacgttag
     9541 aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca ggtcaaaagc
     9601 agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt ttggatgaag
     9661 caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg gatgaagcaa
     9721 tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg aatgcagata
     9781 gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac gagttattag
     9841 cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa taaagaggtt
     9901 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag cttctcatgt
     9961 ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt gaataaagag
    10021 gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac atatcattta
    10081 tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg cggctggttc
    10141 ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag tttggaacaa
    10201 aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta gacgacaagg
    10261 atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt gcgtgtagtc
    10321 tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt gaaaatccct
    10381 gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc ttgtagaaac
    10441 aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc atcgattgct
    10501 tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc tgctagaaaa
    10561 accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg ttgcaagatt
    10621 tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg ctcacctttt
    10681 tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca tcaattgcat
    10741 tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc cacggcgcat
    10801 cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg tcgcaaatga
    10861 cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag tcccgcaaca
    10921 ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata tctgaatcta
    10981 aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt aatccgttga
    11041 tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca tctaaataag
    11101 tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt gtttgcaatc
    11161 caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca actacttcat
    11221 ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt aaattgacta
    11281 cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta aaattttgga
    11341 aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg atttctttct
    11401 ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa ccagatacac
    11461 tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag tcaaatcctc
    11521 catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg taattccaag
    11581 aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct tctttctgta
    11641 agagttatct atttgttttc ttttttctct atacactgga taagtcaggc acagccaaga
    11701 aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca gataaatgcg
    11761 gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg tgcgtaaggt
    11821 tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa tcatactgcc
    11881 tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg ccaatgaagg
    11941 acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa ttacaataaa
    12001 tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc ataaagccgc
    12061 acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa agagttggta
    12121 gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat cgaaaccaga
    12181 aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga caagaaagaa
    12241 gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt gaagcaatga
    12301 cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac caatgtatga
    12361 ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga aagacatttt
    12421 ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa tccttttaaa
    12481 caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt acggtaaatt
    12541 acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat attaagagaa
    12601 gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga aaacattgca
    12661 agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa acgaggggca
    12721 gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt acagccggtt
    12781 cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac agcggcattt
    12841 atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct atatgcatca
    12901 gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt atctcaagca
    12961 ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt ctacatggat
    13021 acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg acaatatcct
    13081 tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc atttggcaac
    13141 ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga tgcgacagct
    13201 tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa taatatcatc
    13261 gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg cggtggagcg
    13321 actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag caattctggt
    13381 aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg tgattctgtt
    13441 tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg gaacaacatc
    13501 caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca ggaaaatggc
    13561 tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg tacatcaaac
    13621 ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt ttggaagatc
    13681 tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat caagaacaat
    13741 ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa tgctggagca
    13801 aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc aagttcttct
    13861 aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg gagcgtctca
    13921 aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca aaataacttt
    13981 atctacccag gacaacaact gactgtttca aacagtggat caacaaacag cacgtcaggt
    14041 aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg cagcagttcg
    14101 tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc aaataaaaac
    14161 ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt catttatcca
    14221 ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg ttcaactact
    14281 tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc atccactggt
    14341 aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa caaacatcat
    14401 atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat ctatccagga
    14461 cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc agttactggt
    14521 tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga cagtcatgga
    14581 attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat ttatccaggt
    14641 caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt gaattccaca
    14701 aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg aagtagtaaa
    14761 aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc ttttgaactc
    14821 gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg gccgttacaa
    14881 cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta cgtcctataa
    14941 aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag aaaacaaagc
    15001 aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat aatagaaaca
    15061 agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga aattgagaaa
    15121 aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga tcctgaaaag
    15181 ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct acatgtaggg
    15241 catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag tcaaggatac
    15301 aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca atatgcgcta
    15361 gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt ccgacgccaa
    15421 atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac tgatccggag
    15481 tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct agcttatgaa
    15541 gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa tgaagaagtg
    15601 atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat gcgccaatgg
    15661 atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt agttgattgg
    15721 ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg agcaaatatt
    15781 gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg accagatact
    15841 ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag agaaattacg
    15901 acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa aaaatcagat
    15961 ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc ttatgcaatc
    16021 aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt atcttcatac
    16081 ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga attcgctcaa
    16141 actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa agctgcttac
    16201 acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa acaagaagca
    16261 atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga agtcagctat
    16321 cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat cccaatcatc
    16381 cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt acggttgcct
    16441 aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa tatcgaggag
    16501 tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac gaatacaatg
    16561 ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca taacaaaaaa
    16621 gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta cattggtgga
    16681 gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt cttatacgat
    16741 atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg aatgatctta
    16801 ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc tgatgatgtg
    16861 gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg gccattagat
    16921 gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt ggatcgtgta
    16981 tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac attgaatgat
    17041 ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga ctatgaaaat
    17101 cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc ctataaagtg
    17161 gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc gattgctcct
    17221 cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc ttacgcacca
    17281 tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt tttccaagta
    17341 aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga tgagctagaa
    17401 aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac agtcagaaaa
    17461 gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc tctttgtcaa
    17521 taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca ttctgtctca
    17581 gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga ttttcatgtt
    17641 ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct ctgtaatcat
    17701 caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga aattgagagt
    17761 acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga ttttgacgca
    17821 taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt attatcgaag
    17881 tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa agaataatgt
    17941 tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct gtatcgttcc
    18001 tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa ttaaataatt
    18061 tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt gtttttcttc
    18121 tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg cttagtcaaa
    18181 gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat ctttattttg
    18241 agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag ttattgctga
    18301 gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc ggaatgaaca
    18361 cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag tcttcgcctg
    18421 gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc attcttttag
    18481 ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat ataaatgctg
    18541 cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca aatgaagggt
    18601 tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat gatctcaaac
    18661 ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat tgggtatttg
    18721 tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg gggctgattc
    18781 ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca tggtttatct
    18841 attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa acatggcaaa
    18901 cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta ttttatggca
    18961 acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact ttgtacattt
    19021 acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg atcaatcagc
    19081 cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca cgtgaaagtt
    19141 tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg attccggaaa
    19201 actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact caaataagcg
    19261 cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat gcagggaaaa
    19321 tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc gttcagcaac
    19381 tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg ttcagcaccg
    19441 agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt gtaactcgtg
    19501 attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc gtattttaca
    19561 agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt aaaaagggat
    19621 tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc caaaaagtat
    19681 cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa aagctatcga
    19741 tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac gctatctgaa
    19801 attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca gaatgtgttc
    19861 ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt tcaatcgttg
    19921 acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc attgcttgct
    19981 gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa ataacgacag
    20041 gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct tgagataacg
    20101 tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga ataaagtgat
    20161 cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc attttcccgt
    20221 aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa accatcccaa
    20281 aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg aattcgatct
    20341 ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt tttcctgcac
    20401 cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg acttgattca
    20461 tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt tttcctttcg
    20521 gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca tctaatatct
    20581 caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca taagaagcgg
    20641 tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag aagccgatcg
    20701 tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg aaggcgagtt
    20761 gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca cgtttaaaag
    20821 cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct tgctgattga
    20881 atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt aaaacaccca
    20941 gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta gttgtaacaa
    21001 ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg atataaaaca
    21061 gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg atacctgtca
    21121 ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg tcacctactt
    21181 gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt tcttttcgca
    21241 ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata aatgaagtga
    21301 tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat gcttgtttga
    21361 tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag agatcatcga
    21421 tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat aaactgcata
    21481 gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa cgtatcaaag
    21541 cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata ttccttgtga
    21601 ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag taccttgtcc
    21661 aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg tactgaggcg
    21721 ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg ctcttaatgc
    21781 tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga tctctgctgg
    21841 tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat aattagaacc
    21901 gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt cttctaattg
    21961 agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc ccatctgcaa
    22021 gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac tgatgtggga
    22081 gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta cttttcctat
    22141 cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag atccggtccc
    22201 gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt ctagaactgg
    22261 atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat ttgtctgatc
    22321 aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct ctgcttgcag
    22381 tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca ataccatagc
    22441 tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag tcacgacacc
    22501 gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag aagcataaac
    22561 gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt tcactttgat
    22621 catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat atgattgatt
    22681 atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga tggtgatccg
    22741 atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca gtcgataaac
    22801 aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata gagaataacg
    22861 aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca tttggcaaaa
    22921 taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca aaagagatcc
    22981 agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga aatatttctc
    23041 ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag aggcaactgc
    23101 agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt ttataactga
    23161 ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg gtacacctaa
    23221 tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc ctttgttttt
    23281 ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat acttttcgat
    23341 tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa aaagaggtga
    23401 gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga tttgccaaaa
    23461 tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca agaagagaat
    23521 gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt tttttataga
    23581 aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt gacggagtta
    23641 tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt gatgaaacaa
    23701 aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga tacaaacgag
    23761 gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga gtttgaagat
    23821 attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat gagtatgcca
    23881 gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt acatttattt
    23941 actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt cttattgaaa
    24001 tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag aatgtatgaa
    24061 aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac ggatgaaaac
    24121 aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga aatatgtgtt
    24181 taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct ggttcgatta
    24241 cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt tttagaggaa
    24301 atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg taattgtcct
    24361 cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat ggtgaactga
    24421 gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat gagaatgata
    24481 aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac attattacaa
    24541 tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt aatcaaatag
    24601 tccctactgt gactttcctc agctatcggt g
//
LOCUS       DARBEG010000036        24575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000036 DARBEG010000000
VERSION     DARBEG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.036"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SCK20_001440"
     CDS             complement(152..550)
                     /locus_tag="SCK20_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG1145497.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SCK20_001441"
     CDS             complement(547..1320)
                     /locus_tag="SCK20_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1145498.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SCK20_001442"
     CDS             complement(1317..2000)
                     /locus_tag="SCK20_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1145499.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SCK20_001443"
     CDS             complement(2167..3054)
                     /locus_tag="SCK20_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1145500.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SCK20_001444"
     CDS             complement(3051..3920)
                     /locus_tag="SCK20_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1145501.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SCK20_001445"
     CDS             complement(4057..5343)
                     /locus_tag="SCK20_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1145502.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SCK20_001446"
     CDS             5616..6194
                     /locus_tag="SCK20_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1145503.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SCK20_001447"
     CDS             6194..7123
                     /locus_tag="SCK20_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1145504.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SCK20_001448"
     CDS             7120..8856
                     /locus_tag="SCK20_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1145505.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCK20_001449"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCK20_001449"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG1145506.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="SCK20_001450"
     CDS             complement(9752..10261)
                     /locus_tag="SCK20_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG1145507.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCK20_001451"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCK20_001451"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG1145508.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="SCK20_001452"
     CDS             complement(11562..12653)
                     /locus_tag="SCK20_001452"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG1145509.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCK20_001453"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCK20_001453"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG1145510.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCK20_001454"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCK20_001454"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG1145511.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCK20_001455"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCK20_001455"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG1145512.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="SCK20_001456"
     CDS             complement(16616..17590)
                     /locus_tag="SCK20_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG1145513.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="SCK20_001457"
     CDS             complement(17571..19454)
                     /locus_tag="SCK20_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG1145514.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="SCK20_001458"
     CDS             complement(19472..21697)
                     /locus_tag="SCK20_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1145515.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="SCK20_001459"
     CDS             21812..22846
                     /locus_tag="SCK20_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1145516.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="SCK20_001460"
     CDS             complement(22913..23926)
                     /locus_tag="SCK20_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1145517.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="SCK20_001461"
     CDS             complement(24004..24192)
                     /locus_tag="SCK20_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG1145518.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DARBEG010000037        23831 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000037 DARBEG010000000
VERSION     DARBEG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.037"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(55..789)
                     /locus_tag="SCK20_001462"
     CDS             complement(55..789)
                     /locus_tag="SCK20_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1145519.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="SCK20_001463"
     CDS             1116..1526
                     /locus_tag="SCK20_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG1145520.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="SCK20_001464"
     CDS             1519..2220
                     /locus_tag="SCK20_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG1145521.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="SCK20_001465"
     CDS             2220..3806
                     /locus_tag="SCK20_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145522.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="SCK20_001466"
     CDS             3825..4046
                     /locus_tag="SCK20_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG1145523.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="SCK20_001467"
     CDS             4043..4804
                     /locus_tag="SCK20_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG1145524.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="SCK20_001468"
     CDS             5184..5693
                     /locus_tag="SCK20_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1145525.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="SCK20_001469"
     CDS             complement(5768..6307)
                     /locus_tag="SCK20_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG1145526.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCK20_001470"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="SCK20_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG1145527.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="SCK20_001471"
     CDS             7320..8171
                     /locus_tag="SCK20_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG1145528.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="SCK20_001472"
     CDS             8199..8834
                     /locus_tag="SCK20_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG1145529.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="SCK20_001473"
     CDS             8854..9627
                     /locus_tag="SCK20_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1145530.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="SCK20_001474"
     CDS             10035..10832
                     /locus_tag="SCK20_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG1145531.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="SCK20_001475"
     CDS             10810..11223
                     /locus_tag="SCK20_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1145532.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="SCK20_001476"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="SCK20_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1145533.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="SCK20_001477"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="SCK20_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG1145534.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /gene="maf"
                     /locus_tag="SCK20_001478"
     CDS             16000..16560
                     /gene="maf"
                     /locus_tag="SCK20_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG1145535.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCK20_001479"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="SCK20_001479"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG1145536.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="SCK20_001480"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="SCK20_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG1145537.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="SCK20_001481"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="SCK20_001481"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG1145538.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="SCK20_001482"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="SCK20_001482"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG1145539.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="SCK20_001483"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="SCK20_001483"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG1145540.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="SCK20_001484"
     CDS             21907..22485
                     /locus_tag="SCK20_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1145541.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="SCK20_001485"
     CDS             22537..23019
                     /locus_tag="SCK20_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG1145542.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="SCK20_001486"
     CDS             23136..23510
                     /locus_tag="SCK20_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145543.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DARBEG010000038        23065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000038 DARBEG010000000
VERSION     DARBEG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.038"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(111..566)
                     /locus_tag="SCK20_001487"
     CDS             complement(111..566)
                     /locus_tag="SCK20_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145544.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="SCK20_001488"
     CDS             complement(720..1928)
                     /locus_tag="SCK20_001488"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG1145545.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="SCK20_001489"
     CDS             complement(2040..2372)
                     /locus_tag="SCK20_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1145546.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="SCK20_001490"
     CDS             2520..3395
                     /locus_tag="SCK20_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1145547.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="SCK20_001491"
     CDS             complement(3477..4058)
                     /locus_tag="SCK20_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG1145548.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="SCK20_001492"
     CDS             complement(4064..5323)
                     /locus_tag="SCK20_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1145549.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="SCK20_001493"
     CDS             complement(5325..6371)
                     /locus_tag="SCK20_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145550.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="SCK20_001494"
     CDS             complement(6606..6881)
                     /locus_tag="SCK20_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG1145551.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCK20_001495"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCK20_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG1145552.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCK20_001496"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCK20_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG1145553.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="SCK20_001497"
     CDS             complement(9980..10660)
                     /locus_tag="SCK20_001497"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG1145554.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="SCK20_001498"
     CDS             complement(10709..11344)
                     /locus_tag="SCK20_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1145555.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="SCK20_001499"
     CDS             complement(11410..12813)
                     /locus_tag="SCK20_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG1145556.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="SCK20_001500"
     CDS             complement(12800..13831)
                     /locus_tag="SCK20_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145557.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="SCK20_001501"
     CDS             13862..14083
                     /locus_tag="SCK20_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG1145558.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="SCK20_001502"
     CDS             complement(14248..14985)
                     /locus_tag="SCK20_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG1145559.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="SCK20_001503"
     CDS             complement(15138..16478)
                     /locus_tag="SCK20_001503"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG1145560.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="SCK20_001504"
     CDS             complement(16715..18238)
                     /locus_tag="SCK20_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG1145561.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="SCK20_001505"
     CDS             complement(18616..19227)
                     /locus_tag="SCK20_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1145562.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="SCK20_001506"
     CDS             complement(19557..20273)
                     /locus_tag="SCK20_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1145563.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCK20_001507"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCK20_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG1145564.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="SCK20_001508"
     CDS             complement(20832..21629)
                     /locus_tag="SCK20_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG1145565.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="SCK20_001509"
     CDS             complement(21599..21970)
                     /locus_tag="SCK20_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1145566.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCK20_001510"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCK20_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG1145567.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaa
//
LOCUS       DARBEG010000039        22936 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000039 DARBEG010000000
VERSION     DARBEG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.039"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            58..612
                     /locus_tag="SCK20_001511"
     CDS             58..612
                     /locus_tag="SCK20_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1145568.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(785..1258)
                     /locus_tag="SCK20_001512"
     CDS             complement(785..1258)
                     /locus_tag="SCK20_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1145569.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1270..1806
                     /locus_tag="SCK20_001513"
     CDS             1270..1806
                     /locus_tag="SCK20_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1145570.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1846..2217
                     /locus_tag="SCK20_001514"
     CDS             1846..2217
                     /locus_tag="SCK20_001514"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1145571.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2501..3772
                     /locus_tag="SCK20_001515"
     CDS             2501..3772
                     /locus_tag="SCK20_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1145572.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4017..5042
                     /locus_tag="SCK20_001516"
     CDS             4017..5042
                     /locus_tag="SCK20_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1145573.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5336..6445
                     /locus_tag="SCK20_001517"
     CDS             5336..6445
                     /locus_tag="SCK20_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1145574.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6528..7424
                     /locus_tag="SCK20_001518"
                     /pseudo
     CDS             6528..7424
                     /locus_tag="SCK20_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7440..8264
                     /locus_tag="SCK20_001519"
     CDS             7440..8264
                     /locus_tag="SCK20_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1145575.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8296..9606
                     /locus_tag="SCK20_001520"
     CDS             8296..9606
                     /locus_tag="SCK20_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG1145576.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9754..11013
                     /locus_tag="SCK20_001521"
     CDS             9754..11013
                     /locus_tag="SCK20_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1145577.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11023..11895
                     /locus_tag="SCK20_001522"
     CDS             11023..11895
                     /locus_tag="SCK20_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1145578.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11907..12737
                     /locus_tag="SCK20_001523"
     CDS             11907..12737
                     /locus_tag="SCK20_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1145579.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12777..14960
                     /locus_tag="SCK20_001524"
     CDS             12777..14960
                     /locus_tag="SCK20_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1145580.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15021..16241
                     /locus_tag="SCK20_001525"
     CDS             15021..16241
                     /locus_tag="SCK20_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG1145581.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16238..17698
                     /gene="gtfA"
                     /locus_tag="SCK20_001526"
     CDS             16238..17698
                     /gene="gtfA"
                     /locus_tag="SCK20_001526"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG1145582.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17775..18881
                     /locus_tag="SCK20_001527"
                     /pseudo
     CDS             17775..18881
                     /locus_tag="SCK20_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19203..19412
                     /locus_tag="SCK20_001528"
     CDS             19203..19412
                     /locus_tag="SCK20_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1145583.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19405..19554
                     /locus_tag="SCK20_001529"
     CDS             19405..19554
                     /locus_tag="SCK20_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG1145584.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19708..20373
                     /locus_tag="SCK20_001530"
     CDS             19708..20373
                     /locus_tag="SCK20_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1145585.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20657..21742)
                     /gene="xerS"
                     /locus_tag="SCK20_001531"
     CDS             complement(20657..21742)
                     /gene="xerS"
                     /locus_tag="SCK20_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG1145586.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22076..22309
                     /locus_tag="SCK20_001532"
     CDS             22076..22309
                     /locus_tag="SCK20_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG1145587.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22306..22713
                     /locus_tag="SCK20_001533"
     CDS             22306..22713
                     /locus_tag="SCK20_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1145588.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINPIKTHTQPI"
BASE COUNT         7836 a         3440 c         4389 g         7271 t
ORIGIN      
        1 atggtaaaat atatacataa tcattaactc agaatatttt taagaaggga aacaatagtg
       61 agctataatg tacaaccgtt aaggacaaat gaagagatac aagattttca gttttggcta
      121 agaagaacaa gtaatccgga aagagatagt tttttatttt tgtttggaat caataatggg
      181 ttacgtatgt cagatattat aggattaaaa gttggagata tacgaggcaa aagtaagcct
      241 attattgttg aacataaaac aggtaagcga aaaccgattt ttatagataa tttgagagaa
      301 gaaattttat tatatactga aggtaaagaa gaaaatgatt ggttatttcc gagtcggcaa
      361 caaggtcgac atataacaag ggatcgtgtc tatcagatat atgcagatat agcagaaaaa
      421 ttaggacgag atgatatagg aacacataca ttaagaaaaa cttttgggta tcactactac
      481 aagaaaacta gagacattgc taccttaatg tttattttta atcatagctc gcaagcaatt
      541 acaaaaaggt acattggtat aactgaagat gaaataggag catcacttcg tggatttaaa
      601 ctaggtgtat aatttaaaaa tttttataaa cagtaaagac aaagattaaa agctattttt
      661 atctagaacg attttatttc ataaagttcg cggaataaaa gctggggtta ctggtacttg
      721 gtataggttc gtgttatata aactaagtaa ttaaataaca tttttaggtt ggaaaaggat
      781 gaaattaaaa aaagagaatc aatacggtct caaaaacgca aacgctgcgg aataagaaaa
      841 atactgataa ctcaacgttt agcagactat ctaataggga tagtctgctt ttttcgtttt
      901 tgaccatact tttgaccttc tgcaaacttc cttgcttttc ctctgtagaa ctcatgaaat
      961 ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc gatatgggtg tatatatcat
     1021 ccatagtggt tttaatatct gtatgtccaa gtcgaacata cacttctttg acagacgcac
     1081 cagattcaaa gagcagacta caatgggtat gacgaaaacc atgaagtgtg atatgtttta
     1141 agttattctc tttaatgatg attttcaaat tgtgatttac atagtctaaa taaaggggct
     1201 tattttcttc tgatgaaaac aaaagttgtt caccatcttt tataggtttg attctcattt
     1261 ttagatatta tgcccgcacg tattcatcac atttctattg tgaatcgctt tattcgaccg
     1321 acatttgact tttatcataa tatactcggt ttaaaattac taatgaaaac aattaatcaa
     1381 gacgatcata ccatgtatca cctgtttttt tcagataatc accaccgtgt gggaactgaa
     1441 ctgacttttt ttgaagtaca agaagggaac aaccagtttt ttggaaccaa tacgattgaa
     1501 cgaacgatac taaaagttcc ttccgaagca tcattgcatt tttgggaaat atattttgaa
     1561 acacaaggcg tttgccatta tggaatcgaa tcattttctg gacgacctat cttacgtttt
     1621 gaagggccag atgcaacacg attagcttta gttcccttac gcgaatttga agatattgat
     1681 gattatttcc cgtatgtcac agaccagatc cctacagagc atgccatttt aggaattgat
     1741 gccattcaac tacgcgtaca atattccgat gcgactgaac gagagttatt gtcggtaggc
     1801 tggtagcaca aagaatacgt cagtttcttt tcgggtcatc aacgagtgac aatcttagaa
     1861 aatcaagaca ctcagttcta tcaagaagtt cagattattc aagatcgtga gaatccagtt
     1921 gccattgaag ggattggtag cgtccatcat gttgcgtttg gcgtagaaaa taaatctgat
     1981 ttacaacgca tcgacaaaca attacaagag cgcaatttta taaattcagg aataaaagat
     2041 cgtgaatttt ttgtatcact ttattatcgt gacccaaatc aattattgat tgaaattgct
     2101 acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc aaagtccacg ctttgaagaa
     2161 attcctttat ttttaccgca gtacttaaat ttcattcgtg aacaagtgga gcaataatta
     2221 gctaatcaaa tccatgacga agagtaagag gttgactcat aagtcaaaaa ataaagaaaa
     2281 acgcatcaaa tccattctaa agaatgttga tttgatgcgg ttcctatttt tataaactat
     2341 cattttcgac taataaaact acttatgagt cagcctctct ttttttcttg atttttatac
     2401 ctctttctag gtaaactaaa catctgccaa aaataccgtg tctcattgaa acattctcaa
     2461 ctatttcaaa ataaagggga gtaccaaatg aacgagtata ttgaaattat ccacgcaaga
     2521 gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat aaaattagta tttttaccag
     2581 cgtatctggt tcaggaaaat catcgattgt ttttgatact attacccaag aagctggtcg
     2641 tcaactgaat gacacctatg atagttttac ccgtttattt ttacctaaat acagtcgtcc
     2701 agaggttgat gagattgata atctttctac ggcgattgtg gtcaatcaaa agaagattgg
     2761 tggcagtgcg cgctcaacgt tagggacgat tactaacatc aatgcgttat ttcgtgtgtt
     2821 gttttcatgt tttgcaacac ctagtttagg atatgcaaat gcttattcat ttaatgatcc
     2881 tagtggaatg tgttctgtct gtcagggaat cggtaagacg attgcatttt taataggatt
     2941 gctttatttt tcggctattg ataatgctga ttttgtattt tctgatccat ttaccgcaga
     3001 agttttgtct ggaattgagg aacaggcaaa gaatcatggt tattttgtat tggtgcataa
     3061 tgttaattca gcagaagatg ttaaaactat tcagagaaat tggcgttttg aggggttcat
     3121 tgttgttggt gtacgtgtaa aagaattttt ggaattaaat aacgtttttg atggtcctgt
     3181 tgtttatatt gatacgcatc tttcaaaaga gacattaaga gaacttgaac aacaaatgaa
     3241 tcgtttattt gtaaatacaa atgattacca agctagtaca ttggcagtgg aacatttgat
     3301 tcattatggt catgagaaaa tagcgtttct ttcttttgac tacattgtag gtgaaccaag
     3361 tgttattcaa gaacgacatc aagcttatat cgatacatta aagaaatatc atttaacctt
     3421 taaatcgcat tggatatata acaactctga atttgagcga atttatcaag aattagctga
     3481 atttactgca attgttgtta ctggagatta tttagcggct aaattagtaa aatttttaaa
     3541 aaataagaat aaacgagtga tggaacaatt atcgattatt ggatttgatg atattacttt
     3601 tgcagaatta atggatcctg cgttgacaac aatacgacta aatccaggta acaagggggc
     3661 gattgctttt caccaactga ttggactggc caaacaagaa gaaataaaaa atcaagtact
     3721 actattagag ggagaattga tttctcgaga ctctgttcac aaaatcaaat aaaaaagctc
     3781 tgtgaaataa gtatagttaa caataaaata tccgaacgat agagagaatg aactgtgatc
     3841 tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat ttactctaaa ttgttcggat
     3901 attttttctt gtttttaatt atgattacag tgcctatttt taacgaaatt tgcttatttt
     3961 caaaatgtat gattatatgt tagtaaacac tttcaaatat gaaaagagga gctattatgg
     4021 taggaattag ggatgttgca aaacgggcgg gtgtgtccat tgcaacagta tcgcgcgtgc
     4081 taaatgagga tccgacactt tcagtaaccg atgaaaccaa acgaaagatt gcagaatcgg
     4141 ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa aaatttaaca aatattgcat
     4201 taataacgac agtttctgaa gtagatgagt tggaagatcc ctattttcga acgattcgca
     4261 gagggattca agtcgaagca gaacaaagca aagtcaattt gaagaaaatt attcgtttgt
     4321 cagaaacacc gttggagcaa gacgatttgc taaaatgtca aggtgtgttg attattgggc
     4381 aggtgctatc ttctgtgatt gaagaagtat cagcaattaa ttcgaatgtc gtagtgattg
     4441 atgaccctag tattgaagct gaagtagatg cagtttatac tgatttaaca aaagcaacga
     4501 atcagcattt agatcgattg tataaaaaag gacaccgaaa cattgcgttt atcggaggag
     4561 gacgtgttcg tttagatagc caaggaaata aacacgtgtc agatgatgat tatcgaaaaa
     4621 tagcctatga gacgtggatg aaacaaaaag gattagaaaa atatagtcaa atttatttag
     4681 aaggctggac cacacttgac ggaatgcagg cagcagattt acttttgaaa aaacatgcta
     4741 aagataagtt gccgactgct atcatggtag gaaatgatcc tattgctgtg ggggtttatc
     4801 gttcacttca aaaaaatggt ctacatattc cagaagatat ttcgattgtg agttttgata
     4861 atattgaagt tgctgaattt ttaacacctg cattaaatac ggtgaatgtg aatacagaag
     4921 aaattggaaa actagcagtt cgcatggtta atgaacgaat aaaagaagtt agaaatgttt
     4981 cgatcaaagt gattgtttct aataatatta ttgttagaga aagtgaaaaa aatatcgatt
     5041 aaaaaagtaa acatccaaaa aagggtgttt acttttttgt ttactaatat ttttagtaaa
     5101 aaatctcgac aaaacattaa gtaaacagtt atggtgttgt taacaaaaag aaaacgcatt
     5161 caaaacagga ggaattaaga tgaaacattt caaaaaaata gctttaacag ggatagctgt
     5221 tttaactggg acattactcg cagcatgtgg tgggggagat agtaattcta aagacgggca
     5281 agtaacttta agctatgcga gttgagatac tgaccaagct ccaggactaa gaaaaatgtt
     5341 agatgaattt gaaaaacaaa atcctgatat caaagtagaa atggagacaa cgccttggga
     5401 tcaatattgg gttaaattag aagctgcaac aacgggaggt aatatgccag atgtagtaac
     5461 catgcactct agcgaatcct ataaatatat gtcacagaat gctttgatgg gacttgatga
     5521 tgttatcaaa gaaaatgata ttgacatggg aaactttact gaaggaattg ctgatttcta
     5581 tacgcttgaa gacgaactat acgcaatccc taaagatgct tctgtagtgg ggctatggta
     5641 caacaaagaa ttatttgata atgcaggtgt cgattatcca gatgaaacat ggacatggga
     5701 aacactcaga caagctgcaa ttgatttaac agatgaatca aaaggcatct atggttttgc
     5761 atcacatatt ggaacagaga ctggctactg gccatttatt tatcaaaatg ggggagaagt
     5821 atttgccgat gacaatacaa aatcaggttt gaatacccca gaagcacaag aagctttagc
     5881 tttttatcga gatattgcac ttgttgatca tgcttcacca acaattacag aattacaaga
     5941 tcctgataag ttaagtcgtt ttcaatcagg tagattagca atgatgattg aaggaaactg
     6001 gtttgcaccg ggatttttga aaaatgacta tctagttgaa aatgttgctt ctgccgtgtt
     6061 acctaaggga aaagttaatg caacaattac aaatggactt ggttgggctg ccagtgcgag
     6121 tacaaaatac ccagaagaag tgaaaaaact aatggcattt ttagctagtg aggacgccaa
     6181 taaaatccaa gcggaaacgg gggcatcaat cccagcacta aaaggctttg aagacgagtg
     6241 ggctgcttct tcagatacaa ttgatttgag tgcttttgga aaaatgttgg agtatggagt
     6301 ccctcgtcca tacaataaag atggattacg agctgaacaa gcagaagttc aaattattaa
     6361 ccgtattctg tctggtgaag taactgttga agatggaacc gctcaaattg aaaaagaagt
     6421 caatgcgata ttgagcggaa attaaattag ttaattatac aatatacagg cgaaaggatt
     6481 ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg ggatattatg aaaaatcgaa
     6541 tttttggaaa atatcgtaaa gatcaagtga tatggggcta tatttttatt tttccaacgg
     6601 cattaggatt aattttatta aatatatggc cagcaattca aacagcaatt ttaagttttc
     6661 aaaaaacggt agggttcggt gatactgaat gggtcggatt aagaaattac caaaaaatgt
     6721 ttgcagacgg tgaagttttt cagtctttat taaacacatt aatttatgca gtggtttcga
     6781 tacctagtat tgttgtatta tccttgttgg tggcagtttt aatgaataga aaagttaaag
     6841 gattgtcgat ttatcggaca atttattttc taccagtcgt tgctgcacca tccgcagtcg
     6901 caatgatttg gcgctggatg ttcaataatg attatggact gattaataac atgttggcta
     6961 aaataggtct cgatggacca gcatggttaa cggatccatc gattgcgatt tattctatta
     7021 tcattgtagg agtttggagt gctattggtt ataacatggt gttattacta gccggcttat
     7081 aagaaattcc taaagattat tatgaagcag cggaaattga tggtgctagt ccaatccagc
     7141 agtttttcaa cattacgtta ccattagtta caccaacatt attttttgta gttgtaacaa
     7201 cagttatcaa tgctttccaa gtatttgatg ttatttttat gatgattact cctaatagta
     7261 ccgccatgtt taaatcacaa tcattagcct atttattcta taaacattca tttattttaa
     7321 atgacaaggg atatggttcg gcaatcgtta tgttcctttt agtgattata ttaattatta
     7381 ctgcaattca aatgaagttg cagaaaaagt gggtaaacta ttaaggaggc tatcaaatta
     7441 tgaaaaataa atttttttca aatgcgccaa tccatatcat gcttatttta ggtggtattg
     7501 caatggcatt accttttatt tggatgattt taacttcagg gaaaacgttg gttgaatcta
     7561 cccaaattcc acctacaatt ttccctgaga aatttcagct aagtaactat caacaagtac
     7621 ttgatttatt accattcatg gctttttata tcaatacatt tttaatggtt gttggacgag
     7681 tgattggctc ggttttattt tcagcaatgg ctgcatatgc ttgtgcgcgt ctaagttttc
     7741 cagggaaaaa tatccttttc agcttagtac ttattcaaat gatgattccc ggttccattt
     7801 ttattactcc acagtattta ttagcacaaa aattgggtat tttaaataca attccagcat
     7861 taatattacc aggggtagtt agtgcgtttg gtacgttttt attaagacaa tttttcttag
     7921 gtttacctga tgaattggag gaagcggcct atttagaagg tgccaatatt tggacgatct
     7981 ttttcaaaat tatgttgccg cttgctaaat caggattggt tgcacttgga atattcacag
     8041 cgttgtttgc atttaaagat ttaatgtggc cattgattgt caatacttca attgataaga
     8101 tgcctttagc tccgggatta gcaaacttac aaggacaata tgccacgaac ttcccacaat
     8161 taatggcagg ttcgttatta gcaatttggc caatagttgt gatattcatc gttttccaaa
     8221 gaaaattcat tgaaggaatt gctacaacag gtagtaaatt ataaaactat taattagtga
     8281 ttggagagta agtacatgac aaaaattaca tttattggtg caggaagtac tatttttgca
     8341 aaaaacgtat taggcgatgc gatgttaact ccaagtctac aagatgcaca tattgcattg
     8401 tacgatattg atgaaaaacg actaaaagaa tcaaatttaa tgcttaaaaa tattaataaa
     8461 aattcgaatg aaaatcgtgc tgatattgtt tcttattatg atcgcaaaga agcgttaaga
     8521 gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt ataaaccttc aacagtgatt
     8581 gattttgata ttcctaaaaa atatgggttg cgacaaacta ttggtgacac catggggatt
     8641 ggtgggattt ttagaaccct acggacacta ccagtaatgt tagactttgc taaagaaatg
     8701 gaagaagttt gtccaaatgc ttggttattg aactatacaa atccgatggc gatgttaacc
     8761 atgggtgttt taaaagcgac gagtattaag actgttggtt tatgtcatag cgttcaagta
     8821 tgcgtaccag aattgtttga gcatttagga atagccgatc aatatgactt aaatgacttt
     8881 caatggaaaa ttgcaggcat taatcatatg gcattcttgt tggaaattac aaaaaatggg
     8941 gaagatttct atccaacaat tcgtcagtta gctgcagaaa aaccaaatcc acatccagat
     9001 tctgtacgtt ttgaattgtt gaaacgtttt ggttactata ttacagaatc aagtgaacac
     9061 aatgcagaat accatccata ctttattaaa tcaaaatacc cagaattgat tgacgaatta
     9121 gggattccgt tggatgaata cttacgtcgt tgtgaacaac aaattaatga ttgggaaagc
     9181 atgcgagagg acattgtaaa taatggggag ttaacacata cgagaagccg tgaatatgct
     9241 tcatatatca tggattctat tacgacagga actccgtcag tgattgcagg aaatgtctta
     9301 aataaaggct taatcacaaa cttaccgaca gatgtttgtg tggaagtacc ttgtttagtc
     9361 gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc caacacaatt agctgcttta
     9421 aatcggacga atatcaatgt tcaagaactg acagtagaag ccgctttaac actagataaa
     9481 tcaaaaattt atcaagcagc gtatttagat ccacatctat catctgaatt gtctttaaca
     9541 gatatcaaaa atttagtgga tgatttaatc gaagcacatg gggactattt acctgaatat
     9601 aattaaaaag aagctgaaag agtaaggaat tttcaattcc ttactctttt cttagttaaa
     9661 tttagtaaaa atattgacaa aacacttagt aaacagttac attagtgtta acaaaaagaa
     9721 aacgcattca aattgtataa ggaggagcat aaaatgaagt ggtctaaaaa aattatttta
     9781 gcttgtatag cactaggagc aacgatctct ttgactgctt gtggaaaaaa tcaggaagat
     9841 gcaagcggta aggtagaaat agagtttttt aatcaaaaaa aagaaatgac tcaaacgatt
     9901 caggagattg ctaaagattt tgaagcaaaa aaccctgata ttcatgtaaa agttgtggat
     9961 gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg caggcgatgt accagatgtc
    10021 attaatcttt atcctcaaag tatagaattg aaagaatggg cgaaagctgg ttatttagag
    10081 gacttaaccg aagaaccgta tttagagaat atcaaaaatg gatatgcaca acgttttgct
    10141 attgaggatc gtgtatatag cataccatta accgctaatg tctatggttt ttactataac
    10201 aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa cttgggcaga atttgaaaaa
    10261 atagtagctg agattaaaga tcaaaacaaa gtaccgtttg cgattgcagg agcagaaggc
    10321 tggactttaa atggttatca ccaactagct atagcgactg ctgctggtgg tgaagaggaa
    10381 gcgaacaatg tttggcgttt ctcagaagtt aatggaatta atgctgaaag caaagaaatg
    10441 caaaaagatt ttaatcgatt agatttgtta agagaaccaa aagctttgca aaacaactgg
    10501 caaggtgccg gttataacga tacagtagtt acttttacaa aaggtgatgc gttgattatg
    10561 ccgaatggtt catgggcaat gccgatgatt cattcacaaa atccagattt tgaagtagga
    10621 acttttcctt ttccagcaga tgaagcaggt caaagtctaa cgattggcgc aggagaccta
    10681 gccttatcag tttctgcaac ttctcaacat aaagaagaag ccaaacgttt tgtagaatat
    10741 atgacaacac ctgaagcgat gcagaaatat tatgatgtag atggtgcacc gtgtgctgtt
    10801 gagggcgtta tcgagaatac tcaggattca ccattaagtg gtttaactga acttgctttt
    10861 actgatcgtc atttagtttg gttagccaaa gattggaata gtgaaaatga tttttatacc
    10921 ttaacaacaa attatttaca caatggcaat caaaaagcca tgattaacgc attaaatgct
    10981 ttctttaatc caatgaaagc tgacggaagg taggaaaata aaatgaaaat aaaagaaatt
    11041 ttaaatcgtt actgggcaat catttttgta gcaattccaa ttattttgca gatgattttt
    11101 ttctactttc caatggttca aggtgctttt tacagtttaa ctaattggac tggtctgact
    11161 tataattttg attttgtggg tttgaacaat tataagattc ttttttctga tccgaagttt
    11221 atcaaatcga ttggcttcac tttaattgtg acgctttgca tgattgctgg acaaattggt
    11281 tttggtattt tgattgcgcg ggcgttgaac gcaaatatta aagggaaaac attctttaga
    11341 gcatggttct tttttccagc ggtcttatcg ggcgtggttg tttctttagt atttaaacaa
    11401 atttttaact atggtttacc aaaaattggt agtatgttag gcattgaatt tttacaaacc
    11461 agtcttttag gaacaacatg gggagcgatt tttgcagtaa tttttgtttt actttggcaa
    11521 ggaattgcca tgccagtcat cattttctta gcgggttttc aaagtattcc acaagaaatt
    11581 attgaagctg ctagaatgga tggggcgaat gataagcaaa ttttttggaa tattgaaatc
    11641 acctacttat taccaagtat ttcaatggta tttattatgg cattaaaagg tggcttaaca
    11701 gcatttgatc aaatttttgc tttaactggt ggtggaccaa gcgatgccac aacatctttg
    11761 gggttacttg tttataatta tgcttttaaa aacaatcagt ttggttatgc gaatgctatt
    11821 gctttagtct tatttatctt aattgggatt gtttcactgg cacaattaaa attatcgaaa
    11881 aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa agaaaaaaat atttctaaat
    11941 acttgctttt attattaggg ggcttcatga ttttagttcc cttagtttca acgctattcg
    12001 gttcatttaa aacaacaccc gatattatga ataatttctt tcgttttcct aatccagcga
    12061 ctttggaaaa tttccgaaga ttgttagcgg atggaattgg tcattacttt tggaattcgg
    12121 caatcatcac aatcattgca gtagttgctg tgacagcaat tattcctatg gctgcttatt
    12181 cactagcaag aaaaatgact cgtgggaaag catacggatt gatgtattta tttttaattt
    12241 taggaatttt tgttcctttc caagtaatta tgattccgat taccgttatg atgagtaaaa
    12301 ttggtttagc aaatatgtgg ggattaatct tactctatct cacttatgcc gtaccacaga
    12361 cactattttt atatgttggt tttattaaac taaatgtgcc tatcagtctg gatgaggctg
    12421 ctgaaattga tggggctagt cactttacaa cttatttcaa aattgttttc ccaatgttaa
    12481 aaccaatgca tgcgacgact attattctta atgctctatg gttttggaat gatttcatgc
    12541 taccattatt aattttaaat cgtgattcta atatgtggac actaccattg tttcaatata
    12601 attatacggg acaatatttt aacgactatg gtccaagttt tgcttcctat acagtaggaa
    12661 ttgtcacgat tacactagtt tatcttgctt tccaaagaag tattatagct ggaatgagca
    12721 atggagcagt gaaataacca ataaattatt ctcattataa aggagttcat aaacttatga
    12781 ttatatttga caatcacaca caaacatttc atttacaaaa taaaaaaatt agctatctat
    12841 taggaatcga agaaaataat tatttaacgc atctttattt tggaaaaaag attacgcact
    12901 attctggtgg gtatcgctac ccccgaacag atcgttcctt ctcacccaac aatgcaaatg
    12961 cgcaagatcg tttattatct ttagacacat taccattaga atttccagga tatggacatg
    13021 gtgattttag agaaccggcg cataatatta aattaggaaa cggtagtcgt gtaaatgatt
    13081 ttcgttatga tagctacgaa attcaaaaag gaaagcccag cttagcggga ttgccagcct
    13141 tatatacgga agatgatgat gaagctgaaa cgctgattat tcaattgaaa gacgcgatta
    13201 gtggattgac tttacagttg ttctacacaa tttatcaaaa tcacagtgta attattcgtt
    13261 ctacgcaatt agtaaataat agtgatgaaa cagtggagat taaccgctta gctagtcaat
    13321 caattgattt tccaaatcgt ccactcgagt taattcattt aaatggtacc tgggcaagag
    13381 aacgtcaaat gactagagaa aaaattcata cagggataaa agtattagat agtaaacgcg
    13441 gatcaagcag ccatcaacaa aatccgtttg ttgctttagt agatgccaaa acagatgaat
    13501 ttcaaggaga ggcatatgga tttaatttag tttatagcgg taatcacgaa acggtgattc
    13561 aaaaagatcc atttgaccaa acacgggtca ttatgggaat taattcattt aattttggtt
    13621 ggcaactaaa aactggcgac tcatttcaaa cgccagaagt cgtgatggtt tatagtaatg
    13681 aaggtctgaa tacgttatca cagacgtatc atgaattgta taatcaccgt ttaattcgtg
    13741 gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa ctgggaagca acgtattttg
    13801 attttaacga agaaaaaatc atgaatattg tcgatgatgc gaagaacttg ggcattgaaa
    13861 tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa tgacttcact tcattaggtg
    13921 attgggttga aacagaaggc aaacttgctt gtggtttgga aaatttagcc aagcaagtgc
    13981 atgataacgg gatgaaattt gggatttggt ttgaaccgga aatgatttct gaagatagtg
    14041 aactatttcg agcacatcca gattgggcat tgggtattcc aggtcgtgac cgctcaatta
    14101 gtcgaaatca atatgttctt gatttttcaa gacaagatgt tcgtgataat atctatcaac
    14161 aaatgacggc gatttttgat cgtgtaccga ttgattatgt gaagtgggat atgaatcgaa
    14221 atatgacaga agtctattct gtcttgttgg actcaacgat gcaaggtgaa gtggctcatc
    14281 gttatatgtt agggttatat gaatttttag aaaagttaac aacgaattat ccaactattt
    14341 tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc aggtttcttg tattacatgc
    14401 cacaaacttg gacgagcgac aataccgatg ccgtggctcg tctgaaaatt caatatggaa
    14461 ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt ttcagcagtc ccaaatcatc
    14521 aaacaggaag agaaacgagt ttaactattc gtggcaatgc tgcgatggct ggtgtttttg
    14581 gttatgagct agatctaacg caattaactt ctgaagaaaa agcagaaatt aaagaacaag
    14641 ttgcctttta taaaaagcat cgccaacttt tgcaatatgg aactttttat cgcttggaaa
    14701 gtccgtttga aggcaatgat actgcatgga tgtttgtctc aaaagaccaa acagaagcca
    14761 tggtctttta tttccgagta ttagcagaag ctgcgcatcc attagtcaca ttgaaattag
    14821 caggattagc agaagaagca acttatcaaa tggaagatgt aacatttgct ggcgatgaat
    14881 tgatgaatct cggtttttat attgactcag aactacatgg tgattatgcg acacaacgct
    14941 tttatttgaa acagatatag tagcttgaat ttacaatata agtgcaacac caaaagagac
    15001 tcattccaaa cgaagaaaat atgttttatt tacacgcatt tactaaaaag cagcctgatt
    15061 tgaattggga aaatgaagaa gtacgtgaag aaatctatgc aatgattaac tattggttag
    15121 ataaaggact aggcggattt agaatcgatg cgatattaaa catcaaaaaa aaactagagt
    15181 atggcatctt tgaatctgat ggagaagatg ggttagcctt cattgggccc tggattctta
    15241 atcagtcggg cattgaagtg tggttaaaag aaatgagaga gcggacattt aaaccacata
    15301 atagtatgac ggtagcagaa gccgatgttc ctaatgaacg tttagatgaa tatattggtg
    15361 aagatggcta ttattcgatg gtttttgatt ttagttatac ggatattgat gtacctgaaa
    15421 ctggtgaatg gtttaaagat agccaatgga catggacgga tatgcgtacc aatattttta
    15481 cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt atatttagaa aaccacgatc
    15541 aaccacgttc aattaataaa tatattccag aagaatggat caatgattat agtaaaaaaa
    15601 tgttggcgac gctttttatg ttactaagag gtaccccttt tatttatcaa ggacaagaac
    15661 taggaatgac caacatagaa atggcatcat tagaagattt tgatgatgta gctacgcatt
    15721 ctcagtataa acgggcatta gaatatggat tggcaccaga gcaagcattg aaagctgtca
    15781 ataaacgaag tcgggataat tctcgcacac ctatgcaatg gaatacgcaa aaaaatgcca
    15841 gattctcaac ttctgataat gtatggttaa aagtaaatcc taattatcca gaactcaatg
    15901 cagaagctca gttaaccaat caacagtcgg tattcaatta ttatcatcaa ttaattaaat
    15961 taagaaaaaa tagtgtatac aaagacgtgc taatctacgg acaatttcta ccagtgaaag
    16021 ataaaaatga acatattttg ctttatgaac gccaattaga agataaaatc gtattagtta
    16081 tcttaaacgt tacagcaaca gaacaagaat attatgtaga tgaacagtac caacaagtat
    16141 taatcaataa ttatgaagat gttttattag atcaaagtcg aaaattgcgt ttacgaccgt
    16201 ttgaaagtat tgtattagct aattatggag gacaattatg aaaattaaaa acgaagcaat
    16261 gctcatcacc tatgcagata gtttaggaaa aaacatgggc gaattaaaac aagtcttaga
    16321 caaacattta caaggtgtta ttggtggtgt acatctgttg cctttttttc catcgacagg
    16381 tgatcgaggc tttgcgccga gtgattatac gacagttgat ccagctcttg gaacatggga
    16441 agatgtggaa gcattaggtg aagattatta tttgatgttt gattttatga ttaatcacat
    16501 ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac catgagcagt caccatacaa
    16561 agaaatgttt atccgtattc atgaattttt cccagaaaat cgaccaacac aagaagatat
    16621 tgatttaatc tacaagcgta aagacaaagc gccgtttcaa acggtagaat ttgcggatgg
    16681 tacgacagaa gaggtttgga atacctttgg ggaagaacaa attgatttag atgtgaccaa
    16741 agaagttgtg aaagaattta ttcgagaaac catcaaagat atggcgaacc atggctgttc
    16801 tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa ttagatacca atgatttctt
    16861 cgtggaacca gatatttggg aacttttaga cgaagtgcgt caagaagcag cgaaatatga
    16921 cgtcgaatta ttaccagaaa tccatgagca ttattccatt caaatgaaaa ttgccaatca
    16981 tgattattat atctatgact tcgccttgcc aatggtcact ctgtattcac tttatagtgg
    17041 aaaatcagaa cgtttagcca attggttgaa aatgagtccg atgaagcagt ttacaacatt
    17101 ggatacgcat gatggaattg gtgttgtgga cgctcgtgat ttattaacag atgaagaatt
    17161 ggattacacc tctgaggaat tgtataaagt cggggcgaat gtgaagaagg tctattcaag
    17221 tgccaattac aataatttag atatttatca aattaacagc acctattata gtgcattagg
    17281 cgataatgac cgtagctatc tgttagcacg tgcgattcaa gtatttgcgc cagggattcc
    17341 acaaatttat tacgttgggt tattagcagg aaaaaatgat attgaactgt tggaagcaac
    17401 gaaagaaggg cgtaatatta atcgtcatta ctatgattta gccgaaattg aagaagaagt
    17461 gcaacggcca gtgattcaac aattatttgc acttttaaaa tttagaaatg aatctgcggc
    17521 gtttgatttg gatggaacga ttgaagtagc aacgccatca gaatcagaaa tagtgattac
    17581 acgtaaaaat aaaaacggta gtgatagtgc aacattaacg gctaacttgg atacgaaaga
    17641 atttatcatt gagtctaaag atataaaaat tttcactagt gttgcattaa aattttaacc
    17701 tattccatag aacaaaaaat cctttatact agcacttgga tgacttatcc aagaccaata
    17761 aaaaggactc atacatggat aagtataaaa caaatacagc atttaataaa tggttttctg
    17821 ccataaaatt aggaaaactt tctgtcaact cccaaaaatg tatttttgat tacaattcgt
    17881 atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt tctctacgca gttaatgaag
    17941 aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga ccgatcgctc caactagaaa
    18001 ccgatgtgtc atcgattagt catacccaat tgtctcgagc atttaatgca cttgaaccta
    18061 aagtgttaga agaaattttt caacaactgt tagaaaaagt tcaacatcaa atcaaaccaa
    18121 ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt ttcgcttagt ttaaaacgtc
    18181 ataagtgggc gactttccgt caaacaaaat caggtgtcaa gttacactta aactattgtt
    18241 atatggacga tactacgatg tatccaactg attttacctt aacaaatgcc catgaacatg
    18301 atctcaatca actaccagta ttggtgaatc aaccagaggc aacttacgtg tttgatcgtg
    18361 ggtattagga ctttgaaaag atggatcaga tgcattggga tggctatttc ttcgtcactc
    18421 ggattaaaaa aaacaccaaa gttcacgtga tagatactct ggaaacatcg ccagaaacag
    18481 aaattctacg agacgagctg gttcgtttag gatctaaaac ctatctaacc gccaatttcc
    18541 gactagtcac tgttcaagat aagaacggac gtgtgtttca atttatcacc aatcgaatgg
    18601 acgtctcttc aaaagaaatc tctgatatgt atcacgctcg ttggcaaatc gaattgtttt
    18661 tcaagcacat caaacaacac atgaccatta aaaccttctt ttctcaaagt gaaaaaggcg
    18721 tgcagaatca attgatttta acgatgattt ccgctctcct cacgtttctc atcaaattgg
    18781 agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt ctttcgttat ttactgtttc
    18841 agccctttga atgttgtcta gaaaaattaa ttcccacatg agaaaaaaga aatttggctc
    18901 gttacttttg gttttcagtt gaataacaga taaaaaattt tctggaaaaa gtcatcctct
    18961 gcgtcagcac agtttttttt gacttatttt cagaggaaat agttttcaga atattctaaa
    19021 atgttttatg caacactagt gatatttttt agaattagat ttctaatgtg cattttttga
    19081 gtattaagga gcaagtatac aaagatttat aaagctgatt aaatttgaat aaaaatatgc
    19141 aggaacgcta ctatttttag tagtgttctt ttttatattt gaataaaaaa tgtggtggtt
    19201 aaatggtggt tataacaaaa aatgaattaa tgaaattagg ttatgggaag tatcagtctg
    19261 agagaatcat acgacaggca aaagcaatta tggtttcaaa aagctatacc tattacgaaa
    19321 atagaagatt aggactagta cctgttgaat ctgtagaaga agttttaggt atgaagatag
    19381 aggtagagga ggattttcag aatcatggtt aattttaaaa aagataaaaa tgggaaatat
    19441 cattttactg ctagtttggg attcgacaaa cgaacaggga aaagagtaca aaaaaacgta
    19501 gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc gaacttgttt ctaattatgg
    19561 aaaagaagca gaaacttctc aaagtactat gttgtttgag gagttttttt ataaattatt
    19621 cctaccttat tatcaaggga aagtagaaga aagaacatat aaaaatcggt tatctacaat
    19681 caaaaaaact tttcttattt ctttaaaatg gagttaagag aaatacgtcc aattgacatt
    19741 caaaagtggc aaaataattt attgagcaaa tataaaaatt cttatgtaag aaacatgtat
    19801 gggatttttc agatgtcatt agatcgagca gttgttttgg gactacttga aaaaaatcct
    19861 gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag ttgatttttg gacaaaagaa
    19921 gaatttacaa aagttatttc aactttttat attgatgatt tgtaccaaca ttttggtttt
    19981 atttgtatgt ggttactttt tatgaccgtt atgcgtttgg gagaagccac tgcattaact
    20041 tggaaagata ttgattttga aaataaaaac ttgcgtgtga ataaaacact ctattataag
    20101 agagccaaaa cctatgagtt tgtagaaccc gaaacggctt ctagtattcg tacaatcgaa
    20161 ctagacgaca atacaattac ctttttaaaa gcttggaagg agcgtcaagt ttcaatagga
    20221 ggcatgtcgt ttgtactttc ttataatgga acacctactc agaaatatac aatccgtcat
    20281 attatgagaa gacatgcaac gatcgttggt gttcatataa tccgcataca cggattaagg
    20341 catagtcatg cttctttgct tatatcacaa taaaaagatg cacttcgaat taaagaaagg
    20401 ttaaaacatg ctgatgtaca aacgacttta ggaacttatg gtcatttgta tctaaattca
    20461 ttgagagaga taacaaatga tcccacagat tatatccaaa ctaatttttc taaaatcaaa
    20521 ccgacaccta tttacgataa taaaaaataa atgataaaca aaagtaggat cacaccaaaa
    20581 gaaacacact agaaaaacta taatacaggc ttttctagcg tgttgtttct tatttccgcc
    20641 tatttataca ttatactcat aaagcattta atgcatcgat cgtagccgca ttgtctatat
    20701 gggtatataa agttgttgta ttggtacttg aatgtcccaa ttgttgggca gttaatacca
    20761 atgaatttgt ctgttggtag agtctagtag ctaatgtatg acgcagtttg tgagggctga
    20821 cttgaattct aaagcctttt gaatattttg ctaccatccg ttcgattgca ataccggata
    20881 tacgcttggc ttctccttgg taaattgcta gaaataaagc tttttcgttt tctgttactt
    20941 tatatcgttc tttccgaata gctagataat tagctaaata ttccattgcg aatgctgcta
    21001 tattgacagc atctctttta tttccttttc tagttactac cacattattc ttatctaaag
    21061 ataggtcatc taaattaata tttataacct cggagagtct caatccagat cctaaaatca
    21121 gtgcattgat cgccaggtct ctttctacat ctcgttgata atagatcaaa gctcgtttac
    21181 tcaaagtatt tttatattca ttggcaatat aatctagata attttgtgta tccttatcta
    21241 gaaataattt tttctcaatg gcattggcac gtgcagctaa cgtagtttta tctgtgatgg
    21301 ttgctacttg atccatagga ttattgtcta agtatggttt tcctagagga ttattggaac
    21361 ttttcgaaag atatttaaat agaaccttta atgccgtgat atttctattg atagtagaag
    21421 tagatagacc taagttatta ttatctttga gcttagtttt tgttgtctct ttttttacat
    21481 tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt taggtcatag gcagttaact
    21541 gtcttatcga ggtgatagaa gtatcaggaa cgatagactc actcagaagc cattcataaa
    21601 agagcttata attttttaga tattctaaag tggttcgagg tgattgatct ttatcctcac
    21661 gatcttgaac aaaatctttt atataatcgg gcatatgaag tatgattgta tctatttttt
    21721 gatatatttc tgtatttttc atgtcattca tcctttcaat aagtagggga gaagagtatc
    21781 tctataattg gactatacca attatagaat gttttgtcaa ttggtatgct ctaaaattta
    21841 ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt taataattta aaataaatgg
    21901 tatacataaa tagggaagga aatagaaggg agcaatctaa attttttgaa ttttcttaat
    21961 taattatata gaaagaacaa tctttctata gagttagaca tgagagggac tatgcttgat
    22021 ttaaaatcaa caagcgtata tactgtatat acaaagaaaa aggagggaga agactatggc
    22081 aattaaagaa agaaaattta gggaagtggg gaactcagta gtattaacta tttcaaagga
    22141 gtttcttaaa gaaaatgggt taaatcccgg agatacggtt tttttagatg aagaaaaact
    22201 tcgagcggca attacaaaaa aacaagcctt atctgatgaa attgatttag caatatctca
    22261 agcattaact gaatatgata ctacttttag agagcttgtc gataaatgag tattcattac
    22321 ctagaagcca aagatattat aaaaatgaat gtaatccaaa taaaaaaata ctctcctaat
    22381 gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt gtgtagggca acttcgagcc
    22441 tcagcttttg gtgaagaggt ttatccttcg atttatgaaa aagcagctat cctacttata
    22501 caattgataa agaaacatcc ttttcacaat gcaaataaac gtactgcttt cttagctact
    22561 tttgtctttt tgaaaatcaa tggacagctt ttagctattg atcaaaaaga tgctgtaaat
    22621 ttagcggtat atattgctac gtatgataag gatttcgatc aactaaaata tgaagtgatc
    22681 aaccctataa agacacatac gcagcccatt taaggagtta ctgtatgtgt ctttataggg
    22741 ttattagctg ctatagcgaa ataattgaag taaattacaa atagctataa ttcaaatcca
    22801 actatataga aaacgtaaat tttctatata gttggattaa gataacgact atttatttta
    22861 tcatattttt actataccct atacttttac ttggtatatt tatgaataat cgcaaattta
    22921 cccctatact actagt
//
LOCUS       DARBEG010000040        22149 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000040 DARBEG010000000
VERSION     DARBEG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.040"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..113
                     /locus_tag="SCK20_001534"
     CDS             <1..113
                     /locus_tag="SCK20_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1145589.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(184..1494)
                     /locus_tag="SCK20_001535"
     CDS             complement(184..1494)
                     /locus_tag="SCK20_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1145590.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1506..2924)
                     /locus_tag="SCK20_001536"
     CDS             complement(1506..2924)
                     /locus_tag="SCK20_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1145591.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3164..4732)
                     /locus_tag="SCK20_001537"
     CDS             complement(3164..4732)
                     /locus_tag="SCK20_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1145592.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4736..5017)
                     /locus_tag="SCK20_001538"
     CDS             complement(4736..5017)
                     /locus_tag="SCK20_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1145593.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(5014..5937)
                     /locus_tag="SCK20_001539"
     CDS             complement(5014..5937)
                     /locus_tag="SCK20_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG1145594.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5934..6812)
                     /locus_tag="SCK20_001540"
     CDS             complement(5934..6812)
                     /locus_tag="SCK20_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG1145595.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6799..8136)
                     /locus_tag="SCK20_001541"
     CDS             complement(6799..8136)
                     /locus_tag="SCK20_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1145596.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8139..9242)
                     /locus_tag="SCK20_001542"
     CDS             complement(8139..9242)
                     /locus_tag="SCK20_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1145597.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9316..10824)
                     /locus_tag="SCK20_001543"
     CDS             complement(9316..10824)
                     /locus_tag="SCK20_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1145598.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10790..11482)
                     /locus_tag="SCK20_001544"
     CDS             complement(10790..11482)
                     /locus_tag="SCK20_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1145599.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11504..12478)
                     /locus_tag="SCK20_001545"
     CDS             complement(11504..12478)
                     /locus_tag="SCK20_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1145600.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12778..13650
                     /locus_tag="SCK20_001546"
     CDS             12778..13650
                     /locus_tag="SCK20_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1145601.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14232..16430)
                     /locus_tag="SCK20_001547"
     CDS             complement(14232..16430)
                     /locus_tag="SCK20_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145602.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16662..17654
                     /locus_tag="SCK20_001548"
     CDS             16662..17654
                     /locus_tag="SCK20_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1145603.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17714..19135
                     /locus_tag="SCK20_001549"
     CDS             17714..19135
                     /locus_tag="SCK20_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG1145604.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19128..20627
                     /locus_tag="SCK20_001550"
     CDS             19128..20627
                     /locus_tag="SCK20_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG1145605.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20666..22060
                     /gene="uxaC"
                     /locus_tag="SCK20_001551"
     CDS             20666..22060
                     /gene="uxaC"
                     /locus_tag="SCK20_001551"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1145606.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6717 a         4516 c         3838 g         7078 t
ORIGIN      
        1 ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa
       61 tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca
      121 tatatcatta aaaaaaataa aggtcaagct aaaatattat ttaagcttga ccttattata
      181 atttcaacga ttgtcaaaag tattgtttgc agctgtgttg ccagtttcta tagataaatc
      241 acagcttttg aaacataagt tttgtactgc ttcgattcgc ccgaattctt ttgcagttat
      301 ttggcaatga gaaaagcaga catcttctat aggcttttca ggaaatgcta ctaaatcaaa
      361 agctcgggaa ggaagtgagt aacctggaga aaggcatgcc tgtacgtttt tgatgtgtat
      421 gtctttgact ttcgtcattt gctttgattc aggtatttct tcagccactg cttgccaact
      481 ttctggcatt tgagcaaggt cattgctatt ttttctattg tattcattat gccagtccat
      541 tatccaagaa aaaggaaatt gtacatcaat catattcaga ttttctaccc tgatattttc
      601 gatcactccc cctctttctt ttgaagattt cattcgaata ccacagtctg actgaaaaaa
      661 attgatatcg tgaatatata cgtcactaac tccagcacta acttcgctac caatagttac
      721 accgtatcct gaacggattt tacatctagc aacctcgatt cttgatgatt ttcgattgac
      781 tcttgcacca ttgccatctc tgccagactt aatagcaata caatcatcac cacaggccaa
      841 atcacattca tagacacgca catttgttga agaatcaata tcaatcccat ctgtacttgg
      901 tccatcattg ttgcggatga ttactttttc tactaataca tcgttagaat aagtgatttg
      961 caagttccaa aaacctgatt tctgaaaaat caaatcacga acaacaacat gttctgcatg
     1021 atacagtaaa caagcgcgtg gacgtttaat agcataatcc gcaatccaac gaagtccctt
     1081 ttgatcgtat tcagctcttg tcccgctttt ttgatctttt ccccaataca gctcccacca
     1141 atggtctcct tgaccgtcga ttattccttt tccttctata agaatatctt tcgccgagag
     1201 aacattcaaa atcgctgctg gccatttcat ttctacgccg gctactcgtg tatcgatttc
     1261 tggaaatgct tcgatatctg ttctcccctt gaggactgca ccttcttcaa agataagatg
     1321 actcttatct tttaaaaaaa gggctccgac taaataaatg ccttttggaa taaatacagg
     1381 taagtgttct ttcgcacctt gatcgattgc tttttgcagt attattgtat catcttgttg
     1441 agtgtcgttt gttttaaagt tcaaagcatt gataatccct ttttttgtcc gcaatacgtt
     1501 tctccctatc tttcattgat ttttttcaat tcttcaccaa taaatacagt ttttgaacga
     1561 ttcagtgggt aacctactag caaaatgatt gcggatacaa ataagcagaa tgctggtaac
     1621 aagtttgcca tcgtataaat cctttcttgt actaaaactg attggatggc tccacctgtt
     1681 gtagacgatt ggtaaccaat gagtgccagc ataaatccgc ctaccgctcc tgcacatgct
     1741 tgtcctactt ttctcgcaaa agaatttact ccatatactg ttccatcttc tcttaagcct
     1801 gtcttatatt gatgataatc aatcacgtca gtaatgaatg cccagaccat caaattgaat
     1861 aaaccagatc ctaaagtagc gataaataaa agaatcagat atacccatgc atttgttata
     1921 tgaaggaaat acatcagcaa gtacatacat gaagaagcaa gtaacgcaaa tacgcttgct
     1981 tcctttttac caaataattt tgtcagcttt gtagcaaatg gtgctaaaac tactactgtt
     2041 ccgtaagtaa ataaaagagc aacagacatt gcttgtttat tatggaagta atcattgaat
     2101 aaataagtca tattcgtccc agctaaaatc tgattgatca caatgaaaat gtcaacaatg
     2161 actaaaataa tcagtgctcg attagctaac aaacctttta ccaactgaga agcagatact
     2221 ttttcgtttt tgacaactct tactctttca gtagtcaatt tacaagttaa catatatgcg
     2281 acaaatgcta ttgcagcaca tccgattgaa atccaaaaga aatgattccc tgaaacgact
     2341 tgttgtccac tcttgttatg ggtatacatt aaaatcggaa tgaagaatcc tgtagttgat
     2401 ccgccaatcg cagaaccaat gcttcggaac gtggacaacg aggtgcggtc attaggattt
     2461 gagctaattg ctgctgccat tgctccatac gggatattga ttgttgagag aaaaatccca
     2521 taaacaatgt atgaaacaaa aatataagca atcttcaccg tcattggaag atgttggacg
     2581 aatggcaaaa acaaaatgac agtcactaag caaaatggat acttcatttt tctgatccaa
     2641 ggaataaatc ggccagtttc tgttaactct gagacatcac ataatcttcc aaccatcaca
     2701 tcagcaaacg catccaaaat tctcgaaatc aagaataacg tacctacgat tgcccctgaa
     2761 acgccaagaa cattcgtata ataaatcatc aagaaactat tgactaaacc taaaataaaa
     2821 cagttaccta gatcaccgaa catgtagcct atcttatcgg aaatcccaaa ttcctttttt
     2881 tcttttttta ctgtatttgc tgtaacagca ttttgtgaaa gcatttgaat tcctcctacc
     2941 tcttctagta aacgttttca aaattataac tctttttttc ttgtttttat attcacaatg
     3001 ttggcttttt attgcattat cttgacagat aaaaaacaaa aaataatcta aaaacattga
     3061 tgcataagat tgataacttt ttttacataa caatatatac aaacaaaaaa attcactaac
     3121 tagtgaaaat aagttagtga acctcattta ttaaagaatt aaattacaga tgatcttttc
     3181 catcatactt gaaaaactca aaatcagcat aacttcctct ttttttattt agatcatgca
     3241 ctgctatccc gataaaattt ccagtaaacc ctcctgagag tatacacatg tttttttgcg
     3301 gacctacttt cttccattct tcagttgata cgtctttcaa atgaaactgt gctgtctctt
     3361 tctttacagt gactttcaaa aaagtcttct tttctgtgag tggaatcgtc caatcttcta
     3421 tttgtgctac tccgttttct gattctatca tacgtaggca ttttcccttg tcctcattcc
     3481 aagtaacata acaataaata taattggatt catttaagta cagtaccagt ccagccatct
     3541 gattaaaatt attaggcatg tattcaaatg ctgtctccgc atcaaaagta aaatccgttt
     3601 gccgaatagc taacaaatga tgttcgaagg tagattgaag agattcaccg gaaatcagac
     3661 gtaaaaaacc tggtctacat tttgtatcac accaatcagc atttggcaaa atgcgtctgg
     3721 cattccagtt cttctttagc tctccttcaa atgtatccat aaactgatga tctttttctt
     3781 gttgtacctc ttcatttgtc tcaatcatta tttcttccat cggagttgtc tggctatctt
     3841 taagtcttaa ccaatcgtcg gacgtccaat acacttcttg aatagcggtt tctcttccca
     3901 aaatagccgc accgtttaat ggtctggtac aaagatatgt catgtaccat cttcctgacg
     3961 gggtttgtac taagctccca tgtcctgtac attgcaatgt agaagcaggt ttgtcactag
     4021 ctgtcagcat aggaaaacca ggatcaagct cgtaaggacc agtaatattc tttgctcggc
     4081 aaactgtaac tgaatgtcct cttcctgtac cgccttctgc cgtcatcaaa taataatacc
     4141 cgttacgaaa atacaaatgt ggtgcctccg ttttcgctaa ttctgtacct gcaaatattt
     4201 tatagatggg accatcaagc ttcttcttct ctgcatcata ttcttgtaat acgataccgc
     4261 tggatttatt cggtgtgtcc agacgataat cccatagttc attgacaagc catttttttc
     4321 ccgtttcaag atcatggaac aaagaaggat caaagccgtt actatttaaa taaattggtt
     4381 cggaccaagg acctgtgatc tcagatgcag tgatcaaata attgtggaca tctttaaacg
     4441 gacggcctgc acttttgaca tctgtgtaaa caagataaaa caatccatca gcataactga
     4501 tctgtggcgc ccatatactt ccatttgtag gatttccttg caaagatact tgagtggtca
     4561 aaatatccgt tgtatgtgac cagttgacta aatttttcga ctcatatact cggattcctg
     4621 gaagccattc aaaagaggag acaataatgt aataccgctc tttcactctt aatatattag
     4681 gatctggatt gaatcctctt aaaataggat ttttcacttt gatagtctgc atgattcagg
     4741 ctccttttct cattcttact cgatataaac tgaatgcccc agctgctaaa aaagcaagtg
     4801 aaataaacaa cacaacggca aacagaccag tcatgtcctg taataatcca aatgtatttt
     4861 ctaaagacaa agttgaatga tctacagcaa gcgactgata acttttttta aactctgacg
     4921 cagacatatt acttaaagta actgctaacc cgccaaagaa aatcaaagat agactgccac
     4981 cgaccattcc tagcaatagt tcccattttc ttttcatttc tgaaccccct gcttttctaa
     5041 atgtatccat tcaggtcttt tgatcgatgt ttcataattg ttattacgtt cagagaattt
     5101 gacagtctgt tcatttttct tgtcatccca attatcccaa ccagttgggt gaacatgccc
     5161 acctatctta cacgcttcaa ataagacatt tgcatacgga cgccacggtc ttcctaaata
     5221 gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt tcaaaacgat accctgtttt
     5281 ttgatttttc ggcgtagatg ctgctgtgat gtatccagga tttttactgg ctaagctatg
     5341 gatatgacac tgatgaaaaa cggcatctgc cccaccaaat atgaaatcca ccgttccttc
     5401 aatccagcaa tattcatagg tctgacgaca atactcagga ttggcagaag ccggtgtaca
     5461 aaaaggagta ccatcttttt gcaaatcagg caacggaccc gtgcataagg tatcctgata
     5521 accagatagt cgacagtttt ttaaagatac gcgatctcct gaattgaata aagcaacagc
     5581 ctgtcctacc ttttctcctg gacctgctgt attttcgatg tgaagatttt ctaatacgac
     5641 attcgatcct tctaagaaaa aagtagcact tcggaaagtc cctaactggc taccgtcaag
     5701 atactcctgt tttgcaaaac gattaccgga gataactact gttcctgctc ccacaagttt
     5761 aaaattggat aatctgcttt ctatatattc atcatatctt ccttctttta ctatcaagac
     5821 ctttggatgg ctatccggca ggcctgccaa ataatcgatt ccttcttgga tagtttcaaa
     5881 atccgtcggg gtatctatag cattctttcc aattatcagt ttgtctttca tcctcataaa
     5941 tgctccctta tgaattgttt ccaaatattt ctcatttctt gtgtttccat atgaccgcct
     6001 gttaaagaaa aagaagaaaa gttttctgga ctacccattg attgatactt tttgtccagc
     6061 ccttttctca gcttcagtac gccttcgtcc aagcacatcg tatcgttttt tccaactaag
     6121 ctaagacgag ggcgcggagc gattttttct tggattgcta acgttgtata agctgtcaat
     6181 agatttggca catagtaata aaacccatga tgatctaggc ggcgatgtag caaaagtgtt
     6241 tcgatgtcca cctgtgccgc tagatcaata caaactttga cacgagaatc caatgctgtc
     6301 acccaccagc ttaataagcc tcccatagaa agaccaatcg ttgcgagccg ttgagtatct
     6361 gtgtcttccc ttgtctccaa ataatcgatc aagctgatca catcataaat acgcattccc
     6421 cataacgttt ttcctgttaa taaaaattgt ttgaacaact cgctttcaga aatacctccg
     6481 cgttcttcaa atccccaagc atcaatcgac caaacagcat atcccattcc tgtcagtact
     6541 tctgcaaaag atggagaagc taagtaagga gcaccatgga tcaattcact tttcccgact
     6601 gaaaaatctc cgccgtgaga atgcaggtaa aggaccgtcg gaaacggtcc ttctttttct
     6661 ggataagcat agagggctgg tactgtttcc aattcattta agtacaattg atagtattca
     6721 agcataaaac cgttttgttt ttctcggtat aatcgttttc cactaggggt atgtctgcta
     6781 gatggagcac ctaaaaaatc attcaactgc ataagcaaag tttttacgaa caaccagttc
     6841 atccatattt tctgattgat ttgattggaa tttctcaata ctgatttctt tagtattttc
     6901 taatagaaat gctggtccct cgtgattttc aatcgacatc tggttaaagg aaaccttacc
     6961 cgcaaagcca atataaaatc cttgtctgct catttcctct attcctgtca tcatagcagg
     7021 aacaccggga actgcatctt gggccatcga gacatcgata ttgtcaaatg tgatttcttg
     7081 tatgaattgt tccgctaatc cataaataaa acctgcagaa gcatggacat tccttgccgt
     7141 gatattcgaa aaatggatcc ttctgaatgc tgatgtccgt tcatctatcg gataagcctt
     7201 tttttcccat acatacggtt ctttccctcg cggaccacag aaataataaa gattcagtat
     7261 aaatggacac atcacattat ccatgactat attggatact cgaatatctt caacgatccc
     7321 gcctcgacct cgtctagatt tcaatcgtat tcctcgatca gtttcttgga agatacaatt
     7381 tgaaatggta atattacgaa tactaccact catttcactt cctaatacga caccgccatg
     7441 tccatggacc atcgtgcaat ttgtgatcgt gatattttcg caagcgatcc tttcgtacgt
     7501 atcttcagtc cctgccttga ttgcaataca gtcatctccc acgtcaatat ggcaattact
     7561 aatacgcaca tttttgcatg attctggatc aattccgtct gtatttggtg aatccgctgg
     7621 gttcaagatc gtcaagttat caaacgttgc attactgcaa agaatcgggt tgatcgtcca
     7681 acttggtgac tggatcaatt taatatcttt cacagtaatt cgatgacagt tatggaaact
     7741 catcaattta ggacgaggat aagcgagatt atctggttca tttctaaaag tatgccacca
     7801 tttttttcca tttccatcta atgtaccgaa accagtgaca gagatgttct ctacattttg
     7861 agcatagata caggaagcat aaacttttcg atggactcct tcccaacgtg aatgaacaac
     7921 aggataatct tttggatcgt cagagaattt cagaaccgct cctgcgctta aatgaagttc
     7981 gacattgctt tttaaaaata aagctcctgt tagaaattca cctgctggaa caactacggt
     8041 cccgcctccg tcacttgctg cagcatcaat ggcttgttgt attgcttcag tattcaattc
     8101 atcaattgat gcgccaaatg tcaaaatatc atacatattt ataatgcctc cacttcaatc
     8161 aatgcttgca agaatgcacc aacgcctttt tgatcattac aaataattgg ttcactgatg
     8221 tagtaagcat atgaaccatc tcgctgatct gctccgccta accctgctac ttgacagttt
     8281 ttattcaagt tcagccagcc ttcttttgtt tccagaacaa attcatcaag taatccttga
     8341 tgtgatttct tcacttgtgg aagccattct tctttgttca gcagtcccaa acgtattcct
     8401 ttagccattg cgtaaacaaa catactactg ccggaagctt ccagataatt tcctttccgt
     8461 tttgtttctg ttgttacttg ataccatgtc ccgctgtttt cttcttgata tggaagtaat
     8521 gcagccagtg tcttttctag aatttctttc agcaaagaac aatctgtttc ttcatgaagt
     8581 aattcatagg tatctgctgc agccatcaaa taccagccaa tcgatcggct ccaaaaatga
     8641 tgggatagac ctgtttttgg atcagcccat ggttggacct tcttttcatc ccatgcatga
     8701 atcagcaaat ttgtttttgg atcgaataga tgtttataac tgatttcaaa ctgtctcaaa
     8761 acatcttggt aatctttttc ttcactaaaa ttctggataa attcagcata aaaaggagaa
     8821 cccatatata agccatccaa ccaaatttga taaggataaa tttctttatg ccaaaaagct
     8881 ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa ggagttctgc tgctttttta
     8941 tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa gctttcctgt atttacatgg
     9001 tcgatattgt attcttcaac gctgtagcca cggatcgttc catcttcttg cacgaaatag
     9061 tcgatattgt cttttatgaa atcaaaataa attttttctt gggttcgttc ccaaagtaat
     9121 tggaaccctt taagtacgac gccatagtca taagaccatt ttcctttata ccacttttct
     9181 tcatataagc gaggtgttct ttccatgata ctttttgcta atttctttga ccagttttcc
     9241 attatttgct ctcctttaaa aattaaaata ggatttggga caaataccaa gaagtatttg
     9301 tcccaatcgt ctatattact gtgccgctgc tttgtatgct tcctcgtact cttgtgtgat
     9361 tcgatcgcct ccttgtgctt tccagtcttc tacagctttt ttgaatccat cttcgtcaag
     9421 ctgtcccatg atgtattgca ccgtagcatc aatgacgatt ttttcaattt ctgatcctac
     9481 ttcattgttc gtcggtgaat ccaatggcac agaaggatcc attacagcaa attcttcatt
     9541 ttctttgatc aattgattag agaattcttt ttcagggtct gtatctttga aagagaacgt
     9601 cacttcacta ggacgagagc ttgataacgg ttgcacctct tgttgccaaa gatccatatc
     9661 gataaattcg atagaaccat catcattcaa ttcataatgc gttccttcga taccgccagt
     9721 catcaattta tacacatctt cttctaaaag atcatcaaca aattgaagta atttcttcaa
     9781 ttgtgcttcg tcttttactt gagatttggg aaatgctagc aagccgccaa taccatttcc
     9841 ttctgaccaa acatgataac cgccgccttc accatctgag atcttgttta caggcactaa
     9901 ttccatatca tcttgaatac cttgacccat cgtcttcaga ttcttgatat ccaccattcc
     9961 agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc tgatcagtct tttgggtaac
    10021 tgcaaagtct tgtgccaaat agccattctc aaatagatct cttgaaaaat tcaaagcttt
    10081 catatactct tctgtatcaa attctggagt gaatttgcca ttatcgtcta cagcccagcc
    10141 atttggtgct ccaaaataag aagtgaaaag tttaaagctg gtataacgaa tatcactagg
    10201 cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt ccatcaggat cgttttctgt
    10261 aaattgttta gctacttcat aaagctcatc catggtagtt ggaacatcta gtcctagatt
    10321 atccaaccag tcttttcgaa tgactaagcc agcacgagcg tagtcttttt gtatcggtac
    10381 gccgtacaag gcaccgtcga tactcgcagc ttcgattcta tcttcagaaa tttgacttaa
    10441 atttttatag tcttttaaat attctgatac atcccagaat acgcctgatt ttaacgcatt
    10501 gcggacagaa gagtttttca acatcgcaat ttgaagtgta acgatatctc ctagttcatc
    10561 agaagccaat gcagttgtta atctttcttc ttttgaagca tcaggaatcc agttgaatgt
    10621 cagttccgta tcggtttttt cttcgatcaa gttcacaatc gtgtctgtag gcggtgaagc
    10681 atgatgcaaa atatttaacc aagtgatttt agcttttcca tcttctgtct ctgaagaact
    10741 gttacttgaa ctgctgccgc atgcggcaag tgctagaatt cctgcaaaac taagacctaa
    10801 ttttacgagc gttgcttttt tcataaattt tctccttcac tgttttaata atatttttgt
    10861 tttcttctgt tttttctgta tatgagttct tccattccaa atggcaaaat atagaatttg
    10921 ctgtccacgt gactgcggaa aagaagagac tgccaccgat aaaggttaaa acacctggca
    10981 ttgtttggat gatcatcaga taaatcccta ccatacttaa tatcaacact aatgagtaat
    11041 gaagatgtga gaatccgaat aaaaatgcca ttttcatttt gaaaaaatat cctttccaat
    11101 cataatgagc catgattggg aaaatataga gtgctgagag cacatatatg aacgcagcaa
    11161 tatacaaagc gatcctaaga taccaattgt gtatgaaaga aagatccaca agtaaaacaa
    11221 tcccgatggc gccatatatc cagctcatca gcaaggtttc acgatagttt tctttaaaat
    11281 atgtgaagta cgttttcgtc aaaggaaacg tttcttttga gcgcagccat tttctaatca
    11341 ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac tcccaaacct gcgatgacta
    11401 aaccgcccca cagaaaattc aaataaacca actgtaaaat acgatttagc cttccgttta
    11461 ttttatctaa tgactgcagc atatctttcc ctctctttct atcttagtaa acgccgtctt
    11521 ctcctagctt tttggcaatc ttattagcta aaattaccat aaacaatcct acgacccctt
    11581 taaacaatcc cattgctgta ctgaaactta actgtccatt cttcaatcca gctgtataaa
    11641 tataggtgtc gaaaatttct gcaacgcttc gattcaatga attcaataac aagtacatat
    11701 gctcgaaccc taagtctaat gtctgaccga tttttagaat cagcaaggta atgattactg
    11761 gacgaattgc cggcaatgta acatgccatg ttttgcgtaa gcgtcctgct ccatccattt
    11821 ctgcagcttc atacaactgt gtatcgactg cagttatcgc tgaaaggtag atgatcgtag
    11881 accagcccaa ctctttccag atcacttgtc cgatatatac tgtgcgtgtc cattcagggg
    11941 atgttagaaa actgattttt tcaaatccca ttgttgcaag gaattcattc aatacccctc
    12001 cgtcaacatt caaaaataca taagtaatcg aaacaatgat tacccaagac atgaagtgag
    12061 ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt gttcgttact tcatttaata
    12121 gtaaagacaa aatgattggc atcggaaaaa acacgaagat atttaatccg aacaagatca
    12181 acgtatttct taataatgta aagaatgtcg gttctgtaaa caaacgaatg aaatgtttga
    12241 aaccgaccca aggacttccc attattccta aaaatggttg ataatcttga aaagcaataa
    12301 cgagtccccc cataggaaga tacctaaaaa tcaagaaata aaggactccc ggcagaatca
    12361 tcacatataa aaatttattc gttttgattc ctgcaattat tttttttcgt cttctgagct
    12421 tttcttcttt tttcgatagc gtcagttgaa atggttcttg tttctttaca ctttccataa
    12481 gcccctcctt agttttgtga atgtttgctg cttatgactt catgatagat aactatctga
    12541 attacgtata taatcatttt tatcgtaagt aaaaacgctt acatagcaat gatttttgtg
    12601 ctatacaaaa cgataaagtg tattttttca caaataaact acgcatagca aaccttctat
    12661 ttttttctat ctgacactta gcataggaaa tgattatata cgtaatcttt caccgatgat
    12721 aaattgaaca tatagaaaca gaaacattta atttattttg aggaggagcc tatctatatg
    12781 aaaaagaaag tctctatagg agaaagaagt tttacacttt tcaacaatac gtttctaatt
    12841 ttgctagctt tgatctgtat cgttcctttt ttaaatatca tcgctacatc ttttgcttct
    12901 acacaagaag tcgtcgcgaa aaagtttatc ctttttccta ctaccttttc tttagacgcg
    12961 tatcgctaca ttctttctac gccgaccatt tttagagcac ttgctgtttc gattggtgtt
    13021 acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac tgatggccta cggtctttcc
    13081 agaaagtatt tgtttggtcg aggtttcgtc aactttcttg ttgtattttc tatgttgttc
    13141 agcgggggga tgattccaac gttcttagtt gttcgttctc tagggctggt caattcttac
    13201 tgggccatga tcctgcctgt ggctgttaat gcaatgaata tgatcattat gagaaacttt
    13261 ttccaagcct tgccggatag tttggaagaa tcagcaaaaa tggatggttg cacagatttt
    13321 ggtgtatttt tcaaaattat gctgccatta gctttaccgt ctattgctac tatctcgctt
    13381 ttttatgctg ttacttactg gaacacttat atgactgcta ttttgtatat caacgattca
    13441 tctaaatggc cgatacagat tcttttacgc caaatcgtca ttgtatcaag tgggatgcaa
    13501 gcagaaagta gtgccgttga tgtgattcca ccagcacaaa cagttaaaat ggctgttatc
    13561 gttattgcaa cagtgcctat gctgattgtt tatccttttg ttcagaaata ttttgtcaaa
    13621 ggagcactcg taggttctgt caaagggtga ttatgtactg aacgcaaaat ggtagttgtg
    13681 tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg aaaattgctt cttaaatttt
    13741 tgtgaaattt cggcttattt ttcgaagaat ctactcctgg aacaccgtgt atcagaggtt
    13801 gtgaaagaga cgttcagcct tgagcgatga taaaaactct cacaaacatt gcgacgagta
    13861 acccacagga gcaataagaa gcgattttga aaaatagctt cccatatttc acaaaattat
    13921 gccttattgt cgatagcctg tctcttgaac gccatttatt cgtaaaaaag aactgtgaca
    13981 agacttttgt cacagttctt tttgcgtgct tagccttgaa atttaactat ccataaatac
    14041 aaatactagg gttattctaa cttcttttat tgcttgaatc agtccacaag aaaaacagcc
    14101 tcttgattaa acggcgttta tcttgttgat tctgcggcaa taaaatcagc cagcagcaaa
    14161 acatgaagtg ctgttttttg ctgtcctctt ttattgcttg aatcagatca caagaaaaac
    14221 agcctcttga ttcacaaata tttttttcga tattctcccg gcgtaatcga ttccttcttt
    14281 ttaaagaaac gaataaaatt ttgcggattt cgatattgca atctttctgc aatctctttt
    14341 acggagaggt tagtctcttt caaccatttt tttgctacct ctaaacgatg attttgaaca
    14401 aaatcaccaa atttttctcc tgtttccttt ttaaatacat tacttaaata gttagggtta
    14461 taatgcaacc tatcagcgat cacatctaaa gacagatcac gatcatattc tgtgtagacg
    14521 atatgcataa tattctccga caaattttta aattcatgat cagtgattgc ttgagagcat
    14581 tctgtgattg gcagaactaa ttgttctaaa acaagttttt ctagttcttt gggatgataa
    14641 ttgttcagtg cttttacata aatttgtttc atgttatcaa agttttttga atctactccc
    14701 aataactgtc ctaattgaac gagttcattg atcaagcgga ccgaaactac ttcccgactc
    14761 aaaggatttt tattttgtgc aaacaatgca tcaatcaatt catgtactaa attcttcact
    14821 tcctgatttc cagaacgaat ggcttcaaac aatcgatttt gttcttctat cggatatcta
    14881 ataagtgttt tttcatgagc agcaggaacg atttcctgat aaaaaataat tgaacttgga
    14941 ccagtattta cacgataata cagggcttct tttgcccgat caaccgattg cttgctttca
    15001 ttcagcgttt cgaaccggtt actgatgcct atactgatag ttaaattcaa gtattctttt
    15061 acacgttttt gaatgagtcg gcaatactcc atgacttttt tgcttgcttc tgcatcatct
    15121 ttatccattc gataaatagt ggcttgcatt tcttcattca aaacgatcgg cagcattcga
    15181 ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc
    15241 tccccaccgt gactatcttc taacagatcg atttgaacca aagctgtata atagcagtca
    15301 ttttgagaac tataaccaaa ttgctgcaac cgctgatcta gttctcgcct ttctactcga
    15361 tttctaaata aactaagtac aaatagcgtt tccagctgtg gcttttgggt aactaagtgt
    15421 gcacttaacg cttctttttc tcctatgatc cgactgactg attgagcgac caaatctagt
    15481 tctttcccac tgaatccttt tgatttaaga ttcagacgtt cctgtatttg agaaatcgga
    15541 cgggcaaatc tgtccgaaaa taagtaggaa aaaagaccaa tgagtcctac cagtgttaaa
    15601 gacactgcaa ctagcccgat tctcagattg cggatcattg cgccaattgc actagagtca
    15661 acttctaatc tgtaaatcca attattataa tctgatttca gtactatata cgttttatct
    15721 ttttctctta tcactttcag ctgattgtaa tctagatcca cgtccgttaa tacagttggt
    15781 tgctctttat tttttactgt ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa
    15841 agtatgtctt ctccactttc accaacaact tgttctattg tatgtttctc tatcgtagca
    15901 attcccaatg catatttttc tttttcaaac attggcaaag aaatcaccat ttcgattcct
    15961 ttgtttttct tttgccaaaa taaattattt ttgttattta tgtacttctg ttgatacttt
    16021 tcaacttcct cttcggtcag ttgactcaac gatccattga ttactcccca tttaccagcc
    16081 aaactgacta aatcataatt attatcatcc ataacaatcg tttctataaa atttagttct
    16141 ttcctgattt cagaataagt ggagtaatct gtatatgtca gcggatgctt gaatatgtcc
    16201 gagaaatttt tggtcgaacc atacgtagaa aacgaatatt caatcgttct aattttccat
    16261 tcaacgttag ttttaacttg cctgagcaaa ttctttttgt ctgttaaata actcgattcg
    16321 atcgattcat aggtattctg gtaaatgaat aaactaaatc ctatgacaag tacggctcct
    16381 aataaaaaca tcggtaaaaa tattttataa aatactgtac ttttcttcat tttcttcctc
    16441 tcctcctttt aaaataaaag gctttcattt ttctgtaaaa ttgatactca tctaattaaa
    16501 tgagcattcc ccgctttcaa gaatagcata attaaaaata aaaatccaag taatttttaa
    16561 cgtgtgcaat cttttcatat caaagatcta ttgacttatc gtagttatcg taatatgatg
    16621 aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg aatggtaacg atcaaagata
    16681 tcgctaaagc agctggcgtt tctcatacga cagtttctcg tgctttgaat aacagcagtt
    16741 taataaaaaa tgaaacaaaa caaaaaatac gacaactagc taaggaaatg aattattcgc
    16801 caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata tctaatcgga ctatttttct
    16861 caagtttgca taaaggaact tcatcaaatt tcctagcaga ttccattgca ggcatccatt
    16921 cagtattaga cacaaattac agtttatctg ttgaaagtat cgatgttgtt gatttaacag
    16981 aaatcaacct gcaacgctac gatggactga tcgttatgag tcaaagtgat gatgatcagc
    17041 ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca
    17101 atgatccagc ggttatcaac gtcactgccg atgatgcggc cggtgttatg caggcagtag
    17161 attacggtat tggatgcgga catacgaaga tcgcttatat tggcggaaag aaaaattttc
    17221 gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg
    17281 tcaatctagc ctatttttta acaggagatt acagcttaga aagcggcctc caaaacatgg
    17341 aatacctatt atcactttct gacctgccta ctctggtttt ttgcggcaac gacgacatag
    17401 cgattggtgc attgagagct gtacatttag ctggcctccg tgttccccag aacatttctc
    17461 tgatcgggtt cgatgattct ccagtcgtct cctatctcaa tccgccgctt accaccgtac
    17521 gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct tttactagat ttgatcgaag
    17581 gcaggacgat cgaacaatct aaaattgagc tctccacctt gttgatcctt cgtgagtcga
    17641 tcaattactt gtaaactaca agtaatttta tataaaaatg ttaacgtgtg cataacacag
    17701 aaaggaagtt tatatgaaaa aactgacaaa agaattagtg aaaaaaaacg aagcacctgt
    17761 aaaagttctt cagtttggtg aaggcaattt tatgcgtgga tttattgatt ggcagattca
    17821 gcagctgaat aaccaacaat tgtttaaagg taatgttgca atcattcaac ctttagaaca
    17881 aggtcttgga aatatgatga accagcaaga taatttatat acggtcattt tacaaggact
    17941 cagcaatggc gaaatcgttg atacttccga gattatcacc gtagtagaac aaatgatcaa
    18001 tccttatgat gaatgggatg cttatctatc tctagcagaa aatgacgact tggcctatat
    18061 cgtctccaat acaacagagg caggaatcca attcgtggag actgataatt taacagatac
    18121 tcccccacag agcttcccgg gcaaactgac agccttttta taccgacgtt tcaaattaaa
    18181 caaagctggc tttaccatca ttccttgcga actgattgac agaaacggcg aacgtctaaa
    18241 agaaatcatt ttacgctacg cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt
    18301 agaaaaagaa aatgttttct gttgtagttt agttgatcgc atcgtcccag gttatcctcg
    18361 cgaagatgca ggtgttttca atcaacggca taactatgaa gatcaattga tggtcaaagc
    18421 agaaccattc atgctttggg tgatcgaagg gcctaaagaa ttggagaatc aactaccatt
    18481 caaacaggct ggcttgaatg tcatcatcac agatgatatg actccttatc gtgaacgtaa
    18541 ggtgcatttg cttaatggac cgcatacagc gatggtccct cttgctttgt tggcggaatt
    18601 ggaaactgta gaagaagtaa tgaaagatcc tttattctcc gcatatgttg atcagttatt
    18661 caatctagaa ttgattccta tgctttcttt acctaaagat gaattagcca tttatgctga
    18721 ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac aaattagaag cgatttcatt
    18781 aaatagtgtt tcaaagttca gcacccgtct gcttccagtg ttcaagaagt atatcgaaga
    18841 acaaaatcag gtaccaccgt taatcactgt ttcactagct gcgctacttc ttatgtatcg
    18901 aggcgatcaa gtaaagccac atgatgatga aaagacgata agcgaattta ctgatgcttg
    18961 gtctgataat ggaacagcta ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga
    19021 tttgtctcaa ataccgaatg tgacagatac agttcaagaa atagctgcaa agatcgatca
    19081 aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg atggtccatg cttgaaaccc
    19141 tgcaaccaac aatgaaatta aatcgacgag acagtgtggt cgtcgcatta acccctattc
    19201 caaaacaaac acaactgacg attgatcagc aaacgatcac cactcttgaa gatatcccgc
    19261 aaggtcataa gattgcattg ggtgatttga aagctggaga caacgtgatc aaatatggct
    19321 atccaatcgg tcatgtgacg acagacgtaa ctgccggtca atggctgcac actcataatg
    19381 taaaaaccaa tctttctggt gaattggatt atcgatatga aaaagatgtc catccaagtc
    19441 attacctttt tgagaaccga acatttcaag gttacttgcg aaaaaatgga aaagtaggga
    19501 ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt gaatggcatt gctgaactga
    19561 tcgtcaaaca gttcaaggaa aaacatccag atctgggaca gtttgaccat attacgattt
    19621 taaagcatcc ttatggctgt tcgcagcttg gaaaagatca tcaaaatact cgagaaattt
    19681 tagcggacgc agtaaatcat cccaatgccg gaggagtttt agtatttgga ttaggttgtg
    19741 agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga
    19801 aatttctagt ggctcaagat gtttacgatg agatcgaaca aggtgtggct ctattagaag
    19861 agcttttaac agcggcggaa aatgaccagc gcatatcagt cccgctctct aagttgaatg
    19921 tcggtttgaa atgcggcggt tctgatggtc tatcgggaat aactgctaat cctttacttg
    19981 gtgcattttc tgactacttg attgctcaag gaggcagcac gatcttaaca gaagtgccag
    20041 aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga aaatgaagaa gtttttgact
    20101 caatcgttga cttgatcaat gacttcaaac agtatttctt gtcttacggg gaaccagttt
    20161 acgaaaaccc atctcctggt aataaagcag gtgggatcac tacattagaa gataagtctt
    20221 taggctgcac gcaaaaatca ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa
    20281 aaatcagaaa accagggctt agtttattgc aagctcccgg gaatgatctt gtagctgcct
    20341 ctgctcttgc ttcatccgat tgccagttgg tcttgtttac cacaggaaga gggactccgt
    20401 ttggctcata tgtcccaacg gtcaaggtat ctacgaatac aactcttttt gaacgcaaag
    20461 gtcattggat ggattttaat gcaggcgtct tattagaaca accaatggaa gtaatcttag
    20521 aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa
    20581 atgacgttcg agaaattgcc atttttaaaa acggagtaac actttgattt tcataaaaca
    20641 gtataaaaag aaagaaggaa gatttatgtt tttatctgat gattttttat taaaagacga
    20701 ttgggcgaaa aagctttacc attcctacgc aaaaaacatg cccattattg attatcactg
    20761 tcacttagat ccaaaagaaa tttacgaaaa caacaatttc agcaatctga cagaagcttg
    20821 gttaggaggc gatcattaca aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa
    20881 aatcacagga aatgcttctg attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa
    20941 aattatcgga aatccgcttt acagttggtc acatcttgaa ttgaagcgtt tcttcggtat
    21001 tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt
    21061 ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg
    21121 tacaacagat gatcccgcag acgacttgca ctatcatcag ttgcttgctc atgaagaacc
    21181 agatttgaag gttctacctt cattccgtcc agataaagca ctcaatattg aaaaagaagg
    21241 atttaatgca tgggtcacac tactggaaga agcagctgac caaacgatca ccacctatca
    21301 agaactgata gaagtcttag ggcagcggat agaatacttc catcaacatg gatgtcgtgt
    21361 ttccgaccac gctttagaca tcttacgcta taaagaagcg gatgaaacag tattagaaga
    21421 gatttttcaa aaagcacgag ctaacaaact attatcttca gatgaaatcg atgcttatct
    21481 tacagaaaca ctgatacatt tgacccactt ctatcattct cataattgga cgatgcagct
    21541 gcacatccat gcttatcgta actgtaatac acaaatgttg gaaaaattag ggcccgatac
    21601 cggctatgat ggaatgaatg atctttcttt gactattcct ctgcaaaaat tattgaaccg
    21661 tgcagaagaa acggatcagt tgccaaagac gatcctttat tcattgaatc cgaatgacta
    21721 tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt
    21781 aggttctgga tggtggtaca acgatactcg agctggtatg cgcgatcaat tgacacaatt
    21841 tgctgatggt agtgctttgg ggaattttgt cggcatgctg acagattcaa gaagtttcct
    21901 gtcctacact agacatgaat atttccgtcg tgttctttgt gaatttttag gcgaaatggt
    21961 catgcgagga gaagcacctg aagatgaaca acttttagga agtctagttc aagcgattag
    22021 ttatacaaat gctaaaaatt actttggctt ttttgcctaa ttgattaata gtaacagtaa
    22081 cgaatcaaaa acaggatagt gaaataacgg aatccgttta tccacgtttt agagaagaag
    22141 acggaccaa
//
LOCUS       DARBEG010000041        21988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000041 DARBEG010000000
VERSION     DARBEG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.041"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCK20_001552"
     CDS             <1..395
                     /locus_tag="SCK20_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145607.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SCK20_001553"
     CDS             398..1147
                     /locus_tag="SCK20_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145608.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SCK20_001554"
     CDS             1159..1428
                     /locus_tag="SCK20_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145609.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SCK20_001555"
     CDS             1433..1597
                     /locus_tag="SCK20_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145610.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SCK20_001556"
     CDS             1590..1892
                     /locus_tag="SCK20_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145611.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SCK20_001557"
     CDS             1889..2050
                     /locus_tag="SCK20_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1145612.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SCK20_001558"
     CDS             2047..2352
                     /locus_tag="SCK20_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145613.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SCK20_001559"
     CDS             2352..2708
                     /locus_tag="SCK20_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG1145614.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SCK20_001560"
     CDS             2695..2913
                     /locus_tag="SCK20_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145615.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SCK20_001561"
     CDS             2910..3329
                     /locus_tag="SCK20_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1145616.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SCK20_001562"
     CDS             3326..3883
                     /locus_tag="SCK20_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1145617.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SCK20_001563"
     CDS             3880..4176
                     /locus_tag="SCK20_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145618.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SCK20_001564"
     CDS             4253..4666
                     /locus_tag="SCK20_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1145619.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SCK20_001565"
     CDS             5012..5386
                     /locus_tag="SCK20_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145620.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SCK20_001566"
     CDS             5423..5590
                     /locus_tag="SCK20_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145621.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SCK20_001567"
     CDS             5616..5960
                     /locus_tag="SCK20_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1145622.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SCK20_001568"
     CDS             5965..6246
                     /locus_tag="SCK20_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145623.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SCK20_001569"
     CDS             6349..6663
                     /locus_tag="SCK20_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG1145624.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SCK20_001570"
     CDS             6641..8335
                     /locus_tag="SCK20_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1145625.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SCK20_001571"
     CDS             8355..9533
                     /locus_tag="SCK20_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1145626.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SCK20_001572"
     CDS             9496..10182
                     /locus_tag="SCK20_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1145627.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SCK20_001573"
     CDS             10182..11342
                     /locus_tag="SCK20_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1145628.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SCK20_001574"
     CDS             11352..12227
                     /locus_tag="SCK20_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145629.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SCK20_001575"
     CDS             12224..12535
                     /locus_tag="SCK20_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145630.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SCK20_001576"
     CDS             12525..12878
                     /locus_tag="SCK20_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1145631.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SCK20_001577"
     CDS             12868..13269
                     /locus_tag="SCK20_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145632.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SCK20_001578"
     CDS             13262..13666
                     /locus_tag="SCK20_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145633.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SCK20_001579"
     CDS             13678..14286
                     /locus_tag="SCK20_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1145634.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SCK20_001580"
     CDS             14306..14668
                     /locus_tag="SCK20_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145635.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SCK20_001581"
     CDS             14671..14853
                     /locus_tag="SCK20_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145636.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SCK20_001582"
     CDS             14870..18289
                     /locus_tag="SCK20_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1145637.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SCK20_001583"
     CDS             18340..19077
                     /locus_tag="SCK20_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1145638.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SCK20_001584"
     CDS             19087..21378
                     /locus_tag="SCK20_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1145639.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SCK20_001585"
     CDS             21402..>21988
                     /locus_tag="SCK20_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145640.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARBEG010000042        21912 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000042 DARBEG010000000
VERSION     DARBEG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.042"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCK20_001586"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCK20_001586"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG1145641.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SCK20_001587"
     CDS             complement(1172..1867)
                     /locus_tag="SCK20_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG1145642.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SCK20_001588"
     CDS             complement(1880..2596)
                     /locus_tag="SCK20_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG1145643.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SCK20_001589"
     CDS             2926..3534
                     /locus_tag="SCK20_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG1145644.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SCK20_001590"
     CDS             3859..4182
                     /locus_tag="SCK20_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG1145645.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SCK20_001591"
     CDS             4364..5734
                     /locus_tag="SCK20_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG1145646.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SCK20_001592"
     CDS             complement(5864..7165)
                     /locus_tag="SCK20_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1145647.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SCK20_001593"
     CDS             complement(7519..9477)
                     /locus_tag="SCK20_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG1145648.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SCK20_001594"
     CDS             complement(9654..9920)
                     /locus_tag="SCK20_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1145649.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCK20_001595"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCK20_001595"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG1145650.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCK20_001596"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCK20_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG1145651.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SCK20_001597"
     CDS             13264..13827
                     /locus_tag="SCK20_001597"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1145652.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SCK20_001598"
     CDS             complement(14080..14601)
                     /locus_tag="SCK20_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG1145653.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SCK20_001599"
     CDS             complement(14604..15446)
                     /locus_tag="SCK20_001599"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG1145654.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SCK20_001600"
     CDS             complement(15439..16164)
                     /locus_tag="SCK20_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG1145655.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SCK20_001601"
     CDS             16340..16636
                     /locus_tag="SCK20_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145656.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SCK20_001602"
     CDS             17204..18796
                     /locus_tag="SCK20_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1145657.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SCK20_001603"
     CDS             18809..19549
                     /locus_tag="SCK20_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1145658.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SCK20_001604"
     CDS             19717..21066
                     /locus_tag="SCK20_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1145659.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SCK20_001605"
     CDS             complement(21194..21784)
                     /locus_tag="SCK20_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1145660.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DARBEG010000043        21396 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000043 DARBEG010000000
VERSION     DARBEG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.043"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            77..778
                     /locus_tag="SCK20_001606"
     CDS             77..778
                     /locus_tag="SCK20_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1145661.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1387..2403)
                     /gene="tsaD"
                     /locus_tag="SCK20_001607"
     CDS             complement(1387..2403)
                     /gene="tsaD"
                     /locus_tag="SCK20_001607"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG1145662.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2400..2864)
                     /gene="rimI"
                     /locus_tag="SCK20_001608"
     CDS             complement(2400..2864)
                     /gene="rimI"
                     /locus_tag="SCK20_001608"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1145663.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2871..3413)
                     /gene="rimI"
                     /locus_tag="SCK20_001609"
     CDS             complement(2871..3413)
                     /gene="rimI"
                     /locus_tag="SCK20_001609"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1145664.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3397..4119)
                     /gene="tsaB"
                     /locus_tag="SCK20_001610"
     CDS             complement(3397..4119)
                     /gene="tsaB"
                     /locus_tag="SCK20_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG1145665.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4310..5290)
                     /locus_tag="SCK20_001611"
     CDS             complement(4310..5290)
                     /locus_tag="SCK20_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1145666.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5314..6798)
                     /gene="galT"
                     /locus_tag="SCK20_001612"
     CDS             complement(5314..6798)
                     /gene="galT"
                     /locus_tag="SCK20_001612"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG1145667.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6810..7799)
                     /gene="galE"
                     /locus_tag="SCK20_001613"
     CDS             complement(6810..7799)
                     /gene="galE"
                     /locus_tag="SCK20_001613"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG1145668.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8047..8214
                     /locus_tag="SCK20_001614"
     CDS             8047..8214
                     /locus_tag="SCK20_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG1145669.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8276..10087)
                     /locus_tag="SCK20_001615"
     CDS             complement(8276..10087)
                     /locus_tag="SCK20_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG1145670.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10084..10449)
                     /locus_tag="SCK20_001616"
     CDS             complement(10084..10449)
                     /locus_tag="SCK20_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG1145671.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10612..11007)
                     /locus_tag="SCK20_001617"
     CDS             complement(10612..11007)
                     /locus_tag="SCK20_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1145672.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11025..11987)
                     /locus_tag="SCK20_001618"
     CDS             complement(11025..11987)
                     /locus_tag="SCK20_001618"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG1145673.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11987..12199)
                     /locus_tag="SCK20_001619"
     CDS             complement(11987..12199)
                     /locus_tag="SCK20_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG1145674.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12220..12918)
                     /locus_tag="SCK20_001620"
     CDS             complement(12220..12918)
                     /locus_tag="SCK20_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG1145675.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12938..13480)
                     /locus_tag="SCK20_001621"
     CDS             complement(12938..13480)
                     /locus_tag="SCK20_001621"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG1145676.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13612..14616)
                     /locus_tag="SCK20_001622"
     CDS             complement(13612..14616)
                     /locus_tag="SCK20_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1145677.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14613..15602)
                     /locus_tag="SCK20_001623"
     CDS             complement(14613..15602)
                     /locus_tag="SCK20_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1145678.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15599..16405)
                     /locus_tag="SCK20_001624"
     CDS             complement(15599..16405)
                     /locus_tag="SCK20_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1145679.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16571..17527
                     /locus_tag="SCK20_001625"
     CDS             16571..17527
                     /locus_tag="SCK20_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1145680.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17604..18122)
                     /locus_tag="SCK20_001626"
     CDS             complement(17604..18122)
                     /locus_tag="SCK20_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1145681.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18210..18359)
                     /gene="rpmG"
                     /locus_tag="SCK20_001627"
     CDS             complement(18210..18359)
                     /gene="rpmG"
                     /locus_tag="SCK20_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1145682.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18587..19033
                     /locus_tag="SCK20_001628"
     CDS             18587..19033
                     /locus_tag="SCK20_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG1145683.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19228..21123)
                     /gene="asnB"
                     /locus_tag="SCK20_001629"
     CDS             complement(19228..21123)
                     /gene="asnB"
                     /locus_tag="SCK20_001629"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG1145684.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6433 a         4547 c         3695 g         6721 t
ORIGIN      
        1 tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata gtgaacaata
       61 aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata
      121 tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt
      181 tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt
      241 gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta
      301 ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga
      361 gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc
      421 ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat
      481 ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt
      541 tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga
      601 tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac
      661 gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta
      721 tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga gagaataacg
      781 cgaacaaaaa taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta
      841 aaacttctga aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt
      901 gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa
      961 gcgtcttgtc ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca
     1021 gtgagggcaa tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca
     1081 ataatgatta aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac agccctattt
     1141 cgattaaacg gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg
     1201 aggagctgtt ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact
     1261 cttgattaaa cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa
     1321 catgagaagc cgtttttcct gggctgttct tattacttga tgcagggcag ctttttcaca
     1381 acctctttat tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt
     1441 tcttttttgc atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag
     1501 tcgaagaggg ggaatcacta gttctacctc agggagttta gctgccaaag catcctgcaa
     1561 tccttcacgc aacccttgat tggccgctac acctccagct ataatcagtt gcttgactgg
     1621 ataattttga caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa
     1681 gctagctgct aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa
     1741 attgataaat gcacttttta atccgctaaa actgaaatca taattatctt catggatcat
     1801 cgctcgcgga aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc
     1861 gcttgggtaa gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc
     1921 atctcttgtc tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt
     1981 atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc
     2041 tgcatagata tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc
     2101 aaaagcctta gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt
     2161 gacagcgact gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa
     2221 gcataaggtg atttgttcta cgtgatggcg gctagctact tcaggtacta cgccaccaaa
     2281 acgtttatga ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct
     2341 aacgaccgct acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt
     2401 catttcatcg tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg
     2461 cgataatatg atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt
     2521 gctgcttcat tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc
     2581 gtaatcagta attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca
     2641 gcaatatttg tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc
     2701 tggttatctt cagctaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac
     2761 tgcattttcg tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat
     2821 tctgccaagt acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt
     2881 tctaaaagtt ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg
     2941 atcgttgttg cttgaaaccc caatttccga taaagtcttt gtgcatcttg attactcata
     3001 cgcacttcta atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca
     3061 ataagcagcg aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata
     3121 tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag
     3181 atacctagat atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc
     3241 catggcaatt cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct
     3301 gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt cgactgtttt ttctgtataa
     3361 atcattttat cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc
     3421 gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt ttaaatagcg
     3481 aggaagaaaa gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt gagccacagt
     3541 tacaccactt ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc
     3601 ctttatttca gagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag
     3661 cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacagtttc
     3721 taatctattt tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa
     3781 catgggcaca ataatattat ccacaccgat gcaattagcc gcgatggttt tcagactaga
     3841 tactcctact agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat
     3901 acgaagccct gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg
     3961 acttatttga agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt
     4021 gcgtttgata tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc
     4081 caaagtttgg tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat
     4141 gacattctcg ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc
     4201 aaaactagta caataataca tgattatctg ctggcgggca aattttgccc cttcctgaaa
     4261 cacaaaaaag ctgcggtatc actccatttt cccacagctt ttccatcttt tatttcgttg
     4321 attctcgaat aatcaactct gtttccacaa taatctttct tgaaaccggt gctttggaat
     4381 tacttaattg aagaatcgtc tccactgcta gctctcccat ccattcggta taaactttta
     4441 cagtagaaag cgagggcgtt acatatttcg ctacactgac atcattgaag cccatcacag
     4501 aaatatcttc tggtaccact aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta
     4561 atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg attcgttttt aaatattgct
     4621 tcactgcttg atatcctgac tcgacagaaa aatctgcttc gataattgga ccttcgtcgt
     4681 agcctttttc tgccaaaact tgttgaaatg ccataattct gggatctttt agccgctgat
     4741 ggtttttctt cgtatattct gttccagcaa gcatagcgat atgttgcgct ttatgggaga
     4801 caaagtgttc aatgactttt tgaacacttg acttaaagtc gacaactaga gagtcgattc
     4861 cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta
     4921 ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga agatcactaa
     4981 tggaatccca cgtttctttc aataaagtaa cgtttgcttc ttctgccttt ttttcgattc
     5041 ctaggcgaat agataaataa taaagatctt caagctcttc cgtatcgtcg taccactgga
     5101 tcaggcgaag cgtttgctta gtatttgctg catttttacg atgtttcgta taatttaagc
     5161 gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga tcaggatcat
     5221 agttgagaac tcttgaaaca gtagcaggag aaactcctgc caactgcgca atgtctttaa
     5281 ttgttgccat ttgtctctcc tcctttttta cgtttataaa atcgcagtaa atcgttcaaa
     5341 cgctgcctgt cctttttcat ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa
     5401 aacctgacct acagattttt gtacgacatc ggtgaccgta tctttcgtca aatcaggatg
     5461 ttcttttttt aattgttctg cccacggacg atgatattca gccatctggt tttcttctcc
     5521 taataagaaa tgctctactt ctactagttc tggtttcaaa cgaggtggta aaacagcaag
     5581 ccccattact tcgatcaacc caatgttctc ctttttgata tgttggacat ctttatgtgg
     5641 atgaaaaatc ccatcaggat gttcttctga aacattgttg tctcgaagga caaggtccaa
     5701 ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc
     5761 tgctgaaaaa gctctgatag atactgtttc atccgaatat gctttccatt tttccaatac
     5821 tgagttagct gcttcaataa gcaagttttt ctctgttgat tgcaaacgga tcacagacat
     5881 cggccatttc acgactcctg cgatgacttg cggataatct tctagtttga taagtttttc
     5941 tatcggtgct tttgccatag gaaattcatg acgtcctgcc tgataatgat catgagaaag
     6001 aatcgatcct ccaacaattg gtaagtcagc attcgaacca acaaagtaat gaggcaagac
     6061 ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg
     6121 ttctgataaa atgatcgaat gttcattgta ataagcataa ggggaatatt ggaatcccca
     6181 gctttcaccg tctagattca ttcgaataat ccggtgattg gtgcgggctg gatagttcaa
     6241 gcgacctcta tatccttcgt tttccatgca aagcatgcag gtaggatagt ctacttttac
     6301 cgcctcgcgt tctgctgcga tttgttttgg atctttttct ggcttagata aattgattgt
     6361 gatttccagt tcaccatact ccgactctgc cggaaatttg atgttttttg cgatggctct
     6421 tgttttgatg tagtcatttt tcttacttaa attaaagaaa tactcagttg cttcctctgg
     6481 gcttttcgaa tagtgctgtg caaagaatgc attgacgact gaaggtggcg gtgtgagaaa
     6541 atccattaat tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa gaacaccatt
     6601 ttctcttgcc ttagcgacaa gctgatccaa taagtctaca gattcttcct taactggtct
     6661 tacttcaatg ctttctagag catcttctcc taccatccct aaaagacgat tctgtaaata
     6721 taagcggtcc atttccatcc atccgccagc ttcgatcgca agtgtggcaa aatcacaaat
     6781 cgtttgactg attgacatat ctcattcccc tattcttcat aaccatttgg atggctgaca
     6841 tgccaatccc atgctgtttt gataatcgct ttgatatctg tgtattcagg tttccagccc
     6901 agaatctctc tcgctttttc acttgaagcg actaatcggc taggatctcc agctcttctt
     6961 ggtgctattt ctgctggaat ttcttttcct gtcacttctc tagctgcttc tagcatttcc
     7021 ttgactgagt atcctttgct gctgcctaaa ttgaagaagt tgctttcatt tccttctttc
     7081 aagtattcca aagctaaaat atgtgcagca atcaaatctt ctacttgaac ataatctctg
     7141 atacatgttc catctggtgt atcataatcg tctccataaa cagccaaggc ttttctttga
     7201 ccaagagcta cttgtaaaat gattgggaca agatgcgttt ctggtgtatg atcttctccg
     7261 atagatgcat ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac atagcgcatg
     7321 ccatatgctt ggtcacacca cttcatcatt ttttccatca tcaatttact ttcaccatac
     7381 ggatttttag gattagctgg tgtatcttct gtgattggag actctttcgg ctctccataa
     7441 gttgctgcag tagaagagaa tacgatttta ttcacgttga actcgtgcat gacttcaagt
     7501 aagatttgca taccgtatac gttgttgttg aaatacatca atggtttttc gacagattct
     7561 cctactaaag agcttgcagc aaaatggatt actccttcaa tcggttcctt ttcaaatacg
     7621 gaacgtagaa attctttatc ccggatgtct ccttcataaa aatgagcatc tggatgtact
     7681 gcttgtttgt gacctgtgag caaattatct accacagcta cttgataccc cttttgaact
     7741 agctggtcta cagcgtgcga accgatataa ccggctccgc ctagtactaa aattgtcata
     7801 aaaaacactc ctttgattag taaaattgtg tttgttgatt ctatataatt taaaaaacga
     7861 agtttacttt tatggtgaaa acaaacaaag taaaatttcg taaattcata tggtaaattt
     7921 ataataaaac atttagtaaa cattttcaag ggttttttgc aaaggtttac aaacaaacgt
     7981 aaaactagcc tttttttctc ttttgttcca taatagatat aacaaataat aaatgaggtg
     8041 gattcaatga aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc
     8101 gttgctggtt tggttgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa
     8161 gcaatgatcg aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg ttaataatta
     8221 tttaaaaata cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt
     8281 tgtcagtcaa tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat
     8341 aaggtctagc tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat
     8401 aaaagtcaga ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat
     8461 ctatagcttc tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt
     8521 tgttttcctt caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca
     8581 atcgagcttc aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc
     8641 ggttgtgctt ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta
     8701 tcagtgctgc taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta
     8761 gatgagtaaa ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt
     8821 caatagcctg ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg
     8881 ctcctgtgca attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag
     8941 cttctgaaaa gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc
     9001 tataagggat tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat
     9061 catgctcaaa atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat
     9121 aaaacaactc aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg
     9181 actgatgcca ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgctccgc
     9241 cttgcttttg atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata
     9301 caatcggttc cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc
     9361 ctcctcttga tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta
     9421 aaaagacatt ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa
     9481 gctgatacag taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct
     9541 catcattttc ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat
     9601 ctgtctgtaa aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa
     9661 actgaataga agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc
     9721 cgtataaaca tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta
     9781 gttgcttatt tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt
     9841 gaaacaagtt gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa
     9901 gcgtcacttg ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa
     9961 ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat
    10021 tattatggag aatcctttct gctacactta atcctctagg tccggcacca atgatcccta
    10081 ttttcatttg catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg
    10141 ttcatttttt gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag
    10201 tttaaaaata gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc
    10261 aaatgaagca aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc
    10321 aatttctttc aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa
    10381 tctatccacg agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa
    10441 tttgatcata gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata
    10501 gcacaaaata aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt
    10561 caaaatctaa tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt
    10621 tttacttttg atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg
    10681 ataacctgct ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta
    10741 aatatagaca ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg
    10801 gatgtttctt gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat
    10861 tacttgtgct tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg
    10921 tttggccatt actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa
    10981 aatgatatta attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag
    11041 gctaatgagg cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt
    11101 gttgattctc ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt
    11161 aaaaattctc ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta
    11221 ttccccaagt ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat
    11281 gcaaacggat cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg
    11341 ttgtctagca ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct
    11401 aggcgtacca cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta
    11461 cattcgaacc cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg
    11521 ccgtgaatca aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg
    11581 aatacaacgt ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca
    11641 ttcgcgtcat tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca
    11701 aattgaactt ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc
    11761 acgcttcctg gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa
    11821 ttcaaacgat gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa
    11881 atattggtat cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat
    11941 tttgctgttg ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt
    12001 tctttcggct ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg
    12061 tatcatgatc aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa
    12121 tgtcatagat gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta
    12181 catcatataa gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt
    12241 ttttaaaccc agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat
    12301 cctttggtgt atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac
    12361 taatccgcca attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc
    12421 aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg
    12481 gttatgttta aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt
    12541 acttgcacca ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat
    12601 tatcccgctg aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg
    12661 ctgccccata aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat
    12721 gaagattggt gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat
    12781 tccggcataa taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt
    12841 ctgtatccct aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat
    12901 ttgctgttga taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga
    12961 tcttctgatc aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc
    13021 tacatgtttt tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga
    13081 ttcgataacc ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg
    13141 gcaaatagag cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg
    13201 actgttcatc catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta
    13261 ttttcttttg ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat
    13321 tcgataaagt cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt
    13381 tatagattcg ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt
    13441 cgattccttt ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct
    13501 cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa
    13561 tagaataaaa agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt
    13621 cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc
    13681 aatcaaaatt cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc
    13741 actaagcaga aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt
    13801 acgtacgact agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt
    13861 caccgctaca ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt
    13921 agccacgtta aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct
    13981 ctgtgcataa agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac
    14041 ataaggccag ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt
    14101 ttccttagat aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc
    14161 aataccggac aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag
    14221 atagacacag caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg
    14281 cagcgaatag tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt
    14341 aatgcccaat atactagaat ctgccaattc atttcttgtt actccttgca agatataacc
    14401 agaaacgcca agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc
    14461 tctaacaatc agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc
    14521 caggtaaata aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa
    14581 aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc
    14641 agtaaaaacg gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga
    14701 ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag
    14761 acaggtagaa cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac
    14821 agcccaataa atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact
    14881 agacagaaaa caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt
    14941 tcactcaaat gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt
    15001 atatataaag gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca
    15061 aaccaaaaag caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca
    15121 gtaaaaaaag aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt
    15181 ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac
    15241 attagaaaca cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc
    15301 aacgaagcac ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcacc
    15361 ccttgcatca atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca
    15421 cgaggcaaac gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac
    15481 aacgcctgcc aaaaatctgc tttttccaca gtcgctgcgc catttgaaag agcaacaaaa
    15541 aaaataatca aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc
    15601 ataattgatc ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg
    15661 ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg
    15721 tgatcacttc ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat
    15781 aatctgaaaa acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt
    15841 tcatttgatt gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa
    15901 atgttgtcgg ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc
    15961 agacacgctg ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta
    16021 gacctgtagc atccaacgcc cactcaattg cttcatgatc ttgcgtgtcc atgccactga
    16081 acagtttttg ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag
    16141 ggtgttcact agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg
    16201 tagagatatc agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa
    16261 aacttttcaa taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc
    16321 cttcaggaat cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa
    16381 tgtcagctgc tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt
    16441 atgacgctgt ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg
    16501 ttgacagcgt tcgaaatatc aggaataata accaatgaga aagattctca attatgttag
    16561 gaggaaagaa atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc
    16621 tggagtactg agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc
    16681 gacgacttca tccaatagta ctcatacagt cacagataca cttggacata aagtagagat
    16741 tccaaacgcg cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg
    16801 ggaaaaacct atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga
    16861 tgatttaaag gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt
    16921 tgagcctgat ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca
    16981 atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga
    17041 acaactaaaa gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc
    17101 cgattatcaa aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa
    17161 gacagctgca gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc
    17221 aagtggaaga atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt
    17281 ctccaaaaaa gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga
    17341 tgctgactac atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag
    17401 ctggaagaat ctaaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag
    17461 ttggctatat aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact
    17521 aaattgattt taactaaaaa aagcatcttc acgattagtc gtgagatgct ttttttctta
    17581 ttaacaaact agacgcatag attttattct tcttttgaag caaccctatt ttttactctt
    17641 tcccattttt gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc
    17701 tctataaggg cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg
    17761 ctatcgatca atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga
    17821 ctaccacccg aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg
    17881 atcgaatgca ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg
    17941 atgatatggc gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc
    18001 actaaaattc gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat
    18061 ggtaacaaac catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac
    18121 atattatccg ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga
    18181 ccagcaagga attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg
    18241 cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg
    18301 cttgttagat agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg
    18361 tttttccctc ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac
    18421 catgattttt tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg
    18481 ataaatacaa cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta
    18541 cacttgttta tatagattat gttttttaat aaagggggat actaatatgc catggaacat
    18601 gaaggacttt cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga
    18661 catcgcaaat gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat
    18721 tgatcaagca aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa
    18781 agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct
    18841 tgattcagat gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc
    18901 aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt
    18961 agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga
    19021 aatcgattat tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat
    19081 gttcgaaaca atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac
    19141 gggaagagct gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac
    19201 ctctttttta ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg
    19261 cggaatcgtt tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca
    19321 gatttttctt tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt
    19381 ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta
    19441 gccgtgttct tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt
    19501 cgaccattct tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt
    19561 ttgattcaat aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa
    19621 taaaggtact cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat
    19681 atcttttggc atccattgat gaagatcgac atattgcatc ttattgactt catctttgat
    19741 tccttctgtt ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata
    19801 ttttggtgtt aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa
    19861 atattctttt gcaggagcaa gtgattcata taaatggatc cgtccatgga agtttttcat
    19921 ttttccgatt ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg
    19981 agcaatcaca cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa
    20041 cagttcatcc gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc
    20101 taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat
    20161 caaagggaat gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc
    20221 caatagttcg gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc
    20281 gattgagaaa gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct
    20341 ggataagaaa gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac
    20401 ggtttcgtcg attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc
    20461 tgcatcccaa tattgctgaa tgtctatttt tccatcttta tacgtataat aatggccttc
    20521 aggcaaacga tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt
    20581 catataaggt ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa
    20641 acttttgatt tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt
    20701 gatcccaaag tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa
    20761 agcaaacatt ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa
    20821 aaggacttct gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct
    20881 caaaggctgg taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt
    20941 gtaaataggc tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc
    21001 aacatgttta tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat
    21061 cattgtatta ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca
    21121 catttaactt tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc
    21181 atagaataaa tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag
    21241 atgaaagttt ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt
    21301 ttttgaggct accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa
    21361 aaaccagcaa aaaaagacac gacctaaaga tcgtgt
//
LOCUS       DARBEG010000044        20586 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000044 DARBEG010000000
VERSION     DARBEG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.044"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="SCK20_001630"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="SCK20_001630"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG1145685.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="SCK20_001631"
     CDS             1011..2258
                     /locus_tag="SCK20_001631"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG1145686.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="SCK20_001632"
     CDS             2334..2678
                     /locus_tag="SCK20_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145687.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="SCK20_001633"
     CDS             complement(2795..3121)
                     /locus_tag="SCK20_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145688.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3736..4938)
                     /locus_tag="SCK20_001634"
     CDS             complement(3736..4938)
                     /locus_tag="SCK20_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1145689.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5055..5570
                     /locus_tag="SCK20_001635"
     CDS             5055..5570
                     /locus_tag="SCK20_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1145690.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5769..6215)
                     /locus_tag="SCK20_001636"
     CDS             complement(5769..6215)
                     /locus_tag="SCK20_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1145691.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="SCK20_001637"
     CDS             complement(6262..7740)
                     /gene="msr(C)"
                     /locus_tag="SCK20_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1145692.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8094..8723)
                     /locus_tag="SCK20_001638"
     CDS             complement(8094..8723)
                     /locus_tag="SCK20_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1145693.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8707..9171)
                     /locus_tag="SCK20_001639"
     CDS             complement(8707..9171)
                     /locus_tag="SCK20_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1145694.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9658..10926)
                     /locus_tag="SCK20_001640"
     CDS             complement(9658..10926)
                     /locus_tag="SCK20_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1145695.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11157..11894)
                     /locus_tag="SCK20_001641"
     CDS             complement(11157..11894)
                     /locus_tag="SCK20_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1145696.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11961..12530)
                     /locus_tag="SCK20_001642"
     CDS             complement(11961..12530)
                     /locus_tag="SCK20_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1145697.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12658..13530
                     /locus_tag="SCK20_001643"
     CDS             12658..13530
                     /locus_tag="SCK20_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1145698.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13872..14744
                     /locus_tag="SCK20_001644"
     CDS             13872..14744
                     /locus_tag="SCK20_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1145699.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14741..16699
                     /locus_tag="SCK20_001645"
     CDS             14741..16699
                     /locus_tag="SCK20_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1145700.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17031..17882)
                     /locus_tag="SCK20_001646"
     CDS             complement(17031..17882)
                     /locus_tag="SCK20_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1145701.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18005..18472
                     /locus_tag="SCK20_001647"
     CDS             18005..18472
                     /locus_tag="SCK20_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG1145702.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18735..19217)
                     /locus_tag="SCK20_001648"
     CDS             complement(18735..19217)
                     /locus_tag="SCK20_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG1145703.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19317..20180)
                     /locus_tag="SCK20_001649"
     CDS             complement(19317..20180)
                     /locus_tag="SCK20_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1145704.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         6197 a         4227 c         3724 g         6438 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat
     3421 atgagaagct atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca
     3481 acctctgtta aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa
     3541 tatgaggaac tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca
     3601 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
     3661 aaatatgaga agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc
     3721 acaacctctg atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat
     3781 acaacataca ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat
     3841 atgttcacca atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa
     3901 actatagctg gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt
     3961 caacagtgta ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag
     4021 gactggcata gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc
     4081 tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga
     4141 agcaatagcc agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata
     4201 gccaatcaat aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa
     4261 tgcgattagt ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag
     4321 gcctactttt ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc
     4381 gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac
     4441 cccaccgact agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat
     4501 tgctttttct gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa
     4561 taaagcattc ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga
     4621 taatccgcca agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt
     4681 caaaccgatc cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat
     4741 gtaactagta aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt
     4801 ttttaagatt gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac
     4861 aactagcgct gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg
     4921 attctccaat atttccatcc ctttctctct attagcatca tacatgattt attttacatg
     4981 tcaatatgca catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga
     5041 gaggtaggat ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac
     5101 cacgttctgg gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc
     5161 agatctatac aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag
     5221 acgagcactc tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg
     5281 aatggatcaa agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct
     5341 atgctttttc gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac
     5401 agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga
     5461 aacaagaatt tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac
     5521 tccaatggtg tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca
     5581 gaacctttct agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt
     5641 tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt
     5701 ctagaactgc attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca
     5761 gtgttccatc attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg
     5821 atgatcaact gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca
     5881 gagaataatt gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc
     5941 accattccta aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga
     6001 tttactggta gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa
     6061 atgaaagcca ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct
     6121 tgatgcagcc ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt
     6181 gtaaaaatta ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc
     6241 acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc
     6301 ttttatttca taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa
     6361 caaaatcgtt cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc
     6421 aataaaatta gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc
     6481 aatcgtcaga cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc
     6541 ctgagcaaac cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg
     6601 tctcatgaca aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata
     6661 tacttgaaag cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc
     6721 taataaggtt gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga
     6781 aaactgaaaa tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg
     6841 tcccatgatg ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg
     6901 tttcatctgc tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt
     6961 cgcattcttt tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc
     7021 cagtctgatt tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt
     7081 tttcttttct tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc
     7141 tcgtagctga gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga
     7201 aaactctcgg atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa
     7261 atagcgatca tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag
     7321 tcgatcaata ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc
     7381 ataagaagaa agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg
     7441 ctctgaagtt tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt
     7501 tgcttcagcg agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt
     7561 cccatgatcg ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc
     7621 attttctcct atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag
     7681 ttctagctgg ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat
     7741 atgttttaca ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa
     7801 aatctgtttg caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac
     7861 taaatcacca aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat
     7921 actccatcca tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa
     7981 aagttcgaaa cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt
     8041 accacacacg attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg
     8101 aacatagtga tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt
     8161 cctattgata aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc
     8221 aactgatctg cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact
     8281 aacatgaatt gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct
     8341 cttgaaaaac tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt
     8401 ggcatcttcc ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc
     8461 atatcgccaa aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg
     8521 atcaaaggac gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt
     8581 ttcatattga cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg
     8641 tgtgggattt tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt
     8701 gaaaaatcat ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca
     8761 aagttgggta ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa
     8821 agccggggat agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt
     8881 ctgggctttt tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg
     8941 cgaaacatag ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat
     9001 ctgcaacttt tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat
     9061 gatctcgtat ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg
     9121 atcggctaat ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc
     9181 ctttctaata attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa
     9241 agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag
     9301 aaattttttg tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat
     9361 tattgattct tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc
     9421 aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta
     9481 tgcttaaaca gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac
     9541 ggtgttcaaa aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct
     9601 gtttttgttt ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta
     9661 ataaccataa aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc
     9721 cttagcactc aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata
     9781 aattggcttg gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt
     9841 taaagagttg atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta
     9901 taaacggtag acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca
     9961 gcctacagtt ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt
    10021 atgacggtta tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt
    10081 cagattatag gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata
    10141 acgacctgtt agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc
    10201 ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg
    10261 caatgattct ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc
    10321 attcacatat tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc
    10381 aaatagattg taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat
    10441 ggcttgagca atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt
    10501 tgaggcaata ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc
    10561 atagccagta tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg
    10621 cccatctgtc aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc
    10681 tgatgtactg tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga
    10741 aattgtattt tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc
    10801 tgtttctgaa gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac
    10861 tgttgtgttg gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc
    10921 tttcatcttt tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa
    10981 agtaaaagca agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct
    11041 ttttctgcat aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat
    11101 ggtcgtttga ataactattt aacaagtaat tctctctgga tattatccgt tatggattat
    11161 acttcctgtg tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct
    11221 atcgcaaatg ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct
    11281 tcatgaagtt caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca
    11341 gctcctggag agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct
    11401 tccatgattg cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca
    11461 acatgtcccg ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt
    11521 ggaagtacgg cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc
    11581 cattcatcac ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac
    11641 aatacatcga ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca
    11701 aaatctctta catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt
    11761 gcttttaggc gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat
    11821 agcttcgttg ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct
    11881 gataaatcat tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat
    11941 ctcttatttc atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg
    12001 attcctgcaa cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca
    12061 atggcattct cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca
    12121 tttttcttga tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga
    12181 tggttcaccg tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg
    12241 ccacctaagt caacgaacat cacttgacga ttgataaaga catttttccc tatactaata
    12301 tgtttgccga aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca
    12361 ctaatgatat tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc
    12421 gctgctaaag gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc
    12481 gttactcgaa tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct
    12541 ccttttatcc ttactttata acggacggct tatgatgtct aatacttata aaacatactt
    12601 aactattcct tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg
    12661 gatattcgta tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca
    12721 gcaaaatctc tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa
    12781 gaacttggtg tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt
    12841 cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat
    12901 ttaacaaatg acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca
    12961 atctctttca tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt
    13021 catttatata gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat
    13081 tttggactcg taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc
    13141 caagatcgtt ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc
    13201 catccgcagg aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt
    13261 caactagctg aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac
    13321 ttattgtaca atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat
    13381 gggatcatca atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa
    13441 gcttcactga atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt
    13501 ttagatgtcc taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt
    13561 ttaacaacga ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt
    13621 gttccttcct tttatttctg ataattccag taaattacta taattataaa aaaagaattg
    13681 acaaaacata actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact
    13741 agatcatact aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg
    13801 tctatttttg tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa
    13861 agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg
    13921 gtggacagga aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt
    13981 tgatttttga tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt
    14041 tgcaaaattt acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg
    14101 atcatgcgga aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg
    14161 accacattgc tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt
    14221 taagagaaat cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt
    14281 atctgacaaa agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc
    14341 atattcacag cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga
    14401 ttgtatctga catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac
    14461 cattttcact gaattattca agactggtga atcatttacg actgcttcta caacgaaccc
    14521 atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc
    14581 cagaaagcta taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg
    14641 cgatcacgaa agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa
    14701 tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg
    14761 aatgggacgt tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg
    14821 gatcggaaac tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg
    14881 cggtaacgca gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat
    14941 gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt
    15001 aaatgtttta aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga
    15061 tccatctttt gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc
    15121 agctctttat aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg
    15181 caagcgtcta gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact
    15241 gctcgtatgg cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt
    15301 aggctggggt ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt
    15361 gcatcaagca atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa
    15421 atttttggct tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc
    15481 aatcatgatg ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga
    15541 aaacaaagca aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg
    15601 tgtaacagaa ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca
    15661 tgcaattttc actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt
    15721 tgcctttagt gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca
    15781 gccattgatg ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa
    15841 ctttgctatc aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga
    15901 tgacgatact gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta
    15961 cgctgcatta ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg
    16021 tttgcaagtc aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc
    16081 tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt
    16141 cgttgcagat gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa
    16201 caccgtggtt aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat
    16261 cgcaaatggg aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat
    16321 gatgggtgac ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg
    16381 tacgatcaca aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat
    16441 cgaagtcttg cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt
    16501 atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc
    16561 cgctattcaa tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa
    16621 agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc
    16681 tgtttccaac aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag
    16741 gagctgagac aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc
    16801 caactccgcg gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac
    16861 tcttcttatt acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa
    16921 accagtttat cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa
    16981 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    17041 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    17101 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    17161 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    17221 cttgcttcaa taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct
    17281 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    17341 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    17401 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    17461 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    17521 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    17581 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    17641 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    17701 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    17761 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    17821 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    17881 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    17941 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    18001 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    18061 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    18121 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    18181 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    18241 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    18301 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    18361 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    18421 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    18481 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    18541 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    18601 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    18661 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    18721 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    18781 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    18841 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    18901 gattctgcta aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta
    18961 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    19021 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    19081 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    19141 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    19201 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    19261 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    19321 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    19381 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    19441 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    19501 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    19561 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca
    19621 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    19681 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    19741 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    19801 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    19861 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    19921 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    19981 ccatctagga gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    20041 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    20101 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    20161 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    20221 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    20281 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    20341 tctatgtttt acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc
    20401 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    20461 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    20521 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    20581 ataaaa
//
LOCUS       DARBEG010000045        20484 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000045 DARBEG010000000
VERSION     DARBEG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.045"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="SCK20_001650"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="SCK20_001650"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG1145705.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="SCK20_001651"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="SCK20_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG1145706.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="SCK20_001652"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="SCK20_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG1145707.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="SCK20_001653"
     CDS             2856..3731
                     /locus_tag="SCK20_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1145708.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="SCK20_001654"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="SCK20_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG1145709.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="SCK20_001655"
     CDS             4628..5062
                     /locus_tag="SCK20_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG1145710.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="SCK20_001656"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="SCK20_001656"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG1145711.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="SCK20_001657"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="SCK20_001657"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG1145712.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="SCK20_001658"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="SCK20_001658"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG1145713.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="SCK20_001659"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="SCK20_001659"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG1145714.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="SCK20_001660"
     CDS             13333..14259
                     /locus_tag="SCK20_001660"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG1145715.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="SCK20_001661"
     CDS             14441..15334
                     /locus_tag="SCK20_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG1145716.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="SCK20_001662"
     CDS             15468..15593
                     /locus_tag="SCK20_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145717.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="SCK20_001663"
     CDS             complement(15727..16359)
                     /locus_tag="SCK20_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145718.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="SCK20_001664"
     CDS             complement(16507..17049)
                     /locus_tag="SCK20_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG1145719.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="SCK20_001665"
     CDS             17184..17573
                     /locus_tag="SCK20_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145720.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="SCK20_001666"
     CDS             17577..18341
                     /locus_tag="SCK20_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1145721.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="SCK20_001667"
     CDS             18470..19417
                     /locus_tag="SCK20_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1145722.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="SCK20_001668"
     CDS             19547..20287
                     /locus_tag="SCK20_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145723.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7192 a         3481 c         4316 g         5495 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agtt
//
LOCUS       DARBEG010000046        19821 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000046 DARBEG010000000
VERSION     DARBEG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.046"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SCK20_001669"
     CDS             6..305
                     /locus_tag="SCK20_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1145724.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SCK20_001670"
     CDS             378..1739
                     /locus_tag="SCK20_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG1145725.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SCK20_001671"
     CDS             1761..2021
                     /locus_tag="SCK20_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145726.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SCK20_001672"
     CDS             2035..2787
                     /locus_tag="SCK20_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG1145727.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SCK20_001673"
     CDS             complement(2881..3486)
                     /locus_tag="SCK20_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1145728.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SCK20_001674"
     CDS             complement(3539..4513)
                     /locus_tag="SCK20_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG1145729.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SCK20_001675"
     CDS             complement(4891..5610)
                     /locus_tag="SCK20_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1145730.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCK20_001676"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCK20_001676"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1145731.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SCK20_001677"
     CDS             7161..8558
                     /locus_tag="SCK20_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1145732.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SCK20_001678"
     CDS             8639..9079
                     /locus_tag="SCK20_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1145733.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SCK20_001679"
     CDS             9227..9553
                     /locus_tag="SCK20_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1145734.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="SCK20_001680"
     CDS             9554..10303
                     /locus_tag="SCK20_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145735.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="SCK20_001681"
     CDS             complement(10383..10838)
                     /locus_tag="SCK20_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1145736.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="SCK20_001682"
     CDS             complement(11265..11540)
                     /locus_tag="SCK20_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145737.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="SCK20_001683"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="SCK20_001683"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG1145738.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="SCK20_001684"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="SCK20_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1145739.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="SCK20_001685"
     CDS             14770..17436
                     /locus_tag="SCK20_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1145740.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="SCK20_001686"
     CDS             17800..18552
                     /locus_tag="SCK20_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1145741.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="SCK20_001687"
     CDS             complement(18574..19728)
                     /locus_tag="SCK20_001687"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG1145742.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6329 a         3472 c         4060 g         5960 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DARBEG010000047        19778 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000047 DARBEG010000000
VERSION     DARBEG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.047"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SCK20_001688"
     CDS             99..293
                     /locus_tag="SCK20_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145743.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="SCK20_001689"
     CDS             562..861
                     /locus_tag="SCK20_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145744.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="SCK20_001690"
     CDS             complement(958..1392)
                     /locus_tag="SCK20_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145745.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="SCK20_001691"
     CDS             complement(1624..2334)
                     /locus_tag="SCK20_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1145746.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="SCK20_001692"
     CDS             complement(2324..3184)
                     /locus_tag="SCK20_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG1145747.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="SCK20_001693"
     CDS             complement(3189..3827)
                     /locus_tag="SCK20_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG1145748.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="SCK20_001694"
     CDS             complement(3842..4141)
                     /locus_tag="SCK20_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1145749.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="SCK20_001695"
     CDS             complement(4184..5668)
                     /locus_tag="SCK20_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1145750.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="SCK20_001696"
     CDS             complement(5688..6149)
                     /locus_tag="SCK20_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1145751.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCK20_001697"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCK20_001697"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG1145752.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="SCK20_001698"
     CDS             7452..8222
                     /locus_tag="SCK20_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1145753.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="SCK20_001699"
     CDS             complement(8495..9322)
                     /locus_tag="SCK20_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1145754.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="SCK20_001700"
     CDS             9890..11125
                     /locus_tag="SCK20_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1145755.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="SCK20_001701"
     CDS             complement(12124..12384)
                     /locus_tag="SCK20_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1145756.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="SCK20_001702"
     CDS             complement(12411..13712)
                     /locus_tag="SCK20_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1145757.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="SCK20_001703"
     CDS             complement(14004..14462)
                     /locus_tag="SCK20_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1145758.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="SCK20_001704"
     CDS             complement(14475..15806)
                     /locus_tag="SCK20_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG1145759.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="SCK20_001705"
     CDS             complement(15817..17259)
                     /locus_tag="SCK20_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1145760.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="SCK20_001706"
     CDS             complement(17246..18010)
                     /locus_tag="SCK20_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG1145761.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="SCK20_001707"
     CDS             complement(18114..18989)
                     /locus_tag="SCK20_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1145762.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="SCK20_001708"
     tRNA            complement(19244..19328)
                     /locus_tag="SCK20_001708"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="SCK20_001709"
     tRNA            complement(19346..19417)
                     /locus_tag="SCK20_001709"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="SCK20_001710"
     tRNA            complement(19427..19499)
                     /locus_tag="SCK20_001710"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="SCK20_001711"
     tRNA            complement(19561..19645)
                     /locus_tag="SCK20_001711"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="SCK20_001712"
     tRNA            complement(19666..19736)
                     /locus_tag="SCK20_001712"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
BASE COUNT         6276 a         4090 c         3077 g         6335 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatag
//
LOCUS       DARBEG010000048        19331 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000048 DARBEG010000000
VERSION     DARBEG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.048"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            233..2704
                     /locus_tag="SCK20_001713"
     CDS             233..2704
                     /locus_tag="SCK20_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1145763.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2807..4543)
                     /locus_tag="SCK20_001714"
     CDS             complement(2807..4543)
                     /locus_tag="SCK20_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG1145764.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4540..5244)
                     /locus_tag="SCK20_001715"
     CDS             complement(4540..5244)
                     /locus_tag="SCK20_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1145765.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5375..5878
                     /locus_tag="SCK20_001716"
     CDS             5375..5878
                     /locus_tag="SCK20_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG1145766.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5838..6176
                     /locus_tag="SCK20_001717"
     CDS             5838..6176
                     /locus_tag="SCK20_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG1145767.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6197..7615
                     /gene="ffh"
                     /locus_tag="SCK20_001718"
     CDS             6197..7615
                     /gene="ffh"
                     /locus_tag="SCK20_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG1145768.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7727..8305
                     /locus_tag="SCK20_001719"
     CDS             7727..8305
                     /locus_tag="SCK20_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG1145769.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8309..8830
                     /locus_tag="SCK20_001720"
     CDS             8309..8830
                     /locus_tag="SCK20_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1145770.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8909..9463)
                     /locus_tag="SCK20_001721"
     CDS             complement(8909..9463)
                     /locus_tag="SCK20_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1145771.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9716..9991
                     /gene="rpsP"
                     /locus_tag="SCK20_001722"
     CDS             9716..9991
                     /gene="rpsP"
                     /locus_tag="SCK20_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG1145772.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10003..10254
                     /locus_tag="SCK20_001723"
     CDS             10003..10254
                     /locus_tag="SCK20_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG1145773.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10379..11170
                     /locus_tag="SCK20_001724"
     CDS             10379..11170
                     /locus_tag="SCK20_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145774.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11340..11861
                     /gene="rimM"
                     /locus_tag="SCK20_001725"
     CDS             11340..11861
                     /gene="rimM"
                     /locus_tag="SCK20_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG1145775.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11851..12612
                     /gene="trmD"
                     /locus_tag="SCK20_001726"
     CDS             11851..12612
                     /gene="trmD"
                     /locus_tag="SCK20_001726"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG1145776.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12748..13095
                     /gene="rplS"
                     /locus_tag="SCK20_001727"
     CDS             12748..13095
                     /gene="rplS"
                     /locus_tag="SCK20_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG1145777.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13227..14363)
                     /locus_tag="SCK20_001728"
     CDS             complement(13227..14363)
                     /locus_tag="SCK20_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1145778.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14479..15393)
                     /locus_tag="SCK20_001729"
     CDS             complement(14479..15393)
                     /locus_tag="SCK20_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1145779.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15484..15912)
                     /locus_tag="SCK20_001730"
     CDS             complement(15484..15912)
                     /locus_tag="SCK20_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1145780.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15917..16291)
                     /locus_tag="SCK20_001731"
     CDS             complement(15917..16291)
                     /locus_tag="SCK20_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1145781.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            16577..16762
                     /locus_tag="SCK20_001732"
     CDS             16577..16762
                     /locus_tag="SCK20_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1145782.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            16774..17022
                     /locus_tag="SCK20_001733"
     CDS             16774..17022
                     /locus_tag="SCK20_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145783.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17053..17232
                     /locus_tag="SCK20_001734"
     CDS             17053..17232
                     /locus_tag="SCK20_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145784.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17275..17745)
                     /locus_tag="SCK20_001735"
     CDS             complement(17275..17745)
                     /locus_tag="SCK20_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145785.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            17832..18530
                     /locus_tag="SCK20_001736"
     CDS             17832..18530
                     /locus_tag="SCK20_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1145786.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            18708..19049
                     /locus_tag="SCK20_001737"
     CDS             18708..19049
                     /locus_tag="SCK20_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145787.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19042..>19331
                     /locus_tag="SCK20_001738"
     CDS             19042..>19331
                     /locus_tag="SCK20_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1145788.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         6368 a         3377 c         3804 g         5782 t
ORIGIN      
        1 attttaccaa aagtgagtct ctttttgagt tgaaacgaag aaaaagtgtt agtagaaaat
       61 cgccttcgat tttccaccaa cacaaaatat tatagaaccc tttttttttg ctatatgaca
      121 ggaatgaaag gtgtttgcaa gcttaatgtg aggaacactt tccatatctg ttctatactg
      181 gataggagaa gtttggaaat atccagaaaa cgaaatcaat cgaggtagcc acatgaagaa
      241 taaatggata aatcgaagta aaaaaaagaa agtaacacaa agatcatcag ataaagtcat
      301 ccgtcttttt ccatcgtata aagaaggatt aacggcagat caagtcaagg aacgaataga
      361 aaaaggtgca gcaaataatt cagtcgatcc cacattcaaa acaaatcagc aaatcgtatt
      421 ggaaaacata tttacgtatt tcaatttgat ctttcttatc ttagccattt tgctttgttt
      481 agtagaatcc tataagaact tgactttttt accagttatc atagccaata caggaatcgc
      541 catttttcag gaaattcgat ccaaaaagat cttggacgaa ctaaatgttt taaatacagc
      601 aaaagtcaat gtcatacgag acgggataga acaaacaata gatatagaag accttgtaat
      661 agatgacatt gttattttag agactggata tcaaattcca gcagatgcag aggtcgtgga
      721 agggaaactg caagttaatg aggctttatt gacaggcgaa gcagatgaaa taaccaaaga
      781 aataggtgat ccattaatgt ccggcagttt cattgtttcc ggaaaagctt acgcccgatt
      841 agacaaagtt ggagcagatt cgtatatttc ccagctgaca gtcaaagcca agacaatggg
      901 agaaggagaa cagtcggaga tgattgcttc tcttaatcaa ttgatcaaat ggattgggat
      961 catcattatt cctatcggta tcctgctttt ttctcaaagt tactttttta atggaaacac
     1021 gatcaaagaa agtgtcgttg caatggaagc agcattgatc ggtatgatac ctgaaggact
     1081 gtacttgctg acgacgattg cattagcatt aagtgcgact aaactagcaa agcaaagagt
     1141 tcttttacac aatatgaaaa gcattgagac attggcaagg gtaaatgtcc tttgtgtaga
     1201 taaaacagga acgatcacag aaaataaaat gtctgttcaa aaaatgatcg tctctaaaaa
     1261 acaagaaata atcagtcagt cagatcaatt ggaagcatgc attgctgact atgcaaaagc
     1321 gatggcagga gacaatgcaa caatggaagc tgttaaagca tattttacaa aaaccactga
     1381 tcaatcctat caccatgtta ttcctttttc ttctgtccaa aaattcagca gtgtagcgtt
     1441 ggcagacaag gtgtatgttt taggagcgcc cgaaatggtt ttgcgcgatc aattgccgga
     1501 atatgcggaa gagtttgttt ctttcgctga aaaaggctat cgcgtgttgg tctttggtgt
     1561 gtacgatggg attcttgaag aaccagtatt gacagaagca gtcacaccat taggctatat
     1621 tctgattgct aatcctatca gaaaagaagc aagagccaca tttgattact tcaaaaaaca
     1681 gcaagtagcc atcaaagtga tttctggaga taatccgctc accgtatcca atgtagctgt
     1741 ccaagcaggg atttcacatg ctgatcagta cgttgatgca tctaaattat cagaagaaga
     1801 atatgaagca gctgtcgaaa aatatacagt ttttggacgc gtcaagccag aacaaaagaa
     1861 aatcttcgtt caattgataa agcgtaacaa tacagtagct atgacaggtg atggcgtgaa
     1921 tgatattcta gccatgaaag aagcagactg cagtatagcg atggcttccg gaaatgaagc
     1981 cgccatgcag gcctcacaag tggtcttact tgattctgac ttttcacgca tgccagaagt
     2041 tgttgcagaa ggacggcgcg ttgtgaacaa tattgagcga tcagccagct tgtttttagt
     2101 gaagaatatt ttctcctttt tgctctcact gttttcagtg atctttgctc tgacctatcc
     2161 tttagaacca tcacaaatca cattgattag tttgtttact atcggacttc catctttctt
     2221 gcttgctcta gaagaaaaca aaaagcgaat cagagggaaa ttcatcatga atgtgatgga
     2281 aaaagctgtg ccaggtggac taacagatat gatcgtagtc ggtgcgttgg tgatctgcgg
     2341 ggtgactttg aatctaaata aaacagatgt ttctacagca tctaccatgc ttttgattgc
     2401 agtagggttc ctcgttctct ataaaatctg ttcaccgctg aataaattca gaagtcagat
     2461 cattcttttc tgtgctagcg gtaacttttt ttcggtcatc tttttgcata agctgttttc
     2521 aatcaccggt atctcagctg tatcactttt gctgttgagc attctatttt tctcggcaga
     2581 ttcgattttt aggcatttga ccacattggt agaatatttg tttgaacgta aaaatgaaaa
     2641 agagcaaaag ccgtttagtc gcgtagctaa aaaatttcta tcgatattta aacgagaaaa
     2701 ttgacgaaaa gtatctcaaa aaagagtaaa aaagaacaac cactcgtgta tctttcgaag
     2761 caggatgagt catccaactt taaaacgcac gagcagttgc tcttttttac attttcggaa
     2821 tggtaacggt gaaagtcgaa cctgttccaa gatgactgtc tacagtaatc gtaccattaa
     2881 ggagttgcac gtagtctttt acaatcgcta accctaatcc ggtcccgcct gaatgtcggc
     2941 ttcgcgcctt gtctacgcgg taaaaacgct caaagatccg ttcctgatcc tcaatgtcga
     3001 tacctatacc aaagtcttgg acagaaaacg atagatcatc tgttgcctga tagcggataa
     3061 taatctcgct gtcgcttttt gaatattgga cagcattttc aattagattt ttgataattg
     3121 gataaaggat atctgtttta gtagtaaatg gttcgttctt tccaatcaat cgaatcgtca
     3181 accgtttctt ttcgatgatt ggctgataat tttgaacgat ttgctggaaa taaggttctg
     3241 tataaattgt ctggatctcg taagaattct cgccatcttt tgacagctgg atgatttccc
     3301 gaatcagttt atctaaccgg atcgcatctt tttgcatgat ctcaagaaaa gaagtcagag
     3361 tttgcgggtc atcttttgca ccatccagta aagtctcggt aaaaccaatc agtgaagtca
     3421 caggagtttt taattcgtga gagacgttgc caacgaaatc cttttgtagc ttttctaaac
     3481 gtcgcacttt cgttaagtca tacgccaccc ctagaatctg tccggtacct tctgaattag
     3541 taaagtagcg caaactcaag tctaatgtct gccgaccttc tgtaagggtg atctcctgat
     3601 ggatcagcgg atgctcagga gtcacttgat gaatcagctg aatgagtttt ggctcttgaa
     3661 tgacttctgt gtaccgctgt tcaggttgat agcttaccac gtttaaatgt agctgcatct
     3721 ttggattaag cagtagtaaa cgagaatcag catcatcaat caggaatacg ccaatcatca
     3781 gttcgttcag taacgtatat aattgctctt ctgtggtagt acacgcccga tacatcttac
     3841 tcatttgttc gctcaacgca ttaatcgtct gatacaattc ttcccattga tcgggtgttt
     3901 gcatgattac ttccgcctga ttaggatttt gcaccatttt ttttagtacg ggcagcacgg
     3961 ttttcaatgg ctggtttttt tgatagatca gataatagat cattcctgca agaacaagga
     4021 agaaagacag aaagaagaaa aagacccaac ggcgaaaact ctcagttctc ggtaaaaaac
     4081 cgcttgtcgg ttctgcgata cgtaaaatgg cttctaactg gccacttttt ttgactggta
     4141 atgcgacata taaaagatcc tcattcaaag tagtactttt gcgcaaagcg ctacctaggt
     4201 tcccaccatt caatacggct ttgatttcgg gacgggagtt tcgctgttca ttcaatgttt
     4261 cattgtttgt gtcaaataag atctttccag tagcatccat taaagtaatc cgttcgttcg
     4321 actgatggac gaattcttcc aaagcagttt tgttttgcgc tgcttggagg ttttcgacat
     4381 ctaattgccg gatcagcagt tgtgcttttt ttgtcaggta gctttcttgc tgttccacga
     4441 tctgctgttg aaaataatgg gaaatcaatt gccagccacc aaaaaacaaa ctaactaaaa
     4501 gaataagaaa gaagagacgt tcataaattt ttttccattt catcgtttgg gttcctgaaa
     4561 cttatagcca aatcctctaa tagtcaccaa gtactttggg tgttttggat ctcgctcgat
     4621 tttttctcgc aaatgactaa cgtgtacatc tacgattcgg ctttgtcctg cgaagtcaaa
     4681 attccaaatg cggtctagca atgtgtctcg gtcgatcaca cggtcttttc gtttcatgaa
     4741 ataaacaagt aattcaaatt ctttcggtgt tagttcaatc agatggtcgt ctacggtcac
     4801 ttgataattg gtcaaatcag cagtgatttg tccaatcgat agatattcag gctcagcttc
     4861 atcttttgat tgcttacgag gttcaattcg tctgaatatt gctttcatac gggctaatac
     4921 ttcgcgagga ctaaatggtt tggtcaaata gtcgtcagca cctatttcta ggccaatgat
     4981 ccgatccact tggtcatctt tagccgtcag catcaaaatc ggtgtttcat ttttttcttg
     5041 acgcagccgc tgagtgatct ccatgccatc catcgttgga agcatcacat ctaaaatgat
     5101 gaaatcaaat gattgttcta atgctaattc taatccttcg cctccatctg tggcactggt
     5161 taccttgtag ccttcttttt ccaaattgaa cgtcaagagc gttacaatag aaggttcatc
     5221 atcaacaact agtatttttt tcattcaaat ctcctttaaa taggtttcat tctgtttata
     5281 ttttagcata gaatcagtac aaagggctat tttacaaaag aaataaatgg tataatagga
     5341 aggcactttg ctgaatagaa aggaagagca gtcaatgatt ggaatcagtg catgtttagg
     5401 cggtctggcc tgtcgctatg atggacagaa aaaagaagta ccagaattga tggaactagt
     5461 tgaaactgga gaagctgtca tggtctgtcc agaagtgatc ggcggattac caattcctag
     5521 agatcccgct gaaatcattg gtggaaatgg attcgatgtg tggaaagatc aagcaaaagt
     5581 atggacgaca agcggcgaag atgtaacgga agcgtacaaa aatggcgcga tcaaagccta
     5641 tcaaaaattg caggaaaaac acattgatct agtcatcatg aaggaaaaaa gcccttcatg
     5701 cggaacccat tcgatttatg acggaagttt ttccggaata aaaaaaaatg gtgccggagt
     5761 ggctactgct tattttattc agcaaaaaat gactgtgctc tctgatgaag aatggctgaa
     5821 aactagaggt gagcagaatg gaaatcgaaa agaccaatcg aatgaacgcg ctctttgaat
     5881 tttattcgac cttattgaca gaaaaacaaa tgaactacat ggaattatat tatgcagatg
     5941 atttttcatt aggtgagatc gcggaagaat acgaagtaag ccgacaagca gtatatgaca
     6001 atatcaagcg gacaagcaag atcttggagg attatgaaaa aaaactccat cttttttcta
     6061 attatattgt tcgagaacag gtattggaaa atatgactgc atatattaca gataagtacc
     6121 cggaagataa aaaactactt gaatatgtac agcaaattca agcaatcgaa gaataaataa
     6181 aaggaatgaa gaaaatatgg catttgaaag cttaactgaa cgtctccagc aggcgatggg
     6241 aaaaataaga aaaaaaggaa aagtatcaga agctgacgta aaagaaatga tgcgggaaat
     6301 ccgccttgcc ttactagaag ccgatgttaa cttgcaagta gtcaaagatt ttactaagcg
     6361 cgtacgcgaa cgtgcggtag gggcagaagt gctggaaagt ttatcacctg cacaacaaat
     6421 cgtaaaaatc gttgatgaag aattgacgat cacacttggt tctgaaacag cagagttaaa
     6481 taaatcacca aaaataccaa ctgttatcat gatggctggt cttcaagggg caggtaaaac
     6541 gacctttact ggtaaattag ccaattattt aaagaaaaat gaaaatgctc gtccgctcct
     6601 aatcgcaggg gacgtatatc gaccagctgc gatcgaccaa ttgaaagtat taggtcaaca
     6661 gctagacgtt cctgtatttg atatgggaac tgaagtcagc ccggtagaga tcgtccgtca
     6721 agggatggag ttagcaaaag aaaagaaaaa cgactatgta ttgatcgata cggcaggacg
     6781 tctgcatatc gatgaaacat tgatggatga gttgaaacaa atcaaagaat tgacgcaacc
     6841 aaatgagatt ttgcttgttg tcgatgccat gactggtcaa gatgccgtca acgtagctga
     6901 tagcttcaac cagcaattag gtattacagg ggtcgtcatc actaaacttg atggggatac
     6961 tcgaggcggg gcagcactat cgatccgctc agtgacaggc gcaccaatca aattcatcgg
     7021 ttctggtgaa aaattgactg acttagaagt attccaccca gatcgtatgg ctagccgtat
     7081 ccttggtatg ggggatatgc tgacattgat tgaaaaagcg cagcaagact acgacgagaa
     7141 aaaagcggaa gaacttgcac agaaaatgcg ggaaaattca ttcgatttca atgactttat
     7201 tgaacagctt gatcaagtga tgggtatggg cccactggaa gatctaatca aaatgatccc
     7261 aggaatgaat caagtccctg gaatcgaaaa cgtcaaggta gatccaaaag atgttgaacg
     7321 caaaaaagcg atggtttatt ctatgacgcc agcagaaaga gcaaatcctg atctgttgaa
     7381 cccaagccgt cgtcgccgaa ttgctgccgg ttctggaaac agtgttgttg aagtgaatcg
     7441 aatgatcaaa caattcaagg aatcaagaaa aatgatgcag caaatgtcca aaggggatat
     7501 gaacatccca ggtatggacc aaatgtttgg ttcaggcgtc aaaggaaaac taggcaaaat
     7561 ggcgatgaat cgaatgatca agaaaaataa gaagaaaaag aaaaaacgga aataagatga
     7621 aaaaaacagc ttcggtacag actgaaaatg tctactgccg gagcttttct atcagcaatt
     7681 tttattcttt actaatacaa ttactctaaa cgaagaggtg gcacagatga gatgtccgtg
     7741 gagtgaacaa acagaaagta tgcggcaata tcatgatact gtctggggtg ttcctgaata
     7801 tgatgaccaa aagctgttcc gaaaattgat gcttgatatt aatcaagccg gattgagctg
     7861 gcagacgatt ttaaataaaa gtgaagcatt cgatgaagca tatgatcgat tcgatatcaa
     7921 aaaagtcgca aattatggag aaacaaaaat cagtgaacta atggaaaaca aaggaattat
     7981 tcgtaaccga agaaaaatcg aagcagccgt ccaaaacgcc aaagcagtct tgaaaattca
     8041 agaagaaatc ggcagttttt cagagtatct ttggtcgttt tctaatggaa aaatcgttga
     8101 ttcttcttat cattcacaag aagaagtccc aacaacaagt cctctttcag atgaattagc
     8161 aaaagatcta aagaaaagag gatttaaatt tatcggaagc acaacaatct atgctttttt
     8221 agaagctgtc gggatcatca atgatcacct tgacagttgt ttccgcaaag aagaggtcgt
     8281 acgtatcggc agaaagggcg aataaagcat gcgaaaaatc atattttatg gtgctataag
     8341 tttagatggc tatttggcaa cgaaaacgga tgatcttcaa tggctttttg acactccaac
     8401 aggtaagcaa actacttatg aagcttttta tgaaacaatc gatacgacca tcatgggcag
     8461 aaaaacttat caggaagcaa aaaaatattt ggagaccgaa gcaatttatc cagagaaaaa
     8521 gaatttcgtt ttttccaccc acgcacaatt acaattgcca gatgctgagg tcgtaaacga
     8581 agatcctgtt gcttttctaa aaaatctaca aatgacagaa ggaaaagata tctgggttgt
     8641 gggtggcggt cgattgatca aaccgctatt ggaacatcaa atgattgatg aatggtatat
     8701 ccagattacc cctgtattat taggggacgg gattccttta ttccaaaaag gaacatatga
     8761 agcgcgtttt gaaatgctag acacgacacg attcggtgaa tttatcgaat tgcattatgt
     8821 acgaaagtag aagtaaataa aataaaaaag gcaactatat gcagaaatgt cctgtacata
     8881 gttgcctttt ttattaccgt tatgcttttt actctgttaa tttgttagaa aaatctagat
     8941 acgcatctaa aatcgtttgg aagaaactta atgttccttc aacgatatta ccattttcat
     9001 caattagatt cgcaacgttt ccgatatatg cttccggttg ctgcaaggtt ggcacattca
     9061 aaaagacaag tgattggcgt agatggtggt ttgcgccaaa gccgccgatc ccacctggtg
     9121 atgcagtaac gactaaacct ggtttatgat cccaaacact gtgtccataa ggtcttgagc
     9181 ctacatctaa agcatttttc aatactgctg gcacagaacg gttgtattca ggagtaacga
     9241 aaacgacacc gtctagtccc ctcacttcct cacgaaaacg agtccattca gcaggagcct
     9301 gttctggagt ttcgagatcc tcattgtaaa atggcagatc atcgattttt acaaatacac
     9361 tttcgtaccc ttcaggaagc aatttcgcaa aagcttctgc tacttttttg ttgtaagaat
     9421 cttttcttaa actaccaata aaaaatccaa ttttttttgt catacgattt ttcctccttc
     9481 aaactatcta taataatctt acaaaagata agtaactgga gcaaataaaa acacttactg
     9541 acaaaagcta catagaagat agattactta tttttaacaa aaaagaagtc taaaatcttc
     9601 tattttctaa aaaaaatcga atttaatcaa gctgtaaaga aaaaaagctt tacagggtaa
     9661 aaaaaaactg ttacactaca tcttgtgaat aaaacaatgg aggtggaaat aaataatggc
     9721 agttaaaatc cgtttaaaac gtatgggttc taaaaagagt ccgttttacc gtatcgtcgt
     9781 agctgattca cgttctcctc gtgatggacg tttcatcgaa actgtaggta cttacaatcc
     9841 tttgaaagac cctgcagaag tagttttaaa agaagactta gttcttgact ggttatcaaa
     9901 aggtgcacaa ccttctgaca ctgtacgtaa catcctttca aaagaaggcg ttatgcaaaa
     9961 acaccacgaa gctaaattct caaagaaata aggtgaccgg atatgaaaga tttaagcgac
    10021 ttagtcttaa caatcgttcg tccgctagtc acttatcctg atcaagtcaa gttagacgtc
    10081 gtagagtcga aagactttta cgagtacaat ctaacagtgg ctcctgaaga tattggccgt
    10141 atcattggaa aacaagggcg tgttgctaaa gcaatccgca caatcgttta cggtgttcgg
    10201 atcaatgcac cgaaaaaagt gcgtttgaat atcatcgatg ataaggaaaa gtaaataaac
    10261 gtgctgtact cgaattttcg agtgcagctt ttttgttttt tttattaatt gaagaaatga
    10321 ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg aacagccaat
    10381 gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta gttcggttac
    10441 tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta ccagtccaga
    10501 aaaaagtagt accaaaacgg cttccaccac tgaacaaaca atggctacct caaataatga
    10561 gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca tgcctctaga
    10621 tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc aaggttattc
    10681 tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat tgaatcaaga
    10741 aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag aaacaatcca
    10801 agttttgcag ccatttgaga tcgacacaaa tggtcagcaa gtggacttag gttctcgaat
    10861 cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag aaggaaactg
    10921 gcgtattcga atccgtggga ataatataga aggccaagat cccttactat tagctaaaga
    10981 aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg gtaaaattac
    11041 tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag aaccgaaaaa
    11101 tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag tttctatgaa
    11161 gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt cacaggaagt
    11221 aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta aaagaaaact
    11281 agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg ggatataagt
    11341 tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa ggcgaagtcc
    11401 gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg gtgctaacac
    11461 tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc aaacataaaa
    11521 attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa aaatatcggg
    11581 atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat gaattctatt
    11641 accatgagat catcggtctg catgtattag aagatggaac tgagttaggt aaagtaaaag
    11701 aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa aaagctgata
    11761 tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct ggagtggtcc
    11821 gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac tttatttcca
    11881 agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga taaaggatta
    11941 ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca gacagtagat
    12001 gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat ctatgataac
    12061 atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct tcttgaccca
    12121 gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga acacttagta
    12181 ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt gacagatgaa
    12241 gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt catgatcgat
    12301 gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca aacggattct
    12361 tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata taaagggatg
    12421 aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg gcaattaaaa
    12481 gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact tgaatggaca
    12541 cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa aacagcagat
    12601 gaagccaagt gactttttct aaaatcttct ttacagtagg ttgataatat gttagtattt
    12661 tatggtgagt gcaaaagcac ttaactatta cgatattccg ctgtgagaaa agctcataag
    12721 aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt aacaaaagaa
    12781 caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt tcacgcaaaa
    12841 gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat caaacgccgc
    12901 ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt gggcgttgaa
    12961 cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg ctacggtaaa
    13021 gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc tcgtatcaaa
    13081 gaaatccgtc gttaatcatc gttatcagac tgagaaagaa cccttggtat tcaagggttc
    13141 tttttttgtt ctctgttttt ttatattaac gatttctatt tgggacattt ttgattaata
    13201 aaaaggggca aaaaaggggc aacaagttac agtttttcta tatcttcttt caatttttct
    13261 tttactccct ttgtcacatg caaataaatc ttttcagtga tatcactatt ttcgtgccca
    13321 acgcgatcct gaatagcata cagaggagtt cctagttccg ctaattttga aatatgggta
    13381 tgtctaaaaa tatgtgaact aagtttttta tcaatcttca tgtcagcttt atgatttctc
    13441 aaataggtgt taattgctgt tagttggaaa ggagttcctt tcgttgtttg aaatagaaat
    13501 tgaccattcg gatttaattc tagaagctca ttgtaaatag ctatcgcttt ttttggtaaa
    13561 tcaatttctc gcattccagc agcagttttt gtagaatcag attttttcat atcagctatt
    13621 gaacgttctc tatacatcat cgtcccattt ataactactg atgcattatt attggtaatg
    13681 tgtacatcgt ctttacttaa tgcgattgct tctccaggcc tcattccagt caaatatagc
    13741 cactggaaaa ggagagaata tcttttattg tgtgaagttg tgaaatttac tagtctttta
    13801 tattcatcat cttctaaaaa cttatcttta atttttatcg tttttgactc tcgtttataa
    13861 tcaataacta cttcgttgat aggatttttt tctacatagc cttttttcat agcgtatgaa
    13921 aaaagaagat tcaatttgga tttaattaca ctgacgtact tatttgacaa gtcatcttta
    13981 tatatcatat cttcaaaaat attatttaaa tctactgtat taataccaga aacgatgtaa
    14041 gtctcaggta tctttttctt tatagtgttt aagatattat tcgtaggata atatgtagac
    14101 tccttaactt gtcttttgta aataactaac cattcttcga caagctcatg aaatgttaga
    14161 tcaggcttct gaagtgtttt ttcgtttaat tttatatcaa tttttttatt taactctagc
    14221 aatgccactt tctgagtttc tcgagattta tttttataag taatgctcac tttttttctt
    14281 tttctagttt ttggatcaat atatcgctca ttatatttat atactttttt tccgttttta
    14341 tcagttttgg tttcaatcca cattgttttc atctcctatt tgttgctata ataggaatgg
    14401 ataagtaagc caattatagc aggtttattt ttcatcacgt ccacaaactt tggcgaggga
    14461 gggggcgtgt ttttttgttt agtagctacc tttccatgaa gtatctgcga agaacacttc
    14521 aaatgttgtt ccatcatttt tcacttcaaa atatgctgtt ccttgtgcgg atttacccgg
    14581 ttggatggtt tccgaaaagt aatctctact agctaaatca gatttaatat ctgatgaatc
    14641 ataaaattct aaataacttg aacttgcatc aaaaggtgta gtccctgtgt tttctacaag
    14701 aaaatctacc tttgcgtaaa ataggccttc tggtttatag aaatcatcac cgtaatcttt
    14761 tgatacagac tggacagtta cgttaataga gttaccattt tcagaagtaa aaggaactgc
    14821 ttctcctata ctatgtgcaa ctgatgccgt ctggggttct gaagacgaag ttgtagttgt
    14881 agttgtagta gagcttgaat ctttggattt tccatcttta tctaagaaag ttcgttgatt
    14941 ataatataca acctgttcag tttttggact aacccaaatt aacattgttt catcatcaga
    15001 atcagttgaa taagtatatt ggagcatctc taattttttt atatcttttg cactatttcc
    15061 atgttcaatt aattcagagt gctctttata ataatctcct tgtgcatcct ttgaaataga
    15121 tacaattaat tcatcccgtg tgatagcatt gtctaattct ttgtaaacaa aatcatttgt
    15181 ccatttacgt aacggtgaac caagtgtctg ataaacttga gttaaatcat caccaacttt
    15241 tattttatga acgtctgatg aagttagttt ttttgtttca tattcagatg ttgatgaaac
    15301 agtattttct ttagtgctag cagaattagg actagtggag catccgctta ataccaagcc
    15361 taaaaaaagc aacccaaaaa caattttttt catttttaat tcctcacttc tatgatatga
    15421 tttttttgta ggatcttaga aataaggttt ttagtccgtg ttgcagcacg gacttttttt
    15481 acattatata agaaagcgat aagcgctttc tggaagtccg taaagattct ttaattcctc
    15541 aattcgttta ggatattgat cattgtcttc tttgtataga gaaacaatga gattagcagc
    15601 aaagcaatta gcttcgcttt cagatttgct tctagatgtt cttgttgata catagtaact
    15661 ggataagcca cgatgaaaaa tagcgtgacc taattcgtga gcgcaaatgt agaatctttc
    15721 ctcagagtct ctcagttcat catttaagaa gattattgca cgacctctaa tttcttgaaa
    15781 ctgtcctttg ggattctcga taaaaggaac gtattgaatt ttaatgccca tcttttcaca
    15841 aatataaaaa ggattagcgg actggtattt ccgcttcaac tcctcgacta aattaatcgt
    15901 atccatctcc ataagctcac atctttttgc ctttttcttt gtcttctttc acaatatccc
    15961 agaaggttcc aataaggata tcttttacgc gttggacctg ttcaggcgtc agtgtttctc
    16021 cgccataaga catattgaca tttgaatcta gtaatttatc aagttcaact acttcatctt
    16081 ttgttgccca gtcaggaact tggtttctac cgagtaaata atcagtagtt acattaaaat
    16141 aatctgctac aagttgtagt cttttagtac taggagttct atttttccat tgataaatcg
    16201 tgtttttagg tatatttaat tcttcttcca attgagttat acttatgcct cttttatgag
    16261 ctaattcctt tattctatct agtaaattca ttttcgcacc tcacaagcta cgaaaacact
    16321 aataaaaaaa ttagttaaag gtgttgacaa ctaataaaag tgttagtata ctgttttcgt
    16381 aagctaaaat attagctaaa aagacttgat aactaataaa cacttcacgg tcggcaaact
    16441 ctgaaatgta aattactagg catttctgtg tcttatttag ctatgtctat atactaataa
    16501 aaatattagt tattgtcaac gaatattagc taatttttta gcttacagat tattttttta
    16561 gaaaggagct atttttatgt ctgagaattt agacttaaaa attcgagcgg agatgagaaa
    16621 aagaagaatg actttcaaag aactagctgc gcttatcggt atttcaggag cttatttatc
    16681 agatattcta aacggcaatc gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa
    16741 aaaaatattg gacatccgat aggggggtag gcgatgacaa aactaaaaaa acaagatttt
    16801 gtaaaaaaat acaattattc tccatctact tatcaacgtc gaatgtcgga actaaaaaat
    16861 acagcaattt tctcagcggc gtatgaacgg gtcacaggac aagaagtttg gatcaataca
    16921 gaattatacg ataaattttt gtctttcaaa tcctataaca ggttacgcac aagaaaggta
    16981 acacctaaag aatttatcga gaagcattta gttgatttat aaaaaacaga acattttgag
    17041 aggtgaaggt taatgggtaa attcaacaga gcattggtat tcagcgcacc gctaatcatc
    17101 tacgctttag gactttgggg aagcaggcaa gcgttgatag gaacgattgt ttacatggtt
    17161 tggattttta tggggcttga tgaagctgaa gctgagtaca gagcgaaaaa gccaaccgag
    17221 ggggctgact aaaaatgaat agaaaagaga aactagaatg tatattacta ttactcagtt
    17281 taattctttc actaatttct ctattgggaa gtttgtattt ttgatatcaa caattttgtg
    17341 gatttgggtt ctatttagag taaatatcat ttttgtttgg tgattaagga gtttattaaa
    17401 attaattttc cctacttcaa atgcaaggta acaagatata gcacttttag gaggaatatt
    17461 tagcggtaca gcttcagagt atattctatt atcatcgact gatcctcctt tagcaattaa
    17521 tttcttaaat ggagtagctg accatctgcg gtttaagtta gcggatgaaa gttctaaatt
    17581 aactaacata gctggttctg ttgaatagtt agaaatgatt acttttgtat aaatcatatc
    17641 tgcagcgaag taagaagcat taagttcaac ttctagttga gggcgcattc cttttatttt
    17701 tatccctgtt aaaaacgtac tcaatataaa acttattatt gacatccatt gaaaaattgt
    17761 taagtgaaaa aaattcaaaa taaacaccac cagtttttaa ctaaattata ccaaaaagga
    17821 gagaagaaat aatgcaagaa ttagtaattt tgaaaaataa agaagctgtg actacgagct
    17881 tacaagtggc agaaagcttc gagaaaaaac atcaacatgt tttaagagat attgatgcac
    17941 taaaaaaaga tgtgtccaat tttggacaga tgtttgtaga aggtaatgaa ccagattcat
    18001 atggcagaaa tcgacgagtt ttcttcatta gtagagatgg atttttcttg ctggctatgg
    18061 ggtttacagg aaagaaagct atctacttca aacaaaaata cattgaagca ttcaacgaaa
    18121 tggaagatgt tattcgcaag aatactgttc ctcaaacaat tgaagatatg atgatctatc
    18181 aattagaaga aatgaaagat gttaaaaaag atgtttccat gcttaaagat actatgcgaa
    18241 ttagcggaca acaagagttt gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc
    18301 tagggggaaa agaaagccga gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt
    18361 ggtctgaatt taaacgtacc ttttcaatcc caagatatgg cgagttacct cgtaagagat
    18421 tcgatgatgc tgtttcattt attgaaatgt ggttgccaga aactgcgatc cgtatggaaa
    18481 ttgatcaact gaacagacaa cagagacttt ttggtgatga caatgaatag agctgaagcg
    18541 ctaagaatag ggacggcaat tgctaattgc tggtggaaat actataaacc aatcatccta
    18601 agccaacaac atattgacaa gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg
    18661 caagcaaaga gtcacaaaga aaacacatca taaggagatt ttagcatatg gaaaaagaac
    18721 tttccactct agatcaatat ttgattgatc ctgattgggg caagccgaaa attgaggaaa
    18781 caagtggtcg aaaaatcaga cgaaatcttt tgacgaatga agaactagct tgggatcaag
    18841 atgatttagg caaccatgta actatttggg atcatgttta tcttatccat ctatcgaagc
    18901 attcgaataa acctgaatat atttacgtca tcgaagatgg cttgattgat gcgctagaag
    18961 agtacgacag agataacttg attgatatct cttattacgg accaggtaag aaatacattg
    19021 ctgaaatgga ggcagaattt gatgagtgaa atcaaaggga caacgaactt tgaaaaactt
    19081 tttagtcgta agttaaataa aattctcaag aaaaaaggaa attttgatta tttatcttgg
    19141 gctcacgcgt gggagattat gaaaaagaat gatccacagg caacggtaac tattaatgag
    19201 tacaaacact acagggttgt ttctggaact catcaagact ttcttgttga ggaatataaa
    19261 ccttttctta tggatgaaac tgggacttat gtatctgtct cagtaacggt taaaggacac
    19321 acggaaaccg a
//
LOCUS       DARBEG010000049        19126 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000049 DARBEG010000000
VERSION     DARBEG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.049"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..703)
                     /locus_tag="SCK20_001739"
     CDS             complement(<1..703)
                     /locus_tag="SCK20_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1145789.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="SCK20_001740"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="SCK20_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="SCK20_001741"
     CDS             1758..2837
                     /locus_tag="SCK20_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1145790.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="SCK20_001742"
     CDS             2869..3462
                     /locus_tag="SCK20_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1145791.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="SCK20_001743"
     CDS             complement(3572..3799)
                     /locus_tag="SCK20_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1145792.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="SCK20_001744"
     CDS             complement(3902..6490)
                     /locus_tag="SCK20_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG1145793.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="SCK20_001745"
     CDS             complement(6510..7169)
                     /locus_tag="SCK20_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1145794.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SCK20_001746"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SCK20_001746"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG1145795.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="SCK20_001747"
     CDS             complement(7942..8808)
                     /locus_tag="SCK20_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1145796.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="SCK20_001748"
     CDS             8917..9558
                     /locus_tag="SCK20_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG1145797.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SCK20_001749"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SCK20_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG1145798.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="SCK20_001750"
     CDS             complement(10999..11898)
                     /locus_tag="SCK20_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1145799.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="SCK20_001751"
     CDS             complement(11900..12697)
                     /locus_tag="SCK20_001751"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG1145800.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="SCK20_001752"
     CDS             complement(12675..13379)
                     /locus_tag="SCK20_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG1145801.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="SCK20_001753"
     CDS             13510..14085
                     /locus_tag="SCK20_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG1145802.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="SCK20_001754"
     CDS             14203..14880
                     /locus_tag="SCK20_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1145803.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SCK20_001755"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SCK20_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG1145804.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="SCK20_001756"
     CDS             complement(16943..17941)
                     /locus_tag="SCK20_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG1145805.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="SCK20_001757"
     CDS             complement(18167..18820)
                     /locus_tag="SCK20_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG1145806.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DARBEG010000050        19070 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000050 DARBEG010000000
VERSION     DARBEG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.050"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1472
                     /locus_tag="SCK20_001758"
     CDS             <1..1472
                     /locus_tag="SCK20_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1145807.1"
                     /translation="EKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            1507..2589
                     /locus_tag="SCK20_001759"
     CDS             1507..2589
                     /locus_tag="SCK20_001759"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145808.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            2633..3805
                     /locus_tag="SCK20_001760"
     CDS             2633..3805
                     /locus_tag="SCK20_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1145809.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            3792..4496
                     /locus_tag="SCK20_001761"
     CDS             3792..4496
                     /locus_tag="SCK20_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1145810.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            4501..5661
                     /locus_tag="SCK20_001762"
     CDS             4501..5661
                     /locus_tag="SCK20_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1145811.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            5737..6015
                     /locus_tag="SCK20_001763"
     CDS             5737..6015
                     /locus_tag="SCK20_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1145812.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            5996..6367
                     /locus_tag="SCK20_001764"
     CDS             5996..6367
                     /locus_tag="SCK20_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG1145813.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            6383..6787
                     /locus_tag="SCK20_001765"
     CDS             6383..6787
                     /locus_tag="SCK20_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145814.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            6784..7194
                     /locus_tag="SCK20_001766"
     CDS             6784..7194
                     /locus_tag="SCK20_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145815.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            7255..7842
                     /locus_tag="SCK20_001767"
     CDS             7255..7842
                     /locus_tag="SCK20_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1145816.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            7915..8382
                     /locus_tag="SCK20_001768"
     CDS             7915..8382
                     /locus_tag="SCK20_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG1145817.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            8428..8736
                     /locus_tag="SCK20_001769"
     CDS             8428..8736
                     /locus_tag="SCK20_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145818.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            8931..15665
                     /locus_tag="SCK20_001770"
     CDS             8931..15665
                     /locus_tag="SCK20_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1145819.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            15669..16541
                     /locus_tag="SCK20_001771"
     CDS             15669..16541
                     /locus_tag="SCK20_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG1145820.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            16538..17587
                     /locus_tag="SCK20_001772"
     CDS             16538..17587
                     /locus_tag="SCK20_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145821.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            17591..18415
                     /locus_tag="SCK20_001773"
     CDS             17591..18415
                     /locus_tag="SCK20_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145822.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            18433..>19070
                     /locus_tag="SCK20_001774"
     CDS             18433..>19070
                     /locus_tag="SCK20_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG1145823.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         6836 a         2853 c         4031 g         5350 t
ORIGIN      
        1 ctgaaaaaaa tttttttcct ttagctaaat atcaaaaatt tatcacacct ttcattttct
       61 gctatcaaaa agaggacgac gaggtgtttt ttgatgaaat tctaaatagt attgcgcgag
      121 gcggcgggaa aaatgggttc atgtcagcaa gagactcatt ttttattagc cctctctacg
      181 gtgtcagaaa ttatgatgta acgattacag cgaattctga gaagcaggga aaggtatctt
      241 ttaaagaagt ttatgaaatg gtacaaatgc gcaaactgga aaaacaattt tatttgacta
      301 agatggctat agtcaacaat gtaactaatt cgatttttag ttataggaca aacaatccta
      361 agactatgga tagtgcaaga gatggctgct tagaatttga tgaaatccat cagtttgaat
      421 cttcagacct agttgatatt cagcgtagtg ggctagggaa aataaaacat cctcgaactt
      481 tttataatgg tacaaatggt catgttagag aaggttttta tgacaaaatg ttggaacgag
      541 cacaaaagat ttttagtgga gagaacaaga atgatcgctt gtttcccttt atttgtaaac
      601 tggataacat tgatgaaatg gataatccta aaatgtggtc aaaggctaat ccaatgttcg
      661 aagaagattc agcatattca aaacgtctgt tttctacagt gatgaaagaa tataaaaaac
      721 tagaagatga gccatcaggt aggcgtgaat ttgttgttaa acgaatgaat tttacagaag
      781 gtgatgcaga atcagatatt acgactcatg aaaaattaat ggctactaaa caagaattgc
      841 caaatttgaa tggtcgttct tgtgtggctg gatttgatta tgcaagtatt cgtgattttg
      901 caacagtggg tttgcttttt aaaattgatg ataaatttat ctggcagcaa catagttttg
      961 ctaggaaaaa gtttctagat acattcaaac tgaaagcgcc tattaaggat tgggagaaaa
     1021 aaggtttagt gacaatcgtt gatgagccat caattgatcc tcgtcattta attgattggt
     1081 taaatgaaaa aagaaaaatc catcaaatag aaatcgtttg tgccgacgga tttcgtatgg
     1141 atttgctgaa acctcttcta gagaaagaat catataattt tgagtttatc agaaatataa
     1201 gaggtgtgca gtcaaaagtt gctcctatta ttgaagatgg ttttgcaaat gaacggttta
     1261 tttttggtga tgatcctatg atgcgttggt atacaaataa ttcgtttatc aaagaagaca
     1321 aattagggaa taaaacattt ttgaaaaaag agccagtaag gcgtaaaaca gatgggtttc
     1381 atgctttttt ggcagcttta tataaacgag aagaaataac tgatatggat tatgaggaag
     1441 catttgatat gttggatgag attgaatttt aaggtatact ccttattgaa aggggtagtt
     1501 atagaagtga aaaagagata ttttctattt gttacaatat ttataataat agcaatttgt
     1561 gttttgacag tatttttacc acaaattact gagtatttaa attatcggat atttaccaca
     1621 aaagtagatg atagcgttac tagtttaagt aggctagaat ttataaaaat atgtgcatct
     1681 gtgtttggtc ctattttgac tgtaatggta tttgtgaata ctttaaatat gcagaaaaaa
     1741 acagagcaaa agcaaaatga gcttgaagta aagcaagcta aggctgagaa cgagagagca
     1801 aaaaaagagc tgttaacaca gatagatcgt gagttctatt ttttattaaa tatgtttatt
     1861 gaaatacaaa aagatcaaaa tgccaaacaa gctgttattg ctttacagaa gttggcaatt
     1921 tttaacgaaa agggttttaa tcaagactat gcatttactt ctaaagattt atcagatagt
     1981 gaaattggga gtacaattga acaatcagga ccgctgaagg gcacagttga atatataaaa
     2041 tttgaaacat ttgatgtaag caagtcgatg gggaattatc aaacaaaaac aaaagacgtt
     2101 gctacaagtt tacacgatca gaaagtagct atattgaatg aacaatatga aaagatgcaa
     2161 atatatcttg gacggtactt caaaatgttt caccgaatag ttaaaacatt aaatgagtat
     2221 tatgacgatt ataatgattt tgatgtgaag aggtatacaa aatatatagg gacattaaga
     2281 acacaaattt ctccagctga attccaagtg attttattca attctctata tataaaacga
     2341 ggttttggct taggaattca acttattgga agtggttttt ttggagatga ttttgatttt
     2401 gaaactaatc aacattttga aacaagtatc aatgaacagt ggtttctatc actatcaact
     2461 gttgacagtg ataactctat aaagcgacaa aaattatctg aatatattaa agaattagga
     2521 tccgtagaat ataaaaaaat agctaatttt gaaagtttat ataaactctt taatgaaact
     2581 aagctctaga aatagggctt ttttattttt ctttgaaagg tggtgaaaac atgtgagctt
     2641 atttgatatc tttaagcagt caataagaaa tgaagagcct tctgagtgga taccggattt
     2701 tgtatatgga gaggaagtat ctgctcgggc atacctcaaa attatggcaa aaaatactgt
     2761 tttagatttt gttgcaagaa ctatgtcaac gttagaagtt aagtttaaaa ataaagatgg
     2821 gacatcagac tgggattata ttctaaatgt tcgacctaat tctgatatgt cagcagctac
     2881 attttggcaa aaattttttt atcggctctt agatgacaac gaagtgttag taatatttac
     2941 agatgataat caattgctga ttgctgatga ttttagtcga acggagtatg ccgtgtatga
     3001 tgatgttttt acaaatgtga tggtaaaaaa ttttgtcttt gaacgttcat ggaacatgtc
     3061 tgacgtcatt tatatggaat ataataacga agaattagaa aggtttacac gaggtttatt
     3121 tgaagattac gccgcgttgt tcggacggat tctagaagtt gcaatgcgta acaaccaaat
     3181 tcgtgggtct gtatccattg attcaacggg aacggcgaat gatgaaaaag gaaaagatgg
     3241 taagactaga tctcaaagat tacaagaata tattgatagg atttggaact cttttaaaac
     3301 taactcagtt gcaattgttc ccaaagtgaa aggctttgag tatgaggaat acaccaataa
     3361 acaaggcgta tcgaatcaat cgctagatga actgaataag atgaagtcgt ctttaattga
     3421 tgatgttgcc aacgccatag gagtacctac ggcgcttatt tatggtgaaa aagctgaact
     3481 ggattcaaat attaaagctt ttcgtaaatt gtgtattgct ccactagtta aaaaacttca
     3541 agacgaattg gtggcaaaaa taattagtcg tcaagagtac aaaaacgggg aacgtataga
     3601 agttaaaaaa gttctccaaa aagatctatt agagtatgct actcaaattg ataaaataac
     3661 atctggtggt gcgttttata ttgacgaagt tcgtgatgaa gttgattatg atgagttgcc
     3721 aaataatgaa ggaaaaattc gtattcgtac gaaaaactat gaagaaactg tgaagggagg
     3781 tgagaacgac aatgccgaaa gctaaaaaag taccatttca gtttactaac gaaattcaga
     3841 atggtaagca cattctcacc ttgagtggaa atgtacagaa aaaatattgg cgtgatgatg
     3901 atgtcataaa cgctaaagat attcgtgact ctctagatac tgtaaccgat gatattgtaa
     3961 tcaagttgaa cagcccaggc ggtgatgtgt ttgaagggat tgagatttac aattatttaa
     4021 aagatcaccc atcaaatatt actgtagaag ttacagggct tgccgcaagc gcagcaacgt
     4081 ttattgtcgc tggtgccaat gatgtgatta tgaatgttgg gacttctttg atgattcatg
     4141 aagcttctac atttgcttgg gggaataaac aggacatcca aaaaacgtta aacgcattgg
     4201 aaacgatcga tgattctatc ttagctattt attcagataa gactggccaa tctgttgata
     4261 agttgcgtga atggatgaat gaagaaaaat ggtttacagc agatgaagct gtcaaatatg
     4321 gatttgccaa ttctgtgaag cgtgaagaac cacaaaatga acctcaaaat attgcagcga
     4381 tgattcaaga tgctgttgcc gttgcaatag cgaatttaaa ggaacctgtt actaataaaa
     4441 tcgaacaaga atcaaaacaa aaatcattaa tcgcacgatt gcgaaaagga gaataaagat
     4501 atgttaaaaa ttacagataa aactgcagat gcaaaaaagg catttaatgc tatttcttcg
     4561 aaagaagatg caacacccga acaaattaat gctgctctag aagaatatgt aaccgccatt
     4621 gcagaagatg ccggcaaaca ggtgcgtgct gaatatgaag aattgaaaaa tgtgacggac
     4681 aaccgtgtat tagaggctcg tggaattcct acactaacag cagaagaaac aaagttttat
     4741 aatgaagccg taaaatctgg aggtttcgat tctgacgtag tttggccaga aacgattttg
     4801 gaacgtgttt ttgaaaatct tcaaaacgat catcctattt taggcctcat taactttaca
     4861 ccaactgtag gtcgtgtcaa agtaattaga gcacgtcgta aaggtgttgc tgtttttgga
     4921 ccattgcata aagaccttga aggccaacta gatgctgaat ttggtgcaac tgaatttgtt
     4981 caacttgcgt tgaccgcatt tttcttaatt tctaatgata ccttagattt aggaccacga
     5041 tggattgacc gttttatcaa tctttcttta agtgaagcag tacgtgatgt ttgggctaaa
     5101 aaaattatta caggttctgg taaagatgag ccaattggat tgcttaaaga tctagatggt
     5161 gcggtcacaa gtggtgtata tccagataaa gctagcgcag ggacattaac ttttgcgaaa
     5221 gagaagattg ttcttgaact tgcaggggtg atgaaaaaat tatctaaata cacatacaaa
     5281 gtagatggtg acgatgaagg tgaagtgaaa taccgttctg ttgctggacg agtgtatcta
     5341 attgttaatc ctgttgatta ctgggatatc gtagctagag ttacatttgc aaatttaaac
     5401 aatgtgtatg ggactacgat gccatttatc gctgttgatc atatcatcga atctgtagac
     5461 gtaccagaaa gtaaatgtat tgcatacgtt gataaagagt atgaagctac tcaatcaaga
     5521 gctgaaaaag tctatgtata taaagaaact tttgcaatga aacgcgcaac tttgtatgct
     5581 gtcgacatgc ttggtaatgg ctatccaaca aataatgatg cagcacaagt atatgacttg
     5641 gactttacag cgggggagta atgtcccctg caattggtga gataacaccg acgactgatg
     5701 gggcagtcat taatctttcg taaatggggt tagagtatga acactgaaga atttataaaa
     5761 gaatataagg cccgatttcg tattttccat acttccgaag atgaggatat cgggaaacag
     5821 ttagagagcg ggtttgccga tataaaatct ctaatcggca gttttgatcc ttccacttat
     5881 gagaaaggaa aagaattagt ttatgaacgt actcggtatc tacggaacga atcactagag
     5941 tacttttacg acaattttca aacaatgatt atggatgcat caattgatct ggcaggtgct
     6001 ggatatgcca attaaatcaa attataagaa gcctaaaatt gtagctggtg atctaaatac
     6061 gccagttact ttttttgagt ttcaacctaa taagggtcca gaacctggtg atgaagaaaa
     6121 aaaggaactg tatttttgta cagcactagt ttacaaccca tcaatgaaag atcgtgacat
     6181 tcttggcaca aaagaaggcg ttacgataaa aatacgggat ccccatcaat cttatattcc
     6241 tactaacaaa cataaagtaa ggatattaga ttatcgatat ggagataaag tttgggaaat
     6301 aatcgatgtc tcaccggatt tggaagaaaa ttcaattgta aagatagtat tagaattaat
     6361 tacttaaatt tggtgataaa tgatgagtaa cgtaaagttt gaaggcgttc aagaaacaat
     6421 ccaagcttta gaaaaaaagt atggagaaaa gaaaactaag acattaacaa aaaaagcaat
     6481 caatgttggc gctgaaaaag tagaaaaaca gttgcaaacg gacatgaccg tttttaaaga
     6541 caaaggttat acgatcgctg aggttgttcg caagaacgct acgtatagag attacaaggc
     6601 agaagctgaa atcggttgga atggtccaca tcaacgatat agaatcattc atttgaatga
     6661 atggggatat acacgaaagg gcaaacaaat tagaccgaga ggatttggtg taataacaaa
     6721 atctcttaag aattccgagc tgttgtactt tgatgctgta ggagaggagg ttaaaaagag
     6781 tttatgaaag atatgcttaa tatcatttac gaggctcttt gtttgaatga gtatattcat
     6841 tcaatgacct acaacgagaa taccgagcaa tatcgtatta aattttatga gcaacctgaa
     6901 accgccgata aaacaggtcc aatgatcaca attcgtcctg tagatacacc taacgaagcg
     6961 taccacggaa gtaacaaaga gctttctgtg gagcatatga tacagattga cgttgaatcg
     7021 aaatatagag caacatgcaa gcagattcaa tatgagataa aaaaagagat gaaaaaacta
     7081 ggctttggcc aaatttccgg tcaagggtta gatgaatact ttactgaaac aaaccgcttt
     7141 gtagacgcgc gtcgttacga aggaaataca aaaatttacg ataccgaata ttagaaatta
     7201 agacacgaaa ataacgtgtc tttttttatt acccaaaatt aggaggaaaa ttttatgact
     7261 ttagtaggat tcaaaaaaat gacaatcggg gtctttgaca gcacagggaa gattccagca
     7321 gcaaatttat atgtaattga aggtgaacaa aacaaagggg cgactgtatc tgctgaaatc
     7381 agtgggctat caaaagaacc atcaaaagtt tacggatcga acatcgctta ctatgtctct
     7441 caaaaaggaa caggcgatgt ttcagctact tttggattgt tagatttacc aacagaagta
     7501 aatgacaaaa ttcttggtta caaaaccgat acaaacaaaa tcagcttctt gggagaagat
     7561 actgagccac catattgtgc aattttgatg gaatcggagg atttgaacgg cgatacagca
     7621 atgcttgcga tgttcaaagg aaaattcagt cgtgaatcta ttaatcttaa cactacgaca
     7681 aatgaagctt tcgaaccaga agcagaagaa tatgtatttt cagcgattgc caatgatgtt
     7741 gaaggtgatg ctaaaggcca aacagtcgca aaatatgttg gagacgaaga agcttctatt
     7801 actggtttga aagagatggt atttcctgca gcgggggagt aactagcccc gttgttggag
     7861 caatcactcc cactattaat ggggcaacag ttgaattaag ttaggaggat aactatgcca
     7921 gatacattta aaatctataa aaaaaatggt actgaattta caaaagtcgc agagggtgaa
     7981 agtccacttt ctattactgg aattgcagca aatactcaag ttgctaaagg agattatcaa
     8041 gctacgcgtg tggtcggagg tcaagaatca gtaaaagtag atattcctgc attcaccact
     8101 cttccaattt cagttactgg tgtgacgttg gataaaacga cagctgaagt tgaacaaggc
     8161 ggaaccttaa aattaactcc tactgtaaca ccagcaaatg caacagacaa aacaggaagc
     8221 tggagcagtt cgaatacagc tgtagctacg gttactggag gcaacgttac agtaaaaaca
     8281 gacgctgaag ttggcggaac tacagaagtt tcatttacta ctacggatgg cggtaaagta
     8341 gcaaaatgta ctattacagt tattgaaaaa gcagcgggct agtcttcgga ctagcttttt
     8401 tatttggaaa ataatggagg aatctaaatg gcaaaagtaa gaattgaatt gaaagaagaa
     8461 tcaggaaaga aagtctatga aaacatagac acaacaggga aagattatcg aaaagcttta
     8521 gaaacaatca aaaagctaaa tgaagacgga gtaatgattt ggaatcgttt agacatttat
     8581 ttggattttg ctgtaggtat cttcagggat agcaaattga cagctgaaca aattcttgat
     8641 ggcttaccat ctgagaaggt aatgagcaca ttggacgaca tcctaggaga agtaatgggg
     8701 attgaaagta atccagaccc agacgcaaaa aagtaacacc cgaagaagca gaagaaatgt
     8761 acttaaacct atgcagagag ttagtgaaag cgggatggtc actatctgac atagaagata
     8821 attcctttga aatgatgatg aaaattgcgt gcacttcgcc taagaaagag aaacaaaaag
     8881 aagttgatct taaagacttc ttgaaatcca tgtagagagg aggtaaacat atggcaaatg
     8941 gaaaaccttt aggcaatatg attatcaaac tagacttaga cagttcagct ttttcaaaag
     9001 gtctagccgg agctaaaaac gctgttaacc atcaaatgaa agcaatgaaa tcacagatgc
     9061 aagtaatgaa ctcttcagga aattctctcg gtgctttaca agcaaaatac aatggtttag
     9121 gcggagttct acaagcaaat gaaaagcaag tagaattgct cactaaggct tataaggata
     9181 gttttgactc taacggaaac gctactgctt cgactgctaa atacgctaat caattgaatc
     9241 aagcaacagc tagatcagca agttacgaat ctcagatgaa aacaactgtt ggacagattg
     9301 ctcggatgaa agtggaaacg gaagggttta cggggaaact aaaggctaat tctgatgtat
     9361 tgattaattc tgggaaaaaa attgaagcct ttggtaaagg agtttctggg ataggatctg
     9421 ccctaactgt tggagtaaca gctccaatag tggcaggcgc aactgctgtt actagtgcag
     9481 ctatttcatg ggaaagtgct tttgcaggtg taaaaaagac aaatgatgaa gttgtagact
     9541 cgaatggaaa cgtagtttat tcctatagcg acttagagaa aggtctgcgt aatcttgcca
     9601 cccaattacc tggtagccat caagaaatag caaatgtagc cgaagcagcc gggcaattag
     9661 gtataaagac tgaaaacgta gttagcttca ctaaaacgat gatagacatg ggtgaatcga
     9721 ctaatatgtc ggctgaaact gcggcaactt ctttagcacg atttgctaat attacccaaa
     9781 tgtctcaaaa agactttgat aaattaggat cagtcattgt tgatctaggt aacaattttg
     9841 ctacaaccga atcagaaatc actgaaatgg gattgcggtt agcgggagct ggtaaacaga
     9901 ttggaatgag ccaaggcgaa attctaggat ttgctactgc tctatcttct gttggtgtcg
     9961 aagctgaagc gggtgggtct gccttttcga aggtcatgat ccagatgcaa ctagctgtag
    10021 aaaaaggatt tggcgctttt gatcagctga aacagatggc agaagaacaa ggtgttccgt
    10081 gggttaattt agtaaatgct gtgagagacg gtggaaaatc gttaaaagca gtatcagagc
    10141 aaatgggatt cacttcatct gatttaaaga aaatgtataa agaagcggac aattcgaaaa
    10201 gttcgcttga aaattttgcg aatgtggcag gtgttacaag tgaacaattc cagaagttgt
    10261 tcaagagtga tccttcagaa gcaatcatca agttcattca agggctgaaa gattcagaaa
    10321 aacatggtac ttcagcaatc aaagttctgg atgacatgga tataaaagaa gttcgactac
    10381 gcgacagttt attgagagct gcaaatgcta gtgacatatt caaaggagca attgaaaaag
    10441 gaaacggcgc gtggaaacaa aatacagctt taacaaatga agctaataaa agatacgaaa
    10501 caactgaatc aaagctaaag atattgaaaa acgaagtgat tgatacagca atcgatcttg
    10561 gaggtccatt tgtagacgcg ttgcgtgatg gtctaaatgt atcgaaacca ttgataaaaa
    10621 acttagggaa tatggcgaaa gctttctcag ctatgagtga tgatcaacaa agaaacatta
    10681 tcaaatgggt tggattagca gcagcagctg gtccagcgct gaagctttta ggaactggta
    10741 cttcagtaat aggtaaagtt acttcaacta ctggaagatt gacgaaaagc cttgttgatt
    10801 tagcagcgaa agcagcagaa aagaaggcta tggatgcctt tgcatcgtcg gtaacaacag
    10861 ctggtgctgc agctgcaaag acagcaggag ctggaggtat tggcgggtta gtccctatga
    10921 tgggaaaaac agctagtgct gcaagtactg ctgcgggcgc cagcggaata ggcgcaatga
    10981 caacgtcact aggtttgtta ggtcctgctc tacttggtat tgttggcgtt ggtggcgcac
    11041 tggcagttgg ctatggagca tggaaattat ttggtgaaga agcttggaat tcctcgcaaa
    11101 gagtaaagca atggggaaca gatgtcggtg aggagataga tagtactctt gacggagtac
    11161 agaagaatat tgaaggtgca aatggtcaat tcggtcttct caaagagggt tttactgttg
    11221 atgatgcaaa taatatggcg gctaatttcg caaccgctgg aacatctttg gaaacatctt
    11281 taacgggtag aataaaggca ttagacgatg cactaaaagg gctacctcaa tcagttcagg
    11341 atgccatgaa agaagtagct gaatcagaag aagaaacctt agggcaatct ctttctaaaa
    11401 taaaagacaa caatgaagaa ataaaaaaaa tcagagaaag agccgcaaat cagaacagag
    11461 acttgactgt ttcagaacta caacagatac aggatctaat gaaaggaact tctcaagcgt
    11521 atgtggatac gctcgatgtt tctgctaaag acagaaaaaa gatactatca gccatgaatg
    11581 gtgatgttgc acaagctaca gaagaagaag cgaagacgtg gttacaatct ttagcgaaac
    11641 aaaaagctgc aactaccgaa aattatagac aaatgcttga agaaaaaaag gcggctttaa
    11701 aggaaaaggg agtaagcaaa gaattcattg atgcattaga agatgaaatg aatgattaca
    11761 tcaaaactac aaatcaagga ttcgatagcc aaatagctgc tataacaaat aaatatccac
    11821 aattgattaa cgaagtgagt ttatcaaatg gtcaattatt tagcaaaacg gcagctgcta
    11881 tggacaatac tggtcagtat acaaaagttt ttctcgaaag taatgaaaag attttagccc
    11941 aagcgaagaa aacatcggat gaaatagcag ataatgccga aaaaaatgca gaacaacttg
    12001 gtatggtagc tgatgaggct acgcatgctg gatcaacttg gaatggctta gttttagacg
    12061 aaaaaacagg aaaagtaaaa actaatgtac aagaagtagt gaatgaagca acgaaggatt
    12121 tcactaaatg gaatgaattg aaacctgttg ttcatgatgc gaaactaaca acgaatgcaa
    12181 aagatgtcat ttccatagcg gcagtacaaa atggctattg ggatgatatg gaatggaaag
    12241 aaaaagaagc attattagaa gatgagtgtt ctaaaaatgt atacaaggct ttagaaaatg
    12301 ctggtaaatg gaatgagtta gatattcctt ctaaagaagc aatcctaact tcaaatactc
    12361 ctgaagttat gggtgaaact ctcatgaatt tgggattatg ggatacttac aaacttcaag
    12421 tgaaagattt agatttgaat gcgtataaag tatacgatgc tatcgatgga tcagaagaaa
    12481 aattaaatag ttgggctcaa ttagataacc ccacaaaaga attgttatta gataactcag
    12541 atttttctac aaaacttttt aattccgaag aaatgtttaa acgttggaat cagttatcat
    12601 ctaatgcaaa gtttctttcg gctaataata ctaaatttac agaggttctt ttatcatctg
    12661 acagaatgtg gaatcaatgg gtaaaccttc ctgataatga gaaaagatta cttgcagata
    12721 acgaagattt agctaccact gttttatctt cagaagaaat atacggaaga tggactcaat
    12781 taccagataa tcaaaaatat atgttagctg acaatactga cttagcaaac aaaatattcg
    12841 cttcagaaga atcatggaat gcatggaaag caattccaga cacagttaaa aacttgcttg
    12901 gtaataatat tgatttactg gctaaagtga aagacgggac tgtaagtgta gaagaatata
    12961 acaaagttat gccacttctt aaaactctat atggagataa cgttaattta aaagaggcaa
    13021 tcagtggggc taaaagctct atagagaatt acaacaaaaa tatttttcct agtgataaaa
    13081 ctgcagtagg acacgatgag gcttcacaag ctgctaaaga tgcatttgaa gcgttcaaca
    13141 tcttccaaac aaaaatacca gataaaatta ctaaaactgt atcagctgac tttataggtc
    13201 ctatgccgaa aaatgcgaaa ggaacaaact tccatcctgg cggagcagct atggtaaacg
    13261 atcaaaaagg gcctacttat gaagagctta taaccttgcc aagcggagaa acttttatac
    13321 ctaaaggacg taatgtcgtt ttagacttac caagaggttc gaaagtactg aacgccacta
    13381 aaactaaacg tctagtgcct aaatatgctg atggtatagg aaacataacg acggtttcat
    13441 ctacgctaaa cttagatgct ttaattttag ctattaacga gctaacgacg gtattgagaa
    13501 ctcaactgat ggcaggaacg actgatgcta aagatactaa actatcagat gaaacaaaag
    13561 ttgccgatcc gattattccg gacagtcttt ctgaaaaatc agatcagtat ctaggaattg
    13621 gagctcaatg gttaactaat ctaatgaatg gttggaactc agcggttcct caatatatga
    13681 atagtgaaat ggtttttatt acaaactatc tcaatgcgct aaaacttcaa aacaatccta
    13741 attatacgca aggagcaaca tggaataaga acttactgaa tggatggaat agtttaactg
    13801 ggacctttat tgctacaatc aattcatttt gtaatcaagc aatggtcacg cttagaaact
    13861 acaacactcc tatgtacaac aatggccgaa cgtggcagca aaataatctt aatggttgga
    13921 attcattgta tggatcattc atagcccgtg taaatcagct tggtaacgat tcgattaaca
    13981 accttcgttc taaaagtggt ggtttttaca atgcagggac attcttgttg caatctttaa
    14041 taaacggaat gaattcgtta ggtaattctc tttctactac aatgaacaat gtcgctaata
    14101 caatggttgg tggtataggc aaaggagtta atggcgtcat atcgggagtt aactatgtgc
    14161 ttaaggaagt cgaatcaagt aagaatatcg gaaactggcc aattccacaa tacgctaagg
    14221 gaactgatgg acatcctggt ggacttgcta tgattaatga tcaaaaaggc ctagttcacg
    14281 aagaatatgt tcagatgcca gatggacgta gttttattgc caaaggtaga gatcttcttg
    14341 ttaatttgcc taaaggtgct caggtcttaa atgcttcttt aacaaagaaa ttaaaggaac
    14401 gtttaaatat tccacactac gaaaaaggta ttggcaattt agatattgtt gatttgttag
    14461 atgatgaaaa aagaatgtta gaattcttaa ctagcaaagt tgatttttca ggcattaatg
    14521 aaccatggct tgatatgact aaatcaggaa cgtcattaat gtccaaagct gcaaatacaa
    14581 tgcttcaatc aaaattaagt gaattcttca ctcatggcaa ttttgatggt gcagtaaatg
    14641 ccaatggtgt ttatcaatat ttagttgatg tggcacagaa agtaatgggt aagttccccg
    14701 gacttacagt aacttctgga tatcgagcag gagatgccta ctatcacgga aaacggcaag
    14761 ctatcgactt ggcttatcca ggtatttctg gagatccgag atatacagca gcagcaaatt
    14821 acgcttttga aaaattccct tcaaaaattg cgtatgtcat tacgaatgga cgtgtacgtg
    14881 accgcatggg attatctgga acaaggtcaa gcggacaatg gacgaattgg ccagatggcg
    14941 atcacttcga tcatattcat ttaaacggtt cgatggggtc tggcgatata ttcaatggag
    15001 gagttggagg aagtggtgtt gcacgctggc gttcttatgt gtctaaagca ttgaaaatga
    15061 atggattacc agctacagca gcgtatataa atgcctggat gtcccaaatc caaacggaat
    15121 ctggtggtaa tgagaaagca atcggcggca atgatgggct cgccgaaggc aatgcaacag
    15181 gattacttca aacgaaaccc ggaacttttg cagctaacgc ttttcctggt cacggaaata
    15241 ttatgaacgg atttgataac atgttagcag caatcaatta tgctaagaaa cgttacggag
    15301 ttgcaggtat gttgcaagtt atcgggaaag gacatggata tgccaatggc ggcttgatta
    15361 ccaaagacgg actatatcga gcaggagaag gaaataaacc agaaatggtt attccattga
    15421 caagaaaaac aagagctatc gaacttatgg gtcaagcatt agccttcctt tctggaagca
    15481 ataaaaaaga aacgaaaagt gagattaatt ctgtagataa tagacatatc ctacaagcga
    15541 tagaaaaaca gtctcgtatg acaaatgatc tgctatcatt attactctcg ttttttaaaa
    15601 gtaacaatcg atcagataaa gaactagctt tagatattca gaaaatttta atcaggagga
    15661 catagtattt gaggacagta atattaagaa ataaattgaa tgaagaaatt gatttgtcca
    15721 cagaagatta ttttgctact gaaatgtcga atctaggttt tgaagttaca aaagagcata
    15781 ttggacaatg ggggagtttc agagaaatag gagagagtat tgaattgtct gaatttcaat
    15841 catcagttat gatttctgtt cacggattta gagaacaaca attatatggt tcattagttg
    15901 aatttctggc taatggacct tttgaactag aattttcttt tgattcagaa acaatgatca
    15961 ggaaatgtag tttgaaatca ttatctaaaa cagaaattga ttcccaaagc tcattgctaa
    16021 cagatacttt gaatctatat tttacttcaa gctggtattc tattaagcgt gaaaaattaa
    16081 tacagaattc aacttctctc aatacacgtg gaaaagttta ctcatttgtt cgaccatatg
    16141 tctacaccaa aaatgtatgg gaaaagaaag gtgtatttaa gttcagtaat aattctgtat
    16201 atttgacaaa tagtacagag cgaatgtctc cgttaaaaat aaggattatt ggagagtgct
    16261 caaatccata ttgggaagtt atccaaaact ctaaagtagt agcttcagat ggctatttta
    16321 ttaatatgaa agacacacaa atattagaag tgtctagttt gctagaagac caaacggcaa
    16381 ttttaaaaga tgtaggtgga gttgaatctt ctgtttatca acaacaagat tttactaaat
    16441 caaacttcgt ccaagcgcct ctaggagaat tcagcgttgt ttttcatgtt ggacatgcaa
    16501 atgtagagat tgaactttat gaggagcgtg atctattttg attttagcca ttaccctctt
    16561 tcaccgagat atgaatttat ataacgaacg tttaatttcg actgagttcg aatttggaac
    16621 ggatgaaatt ggagaagaag taagtaattt tattacagat aaatatgtcc atgtaaaatc
    16681 aggagatttt ctactagcaa aatttattcc tagcgggaag attgcttatt ttggagtgat
    16741 tagctcgcaa gaagacaaaa aaataaattg caaaggatta ctcagtttgg ccgatagcga
    16801 gataccgact gtaaaaatat caggtgctaa ttatgaagag catattcgac gactgattga
    16861 atactactta ttgaacgatc caacaaaaca actaaaaaat attttggagg taaaaacaga
    16921 aagcgttact tctcattctt atcaagctgt tgacacaaat aagagacgat tgaatgcata
    16981 tataataaat gggttcaaaa aatataatgt taaatggtat gtcaaagaga ttaaaggtaa
    17041 aaaaatatat actggtattc gagcagttga tgattcaatt tatattaaag ataattcatc
    17101 tgagtttaat gactgggatg tttttgtgca atctcctgga ccagggaacg agaacaaatt
    17161 attgatcgta gataaagcaa tgaaagatat agaaaatccc ttggtattgt ctacgtggta
    17221 tttagatgac gaaaataatc ttacgcaaga ttcaacgaat aagaacattg cattgcctac
    17281 cgttaattta gtaaatatat atgatcaaac tcaagaagat agaccttctt atgaagaggt
    17341 agctaaatcg gagcttaaag gcaacgccta ttcacatgaa attaaagtta atgttgtgct
    17401 aaatgctaga aatatcaatg tagaaaatat cgaaacagga atgttagcga ctattgttta
    17461 caaagagcag acttacaaat ctgttctaac tgcctggcga atatccagca gtaaaaagaa
    17521 tatagaattg acatttggta atgtccgaag tcgttttatg gattattttg aagataatgg
    17581 gggataaaaa atggttagca atgtggatgg atatcaattt gaaaacgtga aagtaagcgc
    17641 agaaaatgat gctagacttt atcacgtttt atataatcgg aaaaatcagg ttattgatgg
    17701 ttacgatcag tctatgaatt tatcttcaag cggattaaca gtaaaagttg ctgcaggagc
    17761 agcgattatt caaggtcgta tggtcgttgt tcgacaagaa gaaagtataa cagttccagc
    17821 aaactcaagt ggttatatag cattaacagt ggatttgaca caagaagtta tacctggatc
    17881 tattcttcca gaatcggaag aatatgaatg gactaataat caagtcaagc tagaatttat
    17941 aacaaaagtt ataaaaggta atttaaacaa tggtgataag gtctataatt taccactatg
    18001 ctcagttaat tctactggat caactgtttc aatctcaaag atatcggata gttacgagct
    18061 gactctctct aaaggagaaa ttttgtggag ggggactgcg ttaatgcatg atactcaaac
    18121 tgtccaacct tcaaaaaaaa tttggcagac agtttcaggt tttttgttag tatggctccc
    18181 atacgaaaac gggcaagcaa ttgaggatag atatgtaact acgccttttt ataaggagcg
    18241 tataactttt actaatgttt taggagaaat tgtttcagga tttgatgact atcacaaaaa
    18301 gtggtttagt aaacgaataa actataattc gaatactaat atatttacag gtgcagcaag
    18361 caatgcaagt ggagataacg caaatatggt gcttaggtat atagtttctt tttagttagg
    18421 aggtatggaa gagtggcaaa tttagaaatt aaattatctg caaataagag acagccttat
    18481 ctacgtcacc gtgttgttgg tagagttggt gatggggggc tcacaacaat caatgtacaa
    18541 cttcttgagg aagatgaaat tacacctttt gtaattaatc taaacggtac tttgaaattt
    18601 gtgggcgaag tttcaaacgg taactatacc gagggagaac cagaaataat cgattcgact
    18661 aatgggttaa ttagttacac gttcactaag tcaaatttca gcacgagtca tcaattcaaa
    18721 caagcatatt ttgaatatgt agatcctaac ggcaaaaaag taacttttca gaacttcatc
    18781 atagacgtgt tagaacgagt agatattaat tcggagcaag cgaaatacta tatttcttca
    18841 ttggaaaaat tacagagtga aatgcaaacc actttcaatc agttcattag tgataaacag
    18901 gtccaatacg atcaaatcta ctcgaaatat aacgaattag taagattgat aaatgaatca
    18961 gataagcaag taaatgatcg tattgataag gcaaataatc gtattgatca aaccaatcag
    19021 caaatcggcg atctcggcaa gctgaaaaag atgtacagta acagcattga
//
LOCUS       DARBEG010000051        18981 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000051 DARBEG010000000
VERSION     DARBEG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.051"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(17..538)
                     /locus_tag="SCK20_001775"
     CDS             complement(17..538)
                     /locus_tag="SCK20_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1145824.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            737..4429
                     /gene="nifJ"
                     /locus_tag="SCK20_001776"
     CDS             737..4429
                     /gene="nifJ"
                     /locus_tag="SCK20_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG1145825.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            4618..5505
                     /locus_tag="SCK20_001777"
     CDS             4618..5505
                     /locus_tag="SCK20_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG1145826.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            5502..6671
                     /locus_tag="SCK20_001778"
     CDS             5502..6671
                     /locus_tag="SCK20_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145827.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            6695..8050
                     /locus_tag="SCK20_001779"
     CDS             6695..8050
                     /locus_tag="SCK20_001779"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG1145828.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="SCK20_001780"
     CDS             complement(8122..9927)
                     /gene="glmS"
                     /locus_tag="SCK20_001780"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG1145829.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            10336..10626
                     /locus_tag="SCK20_001781"
     CDS             10336..10626
                     /locus_tag="SCK20_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145830.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            10639..11664
                     /gene="sppA"
                     /locus_tag="SCK20_001782"
     CDS             10639..11664
                     /gene="sppA"
                     /locus_tag="SCK20_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1145831.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            11667..12326
                     /locus_tag="SCK20_001783"
     CDS             11667..12326
                     /locus_tag="SCK20_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1145832.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            12483..13415
                     /locus_tag="SCK20_001784"
     CDS             12483..13415
                     /locus_tag="SCK20_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145833.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            13569..14858
                     /locus_tag="SCK20_001785"
     CDS             13569..14858
                     /locus_tag="SCK20_001785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG1145834.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            15105..16355
                     /gene="clpX"
                     /locus_tag="SCK20_001786"
     CDS             15105..16355
                     /gene="clpX"
                     /locus_tag="SCK20_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG1145835.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            16438..17025
                     /locus_tag="SCK20_001787"
     CDS             16438..17025
                     /locus_tag="SCK20_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG1145836.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(17099..17254)
                     /locus_tag="SCK20_001788"
     CDS             complement(17099..17254)
                     /locus_tag="SCK20_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145837.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            17390..18718
                     /locus_tag="SCK20_001789"
     CDS             17390..18718
                     /locus_tag="SCK20_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145838.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            18729..18938
                     /locus_tag="SCK20_001790"
     CDS             18729..18938
                     /locus_tag="SCK20_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145839.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT         6219 a         3379 c         4083 g         5300 t
ORIGIN      
        1 cacgctcctt atttttttat tttgtttgga tcaattcttt taattcctct attgaaatag
       61 attcatcaaa gatttcttta cccaagatga cagttggaac aaatcgaata tgtgcttgct
      121 cagcttcgtt gatgacagct tggctcagct gttcgtcttt ttgttctgtc agctttagtt
      181 tatctactgc aaaatccgct acttcttgga gagaaagatg tttccactcg tcttgagtat
      241 cgaatatttg tttgatctct ttgatcgctt tttggccatc gctgattgtg agataccggt
      301 gcatcacgtt ccctcgctgt aagctttctt tttctttatc gaacaatttg atcactcgct
      361 gcaattgccc gctttggact gcttcttcta atagctcata agattcttca aaccattgtc
      421 ggcaatacgg acaagctaag ttgatgaatt cgaccatttt tttaggagca ttcgatgaac
      481 cataaaggat tcctttttgt gtatttgttt tcgttgcatc aatcactgat atatccatgt
      541 taaaacacct ctttcgctta tagtttatac atgtctgaca aaaaaaggca acgatttaga
      601 ctgtttgtct aaaaattaga tgaccaattc aaatagatac atgtaaaata tttttataaa
      661 taaaagaaaa aaatggcatg ttttacgtta taatggaaca gataataaga gagaatatga
      721 acaaggatga tgaatcatgc gaaaaatgaa aacaatggat ggcaatacgg cagccgcata
      781 tatttcttat gcatttaccg agattgctgc aatatatcca ataacaccca gttcgaccat
      841 ggctgaatta gtagatcaat ggtcagcagc aggaaaagaa aacgtattcg gtcaaccagt
      901 aaaagtagta gaaatgcaat ccgaagcagg agcagctggt gtggtccatg gatcattgaa
      961 aactggtgca ctgacaacta catataccgc ttcacaaggg ctgcttttga tgataccgaa
     1021 tatgtacaag attgctgggg aactactgcc aagtgttttc cacgttgcta gtcgggcagt
     1081 gacgacaaat gcgttgaata ttttcggaga tcacggagac gtgatggctg caagacaaac
     1141 aggatttgct atgttagctg aaagcagtgt acaagaagtg atggatttag cacctgttgc
     1201 ccatcttgct tctattgaag gagaagtccc ttttttgaat ttctttgatg gattccgtac
     1261 gagtcacgag attcaaaaaa tcgaagtttt ggaatatgaa gaattagctc ctctgatcaa
     1321 tcaggagaaa ttagcttcat ttcgtaaacg agggatgaat cccaatcatc cgactgttag
     1381 tggtacgaac cagaatccag acatccattt tcaacaacgg gaaacgatca acccctatta
     1441 tgataaatta cctgaaatcg ttcaaaaata tatgaaagag atcaaccgat tacgaggaac
     1501 ttcttatgac ttggtgactt attacggagc agacgatgcg gaagaagtca ttgtttctat
     1561 gggatcagtt gcacagacga ttgaacaaac gattgattac ttgaatgcaa acggacggaa
     1621 aacaggtttt ttgaatatcc atctttatcg tccttttcca gtggaaacat tcgtgcaaaa
     1681 gttgcctcat acagtaaaaa caattgctgt tcttgatcga agtaaagaac caggggcagg
     1741 tggagaacct ctcttattag acgttcaaag tgcgttatat gattctgaac ttcgcccggc
     1801 tgttattggt ggtcgctacg gattggggtc taaagatgtg actcccgacc aaatctcagc
     1861 agtttttgat gaattgaaaa aagacccaag catacgcaaa aaacggttta ccatagggat
     1921 cgtggatgat gtgacttatc aatctttgga accaaaggaa agtctggatt tgacagaacc
     1981 gcaaacattc caggccaagt tttggggatt tggatctgac ggaacagttg gagcaaataa
     2041 atcagctatc aagattattg gagatcacac cgataaatat gcgcaaggct atttttatta
     2101 tgattcgaaa aaatctggcg gtctgactgt ctctcatctt cgttttggag atacgccgat
     2161 acgttcagcg tatcttgtcg agcatgcgga ccttgttgcc tgtcacacac cagcttacct
     2221 gcatagctat gacttggtca aaggattgaa gccaggtggc atttttttat tgaatactct
     2281 ttggagcgac gaacaattag aaacgcatct gcccttaaaa ttaaaacgtt acttggctga
     2341 aaataacatt agattctata cgatcaatgc catgagattg gcccaagaag tcggtttggg
     2401 acgccggatc aatacggcaa tggaaacggc tttttttaaa ttagctgaca tcattccttt
     2461 tgatgaggtg cttccgctgc taaaagaaga agccctgaaa agctatggac ataaatcaat
     2521 ggctatcgta gaaaaaaaca tacaagcaat cgatcggacg accgaactgc ttcatcaagt
     2581 cgcgatccca gaccattgga gaacgttgga gatgcctgaa aaaatcaata aaaggaacac
     2641 tggttatata caagaaatac ttgaaccagt gaatgcacag gaaggaaatc aactctctgt
     2701 tggtacactt gtagcaaatg gaatgacaac tggcgaaatg cctttaggaa cggcttctat
     2761 ggaaaagcgg ggcgtcgctt tagaagtacc tgagtggatt agtgatcgct gtacgatgtg
     2821 taatgaatgt gcctttgttt gtcctcatgc tgccatccgt ccatttttag cagatgaaga
     2881 ggaaatggaa gaagcgccag aaggatttat cgtgagagaa atgcgaggcg cagacggcgt
     2941 aaaataccgt atccaagttt cagtggaaga ctgtacaggt tgtggtctgt gtgtagaggc
     3001 ttgtcccgca aaagaaaaag ctttagtgat gaagccttat gaagaacaga aagaacaagc
     3061 tgtgaattgg gcttttgcaa tgacattaag acaaaaagca aatccagtca aaggaaagaa
     3121 tactgtcttg tctacacaat tcaatcaacc gcttcttgaa ttctcgggcg cttgctcagg
     3181 ctgtggcgaa acaccttatg taaaattatt gacacaaatg ttcggtgatc gaatgatgat
     3241 cgcgaatgcg acaggctgtt cttctatttg gggcggtgct tctcctgctt ctccttatac
     3301 gaccaatgaa tgtggtcaag ggcctgcatg gtcaaattct ttattggaag ataatgctga
     3361 atttggttat ggtatgcttc tagcggcaca gactcgtcgc gaggctttgg caagttcaat
     3421 caaagaagta ttgccaaaag tatcagaaga actacgagaa ttgatggaag actggttagt
     3481 acacatgttt gagagtgaag gaacgcagca aagagcagca aaactgcaag ctgcgatcga
     3541 aagcgaacga tcaattttcc ctgaactgga ggatatatac aagagaaagg atctattcgt
     3601 aaaaaacagc caatggatga ttggtggaga cggatgggct tacgacattg gttttggcgg
     3661 gatcgatcat gtacttgcca gcggagcaga cgtgaatatc cttgttttgg ataacgaagt
     3721 ctattccaat actggcggtc aaacctcaaa agcaacacca gcttcggcaa tcgctaaatt
     3781 ttcggctagt ggaaagtatg cttcaaagaa agatctagga atgatggcga tgacatatgg
     3841 aaatgtttat gtggcgcaaa ttgcttcagg tgccaatcaa atgcaaacga tcaaggcatt
     3901 tgaagaagca gaaaagtatc ctggtccttc tctgattatt gcctatacgc cttgtatcac
     3961 acatggcttg cttggcggaa tgaaagaatc gcttaaagaa gcaaaagaag ctgtttcttc
     4021 tggttattgg tcattatatc gatataaccc tacaatggtc gaaaaaaaca agaacccaat
     4081 gactttggac tacaaaaaac ctgatttctc tgctatgcct gatttcatga ggaaacaagt
     4141 tcgcttttcc tcgctagata acgcaaatcc aaatcttgca aaagctttat ttgaaaagac
     4201 tgtccacgac gctaaaacac gtttttacaa ctatgctgca ctgacgggag aattagaaaa
     4261 aatcaagaaa cgacttgaac cagaggaatc tatcgatgta cccgttaaga ctagaaaaga
     4321 aaaaacgacg gaccttgaag cagaagaaag aagagctgct agaagagctg ccagagcaga
     4381 acgacgtaaa aaaatggaac aagctacaaa agaaaaaaag gaaaaataac agaaaaaata
     4441 taatgaataa ttctctgaag tttaaaaata tttattgcag agaaaaataa aacgcatact
     4501 tgcaattttc ttgaaaatta tgcgtttttc tcaagaaaaa tacgagcata tcagcctcta
     4561 aaattaaaac atatgatata ctagagagga aatctcaaaa gagaagagag gctttttatg
     4621 tcatttcaaa tcggggaact ttttaatttg gactattggc agcaaatgtt ttccggtgac
     4681 ttgttctctc tgaatttttt ggtgaacata cttgatattc tggttgtctg gtatcttgta
     4741 tacaaactga tccaactgct gcgaggcaca aaagcgattc agcttctgaa aggtgtagct
     4801 atttttattg ttatccgaat cgtagcggaa atcattggtc tgcacacctt gtcttggctg
     4861 atgaaccaag tcattacgta cggggtgata gctgctatcg tgatttttca gccggaaatc
     4921 cggagaggac tggaacatct tggacgcagt tcgcttttca aacaatcaaa aagcgaacag
     4981 caggaagatg agaagatgat cttgtctttt gacaaagcca tccaatatat gtccaagcga
     5041 aaaatcggtg cgttgatcac tatcgaacgt catactggat tagatgagta tattgagaca
     5101 gggattgctt tagatgcaga tatcactggg gagctgctga tcaatatctt tattcctaat
     5161 acaccgctgc atgacggtgc agttattgta aaagaaggaa agatcgcagt agcaagcgcc
     5221 tatcttcctc tatcagaaag tatgttgatc cctaaagaat tcgggactcg acaccgggca
     5281 gctgtcggta taagcgaagt cagtgacgcg attaccatcg ttgtctctga agaaaccggg
     5341 gacgtcagca tcacgttaga taatgaactg atggctggct tatcccagca agaatatttg
     5401 gcaatcttgc gtagagaact aattgctgaa gagaaaacaa aaaacaagaa aaacgtgatt
     5461 caatctttcc tagacggtat gactaaggga ggggatcgca aatgatctca aaagagaaac
     5521 ggacaagcat tctttacagt tttattgcat tgctgttcag tattgcgctg tacttcaatg
     5581 caaatggcca gtccgttcca aatacgcttt ctggaaatga agcgtataat caaacagtta
     5641 cgggggtgcc gatcaagatt tcttatgatt caaagcgcta ctatatccat ggatatgaaa
     5701 atgcagtgac tgtcaaactt tccagtgcaa accgagttca gttgaatgca gaggcaaacg
     5761 aagatacacg gatgttccgc gtcacagcag atatcactaa gctgggcgaa ggaacacatg
     5821 aagttccgct aaaaattcaa aatttgagca gtgctgtcac agcaaagatc gaaccatcga
     5881 ctattacagt aacggttgag aaaaaggtaa taaaagattt cgatgtggaa acgttgattc
     5941 cttcaacgtt cacaccatct ggatatgaac ttgatgatac ttccgttagt cctaaaaaag
     6001 tctctatcac tacaggtgac aaaacattgg cagaaatcaa acgagtagta gctgatgtgg
     6061 acgcttcgat ggtcacagat gatgggatta atagtgaggt cccaatacag gcgctgaatg
     6121 cagcagggga agtattaagt atcgtctctg atccagtgca agtaacagtg agagctgatt
     6181 cgatcaagcc aagcaagtcg gtacgacttt atgggataca gcaaggaaca ccagctgccg
     6241 gggtagaaag ctatgatttc agtttttcaa aactggaagc agaagtaagc ggctctagcg
     6301 atttgcttgc atcgatcggt gacagtattg ctgtaccgat caatgtctca ggtatcaatc
     6361 atcgaacaac tcgtaaaatt gaaataccag tagaagaggg gttaagtatc aagcccgaat
     6421 cagttagtgt ggagattaca cccgtgttgc aaacgtcaag cagtagctct gcttcgacga
     6481 gcagtcggac acaagaaaat acaagtacga gtagaagtat ttcatctgct gattcaggaa
     6541 cggaaccatc gacaacaacc atttcttcca gttcggaatc tgggacaagc agctcgacca
     6601 caagtacaga agaaacatca gaaacaagcg aatcaacaca aatgagcaga acaaattcct
     6661 cagaaaatta agttaaagga gtaattttac aactatgggt aaatattttg gaacagacgg
     6721 cgtacgcggt gaagccaaca aggaacttac gccagaactt gcatttaagc tgggacggtg
     6781 cggaggttat gtcctaagcc agcatgaaga agaaggaaaa cgcccacgcg tgttagtagg
     6841 gcgggataca agaatctcag gtcaaatgtt agaagaagcg ctgatttcag gacttttatc
     6901 ggtgggtatc gaagtgttcc agctaggtgt gatctctaca ccaggtgttg cttatctgac
     6961 acgtttgcaa aaagcaagcg caggggtcat gatttctgct tctcataatc cagcacaaga
     7021 taatggaatc aaatttttcg gtgcagatgg ttttaaacta gtagatgacc aagagctaga
     7081 aattgaagca ttattagatg ctgaagtgga tgaattgcct cgtccatcag ctgaaggatt
     7141 gggaactgta gaagaatttc cagaaggctt attgaaatat tctcaattcc ttgtacaaac
     7201 gatcaacggt gacctttccg gtttgaccat ttgtgtcgat gcagcaaacg gagcaacttc
     7261 aacaagtgtt aatcgtttgt ttgccgatct tgaaacagat ttttatacaa tgggcacaaa
     7321 tccagacggg ttaaatatca atgacggcgt aggctctacc catccagaac gtttagctga
     7381 gatggtcgtt gaaaaaggag cagatgctgg actagctttc gatggcgatg gcgatcgtat
     7441 tattgcggtg gatgaacttg gaaacattgt cgacggtgat aaaatcatgt ttatctgtgc
     7501 taaatacttg gcagaaaaaa atcgattgaa aaaagatacg atcgttacga ctgtcatgag
     7561 taacttagga ttccacaaag cggtggaagg aatcggtttg aaagatgtcg ttacacaagt
     7621 tggtgatcgt tacgttgtag aagaaatgcg caaaaacgat tataactttg gcggcgagca
     7681 gtctgggcac atgattttct tagattttaa cacaactgga gatgggatgc tttcaggaat
     7741 tcagttactg aatatcatga aacagacagg taagaaatta tcagagcttg cagcagaggt
     7801 aacaatctat ccgcaaaaat tagtgaatat ccgcgtaagt gataaaaacg gtgcgatgga
     7861 tgttccggca atcaaaaaag tgattgaaga ggcagaagct gaaatgggat cagaaggaag
     7921 aatcctagtt cgtccttccg gtacagaacc attacttcga gtgatggcag aagcgcctac
     7981 tgatgagaaa gttaattact atgtcgataa aatcgcggca gtagtcaaag aagaaatcgg
     8041 actggattaa gagatatttt cataaaaaat aaaagaacaa ggaccagtgt cagatgtctg
     8101 acactggtcc ttgttctttt attattctac tgtcactgat ttcgctaaat tacgtggttt
     8161 gtcaacgtcg aatccccgta atagcgttgc gtaatatgca agtaattgag tcggtacgac
     8221 tgtggttaga gtagttagat aagggtggac agttggtagc accagctggt cactttcctt
     8281 ttctaatccc tctgagacaa taactagtgt tttagcgcca cggctttcga cttctttcag
     8341 gtttcctcga gtatgcgcac cagtcacttc ttcactgatg attccgatca ctggtgttcc
     8401 ttcttcgatc aaagcaatcg ttccatgttt caattcgcca gcggcaaaac cttctgcctg
     8461 gatataactg atttctttca atttcaaagc agcttcaaga gaaacgttgt agtcagcacc
     8521 acgtccaatg taaaaggcat ttcttgtatc gctgagatat acttctgcca actcttctaa
     8581 ataatctttt tgggcgatga ccgattccat cgcattagca actaaactca attcatgagt
     8641 gatatcgaat acaagagaag tttcaaattc ttttttatca ccgatagctt tggctaataa
     8701 agttagtaca gctatttgtg cagtataagc tttcgttgat gctactgcga tttcgggtcc
     8761 agcatgcaat agcagtgtaa aggatgcttc ccgcgaaaga gtagaaccag caacatttgt
     8821 aatcgtaagt gaagggtagc cgagacggtt gatttttacc agcacttggc ggctgtctgc
     8881 tgtttcgcca ctttgactca agaaaataaa gaatggtttt tttgaaagaa gaggtgtgtt
     8941 atagccaaat tcactggcta aatgaacctc aacaggaatc tgtgtcaatt tttccaaagt
     9001 ctgtttacct gctaaccccg catggtaact agttccgcaa gcaacgatat agattcgatc
     9061 gctatcagac aatgtatcga ccagcttttg atcaagagag acacgattgt cttctgttac
     9121 gtatttttga gcgatacgac gcatgataat aggctgttcg tcgatttctt tcagcatata
     9181 aaaaggatag gtacctttct cgatatctga agcatctact tgtgcttcga atgaagaacg
     9241 ttctacgatg tcacctgcaa aattttgaat aataactttt tctgatgtga cggtaaccat
     9301 ctcttcatct tcaatctcaa cgaattcctt cgtatgagca agcatagcca ttgcatcgct
     9361 ggcaatcaca ttgaatccat cgccgagtcc gattaatagt ggacttttat ttttagcgac
     9421 gtaaataaca tcaggtgctg tccgatcaat caaagcgaaa gcgtaagaac ctttgatgac
     9481 ccgcaaagct tttaaaaacg cctcttgcgt tgtagtatcc ttcgcgaaag tttcaattag
     9541 atgagcgata atctcagtat cggtctcacc aataaagtgg tcatttgcta aataagcaat
     9601 tttaagttct tcaaagtttt cgattacgcc gttgtgaacg agcacgaagc gtccactttg
     9661 ggaagtatgt ggatgggcgt tttcttcaga aggttgcccg tgagttgccc atcgtgtatg
     9721 accaatacct atatttcctg tagtatcaat atttattttg tttttcaaat tttgaattct
     9781 gccttgtgct ttgaccagat aatcagttgt gccatctgag acaaagatac cagctgaatc
     9841 ataaccacgg tattctaatt tttctagacc gtcaatcaat cctggtgtta cattttttaa
     9901 tccaatcatt ccaacaattc cgcacataaa aaacatccta ctttctataa tgaattcgta
     9961 tgaatgcttc cagctgcagg aaaatccttt tgttttatct ttgcagacat tttttgtgga
    10021 ttttctgtag agtgcagtcc ctagggccat ccgccgaatc tttcgataaa ccctctcctc
    10081 gtcatctgtt tacacagacc tggcgctatc ctaaaaggta taactatcct cctttttgtt
    10141 ttttatctgg aaacatttaa aatcataacc ttttgcgaat gttcttgtca acaaaaaaag
    10201 aataaaaaat aaattggtat atatcaaatt ctttgtcaaa tgtccttttt atgtcaaaaa
    10261 ataatgggtt tttatctatc atctttttca ggagttcggt acaatagaaa gtaatcggat
    10321 agaggaggag taaaaatgga ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa
    10381 caattgtcca aattcgaagc atcagctggt gtatcagatg atacgaaaat atttttagat
    10441 actggctggg atagcatcca agaaatcgca cctgatgcct tggaagttgc tcaagctcgt
    10501 gagtttacgg tagaagatga attaacaaaa gagtcgtttt ccgggtatgc gcgggaagaa
    10561 aaagcagagc ggttcgacac ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg
    10621 tattagaaag gagaaagaat gaataaaaga cgttgggcag cagttctgat tgctgcagga
    10681 ttatttgtgg tttcgttagt ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt
    10741 gagggattga acagttggtt atatggggat gaagaactag ctccaaatgt gttggaagag
    10801 ggagacagta cgcaaaaaat cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg
    10861 tcttcaggtt tatttgcttc tgaaggttac aatcataaaa attttatgga acaactgaag
    10921 gcgattgaag aagatccaac agtcaaaggt atctttttag aagtaaattc tcctggtggc
    10981 ggtgtctacg aaagtgcgga aatcgctaag aaattggata cgattcgtaa agaacatgat
    11041 atcccaatgt atgtcagcat gaaaaacatg gcagctagcg gtggctacta tatttctgct
    11101 caagcagata aaatctttgc aacagaagaa acagtgaccg gctcaattgg tgtgatcatg
    11161 tcaggattga attattcagg acttctagaa aaactgggtg tggaagatac aacagttaaa
    11221 agcggagcgt tgaaagatat gggttcagga acgcgaccag agacaaaaga agaagaagcg
    11281 gtgcttcaag catatatcga cagcgcatat agtcgttttg tagcaatcgt cagcgaggga
    11341 cggaataaat ctgaagaaga agtcaaaaaa attgctgatg gaaggattta tgatggtgtc
    11401 caagcaaaag aagtaggttt agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct
    11461 atacgaaagg aacaacagct tgaagatgcg gaactagtag aatattcttc cacgccatct
    11521 gggtttgcta gtacatggtt aggaagtaaa ctagcagaat tccagggatt gaagccttct
    11581 gagactagtc agatcttatc cgttcttgag aatctaggga cagcggaatc accaaaagca
    11641 atgtattatt acggaggtga gtaagcatgt cagagctttc aaatgaaaat aaggaacaag
    11701 aaaaagataa gcaacagcag cctgctgtag gacctgactc caacttttcc caaaaggtac
    11761 tgagctcttt tgaaagagag cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat
    11821 ggaaaaagta tacggacaca agaaatgaac cgccaaaagc aaatgatttt cctaagtatt
    11881 tctatgcagg tttttttatc cgaatgtttg ccttcttggt cgatcttttg tgtatcggag
    11941 ctattacgcg tttaacactt ggtattgcca aaaatctagg ttggcttaat ttttcagatt
    12001 cttatctgag tctctacgga ttcacagcct tacttattta tctagcgtat tttattttat
    12061 tgacgaaatt caatcatggc caaacgattg ggaaaatgat ctttggtatt cgagtcatca
    12121 gctttgatga agaagaactt agctggtcaa ccgttttagt tagagaaggt gcttgccggt
    12181 tcattctaaa atttcctttg ctgatgttag ggtatcttcc agcagtcttt aatcagaaaa
    12241 agcaacatct gggcgatttt ttcactaata caagtgttgt tacgatcaat ctgatcaaag
    12301 cgttcaacca ggaagtcaac agctgatgag caataaaaag cagtaacagt tgaaagcacc
    12361 ttctaaaagt cagatctaaa atctagcttt tagagggtgc ttcagttatt tttaattgtc
    12421 atgctttttt tgttttgcta ctattgaaca tgagctgaat atgagcaaga ggaggacact
    12481 gcgtgaagcc gattgatttt ccagaaaaat atgagaattt aatgcgagtg gcacaacaag
    12541 cactagcaaa ccagcaatat cagcaggcaa aagaattatt tcaacgagct tacgagttga
    12601 aagaaagctt tgaggctaat agcctgcttg ttttttgtct ttacgagttg gacgagaaaa
    12661 aagaagcatt gaagcaagca ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg
    12721 ctgaatttta ttttgacttg ttaatcagta cacaagattt tttatatgct agaaaattga
    12781 tagcaagtac agatttttat gaagctttcg agcagcgggt cattgaaaaa atccaatttg
    12841 cagaagaatt atccggacaa atggagaggc agaagatcaa ggcactccac aataaatcgg
    12901 aggaacttcc ttcgttggaa cctgcacgtc agcttgcgtc tattgatgag atcgaacagc
    12961 ttccttatca tgagtttatc caaacagcga gtaaacttat tgtatcgcca gaggtccata
    13021 ttttagcaag ggcaaaatta ctggagacac ttaggcaatt gaacgaatgc catcctgttt
    13081 tctatttgac gattgaagaa aagttagtca aagtcatacc aaaagatctg ccaaagccgc
    13141 agcagcaggc aagttaccag caattatgtg tattttcaga tcgttacggg aatgaagatg
    13201 cgctgcttag ttccgtgtta aaagaagaat tcacgttgca atctgcaatt gtttatcctg
    13261 tctatgatac atacatcgaa gacccgaagc gctggtttca actgactgta gaggcgtgta
    13321 caggaaaaac gatgtatgaa ggtacagaag atgaaaaaca agatttttta aaaaaaagag
    13381 aaaaaatttt gcagcaaatg atcttcttcc attagatttt atcagtgaaa aagataaaaa
    13441 tgttatgaaa aacaaggatt ctatccattc aatatgttta caatttgaaa gggttagtgt
    13501 actatttaaa ggtatgcatg aaacgttttt gatgtataaa atgaatttct aaactcggag
    13561 ggaattaaat gactgcaaca tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta
    13621 cgattgcgca agacgaaatc caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa
    13681 gcttgaacgt acctggattc cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt
    13741 acggtgaaga agctttatat gaagatgcat tgaatgctgt attaccagaa gcttatgaag
    13801 ctgctgtcaa agaagctggc atcgatcctg tttctcaacc aaaaatcgat gttgaaagca
    13861 tggaaaaagg tcaagactgg gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat
    13921 taggacaata taaagatcta gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg
    13981 tggatgcacg tatcaaacgt gaacaagaag cacaagctga attagttatc aaagaagatg
    14041 ctgcagctga aaatggcgat acagtagtaa tcgacttcga aggctttgta gatggcgaag
    14101 cgtttgatgg cggaaaaggc gaaaattact cacttgaatt aggttcaggc tcattcattc
    14161 caggttttga agatcaatta gttggtcata aatcaggtga aagtctagat gtaaatgtaa
    14221 ctttcccaga agattatcaa gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga
    14281 caatccatga agtaaaagca aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg
    14341 ttgatgacga agtatcaaca ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag
    14401 aaacaaaaga aaaagcagcg gacgatgcaa aagatgaagc agcgatccgt atggctgttg
    14461 aaaacgcaga aatcgttgaa ttgccacacg tgatggttca tgatgaagta catcgttcaa
    14521 tggacgaatt cttgaacaac atgcaacgtc aaggtatctc tcctgaaatg tactatcaat
    14581 taactggttc tacagaagaa gacttgcata aacaattcga aggtgaagca gaaatgcgca
    14641 caagaacaaa tcttgtgatc gaagcaattg cagctgctga aaatcttcaa gcaacagaag
    14701 aagaaatcaa tgcagaaatc caagaattat cagaaacata caacatgcca atcgaacaaa
    14761 tcaaacgcgt gttgacagaa gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg
    14821 aattgatcac aggtacagcg acagaagcga aagaatagaa atcaagtaat aaatcatttc
    14881 tgaagaagct acttctagaa aattgtttat tcgaaataag ggactgtgac acgattttgt
    14941 cacagttcct tattttttgt taagagtaaa aaatgcaaag gtataggtca gttgatcctt
    15001 tgttaaacac tttttctttt tactaaacta tgttatgata gtaaaagact gtaaagtctt
    15061 tgatagtttc ttgcatactt tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa
    15121 gtagcaatga gaccgtacgc tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga
    15181 tcgtagctgg tccaggggtc tacatctgta atgaatgtat cgacctatgt aaagaaatca
    15241 ttgatgaaga attttatgaa gaagctgtac gcgaattcac tgaagtccct aaaccactag
    15301 aaattcttga agtatttaat aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag
    15361 ctgttgccgt atacaatcac tataaacgtg tcaatcagca agcacaagaa aacacagatg
    15421 atgttgaact gcaaaaaagc aatatctgct tgatcggacc tacagggtct ggtaaaacat
    15481 ttttagcaca aacattagca aaaacattga atgtaccttt tgcaatcgca gatgcgacaa
    15541 gtttgacaga agctggttat gtgggggaag atgtagaaaa cattttgctt aaattgcttc
    15601 aatctgctga ttacaatgtg gaacttgcag aaaaagggat catctatatt gatgagatcg
    15661 ataaaattgc gcgtaaaagc gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg
    15721 tccaacaagc cttgctgaaa attctagaag gaactgttgc aagtgtgcca cctcagggcg
    15781 gacgtaaaca tccacatcaa gaatttattc agattgatac gacgaacata ttgttcatcg
    15841 taggtggtgc atttgatggc atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga
    15901 ttggatttgg tacacaaaac caaaaacttt ctgaagacga aagtgtcatg cagcatatca
    15961 ttcctgaaga tttactaaaa ttcggattga tccctgagtt tatcggacga ttacctgtta
    16021 tggcagcttt agagaaattg acaacagatg atctggtacg gatcttgact gagccgaaaa
    16081 atgcgctagt caaacaatat caaaaacttc tttctctaga taataccgaa cttgaattcg
    16141 aacctgaagc gttgagagcc attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag
    16201 gacttcgttc catcattgaa gaaatcatga tggatgtgat gtttgatatc ccttcaaatg
    16261 aaaacatcga aaaagtgatt atcacaaaag aagcagctga attgtctggt gagccaacag
    16321 taatctataa agattcagag aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca
    16381 ttttggcttt tgcgacagcc gcttgcttta ttcatcacct tgttaaggag agagaccatg
    16441 aaagtccatc aagctgaaat tatcataagt gcagtggcac caaaacaata tccagataca
    16501 caattgccag aaatagctct ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac
    16561 acattgattg atcgaaagaa tttagcccgt acttccggta agccaggaaa gacccagaca
    16621 ttgaattttt atttgatcga gaacgcatta cattttgtcg atgttccggg atatgggtat
    16681 gcaaaagtat ccaaaactga gcgagcaaaa tggggaaaga tgatcgagac atatttaacg
    16741 actagagacc agctacgtgc ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa
    16801 gaagatatcc agatgtatca atttttgaag tattatgata tccctgtaat tgttgttgcc
    16861 actaaggcag ataagatccc tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa
    16921 gcgttggatt ttgatcgcaa tgacacgttt attctctttt cttccgttac aaaacaggga
    16981 aaagaagaag cgtgggcagc aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga
    17041 cttaccagtc aaactctttc ataaaagttt ggctggtaag tctttttttg agcttaactt
    17101 acttttgttt cttgtctttt ttcttttcat tttgtggctg tggttttaga aatcgatcga
    17161 tctcttcttt tggtgcaact gttttttccg gatcgacagc aaatgattcg aaaagtttat
    17221 ctaaattatc tgtacgttca aatttcaacc ccattgtaaa cacctctttg tttcttttta
    17281 cttagtctat catgtaatcg tccaaattgg tttctaagac ataaagtttt agtttttctt
    17341 tatcattggt tataattaaa ttataaaaag atttgaaaga ggtgagaaaa tgattggcga
    17401 agataaatta ttgatgacta tccagctcac agacttaaat gaagaagaaa aaaaacagga
    17461 aataagcgat tgggctattt tgacacgtct gttgatccag ccaacattta tccgctcttt
    17521 cacacaacaa gcggatccgt atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc
    17581 ggtaagagac gaaagttggt tagttgtagg aaatgacgag aacgaatgta gttttcgctt
    17641 ggaagataat caattattga taaaaaatgt attgtctatc tctgttttca aagaaaagca
    17701 atttttgatt cgagacttca ttcagaaaaa aatgattgct catggggtgt tcgcctatat
    17761 gcgagcttat agcgagttta tctatcacaa tacaaaacag atctctcaac ggctattgtt
    17821 tgagaaaaag gatgaaatcg aacatttacc taaaatgaaa caacaaaacg gggaagtagt
    17881 agtcgattgc aaccaatttc caggatacga tcttttttac caaggtttat gtttcacttc
    17941 gtgttgggaa atgtattata gtcgctatta tcatcaaatc atccctaaac cgatttttct
    18001 cgacatccaa caagtagaaa aagtgaagga attagaaaac gaagtcatat gtatccagct
    18061 ttatcgagac ccctttaact ggcaaaagcc aaacaatcaa atgtttcaat cctattttag
    18121 ggaccagctt gggtttgatc atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc
    18181 attcgttgaa tacatttaca ctgaccatac gatccaatct gtccagtatc agaatgcaca
    18241 aatgcaacct gttcctaaaa agaatgcttc attttttgta acaaaaagtt atgatatcca
    18301 acaaggagat tataaagaaa ggcgtgttcg cggaacacta aatgctcagg cgtattttcc
    18361 ttgggttgac gagaatcgat cacgcatgat gtgctataaa gttatcgatc cgactgttgc
    18421 tttggataat ggaatcgaag cttattgtta ttatattagg gaatatctag aagtagaggt
    18481 aacagatgaa aaatatcaag agtatctttt atccttaaga atctatgttc cttcagaatc
    18541 cctgtcagaa ttaccgttaa aggaaatcaa acatagactc tctgatgtta ccttcaaacg
    18601 aataaagaaa aagcggcgaa gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg
    18661 tgtgcaattt ttagattacc gagaattaga acaattgatt actttacaaa aaatatgagg
    18721 tgaagaaaat ggataaacaa ctctattatc gaataatggt tccggcatca ttattatccg
    18781 tctgtctctt ttttgttcct aattttccta ttcgatcagt catcggtttt ttggtcatgt
    18841 ttggcgggat gggactttat tattatcttc ttcataagga aagaaaacct gcttctgttt
    18901 atatgaaaaa aacaagaagc gaaaataaaa aacgataaaa cggcttgatg atagacaaaa
    18961 aaataaaaaa ccagtctttc t
//
LOCUS       DARBEG010000052        18740 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000052 DARBEG010000000
VERSION     DARBEG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.052"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            256..531
                     /locus_tag="SCK20_001791"
     CDS             256..531
                     /locus_tag="SCK20_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145840.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="SCK20_001792"
     CDS             544..2616
                     /locus_tag="SCK20_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG1145841.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="SCK20_001793"
     CDS             2781..4667
                     /locus_tag="SCK20_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1145842.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="SCK20_001794"
     CDS             4679..5626
                     /locus_tag="SCK20_001794"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG1145843.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="SCK20_001795"
     CDS             5646..6173
                     /locus_tag="SCK20_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1145844.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="SCK20_001796"
     CDS             complement(6236..6889)
                     /locus_tag="SCK20_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG1145845.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="SCK20_001797"
     CDS             7021..7863
                     /locus_tag="SCK20_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1145846.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="SCK20_001798"
     CDS             8021..8917
                     /locus_tag="SCK20_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1145847.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="SCK20_001799"
     CDS             8920..9633
                     /locus_tag="SCK20_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG1145848.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="SCK20_001800"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="SCK20_001800"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1145849.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="SCK20_001801"
     CDS             10168..10395
                     /locus_tag="SCK20_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG1145850.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="SCK20_001802"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="SCK20_001802"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1145851.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11681)
                     /locus_tag="SCK20_001803"
     CDS             complement(11181..11681)
                     /locus_tag="SCK20_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1145852.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="SCK20_001804"
     CDS             complement(11960..12358)
                     /locus_tag="SCK20_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1145853.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="SCK20_001805"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="SCK20_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG1145854.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="SCK20_001806"
     CDS             13471..14238
                     /locus_tag="SCK20_001806"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG1145855.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="SCK20_001807"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="SCK20_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG1145856.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="SCK20_001808"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="SCK20_001808"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG1145857.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="SCK20_001809"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="SCK20_001809"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG1145858.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DARBEG010000053        18581 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000053 DARBEG010000000
VERSION     DARBEG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.053"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(98..922)
                     /locus_tag="SCK20_001810"
     CDS             complement(98..922)
                     /locus_tag="SCK20_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1145859.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(895..1575)
                     /locus_tag="SCK20_001811"
     CDS             complement(895..1575)
                     /locus_tag="SCK20_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1145860.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1598..2536)
                     /locus_tag="SCK20_001812"
     CDS             complement(1598..2536)
                     /locus_tag="SCK20_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG1145861.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2781..3644)
                     /locus_tag="SCK20_001813"
     CDS             complement(2781..3644)
                     /locus_tag="SCK20_001813"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG1145862.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3786..6359)
                     /gene="mprF"
                     /locus_tag="SCK20_001814"
     CDS             complement(3786..6359)
                     /gene="mprF"
                     /locus_tag="SCK20_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1145863.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6500..7306
                     /gene="proC"
                     /locus_tag="SCK20_001815"
     CDS             6500..7306
                     /gene="proC"
                     /locus_tag="SCK20_001815"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG1145864.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7385..8941)
                     /gene="rny"
                     /locus_tag="SCK20_001816"
     CDS             complement(7385..8941)
                     /gene="rny"
                     /locus_tag="SCK20_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG1145865.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9352..10401)
                     /gene="recA"
                     /locus_tag="SCK20_001817"
     CDS             complement(9352..10401)
                     /gene="recA"
                     /locus_tag="SCK20_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG1145866.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10510..11751)
                     /locus_tag="SCK20_001818"
     CDS             complement(10510..11751)
                     /locus_tag="SCK20_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG1145867.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11991..12569)
                     /gene="pgsA"
                     /locus_tag="SCK20_001819"
     CDS             complement(11991..12569)
                     /gene="pgsA"
                     /locus_tag="SCK20_001819"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG1145868.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12778..13701)
                     /locus_tag="SCK20_001820"
     CDS             complement(12778..13701)
                     /locus_tag="SCK20_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145869.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14440..15726)
                     /locus_tag="SCK20_001821"
     CDS             complement(14440..15726)
                     /locus_tag="SCK20_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1145870.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15719..16987)
                     /locus_tag="SCK20_001822"
     CDS             complement(15719..16987)
                     /locus_tag="SCK20_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1145871.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17239..17700
                     /gene="mscL"
                     /locus_tag="SCK20_001823"
     CDS             17239..17700
                     /gene="mscL"
                     /locus_tag="SCK20_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG1145872.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17760..18206)
                     /locus_tag="SCK20_001824"
     CDS             complement(17760..18206)
                     /locus_tag="SCK20_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145873.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18299..18544)
                     /locus_tag="SCK20_001825"
     CDS             complement(18299..18544)
                     /locus_tag="SCK20_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG1145874.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT         5315 a         4052 c         3240 g         5974 t
ORIGIN      
        1 ttttatgaaa aaataagaaa atagttcggt atttattaaa ataaaaagaa gcctataaaa
       61 aatttttcgt ttttataagg cttcttttct ttataattta gttatttttt cttctgaagg
      121 caaccaatat tcgtttgatg atatttacaa tcaggaacaa agcaataaag actaatgtga
      181 ttgttgctcc tggaggtgta tctagataaa acgagctagt tagtcctgac agcatcccta
      241 gaaatcctac acaaacactg atagcaatca cgacattgaa gcttttgcct aatcgcatac
      301 cgattgctgc gggaagaacc atgattgcgg atatcaaaag tgcaccggca atcgggatca
      361 tcacagcaat agccacacca gtaatcacat tgaacatcat cgacatcaaa tgaacgggta
      421 gcccatccac gtgggcggta tcttcatcaa aagtcagaac atacatcggc cgtttgaata
      481 aaaagaacaa tataccgatg gcaacgaata agattcctaa aaagatgact tgctcccatg
      541 taatggtcac gatcgaacca aacagatagg attggatact tgttgctgaa ttccctccgc
      601 tcaaattcat caatactaac gctagcgcca aaccaccagc cattaaaatg gcaatggaga
      661 tttctgaata ggttcggtag atcgttcgca gatactccaa tatcacagct gctaatacaa
      721 cgactagcat ggtcgtaagt gtaggattaa ggttcaaaaa gaatcctaac gctacccctg
      781 ctaaagagac atgagaaagc gtatcagcca tcagcgattg tcttctgatc actaaaaaca
      841 cacctagcat cggggcgatt ccggcaatga agatagcggc aagaaacgct cttctcatga
      901 attcatatga aagcaacgcc attgagaatc ctcctttctc accagtcgga tctgtctgtc
      961 cgcatactgt ttgatatctt catgatcatg ggtgatcatt agaactgatt tcccatggac
     1021 atgcgcacta tgtcggagaa gacgataaaa ttcatttctg gattcttcat ccatacctgt
     1081 tgttggttca tcaagaacaa ataagtcagg gtcagtagca aaaatacgcg ctaaactgat
     1141 tcgctgcttt tgtccgccag acaattcacc tattcgccga tttcgcatat cccacatccc
     1201 tacagcttct aaagcacgct gtacatgttg atgatctttt tcagagagac gcttgaacca
     1261 gcggcctctt ggatatcttc ctgaacggac taattcgatc accgtactag gaaaacctgc
     1321 attgaaagat gcaacttgtt gaggaatata accgatactt aatttttttc cttcttgatt
     1381 tttctcagca atctttatca ttccttttga aggtttcagt aatcctaacg atgctttcac
     1441 caaggttgac ttggctgctc cattttctcc agttaaaatg acaaattcac ccgcatctac
     1501 atgataagag acatcttcta gtacaggttc atcatcatag taaaaggtca aatcagatat
     1561 ttcgatataa cgcaccttac aacctccatt ctaaaactta attgatactt tcttttaaag
     1621 ctttcagatt tttttccatg acagaaatat aattttcgcc tgctttgatt tgttcattag
     1681 tcaagctttc taaaggattc aacacttcta gcttcactcc tgtttcattc gacaatgtct
     1741 cagctacctt cgaagaggca ttttcttcaa aataaatcac tttgatgttg tttttcttca
     1801 caaattcttt tagctcagct aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg
     1861 aaatcgctac ttgttttaag tcgtattgat tcgctaaata agcaaaagca gcatgctgag
     1921 tgacaaatgt gcggtttgtt gcatctttta gctcatcgga gtatttttga tccagtgctt
     1981 caagcttttt gatataagca tcggcattct tcgtaaaagt ttccttttta tctggatatt
     2041 tttcgcctaa ttcttcagca atcgttctga cttcttttat agctaatacc ggatcgagcc
     2101 aaacatgtgg atcgtattca tgtgaatgac catgcccttc ttctgattct tcatgttcgt
     2161 ctgcttcttg cgattctaaa cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt
     2221 tgctatccac tgaatccttt aacgaatcca caaaaaattc catgtctgaa ctattataca
     2281 cgaaaacatc tgcatcggaa atttctgctc tttcttttgc acttggttcg aaatcatgcg
     2341 gctccgttcc agcagggatc aaaagtttca cgtttccttc atcccctacg atctcttttg
     2401 taaaatcata catcggataa aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt
     2461 ccttgttcgt atttccacat gctgcaaaca gtaaagcacc tatcattacc gtagctgtca
     2521 aaagatattt tttcatttat atgctccttt caatgaacaa atcggaattt tacgatttat
     2581 taacgtgaac taatgtatca aaataaaaaa acacagtcaa tagaaatcca tcaatttctg
     2641 atttaagcat gtatattctt gattaaatcg taaaatggat gagttgctgc tcatatctag
     2701 ccatttcact acaataaaaa actgaagaat accattttag gattcctcag tttttcatca
     2761 cctattttac ttcttatatt ttattgcagg gttcgtacta aataaacttc ctcacaaaaa
     2821 ccttttaacc cattatatac tctctgcgct cttgaataat gttgacacaa ggcaaaaacg
     2881 gttggtccgc ttccactcat caatgcagca tctgctccgt acttgatcat acgctctttg
     2941 atttgatgga tgactggatg ccgctttccc gttatatctt ctaaactgtt tcccattttt
     3001 ttgatcatct gctgataatc ttgctctcgg atggcttcag caagtccgtg gatgtcttga
     3061 tgttggatgg cttgcgtatc tacttctcta aacactgtac gtgtagagac actcattctt
     3121 ggtttgacca acacaaccca gcactgtggc atagaaggta atacttggat ttcttcccct
     3181 ttgccaccga caaatgccgt acttccgtag acacagtaag gaacatctgt accgacttcc
     3241 aaaccgattt ctgccagttc ttctaacgaa cagcccagat tccataattg gttgatccct
     3301 cttagagcag ctgcacagtc tgtacttcct ccgcctaatc cagctgctac tggaatattc
     3361 ttcttaacaa agatcttgac accggttttg atattgtatt tattttttag caataaagca
     3421 gcttgataaa cattgttccg ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg
     3481 attttgtctt ctgttatttc ttcaaaaaat agatgatctg ccaagtctac gctcgacatc
     3541 accatttcca actcatgata cccatcttgc cgtctatata atacatccag tcctaaattt
     3601 atcttggctg gcgctttttc aatcaattcc atcctctcac ccattcgaaa agtctaagtc
     3661 gcaagtattc tatcataggg aaaaataaaa gtatagctta tgtctgccta gtggttgatt
     3721 ttgtcatcgt ttcaacagct ttcacagaat tttctcttca taatccaaac aagttctagt
     3781 gattcttata attctgtgag cacttcttct gccaaactcg gaacatttcc tttttctttt
     3841 cctatcagac tgagtagctg catggtagca aatccgaaat tacttccttt cctataagca
     3901 aaataacgtc cttcccaatg atcgacatat tttaatttct tttcataagc agactggaag
     3961 gaatagaaaa aattaccata attatatacg atatgcatga ctttttcttg aaagaaagaa
     4021 aaatctgttt ctccaacatt agctaatgga gctaagccta gattggcttc cgagtagccc
     4081 attttctcgt atacaataaa cagattggct aacagatagt cagctaattc ctcagattct
     4141 ttaggtaaga cccgcaacaa atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg
     4201 atgaagccaa cgactttttg atctttacgg acaataccga caccgctaga taatacatat
     4261 gatttatcaa atcgtcccac agagaagtat ttttctcgtg caccttgaag ccatttttgt
     4321 gaaacttcat ttacagcttg atgtaattct tccggcagtt gatcgtacca agtaaaatgg
     4381 tatccttctt tcatcaaacg ttgatattca agttgctgac tcagccgaga caagtcttct
     4441 gttgctagat caacaatccc tgcttcacct actttcgtga agtgaaaacc caattcatga
     4501 agcatcacta cgtagctttc tgatatctta taaaacgcaa gctgatagcc taaacaatct
     4561 gcttgtttca taaactgatt cgttgctgcc cgcatcattt taggattgcc aactggatct
     4621 cctaaaacaa agcatttgtt tcctttgatc tgataaccaa aaacgacttg atcttggtcg
     4681 tcttcttggt aataaaaata actgtaacct gacaagcgaa gctgatggct tgtagctgtg
     4741 cctccgtatg tttccaataa acggtcaaaa cggtcttttt cgtactcact gccaagcttc
     4801 gtagtatctt cagctaaata ttgataaagc gccactaaag ctagcaatga gatgcccaaa
     4861 ccaataagcc ctgagaacca cacatcatct gaaggaaaca aaataaaatg atttcctgtt
     4921 attcgaccga ctaaaaagcg tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa
     4981 aagccgaaca agctagcatc aaagattata gctccccaag aataaacgaa ttttgttcga
     5041 taaaattctt ttctggataa ccacacagct aacagaatca agacatatac caatatcaac
     5101 tgccaagata acgtacgtgt gattgtattt aaaataccaa aagatagtaa agcaatcgta
     5161 ggcagaaatg cctttttgac ttttactgaa agtgctctag caaggccgag taataaaaag
     5221 ccaaccaata gattcactgt ctgatctaaa aaattgaatg agaacggcaa taatacttga
     5281 aatagccgac tgattgcaga cagattagtg attgttgata gaagtaccat catgatcccc
     5341 gcaaaataga cagcacttac gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga
     5401 atgttatcta aaaatcgatt gattttgact cctgtatgtg tgatgaacaa tagaagtccc
     5461 gtcataaaag gtagaacata ataaaacaac cgataatata atagccaaac tactgctaca
     5521 tcttgccgaa cagacaattg actcattcct aagatcatca aaacgtcaaa agtccccatt
     5581 ccacctggga ccatcgtcag cattcctatc aatgtagcaa tcatgaacat aggatagatc
     5641 gaaagcatcg aaatatctat tctcatcaac ttgccgatga tcaaaaatac cgcaagggct
     5701 cccgtccatt gtccaaagga tgccagtagc agaccaagca agccttttgg ataaaactcg
     5761 aagaacaatg tctttctctt tagataagca aaaaagaaaa tgactggtgc taaaaaacta
     5821 ccgccgaaaa gccagatcca atagcttcga aaaatacttt ctgactgaat aaaaaagaca
     5881 tcaataaatg taagaaacga ccatacagac aaaccagtca gcataaatag tgcgactttt
     5941 gatacagtgg ctaaaaccat ttttctattt gtctgctttc cataaaaact agcccgcaga
     6001 cttgctccta ccacaccacc aaaaccggcg agattgttga tcgtgtttgt tacccacgca
     6061 gcaagaagaa actcttttct ttccattttt ggttttcctt gtttttctaa gactctgatc
     6121 gtcacccaat catacaacaa catcggagtg acacccagca gtccagccag aatcatgagc
     6181 aggatcgtca tgcggctttg ttgtcccatc ctttgccaaa tatcttgcca actcatgcca
     6241 ttagcaatat ttgcgacttg atttgccaca aagactaaca cggagccgaa aaaaatcagt
     6301 tttaaaatca gactatgttc ctttatccat tgcagcatca agtatcgttt atttttcaca
     6361 ttttcccatt cctaacttgt atacttttgt gtcatacccc aatgttatct tatttatttt
     6421 aatttttcca ctttagacct gttctttgct tgcgatgata gaaaaagaaa gatatcctaa
     6481 tgctaaagga gaggataaca tgaaagtagg atttatcggc acagggaata tggcacaagc
     6541 tattattgat ggtttattaa aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag
     6601 tggacatttt gaaaatgcac aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag
     6661 taatcaggaa gttgcatcac aatcagatat cgttctttta gccgtgaaac caaaaatcat
     6721 tgaaaaagta ttggaagaac taagagaata tagccagaaa gtcttattca tttctattgc
     6781 ttcaggaaaa tcattaatag atttagaaac agcgcttgaa gatcctcagg cctccatcgt
     6841 acgtgtgatg ccgaatgtca atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa
     6901 tgaagctgcc acggaggaac agcttcagca agctacgatg atttttgaag caatcggcag
     6961 cgtctatcct ttagcagaat cagatttcag tacattcatt ggccttgctg gcagctcacc
     7021 cgcattcgtt tacatgctga tcgatgcgat gggacggact ggtgtactga atggtttgtc
     7081 caaacagaca gccactgaga ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct
     7141 ttcaagtgat aaaaatccat gggaacttgt agaccaagtt tcttctcctg gcggtacaac
     7201 tgttgcaggt gtagttgcct tagaagaagc aggattcatc ccagcaatca tcaaaggaat
     7261 caatacgacg atcgagaaag atcttgagat gttgaataaa aaataatgat agcaatgttt
     7321 tttcgtgcct tacaaaaaaa gaagagctgt ggcttttgcc gcggctcttc ttttgtattt
     7381 tttattattt agcataatca gttgcacgag tttcccgaat aacgattact ttgatgtgtc
     7441 caggataatc cagttcttct tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt
     7501 ctaaatccga gatttcttct ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg
     7561 cgtaacttga ttctacaccg gcgaagctgt tcgaaatatt ttccagactt cttaaacgac
     7621 ggatgtagtt ttctaatgat tcacttcgcg caccaggtct tgctgctgag agcgcatctg
     7681 ctgcagctac aaggacagag ataacagaag tagcttccac atctccatga tgagaagcga
     7741 tcgcgttgat tactactggg ttttctttgt acttggcagc taattccgca ccgatctcaa
     7801 cgtgagatcc ttcaatctca tggtcaagtg ctttcccaat atcatggagc aacccggcac
     7861 gttttgctaa ttggatatct tcgccaagct ctgctgctaa gataccagct aattttgcaa
     7921 cctcgaccga atgcttcaag acgttttgtc cataactagt tctgaatcgc agacgtccga
     7981 tgatcttgat tagatctgga tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc
     8041 cgtattcgcg aatacgttca tccatttctt tacgagattt ctcgaccatt tcttcgatac
     8101 gcgctggatg gattcgtcca tcttgtatca gtttttccaa ggtcatccgt gcgatttctc
     8161 ttcggattgg atcgaatccg gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat
     8221 cgataccagt cagcgtctca agcgttcgaa tattacgacc ttctcgtcca atgatacgac
     8281 ctttcatttc atcattcgga agggtaacga ctgaaacagt cgtttctgaa acttgatctg
     8341 ctgcgcagcg ttgaatcgct aatgataata agtttttcgc tttgcgatct gcttcttctt
     8401 tggcacgctg ttcagattct tttaccatta gagttaactc atgattcagc tcttcttcag
     8461 tcgatttcat gatgacatct cgtgcgtcct cttttgataa accagcaatt ttttctagtt
     8521 ctttttgttg atcttcaatc attttttcaa cgtctttttc tcgctcttca attaattgct
     8581 gttttgaact aagtgcttct tctttacttt caagtgagtt ctcgcgtttt tctaacgagt
     8641 catctttacg attcagcgtt tgttcgcgtt gaatcaaacg attctcttga gattttaatt
     8701 ctaacttact ttctctcagc tcactttcaa cttcagaacg gtatttctga ttttcttcct
     8761 tagcttctaa caatgcttct ttttttagag tttctgcttc tttttttgca ctatcgatga
     8821 ttccagcagc tgatgattgt gcaccagcta tttctttctc gtgacgtgac tttgcgacca
     8881 taaaccctaa accaagaccg acaattaaac cgatgatagc gaggagaatg ttgaatacca
     8941 taaaatccac ctccatacta tctttttcat ttttatcatc tatacagctt ctatgataaa
     9001 ttattctaaa ctcgtcaata tgcatcttcg catatatgtg tacaaaaaac atgcacaact
     9061 ttattctaat gtttgtaccc cgtagtgtca actttaaata aacaaagaaa cggctttcta
     9121 cttattcatt aaacgatggt ttttaaaata aatgacacta gtttcatata ggtaaacctt
     9181 taaattgaga aacttttcta ttaaattatt ctttgtagat gaacagatat ttttatttta
     9241 tgtacccact cgtataatta aataactcac gatttcttta ttcaataaaa aaacggcagg
     9301 tcaaggagag tacctactct tcagaccagc cgtttttgtt tatcataatt tttattcttc
     9361 tactaaaggc aattcttctt gtcctttttc atcttctggg acagcgactt cttcaccaat
     9421 accataagct gctctaacta gagcagaaac ttctgccatc atttcaggat gattagccat
     9481 atagatttta gcgttttccc gtccttgacc aatccgatct tctttataag aataccaagc
     9541 accgctttta tcaacaatgt ctttttctac tgccatgtct agaagttctc cttcttgtga
     9601 gatccctaaa ccatacatga catctacttc agcgattttg aaaggaggtg ctactttgtt
     9661 ttttacgact ttgatctttg tacggttacc aacaatatct gtaccttgtt tcaattgttc
     9721 tgcacgacgt acttccaaac ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt
     9781 gatttctgga ttcccaaaca tcacgcctac tttttcacgg atttggttga tgaaaatagc
     9841 gattgttttt gttttgttga tcgaaccaga gagtttacgc aatgcttgag acatcaaacg
     9901 tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg atttctgctc gtggaactaa
     9961 agcagcaact gaatcgacta ctacgatatc tacagcccca cttgatacta aagcatcagc
    10021 gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac
    10081 acctaatttt tgtgcatatt gcggatctaa cgcatgctca gcatcaatga aagcggccgt
    10141 tccgccattt ttttgtactt ctgcaatagc gtgtagtgca actgttgttt tacctgaact
    10201 ttcaggtcca tatacttcga tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac
    10261 atctaacgca agagagccac ttggaattgt agagatttgt tggtcgattt tttcgcccaa
    10321 tttcataata gagcctttac catagctctt ttcaattttt ttcagtgcag catctaaggc
    10381 tgctttacga tcatctgcca atacgataat cctccttata tttacaaatc agagcaacat
    10441 ttcattatcc ttatcatatc ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat
    10501 tcgcatttat cattttctta aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc
    10561 attatatctg atagcagcac gatctctggc aaagtgatat ttttccgaaa ccacgccgtc
    10621 tttagatgaa atagcgatcc atactgttcc tgcagattga ccttctaacg tgtccggtcc
    10681 agccactccg gtgaaagcta cactgtaatc tgttcttgct atctcacgtg cttgaatggc
    10741 catctgctcc acacattctt tgctgactgt tccatattct gctaatagct ccggatcaat
    10801 ctccagaaaa ccagcttttg tttggagcga gtaggtaacg aatccgcctg gaaatacttc
    10861 agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg ccggctgtca aactttccgc
    10921 tgctgttaca gtctgcttgt tttctttcag tagttccact acgacttttg ctagcgaatt
    10981 atcatcgccg tatccataaa aatattcccc tactcgttct tggatttttt cttctaatgc
    11041 taaaagagct tgattccctg cctgaacatc atttgttttc actgtcagtc gtaatgtcac
    11101 ttcatttggt ttggcatatg gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt
    11161 tagctcagtg accagtcgcg attcaccaat cccataaaag cgcaaaactt tagaaattag
    11221 tttttcttca gaaggaaaac gttcctctag taaaggacgt acttgttcca caaacattgg
    11281 tttcaactca ttaggcggac cgggaagaag gatatacgca tgtgtatctg tttgataaaa
    11341 agtccctaaa gcaagccctg tacgatttgg tagaggaacc ccgccttcga taatctggga
    11401 ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt tcaaaatatg ccaataattt
    11461 tttgtatcct tccgtatttt gtatgagaga cttaccgagg tgagccgcag ttgcttcttt
    11521 tgtcaggtca tcttctgtcg gtcctaatcc gccacataag agtatgagtt cacttcttgt
    11581 ttcagctaga gcaatcaact cttctaaacg ctgctgatta tctcctacaa ctgtttgata
    11641 ataaacttcg atacctagat cagctagctg ttctgacaag aaagtggcat tcgtattcac
    11701 gacttgtcct agtaaaagtt ctgttccgac agctattatt tctgctttca ttacttcccg
    11761 ctcccttata tagtatacgc tgtattttta tattttttta tttaaaaaga gggcaaggag
    11821 ctgtgacaaa aatcttgtca cagctccttg ttccaaataa acagtgttct cctgccaaaa
    11881 cctcggtcat aagtcacaat atacataaaa catgaaaagc tgttttttga aattatccct
    11941 tgtgactcag agttagacgg caggctcaca acctctccac taaacgatac ttacattgat
    12001 cctttgaaca caccgctgtt tttgatgaag taatccacac ctgaatagat agtaaagatc
    12061 agacaagcat aaagcatgat ttgatctact ggaataccga tcaggctgaa tgggatatta
    12121 ttgataaaca gcaagataat agcaaccatt tgtgtagccg tcttgacttt tcccggccaa
    12181 gctgcagcca tgacttctcc atgttcgaca agtagtaaac gcaagcctgt gacggctaat
    12241 tcacgacaga caatgatcgc tactacccaa ccaggggcct ttccttggtc aaccaacatg
    12301 ataaaagcag tcataactag cattttatct gccaacgggt ccgcaaactt gccaaaatta
    12361 gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc agtctgtgat actagctaaa
    12421 gcaaaaataa ctgcacctac tagctgtgtg acagccagtg tggtgtctcc tacgttaaat
    12481 tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa tcgggatcat acaaattcta
    12541 agtacagtta atttatttgg taaattcaca ctatcgactc ctttcttccc tattctacgt
    12601 gactatcagc gaagtttcca ccgatattat ttacttttcc aaatgaattt attcaaagga
    12661 gtatttttat tgaaattgat ctttttgaat taacgaagaa ggaccgcgac acagatttgt
    12721 cacgatcctt cttttttata cctacaagta aacagatgcg taaactaatt tatacgatca
    12781 ttctgcgtat tgaagagtaa ggttcacatt ttttggactc tgattgtttt cccctggatt
    12841 aactggtacg gattctccat tcgctttgat cgttgcattg cttgctgcac caatcgtgat
    12901 ggtcgcattt gttgctgttt ccggtaacac ggtactctgt gtttcatttg ctgccagtgt
    12961 cccttgataa acataggcat tgtctactag cacacctaac catacacgat tgacagcagt
    13021 aaattcaagg gttacaggct tggtagcgtc tgtgatattt actgcgatgg cactgccaga
    13081 atcttctcct gtagctattg ccattttctt ttcttcactt gattttgttg ttgattctac
    13141 tgtagaagac tctttggtct gcgtgctttg ctctgaggaa gaggactcct cgactgatcc
    13201 ttctacttta agcgaagaag ctgtgtctcc gattattgga tcagcttgac gatcttgcca
    13261 agtcatatag cccacgacaa cgacaatcgc caaagcaacc aatcctaata aaatgacagg
    13321 aagacttgtc caaaagcgac ttggatgcga ttcttcctga tgcattgctt ttctagaagt
    13381 acttactgtt tcaggctgcg gacgttcagg gaccgatcct gtcaatttgc ttttcccatc
    13441 aaaaacatcg actaaatggt ctccatcttc tcctacagct tcagcgtatt gccgaataaa
    13501 tgcccgcacg taaaatttac caggcaattg gtcaaaatca tcattgtcga ttgcttccaa
    13561 ataacgtttt tgaatttttg taatctgctg taattcatct aaagatattt ttttattcag
    13621 gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc actcgttcat cttctccagt
    13681 ctgtttgatt ctccgaagca tctgctgttg caacgaaaga cgttatgttt gggtgctgtg
    13741 acaaaagtca tgtcacagct ccttttttcc gaataaacat tgttctcctg ccaactcctc
    13801 ggtcacaagt cacaatatcc aataaatata agaagctgtt ttttgaactt gttccttgtg
    13861 actcggagtt aaacggcagg ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc
    13921 ctcggtatta gctcaaaaac cggcaaaata taaggagata tttttccgta tgtttctcta
    13981 atactcaggt caaaacgctg atttcacaac ctcttcctta aacggtgttc aaaagcttgc
    14041 atctgcggta ataagtccaa ccaagttaaa aacattaaga agctgttttt acttggctgt
    14101 tcttattact tgatgcaggg cagctttttc acaacctctt ggtacacggt gttccagaag
    14161 tagcttcttc ggcaataagc cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt
    14221 ccttcttatt tcttgaagct gaccacttct gtcacaacct ctttgattac ttcgtttgat
    14281 tttattttaa tataagttga ttaaaagaga gcatgatttg ttttattttc tcactaattt
    14341 ttccaacaca actaagaata ccataaaagc tgattttgga ggaggttcct actacaagtc
    14401 taaaacgaaa tttaagactt ttcctcataa aatactggtt tattttggtc tcatgtaaaa
    14461 ctcgctgaag gcctcttcac ttataaatgc ttccccagct gccagtacgt ctttcatttg
    14521 gatcgaatcg atgatttcag gaagatcaaa taaagttcga tcgccaaata aactttgcgt
    14581 aaattgattg gcaatatatt cgatcgaatt taatgattgg aaatattgtc caagcatttt
    14641 cttcttcagc aaatcgagat ttgtttctga cacttccgga tcatcagcga atccaagaat
    14701 gatttctttg actttttctg ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc
    14761 aaagtgaaat tcacgatcca agctgaattc aaaaccaaag ctgtcatcga taattccttg
    14821 attgtacatc gctagatagt ttcgagaagt attgcctaaa atcatttgga acaacagatt
    14881 gattgctgtt ttatagcgta acagttctgt tccttcttgc ggcaatgtgt ctaatccttt
    14941 aataccaagt acaaatttat ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat
    15001 ttcttttgtg ttttcaggga aataacgtac aatttcttgc tttggaggga aattttttgc
    15061 ttcttggttt tcccgaataa gtttcatcaa tttctcaggc tccatcttac caactacaaa
    15121 taaaaccata ttgcttggct gataaaacgt ccgatagcac gtatacaagt cttgagctgt
    15181 gattttgtcg atactttcaa ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata
    15241 caagttattt agtatgccaa aaaacatccg ccagttaggg tcgtcttcat acatttggat
    15301 ttcctgaccg atgattcctt tttccttgtt tacagtttct tctgtgaagt aaggtgcttg
    15361 tacaaaatcg atcagagtcg tcagattctt ttctacctga tccgtcgtag aaaacagata
    15421 gctggttttc gtaaaacttg tgaaagcatt ggcggaagct ccttgtttcc caaataattg
    15481 aaagacatca ccatcttctt tttcaaacaa cttatgttct aaaaaatggg caattccatc
    15541 tggaactttc accttttctt tttcaccata gggaataaat tcgttatcga tcgaaccata
    15601 attcgtactg aacaaaccat atgtcttgtg atagtcattt ttaggcaaca aatacacggt
    15661 caaaccgttt ggcagtactt catgataaag tgtttcattg atttgctcat attctgtttt
    15721 attcatttgc atttcctcct gctaaaaaga agattgcctg caattcgatt tgctcagcta
    15781 cttgttgtat ctctttgatc gttacgctct ccatacgttt caaccattct tcatcagata
    15841 attttgtttc cggcagccag ctgtccaaaa aagaagcttc gattgccgct tgcggagagt
    15901 ctagagaaag cagatattgg tttctcagca ttgcttttgt ctgagacagt tccaactctg
    15961 ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat cagatgaagt acttgatttc
    16021 ggttcttttc atcgataccg gtttggacac tcatgaatcc tcggaacgta tccacgctac
    16081 tagaggcata ataagctaga ctttctttct ctctgacatt catgaaaagt ttagagtgag
    16141 gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat
    16201 acacatttgt ctgataagct aggttcagct ttgcttgaac gattggctca cgtacttgtc
    16261 gttccttcac gatattcact tgaggttggg tataaaacag atctggataa actgtttccc
    16321 gcgactcaaa tggtaattgc ccaactgctt cagctgcttt gtccggatca acatcgccga
    16381 caacaaaaat gtcgatttga tcatttgcca tcatttcttg atagtaggac gctagcgaac
    16441 tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc tgggatcttt tgttccgggc
    16501 tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa agtttgttta tcttccttca
    16561 aactttccaa atatagacgc agattgttct tttccaagtc aaacgtcaac tgatcaaact
    16621 gatgattttt aatatttgga taaaataaaa cctcttttaa aaaatctacc gcttgagaaa
    16681 aaaggtttgg atcattgaca tattcaccat tcacaaaact gatccctgcg tttagccaat
    16741 gtaaatttcc tttttttcta actgataatc caaagcttgc gccataaagc tctgcaagtt
    16801 gcgcacttaa ctttgtctga tccgaataat tcaaactatt tgtttccaac atgctgctga
    16861 gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc
    16921 ggatcgtttt gtattgtttt gttggtaaga catgaagatt cacgccttct gccaattgat
    16981 acaccatatt tatatgctcc ttttcacagt tgattgcccg cttactcttt taatgggtca
    17041 acctgccttt tttacttatt cctctgcttg aaatttataa gttcaggcaa ttcgccgttt
    17101 tttctaaatg atgttacact ttatagtata gcatagatgt tttttatcaa aagaaacaga
    17161 cggctaaaag cgtatattgt ttttttattt tttatttttc gatatactaa agacaaataa
    17221 aagaagggag ggtcattcat gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc
    17281 ttggatcttg ctgtcggcgt tgttattggt agtgcgttta caagcatcgt aaaccaagtg
    17341 gtgcaaggct tgatcacacc actaataggt tggtttgtcg caatgatcac aggtacttct
    17401 gatttagaag gagcactttc aattcttgac tggtcgccga caaaaggcgt cacatttgcg
    17461 tttggtaacg ttattagtgc tatcattaca tttctgatca ctggttttgt cttattctta
    17521 gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca aagaagagca agtggaagtg
    17581 gaagaaactc ctactgcaga agattatctt agtgacatac gcgatcttct tgccaaacaa
    17641 gtggcagaag acaatacaca tccagtagag caaacaaatc tttccgatac ttctaaataa
    17701 taaagaatct aaaaaaaaag aaagccgatt ttcagtgtcg gctttctttt tttagattct
    17761 taacttgcta cttgttcctt tttcatgtcg atatgtggaa tatcatcttc caaatacaca
    17821 tctgagattg ccttgaatcc aaacgactca taaaaagctt gtaggtattc ttgtgcgccg
    17881 atcacgattg gtttttctgg aaaatttttc tggatcatgt taatcgtttc ttgaacaatc
    17941 tttcttccta aaccatcttt acggttgtcc tgatgaacaa ggacacgtcc aaaatggata
    18001 ctctccgctt cttcatatac tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa
    18061 aacgtatgcc aagcttctcc atcaatgtca tcaacttctt gataaggaca ctgttgttcc
    18121 acaacaaaaa ctgcgattcg ttctttgacg atcgcgtaaa actctgctgt tgacaattct
    18181 tcaaatttct ttacctgata ttccactaag caaacgcccc ctgctttttt attcttagca
    18241 taccgattcg cacacaaaaa agcaaacgaa ccttacgaat ctcaggctcg tttgcttttt
    18301 aaactgcttc agttaaaaat tcgatctcaa ctgtccgagt aagtacatct gaatagctgt
    18361 aagatactcg ttcaaaagaa ttttcttcag gatccaaatc aacaacgaat actgaaggat
    18421 aggtttctgt taaaacgccg cggcgttctg tttgacgctt tcttcctgtc tgagcaacca
    18481 acatgattgg gctgccgata cgaccttcta aatctttctt gattgaagct aaagttgttg
    18541 gcatacagtt cacctctact atcaactata ccacatattt t
//
LOCUS       DARBEG010000054        18158 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000054 DARBEG010000000
VERSION     DARBEG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.054"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..152
                     /locus_tag="SCK20_001826"
     CDS             <1..152
                     /locus_tag="SCK20_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145875.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="SCK20_001827"
     CDS             complement(230..763)
                     /locus_tag="SCK20_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG1145876.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCK20_001828"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCK20_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG1145877.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCK20_001829"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCK20_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG1145878.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="SCK20_001830"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="SCK20_001830"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1145879.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="SCK20_001831"
     CDS             complement(4488..5066)
                     /locus_tag="SCK20_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG1145880.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="SCK20_001832"
     CDS             5388..5858
                     /locus_tag="SCK20_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG1145881.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="SCK20_001833"
     CDS             complement(5966..7192)
                     /locus_tag="SCK20_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1145882.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="SCK20_001834"
     CDS             complement(7946..8146)
                     /locus_tag="SCK20_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1145883.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="SCK20_001835"
     CDS             complement(8280..8888)
                     /locus_tag="SCK20_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG1145884.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="SCK20_001836"
     CDS             complement(8973..9443)
                     /locus_tag="SCK20_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG1145885.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="SCK20_001837"
     CDS             complement(9491..10405)
                     /locus_tag="SCK20_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1145886.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="SCK20_001838"
     CDS             10551..11354
                     /locus_tag="SCK20_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG1145887.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="SCK20_001839"
     CDS             11532..12479
                     /locus_tag="SCK20_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG1145888.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="SCK20_001840"
     CDS             complement(12547..12840)
                     /locus_tag="SCK20_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG1145889.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="SCK20_001841"
     CDS             complement(12868..13533)
                     /locus_tag="SCK20_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG1145890.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="SCK20_001842"
     CDS             complement(13673..14305)
                     /locus_tag="SCK20_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1145891.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="SCK20_001843"
     CDS             complement(14312..15379)
                     /locus_tag="SCK20_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1145892.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="SCK20_001844"
     CDS             complement(15376..16107)
                     /locus_tag="SCK20_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG1145893.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCK20_001845"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCK20_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG1145894.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCK20_001846"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCK20_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG1145895.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5386 a         3684 c         2904 g         6184 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaa
//
LOCUS       DARBEG010000055        17661 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000055 DARBEG010000000
VERSION     DARBEG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.055"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1475
                     /locus_tag="SCK20_001847"
     CDS             138..1475
                     /locus_tag="SCK20_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG1145896.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="SCK20_001848"
     CDS             1523..3652
                     /locus_tag="SCK20_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG1145897.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="SCK20_001849"
     CDS             3627..4316
                     /locus_tag="SCK20_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145898.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="SCK20_001850"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="SCK20_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="SCK20_001851"
     CDS             complement(5546..5740)
                     /locus_tag="SCK20_001851"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145899.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="SCK20_001852"
     CDS             complement(5790..6230)
                     /locus_tag="SCK20_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG1145900.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="SCK20_001853"
     CDS             complement(6234..7079)
                     /locus_tag="SCK20_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG1145901.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="SCK20_001854"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="SCK20_001854"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG1145902.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="SCK20_001855"
     CDS             complement(8703..9650)
                     /locus_tag="SCK20_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1145903.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="SCK20_001856"
     CDS             complement(9873..10223)
                     /locus_tag="SCK20_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1145904.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="SCK20_001857"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="SCK20_001857"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG1145905.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="SCK20_001858"
     CDS             11646..11966
                     /locus_tag="SCK20_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1145906.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="SCK20_001859"
     CDS             11988..12452
                     /locus_tag="SCK20_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1145907.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="SCK20_001860"
     CDS             12657..13262
                     /locus_tag="SCK20_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG1145908.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="SCK20_001861"
     CDS             complement(13301..14590)
                     /locus_tag="SCK20_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG1145909.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="SCK20_001862"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="SCK20_001862"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG1145910.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16250
                     /locus_tag="SCK20_001863"
                     /pseudo
     CDS             15700..16250
                     /locus_tag="SCK20_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16321..17490
                     /locus_tag="SCK20_001864"
     CDS             16321..17490
                     /locus_tag="SCK20_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG1145911.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         5660 a         3179 c         3541 g         5281 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta ccaagcaagt
    17161 tcaaaagaag aagcaaataa tcaaataagg tttatgatag ataaatggaa aaagcagtat
    17221 ccacgagtgg ttaaattgct catgaatcct gctatattaa ccttttataa tttccctccg
    17281 tcaatcagaa gaactattta ttcaactaat ttgattgaag gttttaacaa acaattaaag
    17341 aaatatacca aaagaaaaga gcaatttcct aatgaagaat ctctggagag atttctagta
    17401 tctcaattca acaactataa tcaaaagttt ctatgccgag ttcataaagg ttttaaagaa
    17461 atacatgata cattagaatc aatgttttaa cttgatcaac agaatgggct tttccattta
    17521 cacaaaatta ttgacgctcc cgacaaaatt atgatcttgc gtaaagttat aaaatattaa
    17581 taaaaatatt ttattaatga aaagcttggt agcacggtta ttcatatgct aatttatttc
    17641 actttatagt aaggggagtt c
//
LOCUS       DARBEG010000056        17226 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000056 DARBEG010000000
VERSION     DARBEG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.056"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            2..370
                     /locus_tag="SCK20_001865"
     CDS             2..370
                     /locus_tag="SCK20_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG1145912.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="SCK20_001866"
     CDS             550..1491
                     /locus_tag="SCK20_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG1145913.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="SCK20_001867"
     CDS             1560..2291
                     /locus_tag="SCK20_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG1145914.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="SCK20_001868"
     CDS             2435..4615
                     /locus_tag="SCK20_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG1145915.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="SCK20_001869"
     CDS             4635..5117
                     /locus_tag="SCK20_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG1145916.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="SCK20_001870"
     CDS             5133..5894
                     /locus_tag="SCK20_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1145917.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /locus_tag="SCK20_001871"
     CDS             6025..7359
                     /locus_tag="SCK20_001871"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG1145918.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="SCK20_001872"
     CDS             7666..8151
                     /locus_tag="SCK20_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG1145919.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="SCK20_001873"
     CDS             8144..8827
                     /locus_tag="SCK20_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG1145920.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="SCK20_001874"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="SCK20_001874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG1145921.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="SCK20_001875"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="SCK20_001875"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG1145922.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /locus_tag="SCK20_001876"
     CDS             12443..13072
                     /locus_tag="SCK20_001876"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG1145923.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="SCK20_001877"
     CDS             13124..13456
                     /locus_tag="SCK20_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1145924.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="SCK20_001878"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="SCK20_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG1145925.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="SCK20_001879"
     CDS             14282..15655
                     /locus_tag="SCK20_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1145926.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="SCK20_001880"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="SCK20_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG1145927.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="SCK20_001881"
     CDS             16710..17150
                     /locus_tag="SCK20_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1145928.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5875 a         3009 c         3796 g         4546 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atggga
//
LOCUS       DARBEG010000057        16948 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000057 DARBEG010000000
VERSION     DARBEG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.057"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..3164)
                     /locus_tag="SCK20_001882"
     CDS             complement(459..3164)
                     /locus_tag="SCK20_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG1145929.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(3470..4417)
                     /locus_tag="SCK20_001883"
     CDS             complement(3470..4417)
                     /locus_tag="SCK20_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG1145930.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            4607..4876
                     /gene="rpsN"
                     /locus_tag="SCK20_001884"
     CDS             4607..4876
                     /gene="rpsN"
                     /locus_tag="SCK20_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1145931.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(5052..5414)
                     /locus_tag="SCK20_001885"
     CDS             complement(5052..5414)
                     /locus_tag="SCK20_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1145932.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(5425..6546)
                     /locus_tag="SCK20_001886"
     CDS             complement(5425..6546)
                     /locus_tag="SCK20_001886"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG1145933.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(6562..6912)
                     /locus_tag="SCK20_001887"
     CDS             complement(6562..6912)
                     /locus_tag="SCK20_001887"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG1145934.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(7042..8553)
                     /locus_tag="SCK20_001888"
     CDS             complement(7042..8553)
                     /locus_tag="SCK20_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1145935.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(8875..10227)
                     /locus_tag="SCK20_001889"
     CDS             complement(8875..10227)
                     /locus_tag="SCK20_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG1145936.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(10380..11456)
                     /locus_tag="SCK20_001890"
     CDS             complement(10380..11456)
                     /locus_tag="SCK20_001890"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG1145937.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            11652..13397
                     /locus_tag="SCK20_001891"
     CDS             11652..13397
                     /locus_tag="SCK20_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG1145938.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(13677..14114)
                     /locus_tag="SCK20_001892"
     CDS             complement(13677..14114)
                     /locus_tag="SCK20_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG1145939.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(14098..15471)
                     /locus_tag="SCK20_001893"
     CDS             complement(14098..15471)
                     /locus_tag="SCK20_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145940.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(15793..16281)
                     /locus_tag="SCK20_001894"
     CDS             complement(15793..16281)
                     /locus_tag="SCK20_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1145941.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(16303..16905)
                     /locus_tag="SCK20_001895"
     CDS             complement(16303..16905)
                     /locus_tag="SCK20_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1145942.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         4766 a         3624 c         3076 g         5482 t
ORIGIN      
        1 caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta tgggcttttt
       61 ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc tatccttttt
      121 gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat tcttactact
      181 cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc ttgcatctgc
      241 ggtaataagc ccaaccaaat taaaaacata agaagctgtt tttatttggt cgttcttatt
      301 acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat cagctgaccc
      361 tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt tttttctcta
      421 atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat atggctctca
      481 tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat tgccgttcct
      541 acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt agcatttggc
      601 caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa tggcaagaac
      661 ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc gcctccgcca
      721 gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc gaataatgca
      781 gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa tgacacgctg
      841 tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt aacgatcaag
      901 ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg ttctcgtttc
      961 gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac acttgaaaga
     1021 agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc tgaaccgtta
     1081 tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa gaagtcactt
     1141 tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac ttcttgttcg
     1201 ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg ccagtcattg
     1261 ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt ttcatttact
     1321 gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg gataaaatca
     1381 gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag gtctgcccct
     1441 tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt ggcatcatgg
     1501 atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc cactgcattc
     1561 gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc cgctttgctc
     1621 agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga agtgacagtg
     1681 gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt aacaacggca
     1741 tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac agctgtgttc
     1801 aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt tagctgatca
     1861 ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag gaactcgacc
     1921 agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt caatacattg
     1981 ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt tcttacagta
     2041 tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt aacatctaca
     2101 ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt tgtttggttt
     2161 gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc ttgtacttgt
     2221 tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt ctcttggatc
     2281 gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat ctctgggatt
     2341 ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat tttgtcattc
     2401 aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac gaattcattt
     2461 gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag gacttgtttt
     2521 gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc tttaacttta
     2581 ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac tttcaataaa
     2641 gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga acttgctcct
     2701 ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata ttcgattttt
     2761 ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc ctttggcaag
     2821 taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc ttttgaatca
     2881 acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg ctccccaatg
     2941 gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc aattggcagc
     3001 tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc atatagcgaa
     3061 gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa aatccgtttc
     3121 cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct cccccttctt
     3181 cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg gcttttttca
     3241 aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc ttacatattg
     3301 agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa agataatttt
     3361 atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt ttttgtcttt
     3421 gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct tatttttcga
     3481 atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt gcttggatga
     3541 catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct cctttttggt
     3601 aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt ggaacaaacg
     3661 attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca tttaatgcaa
     3721 catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata cttcctttcg
     3781 tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt gttttatcat
     3841 aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta ctggatgcac
     3901 tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac ccgaccgcac
     3961 ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa tggttgatta
     4021 gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa ctcattttat
     4081 ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa gtcccgccga
     4141 ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa ccaatctctg
     4201 tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt cgtatctctt
     4261 tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct tttatttcta
     4321 cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca aaaacatctt
     4381 gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc ctttttcttt
     4441 aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt ggcgcatgat
     4501 tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc taaaagcgta
     4561 aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg ctaaaaaatc
     4621 aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg ctttacgcca
     4681 ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag atgctcgtcc
     4741 agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca tgcgtaaatt
     4801 cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc caggagtaaa
     4861 aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg catggaccac
     4921 tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg gtactagaag
     4981 cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc tgcatggtta
     5041 cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact aattttcaat
     5101 gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc tttcaatctt
     5161 gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc tattcctgtt
     5221 ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt tgccgttatg
     5281 gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat aaccaaaact
     5341 gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa ataaatatct
     5401 cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc gctgtcctaa
     5461 tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct gcatagtgat
     5521 ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa attcttgagg
     5581 caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt agttcccttc
     5641 taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac cgatcggtat
     5701 cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga ttccttcacc
     5761 tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg
     5821 ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta ctccataacg
     5881 aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc tgacatgtac
     5941 atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt tttgcagatt
     6001 ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac cttcccattc
     6061 taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa
     6121 tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg tctctagctt
     6181 acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg cagttactga
     6241 taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca agataaggat
     6301 gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg aaacacaaaa
     6361 gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat gaccgtatcc
     6421 gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa cttcatttgc
     6481 tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt gccaagcgac
     6541 aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt
     6601 tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca caacaggctg
     6661 accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga tcccagttcc
     6721 ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg
     6781 atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc gtgcaatgaa
     6841 ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat cgatcccaat
     6901 tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat tctaccaaat
     6961 tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt ctcttaaaaa
     7021 agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc cgcgtttttt
     7081 atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat aaccgccttt
     7141 tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc cgccaccacg
     7201 tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg gtgtgatctt
     7261 cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg caactaaatc
     7321 ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat ctagaccatt
     7381 ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt tgaatgcttc
     7441 tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt caatcacatg
     7501 caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt ttccggcacg
     7561 gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat catagttgta
     7621 cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg ctacaaggat
     7681 atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc
     7741 gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac
     7801 acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat caaacaagcg
     7861 agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat cgatcaagtc
     7921 agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga atttaacgcc
     7981 aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg
     8041 aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca tttcatctgc
     8101 ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt taatgtggtc
     8161 aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc cacggatctg
     8221 acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa
     8281 acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga taactaaacc
     8341 ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa atgcagctgt
     8401 tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg ctagtggaat
     8461 cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa
     8521 ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg gttgttaact
     8581 gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat tgtttctttt
     8641 tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac cgaaaaatca
     8701 ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt atcattatta
     8761 tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc tcttcatctt
     8821 tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag atttttattt
     8881 catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc cttttagcat
     8941 aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat tcttttcttt
     9001 ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt ttagtgcatg
     9061 catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat gttcactcat
     9121 ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta acacagcgat
     9181 tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca tggctgttgg
     9241 atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta gccattcggt
     9301 tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta tggacagtcc
     9361 aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac ttccaggcag
     9421 cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc cttttgctgt
     9481 cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt gtactaatgt
     9541 tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc cgtctgatac
     9601 aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc caagattttc
     9661 tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca taagtgaaag
     9721 ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt ctgtatcttg
     9781 gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc cttgcgtctt
     9841 atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg tggttttacc
     9901 attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat aggttgccaa
     9961 ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt ttggagcttg
    10021 ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt gctggataaa
    10081 ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg ttatcttccg
    10141 gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc ccacggcttg
    10201 tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt gtgaaaaaag
    10261 cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt ctctattcgt
    10321 atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt tatttctctt
    10381 tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc taactggatc
    10441 agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag tgggtacata
    10501 ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc atttttattt
    10561 gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta agctaacttc
    10621 gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat ctgcatttcg
    10681 attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca
    10741 ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct
    10801 tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt
    10861 tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt
    10921 ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt
    10981 tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt
    11041 aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg
    11101 acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc
    11161 ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact
    11221 tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct
    11281 ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata
    11341 aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa
    11401 agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga
    11461 ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa
    11521 aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt
    11581 tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag
    11641 gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt
    11701 tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa
    11761 aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag
    11821 aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta
    11881 gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag
    11941 atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc
    12001 gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg
    12061 aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc
    12121 ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca
    12181 atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg
    12241 gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg
    12301 tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg
    12361 aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag
    12421 ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag
    12481 ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg
    12541 aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag
    12601 accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg
    12661 caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc
    12721 gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt
    12781 tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca
    12841 acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg
    12901 atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc
    12961 tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca
    13021 ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga
    13081 aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac
    13141 aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta
    13201 tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc
    13261 ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt
    13321 atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata
    13381 agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag
    13441 gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg
    13501 ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag
    13561 aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt
    13621 attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag
    13681 tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt
    13741 ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga
    13801 agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc
    13861 ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta
    13921 accataccga cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg
    13981 acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca
    14041 agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca
    14101 atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct
    14161 cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg
    14221 cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc
    14281 taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt
    14341 agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc
    14401 cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc
    14461 aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc
    14521 caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa
    14581 gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc
    14641 aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa
    14701 cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa
    14761 caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa
    14821 caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc
    14881 tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg
    14941 actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat
    15001 ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa
    15061 taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt
    15121 ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa
    15181 taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag
    15241 agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag
    15301 tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga
    15361 gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa
    15421 gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc
    15481 tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac
    15541 caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa gcttctttct
    15601 gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc
    15661 gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat
    15721 caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca
    15781 cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt gaaatccttg
    15841 accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat aaacctcgat
    15901 tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg tttgcgcagc
    15961 aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta ctttatttag
    16021 caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag tgtaaccgac
    16081 agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt ctttttgaat
    16141 ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac caatgatcgg
    16201 cacaaaaact tccggccata cttcttgtat gactgccatc agctcatcca gttcattttc
    16261 tgttactttt attttttcca tacacccacg tcctttttta cattataaat gaccttgcgc
    16321 ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg gtttcaagaa
    16381 aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta ctagacagat
    16441 caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt taatatcttt
    16501 cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata aaataagctg
    16561 gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc caatcactag
    16621 gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc ccgctactga
    16681 ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca gaacaaatat
    16741 catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa
    16801 aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac caataatggg
    16861 catgatctga agacattttt gtaaaactga tcctttttta gacatctata ttccccacat
    16921 cttttttcat ttattaaaac tgcctcct
//
LOCUS       DARBEG010000058        16530 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000058 DARBEG010000000
VERSION     DARBEG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.058"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(29..727)
                     /locus_tag="SCK20_001896"
     CDS             complement(29..727)
                     /locus_tag="SCK20_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG1145943.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="SCK20_001897"
     CDS             complement(720..2072)
                     /locus_tag="SCK20_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG1145944.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="SCK20_001898"
     CDS             complement(2295..3695)
                     /locus_tag="SCK20_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG1145945.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="SCK20_001899"
     CDS             3856..4593
                     /locus_tag="SCK20_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG1145946.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="SCK20_001900"
     CDS             4718..5197
                     /locus_tag="SCK20_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG1145947.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="SCK20_001901"
     CDS             5194..5961
                     /locus_tag="SCK20_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145948.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="SCK20_001902"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="SCK20_001902"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1145949.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="SCK20_001903"
     CDS             complement(6864..7466)
                     /locus_tag="SCK20_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1145950.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..8041
                     /locus_tag="SCK20_001904"
                     /pseudo
     CDS             7572..8041
                     /locus_tag="SCK20_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8147..8731
                     /locus_tag="SCK20_001905"
     CDS             8147..8731
                     /locus_tag="SCK20_001905"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1145951.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="SCK20_001906"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="SCK20_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG1145952.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..10639
                     /gene="prmC"
                     /locus_tag="SCK20_001907"
     CDS             9800..10639
                     /gene="prmC"
                     /locus_tag="SCK20_001907"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG1145953.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10632..10811
                     /locus_tag="SCK20_001908"
     CDS             10632..10811
                     /locus_tag="SCK20_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145954.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10833..11858
                     /locus_tag="SCK20_001909"
     CDS             10833..11858
                     /locus_tag="SCK20_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG1145955.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12033..13277
                     /locus_tag="SCK20_001910"
     CDS             12033..13277
                     /locus_tag="SCK20_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG1145956.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13430..14377
                     /locus_tag="SCK20_001911"
     CDS             13430..14377
                     /locus_tag="SCK20_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1145957.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14511..15140
                     /gene="upp"
                     /locus_tag="SCK20_001912"
     CDS             14511..15140
                     /gene="upp"
                     /locus_tag="SCK20_001912"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG1145958.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15279..15521)
                     /locus_tag="SCK20_001913"
     CDS             complement(15279..15521)
                     /locus_tag="SCK20_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145959.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15642..15714
                     /locus_tag="SCK20_001914"
     tRNA            15642..15714
                     /locus_tag="SCK20_001914"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Thr,seq:cgt)
     gene            complement(15833..16471)
                     /locus_tag="SCK20_001915"
     CDS             complement(15833..16471)
                     /locus_tag="SCK20_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1145960.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         5637 a         2949 c         3324 g         4620 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaaaggat ctttttggca atgaacgaat
    10621 gatccaagtt tatggttgaa gttgtggatg aaaaattttt tatttgtttt tttattataa
    10681 aaaaacactt atttgacaag gatgtaaacg actttttggt ttatcaacaa gtttatccac
    10741 tattgacaaa tagattatcc acaaacagtg gaaaacttga ggataaactc agacaaaata
    10801 agaatgtatg aaaaaggggt tgtggataaa ctgtggaaac gaaacgatat gatgaaacag
    10861 aattgcaaga ggcagccgaa gcactgaaag aaggagaact tgttgctttt cctaccgaaa
    10921 cagtatacgg attgggcgcc aacgctcttt taccaaacgc tgtcaagaaa gtgttttctg
    10981 taaaagggag accgcaagat aatccattaa tcgtccatgt cgcctctttt gaacaagtaa
    11041 aagagtatgt ggacaacttt catccggcag cagagaagat tgtgaagaac ttctggcctg
    11101 gaccattaac tttgatattt aagatcaaaa aagacacttt gccctcagtt gtgacgggag
    11161 gattatccac agctgcattt cgtataccgg ataacaaaaa aacactagaa gtaatcgaac
    11221 tttccggtgt tccgcttgtc ggtccaagtg caaatacgtc gggaaaacca agtccaacga
    11281 ctgcagatca tgtatatcat gatctacaag gaaaaattac cgggattatt gatgatggag
    11341 ctacgcgaat tggtgtagaa tccactgttc ttgatttaag tgatccaaca gccatgccta
    11401 tgattttacg accaggtgca gtaacgaaag aacaaatcga agcagtcatc gaatctcctg
    11461 ttgctattga tcagcatctt gtaaaagaaa atgaaacacc aaaagcccct gggatgaaat
    11521 acaaacacta ctcacccgat acacgtgttc tgatggtcag agatggcgat tggtcaactg
    11581 cagttcaatg ggcaaaaaat aagaagatcc gtgcaggtgt catagcaagc cccgaaattg
    11641 cggatcaagt gcggacagat actgcagctg tttatatgta taacgacaac tcagtagaag
    11701 cggcggctaa aggattgttt gcaggtctaa gaggattaga tgaaccaaca cttggattag
    11761 atttgatttt tgtacaagtg tatccagaaa ctggattggg gaatgcttac atgaatcgct
    11821 tgaaaaaagc agcaggacaa aattattttg aaaaatgatc aattggttat tgtcgcattc
    11881 ttttagtatg atacaatgct atcaagtaaa tcttctatca gtgaacgaaa aactaagcgt
    11941 aactaatcgt aatagaagag atacgaatat ttggcaattt gtttttgatg ggaaaaataa
    12001 aattgtagga aaataaaaag gagctgttgt ttgtggtaga ttacaaaacg tttgaccctg
    12061 atctttgggc tgcgatcgcc aaagaagaag aaagacaaga acataatttg gagctgattg
    12121 cctctgaaaa ctttgtctcc gaagcagtga tggctgcaca aggaagtatc ttgacaaata
    12181 aatacgcaga aggttatcca gggcatcgct attatggtgg gtgtgaattt gtcgatatcg
    12241 ttgaaaattt agcgattgac cgtgcaaaag aattattcgg agcgaaattt gcgaacgtcc
    12301 agcctcactc cggctctcaa gccaacacgg ctgcttatct cgctttggta gaaccagggg
    12361 acacgatctt aggaatggat ctttccgcag gcggacatct aacacatgga tcaccagtca
    12421 acttcagtgg caaaacctat cactttgttg cttacggtgt tgatccgaca acagaagtta
    12481 ttgattataa tgtggtacgt attttagcta gaaaacatca accaaaattg atcgtagcag
    12541 gtgccagtgc ttacggacga acgatcgact ttgcgaaatt cagagaaatc gcagatgaag
    12601 taggcgcaaa actgatggta gatatggcac atatcgcagg attagtagca gcaggacttc
    12661 atcctaatcc agttccttat gcagatatca caacgactac cactcataaa acattgcgtg
    12721 gccctcgagg cggcatgatt ttgacaaacg atgaagcatt agcaaaaaaa ataaatagtg
    12781 cggtatttcc tggtattcaa ggcggccctc tagagcatgt gattgcgggt aaagcagtag
    12841 cattcaaaga agcattagat cctgcattta aagaatatag tgaacaaatc atcgccaatg
    12901 caaaagcaat ggtcaaagtt ttcaatcaag caattggaac cagagtcatt tcaggggcga
    12961 cagataatca tctgatgttg atcgatgttc gcgagttagg tataaatgga aaagaagcag
    13021 agagcatttt ggacagtgtg aatatcactg taaataaaaa ctcgattcct tttgaaacac
    13081 taagtccatt caaaacaagc ggtattcgaa tcggcacgcc agcaatcact actcgcgggt
    13141 tcaaagaaga agatgctgta aaagtggctg aactagttgt caaagcatta caagcaaaag
    13201 atgataacgc tcaactagac gaagtcaaaa caggtgtacg cgaattaact gaaaaatttc
    13261 cgttgcataa aaaataaaaa tttaaattcc attagtaaac agaaaagttc aatgttgggc
    13321 ttttctgttt attttttggg aaaacggatt ctttgacaat atcggtgatg ctctctagta
    13381 tatgaggtgt aattttagtc ttcacactaa atctcaggga ggaaaagtca tgactgatcg
    13441 tatctcgtta gaaaaggaag cagtagcatt cagtgatgca aatgcgcctc atccgcgtat
    13501 ctatgaacta gcaccggaag aaggaagagt tttactagaa gaagtacaag aatcacctgt
    13561 gaacaaatat tccgtcgata ttgaagactt gatggtggat actggcgaat ggggaaaact
    13621 caatattcgt ctcgtacgac cagaagggaa tatggataaa ttaccagtta ttttttatat
    13681 tcatggagca ggctgggttt ttggcagtgc tcaaacccat gataaattga tcagagaact
    13741 tgcggttcgt acgaattccg tcgttgtctt tccagaatat agtcgatcac cagaagcaaa
    13801 atatccaaca gcaattgaac aaaattatgc tgtgttgcaa cagctgaata aattagccaa
    13861 gtcaaaaaat ctggatctcg agaatctaat cgttgcagga gattcagtag gtggtaacat
    13921 ggctacagtc atgacgatct taacaaaaga acgaaagggc ctcccaatat ccaagcaatt
    13981 gctgtattat ccagtcacaa acgctgattt tgatacagat tcctatcatg aattcggaga
    14041 aaactatttt ttgacaagag aaggaatgat gtggttttgg gaccaatata cgacgaatga
    14101 agaagagcgt gctgaaatca cagcttcgcc tttgagagca aatataaatg aactaaatga
    14161 tcttccttca gcaatgattt taacgggaga agcggatgta ttaagagatg aaggtgaggc
    14221 ttatgcgcgc aaacttcgtg atgcaggggt tcctgttaca caagtacgct tccaaggaat
    14281 catccatgat tttgtcatgg taaatgctat ggatcagaca aaggcaacgc gtgcagcaat
    14341 ggcattatct acttcttgga tcaagaaaaa agaataagct ttgcttgtcc gcaggaaaac
    14401 ttcttttcaa aaacggattt tcttaggaaa aatggcgagt ttgactagtc aatcacttga
    14461 ttttgtttta caattaaaaa gaaacgacag aacaaaggag agcttaaaaa atgggcaaat
    14521 ttcaagtgat cgatcatcca ttgattcaac acaaactaac tattattcgg gacaaaaact
    14581 gcggaacaaa ggtttttcgt gaagtagtcg atgaaatcgc tatgttgatg gcatatgaag
    14641 tatcacggga tatgccattg gaagacatcg tgatcgaaac accgatcact gaaacaacac
    14701 aaaaaacgtt gtctggtaaa aaagtagcga ttattccaat cttgcgcgca ggtattggaa
    14761 tggttgacgg tatcttggaa ttgattccag ctgctaaagt gggacatatc ggtctttatc
    14821 gagatgaaga aacattggaa ccacatgaat atttcgtcaa acttccagaa gacattgatg
    14881 cacgtcagtt gtttgtagta gatccaatgt tggcaacagg cggatcagca atcatggcaa
    14941 tcgatgcatt gaaagaacgt ggcgcgtcga acattaaatt tgtttgtttg gtagcagcac
    15001 ctgagggagt aaaagctttg caagaagcgc atcctgatat tgacatctat acagcttctc
    15061 tagatgaaaa attgaatgaa catggttata tcgttcctgg actaggggat gcgggtgacc
    15121 gtttgtttgg tacaaaataa gtaaatacga aaaagggtgt gacacaactt ttgtcacatc
    15181 cttttttggt catttttaac gtttgatgca gaacagcttt ttcacagctt tcgtttgcat
    15241 ataaaaaaac catcccaaaa atgcagagat ggtcaaattc aaatcttttt tagttctttg
    15301 tagttaacga ttactacacc cttactttgc gtcatcaagt tcgcattttc tgcagtttca
    15361 tcaatctcaa caacagcgga atttttcaat tgctttgaaa tcgttccagt ttgcgacttg
    15421 ccgtgaattg tgaacattac gtgagtattg atcttgaatt tttcaccaat ttccgatggt
    15481 acgatagttg tatcaaattt tccaaaagtt gccattttca taaaacctgc ctttcacatc
    15541 aattatacca cgttttatga aaaatatcat gaaattaaac taattttcca tcggactctt
    15601 tacaatcagc caaatagagt atataatttc ttttggataa ggccagctta gctcagttgg
    15661 tagagcaacg cactcgtaac gcgtaggtcg taggttcgat ccctatagct ggcattaaaa
    15721 gagaagacag atatatcaag gatttttaat ccattgatac atctgtcttt ttgtttaaag
    15781 gaaataaagt atgctttctt agcaatagag catgtattat ttctataaaa atttaggaga
    15841 ataaaacgac tagcaatata gctgtgaaat caaatatcac atgaactgcc caacttgtaa
    15901 tgatcgagtt gcttttaatg gcagcttggt tgaataacaa tcttgccacc ccttgtatca
    15961 aaaggatgtg tgctacagtg tgtaaaatat ttccattatc ataagtactg taatggataa
    16021 atccaaagat gattgctgac aagaaactag cgacaggaat gctgaactta ctagaaaaaa
    16081 tagcgagaaa atacactgag aacaattctt ctccaagtat catgataggc aaaataataa
    16141 ataataacgg gctgaaagcc tcatttattg gattgcctgt taaaccccat ttcaaaattt
    16201 gttgcaaaaa taccgagact gcggcactga ttactacatt gataaaaaaa tatttggcta
    16261 tatttttgac aggagcgaca ggtttcgaga acattttttt cgtatctttt ttaccaaaaa
    16321 caagtgtaaa tgctataaat ccagtgatag ttaaagaaaa aatgctaata atggatggcg
    16381 caaatagcaa ccctaacaaa ccgagtacaa cgacaatagt gcccaatatc ccttttttaa
    16441 gttctatttt ttttcctaaa atgttattca tgataataac tcctactatt cagtttttga
    16501 agatagagtc agtatacatg aagagttaaa
//
LOCUS       DARBEG010000059        16273 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000059 DARBEG010000000
VERSION     DARBEG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.059"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..554)
                     /locus_tag="SCK20_001916"
     CDS             complement(57..554)
                     /locus_tag="SCK20_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG1145961.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(684..1394)
                     /gene="budA"
                     /locus_tag="SCK20_001917"
     CDS             complement(684..1394)
                     /gene="budA"
                     /locus_tag="SCK20_001917"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG1145962.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1407..3071)
                     /gene="alsS"
                     /locus_tag="SCK20_001918"
     CDS             complement(1407..3071)
                     /gene="alsS"
                     /locus_tag="SCK20_001918"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG1145963.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3279..3941)
                     /locus_tag="SCK20_001919"
     CDS             complement(3279..3941)
                     /locus_tag="SCK20_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1145964.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3951..4766)
                     /locus_tag="SCK20_001920"
     CDS             complement(3951..4766)
                     /locus_tag="SCK20_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1145965.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5027..5470)
                     /locus_tag="SCK20_001921"
     CDS             complement(5027..5470)
                     /locus_tag="SCK20_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG1145966.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5604..5942)
                     /locus_tag="SCK20_001922"
                     /pseudo
     CDS             complement(5604..5942)
                     /locus_tag="SCK20_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5930..6307)
                     /locus_tag="SCK20_001923"
     CDS             complement(5930..6307)
                     /locus_tag="SCK20_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1145967.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6531..7724
                     /locus_tag="SCK20_001924"
     CDS             6531..7724
                     /locus_tag="SCK20_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1145968.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7890..8312)
                     /locus_tag="SCK20_001925"
     CDS             complement(7890..8312)
                     /locus_tag="SCK20_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1145969.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8895..9389
                     /locus_tag="SCK20_001926"
     CDS             8895..9389
                     /locus_tag="SCK20_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145970.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(9551..10723)
                     /locus_tag="SCK20_001927"
     CDS             complement(9551..10723)
                     /locus_tag="SCK20_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1145971.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10929..11744)
                     /locus_tag="SCK20_001928"
     CDS             complement(10929..11744)
                     /locus_tag="SCK20_001928"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1145972.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(11894..12340)
                     /locus_tag="SCK20_001929"
     CDS             complement(11894..12340)
                     /locus_tag="SCK20_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1145973.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(12337..13341)
                     /locus_tag="SCK20_001930"
     CDS             complement(12337..13341)
                     /locus_tag="SCK20_001930"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG1145974.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(13381..13710)
                     /locus_tag="SCK20_001931"
     CDS             complement(13381..13710)
                     /locus_tag="SCK20_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1145975.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(13836..16163)
                     /locus_tag="SCK20_001932"
     CDS             complement(13836..16163)
                     /locus_tag="SCK20_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1145976.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4638 a         3245 c         2887 g         5503 t
ORIGIN      
        1 tctttccggc tctttttatt cttgatttct cttctttttt gtttcaatag acgattttag
       61 ttattaataa atgaaaaagc atttatatta aaatttgcta ataaaagatc tacatatttt
      121 tctagaaatg cttgatcgat ttcatcatac cctttcgtta ctccgcaatc gatatctaaa
      181 acaccgatta aattaccttc ttttaccatt ggcactacga tttctgattg tgcggctgaa
      241 tcgcaggaaa tgtaatttgc atgggttttg acatcatcaa cgatcaatgt ttcttgtttt
      301 tcagctgatt ctccacaaac tccttttccc atcttgatac gtgtgcaaga aactcgccct
      361 tggaacggtc ctaaaatcaa ttcattacca tcgaataaat aatatcctgc aaagacagtc
      421 tcaggcaaag tctcattcaa caatgcagat gaatttgcta aatttgctaa ccagttatct
      481 tctgcttcta ataatccttg ttgctgtaat aataaaattt cgtatgcatc ttttttctgt
      541 ttttctgtcc acatgataaa aaccgctcct ttttgatcta tggagaagat tatagctcag
      601 tttttttgta aacgctaggt ttcctcctca aaaaaacgag atacgtcagg ttaatcacct
      661 tgaacgcatc tcgctgtctt tttctattct gcttgttgga tttctgctgc taaatcagca
      721 taatcaatcg tttcgtgaac aaaagtcgga tcatcaactg gaaaatgctg tacaagggac
      781 gagatgcggc taatctcaac ttttccttca gccacttcaa aatccattac atgtccgcca
      841 aatgtatgtg tatcatcgat aaaatgcagg tgaaatcccg ctgcagcaac tccttggaat
      901 aattcaggcg taaagaaccc gactaatgtt ccttggatat tgctttgttg gaattctggt
      961 tgagactctg aaatttttgc tagtcttctg taaggttttt cttgcttcgg catgatacgg
     1021 atgtgcatat ttttgaataa tccagttatc ttcactgcca aaaataggtt ttccccggct
     1081 tctcttacta atatgtcttt tttcaattgt tctccatctg cagtttttga gacagaaaaa
     1141 ctagtatctg gcgtaaaatc tgtaattgct gcataggggg taagttcact tccatctaat
     1201 tgggcaactg agccatctga tcttccttga taagctacgc catctaaaaa gatgacttca
     1261 ccatcaagcc catgcaatgt tcccaatccc agagttcctt tttctaatat atgagcaatc
     1321 gtttctgttc catccatcaa gccggccatc aaagctccta atgtcccgtg ctggtataag
     1381 attttttcag tcatgtcgac acttccttaa tagaattgat ctggtaagat tgtttctccc
     1441 aatttctcat tatcacggta atcaatcgga atatcaataa ttacgggtcc ttctgtcgca
     1501 aatccttctt ctaaagcttt ttctaattcc tctacacttg tagctcggat tcctttagcg
     1561 ccaaaagctt ctgcatattt cacaaaatca actggaccaa agtctacacc tgacgcccgc
     1621 tgatatttca ttttttcttg gaactctacc atattgtagt gtccatcatt ccaaatcaaa
     1681 tgaacgatat tcaatttttt acgtaccgcc gtttctaaat cctgggcaga gaataaaaat
     1741 cccccatcgc cggaaactga tacgatttgt gtatttggac gcaccaatgc agcagaaatc
     1801 gcccatggca atgcaacacc aagcgtttgc ataccattac tgaataacaa atgacgaggt
     1861 tcataacttc taaagtggcg tgccatccaa atataatgac tacctacatc tacggtaaca
     1921 gtcatatcat ctgttacttt tgactgaagc gtgttaataa cttctaaagg atgcaagata
     1981 cctgcttctc cttttgattg aacaatgtcc cgctccgtta attttgcttg aagagatgct
     2041 aaatattctt ttgcatcttc tgacacttgt tgaggctcta atgaacctgt caataaatca
     2101 agagtggctg agatatcacc gatcaattct ctttcaggct gcataaaagg atcgatttca
     2161 gctggcgctt catcgatcac aatgatccgc gcatcttttt ccgcattcca gtttcttgct
     2221 tcatattcga ttggatcata gccaattgca ataactagat cacttcgttt caaaagcata
     2281 tctcctggtt ggtttctaaa taaacccaca cgaccaaaga aatgcgcttc taattcacgt
     2341 gagatcactc cagctgcttg gaatgtctct acaacaggca atgctgtctt ttcaactaat
     2401 tgcctgatgg ctaacgtttc tttttcactt gatcctcgca tacctactaa tagtaccggt
     2461 agttttgctt ctctaatgcg ttcagctagg taacggatat catctgcaga agcagaacct
     2521 aatttaggat cgctcaatgg cttgatgaca ttaccttgga caggtgcatc cactacatct
     2581 tgcgggatac tgataaagct cgcgcctttt tttgaactct tagccatacg ataagcattg
     2641 gcaatcactt ctgaaattgt ttctggatct tggatttctg cactgtattt tgtgattggc
     2701 tggaacagtg ctgcattatc catgctttga tgggtcaact tcaatagatc gcttcgtttt
     2761 acttgtccgg caattgctaa aactgggtca ccttctgctg tcgctgttac caaacctgta
     2821 gctaagttac tagcaccagg accacttgtt gcgataacta ctcctggctc tcctgtaata
     2881 cgtccgattg cttgtgccat aaatgcagca ttttgttcgt gacgagtcac gattaattca
     2941 ggaccttgat cctctaattc attaaataca ccatcgattt ttgcacctgg tataccgaat
     3001 acataatcaa catgatgatt aatcaaactt tctactacag atttcgaacc ttgtttcatt
     3061 ttttcagcca ttgaacatcg tcctttttat gctttatttt taattattag ttagaaataa
     3121 tcacaaataa gtgatacagt ttctaaacct gtttcataca aaagtatagc acgttttatt
     3181 tcatttgttt agccatatgt ttcaaaaaat gaaaaagaat gacagttttt acataaaaaa
     3241 aactgtcatt cttaattgaa agtttcaatt tataaagtct acagatccat agttctcatc
     3301 aatttacgat tgttgaagta aatcaaacca aatacgatag aaggacctag tagaatacac
     3361 cattggggct ttacgaaaaa gaagagaaac aaacttgtca atagtaaaaa atctagtaaa
     3421 ctcacatacc attttttcaa cgtatagtag aacgctatcc gtagaacttg atttggtttc
     3481 gacgctggat ttctcacctg aaaataccag cagttggtgc taaaggaaac tgataaacaa
     3541 gcaagtaaaa caaacaacgg aatcaaccat tttccataag aaaaacgtat aaaaaagaaa
     3601 caatcaaaaa tcattatcac actaactatc caagcaaata gccaataaaa aaatcccttt
     3661 agccaaaatt ggcgaaacat ctttatgaac aaacgtactg gtgaaaaact attttctttt
     3721 gctaattctt ctgtcatacc tatcatcacg atgcaacctg gaccaataaa aagtaaagaa
     3781 aaaataaagg aaaagacatt ccttccatct atcgacaaaa ggaacgcagc tgctatatac
     3841 aagatattga cacaccaaaa acaaaggcta cttgccaatg attgatacaa ccaataaaag
     3901 aattccatat aaacattttt gtcaaaacgc tgctctttca taatcacctc ctattccttc
     3961 attcctgatg aagcgatccc ttctacaaaa tatttttgca tcgctaaaaa tataaaagca
     4021 acaggaagaa cagaaacaac tgaggcagcc atgattaatg cgtactctgc atcaaatagc
     4081 ccaataaaca ttttcaaccc taactggatt gttttatttt ccgtagaaga caaataaatg
     4141 aatggcccca tataatcgtt ccatgtatta acgaatgtga tgatcgtcaa actggcaatc
     4201 gccggttttg taagaggtaa aatgatcttg cgatagattc cccactctga taagccatcg
     4261 atccgcgcac tctcacataa agaatctggg atactactgt aatattgctt caacaagaac
     4321 acaccaaaag cattgaacgc ttgtaatagc accaacgacc acaacgtatc tgttaaaccc
     4381 acttttctca tcataatgaa ctgcgggatc atataagact gccaagggac agcaatcgtg
     4441 ccgatatatg ctaaaaataa aatatcgcgc cctttaaaat ttagtttaga aaacccgtaa
     4501 gcagcaaaac tagaagtaaa caactgaatc aacgtgatca caacgcttaa aaaagctgta
     4561 ttcttaaaaa atgtcactaa aggaatacga gaccaaataa ccagataatt ctctaaatgc
     4621 caagttttag ggatccattg aatcggaatc gtgaacactt cattatttgc tttgaatgaa
     4681 gcagaaacca tccataaaaa aggaatgatc gtcaataaag ctaatacaat cagcagaatc
     4741 atacccaacc attgaataaa gggcatgttt tttgaacgaa tttttgcttt ttttctttca
     4801 tttcgaacac ctccaaagtt ataatcttca tttctttgta atgattacaa tccagaacaa
     4861 cggttattat agctgattca tttccgttct tcttattttt gtaagcgctt atttaacaag
     4921 ttatgtaaat ctctttattt ttttgattta aaaatgaaaa aatacaaaag caagactgtc
     4981 atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac tgttcaaaag
     5041 cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga ttctttttcc
     5101 agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc actcgctttg
     5161 aatgatgact tacagcacct tctatgacta aagcagcacc tacctgattc tcaattagcg
     5221 caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc ttttttatgc
     5281 ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa agaggtacat
     5341 tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt tctaaaatga
     5401 gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct gtatctactg
     5461 gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg atatcatcac
     5521 ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac ttttctagta
     5581 atagtgccaa tgtattcgaa ctatcagctt ccacacagcc gatcgtaaat cggccgctga
     5641 ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa tctaaaattt
     5701 cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt gcggttttcg
     5761 cgtacaaaca gttctgttcc tagttactct tctaactcct tcagttgtcg gcttaaggtt
     5821 ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc tgcaattgtc
     5881 caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt tacttgatac
     5941 attccatttt atttctagat aacacacgat catatttctt gatttttcct tgtaaacgtt
     6001 ccaaacattc ttgcatttca gcaatttttt tcgtcaaaag ttcactttct tcaatcaaaa
     6061 gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata tattcaatca
     6121 aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc cattttaggt
     6181 cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga atcaacccga
     6241 ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg acttgtttga
     6301 tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta ttttatttat
     6361 tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta agctggatta
     6421 tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt tattcttttt
     6481 tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa atgacaacgt
     6541 atcagcgaaa ggtattagta tcgacatcgg cgggaattgc tttagagaat atggatatta
     6601 tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt ggtacgcaag
     6661 cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt ttttttggca
     6721 tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt ctgttttcga
     6781 ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc tttcgcttta
     6841 tcgcaggtgt aggagcggga ggagaatatg gggcgtgtat gtccttgatt tccgaaagtt
     6901 tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg caggtcggtg
     6961 cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg aagatgttgt
     7021 atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc ttgactgaac
     7081 caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc agactgtttg
     7141 aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact gtccaaattg
     7201 ccggctattt tgggttaatg aattggctac cttcaattat gcaaaatcga ttagggcttt
     7261 cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct ttggggatga
     7321 tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct ttgtttttaa
     7381 tttgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg agtttggtag
     7441 cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat ggcgcagtga
     7501 ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcgtt atgaaccttg
     7561 gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat cattatagtt
     7621 taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg gtcttgacaa
     7681 ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg agtctgtaaa
     7741 tgacaagaaa gaggtgtgga aaaagcttgc atctgcggta ataagaccag ccaaatcaaa
     7801 aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc agctttttca
     7861 actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat ttcaaagtac
     7921 tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg agcgtctgca
     7981 taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg ataattatat
     8041 gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg cttttccata
     8101 gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattg ttggaacaat
     8161 tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa ttcgttttgt
     8221 agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat tttaattttc
     8281 ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc tactttacta
     8341 tactacatac aagaaaaaaa cggatacaaa tatcatattg tcacaccgtc aatagtgcat
     8401 aaacctgtta ttatttctct ttttttccta agtattctat tgatatgaat catttaatcg
     8461 tttattatat aaatgtcaat aatctaataa taaaataacg tacatatttt attattgcat
     8521 acttaataac agaactatag caaaaaatcc cgtgtatcaa cacgggatta gattaagttt
     8581 cctcaatcac tattcataac acaattatta taacaagtaa gtaaacaatt gaaaaggaca
     8641 tttattgaaa acggttgttt tttacttaag gaggaaattt agtgaaaaaa ttagttgcgt
     8701 tgtttttggt cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct actactcaaa
     8761 gcacaagttc agctcaggct tccagcagca gtaccacgat ctccgaaagt acggcagatt
     8821 catcgaaaga acaaattgaa aagaaaaaag aagaagagaa acaaaaagcg gaaaaagctg
     8881 cactagaaaa aaaagtgaca gaagcggaat ctgctttgtc acaagctgaa gcaaatccta
     8941 caaaagaaaa ctatgcaact gcttcagcag ctatccaagc gctacctgaa aacaaacaag
     9001 agttgacaag ccgtcttgct gcaattgatt ccacaatcaa agcaaaagaa gcagaagaag
     9061 aacggaaaaa gcaagaagaa attgcacgac aacaagcaga acaagcgcaa caagaagcaa
     9121 tcgcacagca acaagcagaa caggcgaggc aagaagcaat cgcacagcaa caagcagaac
     9181 aggcgcgaca gcaagcagca gctgaacagg cttctcagca ggcacaagcc gcagctcctg
     9241 agcaaaataa tgaacaaact gtctatgtca caccgaccgg ttcaaaatac catacacata
     9301 aatgtggaaa tggtacgtat acccctgcca ctttaagcga agctcaagct cgtggtttaa
     9361 ccccttgctc caaatgttat ggaaactaat tcaaaagtta tactataata ataaaattct
     9421 tctgaaacat aaaaaaatag aagctggcgt tccagatagt aaaccaaaaa tagcagttta
     9481 cacgtccatt tcgtcagctt ctctcttttt tcatttagtc tgttccctct gcttttttgg
     9541 acatcttcat tcattctttc tcaatcaaat agtacacctc tttttctttt cgcagtttat
     9601 atcttccttt tttcaatttc attgaaaact gtttcgaatg tttatgtttt acatggattt
     9661 taagcaggag ctcctcatgt tgcaagcgat agttcctgcc ttttaaatca gaaaatctca
     9721 ctagtccgta ttcatcagaa gctttcacaa atggaacttt ttctctagta atatagtgtt
     9781 tgccgttgag cgtttttaca gaaaagacca ttccttcgat tggtcgtccg tttgtgttct
     9841 ttacataaaa agataaagga tagtttcgct tagcgatggc caaagaatct aaataagcga
     9901 tgagtagcca aactaagccg attgatccaa gtagtatacc gataaaccaa ataaaaaatt
     9961 tccatttttt ccattgatca attccttgga tcgctttttt tgcctctttt gcttgataag
    10021 gaatacgatg ccccgtcaca agcagtcgat gtgtattgac catataagga gtgcaggtaa
    10081 gcaaagtcac gagatctctg cctttttgga tacccaaaga atctacttcg tcaggtaaga
    10141 ccacttggat tttttctact tgatacgcca atgtctttcc attgatctgg atgaaaaact
    10201 ggtctccttt tttcaactta ggcaaatctg taaataattt tgcttctggg agtccacgat
    10261 gcccagaaag aacggcatgg gtgtttttcc cgccagtcgg ataagaactt ccttccaata
    10321 atgacgttcc tttttgcaaa aatatttccg ttgtctgatc gaatataggt aagctgacat
    10381 tgatttttgg gatttcgatg actccaagca tatgttccat aaaaaaggca ttactaggta
    10441 aatcttttaa tgcttttgca tcaactgctt ggttgaaact gcctatccct ggtgatacgt
    10501 tttgctctgc caactgttgg tttttctttg tcattttttc ctgctgtttt tttatttcct
    10561 ctgaattctt attcgatgct tgcttttgat aatggatgat catttgctgg gcaagaaaat
    10621 cattcagtga atcttctaca aaaggataag aaaagatccc gatcccaacg actaaaatga
    10681 ttgccatgaa gatgtccagt attcttattc tgcttttatt catcatgtcc acctattttc
    10741 taatatgtat atgaagaggt tgtgaaatca gcgccttgac ctgagtatta gcgaagcaaa
    10801 cggcaaaata gttcctccta ttttgccgtt tgttgagcga ataccaagag accgaccact
    10861 tgtcacaacc tttctcccca tttgaatcac tatcgtttat ttatcgtatg atttttgttt
    10921 ccaatagttt aagcttctgt ttcttgatgg cgttttttga aatagatcac tgctcctcca
    10981 actaaagcag tacctaccaa gatgaatacg atgatcccca taccaccagt tgaaggcaag
    11041 attcctttag gttggttatc gatcgttgtt tcatcagaag ttgttggatc atcatctact
    11101 gtaaattcag tgtacgcagc ttcattctta ggtaatgcgt agccttctgg tgcttttgtt
    11161 tcttgaaggc agtatgcccc ttgtttcagc ccttcgattg aaaatttccc ttctgaatca
    11221 gaaatcaatt tcacttcttc ccagtcagaa cctgtgactt catctgacca agcatatggg
    11281 tattctcctg aggcaaggtt tgctattttt ttcattaatt taacagttgg atcttttgct
    11341 cttagaatag aaaattctgc atcggctaat ttttcttgcg tgttcacatc ttgtttgatg
    11401 aagtggaaat caccgtagtt ttggacattt tctacagtaa tcgtgttatc cgtagttgta
    11461 ttttcccgat tgactacacc gtttggattc ccaagattgg cttcatctgc gccatggatg
    11521 ataccggttg tttgttgcaa ttttccgttt tttactataa aagtagtttc tttttgtgga
    11581 ttggttaatt gataaccatc tggtgctttt gtttcaacta aagtataatt gccgtctatt
    11641 aatccattga cactgatgga tccttcagta tcagttgtga atgtcaacgc tgactcagca
    11701 ggatcataga gagcgacacc agtgttttca tcaaaaccgg tcacgaattg attctctgaa
    11761 tttttgatct ggaattgcgc ccctgctaac ttatctaccc ctgaaaagtt cccagctttc
    11821 tggattttga tattgctttc tttgatgaca tttttagcat ataggtgaat cgtgtctaaa
    11881 ggtttgtcag ggtctagggt aagcttaaca cgatatttgg cgacgtttcg acaccgggct
    11941 ttggtgtttc tacaaagacg tatacagcat ctttcccatc agatttacct ggaagagtca
    12001 ggttagctaa tccttcacca gaagtcgtta cacttgcgaa gggtgtagcg tctgctggaa
    12061 ctgctgtgtc tttacttgga tagtacgtag cagtgatgtc atatacgttg aattcaacgc
    12121 cgttcaacgg ttcgctattc ggaaatgtca attccgttcc gttgtttatt tgattttctg
    12181 gtgctgaagt gaatttcaat ttatgcaaat taaccgtaac attttctggt gtaggtgata
    12241 cggctgcaga aacctctgca gttcctaggc cgagaacgaa aggaagcagc atgatagcag
    12301 ttcctaaaaa cgtccatatt tttgttttga tgtttttcat tttatttccc tcctattgtt
    12361 tcctttttgt tgttttttat aaaaccaagc tgtccctgtt gaaaggacta gcaataaccc
    12421 taaaattgag aaacctccaa gcatctcttc gcttgttttt ggaaagcttc ctccattttt
    12481 ttcatttgat ggaccatttg tctgcatctg aactggcgtt tttccattat tcgtcggatt
    12541 tgcttttggt tcgttgatga tttccaatgg tataggatga agcggatcat aactgccttt
    12601 ttcaacttca aagagaatgg ttgattgact caatatatat cctttaggcg gtttaatctc
    12661 cactagttcg taacttccaa aagacaatcc atggacagaa aatgttccat ctttactgga
    12721 agtaaaaatc gttgctcttg aatcatcttt ggctacccaa tgatttatcc cgttttcttg
    12781 cgctaagtat tggtcatttt ttttgactag aaattgagca tctgctagtt tttgtttcgt
    12841 ttgccgatcc actttgacaa attgtttgcc tcctgtttgg atggcaagat gtgtttctac
    12901 tgtttgattg tcagtcgtca aacgtacatc gttatcaaat gtcgtttttt cactgttttc
    12961 tttcaatgtc atttggtaac ggatcgttaa tttttttcca gcaaacgaac taagatcttt
    13021 tccagaatct gataagacaa aaccatgttt tattttttgt gtgtgaaatg tatgaatctt
    13081 gtctccacca atcaaaaaat cgatcgtttc tggttctagc cataactgag gatcagctgt
    13141 atctttgatc gtataggttc ctatctcgtt gatgttctca ggaattgtta cggtgatgag
    13201 ataagggact ttatccccat ctgcaaaatc tgtctgctta ttctctactg tttttgttaa
    13261 tgtgagtgca gcaggtattt tttgttcgct ctttggatac aagtggatgg tcgtcaattt
    13321 atgattagat gaatctgtca caggaagcac aacgacaaat ggtgctgatt tttcttttac
    13381 ttaatctgaa gtctttattt ccgtaaatcg gtaaacagcg tcacgacctt gctcatcttt
    13441 gtccgatgca ctaaaagaag caatgccctc ttcctcgtct actgtttttg tcacattttc
    13501 tgccagtatt ttttcactat cacttttttg tgctagtttt ctttgtgcgt cttccacact
    13561 acttccttct gaccgcagct tgtaaaattc atccgagaca tcatatatag cgaaagtaac
    13621 gccgttcaac ccttttactc cttgaaagtt atccgggata tttattccgt cgttttgact
    13681 gatacttggc atcttatcgc tttcgaacat tcgattatgc aaagtaaatg taataggtga
    13741 ttctctagct gacacagtcg gagaacctat aaaaaagacc agtaggataa aaaagagaac
    13801 agcaaatata tccacacgta cattttttgt ttttttcatc gctgttcacc tgcttttttc
    13861 ttacgatgga taaaataaaa gaagccagaa aaaataaaga tcagtcctaa aaagacaaag
    13921 ggcagtagac ctatcccacc tgtcgaagga agcaagagag tcggattgat cgtcaactta
    13981 gcggtatctg ttgcgataat cgtataatta ttgtttacac ttccttcgcc taaactaatc
    14041 gttactattt gtgatccaat atctgtccgg ctgttaactg ttagggtata atccaatgat
    14101 tcgcctaaga caacaccggt aacgtggaga tttccaaccg aaatcgagtg actttgggca
    14161 tcataattaa aggcagcgtc atcgacagta atcactgcgt tagctttata gatggtataa
    14221 gttcctgatg tgttggctat atcagtaaat gacaacttgg gatttgctgc tttgactttg
    14281 tctattccgc tattcgttag tttaattgta tagctgccag cattaaccat attttttgct
    14341 gattgtgcat ctgctgtgta atccatactg ctaagtgcca cttgtactgg agtaagcgaa
    14401 ctagtgacac cctctgctag cttgacatgg attgacggga cacctgcttt tccatcatat
    14461 gtcttactgc cactgagctc taccgcaccg ctgatttgct taatcgtata gacatcagat
    14521 gtctgcacga tagtaaataa atagtttgtt ctggcagcta gtttagctag tccgcttttt
    14581 gtcaattgcc aagacgtata ggttccaatt tcagtaggcg cagttacttc aactcccttt
    14641 tcattcatcg ctttgaaatc agttggattc catgtgattt gatcaatact gagatcattg
    14701 tttaaaaggg atgttgattc tgttttatca ttaaaggcat atttaacgat cggtacaaca
    14761 gaaagaccat catagtactt actgccattt gcattgattg atactgttgc atttgtctta
    14821 taatatagga tgacttcaga aatctgtttt ccatttgcgt cctttacgat tacatttcca
    14881 tttgtatcta aactagctaa aacactcatt gctgattggt tctttgtttc tatcgctgaa
    14941 acagctgtaa aggtatatcc gttgatagta ggcgtattag tcgtcaggtc ttgtgcagca
    15001 cttccgtaag taagattcat tattttagaa ggagcaatca tctgtccaaa atagtcttga
    15061 tacttaatag tgattggtgt ttgtttgcct gtataggtca ccgtatcgac gttactttct
    15121 agggctgtga aagaaccgtt tgtttgtgca aaagaatagc ccgttactgc tgttggagtg
    15181 ataaccactt tgtcacccac atggattcca gataatgtag tcccgtacgt tccgattttt
    15241 gctgctccac cattgactgt ataagaaaaa gttcctgtcc gattcgcatt gacctgaacc
    15301 gttgctgctt gataggttgg aaaatttgaa acatttccct taccatcaac gatctgccca
    15361 tttttgatcg agtaccccgt gaatggaata tcatttgcta actgtatacc gattgaaaaa
    15421 gtactcatac ctcctccggc aggaagccct ccccatgttg agggtgtaaa agaataactt
    15481 ttatccgcat tgggtgtaac tgaatcgatc gtatttccag cggtagccac ccccatatag
    15541 atactttttg ggaatgtgat agtagtacca tttactgtgg agccaataaa cagattatgg
    15601 atatctggac ggttatcaat aaaagcgatt gctttggtta cgccttgtaa cgcagtttta
    15661 tccgcactca tcgatccgtt aaccgcaaca atgactccat ctttggcata tatcactgta
    15721 tatccatctg gtatggtatg tccgttgaaa ttcgcagtgg ccgcaacagt actaaatgtc
    15781 tggcttctac ctgtttctga ataagctttc tcccctgctg cttttgctgc tgtgatttct
    15841 ttttctgtcg gatcaatcag ttgagtcgag gatgtggttg aggttgtctc gttgttgcta
    15901 gactgattgg tgctttctat ggactgtatt tcagaatagg gtggactatt tgtctcctct
    15961 gtcgtttggt tagcaaaatt tgacgtttct gtataaacgt ttacataaat acttgataag
    16021 ctccctaaga aagtaaaaat caagatgatc gaaatcataa tgggatgtaa gtattttctt
    16081 ttagatacag ctttgagtct agaaacaaaa tgtttttgac atatacgttg gattttattt
    16141 tgagttttct taatcatttt catccctcct ctcaataaaa aatcatcaaa taatccttta
    16201 cacgttattc taaatttaat ttttattatt ttattgatgt atagaaagtt tggggctttt
    16261 tttctctttt tat
//
LOCUS       DARBEG010000060        16003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000060 DARBEG010000000
VERSION     DARBEG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.061"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..894
                     /locus_tag="SCK20_001933"
     CDS             226..894
                     /locus_tag="SCK20_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG1145977.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="SCK20_001934"
     CDS             complement(1094..1480)
                     /locus_tag="SCK20_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145978.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="SCK20_001935"
     CDS             complement(1452..2834)
                     /locus_tag="SCK20_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG1145979.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="SCK20_001936"
     CDS             complement(2990..4177)
                     /locus_tag="SCK20_001936"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG1145980.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="SCK20_001937"
     CDS             complement(4184..5215)
                     /locus_tag="SCK20_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1145981.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="SCK20_001938"
     CDS             complement(5231..6685)
                     /locus_tag="SCK20_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1145982.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="SCK20_001939"
     CDS             6860..7765
                     /locus_tag="SCK20_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1145983.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="SCK20_001940"
     CDS             7856..8533
                     /locus_tag="SCK20_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1145984.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="SCK20_001941"
     CDS             8530..8700
                     /locus_tag="SCK20_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145985.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="SCK20_001942"
     CDS             8814..9710
                     /locus_tag="SCK20_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1145986.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="SCK20_001943"
     CDS             complement(9765..11135)
                     /locus_tag="SCK20_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1145987.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="SCK20_001944"
     CDS             11373..12119
                     /locus_tag="SCK20_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1145988.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="SCK20_001945"
     CDS             12116..12955
                     /locus_tag="SCK20_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1145989.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="SCK20_001946"
     CDS             12956..13906
                     /locus_tag="SCK20_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1145990.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="SCK20_001947"
     CDS             14047..14799
                     /locus_tag="SCK20_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1145991.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="SCK20_001948"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="SCK20_001948"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG1145992.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5193 a         3004 c         3222 g         4584 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt cca
//
LOCUS       DARBEG010000061        15952 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000061 DARBEG010000000
VERSION     DARBEG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.062"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(388..810)
                     /locus_tag="SCK20_001949"
     CDS             complement(388..810)
                     /locus_tag="SCK20_001949"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG1145993.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(824..2230)
                     /gene="atpD"
                     /locus_tag="SCK20_001950"
     CDS             complement(824..2230)
                     /gene="atpD"
                     /locus_tag="SCK20_001950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG1145994.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2253..3155)
                     /locus_tag="SCK20_001951"
     CDS             complement(2253..3155)
                     /locus_tag="SCK20_001951"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG1145995.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3169..4725)
                     /locus_tag="SCK20_001952"
     CDS             complement(3169..4725)
                     /locus_tag="SCK20_001952"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG1145996.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4748..5290)
                     /locus_tag="SCK20_001953"
     CDS             complement(4748..5290)
                     /locus_tag="SCK20_001953"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG1145997.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5277..5801)
                     /gene="atpF"
                     /locus_tag="SCK20_001954"
     CDS             complement(5277..5801)
                     /gene="atpF"
                     /locus_tag="SCK20_001954"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG1145998.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5892..6107)
                     /gene="atpE"
                     /locus_tag="SCK20_001955"
     CDS             complement(5892..6107)
                     /gene="atpE"
                     /locus_tag="SCK20_001955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG1145999.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6160..6879)
                     /gene="atpB"
                     /locus_tag="SCK20_001956"
     CDS             complement(6160..6879)
                     /gene="atpB"
                     /locus_tag="SCK20_001956"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG1146000.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7181..10024)
                     /gene="rnr"
                     /locus_tag="SCK20_001957"
                     /pseudo
     CDS             complement(7181..10024)
                     /gene="rnr"
                     /locus_tag="SCK20_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10027..10782)
                     /locus_tag="SCK20_001958"
     CDS             complement(10027..10782)
                     /locus_tag="SCK20_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1146001.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10885..11121)
                     /gene="secG"
                     /locus_tag="SCK20_001959"
     CDS             complement(10885..11121)
                     /gene="secG"
                     /locus_tag="SCK20_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG1146002.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11239..11517)
                     /locus_tag="SCK20_001960"
     CDS             complement(11239..11517)
                     /locus_tag="SCK20_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1146003.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11630..12598)
                     /locus_tag="SCK20_001961"
     CDS             complement(11630..12598)
                     /locus_tag="SCK20_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1146004.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12813..12857
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12881..13855
                     /locus_tag="SCK20_001962"
     CDS             12881..13855
                     /locus_tag="SCK20_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG1146005.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13860..14435
                     /locus_tag="SCK20_001963"
     CDS             13860..14435
                     /locus_tag="SCK20_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1146006.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14502..15641)
                     /locus_tag="SCK20_001964"
     CDS             complement(14502..15641)
                     /locus_tag="SCK20_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG1146007.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5296 t
ORIGIN      
        1 tctgagccag aatgtggttc attctgactc agatttgatt ttgcacaact catcagtcct
       61 tattgacaaa gagcccaaaa gtcttgtcgc agtcccttat tccaaataaa cggtgttcaa
      121 tcatctgacc ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttccattt
      181 gtttctctaa tactcaggac aaaacgctga tttcacaacc tccgatatac ggtgttccag
      241 aagtagcttc ttcgaaaaat aagctgagat ttcacaaaaa tttgagaagc aattttcgta
      301 aattccttct tatgtcttga agctgaccac ttctgtcata acctcttttt actgttctta
      361 aacacggata aacatccgca tctcatttta tgagtgctta gatactttga tacgattgat
      421 tgctcggtgc aacgcaacag ttgcccgttt caattcgttc gtatcttgtt ttgcttttgc
      481 ttcttcgatt tggcgttctg cgcgttgttt tgcacgttcc gcacgagata catcgatatc
      541 acgttcacgt tccgcactat cagcaacgat tgaaacaaca ttatcacgaa cttccatgat
      601 tccgccatta acagcgatcc aatcgacgtg ggtatctgaa tctgttcgtt ttacacgaac
      661 ttcatcaatc gtcaatggaa cgatgattgg tgcatgttta ggcaaaatcc caagctcacc
      721 agctgtcgta cgtgcaacga cgatagctgc atggtgatca tagaccaaac cggcaggagt
      781 taccacattt actgttaaat attgatccat ggggcacccc tttctagtag cctagtgttt
      841 tcgctttttc tacgacgtct tcgattcggc caacacttcg gaaagcttct tcaggaagat
      901 catcgtattt gccttccaag atttcgcgga atcctttcac tgtttcttct acagggacat
      961 atgaacctgg aagaccagta aattgttcag ccacgttaaa gttttgtgac aagaagaatt
     1021 ggactctgcg ggcacgagaa actaacactt tttcttgatc ggataattca tccataccta
     1081 agatagcaat gatatcttgt aattcacgat atcgttgcaa tacgtgttgg acttctgttg
     1141 ctactttata atgttcttct cccacgattt caggtgctaa agcacttgaa gaagaagcca
     1201 atggatctac cgctggataa atcccttgtt ctgttaattt acgttccaag ttggttgtcg
     1261 catccaaatg ggcgaatgct gttgccggcg ccgggtcagt gtagtcatcg gctggcacat
     1321 agattgcttg gatcgatgtg atcgaacctt ttttcgtaga tgtgatacgt tcttgcaatt
     1381 gacccatttc tgttgccaat gttggttgat aaccaaccgc agaaggcatc cgtcctaaca
     1441 aggctgatac ttcagatcca gcttgagtga aacggaaaat gttgtcgata aacaacagta
     1501 catcttgacc ttctacatca cggaaatatt ccgcaatcgt caaaccagtc aaagcaacac
     1561 gcatacgtgc acctggtggt tcgttcattt gtccgaacac catggctgtt ttttcgataa
     1621 ctcctgaatc tttcatttca taatacaagt cattaccttc acgagtacgt tcacctacac
     1681 cggtaaatac agaaatacca ccatgttctt gggcgatatt atggatcagt tcttggatca
     1741 gtacggtttt accaacaccg gcaccaccga atagtccgac tttcccacct tttaaatatg
     1801 gggcaagcaa atcgataact ttgatccctg tttccaaaat ttctgtactt gtacttaatt
     1861 cgtcaaaggc tggtgctttt ttatgaattt cgcttctttc cgcatcttct gggaaaggtg
     1921 tttctaaatc gatcgtatct cctagtacgt tgaatacgcg acctaatgtt tctttaccta
     1981 caggaacaga gattgctttg cctgtatcaa tgacttccat tcctctttgt aaaccatctg
     2041 ttgattccat cgcaatggtc cggatcactc cgtcccctaa ttctaacgct gcttcaagaa
     2101 caacttttga tttattttcg tcatttttat aaacaactaa tgcgttgttg atatctggta
     2161 aggattggtc taaagaaaat tccacgtcga caacgggacc gattacttga acaatcttgc
     2221 ctgaactcat cttttttcct ccaatctcaa tactattcta gtgccgaagc tccggcaaca
     2281 atttcggtaa tttcttgggt aatagcccct tgtcttgcac ggttgtaaga aaccgtcagc
     2341 tcatcaatga tattagcagc attatcagtt gctgttttca ttgctgtcat accagcagca
     2401 tgttctgctg ttttggcgtc aacgatcgca ccgtagatca aactttcagc atactgaggc
     2461 aataactgag acaagatttc ttcttttgaa ggttcaaaaa tatattcttg ttcgaaagta
     2521 gttgcttctt ctggatctaa gtctgagatt ggcagcattt tttctacacg aaactgacta
     2581 gttagcgaat tgatatgatg gttataacat acgtataatt catcgaatac ttcgttttgg
     2641 tacatagttg ttgccatatt tacgattttg cggacttcat caaagcttgg ctgatcagat
     2701 agattgcgca gctcatatgc tagattgata ccgcgtgctt taaagaagtc tgcaccagtt
     2761 ccgccaattg cgatcatcac gtaatcatcg ggtgattggt ggtcttcttc taaaatagac
     2821 atcgtttgtt tcaatatcga gctgttatat ccaccgacta aaccgccatc tgcagtgatg
     2881 acgatataac ccgttttctt aacaggacgg ctgagcaaca tgctgttgta attgatatcc
     2941 cctctaggat tttctgaggc aatatcattt aactgagtag cagtcaaatg cgtaacgatt
     3001 tctcttactt ttgttgcata aacctgaaac ttctgtgaag aagcttcaga ttttgtcaac
     3061 ttagaagcag acaccatctg cattgcccgc gtgatttggc tcgttttttt cgttgaagcg
     3121 atacgtgtct tgatttcatt taatgaagca cccattcatc tcacctcact atgcattttg
     3181 aatggatttc aatttatctt ctgtagagga attatttcct tttgtagcta agaacatgtc
     3241 tttatattcc tgaatcgcac tgttcaacgc ttcttcttct ggaagatctt ttgttgtacg
     3301 gattgtttca aagatatctg aatgattggt atccagatat tcgaataatt catgttcgaa
     3361 gtctaaaata ctgtctacag ggatactatc taagaatcca tgtgtcaaag catacaaaat
     3421 cactacttgt ttttcaacag ctagcggtgc atgcaatttt tgtttcaaga tttctactgt
     3481 acggcgtcca cgatttagtt ttgcttgtgt tgctgcatcc aagtcagaac cgaattgtgt
     3541 aaatgcttct aattcacggt aacttgctaa gtctagacgt aaagtcccag caactttttt
     3601 cattgctttg atttgcgcag aaccccctac acgagataca gatagtccgg catcaaccgc
     3661 tggacgagta cctgcgtaga acaagtcgct ttctaagaag atttgtccat cagtgatcga
     3721 aataacgttt gttgggatat aagcagagat atctccagct tgtgtttcaa caaatggtaa
     3781 ggcagtcatt gaaccgccac ctaaatcatc acttaatttt gctgcacgtt ccaataagcg
     3841 tgagtgtaag tagaaaacat cccctggata agcttcacga cctggtggac ggcgaagcag
     3901 caaggaaagt tcacgataag caacagcttg ttttgaaaga tcatcaaaaa tgatcaagac
     3961 atgtttcccg ttgtacatga actcttcacc cattgcagta ccagcatatg gtgcgatata
     4021 aagcaacggc gctggttgag acgcaccggc attcacaacg attgtgtaat ccattgcgcc
     4081 atattttttc aatgtttcaa cttgtgtacg aactgtagaa tctttttgtc cgattgctac
     4141 atagatacag atcatatctt gacctttttg gttgatgatc gtatcgatcg caatggaagt
     4201 tttccctgtt ttacggtcac cgataactaa ttcacgttgg ccgcgtccga ttggtacaag
     4261 agcgtcgatt gcttttagac ctgtttgcat tggttcgtta acagatttac gttgcataac
     4321 gcctggtgcc attgcttcta ctggacgagc tttgtcagta acgatttcac ctagtccgtc
     4381 aattggctga cctagcggat taactacccg tccaatcaag gcctctccaa ctggaacttc
     4441 catgatcttt cctgttcgtt ttactttatc tccttcacga atggactcga aatcgccaag
     4501 gataatgata cctacatcat ttgattctaa gttttgcgcc attccgtatg agccatttga
     4561 aaattcaagc aactcaccac tcatcgcgtt ttctaaacca tgtgcacgag cgatcccgtc
     4621 acctacataa gttacggtac cgatttcttc gactgaaagc acgttttgat aattttcaat
     4681 ttgttcttta atcaaggcac taatttcttc tgctttgatg gccattcgat tcacctacct
     4741 ctttgtacta tctatttaat tgattacgca tattttctaa ttgcgtgcga atgcttccgt
     4801 cgattactcg atggttagct tcaacaatcg cgccaccaat aatagaagaa tcaacgattt
     4861 gttttaattc gactgattga tagttcattg tttttgcaac attcttttct aattgtattc
     4921 gttgttcgtc tgaaagtgga actgctgttg tgacacttcc caacagcaaa ccttgatgtt
     4981 catcatatcg acgttcatat tcttcgatca tgaacaaaag atcatccatt cggttatacg
     5041 cataaacaac ttctaaaaag tttttaacga ttccatcata gccactaaca agcttgtcca
     5101 tgatttctcg tttttcatga ggttctagac gaacatcact taacatatta cctaatccag
     5161 gtacttgttc atagatttga cgtaagctta cgagatcttg ataaatttct tctgctttat
     5221 ttgaatcgat cgccagttca aataaagctt tcccgtaacg tcttcctact gtatatttat
     5281 ctagtttcat ttgaagaacc tagaccttcg atatattgat tgattaatga ttcgtgcatt
     5341 tctggagata attctttgtt caagattttt tccgcgattt gaagagatag ttctgcaaca
     5401 tcgtctttta cagagttcag cgctgaatca cgttctacag tgatatcagc ttgggcttta
     5461 ctttttagac gagctgcttc ttcttgagca tccttcaaaa tattttggcg gcttaattct
     5521 ccactttctt tcgcattttt gatgatatca gctgcatcag aacgagaggc taacaattgt
     5581 tgttcgcgtt cttgttccat tttcgctgag ttgatgcgag attgttctgc agaatccaaa
     5641 tcattggcga tcttgtcttc acgttttttc aaaatatcgc tgattggtcc ccaagcaaaa
     5701 tgcttcaaga gagccagtag tatcaaaaat gagccgctga caacgatgac atttcctaac
     5761 atcgcattgc caacttctgc aattgccaat tgattcagca taggcgagcc ttcctttctt
     5821 tgaagtactt ctagttttca tgtaaaaaag cagtaacaaa ccaaattgtt tctgccttaa
     5881 tactgattca attaaacacc aaaaactaga atcaaggcga caactacccc taagatagga
     5941 acagcttcga ctagagctac cccgataaac attgttgtac ggaattgacc agacatttca
     6001 ggttggcgag ccattgattc gattgtttta gagataactt gaccgttacc ataaccggct
     6061 ccgattgctg ctcccatgat tgcgattgct gctgcgatat aattcataat tgttttcctc
     6121 ctaaattaga taaatagtag tttgttttta aaacgaatat tattcttcca cttcaatctt
     6181 atggctcata taaaccatag ataaagtaac gaagacaaat gcttggatgc ttccgataaa
     6241 caaggagaat gcgatccaga ccatctctaa tggaattgct aacggcaatg agaaccagcc
     6301 taagcttatt accatttttg caatcaaggt tagcaaaact tcccccgcat aaatattccc
     6361 ataaagacgt aaagcaagtg ttaataagtt tgtaaattct tcaatcagtt tcatcggaaa
     6421 cataaaacta tatggtttca agaacgaatt tacgaaatat cctttgaagc ctaacttttt
     6481 caccccaaat aagtgagtaa gtaagatcat tataaatgcc aaagttaaag taacaaacgg
     6541 gtctgctgtc gggcttttcc aaagtgttgt ttcgttaggt aaaacgattt ttgttactaa
     6601 accgatgttg tttgcaacaa aaacaaataa aaacaatgta aatgccaaca aatggaaatt
     6661 gctcaattct tttctaggca gattatctgt tacgattcct cgactaaaat caatgaccca
     6721 ttctatcgca ttttgtttgc ctgttggttt caacttaaga tttcttgtaa aaaagtaagc
     6781 taagaagaaa acaatcaaac aggtcagtaa caccattata caaactgtac catcaaacca
     6841 aattggtcca atgtggaacg ttagcgagcg ttcatccaat ccatttcacc tcttttcccc
     6901 gaaattcaca taaaatcaat tcatgtgcgt atctggtttt ttccaatcgg tttctaacca
     6961 cacggtatca taccaccatg aaacagaaaa ttcaaaacat tttcatgaaa attagacact
     7021 tttttatttc agtcaaaaac gaagcgaatt tttctttata cacggtgctc ctgatctaag
     7081 aaaaaaccta tgataatcat gttttatcag attaaatgct aacaaatttt atcattaagt
     7141 aaaagcaaaa gcaccgaata tgcgaaaaag gataatgaaa ttatctagag aaatttttta
     7201 atgttcgatc aatttgtcga tcgatatctt ttcgtttcaa atcttcccgt ttatcataag
     7261 attttttccc tttcgccaga ccaatcaata ccttggcata gccgttacga atatagactt
     7321 ttaaaggaac aacagtgatt ccagtatttt tcaattcatt ttcaagccgg atgatttgtt
     7381 ttttgtgtaa taataacttt ctcgtgcgca acggatcatg attaaaaata tttccttgtt
     7441 cataaggact gatatgaaca ttatgcaaga aagcttctcc gttgcggacg cgaataaatc
     7501 catcttttag attgatccgg ctgtttcgta tcgacttgat ctcggttcct tgcaagacca
     7561 tccctgcttc catcgtgtcg atgatcgaat aatcatggcg agctttcttg ttttgtgcaa
     7621 ttaattttcc ctcgcctttt ggcatagctg tttcctccaa ctcattttat tttttctttt
     7681 tcttcttttt aacacctttg taaaatggct tcttcccttt tttcttaccg aacgtttgct
     7741 tactatcgct atatcgtttg ttcttcttgc catttctttc tgggcgtttt cctgcttgtt
     7801 ttccttcgcg ttttcctgat tttccatttc gtgtttttgg ccgttccaac gctgtaacct
     7861 cttctgcgga aacaagttca aaatctactg ctcgttcatc tggatcagct ttgactactt
     7921 ttactcgcac tttctgtccg attttaaaag tcagaccagt tctttcacct acaagagcta
     7981 aatgattttc aacaaaatgg aagtagtcct gtttcaattc attgatatgg atcaatccct
     8041 ctactgtatt cggcagttcg ataaacaggc cgaatttcac aacagagctg atgatcccat
     8101 cgaattcttc ccccactttg tccatcatga attctgcttt cttcatggaa tctacttcac
     8161 gttctgtttc tactgcacga cgttccatct tagaactatg atctgcaatt tcaggaagtt
     8221 ccgtagccca cgaactttga ttgctttcac ttttatcttc actgtaagaa cggatcagac
     8281 gatggacgat tagatcggga tagcgtcgaa taggagatgt aaagtgtgtg tagtaatctg
     8341 ccgctaagcc ataatgccca aaattgtctt ctgaatagcg tgcttgttgc atgcttctta
     8401 gcaacatagt attgataact gcagcttctg gtttgtcttc tactccttcg atcacatgct
     8461 gtaaatcttt tggtgtgatc gttcctttcg ttcctcggac cagaatgccc aaagctgaag
     8521 caaaatcaaa gaagcgctgc attttttctt cttttggctg ttcatggatt cggtaaataa
     8581 acggcagatt taatttatta taatgttcag ctaccgtttc attggccgca agcatgaaag
     8641 attcgatcaa gcgttcacca attcctctgc ttcttagctg gatatcattt gggtgtcctt
     8701 cagcatcaac aaggattttt gcttcgcgat cctcaaaaga aatcgcgcca cgtttgattc
     8761 gcatcgtttc aaggattcga tgcaattcac ccatttcttg gaacataggt acgagctgag
     8821 catagcgttg catcgtttct tcgtcttgat cttctaagat ttgattgaca gctgtgtagg
     8881 tcatccgttc tgttgtttgg atcacacttg ggaaaatatc atgagagact acttgtccat
     8941 ttggatcaat ctccatttcg caactcatgg tcagccttgg aacatgggga ttcagtgaac
     9001 agataccatt cgataaacgt tgcgggatca tcggaatcac tcgatcagtc aaatatacac
     9061 ttgtccctct ttcatatgct tctctgtcca attgactgcc ttgtgtcaca tagtaagaaa
     9121 catctgcaat gtgtactcca agaaaataat taccgttatc taatttttta accgtaactg
     9181 catcatctag atcttttgca tcttctccat caatcgtaac aatgagctga tttcttaaat
     9241 ctctacgtcc agttatatcc ttttcgctga tttgatctgg aacttcatct gcttcagaca
     9301 atacatcatc aggaaatttc gtagggatac cattagccac gacaattgag aggatatcca
     9361 tgccagggtc attcttatga ccaatcactt gcttgattat cccttctaaa ctagtcgcat
     9421 accctttttc agggtaatgg gtcagttcga tcagcacaat acttccgtca actggacgaa
     9481 ctccctctgc tgctgcatat actttaaatt ggttgagctt tctatctttc gggataacaa
     9541 cgccgtaaag atccgtttct tgtatttctt cttctgaata agcaacgaat tcccctgcta
     9601 actgagtagt cgcccggtct cgaatctcga ctactttccc ctctgctccg cgatccatat
     9661 ctgtatttgc tcgcttcatg atatcgattg ctacggtgtc tccatccatt gcaaaaccag
     9721 tcgcttcttt tgggatatag atgtcatctt cttcgggatc aatcgttaca aatccaaatc
     9781 ctcgttcatt tgctcgaaat gttccttcga tcaagattgg ttttaaagga agtttcactt
     9841 ttcctttttt attgaaaact atactctgtt ctctttccat ttgagcaatc gtttgtacta
     9901 gtaatttaaa atcatcgctt ttttggaacc ctaaaccttc agcgatttct tccatagaaa
     9961 aactgcgttt gcgactgctt tccatgaaaa agagaatttt ctcttttatc gtttcttttg
    10021 tcatatttat tcctcattcc aaggtaattt ttctaaaaat tccaagacgt ctttttctaa
    10081 ttcgcgtctt gctgtaccaa ctgtaatcac atgaccactt tcaggatacc atcgaagtgt
    10141 cactcgctga cggctcaact tgcgtgcagt ttcaaatacg ccattagggt cgatcatttc
    10201 atctttccca gcttgagcta aaaatatcgg ctgttggata tctgacaacc tactttctac
    10261 gattgctgcc tgatcttgta tatccattaa ttgttgttca gctaacgagc ggtaagcaac
    10321 tgctttttca ttgatttctt tctctgattt acctgctgtc ttcagcactt gacgtacgta
    10381 tttttcaaag ttttcaggca cgtttgtttt tacaggagac aaaggcgaac agaaaaaacc
    10441 gccgccaatc actgattctt cttctagtgc tcgtacagca aagatgccgc ccattgataa
    10501 tcctaatacg gcaatatcag aaaactgctc agactgtaaa aaatggatgg cttttttagt
    10561 gtctgcccac cagctttctg ctttttcttg caaaatatca tatggctcca ttgttccgtg
    10621 tcctttaaaa agcggcgcat aaactgtata atctgctttt tccaaaaatc gtgcgagcat
    10681 tcgaacatcg tttggacttc ctgagtaagc gtgaagcagc aaaaccgctc gctttccatg
    10741 ttttgcataa aggggttttg gtaattgtct cactcgtctc acctctttcg cctcatttta
    10801 ccatagtttc tttcataact tcttcttaag cgttctcttt gcaataaaaa aagccgcccg
    10861 ccgttttgcg agaggcttat tcaactactt cgaagataaa tatgctagga caaataataa
    10921 gatcatccag atagctccca taactgcagt tgatcgctgc atgacagctt caaagccgcg
    10981 agctttttgt ttgccgaata gttgatccgc accgccggta aatgcactcg ctgcactatt
    11041 ttgcttgctt ggctgcatca taatcgcaat gaccataaca atcgataaaa cgatcacgat
    11101 tcccaaaatt aaattataca tatcgcagcc tccttcccaa aagatatcgt ctctttaact
    11161 ttatcataga taaaagtccg tgacaactct atataaatgg aataacagct gaaagttcaa
    11221 aactttcagc tgttattctt atgactgacg taagacgtct tgatattctg gtttcttttc
    11281 aaactctttt tttgcataag ggcataaagg aatgatcttt ttcccgtctc gtcttgctaa
    11341 ttctactcca gtatacacta gcttttcagc taacttttga ccacgatatg ctggatctac
    11401 aaatgtatgg tcaatgatca tgatgtctgg gcccgcatct gaccatgtca tttctccgat
    11461 ctcttgttgt tcatcattga ataaagcaag acgatttttt tcttctttga tttccatatg
    11521 ccgcactcct caaataactg atattttttt attcctacct tattttagca gtctcctgat
    11581 ctataaacaa ttatttatcc tcgaattact tacaaaaaag aagagaaaat taattcatcg
    11641 ttattctttg ttcaataaat gaaaaaatca ctgtccaata ggttttcgga tctttcatcg
    11701 atgcttccaa gtgttctgct tctggaacaa gcagcagtgc tttatcccct gccgtagcat
    11761 tttgaatcgt gtacgctgct tctgtaggga caaaatgatc tctttcacca tgaatgacca
    11821 ataaaggcag atggttgctg ccaagctgtc tcgtagctga tgcttgcttt aaggagtaac
    11881 cggcgtattt ctgggcataa tgattcgcat atctcatgac cattttttta ggaaagacag
    11941 tgattttact taacatatat cggaattctg cataaacaga aacatatccg ctgtctaaaa
    12001 taaatccttt gaccgctgat ggaagttttt caccacttgc catcatgatc gtagaagctc
    12061 ccattgatcc gccgtgtaaa ataatggacg cattcggctg ttcatctaag atgagttgga
    12121 tccaggcaat taaatctagc cgatctagcc atcccattcc gatgatttct ccttcacttt
    12181 ctccgtgcgc acgtaaatca ggtacaagaa cgttgaatcc tttctctgca tatcttttac
    12241 ctatatgaga catgtctctt ttccctgtgc ttcggtaatc atgtacgcaa atgacccatt
    12301 tattctgctt atttggatgg atgaacatct gaccagctag tttcaacccg tcataacttt
    12361 tgattgtttt gggtgttccc tgctcccaaa agctttcaca ctcttcttct aatttttgtc
    12421 gttttttttc tactgacgtg actgcagcag ggtccatcaa acgatgacca gtgtcttcat
    12481 accagtcata tcctctctgt actcccgctc ttactaattt ctttgcataa aaataagaaa
    12541 gaaaatggta actgatccct ataattatca gaataagaat gaaataaaaa ataaccattg
    12601 tacacccgcc gctttctgcc aaactataat ctaactatta tccaatgtac cgatccatag
    12661 gattctttat tttcggcaca cgtaaattcc atttcttttt cattgtatca tttacttaaa
    12721 gttctcctca atagatttac ttgtaagaga gcatttacat tcgcaataaa agccagtata
    12781 ctaatttcgt gcattataat caagacgctc gtcttggact ggttcggaaa cttcccagaa
    12841 taaaaaacta agtatctcta aagaaaggag attggaaata atgcgaaaag taattattga
    12901 ttgcgatcca gggatcgacg acactttagc tttatcatta gctgtcaaat cccctgatat
    12961 cgaagtagtt gctattaccg tagtttgtgg gaatgtccct gcagatatag gtacgcagaa
    13021 tgtgctgaaa tgcctggaac gctgtgatcg tttagacatt cctgtttatc aaggaatgga
    13081 aaaacctttg aagcaacctt tcatcagtgc acaagacact catggtatgg atggtttagg
    13141 ggaaacaaat ttcccgatga tcttgagaaa acaggcagaa cctctccatg ccgttgattt
    13201 tttggctgat tatttcaaag aaaagacaaa tacatcagtc attgctttgg gtcctttgac
    13261 caatatcgct tttgctttga aagtcaaccc aaatattggg aaacatatgg atcgttttgt
    13321 ctcgatgggt ggaacttaca aaagccatgg taactgttcg ccagttgctg aatacaatta
    13381 ctggtgtgat cctgaagcag cttcttatgt attcgagaat ctcaaacaaa cgatagaaat
    13441 ggttgggtta gatgtgacaa gagaaatcgt cttgacaccg actatcctcg aatactgctg
    13501 tcaaatgaat cctgaagagg gagagtatct aaaagcgatc actcgttttt attttgactt
    13561 ccactggaaa caagaaagaa ttttaggatg tgtcatcaat gatccgttag ctgttgctta
    13621 ttttatcgag ggggccatct gtgaaggttt caaaagcttc actgctgtgg aaactcaagg
    13681 aataagtcgt ggacaaacat tggttgatcg ttatgaattt tggcagaaac ctgctaacag
    13741 taaaattatg acaaaagtag atactcgtct ttttttccga aagtttttaa ccgttctttt
    13801 aaatgcacaa gaagaaacga ttatgaaaga tttggaaagg ttaaagatgg gctaacaaaa
    13861 tgaataaaaa caaaatggca actcgcgtac ttacagtgat ggctttgtgt attgggatca
    13921 attatattgg aggaacgatt gctttatggc ttcgtttgcc aatctatctc gattctatcg
    13981 gaacgatttt tgctggtgca ttgcttgggc ctgtacctgg aatgctgaca ggattgtcca
    14041 gcggtatttt aagtggagtg actacagata ttttttctct gtattattcg ccaatccaaa
    14101 ttctcacagg cttgcttgct ggattgattt tgccaagata tctcggcaaa gataaaaaca
    14161 gcttgaaact gttattactc gctttgattt tgtcggtacc tggaacgatc gtttcttcga
    14221 tcattaccgt tcagttattt ggcgggatca cctcctcagg atccagtatg atcgttcagc
    14281 tattacatgg gttaggttta agtcagacaa caagtgtgat cttggtacag gcaggtacag
    14341 actaccttga tcgtttgttg tctatcgtcg ttgtttcatt agtcatcact atgttgccac
    14401 aacgcttgat atattcaaaa agaaaaatag gctgaataaa cagagctgga ttagcaaaat
    14461 aatgctaatc tggctctgtt ttttcattta ttaaaaaaat cttacgctaa gtaggataaa
    14521 ataattcctt gatatttttc aatcatatcc aaataattat cgatttcaac atactcatct
    14581 acttgatgag gcaaagtaac gacaccaggc ccaaagacga caaagtcgaa ggaatggtta
    14641 gctttcgtaa attcggctgc atccgttgtt gctgccgctc caactaaagg taatggctgg
    14701 ctgaattgtt gctgaatgca atgaatcaaa ggagaatctg gatcagcttt gacaggtatt
    14761 ttgttgtaat cgatcatcaa ttccaaatga tagtctgtct cttgattcaa ttcattgacg
    14821 attgattgca gcaatgcaat gattttatca tttgggtatt ctggaatcga tcgaatgttc
    14881 ccttggagct gcgcatggct tgggatgctg ttgacttgat tcccgccact gatcaatgta
    14941 acattatgga ttgtttttcc taggacagga ttctcgattg tttcagcgag gtgattcatt
    15001 tcagcatttg ctttcgtaat gaattcattt aggtgattga ttgcattata accttggtct
    15061 ggcatagagc tatgcgcttc ttttccgtgc gaggtgactg tatagttgat tgatcccatg
    15121 tgcgtataca tcaaactgta attagttggc tctccgatga tcaaggcatc taagtcatct
    15181 acatagcctg ctttcgtgag ctgttcccca cctagttcac ccacttcttc tccgacagtg
    15241 gctaataatt tgactgttcc gttaaatggt tttcctgact ctttcagttc gatcatggca
    15301 atcaccattg ctgccaagcc actcttcata tctgtagcac cacgtccgta caaacggttc
    15361 ccatggattt ctgctgcaaa gggtgcatag gtccaagacg attcatcacc agccgccacg
    15421 acatccatat gcccagatag gcctaacact tttcctgatt ggcccttttg gtaacgagca
    15481 atcaaattat ctctaccatc tctgtaagaa acaatctctc ctgttatgtc atgctttgcc
    15541 aaaagcttat tcaaataggc tgctacttca ccttcgtttc cattcactga tttaattcgg
    15601 atgatctctt gtaaaatcgc gattttttct tcttttttca tctaatcctc tcccttgcca
    15661 tttcttttta ttgtatttga gtgtgctcga attttcaaat aggagcaacc aaagtaaaaa
    15721 tgaagaaaga gagccacttt atgaaaaata tgctgctact tccctcctat tttatttata
    15781 cgagaggact tgctcataga ttcctattct actgttctat ccttaaaagc tcttatatga
    15841 tgctagttat tgttagcttt cctttttcaa ggatgatctt ttcttgtacc attctttgta
    15901 aacatcatat acagaagcta gttgcaaaaa aaactctcta cctgctgata tt
//
LOCUS       DARBEG010000062        15856 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000062 DARBEG010000000
VERSION     DARBEG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.063"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            84..836
                     /locus_tag="SCK20_001965"
     CDS             84..836
                     /locus_tag="SCK20_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1146008.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            833..1747
                     /gene="pfkB"
                     /locus_tag="SCK20_001966"
     CDS             833..1747
                     /gene="pfkB"
                     /locus_tag="SCK20_001966"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1146009.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1765..3696
                     /locus_tag="SCK20_001967"
     CDS             1765..3696
                     /locus_tag="SCK20_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1146010.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(3807..5366)
                     /locus_tag="SCK20_001968"
     CDS             complement(3807..5366)
                     /locus_tag="SCK20_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG1146011.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5521..7755
                     /gene="pcrA"
                     /locus_tag="SCK20_001969"
     CDS             5521..7755
                     /gene="pcrA"
                     /locus_tag="SCK20_001969"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG1146012.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7772..9808
                     /gene="ligA"
                     /locus_tag="SCK20_001970"
     CDS             7772..9808
                     /gene="ligA"
                     /locus_tag="SCK20_001970"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG1146013.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9914..10219
                     /gene="gatC"
                     /locus_tag="SCK20_001971"
     CDS             9914..10219
                     /gene="gatC"
                     /locus_tag="SCK20_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG1146014.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10219..11691
                     /gene="gatA"
                     /locus_tag="SCK20_001972"
     CDS             10219..11691
                     /gene="gatA"
                     /locus_tag="SCK20_001972"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG1146015.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11688..13118
                     /gene="gatB"
                     /locus_tag="SCK20_001973"
     CDS             11688..13118
                     /gene="gatB"
                     /locus_tag="SCK20_001973"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG1146016.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13128..14183
                     /locus_tag="SCK20_001974"
     CDS             13128..14183
                     /locus_tag="SCK20_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1146017.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14294..15667
                     /gene="rlmD"
                     /locus_tag="SCK20_001975"
     CDS             14294..15667
                     /gene="rlmD"
                     /locus_tag="SCK20_001975"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1146018.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15670..15798
                     /locus_tag="SCK20_001976"
     CDS             15670..15798
                     /locus_tag="SCK20_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1146019.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT         5392 a         2903 c         3481 g         4080 t
ORIGIN      
        1 taatttttat gaacgttttt gactgattta tattgctagt agaaaaaaaa tatgctatta
       61 tttgtattga ggtgatcgtg aaaatgctta cagaggaacg cagacaaaaa atcctagact
      121 tgcttgaaca gcagaaaatc gtgaaatcac aagatcttgt aaaccttttg aatgcttctg
      181 aatcaacgat aagacgtgat ttacaagagc tggagaatag tgggtttcta gaaagagttc
      241 atggcggagc taagaaagag caattattga attttgagca gaatatgagt gaaaaatcac
      301 tcaaaaacgt tcacgaaaaa cagcaaatcg ccaaatcggc agctgaaacc attaatgacg
      361 aagatgtcat ttatttagac gctggctcta ctacgttaga aatgattcct tttctgcaag
      421 gaaaacagat cacagtagtg acaaattccg ttcaacatgc ggcaaattta gttgatttgg
      481 agcttgctac cattatttta ggtggaacga taaagctggc aactaatgct gttttaggat
      541 ctaatgcgat agaacaacta aaaaattttc attttaataa agcatttatg ggaatgaacg
      601 gtgcccatct tgaacaagga ttcacgactc cggatccaga agaggcagct gttaaacgtt
      661 tagccatgaa aaattccgaa gagacatttg tattgattga tcataccaaa ttcaaccagt
      721 tgtccttcac ctctgttgca cctttggaag cagccacgat cataaccgag cgttgtccat
      781 tagaattcgt taatgaattc cgtgaaaaga caacaattaa ggaggcaata caatgattta
      841 cacagtaaca cttaatccat caattgatta tatcgtacgc gtagatgggt taaaactagg
      901 cgatttgaac cgaatgaagg aagacttcaa gttacctgga ggaaaaggga tcaatgtttc
      961 ccgtattttg aaacggatcg attctcaatc tacagcacta gggtttttag gagggttcac
     1021 tggcgaattt atttcgaagt ggcttgaaaa agaacaaatc cagtctttgt tcacaaccgt
     1081 aaaagaagat acaagaatca atatcaaact aaaatcagac actgaaactg agatcaatgg
     1141 cttaggccca tctatttcag atgaagaaat cgatgagttg aagcaagcat taggaacagt
     1201 aggacaagga gatatcgtcg tattgtcagg aagcgcgcca gctagcctgc gaaaaggatt
     1261 ttatcaagaa ctgattgata tcattcgaga aaaaggtgca gatttcgtga ttgatacaac
     1321 aggtgatgac ctaaaaaatg cacttgttaa gaaaccgttg ttgatcaaac caaacaatca
     1381 cgaattagct gagctttaca acacaacctt ccaatctgta gaagaaatca ttccgtatgg
     1441 gaaaaaatta ctagctgaag gggcacagaa tgtgctgatc tcaatggctg gagacggtgc
     1501 tttgctcttc acggaacaag gaacttataa aacgaatgta ttagttcgcc cattgaaaaa
     1561 ttcagtaggt gcaggggatt caatgattgc tggctttatt ggggcttaca gccgtacgaa
     1621 agatccaatc gaagccttta aatggggagt tgcttgcgga agcgcaacag ctttttctga
     1681 tgacttggca gctcgcgaat tgattgatga gttgctgcca gaagttgaaa tcacaaaaat
     1741 tgattagttt caaggaggat aatcatgaat atcaaggatt taatggtcaa agaagcgatg
     1801 atcatggatt tgcaagcgac tgataaaaaa ggcgcaatcg atgaaatggt ccaaaaaatg
     1861 tttgatgccg gccggatttc cgatatcgaa acatataaag aaggaattct agcccgcgaa
     1921 gcacaaactt ctacaggtct tggtgatgga atcgccatgc ctcacgcgaa aaacagtgca
     1981 gttaaagaag caacagtttt attcgcaaaa agccaaaaag gagtagatta tgaagcctta
     2041 gacggacagc caacttatct gtttttcatg atcgcagctc cagaaggcgc aaacgacaca
     2101 catttgcaag cattagcagc actatctcga ttgctgattg atgcagattt cgtaggaaaa
     2161 ttaaaagaag caaatacacc agaagaagtc caagacttgt tccaaacagc tgaagagcaa
     2221 aaagaagcag aagaaaaagc agaacaagaa acacaagctg cagaagcttc tgctcatcca
     2281 gaaagaaaat tcgttgttgc cgttacagct tgtccaacag ggatcgctca cacttacatg
     2341 gcagaagatg ctttgaagaa aaaagcgaaa gaaatgggtg tggagatcaa agtcgaaacg
     2401 aacggttcag aaggaatcaa aaaccgattg actgctgaag acatcgcccg tgcagaaggt
     2461 gtaatcgttg cagcagacaa aaaagtagag atgaatcgct ttgacggaaa agaactgatc
     2521 aatcgtcctg tcagtgatgg tatccgtaaa ccagaagagt tgatcaatct agcccttagt
     2581 ggtactgcac cagtattcca tggtgatgga aaagaaagca gttcagaaga aggaaatgcg
     2641 gatggtacta tcggacaaag aatttacaaa gatttgatga acggcgtatc acacatgtta
     2701 cctttcgtta ttggtggagg aatcgctatt gcgctttctt tcatgatcga ccaatttatt
     2761 ggcgtgccac atgatcaatt atcaaatcta gggaattaca atcaagctgc tagctggttc
     2821 aaccaaatcg gacaagctgc atttggtttc atgctgccgg tattagcagg atttattgct
     2881 tcaagtatcg gtgaccgccc tggtttgatc gttggatttg ctgctggtgc attagctaat
     2941 acaggcggtg caggattctt aggtgcactg attggtggat tcttagctgg ttatgtcatc
     3001 gtatttttac gtaaattatt caagaactta ccaaaatcat tggatggaat taaaacgatc
     3061 ctcttttatc cagtgtttgg cttactgatt actggtttct tgatgttgct ggtcaatgtc
     3121 cctatgaaag caatcaatga tgcattgaac ggtttcttga caagtatgag tggttcgaat
     3181 gctgctttac ttggtgcatt attagctgga atgatggcgg cagatttagg cggtccaatc
     3241 aacaaagccg cttatgtgtt tggtacagca actttagcta cgactgttgc aactggcgga
     3301 agtgtcgtga tggcttctgt tatggctgga ggaatggtac ctccattggc aattttcgtt
     3361 gctacacgtt tgtttaagaa taaattcagc aaaacagatc aagatgctgg tttgacgaac
     3421 atcgtgatgg gactatcgtt tgtcacggaa ggatcgattc catttgcagc agctgacccg
     3481 attcgtgcca ttccaagttt tattattggt tctgctttaa ctggcggatt agtcggagct
     3541 ttcggaatca aattactggc tcctcacggc gggattttcg ttgtattctt actgagtcac
     3601 ccggtcatgt atttggtgtt tattgctatc ggagcagtcg tatctggtgt gatttatggt
     3661 ttattgagaa aagctcctgc agtagcagaa gcatagaaat aaaaaagaag aggctgggtt
     3721 ttcccagcct cttctttttt tatagaatca tatagaatca agtagtgtag atgaatagtt
     3781 gttgtaaaat agtttttcca tgttcgttaa aatgattgct tcaaatttgt gatagaagat
     3841 ttagcctcga tttcctgctt gattcttggc aagtttgctt catcagtcaa gataaccaaa
     3901 atatctccta cattcatgat cgtatcaccg tgagtcaaga tttcctgatt tcctctgcgt
     3961 atagagatta gcagcatatt tttaggccaa ttgaagtctc gtaccattaa tccgtctaaa
     4021 gaactctctg ccgttactgg aaaatcaaaa gttgtccttg caccttttag tgaggcggtc
     4081 tgagtatttc caagtagacg ttccaataat gattcgtaaa ttggattacc gccgagaagg
     4141 tcattgacga tataagcaac aagagcagcc aatcctaaag gcatcagatg attaagagag
     4201 ccaaccattt ctgtcactaa aataattgct gtcagcggtg cttttccaat cgctgtgaaa
     4261 taaccagcca tggcaataac gacaaaatcc ttgatgtaga ttggatccat cccgaaatca
     4321 accataatgt ttccataaaa gctaccgatt aaagccccca atgtcaatat cggaaggaaa
     4381 atcccccctg gaagaccccc accgtaagaa atcatggaaa aaacgaatcg aagaacaaac
     4441 aaaccgataa tcataatgat tggcaaggaa gcatgtccaa aagctaaaac gatttgattg
     4501 cctccgccta gagtactagg ccataaataa ccaattggca aaatcagcaa gaaaggaacg
     4561 aacccgtgaa aataagctgg taaaggcaat cggttgtaaa attttggcaa agccagcaag
     4621 acattctgat aaataaaacc taagatgcct agaagtactc ctaagatcac taaggaacca
     4681 taatatttca acggcaaaga aggaaggtca cctagatata aaacaggtct caagccgaaa
     4741 aagtaaagag agacgaagtt agcagtgatt gctgaagcaa atgatgtcag ccaaacaagt
     4801 ggagaaaagg agtgatggat ttcttctaaa acaaagagta atcctgcgat aggagcattg
     4861 aatgctgctg caagtccgct acttgcaccg ctagaaatta aaatcttttc ttctgaagat
     4921 ggactccgaa acaattgtcc agctccttgt ccaatggatg cgccaagctg tatagaaggt
     4981 ccttctctac ctagaaaaag gcccgcacca actgaaagta caccgccaat gaactttttc
     5041 cataaaacag agaaccagtt cattgataat tccccgcgaa gctgtccctc tacttgtgga
     5101 atcccgcttc ccttgatatc cggttcattt ttgaccagat aacccacgaa aaatgctgca
     5161 aggatgcagc aaatagctag gccaattaag taaatcggct gttcccgact catttcatag
     5221 atacttcgaa ctaaatcgct tagcttttcg atagataatc gaaacaggct aacgacgatt
     5281 cctgtcagca gtccaacaaa tattcctttt agaataaaat atatctgagt cttatctaat
     5341 ttcttgattt ttcctttttt ttccattcaa acgcctcgct ttcttttaat ttagcgcgaa
     5401 atcaacgaat tgacaaggaa aaatccataa aaagaaacaa atgattttga actagctagt
     5461 ttaatgaaag ccaagaaaat gctataatta acaagattga taaaagaaag gatgttgtta
     5521 atgtcatcca aagctgaatt attaaacggg atgaaccccc gtcagaaaga agctgttctt
     5581 cacactgacg gaccattact gctaatggca ggtgcaggaa gtggaaagac gagagtgctg
     5641 acacaccgca ttgcctattt gattgaggaa aaagaagtca atccttggaa catcctagcg
     5701 attactttta ccaataaggc tgcaaaagaa atgaaagaac gtgtcaatgc gattctcgca
     5761 tctggaggcg aagatgtctg ggtatcgact tttcactcga tgtgtgtgcg tattttaaga
     5821 agagatgttg attttatcgg gtataaccgg aactttacaa tcatcgattc ttctgaacag
     5881 ctgaccttga tgaaacggat actaaaagaa ctgaacattg atccgaaaaa atatgatcct
     5941 cgaagtatat taggaacaat ctctcaagca aaaaacagcc tgcagacccc tcaggatttt
     6001 gcaaaaatgc aaggcagtta ttacgaagag attgcggcaa aatgttatgc tgcttaccaa
     6061 aaagagcttc aatataatca atgtatggac tttgacgacc tgatcatgaa tacgattcga
     6121 ttatttgaag aacatcctga ttcactcacg tattatcaaa acaaattcca ttatattcat
     6181 gtagatgagt accaagatac aaatcacgca cagtatacgc tagtcaattt attggcaggt
     6241 agatttagga acctgtgcgt cgtgggagat gcagatcaaa gtatttacgg ctggcgcggt
     6301 gcagatatgc agaacatctt ggattttgaa aaagattatc cagatgctgc agtaattttg
     6361 ctagaacaaa actatcgctc cacgaaaaac atccttagcg cagcaaacca agtcattgaa
     6421 aacaatagca atcgaaaacc aaaaaacttg tggacagaaa acaaagaagg aaacaagatc
     6481 acttactacc gtgcagataa tgaacgagac gaaacccgtt ttatcgtaga ccggatgcaa
     6541 gaagagattc gaagcaatca tcggaattac ggcgactttg ccatcttgta ccggacgaac
     6601 gcacaatctc gtgtaatgga agaaactctg ttgaaagcaa atattcctta taaaatggtt
     6661 ggagggcaca agttctacga tcgtaaagaa atcaaagata ttttagcgta tttgaatgtg
     6721 ctggtaaatc ctcaagattc aatcagtttt gagcgtatcg ttaattcgcc caaacgaggc
     6781 atcggaccag gatctatcga aaaactacgc agttttgctt ctcttcatga atggccactg
     6841 cttgaagcag cgcaaaatgt ggatctggca aatatcggtg gaaaagcagg acagcaatta
     6901 ggtgcatttg gagagatgat ccaagaagtc actcagatga tcccatactt gacagtgaca
     6961 gaattgacga aagaggtttt ggatcgtagc ggatatctag aagacttaaa aatccaaaat
     7021 acattagaag cacaagcacg catcgaaaac ttggaggaat ttttaacggt cacacaagaa
     7081 ttcgacaaac aattcgaaca gcaaaatgaa gaagacgctg atgcaccaga agaaaaacta
     7141 acggtcttct taaatgatct ggcacttgtt tccgacattg acaatttaga agaagatgct
     7201 tcccaagtga cattgatgac tttgcatgca gccaaaggat tagaatttcc agttgtattc
     7261 ctgattggtc tagaagaggg ggtttttcct ttatctcgag cattgatgga agaaagcgaa
     7321 ctggaggaag aacgccgtct cgcatatgtg gggatcacgc gagcagaaga agcgttgtat
     7381 ttaacaaatg ctttctccag aacattatat ggacggacgc aatataaccg accttctcgt
     7441 tttgtagaag aaatcgatca ggagttactg gaaatcgaag gcatgcgtcc tacgcctaaa
     7501 aaaacgcctg tattcgctaa aaaaacagca tatagctata aacagccaga gacagcggtt
     7561 gttccctcta aaagtgctac tggaggggaa aataacagct ggaaacctgg agataaagtc
     7621 aaacacaaaa aatggggtct aggaacagtc gtccgagtaa gcgggacgtc gaaggatcta
     7681 gaactagatg tggctttccc aagtcaagga gtaaaacgtc ttttggctgc ctttgcgcca
     7741 attgaaaaag cttagtagtg gagggtaaat gatggaacat gaaatgacga ttgacgaagc
     7801 tgctaaacga gcagaagaac ttcgtacaag actgaaccaa tggtctcgcg aatactacgt
     7861 agaagataaa ccaacagtgg aagactatgt ttatgataaa gaatacgcag aattagtagc
     7921 aatcgaggaa caatatcctg atttgatcac gtctgattct cccactcaac gagtaggcgg
     7981 aaaagtatta gaaggttttg aaaaagtcac tcatgatatt ccgctttaca gtttgaacga
     8041 cgttttcagc aaagaagagc tgatagcttt tgatcaacgt gtacaaaaag cagtgggacg
     8101 tgtagttgac tattgctgtg aactaaaaat agatggactt tccgtttctt tgcgttatga
     8161 agatggaaat ttcgtgagag gtgcgactcg cggggatgga actgtaggag aaaacatcac
     8221 tgaaaaccta aaaactgtcc ggtcggtccc aatcaaatta aaagaaccaa tgaacatcga
     8281 agtaagaggc gaatgtttca tgccaaaacg gtcatttgtc caattgaacc aagacagaga
     8341 agcagaggga aaagatatct ttgcgaatcc tcggaacgcc gcagctggtt ctttgcgtca
     8401 gttagattct aaaatcacag cgaaaagaaa tctggacact tttttataca cagtagctga
     8461 tttcggtcct atgcaagcaa aaacccagta tgatgcgcta gaagagctag aaaaaattgg
     8521 attccacacg aatcgggaaa aacgtctatg tcattcgatt gatgaagtgt ggtcatacat
     8581 tgaagaatat catgacaaac gtgtggattt gccctatgaa atcgatggaa tcgtcatcaa
     8641 agtgaatgaa ttctcgctac aagatcaact tggttttact atcaaagcac cgagatgggc
     8701 agcagcttac aagtttccgc cagaagaagt tgaaacgcta atcgaaaaca tcgaatggac
     8761 agtcggtcga acaggggtag taacgccaac agctatcatg acaccagtcc gagtagcagg
     8821 aacgacagta agccgtgcca gcctgcataa cggtgattat attaagttga aggatatccg
     8881 cttgaaagat acagttctga tttataaagc gggagacatc atcccagaag tttcgcaagt
     8941 cgtgttagat aaacgaccta aagacagcga agaataccaa ttgcctacac attgtccagt
     9001 ttgtggaagt gaacttgtcc atttggatga agaagtagcc ttacggtgca tcaatcctaa
     9061 atgccctgct caaatgaaag aaggtttgaa ccactttgtt tcaagaaatg cgatgaatat
     9121 cgatgggttg gggccgcgcg tactagaaca gatgtatgat aaaaaactag tagcagatgt
     9181 tgctgatctt tacaaattga cagaagaaga gttattgacg ttggacaaaa taaaagaaaa
     9241 atcagcaaat aatattttga cagcaatcga caacagcaaa gataattcgg tcgagcggtt
     9301 gattttcggg ttaggaatcc gacatgtcgg agcgaaagca gcaaaaattt tagcagaaca
     9361 tttcggtgat ctagaaacat tgagtaaaag tgactatgag tcgattattg cgcttgatac
     9421 gatcggtgat atcattgccg acagtgtagt cacttatttc agtaacgaag aagtccatga
     9481 attgatgaat gaattaaaac aagcaggcgt caatttcgaa tacaaagggc ttcgaaatgc
     9541 ccaattgcaa gaagtcgagt ctccttttaa agaaaaaaca gtagtcctta ctggtaaact
     9601 aacacgattt actcgtgaag aagcaaaaga gacgatcgaa aatcttggcg ggaaagtgac
     9661 tggtagcgta tcgaaaaaaa cggatatcgt ggtagcagga gaagatgctg gcagcaagct
     9721 gaccaaagca caagaattag gaatcgaagt ttggacagaa gatcaaatgg cagatgcatt
     9781 ggctaaaagt cgctcagtag aagaatgaaa aatgagtttt ttcagacaaa tttcaataaa
     9841 caatttcaga cacggcactc ttgtgttaaa atagagagca aagtttttaa ctcgtttaga
     9901 aagaaggggt agtatggcga ttagtgaaga acaagtgaaa cacgttgcca aattagcaaa
     9961 acttgcgttc gcagatgaag aacttgcaag ttttaccgac caattaggta aaatcatcga
    10021 tatggttgaa atgctggaag aagtagatac ggaaggtgtt ccatttacgt cgaatgtggt
    10081 tgaaacagta aacgtgatgc gacaagatcg ggcgcaagct ggatggaacc gcgatgaatt
    10141 attgaaaaat gtgccggaaa cagaagacgg ctttatcaaa gtacccgcaa tcatcgataa
    10201 tggggaggct ggcgcataat gacagaatta cacaatcgtt ctctagaaga actgcatcat
    10261 atgcttgtat cgaaagaaat cactgtacaa gatttgacaa aaacaacatt tgaacgaatc
    10321 aaagaaacag agcctgaggt cgaggcattc attacattga acgaagaaaa agcattggaa
    10381 caggcaaaag ctttagatga aaaagggatt gatgaaagca acgtccttgc tggtattcca
    10441 attggtatca aggataatat cgtaacaaaa gatatcttga caaccgctgc atctaagatc
    10501 ctttacaatt ttgaaccaat ttatgatgca acggtcatgg ataaaatcta tcaagcagat
    10561 atgatccctg ttggtaagct aaacatggat gaatttgcta tgggtggaag tacagaaact
    10621 tcttatttca agaaaacaaa aaatgcttgg gacccaacaa aagtacctgg tggctcttct
    10681 ggtggttctg cagcagcagt tgcagctgga caaattccag tttctttagg tagtgatact
    10741 gggggaagta tccgccaacc agcagctttt aacggcatcg tagggatgaa acctacttat
    10801 ggccgtgtct cacgttttgg tttgattgcc tttgcttcat ctcttgacca aatcggacca
    10861 atgacccgta cggtgaaaga taatgcatta gcattgacag ctatcagcgg ttatgacgaa
    10921 aaagacggta cttcttcagg cgtttcagtt cctgattttg cagagggact gacaggcgac
    10981 atcaaaggaa tgaagattgc tttacctaaa gaatacttag gcgaaggtgt ggcccctggc
    11041 gtaaaagaag ctgtattgaa agcagcagaa acattccgtt cattaggtgc tgttgtggaa
    11101 gaagtcagct tgcctcattc aaaatacggt gtggctgttt attatatcat tgcctcatca
    11161 gaagcaagtt ccaatttgca acgattcgat ggtatccgct acggttatcg ctctgaaaat
    11221 gtgaaatcat tagatgatgt atatgtcaac tcacgttcag aaggattcgg tgaagaagta
    11281 aaacgccgta tcatgttagg tacattctcc ttaagcgcgg gttattatga cgcttacttc
    11341 aaaaaagcag gacaagtccg tacattgatc aaacgagatt ttgaaaaagt ctttgcagat
    11401 tatgacttga tcatcgggcc atcttcacca acagttgcat tcggaatcgg tgagaacatt
    11461 aacgatccaa tcacaatgta catgagtgac atcttaacga tccctgtcaa cttggctgga
    11521 cttccaggta tgtctgtacc ggcaggttta tcagaaggat taccagttgg attgcagatc
    11581 atcggtaatt attttgatga aaaaacgatg tatcaagcgg catatgcgtt tgaacaagca
    11641 acagaattcc ataaacaaca accagctatt ttaggaggga aagacgaatg aattttgaaa
    11701 cagtcattgg actagaagtc cacgtcgagt taaaaaccaa ctcaaaaatt ttctctccag
    11761 cgccagctca ttttggtgca gaaccaaaca gcaatacaaa cgtgatcgac tggggttatc
    11821 caggcgtgct gccagtaatg aataaagctg cgctggaatt cgggatgaaa gcagctttgg
    11881 cattaaactg tgaaatctct aaagatactc attttgaccg gaaaaattac ttttacccag
    11941 ataatccaaa agcatatcaa atttcccaat ttgatcaacc aatcggtcat gacggatgga
    12001 tcgaaatcga agtagaaggt aaaaagaaaa aaatccggat cgaacgtgtc catttagaag
    12061 aagatgctgg aaaaaacatg catggtattg gcggttattc ttacgttgat ttgaaccgac
    12121 aaggcacgcc tttgatcgaa atcgtttctg aagctgatat gcgttcacca gaagaagcat
    12181 acgcttatct agaagcattg cgatcaatta tccaatttac agaagtcagc gatgtgaaaa
    12241 tggaagaagg ctctatgcgc tgtgatgcca atatttctct tcgcccttat ggacaagaag
    12301 agttcggtac gaaagcagaa ttgaaaaact tgaactcttt atctttcgtg aaaaaaggtt
    12361 tagcttttga agaaaaacgt caagcaaaag ttcttctttc aggaggagaa atccaacaag
    12421 aaacacgccg ttttgacgaa acaacgaata aaacagtttt gatgcgtgtc aaagaagggt
    12481 ctagtgatta tcgttacttc ccagagcctg atatcccgaa attcgtgatc gatgacgaat
    12541 ggattcaaaa agtaaaagct agtttgcctg agatgccagc ttctcgtcgc gaacgctata
    12601 tccgtgagtt tggacttcca gagtatgacg caatggtatt gacacttaca aaagaaatgt
    12661 ctgatttttt tgaagctgtt ttggaaaatg gtgcagatgc caagcaagca tctaactggt
    12721 tgatgggtga agtttctgct tacttgaaca gtgagaaact ggaattagct gagacaaaat
    12781 tgacacctga gaacttagca ggaatgattc gtttgatcgc cgatggaacg atcagctcaa
    12841 aaatcgccaa aaaagtcttc cgtgaattga tcacaaatgg cggagaagcc caagaagtag
    12901 ttgaaaaaaa tggttgggtg caattatctg atccgaacaa actactgcca atcattaatg
    12961 aaattttgga taacaaccaa caatctgtcg atgatttcaa aaatggaaaa gatcgtgcgg
    13021 ttggcttcct agtcggtcaa atcatgaaag caactaaagg acaagcaaat cctggtgtcg
    13081 tgaataagct acttaaagat gaattagcga aacgatagag gctgaaaatg aaaaaagcac
    13141 gcgtaattta taaccccacc tcgggaaaag aattattgaa aaaaaatttg gcggatatcc
    13201 tacaggcatt agaagaagca ggatttgaag ccagcgccta tgcaactaca ccagaaccag
    13261 attcagccaa gaatgaagca tgtagagtcg cagaactagg ttttgatcta gtagtggcag
    13321 ctggtggcga tggcacaatc aacgaagtgg ttaatggaat cgctccgctt gaacaccgtc
    13381 ctaaaatggc catcatccca gctggtacaa cgaacgacta tgctcgagca ttaaaaattc
    13441 ctagagataa tattaaagca gcagcagaag tgataaaaaa acaccaaacc gtaaaaatgg
    13501 acatcggcct ttctgataac agttacttca tcaatatcgc tgcaggtggc tatttgacag
    13561 aattgactta tgaagttcca tcagaactaa agagtatttt tggttatctt gcttatcttg
    13621 cgaaaggagc agagatgctc cctcgagtaa agccaatcaa aatgcgcttg aagtatgatg
    13681 aaggggaata tatcggaaat gcttcgatgt ttttcctagg gctgacgaac tctgttggag
    13741 gattcgaaca gatcgcgcca gatgccaaat tggatgatgg gaagttttct ttaatcattg
    13801 tgaagacagc aaatatcttt gagatcttgc atattgcagc actcatgctg aatggcggtc
    13861 gacatgtcga tgatccaaga ataatctata ccaaaacaag cagtttgttc gttgaaacag
    13921 aagatgatat caatcgacca gtaatgatca acttagatgg tgaatacggc ggggaagcac
    13981 cgatgcattt ccaaaatctt catcaacaca ttgagttctt tgccaatacc gatcagattc
    14041 ctgatgaagc aatcacaggt acagatgaag aagaattaga ggttgtcagc aaagaattcg
    14101 taaaagaagt cgaacgttta acggatgaag atatcgacgg tgacggaaaa atagcagacg
    14161 aaacacctaa tcaaaagaat taatcaaaag ccggtgctga acgtagtaag gtcagtgccg
    14221 gttttagttg tgtaattagc tgatttatag tagagttcag taggattgaa aaataggctt
    14281 aaaggagcaa aatatgacag aacagcaaat agtaaagaaa aacgaacgat atacagtcga
    14341 aatcgtcgac ttgagttatg aaggaatggg ggtagcaaaa atcgatggtt atccattatt
    14401 tatcgaaaat gcactccctg gagaagaagt agaaatacta gtagtaaaag caggcaataa
    14461 gtttggatat ggaaaagtcg aaacatttat tacacaatca cctgatcgtc agcctgtaga
    14521 aaacaatgca ttacttcgca taggtatcgc acctttagcc catttgaagt atgaacaaca
    14581 gcttgtcttc aaacaaaaac aagtagagca tgtaatggcg aagattgcta agatgccaga
    14641 agttcctgta cttccaacgg tcggaatggc agaaccattc ggctaccgta ataaagcaca
    14701 gatccctgtt cgccgtatcg atggacagtt agccaccggt ttttacaaaa agaatagcca
    14761 tgatctagtc cctatcgaag atttttatat ccaagatcct gtcatcgatc aagcaatcaa
    14821 agtagtcaga gatatcttgc aacggttcca agtacgagga tataatgaag aaaaaaatga
    14881 aggacagctt cgtcacatca ttatccgtcg tggtcattat tcccatgaaa tgatggtcgt
    14941 tttagttact cgtaaagaaa aattctttaa atccaaggaa attgctgaag ctattcatga
    15001 agaattgcca gaagtggttt cggtcatcca aaatatcaat gaagaaaaaa caaatgtgat
    15061 tttaggagac aaagaaaaag ttttatatgg cagagactat atcgaagatc agctattagg
    15121 aaaaacctat cgaatctcag caaagtcatt ttatcaagta aatactcaac aagcagagta
    15181 tctgtaccaa acagccatcg agtttgccga gttagataaa aaagacattg tgatcgacgc
    15241 ttattcagga ataggtacga ttggcttgtc tttagcagac agagtggata aggtgtatgg
    15301 gatggaagtt attccagaag caattgaaga tgcacagttc aatgctttaa ataataaaat
    15361 cgaaaatgtt cattatgaag tcggaaaagc agaaaaagtt atgaaaaaat ggcaagaaaa
    15421 agggatcaaa ccatctgtga tcgtagttga ccctccacga aaaggattag atcatcattt
    15481 tattgaatca gcaatcgaaa tgtcaccaga acgaatcgtc tatatctcat gtaatccggc
    15541 aacttttgca agagatgcta agttatttgc agaagcaggt tatgccgtga agaaagttca
    15601 gcctgtagat cttttcccgc aaacccatca tatcgaatta gtcgcttcat tcgttaaagg
    15661 agaataaaaa tggaccaaaa agaaaaaatg aaagaattga tcaaaaagaa acaaggtgga
    15721 ggccgatcca aacaaatgga aacacctaag aatgatcgta aaaacatgag aaaaggccct
    15781 aaaatcttta ataaatagac gtgctaaaga aagactaaga agtgttaact tttgttattg
    15841 tttcttagtc ttttta
//
LOCUS       DARBEG010000063        15725 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000063 DARBEG010000000
VERSION     DARBEG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.064"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(285..651)
                     /gene="ssrA"
                     /locus_tag="SCK20_001977"
     tmRNA           complement(285..651)
                     /gene="ssrA"
                     /locus_tag="SCK20_001977"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(822..1595)
                     /locus_tag="SCK20_001978"
     CDS             complement(822..1595)
                     /locus_tag="SCK20_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1146020.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1739..2089
                     /locus_tag="SCK20_001979"
     CDS             1739..2089
                     /locus_tag="SCK20_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG1146021.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2070..2786
                     /locus_tag="SCK20_001980"
     CDS             2070..2786
                     /locus_tag="SCK20_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1146022.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2786..4234
                     /locus_tag="SCK20_001981"
     CDS             2786..4234
                     /locus_tag="SCK20_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1146023.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4304..4813)
                     /locus_tag="SCK20_001982"
     CDS             complement(4304..4813)
                     /locus_tag="SCK20_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146024.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4803..5528)
                     /locus_tag="SCK20_001983"
     CDS             complement(4803..5528)
                     /locus_tag="SCK20_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146025.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5751..7871)
                     /locus_tag="SCK20_001984"
     CDS             complement(5751..7871)
                     /locus_tag="SCK20_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1146026.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8101..9279
                     /locus_tag="SCK20_001985"
     CDS             8101..9279
                     /locus_tag="SCK20_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG1146027.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9295..10053
                     /gene="aroD"
                     /locus_tag="SCK20_001986"
     CDS             9295..10053
                     /gene="aroD"
                     /locus_tag="SCK20_001986"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG1146028.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10076..10561
                     /gene="ybaK"
                     /locus_tag="SCK20_001987"
     CDS             10076..10561
                     /gene="ybaK"
                     /locus_tag="SCK20_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG1146029.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10632..11885)
                     /locus_tag="SCK20_001988"
     CDS             complement(10632..11885)
                     /locus_tag="SCK20_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1146030.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12003..13352
                     /gene="gdhA"
                     /locus_tag="SCK20_001989"
     CDS             12003..13352
                     /gene="gdhA"
                     /locus_tag="SCK20_001989"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG1146031.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13464..14810
                     /locus_tag="SCK20_001990"
     CDS             13464..14810
                     /locus_tag="SCK20_001990"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG1146032.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15118..15504)
                     /locus_tag="SCK20_001991"
     CDS             complement(15118..15504)
                     /locus_tag="SCK20_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1146033.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15553..>15725)
                     /locus_tag="SCK20_001992"
     CDS             complement(15553..>15725)
                     /locus_tag="SCK20_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1146034.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         5137 a         2814 c         3163 g         4611 t
ORIGIN      
        1 aaaacttatt tgaaggaaca tgcttaaacc ataattgtgt ttttccttcc atcagcataa
       61 aagttcacct caaaaagagt gtacgaacaa aagttctttt tgtaaacctt aaaatagatt
      121 ttcctgaata cgtattgggg atttctaaat gtttgaatgt ttttttgaat gcaaaatcaa
      181 tgcaaaaaat tttgtatttc tctacttttc tctccttgac caaaataaaa aaacgttgat
      241 ttaacaacgt ttttcataca tgtttatctg tctcaaccat acatatatgg agacggcggg
      301 agtcgaaccc gcgtccaaac acatcgccac ttaaaaatct acgttcatag tcaactcatt
      361 taaggattca ctttacagct tgccgagtga caggcattct gtatcgctag tctgatgttc
      421 tctttttcaa cttacagacg gaaagttgaa acgtatccca ctaaagttag gacccttact
      481 cgagcacatg ggcgatgccg agaggatcta cgctaactgt ttttaggcag ctaaagcgta
      541 agagttgttt tcgtttttag cagttatatt taactgtaac gtttttacgt agacgtaacc
      601 tacgaaacgc aattcaagct cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa
      661 caagggttac gtgtattttt catacaaaat tagtatagca tatattggga taaattcaat
      721 aaaaaagcga atcatatccc attatttata taataactgc ccctttctta agaaaaaata
      781 agcgattttc ttaagaaaag aggtagcttc aaaaaaagat cttaaatcag atcaaaatac
      841 ttcaatgcgt ggacaattcc gccttctgta tttttctttg tgataaaggt agaaagttct
      901 tttaatccgt cataggcgtt gcccatagcg attttgttgt cgcaagcttc caacaaggca
      961 aaatcattcg gaccatcgcc aaatccaaaa gtaggaactt tttccagctg aagttctttt
     1021 tgcagacgtt tgacacctgc cccttttgag attcctttct tcactgtatc gatcgagtaa
     1081 ggaccattcc gataaaaagt tagctcagga aaatgttcgt gatagtattc gtcacctgat
     1141 tgagataaga tcagcaacat atttactgtg ttttctttaa agcctaaagg atcaatttcc
     1201 ggaacatctg aatgaatgaa gtcgtaagca ttgatcattg tttggctatg accggtacac
     1261 cagattttct gatcgttata gtaagaaagt tcatgaccgt tcaactgtac atgatcatac
     1321 attttttgac attcttctga agaaaatgtt tgagaataga tttcttcgcc gtctactcga
     1381 atgaaagaac cattcatggt gaccgcagaa gtaatgcccg catcttccat gatagagtgg
     1441 atttctgctt ctgtacgtcc tgtagcgatg acggggagaa cattgttttc ttttaatgcg
     1501 ttcatagctt ttgccacttc gggtgtgatt tttgatttcg catctagtaa tgttccatca
     1561 agatcaaaga aagtgattgc acgataattt gacacaaata aaactccttt tttattggaa
     1621 taagtttatc ttaacaaaga atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca
     1681 tattggttca gtcaacaatt tagagtatac tataagaaga gaatttagga gggaaagaat
     1741 gatttttttc agtattggta tcttattctt aataattggc agtctttttc ggattctgcc
     1801 ctcaaaagga aatctgcctt tttatgggta tcattcgcct cttgccgcaa aaacagacgc
     1861 tcattggcgg ctggctcaaa agacaagcgg aaactggttt tttcttatgg gcttactgat
     1921 ggcattgatc ggctattatt taaagacatc tgggcatacg aattattttc tgattgaaat
     1981 gcttctgcta gtatttccaa ttatgcctat ttttattatg acagaaaaga aattacagaa
     2041 atatgacctg gaaactggag ggaacgataa tgaatatttt aatgattgaa gataatcaat
     2101 ctgtttctga aatgatgcag atgtttttct taaatgaagg ttgggaagca actttcaagt
     2161 atgatggaaa agaagggctc gatgcctttt tagaaaaccc acaacattgg gatatgatta
     2221 cactagacct gaatttgccc acactggacg gtatgctagt aggaagagag atccgcaaag
     2281 tttcaagtac tgtgccgatt atcatgctga cagctagaga ttctgaaagc gatcaggtga
     2341 tcggcttaga gatgggggca gatgattatg tcacaaaacc tttcagtcct ctgacgctta
     2401 ttgctcgaat gaaagctttg catcgccgta gtgagttggc agaagcaaga gaagaagcag
     2461 cagtttcaga tgatcatttt gatatcgaga ctagtcattt caagatgaat acgaaaacac
     2521 gagaagcgta tctggacaac cggccaattg aaggactgac gcctaaagag ttcgatctat
     2581 tgtttacttt agcaaaaaaa ccacgtcaag tattctctcg ggagcagttg cttgaattag
     2641 tctgggatta tcagtacttt ggtgatgaac ggacagtgga cgcccatatc aagaaattac
     2701 gacaaaaaat cgaaaaggtc ggaccacaag tcatccaaac tgtttgggga gtcggctaca
     2761 aattcgatga ttctggtgtt gcctaatgcg ttatttgtat cagcaactct tagctttttg
     2821 cggaatggtc gcgttgatct tattgatcgt tggagtttct tttacacaac tgacaaaaca
     2881 aacgattgaa gaaaacaatt accagcaact gtttggatat gctgaatcag ttgaaaagac
     2941 aacgcaaacc tatgccgata cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc
     3001 attgctgctg acagagcaag tactgacaga acaaaatgtg aactttatat ttatcgataa
     3061 aaacgaacgt gtcatttatc cgactaatac agctttactg aattttacct taacagctga
     3121 tcaatggaaa aatctgagga atggacgtca agaaaaattc acatcgaata aaaatatttt
     3181 aggacaagca caggcgacct cttacgcact tgtgccattt aatttgaatc atgaattcta
     3241 tggagcactt gtagttagtc aacctgcgcg aaatatcgat aacagcgttc gtccaattac
     3301 actgaacctg ttcaaaggat ttattttttc cagtatcgtt gcagttattg ccagctatgc
     3361 ttttgcagca ttccaagtta agcgtatcaa tcggttaagg aatgcgacga aagaagtgac
     3421 gaatggtaat tttgatgtac agctccccgt acatgacaaa gatgaatttg atgagttagc
     3481 agatgatttc aataaaatga cgaattcatt gaaagaatct caagcagaaa ttgaagaaca
     3541 agaaaatcgt cgaagacaat ttatggcaga tgcttctcac gagatgagaa cacctcttac
     3601 gacaatcaac ggacttctgg aagggttaga atacaatgca atccctgaaa atcagcgaga
     3661 aaacgcgatt aaattgatga aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa
     3721 cttggattat gaaaaaatcc gtacgaatca aatcagtatg gtcattaaaa aattcaatgg
     3781 aacagaaaca ttgaaaaata ttgttgcaca gttagaagct aaagcagaag cagcaggaga
     3841 tacattgaca ttgaaagccg ttgatgatat tgatgtttac gctgattatg accgatttgt
     3901 ccaaatcatg gtcaatatta ttcaaaacgc gatacagttt actgaaaacg gccaaatcac
     3961 gattacttta gaaaaaggct atcttgaaac gatcatcacg attgaagata cgggaattgg
     4021 aatgtctgaa caacaaatga aaagtatctg ggatcgttat tataaagtag atccttcaag
     4081 aaaaaataca aaatatggtg aatcaggatt aggtttacca atcgtgcagc aacttgtacg
     4141 cttgcataaa ggaaaattag aagtagaaag cgaactagga aaaggaacaa aattcgctgt
     4201 tcgtttgcct gacgtagaaa tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga
     4261 atcgatcttc tataccgctt gtttttacta taaaatgatt gatttagccc gcgggctcta
     4321 aacgatcaat ataaggactt tgagcaatag tagcatcatg agtaaagagt aagatacctt
     4381 gattttcttc atgagctaaa taagaaagca agtggagtag ctcttggatc tccaatacac
     4441 taagttcatc aaatgtatct tcaataacaa tattgtttcg cttggaaagc aaggcatgaa
     4501 gtaattgcag ctttactttt tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg
     4561 aaggagagat atcaagtttt tcttgatagg ccaaacgatc ttgtttgatt tctttttccg
     4621 gtacatccaa caggacatgg tctttaagat tcagataagg aaacatcggc caattttttt
     4681 ggatgatcgc aaaattatcc agttttgcaa caagaacctc ttttttcaag tgctccaata
     4741 ttttatgctg tttttcgaga ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat
     4801 attcaggttt cattacgtcc aggcctccta ctttgcaaat gatggataaa aaaagtgaaa
     4861 gggaatagac taagaatgag acagcatcca ctgagcccaa taagagtttg ggcaaatccc
     4921 ttaagtatat ctgtgaaaaa aagatcattt tggaaatcga tggtaaaaaa agtctgttga
     4981 tccatcggaa ttgtaagtcc gcttaccgaa gtcaagatat cacggatatc tggcagttca
     5041 gtagtcttta agacaagaaa attgatcctt tgaagaagcg cttcataaac tgactggaag
     5101 cttacgatcc acaataaaac caagatacaa caaagaaaga gaggaaagaa cagctgccag
     5161 aaaataaaaa ggaataattt ggttttagaa agaccagtca ggcgccaatt gatcaattcc
     5221 tgcttgtttt gcctgactga atagtaaaag aaaaagataa aaaggaaggc aaatagaaaa
     5281 aataaactga atatgccttt tatataaaat cggctagcta attctaaagc cggaagaagc
     5341 tctgtagatg cagaagcgcc aataaatgaa agccgatctt ggacttgatt ggctaaagcc
     5401 gattgcaaat ggtaaagagt caggcatgct atcaataaaa aagcagaaaa gcttgaaaat
     5461 aaacaataga gtattgtctg ctttttgtaa taaattaatc gagcagtagc atagcggatt
     5521 attttcatac ttttcacctc tataaaaagt atagaaaatg aatatgaata aagtatgaaa
     5581 ttcctttagt gaaatcacga attaaaacca gaaaatattt tgtaaaaagc aagaggttgt
     5641 gaagaagctg ccctattcta ttaccgtaga tgcaagcttt tgaacactgt ttattcgaaa
     5701 agaggagcag cgacaataat tttgtcgctg ctccttttaa atttattttg tcattcggaa
     5761 gaacttgtgg tcgttccgtg aagttcttgg tacgtttgcg ttttataaag atcaacagtt
     5821 gattgtccgt tgtgttcact aaagacacta gttgacttgt caccaagaga tttttcagct
     5881 tcttttaatt tctctaagcc attcttgtag tcataatcac tggtatcaat tgggtctaat
     5941 ccactattcg tatagaaccg aagaagatca ccattattga tttggtcaga aacagctaac
     6001 tgtttatcaa ctttttcttt cagtgaatca atttcattct gtacttcctc agtaggttca
     6061 gtaatagctt caccagtttc gttactatag atcgtaccac cgtagtaagt atattttggt
     6121 gtaacaaaat caccatcacg gaaagaaaca agctgttcat gatctttcga gaatagatct
     6181 tgtcctaact gaatataatt ttttgtatct acacctagta agtgaagtaa tgtaggcaag
     6241 gcatcaactt gtccgccgta agtgtgattg attccgccct gtgtttgtcc agggatatgg
     6301 atcatataag ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt
     6361 tttccaacta attcagctag atttttattt cttgaattcg agacaccgta atggtcaccg
     6421 taaaggacga tgactgaatt atcatacaag ccgctttctt tcaaataatt gaagaattct
     6481 tgtacggcgg tatctaaata attggctgtc gcaaagtaac cgttgatcgt ttcatcattt
     6541 gtattggcta acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg ataatggtta
     6601 gatacagcaa tgaattttga ataaaatggt tgttgcagac gttccaagta ttgtacggat
     6661 tgttggaaaa atggtttatc atgcaacccg tattggaacg aattgtcatc attaacatca
     6721 taataagaag aatcaaagaa ataatcatat cctaaattct tgtatgtctc gtttcgattc
     6781 cagaatgtac cagcgttccc atggaatacc gcactagtgt aaccttgtgt ttgacttaaa
     6841 atatcaggtg cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa ggagccttga
     6901 ttcaagccaa acaatgaatt ttccatcagc gtttccgcat cgcttgtttt tcctgctttt
     6961 acttgatgga aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag actattgatg
     7021 aaaggtgtaa cttcatgctg gagaccattc tcatctgtca atttataatc gatcaggaat
     7081 tgctggaagc tttctaaatg gatataaatc acattttttc ctttagcgag cccgaacaaa
     7141 tcgtcatttg gtgctgcata atgttctttt acataatttt ctacttcttt catatcattg
     7201 ggacttgctt ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg
     7261 aaagcattca agccaagata tttgaccaaa taatcacgag agaatgttcg agtcagcaat
     7321 tctggacgat cagcttccgc cataaaaaga tttcctgaga aaatcataac tgctaatgta
     7381 gagatcgcaa aagccattct tgctcggatc gggcgagggt ccaacttgat tttttttgca
     7441 atcagcagaa cagtcaagat aataaaatcg atccaataga tcacatcata tgggcgaaac
     7501 agtcgaatgg cactttctcc cagaccactc gctactttcc cagcacctaa aatcgtgttg
     7561 atcgtaatga agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc
     7621 aaaagaaaat aaatgaccat catcgtgata taagcaactc tagggcgtct gatataaaga
     7681 gcaattccaa gtaataacaa tgtagttgca atagggttga taaacaagat aaagtattga
     7741 atgccacttt cgattccgag atggaaatca actaaatagg caaaaatcgt tttcaaccaa
     7801 agtaaaacga ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt
     7861 atacatttca agataatctt cctttctgca cttcttaatc acagaagtgt catgaaacca
     7921 tgttacacag ctcacttatt ctcatttatt ttactagtat gacaatatag tgtcaattga
     7981 aaccagaagt ttatgctgtt tcattaaggt tttctttaag cttcatgaaa aaatgagcag
     8041 taagacaagt cctatgtttt ttgttatact tgggaagaga aagctaaagc aggtgaagga
     8101 atgaaagtaa aagtaaaaaa aagagcaagc aatcgattga aaaaaggata tccactgatc
     8161 caaaaagaag accttgctca tccccaaaaa gaaaccacgc aatgggtgga attagttgat
     8221 tcgcaaaacc aatttatcgc aaaaggatat ctaggaaagc agaataaggg aatcggttgg
     8281 gtattgagcc agcagaacat cccatttgat caatcttttt ttgaagaatt ttttgtgaag
     8341 tcaaaagaaa aaagaacttt ctttttttca gatgattcta ctaccgcttt tcgtatattc
     8401 aatggagaag gagacggaat cggcggtctg attgttgatt attatgcagg ttatgctgtt
     8461 ttttcttggt ataacgaaac gttatatgca catcgtgaat tattgattca agcttttcgt
     8521 gaaatctacc cggaaatcgt aggaggctat gagaaaattc gatttgaatc agtaaatctg
     8581 cctgagagcc agttcattta cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac
     8641 ggagtaaaat ttgccgttta tttaaatgaa ggattaatga ctgggatctt tttagaccaa
     8701 aaagaagtac ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg
     8761 tttagttata ccggcgcctt ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca
     8821 agtgtcgatc tagcaaaacg cagtttaaaa aaaacacagg aacagtttga agtgaatggt
     8881 ttggatgtcg atcagcaaaa aatcattgtt atggacgtat tcgagtattt caaatacgcc
     8941 aaaagaaaac aatttttata tgacatgatt gtattggatc caccaagttt tgccagaaat
     9001 aagaaaaaag tctttcgcgt agcaaaagat tatggggaac tggtcaagga tagtctggat
     9061 attttagcag aagacgggat acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa
     9121 caattccaaa caatgattga agaggaattc catgccaaaa atgtttcttt tgaacaactt
     9181 gctttatatc gtctgccaaa agatttccag acgacggata cctttttaga aggcaattat
     9241 ctgaaagtgt ttgtttatca aattaaaaat aaccattaga aggagcgatg cagaatggtt
     9301 tgggtaaagg gattgaatat aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg
     9361 gccgtccgag aggaagtatt acgtgaagca gttcttgcac gtgaagcagg ggccgattta
     9421 gtcgaatggc gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca
     9481 ttggttctta tccatgagat gcttgatgga ctgccgttat tatttacgtt tcgtacctta
     9541 gctgaaggcg gagaacaaga catatcgatc cgtcagtatc gcgaccttta tgaacatgtg
     9601 atacacacag gattagtaga tatggtcgat atcgagttgt tcaaagtaga aagtctagga
     9661 aaaagactga tagaggaaat caagtcttta aacattcctt taattatcag tagtcatgat
     9721 tttaaagaaa caccagcaga ccctgtcttg ctttaccgat tgaatgttat ggaacatttt
     9781 ggagccgata tcggaaaact ggcagtaact cccaataatg aacgagatgt tttgcggttg
     9841 atggagttga ctagacgggc aaatgctttt gtctccatgc ctattattac gatgtccatg
     9901 ggaaatcttg gtaaaatttc tcgtctggct ggcggaacga cgggttccgt tatgacattt
     9961 ggttccttaa gcagcgattc tacttctgct ccaggacaat taccggtcaa agcattgaag
    10021 caaattattc aattattaaa tgaaccgttg tagaacaagt ttgaggtgat attttttggc
    10081 aaagaaaaaa atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta
    10141 tacagagcac gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca
    10201 gcttccagaa agccaatctc gtatatttaa aacactggtt gctgtgggca atgtgacggg
    10261 gcctcttgtt gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt
    10321 ttcagggaat aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa cgaccggata
    10381 catacgtggt ggctgctcgc ctataggtat gaaaaaacta tttcctactt atttagatca
    10441 aattgcagaa agctatgaac aaatcattgt ctcggctggc agaagagggc tacagatgga
    10501 gttagcacct caagatatct gtgcattaac gagcggacaa tttgcagata taaaacaata
    10561 aatcatacgt ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc tccttctttc
    10621 ttttttatac attatgctct ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa
    10681 gccgctgtga tattaaagcc gccagtataa ccattgacat ccagtaattc tcccgcaaaa
    10741 tacagaccat caatgatttt actctccaat gttttaggat aaatttcctt tagagaaacc
    10801 cctccgccag ttacaaatga tttctcaata ggaaaagttt tgttgattga tagcttaaac
    10861 tctttgcata gttgaacaaa atcttgaatt tgtttttgag aggcttgttg tcctgtcaga
    10921 tgatccattt caagccgctc aagaaagaaa actaacaatc gctcaggcaa tataccatgc
    10981 caagcattga ccaagttctt tttggttgct ttactttttt ctgtcaatat atgaataagc
    11041 tcttgtttgg tttgtgtagg gaaacaatcc agagaaagtg tcactggttc tcccgttttc
    11101 tctaattctt tattgacaaa acttgaacaa cgcagtgcag ccggaccaga aataccgaaa
    11161 tgggtgaaaa gtaaatccat cgtatgttca gtcaatacac gacctttttt atttaaaacg
    11221 cgtaaagtga tgtcttgtaa agaaagccct tgaagtgttt tctcctgaat atacggttca
    11281 tccgatatca agggggactc tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc
    11341 acgatcttgt acccatctcc agtagaacca gtagatggat aagtccgtcc accagttgtt
    11401 aagataatgg caggagcatg gaactcttcg aattccgtgc gtataccgta aattttatgt
    11461 tctttgtgga tcaatttctc tactcgagta gagaatagaa gcgtcacatc tagttctttc
    11521 agacgattga gcaatgcttc gataatcgtt tttgatttat ttgttacagg aaacatccgt
    11581 ccatgatcct cttcttttaa atgtacgcct tgtgattcaa agaagttcat gatatcgaaa
    11641 ttattccatt gcgcaaacgt actatataaa aactttccat ttccaggaat atgtgcaatc
    11701 aaatcatcaa caggacgatt gttcgtaaca ttgcaccgac caccacctgt catcagtagt
    11761 ttttttcctg cttttttgtt tttttcgatc aagagtacgc gtgcgccttg ttcagctgca
    11821 ctgatcgctg ccatcatccc gcttgttcca gcgccgacta cgatcacatc atacagtttt
    11881 tccattccct cacctcaagg aaagtgtagc atatctgaag aaagaaaaca gaaaaaaaat
    11941 gttttgagcc aaaagagaaa atgagctaaa atgagggaag ataagcaaag gagcgtggaa
    12001 cgatgacaaa cgcaacagaa tatgttcaag caattcaaga aaagctacat caaaaggatc
    12061 aagggcaaga agaattttta caagcaatcg atgaattcat gcccacggtt atgtcttttt
    12121 tggacactca tcccgaatat atcgaaaaga atatcttaga acttttaacg gaaccagaaa
    12181 gagttattca atttcgagtg ccttggcaag atgatgaagg aaactggcga gtaaatagag
    12241 ggtaccgtat ccaatacaac tcagctatcg gcccttataa aggtggattg cgcttccatc
    12301 ctagtgtcaa tctaagtatc ttgaaatttt tggcttttga acagattttc aagaatagtt
    12361 taaccggtct gccaatcggt ggaggaaaag gcggaagtga cttcgatcct aaaggaaaat
    12421 ctgataatga gatcatgcgt ttttgtcaaa gtttcatgct ggaattgtca aaacatatcg
    12481 gcccatcgat tgatgttcct gcgggagata tcggtgtagg agctagagaa atcggctatt
    12541 tgttcggcga atataaacga ttgaaaaaat atgatactgg tgtgttgaca ggtaaacctt
    12601 tggatttttg gggaagtaag atacgtacgg aagctactgg ttatggatta gtgtattatg
    12661 tcaaacatct attgaacgaa gaaaaagatt catttaatca aaaaacagtt tttgtttccg
    12721 gaagcgggaa tgtagccatt tatgcaatcg aaaaagtgca agagctaggc gggaaagttg
    12781 ttacatgttc agattctagc gggtacatct atgatccaga aggaatagat gtgaaactat
    12841 tgaaagaagt caaagaaatc aatagacaac gtttgacgga atacgcaaac caacgtcctt
    12901 ctgctattta tcatgctgga gaatctgttt ggacattgaa agaaaaagcg gatatcgctt
    12961 tgccatgtgc tactcaaaat gaaatagacg aaagcttggc cgaaatctta gtagaaaatg
    13021 ggatcaaaat cgtagcagaa ggcgctaata tgccatgtac agtaaaagct gtttctgttc
    13081 ttcatgaagc aggcgtgtgg tattgtccag gaaaagctgc taatgctgga ggagtagcag
    13141 tatctgcttt agaaatgagt cagaacgtcc agcgattgac ttgggaaaga gagcaagtgg
    13201 atgagcagct ggatcaaatc atggaaacga tttaccatac gtgtcgccaa acagcagccg
    13261 agtacggcga cgaaaaaaat ctgcttcttg gagcaaatgt tgctggattt gaaaaagttg
    13321 ccaaagcaat ggcagcagaa ggtttagttt gaaaaatgaa actttcttgg aaatattgtc
    13381 agcattttgt ttatttgctt gaaaaataat aaaaaaaaac gtaaagtatt acctgtagag
    13441 taaacctata aggaggagaa caaatggcac acattcattt tgactattca aaagtagcac
    13501 cgtttgtcaa cgaacaagag ttagggtaca tgcaaagcca agtaacagca gcccacaacg
    13561 aattacggga aggtactggc gcaggaagcg acttccgcgg ctggatcgat ttaccaacga
    13621 actatgacaa agaagaattt gcacgtatca aagaagcagc taaaaaaatc caatctgatt
    13681 ctgaagtatt agttgttatc ggtatcggtg gttcttattt aggcgctcgt gcagcgatcg
    13741 acttcttgaa tcacacattc ttcaatttat tagatagcaa cacaagaaaa gcaccacaaa
    13801 tcttctttgc cggaaattca atcagctcaa catatattgc tgacttgatc gaagtaatcg
    13861 gtgatcgcga cttttcagta aacgttattt caaaatctgg aacaactact gaaccagcga
    13921 ttgcgttccg tgtattcaaa gagcttcttg ttaagaaata tggacaagaa gaagcaaaca
    13981 aacgtatcta cgctacaaca gataaagcaa aaggtgcagt taaagtagaa gcagatgcag
    14041 aaggttggga aactttcgtg attcctgatg acatcggcgg acgtttctct gtattaactg
    14101 ctgttggttt gttaccaatc gctgttagtg gtgcagatat cgatgcattg atgcaaggtg
    14161 ccgctgatgc aagcaaagca tactcaagcg acaaactaga agaaaacgaa gcttaccaat
    14221 acgcagcaat gcgcaacatt ctttaccgta aaggcaaagt aactgagttg ttgatcaact
    14281 atgaaccagg aatgcaatac ttctcagaat ggtggaaaca attgtatggt gaatctgaag
    14341 gaaaagacca aaaaggcatc tatccttcaa gcgcaaactt ctcaactgat ttgcactctt
    14401 taggacaatt catccaagaa ggacgtcgca atatttttga aacagttgtg aaggttgaaa
    14461 aacctcgtaa atcaatcaca attcctgaac aagcagaaga tttagacggt ctaggctact
    14521 tgcaaggaaa agaagtggac ttcgtaaata caaaagcatt tgaaggaaca ttgcttgctc
    14581 atacagacgg tgacgtgcct aacttgctag tcaaaattcc agaaatggat gcttatacat
    14641 tgggttatac aatgtacttc tttgaaattg ctgtaggtat ttcaggttac ttgaatggaa
    14701 tcaacccatt cgaccaacca ggtgtagaag cttacaagaa aaacatgttc gcacttctag
    14761 gcaaaccagg ctttgaagat cttgctaaag aattgaacga acgcctgtaa gataagggtt
    14821 tttcgctaga tttaaataat cagtgataga aacatgttcg ctttaaaatg aaaactggta
    14881 cccatttagg tactcaaaca tactgcatcg tttattcaga ataaggggct gtgacaagac
    14941 gtttgtcaca gtcccttgtt tgtatagaaa tcgattgatt gccttgctct ttagtttgtt
    15001 ttaggacttt aagaacagtg aggctgactt cgaaggttaa ttctgttttt ttgttttcta
    15061 gaatcagtaa gcaaaaaagc actcacttcg agtgcttttt tgcgatatat tcttagctta
    15121 agataggcct aatttttctt ttacagatga gggtacctta tcgctggtaa ttactttttt
    15181 ctctatgatc cgcttttcgc ttgctttcaa ctgcaagatc gttccttcgg ggtaagcaga
    15241 accattggca gagtcaccag aagtaatgac atcgaaatct aactgtcttt tttcaccgtt
    15301 ttcagcataa gcatctacgt tgtatttaaa acctcttcca attacttccc cattatctgc
    15361 tttgatatca gtttctgcag gcattggagc tttgactacc gcataataat ctttacctac
    15421 ataagtagac tgatagtagt cccaaccttt ccatcctata aaagcaatca atatgataac
    15481 gatcaaccct aaaatttttt tcatgaaaga tctccttttt tatttttatt atacccgtgg
    15541 tgctagttaa ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc
    15601 tcgacttaat agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt
    15661 aacagaacgt gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtctc
    15721 aactg
//
LOCUS       DARBEG010000064        14938 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000064 DARBEG010000000
VERSION     DARBEG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.065"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            28..537
                     /locus_tag="SCK20_001993"
     CDS             28..537
                     /locus_tag="SCK20_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1146035.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            630..1792
                     /locus_tag="SCK20_001994"
     CDS             join(630..906,906..1792)
                     /locus_tag="SCK20_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1146036.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2072..3052
                     /locus_tag="SCK20_001995"
     CDS             2072..3052
                     /locus_tag="SCK20_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1146037.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3174..4592
                     /locus_tag="SCK20_001996"
     CDS             3174..4592
                     /locus_tag="SCK20_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG1146038.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4663..6132
                     /locus_tag="SCK20_001997"
     CDS             4663..6132
                     /locus_tag="SCK20_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1146039.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6142..6609
                     /locus_tag="SCK20_001998"
     CDS             6142..6609
                     /locus_tag="SCK20_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1146040.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6645..7517
                     /locus_tag="SCK20_001999"
     CDS             6645..7517
                     /locus_tag="SCK20_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1146041.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7598..10132
                     /locus_tag="SCK20_002000"
     CDS             7598..10132
                     /locus_tag="SCK20_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1146042.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10288..11061
                     /locus_tag="SCK20_002001"
     CDS             10288..11061
                     /locus_tag="SCK20_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1146043.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11054..11845
                     /locus_tag="SCK20_002002"
     CDS             11054..11845
                     /locus_tag="SCK20_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1146044.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11855..12346
                     /locus_tag="SCK20_002003"
     CDS             11855..12346
                     /locus_tag="SCK20_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1146045.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12364..12774
                     /locus_tag="SCK20_002004"
     CDS             12364..12774
                     /locus_tag="SCK20_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1146046.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12778..14178
                     /locus_tag="SCK20_002005"
     CDS             12778..14178
                     /locus_tag="SCK20_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG1146047.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14201..14575
                     /locus_tag="SCK20_002006"
     CDS             14201..14575
                     /locus_tag="SCK20_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1146048.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5190 a         2098 c         3008 g         4642 t
ORIGIN      
        1 tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg tgtttatgtg
       61 gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa taatttaatg
      121 acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag tacgagtatg
      181 attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga attagctgat
      241 cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga gaaaatgaat
      301 cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc gttaacaaaa
      361 tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta tagctcaata
      421 aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca gattctagaa
      481 aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga agattgacat
      541 gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa ggtgtgtaaa
      601 ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga acctattcaa
      661 aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga gctgaaatca
      721 ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa gcgcaaacta
      781 ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta attgcattac
      841 gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg gcgctgatat
      901 tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat cagcgatgtg
      961 taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag ttgaagatga
     1021 atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag cttatggtac
     1081 caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac gcagaatcgg
     1141 ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt tcaaattcca
     1201 ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct tcctacagga
     1261 aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga agaaatggca
     1321 gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt catgcgggga
     1381 gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact cgttgaataa
     1441 tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga ttgacgaaat
     1501 tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct atgataacgc
     1561 cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat accgctttga
     1621 gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga ataccatccg
     1681 gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga gattggcgca
     1741 gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt aattatttga
     1801 atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg cagtcggaac
     1861 accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt tgccattcca
     1921 catatatctt taaacagcta ttgccagata aaaataaatg atattatata tataaatact
     1981 tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt cgagtaggta
     2041 gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta gagaagttgc
     2101 gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc gtggatattt
     2161 gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat actatcctaa
     2221 tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga tttttcctac
     2281 aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc tttccagcag
     2341 gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa aagagtactt
     2401 gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca ataaagatat
     2461 tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc ttggtgaaaa
     2521 tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc aactacttgt
     2581 aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa agctacccgc
     2641 gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg aaccgttagt
     2701 cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta agacatatct
     2761 ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg cttctttggc
     2821 gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag tgattggatt
     2881 tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc aacagcctat
     2941 agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg gcgaaaagga
     3001 gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat aagttaaatc
     3061 ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga catatgaaac
     3121 gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag aaaatggaga
     3181 aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat gtttgggtcg
     3241 gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa cgtgatacta
     3301 gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg aaagattttg
     3361 ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag agagaccagt
     3421 ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt tatacagggt
     3481 ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat gctgagagtg
     3541 atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca caagaagggt
     3601 atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa gaagaatatt
     3661 tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga cgtttagtgg
     3721 aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg gctccgggat
     3781 tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg tatcttgtct
     3841 attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt ttggaaggtc
     3901 cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca ggtagaacag
     3961 cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat caagaaaatg
     4021 ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat caaaaagagg
     4081 atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata aatttacatg
     4141 atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg cttgtcaaaa
     4201 atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat cttagtgact
     4261 tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat cgcttcaatt
     4321 tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt caatatatgg
     4381 ataaaggact tattagacct atatctttaa agcctaacca atcttataaa ctaaaagtga
     4441 ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac gctcgtgtac
     4501 atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg actgcaataa
     4561 ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg caggtctgaa
     4621 aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta caaaaaaatt
     4681 gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc tacacactgc
     4741 attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga aatgcttgag
     4801 gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt aattttcgga
     4861 acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc taaggaagta
     4921 tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact tcaacgagct
     4981 tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg ggcagcaatg
     5041 gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga tggctcattc
     5101 gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat tgcaaccaca
     5161 tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg cggaaaccct
     5221 attttaggat tacttgtagg atttgttatg attcatccag gactagccaa tcggaatgat
     5281 ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag cattccagca
     5341 gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt agcaaaagtt
     5401 gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt cccaactgtg
     5461 acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg gaattttatc
     5521 ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc ctttggtgct
     5581 tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca tgctattcaa
     5641 ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc tatttggtca
     5701 gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt taagaaaaaa
     5761 tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg tattactgaa
     5821 ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg ctcgtttatt
     5881 ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat tggacaagga
     5941 ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat atttggtaat
     6001 ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata taaagtgttt
     6061 atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa attgccagtt
     6121 caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat aagcgttgta
     6181 gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt cttttcaaaa
     6241 ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt atctccaatc
     6301 gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa aacaaaatca
     6361 ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg tgagggattt
     6421 gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat gaccattaat
     6481 tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt tccagactac
     6541 caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga attgtttgtt
     6601 cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt tttggggcta
     6661 ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg aacatatttg
     6721 aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt ttagattttt
     6781 ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt cctattgata
     6841 ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt gcttggcagg
     6901 attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct tggacgactg
     6961 atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc ctttcaagtg
     7021 ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac ggaaacttca
     7081 ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac aataaagaca
     7141 acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct gctggtccgg
     7201 caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac gttacttggg
     7261 aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg ttatcacctg
     7321 atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag aaagtacaag
     7381 ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac aattatatta
     7441 tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta gcaagagacg
     7501 ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg gttgatgcga
     7561 tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa tgaaagatga
     7621 agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc aagatattgc
     7681 aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt tattttcaga
     7741 tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata atcaacaaaa
     7801 gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat ccactataga
     7861 taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa taaaaggaaa
     7921 cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc ttgatagaaa
     7981 agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg caaataatcc
     8041 agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg gggctgaaaa
     8101 agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag atgaggttca
     8161 caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc aaaagtatcg
     8221 taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct tagccacaac
     8281 agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag ctgaaatcac
     8341 tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat ttgaatttct
     8401 taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac tttttactga
     8461 tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa tcaagcttct
     8521 ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag gggacaaact
     8581 agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa tgtttcttaa
     8641 atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa ttgaagaaag
     8701 catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg agtataatta
     8761 tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa tatcaccaat
     8821 tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca gtctatttat
     8881 aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta agttcagaaa
     8941 atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga gtagtttaga
     9001 agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg taaagaagtt
     9061 ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa taacagcttc
     9121 tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg ctttcgatat
     9181 gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt gtaaaagtat
     9241 agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac aaatgtatga
     9301 aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt ctacagcttt
     9361 agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt tttaccagat
     9421 ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac aaaagaaaaa
     9481 tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg atattttaaa
     9541 acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat taaagaaaat
     9601 agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt caaccactga
     9661 attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg ggaatcaaac
     9721 cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct taaatgaatt
     9781 agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag attccaaaaa
     9841 agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg atgtcaaaat
     9901 cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg aacgaattat
     9961 gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat ttgatacttt
    10021 tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta ttgaaatacc
    10081 caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct aaatagcata
    10141 ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat ttaattaata
    10201 tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt gcttatataa
    10261 aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt agttgtgttg
    10321 tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc ccaacctgtc
    10381 gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact ttatatcggt
    10441 gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc agtaccggat
    10501 tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca ggatgcttct
    10561 ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga tgttctaaag
    10621 tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa agaagattat
    10681 caaaaattag agtggatgca atatttggca tcaattaata caagctttgt atctggaatt
    10741 cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat caattatatt
    10801 cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc tgttgggata
    10861 ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt agttggtttc
    10921 gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg cgctgcaatc
    10981 gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc tgcaacagga
    11041 ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa ttttccggtg
    11101 gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg gttatgcgta
    11161 ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa tgaaacaatc
    11221 cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt acattattgg
    11281 tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag taagtgctct
    11341 gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg tagttattcc
    11401 atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt ttgttggaat
    11461 tattttgtgg atactggcat caattttatt aaggattact gcttatcctt tataccttgc
    11521 tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa agtctattac
    11581 tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca cagttgtaaa
    11641 agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa atgaaatctt
    11701 tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt attatttgtt
    11761 aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt ctattgcgtt
    11821 aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga aaggtattat
    11881 acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat ggacaaatca
    11941 attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg acgtaaaaaa
    12001 agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta caagggaagt
    12061 agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta ttgttgcagt
    12121 ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa gtgtaaacgt
    12181 aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa atattactga
    12241 agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga cagtgataca
    12301 aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac agaggatgag
    12361 tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt agatacatta
    12421 aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga tgcaggcatt
    12481 gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca agaagttatg
    12541 gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag aagtgaagta
    12601 tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat tgaggctgtc
    12661 aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag aaatatgaat
    12721 acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga gtgataaatg
    12781 caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc tttttataaa
    12841 gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc tttaactgat
    12901 ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga atacggggaa
    12961 gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg aagtattgtc
    13021 caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc taagttggat
    13081 ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca ttggaaaaag
    13141 gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga ttggcgcgat
    13201 ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg agctagaaaa
    13261 ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga agatttaaaa
    13321 gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat tgaaatgcct
    13381 gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg tgaagataaa
    13441 aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc agtagacgaa
    13501 gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga aactggccgt
    13561 tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa ctgggattgg
    13621 ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct tggtgttaaa
    13681 ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag ttttaagtta
    13741 gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga ttattttcta
    13801 tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa attatttgta
    13861 gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa tcagcttact
    13921 ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct gcaacgacca
    13981 ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga tacgatagct
    14041 gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt cggaacagca
    14101 ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta ttcaataaat
    14161 tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa atggtttaca
    14221 acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg aatccttagg
    14281 gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt tcgagttgaa
    14341 tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag ccattgatca
    14401 tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag atttaggata
    14461 tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa tccgttactt
    14521 taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga aataaataga
    14581 taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt atcagcattt
    14641 caatagttta atgagaattt actatttcaa attaattcaa agtgacagta ttttttactt
    14701 atttggtttc aaattttgta atccttccta tttattggaa tacactccac attttgtata
    14761 caagtcatat catgttttag caaagctctt accaagcaaa gttataatga tgttttccaa
    14821 agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa aattagatgt
    14881 atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt tttttatt
//
LOCUS       DARBEG010000065        14815 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000065 DARBEG010000000
VERSION     DARBEG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.066"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(296..1024)
                     /locus_tag="SCK20_002007"
     CDS             complement(296..1024)
                     /locus_tag="SCK20_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG1146049.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="SCK20_002008"
     CDS             complement(1002..2576)
                     /locus_tag="SCK20_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1146050.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="SCK20_002009"
     CDS             2805..3743
                     /locus_tag="SCK20_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1146051.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="SCK20_002010"
     CDS             3755..4666
                     /locus_tag="SCK20_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1146052.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="SCK20_002011"
     CDS             4690..6162
                     /locus_tag="SCK20_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1146053.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="SCK20_002012"
     CDS             6213..6824
                     /locus_tag="SCK20_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1146054.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="SCK20_002013"
     CDS             6854..8767
                     /locus_tag="SCK20_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG1146055.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="SCK20_002014"
     CDS             8895..11876
                     /locus_tag="SCK20_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG1146056.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="SCK20_002015"
     CDS             11873..13534
                     /locus_tag="SCK20_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG1146057.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="SCK20_002016"
     CDS             13574..14506
                     /locus_tag="SCK20_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG1146058.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DARBEG010000066        14472 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000066 DARBEG010000000
VERSION     DARBEG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.067"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(277..639)
                     /locus_tag="SCK20_002017"
     CDS             complement(277..639)
                     /locus_tag="SCK20_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146059.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(713..1093)
                     /locus_tag="SCK20_002018"
     CDS             complement(713..1093)
                     /locus_tag="SCK20_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG1146060.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1157..2350
                     /locus_tag="SCK20_002019"
     CDS             1157..2350
                     /locus_tag="SCK20_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1146061.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2427..3011)
                     /locus_tag="SCK20_002020"
     CDS             complement(2427..3011)
                     /locus_tag="SCK20_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1146062.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3212..4210
                     /locus_tag="SCK20_002021"
     CDS             3212..4210
                     /locus_tag="SCK20_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1146063.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4322..4831
                     /locus_tag="SCK20_002022"
     CDS             4322..4831
                     /locus_tag="SCK20_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG1146064.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4861..5520)
                     /locus_tag="SCK20_002023"
     CDS             complement(4861..5520)
                     /locus_tag="SCK20_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG1146065.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5522..6286)
                     /locus_tag="SCK20_002024"
     CDS             complement(5522..6286)
                     /locus_tag="SCK20_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG1146066.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6294..6971)
                     /locus_tag="SCK20_002025"
     CDS             complement(6294..6971)
                     /locus_tag="SCK20_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG1146067.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7008..8393)
                     /locus_tag="SCK20_002026"
     CDS             complement(7008..8393)
                     /locus_tag="SCK20_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1146068.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8603..8687)
                     /gene="ffs"
                     /locus_tag="SCK20_002027"
     ncRNA           complement(8603..8687)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SCK20_002027"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="SCK20_002028"
     CDS             complement(8710..9225)
                     /gene="tadA"
                     /locus_tag="SCK20_002028"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG1146069.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9332..10189
                     /locus_tag="SCK20_002029"
     CDS             9332..10189
                     /locus_tag="SCK20_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146070.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10270..10605)
                     /locus_tag="SCK20_002030"
     CDS             complement(10270..10605)
                     /locus_tag="SCK20_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146071.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10672..12042)
                     /locus_tag="SCK20_002031"
     CDS             complement(10672..12042)
                     /locus_tag="SCK20_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG1146072.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12120..12656)
                     /locus_tag="SCK20_002032"
     CDS             complement(12120..12656)
                     /locus_tag="SCK20_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1146073.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12965..14446
                     /locus_tag="SCK20_002033"
     CDS             12965..14446
                     /locus_tag="SCK20_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146074.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4458 a         2883 c         2353 g         4778 t
ORIGIN      
        1 tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat
       61 ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta
      121 ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact
      181 atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa
      241 aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac
      301 ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc
      361 taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc
      421 tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat
      481 ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa
      541 cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa
      601 atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt
      661 gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct
      721 tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg
      781 caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg
      841 gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt
      901 catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat
      961 ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa
     1021 gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa
     1081 ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa
     1141 aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc
     1201 aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga
     1261 cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc
     1321 gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata
     1381 tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat
     1441 cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc
     1501 aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac
     1561 aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga
     1621 cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa
     1681 tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt
     1741 gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt
     1801 cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc
     1861 ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat
     1921 caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga
     1981 aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt
     2041 aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca
     2101 gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt
     2161 taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt
     2221 cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat
     2281 tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac
     2341 aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt
     2401 tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca
     2461 tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc
     2521 atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca
     2581 cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata
     2641 ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct
     2701 gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca
     2761 aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg
     2821 atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa
     2881 agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg
     2941 tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt
     3001 gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata
     3061 cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt
     3121 ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat
     3181 tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg
     3241 tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa
     3301 aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata
     3361 tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga
     3421 aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac
     3481 tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt
     3541 cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga
     3601 atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa
     3661 aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc
     3721 tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag
     3781 tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt
     3841 atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac
     3901 gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca
     3961 tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc
     4021 tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa
     4081 actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat
     4141 taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg
     4201 tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc
     4261 atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa
     4321 tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg
     4381 cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga
     4441 tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca
     4501 taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag
     4561 tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc
     4621 cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa
     4681 ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt
     4741 cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga
     4801 accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat
     4861 ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac
     4921 agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc
     4981 tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa
     5041 aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa
     5101 aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt
     5161 cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata
     5221 atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg
     5281 gaaatctggc aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc
     5341 aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc
     5401 gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag
     5461 tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat
     5521 attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag
     5581 gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta
     5641 tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca
     5701 gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg
     5761 gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc
     5821 cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa
     5881 tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag cctaccacta
     5941 catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc
     6001 ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg
     6061 ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta
     6121 gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta
     6181 attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac
     6241 gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct
     6301 tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt
     6361 ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt
     6421 ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt
     6481 ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa
     6541 tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga
     6601 tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa
     6661 ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc
     6721 ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc
     6781 aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc
     6841 tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca
     6901 gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt
     6961 tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg
     7021 tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca
     7081 ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta
     7141 gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta
     7201 atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc
     7261 attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag
     7321 cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca
     7381 gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg
     7441 tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata
     7501 aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt
     7561 tcttttaata attcatgccc acgttctaga taactattta tttcttgttc atcttctgta
     7621 aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct
     7681 tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt
     7741 tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata
     7801 acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc
     7861 atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca
     7921 taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat
     7981 gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat
     8041 gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca
     8101 tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc
     8161 gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct
     8221 tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt
     8281 ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa
     8341 ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct
     8401 ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc
     8461 tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag
     8521 tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac
     8581 ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc
     8641 ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa
     8701 tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg
     8761 gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc
     8821 tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt
     8881 tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc
     8941 gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca
     9001 gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc
     9061 ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac
     9121 tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg
     9181 catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc
     9241 tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc
     9301 tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta
     9361 cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat
     9421 tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt
     9481 gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc gctatctttt
     9541 taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc
     9601 aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc
     9661 tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga
     9721 ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac
     9781 gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt
     9841 tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac
     9901 tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa
     9961 gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga
    10021 attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa
    10081 tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc
    10141 caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa
    10201 aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc
    10261 tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt
    10321 tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc
    10381 taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa
    10441 tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat
    10501 atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc
    10561 ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt
    10621 tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa
    10681 tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac
    10741 aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc
    10801 tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat
    10861 cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc
    10921 taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac
    10981 ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt
    11041 tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg
    11101 tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg
    11161 tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct
    11221 tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac
    11281 tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca
    11341 agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc
    11401 ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg
    11461 atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa
    11521 gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac
    11581 tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt
    11641 cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc
    11701 cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat
    11761 tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac
    11821 agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt
    11881 ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct
    11941 tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt
    12001 tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac
    12061 attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc
    12121 taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc
    12181 caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc
    12241 aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg
    12301 acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg
    12361 atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa
    12421 acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat
    12481 atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa
    12541 ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat
    12601 accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc catcattctt
    12661 acctccgtct cttttcatct acccataaat aaataacata aataattcta aaaatattat
    12721 tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc cgccttaaaa
    12781 acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca aggtaacaaa
    12841 aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa cacagactga
    12901 aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata aaaaggaggt
    12961 atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg tcattcaaaa
    13021 aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac ggacaatcaa
    13081 agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg atgaatttat
    13141 ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta aaaagttagc
    13201 tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta actatttgat
    13261 gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg aacaacttta
    13321 tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct taaaaaaatt
    13381 ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg agttatctat
    13441 ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt ggccattttc
    13501 ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag aaaaaatttc
    13561 aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa acaaacaaca
    13621 ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat tgcagcatac
    13681 acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg aagagacaga
    13741 aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc aaatctcttt
    13801 tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta tttgtcgaca
    13861 agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt tagaaaataa
    13921 aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac tgttgggtga
    13981 atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc attctagttc
    14041 ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa aaaatgggga
    14101 aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac aaacagaaac
    14161 gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa attatcatct
    14221 tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg catattcaga
    14281 acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa cggagacttt
    14341 ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag cggttcatga
    14401 actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa attcttgtca
    14461 catcctctta tt
//
LOCUS       DARBEG010000067        14344 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000067 DARBEG010000000
VERSION     DARBEG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.068"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            110..491
                     /locus_tag="SCK20_002034"
                     /pseudo
     CDS             110..491
                     /locus_tag="SCK20_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1572
                     /locus_tag="SCK20_002035"
     CDS             622..1572
                     /locus_tag="SCK20_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1146075.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2545
                     /locus_tag="SCK20_002036"
     CDS             2090..2545
                     /locus_tag="SCK20_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1146076.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2886..3488
                     /locus_tag="SCK20_002037"
     CDS             2886..3488
                     /locus_tag="SCK20_002037"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146077.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3582..3914
                     /locus_tag="SCK20_002038"
     CDS             3582..3914
                     /locus_tag="SCK20_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG1146078.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(4065..5044)
                     /locus_tag="SCK20_002039"
                     /pseudo
     CDS             complement(4065..5044)
                     /locus_tag="SCK20_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5049..5816)
                     /locus_tag="SCK20_002040"
     CDS             complement(5049..5816)
                     /locus_tag="SCK20_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1146079.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6306..6731
                     /gene="lacA"
                     /locus_tag="SCK20_002041"
     CDS             6306..6731
                     /gene="lacA"
                     /locus_tag="SCK20_002041"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEG1146080.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6748..7263
                     /gene="lacB"
                     /locus_tag="SCK20_002042"
     CDS             6748..7263
                     /gene="lacB"
                     /locus_tag="SCK20_002042"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEG1146081.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7274..8206
                     /gene="lacC"
                     /locus_tag="SCK20_002043"
     CDS             7274..8206
                     /gene="lacC"
                     /locus_tag="SCK20_002043"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEG1146082.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8209..9189
                     /gene="lacD"
                     /locus_tag="SCK20_002044"
     CDS             8209..9189
                     /gene="lacD"
                     /locus_tag="SCK20_002044"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1146083.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9217..9534
                     /locus_tag="SCK20_002045"
     CDS             9217..9534
                     /locus_tag="SCK20_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1146084.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9534..11240
                     /locus_tag="SCK20_002046"
     CDS             9534..11240
                     /locus_tag="SCK20_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1146085.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11382..12788
                     /gene="lacG"
                     /locus_tag="SCK20_002047"
     CDS             11382..12788
                     /gene="lacG"
                     /locus_tag="SCK20_002047"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1146086.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12811..13083
                     /locus_tag="SCK20_002048"
     CDS             12811..13083
                     /locus_tag="SCK20_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEG1146087.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13104..14003
                     /locus_tag="SCK20_002049"
     CDS             13104..14003
                     /locus_tag="SCK20_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1146088.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4686 a         2363 c         2765 g         4530 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaatatg tttattaaaa
     4261 ctaagtttat tatcaatatc aattcctgct cgttttggat taccttttcg atttaatttt
     4321 ccattttcag gtgagttgag gaaattgtta aggacactat aatgaagagg tgttccatta
     4381 tagtcgggtt tatgacgact tttggaagta aaaatccatc cattatcttt ataatcagga
     4441 ttgaatctca tatgttggtg atttaactca atttcctctt taagaatttc tatagccctt
     4501 ttagataatt ggacatttcg gtaactttgt aagtttttta cattaccttc atagctattt
     4561 tctgcagctc ctccgtgagt ggactgacta atgatgatat tttgcttttc aaaatattga
     4621 taggcaccaa tattttggag tttcgactct gtaagtcaac agggccatta gttagaatac
     4681 ctattgggat gcggttttta tgtagagcca gcagtagttg atgtatctca ggaatcatcg
     4741 taatctgatc gagttcatat tcatagtcag cttggaagtc tagagcttct gtcctagaaa
     4801 tactatcgat gccaaattct tccatagtct tctgacagcg aaaaattcta agctgctcaa
     4861 tactgatgag ttttttcgtg tatttaggaa aagcaacatc agaccaatat ctgaaacgtt
     4921 tgtaaatgtt atctatttgt tggatatcaa tcgttggaaa acatttctca acgctacgtt
     4981 taaaggggag ttgatggtca taaagagtgt catcaaggtc aaaaacaata tttttataag
     5041 tcataattct agtccgcttt taaaatagta gtgtactggc taaattcctc taagtgttga
     5101 gggctaatct ggttatctgt aatgatggta tcgagttgat ctagattata gaagttaaag
     5161 aaatcgtatc gatcgaattt agtactgtct actaataaga atttttctac agcattgttt
     5221 agggcaagtt gttgaatcac accttccttg tcactatatg tagcaataga attatgggta
     5281 acagcatttg cactaacaaa agctttggca aatctaattg cttttaaatt tgtcgaagcc
     5341 attgaaccta caaaagctcc agttatttct ctatattcac cgccaagaag caataaatca
     5401 atggtttcgc tatcatttag aatcaagaac acagggagac tatttgtaat gacacgaatt
     5461 ttataacctt tacgaccttt caattctaat gccagttgtt caagtgtagt tccaggtcca
     5521 ataaaaatag tatcgccatc tttgattaaa gagcaggctt ttttagcaat gtcaattttt
     5581 tcttttgtat ttagactttt cttctcgata tgtgtctttt caagtggctt tttagaggaa
     5641 agcaattgtg caccaccatg agtacgtgtt aaaagtccat cagcttctag agcatcaagg
     5701 tctctacggg ctgtcatatc ggaaatatct agttcatcta ttatttcttt tatggttatg
     5761 gtcccatcaa tctttaacat atctaaaatt ttttctaatc gtcgtttttt gttcatatga
     5821 agacccttct ttcataaagt aatttttttc caaagataat tctcttttaa ttgtatcata
     5881 aaagacaata ttttcaaggt aaaacaaaca atttcaaaca aaaacaaatg ttagatgatg
     5941 aaataagaac agaggattga cgtatattag cttaggtcag attttgtata agacgaaaat
     6001 aaagtaggac ctcttaatca gtaagttata gaaagtaaaa gacttttgta atacttgaat
     6061 agatatttca cgtccatttt gtgatggatt aaatgaacaa aaatgaacaa taatttaacg
     6121 gtgttatcta ttttttaaaa aaacaaataa aaaaaacaaa aaataaacaa aaaaagtgtt
     6181 gctttttgtt tgaatgcttg atattatata aacaaagaaa tgatgaaaac gttttttcga
     6241 acattttgca aaatgttttc tacttctacg tagcatttct ttctaaaatt taggaggtgg
     6301 tcaaaatggc tattgttgtt ggtgcagatc tcaaaggtac gagattaaaa gatgttgtga
     6361 aaaattttct tgtagaagaa ggttttgagg taattgatgt gactaaggac ggacaagatt
     6421 ttgttgatgt taccttggca gttgcctctg aagtaaataa agatgagcaa aacttaggta
     6481 ttgtaattga tgcttatgga gctggtccat tcatggtagc aactaaaatt aaaggtatgg
     6541 ttgcagcaga agtttcagat gaacgctcag cttatatgac acgtggacat aacaattcac
     6601 gaatgattac ggttggcgct gaaatcgtcg gtgatgagct tgctaaaaac atcgctaaag
     6661 gtttcgtcaa tggtaaatat gatggcggac gtcatcaaat ccgagtagat atgcttaaca
     6721 aaatgtgtta agaaaaggag ttaaggaatg agaatcgcaa ttggatgtga ccacattgtc
     6781 acagatgaaa aaatggcagt atcagaattc ttgaaatcaa aaggacatga ggtccttgat
     6841 ttcgggacat atgaccacgc acgtactcac taccctatct atggtaaaaa agtcggtgaa
     6901 gcagtagtaa gtggtcaagc agacttggga gtatgtatct gtggtacagg tgtcggcatt
     6961 aacaatgcag tcaataaagt tccaggtgtt cgctcagcgt tggttcgtga tatgacatca
     7021 gctatatatg caaaagaaga gttaaatgcg aatgttatcg gtttcggtgg aaagattact
     7081 ggtggtcttc ttatgaatga catcattgaa gctttcattg aagctgagta caaaccaaca
     7141 gaagaaaata aaaaattgat tgcaaaaatc gaacacgttg aaacacataa tgcacatcaa
     7201 gcagatgaag atttctttac agaattcctt gaaagatggg accgtggtga ctaccacgat
     7261 taaggtataa caaatgattc tgacagtcac actaaatcct tcagtagaca tctcttatcc
     7321 tctagaaaca ttgaaaattg atacagtgaa tagggtgaaa gatgttagta agacagctgg
     7381 tggaaaaggc ttaaatgtta ctagagtctt gtatgaatct ggtgataaag ttactgctac
     7441 aggtttctta ggtggtaaaa ttggtgaatt tattgaaagc gaattagaac aatctcctgt
     7501 tagtccagct ttttataaaa tctcaggaaa cactagaaac tgtattgcta tcttacacga
     7561 gggtaaccaa acagaaattt tggaacaagg accaacaatt tctcatgaag aagcagaagg
     7621 ctttcttgac cactatagca atcttattaa acaatcagaa gttgtgacaa tttcaggaag
     7681 tttaccttca ggacttccca acgattatta tgaaaaactt atccaacttg cttcggatga
     7741 aggagtagca gtcgttttgg attgttcagg tgcacctctt gaaacagttc taaaatcgtc
     7801 ggctaaacct actgccatca aacctaataa tgaggaactt tcacagttgc ttggaaaaga
     7861 agtaacaaaa gatattgaag aactcaaaga cgttcttaaa gagtcacttt ttagtggtat
     7921 cgaatggata gtggtctcat taggtcgaaa cggtgccttt gcaaaacatg gtgatgtttt
     7981 ctataaggta gatattcctg atatcccagt tgttaatcca gtcggatcag gggactcaac
     8041 ggtagctggt attgcatcag ctttaaatag taaaaaaagt gacgctgact tattaaaaca
     8101 tgcgatgaca ttgggtatgt taaatgctca agaaacaatg acagggcatg tgaatatgac
     8161 taattacgaa acactaaact cacaaattgg agtaaaagag gtataaaaat ggtacttaca
     8221 gaacagaaac gcaaatcttt ggaaaaaatt tcagataaaa acggtgttat ctcagctttg
     8281 gcatttgacc aacgtggtgc tttgaaacgt ttgatggcac agtatcaaga tacagaacca
     8341 actgtagctc aaatggaaga actaaaggtt ttggttgctg atgaacttac aaaatacgct
     8401 tcttcaatgt tgcttgaccc tgaatatgga cttccagcaa caaaagcatt ggataaagaa
     8461 gcaggtcttc ttcttgccta tgaaaaaaca ggttatgata catcaagtac aaaacgtttg
     8521 cctgactgct tggatgtttg gtctgcaaaa cgtatcaaag agcaaggcgc tgatgccgtt
     8581 aagttcttgc tttactatga tgtagacagc tcagatgaac ttaaccaaca aaaacaagct
     8641 tatattgaac gtgttggttc tgaatgtgtc gctgaagata ttcctttctt cttggaaatc
     8701 cttgcttatg atgaagaaat ctcagatgca ggttctgttg aatatgcaaa agtgaaacct
     8761 cgcaaagtca ttgaagcgat gaaagttttc tctgatccac gctttaacat tgatgttctt
     8821 aaagtagaag taccagttaa cgttaaatat gtcgaaggtt ttgctgatgg tgaagtagtt
     8881 tacagtaaag ctgaagctgc tgacttcttt aaagcacaag aagaagcgac taaccttcca
     8941 tacatctacc taagtgcagg tgtatctgct aaattgttcc aagaaacatt gcaatttgct
     9001 cacgactcag gtgccaagtt taatggtgtg ctttgtggac gtgctacttg ggcaggatct
     9061 gtagaacctt acatcaaaga aggtgaaaaa gcagcacgtg aatggttgcg tactactgga
     9121 ttcgaaaata ttgatgaact taacaaagtt cttgttaaaa cagctagtcc atggactgat
     9181 aaagtatagt aaaaacataa aacggaggat attgttatga acagagaaga gatgactctc
     9241 ttagggtttg aaattgttgc ttatgctgga gatgctcgct ctaaactttt agaagcgctt
     9301 aaagcggctg aaaatggtga tttcgctaag gcagatagtc ttgtagtaga agcaggaagc
     9361 tgtattgcag aggctcacaa ttctcagaca gctatgttgg ctcgagaagc ttctggggag
     9421 gaacttccat acagcgttac catgatgcat ggtcaggacc acttaatgac tactgtctta
     9481 ttaaaagatg tgattcatca cctcatcgaa ctttataaaa gaggagcaaa ataatgcata
     9541 aactcattga acttattgag aaagggaaac cgttctttga gaaaatctct cgaaacatct
     9601 atcttcgtgc tattcgtgat ggatttatcg ctggtatgcc agtcatcctt ttctcatcta
     9661 tctttatcct tattgcctat gtcccaaatg cttggggatt ccactggtca aaagacattg
     9721 agaccttctt gatgactcct tatagctatt cgatgggtat cctagcattc tttgttggtg
     9781 gtactaccgc taaagctttg acggactcaa tgaaccgtga cctacctgcg accaatcaga
     9841 ttaacttctt atctacgatg ctagcgtcta tggtagggtt ccttttgatg gcagctgagc
     9901 ctgcaaaaga aggaggcttc ttgacagcct tcacgggaac aaaaggtctt ttgacagcat
     9961 ttattgcagc ctttgtgaca gttaatgttt acaaggtctg tgtgaaaaat aatgttacta
    10021 ttcgtatgcc tgaagaggtt ccaccaaata tctctcaagt atttaaagac ttgattccat
    10081 tcactgtagc ggttgttctc ctttatggat ttgaactcat tgttaaggga actcttggtg
    10141 taaccgttgc agaatcaatc ggtacccttc ttgctcctct cttctcagct gcagatggtt
    10201 accttggaat tacacttatc tttggtgctt atgccttctt ctggtttgtt ggtatccacg
    10261 gtccttcaat tgtcgaacca gcaatcgctg ctatcactta tgccaatatc gatgccaact
    10321 tgcatcttat ccaagctgga caacatgcag acaaagttat cacttctggt actcaaatgt
    10381 ttattgttac catgggtgga acaggagcta cattgattgt tccattcttg ttcatgtgga
    10441 tttgtaaatc agaacgtaac cgtgccatcg gacgtgcctc agttgttcca acattctttg
    10501 gggttaatga gccaatctta tttggtgcac caatcgtatt gaatccgatt ttctttgtac
    10561 cgttcatttt cgctccgatt gtcaacgttt ggatctttaa attctttgtt gacacattga
    10621 acatgaactc attctctgcc aaccttccat gggttactcc tggtccgttg ggaattgtac
    10681 tcggtactaa cttccaagtt ctatcattta tcttagccgg actcttggta gttgttgata
    10741 ctatcattta ttacccattt gttaaggtat acgatgaaca aattcttgaa gaagaacgtt
    10801 ctggtaaaac aaacgatgct cttaaagaaa aagtagctgc aaacttcaat actgctaaag
    10861 cagatgccgt tcttggaaaa gcaggcgttg ctaaagaaga tgtagcagca aacaataata
    10921 tcacaaaaga aactaatgtc cttgttcttt gtgcaggtgg aggtacaagt ggcttgcttg
    10981 ccaatgcctt gaacaaagca gcagcagaat acaatgttcc agtcaaagcg gcagcgggta
    11041 gttacggtgc tcaccgtgaa atgttgccag agtttgactt ggttatcttg gctccacagg
    11101 tcgcttcaaa cttcgatgat atgaaggctg aaacggataa attgggcatt aaacttgcaa
    11161 aaactgaagg agctcaatat atcaaattga cacgtgatgg acaaggtgct cttgcatttg
    11221 ttcaacaaca gtttgattaa taaatttgta aaagttattt gagacgagat tattctttca
    11281 attccttata atattgttaa ccatatcatt gcatttcatt tccttggttt ccggaactca
    11341 gaatatcgtc tcaaatgctt tttttgaaag gatttatagt tatgactaaa acacttccta
    11401 aagattttat ttttggtggt gctacagctg cttatcaagc agaaggtgcg actcacacgg
    11461 atggtaaagg cccagttgca tgggataagt atcttgaaga caattattgg tacacagcag
    11521 agcctgcaag tgatttttac cataaatatc ctgttgattt agaattagct gaaaaatatg
    11581 gcgttaatgg tattcgtatt tcaattgcct ggtctcgtat tttcccaact ggctatggcg
    11641 aagtaaatga aaaaggtgtt gaattctacc ataatctttt tgccgagtgc cacaaacgtc
    11701 atgttgagcc attcgtgact ttgcaccact ttgacacacc agaagttctt cactcagatg
    11761 gagatttctt aaaccgcgaa aatatagaac actttgtaga ttatgccgct ttctgttttg
    11821 aagagttccc agaagtaaac tactggacaa ctttcaatga aattggccca attggagatg
    11881 gtcaatactt ggttggtaaa ttccctccag gcattcaata tgatcttgcc aaagtcttcc
    11941 aatcacacca caatatgatg gtttctcatg cacgtgctgt gaaattgtat aaagataaag
    12001 gttataaagg cgaaattggt gtagtacatg cgttgccaac caaataccct tatgatccag
    12061 aaaatccagc agatgttcgt gccgctgagt tagaagatat catccataac aaatttatct
    12121 tggatgcaac ctaccttgga cactattcag ataaaacgat ggaaggggtc aaccacatcc
    12181 tagctgaaaa tggtggagaa cttgatcttc gtgatgaaga cttccaagca cttgaagcag
    12241 ctaaagattt gaacgatttc cttggtatca actactacat gagtgattgg atgcaagcct
    12301 ttgatggtga gactgaaatc attcacaatg gtaagggtga aaaaggaagt tctaagtacc
    12361 agattaaagg tgttggtcgc cgaatcgcac cagattatgt gcctagaacg gattgggact
    12421 ggattattta tcctgaaggc ttgtatgacc aaatcatgcg agtgaaaaat gattatccaa
    12481 actacaagaa gatttacatc actgaaaacg gtcttggata caaagatgag tttgtagata
    12541 ataccgttta tgatgatggt cgtattgatt acgtgaagaa acacttggaa gttttatcag
    12601 acgcaattgc ggatggtgca aatgttaaag gttacttcat ctggtcactt atggatgtct
    12661 tctcatggtc aaacggatac gaaaaacgtt atggactatt ctatgtagac tttgacacgc
    12721 aagaacgcta tcctaagaaa tcagctcatt ggtataagaa agtagcagaa actcaagtga
    12781 tagagtaata ttacttgaaa atgtaaaaaa ttgttatccg atactttaga aaaagtacaa
    12841 cacttattta gaccaggctt tgaaaaggta tatattgtta gtttcgaaga ttgtcctatg
    12901 attcctgagc ttgaagctac cccgttgcta aaatgtggta aatggtatgt ttcaactgga
    12961 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
    13021 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
    13081 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
    13141 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
    13201 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
    13261 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacccttt tttacaggtc
    13321 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
    13381 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
    13441 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
    13501 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaata
    13561 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
    13621 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
    13681 cttttttgga aaatcaaaaa attctcgatt tggactactc acttttttcc catgatgcca
    13741 ttacacttga ccagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
    13801 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
    13861 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
    13921 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
    13981 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agatttgaat
    14041 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
    14101 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaat atctaaagac
    14161 agtctggtag taaaaaataa gattatttac tacttcttga tttaaaataa tagtggagcc
    14221 attaatttgt ggcaaattta atttaatatt ttgcctcaaa ttagccacac tttttataga
    14281 actaagagga aaaatgaaat tttcttccta ttaatatagt tgtttgaaat cttaaatata
    14341 gcgt
//
LOCUS       DARBEG010000068        13943 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000068 DARBEG010000000
VERSION     DARBEG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.069"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            136..309
                     /locus_tag="SCK20_002050"
     CDS             136..309
                     /locus_tag="SCK20_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146089.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="SCK20_002051"
     CDS             949..1422
                     /locus_tag="SCK20_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG1146090.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="SCK20_002052"
     CDS             1431..1658
                     /locus_tag="SCK20_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1146091.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="SCK20_002053"
     CDS             2294..2665
                     /locus_tag="SCK20_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146092.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="SCK20_002054"
     CDS             complement(2921..3163)
                     /locus_tag="SCK20_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1146093.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="SCK20_002055"
     CDS             complement(3195..4097)
                     /locus_tag="SCK20_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1146094.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="SCK20_002056"
     CDS             complement(4110..4298)
                     /locus_tag="SCK20_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1146095.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="SCK20_002057"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="SCK20_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1146096.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="SCK20_002058"
     CDS             complement(4913..5806)
                     /locus_tag="SCK20_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1146097.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="SCK20_002059"
     CDS             complement(5884..6807)
                     /locus_tag="SCK20_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1146098.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="SCK20_002060"
     CDS             complement(6856..7206)
                     /locus_tag="SCK20_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1146099.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="SCK20_002061"
     CDS             complement(7239..8141)
                     /locus_tag="SCK20_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1146100.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="SCK20_002062"
     CDS             complement(8134..8991)
                     /locus_tag="SCK20_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1146101.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="SCK20_002063"
     CDS             9325..10134
                     /locus_tag="SCK20_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1146102.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="SCK20_002064"
     CDS             10174..10671
                     /locus_tag="SCK20_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1146103.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="SCK20_002065"
     tRNA            complement(11056..11129)
                     /locus_tag="SCK20_002065"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="SCK20_002066"
     CDS             11316..11639
                     /locus_tag="SCK20_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146104.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="SCK20_002067"
     CDS             11803..12057
                     /locus_tag="SCK20_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146105.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="SCK20_002068"
     CDS             12127..12372
                     /locus_tag="SCK20_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1146106.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="SCK20_002069"
     CDS             12448..12813
                     /locus_tag="SCK20_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146107.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12874..13437)
                     /locus_tag="SCK20_002070"
     CDS             complement(12874..13437)
                     /locus_tag="SCK20_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146108.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         4087 a         2577 c         2304 g         4975 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tattatgcct tttttaatat ataacacagt
    13441 tatttttaaa aataagaatg ctaaatcact ctacaataac ggcgaaagca caaaaaataa
    13501 aaaaattatt acgttattaa aaaaacagat gaaagatcag gttttattag atctcattct
    13561 ttcatcactt gaagggattt aatgaactac tttttcctct ttgtcgtttt tatatattcg
    13621 tattccttca aacagtagga tagcctcttt caatgcttga tatttgaact gtccgttact
    13681 taaaaattta gatgtgccgc cctcttccta gaacatattc ttttttactt tttcattaat
    13741 tgacagacca atcgaacgaa actgtaaatt tcgataggat aatgattggt caaactcatt
    13801 cattttcaag acatctcgat tcatattcaa gataggaatc tttttatcaa catcacttta
    13861 ttcgtatttt cctctaccat aaaagcttca cgctccacaa aattgtttta tacactacta
    13921 ttcagcccat tcaacgacca atg
//
LOCUS       DARBEG010000069        13925 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000069 DARBEG010000000
VERSION     DARBEG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.070"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            206..544
                     /locus_tag="SCK20_002071"
     CDS             206..544
                     /locus_tag="SCK20_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146109.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(749..1324)
                     /locus_tag="SCK20_002072"
     CDS             complement(749..1324)
                     /locus_tag="SCK20_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG1146110.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2056..2637)
                     /locus_tag="SCK20_002073"
     CDS             complement(2056..2637)
                     /locus_tag="SCK20_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1146111.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2820..3446)
                     /locus_tag="SCK20_002074"
     CDS             complement(2820..3446)
                     /locus_tag="SCK20_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1146112.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3468..4799)
                     /locus_tag="SCK20_002075"
     CDS             complement(3468..4799)
                     /locus_tag="SCK20_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG1146113.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4954..5355)
                     /locus_tag="SCK20_002076"
     CDS             complement(4954..5355)
                     /locus_tag="SCK20_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1146114.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5441..5647)
                     /locus_tag="SCK20_002077"
     CDS             complement(5441..5647)
                     /locus_tag="SCK20_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146115.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5742..7244)
                     /locus_tag="SCK20_002078"
     CDS             complement(5742..7244)
                     /locus_tag="SCK20_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG1146116.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7262..7717)
                     /gene="argR"
                     /locus_tag="SCK20_002079"
     CDS             complement(7262..7717)
                     /gene="argR"
                     /locus_tag="SCK20_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1146117.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7815..8432)
                     /locus_tag="SCK20_002080"
     CDS             complement(7815..8432)
                     /locus_tag="SCK20_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG1146118.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8653..10026
                     /locus_tag="SCK20_002081"
     CDS             8653..10026
                     /locus_tag="SCK20_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1146119.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10101..11213
                     /locus_tag="SCK20_002082"
     CDS             10101..11213
                     /locus_tag="SCK20_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG1146120.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11285..12922)
                     /locus_tag="SCK20_002083"
     CDS             complement(11285..12922)
                     /locus_tag="SCK20_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG1146121.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13090..13698)
                     /locus_tag="SCK20_002084"
     CDS             complement(13090..13698)
                     /locus_tag="SCK20_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1146122.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3780 a         2909 c         2483 g         4753 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      121 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      181 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      241 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      301 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      361 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      421 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      481 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      541 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      601 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      661 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      721 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      781 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      841 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      901 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      961 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1021 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1081 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1141 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1201 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1261 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1321 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1381 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1441 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1501 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1561 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     1621 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     1681 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     1741 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     1801 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     1861 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     1921 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     1981 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2041 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2101 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2161 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2221 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     2281 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     2341 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     2401 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     2461 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     2521 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     2581 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     2641 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     2701 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     2761 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     2821 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     2881 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     2941 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3001 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3061 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3121 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3181 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3241 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     3301 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     3361 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     3421 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     3481 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     3541 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     3601 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     3661 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     3721 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     3781 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     3841 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     3901 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     3961 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4021 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4081 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4141 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4201 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4261 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     4321 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     4381 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     4441 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     4501 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     4561 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     4621 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     4681 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     4741 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     4801 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     4861 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     4921 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     4981 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5041 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5101 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5161 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5221 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     5281 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     5341 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     5401 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     5461 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     5521 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     5581 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     5641 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     5701 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     5761 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     5821 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     5881 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     5941 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6001 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6061 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6121 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6181 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6241 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     6301 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     6361 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     6421 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     6481 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     6541 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     6601 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     6661 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     6721 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     6781 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     6841 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     6901 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     6961 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7021 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7081 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7141 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7201 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7261 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     7321 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     7381 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     7441 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     7501 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     7561 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     7621 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     7681 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     7741 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     7801 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     7861 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     7921 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     7981 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8041 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8101 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8161 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8221 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     8281 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     8341 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     8401 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     8461 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     8521 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     8581 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     8641 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     8701 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     8761 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     8821 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     8881 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     8941 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9001 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9061 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9121 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9181 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9241 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
     9301 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
     9361 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
     9421 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
     9481 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
     9541 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
     9601 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
     9661 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
     9721 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
     9781 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
     9841 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
     9901 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
     9961 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10021 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10081 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10141 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10201 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10261 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    10321 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    10381 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    10441 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    10501 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    10561 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    10621 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    10681 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    10741 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    10801 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    10861 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    10921 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    10981 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11041 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11101 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11161 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11221 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    11281 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    11341 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    11401 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    11461 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    11521 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    11581 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    11641 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    11701 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    11761 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    11821 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    11881 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    11941 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12001 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12061 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12121 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12181 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12241 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    12301 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    12361 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    12421 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    12481 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    12541 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    12601 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    12661 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    12721 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    12781 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    12841 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    12901 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    12961 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13021 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13081 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13141 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13201 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13261 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    13321 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    13381 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    13441 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    13501 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    13561 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    13621 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    13681 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    13741 attaacttta tcatttagaa atatttcata ggtaaaagaa acatgacatt taatgacaaa
    13801 ataacgaatt atttaatatt tgtaacattt aaacaaatat ttttttgata caaaaccata
    13861 aaagccttgt tgtataagca aagtaatcgt ttttctatgt ttttttattt ttgaatgaaa
    13921 aacgc
//
LOCUS       DARBEG010000070        13810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000070 DARBEG010000000
VERSION     DARBEG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.071"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SCK20_002085"
     CDS             complement(221..1423)
                     /locus_tag="SCK20_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1146123.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SCK20_002086"
     CDS             complement(1426..1620)
                     /locus_tag="SCK20_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1146124.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SCK20_002087"
     CDS             complement(1667..2515)
                     /locus_tag="SCK20_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG1146125.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SCK20_002088"
     CDS             complement(2720..3070)
                     /locus_tag="SCK20_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146126.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SCK20_002089"
     CDS             3665..4111
                     /locus_tag="SCK20_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1146127.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SCK20_002090"
     CDS             4735..5727
                     /locus_tag="SCK20_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1146128.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SCK20_002091"
     CDS             5743..6627
                     /locus_tag="SCK20_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1146129.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SCK20_002092"
     CDS             6676..8283
                     /locus_tag="SCK20_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1146130.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SCK20_002093"
     CDS             8284..9768
                     /locus_tag="SCK20_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG1146131.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SCK20_002094"
     CDS             9945..10937
                     /locus_tag="SCK20_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1146132.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SCK20_002095"
     CDS             10989..11858
                     /locus_tag="SCK20_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1146133.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCK20_002096"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCK20_002096"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1146134.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DARBEG010000071        13480 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000071 DARBEG010000000
VERSION     DARBEG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.072"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1744)
                     /locus_tag="SCK20_002097"
     CDS             complement(<1..1744)
                     /locus_tag="SCK20_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG1146135.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYC"
     gene            complement(1757..2551)
                     /locus_tag="SCK20_002098"
     CDS             complement(1757..2551)
                     /locus_tag="SCK20_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1146136.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            complement(2562..3938)
                     /locus_tag="SCK20_002099"
     CDS             complement(2562..3938)
                     /locus_tag="SCK20_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1146137.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            complement(4067..4714)
                     /locus_tag="SCK20_002100"
     CDS             complement(4067..4714)
                     /locus_tag="SCK20_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1146138.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(4798..6225)
                     /gene="uxaC"
                     /locus_tag="SCK20_002101"
     CDS             complement(4798..6225)
                     /gene="uxaC"
                     /locus_tag="SCK20_002101"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1146139.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(6299..7378)
                     /gene="uxuA"
                     /locus_tag="SCK20_002102"
     CDS             complement(6299..7378)
                     /gene="uxuA"
                     /locus_tag="SCK20_002102"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1146140.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(7381..9012)
                     /locus_tag="SCK20_002103"
     CDS             complement(7381..9012)
                     /locus_tag="SCK20_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1146141.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            9329..10000
                     /locus_tag="SCK20_002104"
     CDS             9329..10000
                     /locus_tag="SCK20_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1146142.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(10836..11792)
                     /locus_tag="SCK20_002105"
     CDS             complement(10836..11792)
                     /locus_tag="SCK20_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146143.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(11901..12146)
                     /locus_tag="SCK20_002106"
                     /pseudo
     CDS             complement(11901..12146)
                     /locus_tag="SCK20_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12291..12713)
                     /locus_tag="SCK20_002107"
     CDS             complement(12291..12713)
                     /locus_tag="SCK20_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146144.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(12893..13264)
                     /locus_tag="SCK20_002108"
     CDS             complement(12893..13264)
                     /locus_tag="SCK20_002108"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1146145.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         4271 a         2733 c         1883 g         4593 t
ORIGIN      
        1 cacaataagc atctattgga cttattccct taaattcaga aaggcccatt agtttgtcca
       61 ctaatatttc taaagtttct tgttttccat cataagtatt gatataagtt tttacttgag
      121 gcacatcagc taaatgaaac ggacactgta cagatacaaa aattgttgga atttcatgaa
      181 tataaaatgg aatatccgga gtacctttac ttgctggcca aacaattctt tgaactgtac
      241 ctgggttaac attagctata ttaataatca agtcatattt ttcagttaaa tctttgattg
      301 gtctcttagc agcatatatt ccagctatat ttttaatttt ttcttcttgc gataatcctt
      361 tcaattcttc ttcaggtgaa atgtattttt caacttcaaa tccttttgct gacaaaatct
      421 gtttcaataa atcaatggcc tgaggtctat ttccagcaac tacttcacca aacccacctt
      481 ttaccccatg tacatctacc agtaaaatcc ttttatattt ttctggtaca ataggccaaa
      541 tatcttgttt atctttaact agtgtaattg cttgatcagc tacctcttta aaaattgatt
      601 tattttcaat taaaccaatt ttttccatag cttcttgttt cggtaacaaa attttattct
      661 tttctttttt atgcaaacct agtttagcct ttaagcctaa aattcttctt aatgcatcac
      721 gtaaacgctc ttctgaaata attcctttgt aataaccttc catcatataa ttaaagtctt
      781 catctggatc attaaaaaat aggaatagat cacatccagc cgcaatagaa gtgggcaata
      841 attctgaccg tttcattgcc ccagtcattc ctaacatatg actagcatca gtaactacta
      901 atccattaaa cccgagtttt cctctaagta agtcagtgat taattctttt gataaagttg
      961 caggaagtaa atcttcatct ttcagatcaa gattaaaatg ttttgaataa gctggcatat
     1021 ggatatgacc tgccataatt gaaggtaatc cagcttctat taagcctgag tatacttttc
     1081 caaaagaatt atcccattct tctgtactta aactattaat actaaaagat agatgttgat
     1141 ctcgttcgtc aataccgtca ccagggaaat gtttagctgc aggcaaaatt ccactctctt
     1201 gaataccttt catataagct aaactaagtt ctaaaacttt atcagggctt tgtccaaaag
     1261 atcgactaga aatgatagga tttctccaat ttctattaat atctacaata ggagcaaaac
     1321 tccagttaca tccaatagcc gatgcttcaa ttccagaaac tctccctaat tcataagcat
     1381 atttcgcatc atttgttgca ccaattttta cttctacacc aacttcggta ccatctgtac
     1441 aagctccgtt accacctgct tctgtattag cagcaattaa taaaggaatt ttgctttctg
     1501 tttgtaggat atagttttga tcatatacct catcagcagt tcctggatta tatcgtacag
     1561 ctccaatatg gtactgattg accatactac ttaagtaatt tttatcacga ctagatccca
     1621 tattaacaaa taattgacct attttttctt ctaaagtcat agcatcaatc gtctcttcaa
     1681 tccaagaaat agcatcttca tctaaatgat atggtttttt tcgtaagtct acttttatag
     1741 tcattttctt tctcctctac ttattagtaa gatttttcca aaaagtatct atatattttt
     1801 gttcaatatc tatatactct cccgataaac atttaaataa aatatcagtt cttaagtttt
     1861 cccatgattc tttttcttta ttaaccaaaa taggataaaa gaaaacttta ttcttatttg
     1921 cagcttctag atcaccagga gaatcaccaa tcattaatat cttatccaaa tcacaaccat
     1981 tttcaattac acttcgaata atgtcttctt tttttccttt atcttgacaa cacagttcat
     2041 caactaagga taataattca tgtctttccc attcttcttg aatagcttcc ttattagctg
     2101 agcttacaat aattatcttc ccatattttt tcagagcttt tatagtatct ctagcactta
     2161 aaaagggttt atctaatcct ttagcttgac taatctgtcg attgacttct tccgaccatt
     2221 ttaaagcttt taataaatcc tctgactgag agtattctat ttccctcttc aaagatggat
     2281 tagacaattc ttttgttaca gatacccagt gctttaatcg attaatcgat ggtacatcat
     2341 ttttttctaa tgttttaact aaacctaaaa atctgtttat tccacgagtc tctgaatata
     2401 aattaatatt attccaatcc tgtaggaatt tttccctatg atgaacatca aaaatctctg
     2461 cagcaattgg cccgaaaaag agattgtgtt tataagtcat tgtatccatg acacaaccat
     2521 ctgaatcaat acaaaatatc caactgttca tgttttttct cctagttatg tttttgattt
     2581 agcaaattat aattttctaa tactttttct ttagataatg gatatacgaa aaataaaatc
     2641 aatcctacaa caaagaaaca aactgctgga atcaacgttg aagagttata cagcttttgt
     2701 aaaacttctc cactttgtac ttttgctaac gaatcatacc ctattataga aagagtccaa
     2761 cctccgacac cgccagctaa agcttgtcca atttttcggg caaaagaata tactgcataa
     2821 atagttccat cttctctttg gccagatttc acttcttgat catcgattac atcagtaata
     2881 ttcgcccaaa tgactgtatt aaaaatacct aaccccatat atccaacaga agtaagaact
     2941 aagaatacat acatattact aggctttatc aaaaataaaa gtaaatatac aatagcagag
     3001 aagaacatcc ctactgacgc caattctttt ttaccgtatt ttttactgaa aattaaactt
     3061 acaggtgaaa caagtaccaa catcaaaata ggattgataa agttcattaa aactattccc
     3121 tgtgcattat tataataatc tggaaataca taattgttca tactcattgt taaaagctga
     3181 gcaagcaaca aaaaaatagc tgcagctatt atagaaagca acgctctatt tttagctaat
     3241 ccttttaaag atttcaacaa tgaaactttt aactgcgatt catcactttt aacccgttct
     3301 acagttaatt tataacatat tacgtaacaa ataactgcac acactgaaaa tactcctgcc
     3361 acaattgtaa aagttgatcc accacgtaca atttgagctc cagaactatc tcttgtataa
     3421 ataaaaattg gtgttaacac gccaatgaac atacctgcta aggtagctcc tatactacga
     3481 aatgttgata acgctgtcct ttcttctgca acagaagtga tagctgatgc cattgaaccg
     3541 taaggaatat taatagatgt ataaaaaata cttccccata gtatatatgt tacatacata
     3601 tatataactt taatactcat agaagctgat gccattgatg tttgatacat taaaaaactt
     3661 gctaaagcga ctggaccact catccgtaaa atccacggtt taaatttgcc agttttagat
     3721 ggttttgatc catccacaat acgccccatt gttatgtctg taactgcatc aataaatctt
     3781 gctactaaaa ataatgttcc tacggtagct ccgcttatcc ccaatacttt tgtataaaaa
     3841 atcattaaaa aagaactagc aaaaatgaaa gtgaaatcat tcccaaaatc tccaaacata
     3901 tatcctattt tgtctcttag accaaatttt ctttccatat aatttgcgct ccttcctatt
     3961 gttaagcgtt ttcattctgt tgaactgcca acttacgtac tatactctga agtataaaga
     4021 gttagtattt attttttaca ttttatagta ttatttattt tttctcctaa taattcttta
     4081 tctcataaaa cttatctata tatttactag ctattcgagt tacttcttca aaatcacctt
     4141 tttcaacagg agccaacaaa ttcccaccta caccaactgc aacaacgcct gcttcaaacc
     4201 actcttctaa gttatctaga cttactccgc cagtaggcat tatatttaca tatggtaatg
     4261 gagcttgata cgtttttaca acagatggac caaaagcaga acctggaaat agtttaatga
     4321 tttctactcc agcggtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat
     4381 aaggaatttg atataaatta caaatctctg ctgtttgtcg atcaaaagat ggacttacga
     4441 tatattctgc tccagccata attgcaatac gagcagtaaa cgcatctaaa actgtaccgg
     4501 ctccaattat tacatctttt tgatcagcat attttttagc aatttctttg attatttctt
     4561 cagcattcgg aactgtaaaa gttagttcaa tccctttaat tccacccgca gtaattgcat
     4621 ttattgcttt aatggcctca tctttatttt ttccacgtac aaccgcaatg actccagccg
     4681 cttctaactt acttaaaatt tctacttttt tcatatctat ctccttcttt tatggataca
     4741 tactctagct agttgtacta acttacaata aataaattta agcttttatt ttttgtatta
     4801 ttttctactt tcgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt
     4861 ctccactatc tgactaagat acatttcatc atctggaatt cgacctgctt taacccactc
     4921 gcttagtaat tgacataata ctcgacggaa atactcatgg cgaggatagc ttagaaaact
     4981 tcgagaatca gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgcg ccatcgtagt
     5041 caactgttga cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat
     5101 tttttgaatc ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcggt
     5161 tggatttaat gaatataaaa ttgtttttgg taagcaccca ctcttttcag cagtgtcgaa
     5221 caatgctaca acattttgtg tcaaactagc ttgatccccc atcgaatcac caccgacatc
     5281 tactccaatt tgttccttta atatacttga attatttctt aaagcattta aatgcatttg
     5341 catcgtccaa ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa
     5401 ttgtgcaatt tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt
     5461 gtcgagtatt ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa
     5521 tcgacagcca atttgatgaa aataatccac tcgttggctc aatgcctgct tcaacccatt
     5581 aaagtttttg attggtatac ccgctgcttg actaagtttt aaaatatagt tcgaaaatcc
     5641 tgatgatttg atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt
     5701 atcctgttta gaaagctcag tatgatatcg caagtcatct gtaggatcat ccgttgtaca
     5761 aatggctttc acatccatca tcttcagcaa ttcttttggg cgataggaat ccgtttggat
     5821 tttttcgttg gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat
     5881 atcaaagtaa cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt
     5941 ctccactgtt tttacaaaag ctaaaaattt ttcataatca ttacctgcac cactgatcaa
     6001 ctcttcttct atcccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt
     6061 atcatttaac catacctgag tcaaattctt gtattgctta ttttctagca aacatgctgg
     6121 gtctaaatga caatgataat cgatgattgg ttggtgttct gcaaatttat gaaaaagtct
     6181 tttagctatt tcattctcta ataaaaagtt cttatctaaa aacatattta agcactcctt
     6241 tccttccctt agtttttaga agagaaaatt tttcttctaa atagactcca ttatataatt
     6301 atttactcgc ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag
     6361 ctcggtcata taacccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat
     6421 ctggacgaat atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat
     6481 ctagagaacc atattctgat ttatgcggcg cttcataaaa atcgagtcca ttatcactta
     6541 aatatttgat attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata
     6601 gaatttcata gacatcatta tctggtcgtt ctgcaatact tccagtacat aacgtaatac
     6661 cattagaagg agaatccact acattacaca accattctaa atcgtcacga tttttaacaa
     6721 tacgaggtaa tccgaaaata gaatatggtg gatcatctgg atgaactgcc attttaatcc
     6781 caacttcttc acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgcaatt
     6841 tcgcttcatc aacttcttta taggcttcta ataattgctt gatttgtgat aaccgatgag
     6901 gttcccaacc cggcaattca aaattattag tagaattttc tacttttcta ataatttcct
     6961 ctggatcagt aggaatattt tttgaaatat aggctaacgt agaagaccca tcatctaaat
     7021 gataatccaa atctgatttt acccaatcaa agactggcat aaaattataa cagataacct
     7081 caatcccaaa ttctgccaaa ttgcgaatcg tctgtttata attctcgatg tattgatctc
     7141 gtgttggcaa accaattttg atatcatcat gaacattaac actctcaata actttcaagg
     7201 taaggccatt ggcttcgact tcatctttta aagctttaat tcgttctctc ggccagacct
     7261 ctccggctgg tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa
     7321 tttcttgtag tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag
     7381 ttactttcct ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc
     7441 tactagcatt tcgcaaaaga ttttttcaat tttctttcct atctcaactt gatataaatc
     7501 taaaccaaat atttccttat tttctaagat ttcctgtacc gcacaatgaa tctttgcagg
     7561 atcttgttca cctaatttca atactgagaa tttttcctgc aactctacca ataaaggatc
     7621 aggacttggc acaaaatttt ttccttgatc atcgactgcc aataaataac gtaaccaagc
     7681 agcaatagcg ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt
     7741 tttaattgtt tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg
     7801 cggtgtatct ggaatatagg gattaggcaa acgtttattg attacttcat caataaattg
     7861 ttttggatca ataacgcctg gactttcaac tactggtaag ccttctacat aacctatccc
     7921 tttaattaag ccaaccaaat catcatcttg catttctgcc tgaattgatt tataccccaa
     7981 caaacaacct gttattgcta atgccgtatg gagcggattt aaacatgtcg taaccttcat
     8041 gatatctgct ttatcaactg tttctctatc tgtcataatc acaccagccg cttccagttt
     8101 aggtcgacca tttggaaaac tatcttcaat gactagataa tggacttttt cagtattggt
     8161 aaaagcagcg atattggtat ttttcgcagt atgaattata tctaagtctt cgaaacccgc
     8221 cttcaataaa tccattttga cactttccga aggatttggc gtaattctat caatcatact
     8281 ccaaggaaaa gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacgta
     8341 tccttttttt tgccaagctt ctgcaatcat aaaaatgcta tcttgaaaac gttgaccatt
     8401 ctgtgagaaa ttatcagtag aaaccatcgc aatcggtaac tctccagcat taaacctgct
     8461 atacaacaag gatgtcacaa tactcatcgt atgttgagga ttggtaggtc cagattttat
     8521 atctgcttcc actaccgaaa aaaattgtcc tttagaatct tttaaactgt atcctttttc
     8581 agtaatagta aacgttgtaa actgtaatgt aggatcttca aaatatttaa ccactctttg
     8641 atagtccgtt atattctttg gattgcaata taaactatca gctgttactg ctaaaagtct
     8701 ttttttgagt tcaccatctt cacccataac aatctccaaa atatcattgt tgtagtcatg
     8761 ataagctttt tctactactt cttcatcaaa tgtctcacac acaactactc cggattctaa
     8821 ttcattttga tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc
     8881 tccaccaaaa tgaagccaca ctggatgttt ctgaccttct tgtttgaccg cttcgacatt
     8941 aaaatacggt agttgaactt gcattttgtt caatccttcg atagaatctt gccagtcttt
     9001 aatcaaaacc atatttaccc ctccatactg atatgttagt ttccgttaca attgtagtat
     9061 aaaatcgaat gtaatcgctg tcaataagaa aaaactaaaa atattctttt ttctaccatg
     9121 tcagaattat tgtgtctttt tttagtaaaa gcacaccaat atatattttt tagaactaac
     9181 ttaaattaaa gcttaatttc tctttgatct tatttgagta ttttttgttt ttttaatagc
     9241 tagtatgtta ctttgaaatt tgatattata taacgagcat agaactaaaa tatcaaattc
     9301 aaaaatgaaa ttttgagagg gattaaatat gacaattaaa actagtctcc aaaatcaagc
     9361 ttatcaatct attcgtcagc aaattatcta cgctgactta gaacctggaa aaaaagtatc
     9421 agaaaaaaaa ttggaagaaa cgctttctat cggtcgtact ccaatacgtg aagcattgat
     9481 tcaactccgt catcaaggac ttgttgatac aattcctcaa agtggaaatt atatctcatt
     9541 aatcgactta gatttagcaa aaaatgctcg ttttgtcaga gaacatttag aacgacaaat
     9601 tatgctagag tgttgtgcca agatgaataa tcaaaagaaa caagttttag aaactataat
     9661 tgcagaacaa gaaaaagctg ttagtcaaaa gaatgctcgt agtttttttc ataatgataa
     9721 tttatttcat aaagcttgtt ttgaaattgc agaacgggaa gaagtatggc aatggttgga
     9781 agataataat acgcacttgg aacgatttcg ttggttacga gtaaaaacgg aaggattaaa
     9841 atgggaaaaa attatggaac aacatcatca actatttaaa gccttgacca tgcaagaccc
     9901 tgaagaagct aacttcttaa catccttgca tcttcattta atgcttgatg aacaaaatgt
     9961 tgtattgact actcacccaa aatattttaa aaatatataa ctgctaaatt tctttttttt
    10021 aatttattta catttctgat tactacagaa aactaactac tttttttatg taaagttctg
    10081 tcaaatatag tcactttata ttactttctc ttgagtaaaa aaacatgtga ggtcgtaaac
    10141 aaagattgaa ctatctacat tcatactcta taatctgttt atttgcagac tgataatagc
    10201 aaaagaaaac ttataatcaa ataatatcaa catttgatca cataatttga gattgaatgc
    10261 tgatttacct atgttttacg taatggtctt tagttatttt tgctcataaa atgcataaaa
    10321 cacgcgtttt tatgtgtgct tgaaatttct tggctctata atttctaaga ctgataaaaa
    10381 tattctatcg gttctttttg caaataaaaa aacatataga agctccatta gattgaatca
    10441 ccacaacaca atcaaaggag cattccgtat gcttgatttt cattctataa aactactttt
    10501 aggaatcaaa gaaaatcaca acggttcaaa ctaaaagcaa gaatatccga agtaccattg
    10561 tttcatcgat catgagaaaa gcattcacct ctgtctgcag atgttcttat atcgctctgt
    10621 cagtcctatt aataaagtac tgttttgttt tcaaaaaaac tattacgagt actttttaag
    10681 agaatgcctt gcaaatattc atcacacacc gttatataag tcaaatagtt tctttcagtc
    10741 gtcaagaaaa gattctcctg tccttggcat tatcagttaa gtagcaactt aattctcgga
    10801 cttgttctac tctagcaaaa tttattttca tcaacttacg tccaaatgtt tttttgaata
    10861 attacttcat cgcgggtctg gaagacttgc ctcattcgat aagtcactag cttttggcgt
    10921 ccttcaggat ttctagtata caccaactga catttgggca gcgacgattg atttaattcg
    10981 tttagaaaaa ggttagcgat ttgcttattt cggccaaagc gttctggtac cgtcagaact
    11041 atctttttcc cttcgattaa ataccgcgga ttctccagcg gtgctaaaag ctccctcata
    11101 gcttcagtaa aaagctgtcc ttctcgatag ccagcattct caagatagca tctaaattcc
    11161 tgctgatcag ccgctacgat tacttttcta tcactaactt caccaaggcg ctgcaacgtc
    11221 gtttgaacgc atatggccaa gctttttttg cgggcacatt ccttttccca tagcttttgt
    11281 cgcctacgaa tttggaaata ttccagcaag ttacctttaa atatagctga ccagaagctt
    11341 agaggctcta tattttttac tactatcggc aatgaagttt cttgtttttg ctttacctga
    11401 tcgactttcc taacgacgat tggcatagat aggtgccagc catcagctaa agcagtactc
    11461 cattgttctt tcaaccgact tcgttcacaa gaaaaataga acatcgactg agtataagtt
    11521 tccatcgtgg tggtcgtata ttccagtggt aaagcaaagc gatcgccacc actctcaatt
    11581 tttggtgaag attcaaaggt cagacctgaa atattgccca aacgccgtag cattcttttc
    11641 aaatcaggct gttcagacag aggaactccc ggtaatccaa tcgctaaatg ccaaatgttc
    11701 gttactttat tttgtgcacc taggcgtaag ccccgccctc gtagttgttg tgtttgcaca
    11761 aaagtggaag cttgattagc caagatcaac agattaattg caggacaatc ccagccttct
    11821 cctagcaaac taacggttcc taccaaaata ttcaacttct tttcggttaa taggcgagta
    11881 actgcttgca tcaatgcttg gcggtttttg tcatccaccg tcaaataaaa ataacccgac
    11941 atataaggat gttcattcat acccgtcgct agttctgcca gctcaacagt tactagcatt
    12001 ttcggtaaca gtaagaactc tccacagatg gcaccgattt gctgagtgtt acctaataga
    12061 cgaacaactt catcaaacaa aggaaccacg ccataagcta aggtaccatc actgcctgct
    12121 aatacctcct tgcaaatacg atccatcaaa atgaccgctg tcagttcttg cttcagctga
    12181 acttcttctt ttaacaaaat ttgacaaact gcttgctttt tctcaggaat cgcctgacta
    12241 ccaacagtgt ctggatactc tggaaacaaa tgcagcgact catcaaacaa tcatccagct
    12301 tttttcaatt gataaaaaat aggcaattcc ggttgatctt caaacatctg ctgataaata
    12361 gtgatcagct cttttttacc taaagatgga actgaagctt ttttaccttg ctgcttagaa
    12421 ctgcctaata gctgccaatg ttcgggtatc aattcatagt tatgatgatt caagtaaatt
    12481 aatgcactag tgaacagctc aaaattttca taaatttcct tcgaatgctc aagcggatgc
    12541 tgaacaaatt ctaaagaaag caaataatcc accgtctctt ggctttccat taacgcttcc
    12601 accagctcat tttgtttctc taaaaaggta aaataatgct gagtagtttc ctgattagcc
    12661 ggaatcaaat atagaaaatc ctgatgagga catagaacct cctcctttac caactcggga
    12721 atgctaattt cttcgtcaat caggccattt taactcaata tattgcttcc actccgtctg
    12781 agagctatca atcggaggcg tggctgtcaa ggaaatcgtc aacagattcg gttgtttttt
    12841 cttcaagtca atcaacaatt caccccagct tttttttaaa tgatgagctt catcaaaaaa
    12901 ctaacagctc cagctgatca ccataagttg gctcctcctt cagtcggttg taaatggtct
    12961 gatacgtttc cactgtaata tgactaggat gttcaatcgc cccagaaaga tagcaactat
    13021 ctgctggatt tactaagaaa tattcctcga ttgttgttaa ccactgtttc ttcaatatta
    13081 gggaaggaac caaaattaag gttttacaat ccaaacgtcg gatcatctca attcctaaaa
    13141 aggtcttgcc agcccctggt ggagccacta gatggactga attgttattt aaataagtag
    13201 caaagttagt caatatacgc ttctgatagc tacgccactg aattttttca tcagatagcc
    13261 acatcccagg tccccctctc acgtattttc tctatcataa tctaaatgaa tacagcaaaa
    13321 aaaagatttc ttcaagaata tgtgttttta tagtaatatt ttacttactc taaactcaaa
    13381 tgaaaaggta aaaaaactgg tagtacaatc ttttaaattc tcattgtttt gtacgttctt
    13441 tactatcaaa aaaacaaaaa aaacgcgttt ctacgcgtga
//
LOCUS       DARBEG010000072        13429 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000072 DARBEG010000000
VERSION     DARBEG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.073"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SCK20_002109"
     CDS             complement(<1..914)
                     /locus_tag="SCK20_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG1146146.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SCK20_002110"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SCK20_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG1146147.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="SCK20_002111"
     CDS             2006..2176
                     /locus_tag="SCK20_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146148.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="SCK20_002112"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="SCK20_002112"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG1146149.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="SCK20_002113"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="SCK20_002113"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG1146150.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="SCK20_002114"
     CDS             4440..5321
                     /locus_tag="SCK20_002114"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG1146151.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="SCK20_002115"
     CDS             5318..6034
                     /locus_tag="SCK20_002115"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG1146152.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="SCK20_002116"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="SCK20_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG1146153.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="SCK20_002117"
     CDS             6751..7752
                     /locus_tag="SCK20_002117"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG1146154.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="SCK20_002118"
     CDS             7749..9110
                     /locus_tag="SCK20_002118"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG1146155.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="SCK20_002119"
     CDS             9070..10221
                     /locus_tag="SCK20_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG1146156.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="SCK20_002120"
     CDS             10196..11365
                     /locus_tag="SCK20_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1146157.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="SCK20_002121"
     CDS             11540..12919
                     /locus_tag="SCK20_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1146158.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="SCK20_002122"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="SCK20_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DARBEG010000073        13404 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000073 DARBEG010000000
VERSION     DARBEG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.074"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            79..1125
                     /locus_tag="SCK20_002123"
     CDS             79..1125
                     /locus_tag="SCK20_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1146159.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="SCK20_002124"
     CDS             1474..2106
                     /locus_tag="SCK20_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG1146160.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="SCK20_002125"
     CDS             2694..4133
                     /locus_tag="SCK20_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1146161.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="SCK20_002126"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="SCK20_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="SCK20_002127"
     CDS             4895..5110
                     /locus_tag="SCK20_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146162.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="SCK20_002128"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="SCK20_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5770..6681)
                     /locus_tag="SCK20_002129"
     CDS             complement(5770..6681)
                     /locus_tag="SCK20_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1146163.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="SCK20_002130"
     CDS             complement(6700..7704)
                     /locus_tag="SCK20_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1146164.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="SCK20_002131"
     CDS             complement(7701..9824)
                     /locus_tag="SCK20_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1146165.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="SCK20_002132"
     CDS             complement(9829..12276)
                     /locus_tag="SCK20_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1146166.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="SCK20_002133"
     CDS             complement(12263..12652)
                     /locus_tag="SCK20_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1146167.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="SCK20_002134"
     CDS             complement(12712..13215)
                     /locus_tag="SCK20_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1146168.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..13389)
                     /locus_tag="SCK20_002135"
     CDS             complement(13228..13389)
                     /locus_tag="SCK20_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146169.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4099 a         2642 c         2454 g         4209 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DARBEG010000074        13399 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000074 DARBEG010000000
VERSION     DARBEG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.075"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(15..707)
                     /locus_tag="SCK20_002136"
     CDS             complement(15..707)
                     /locus_tag="SCK20_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1146170.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="SCK20_002137"
     CDS             complement(1144..1713)
                     /locus_tag="SCK20_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1146171.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="SCK20_002138"
     CDS             complement(1787..2110)
                     /locus_tag="SCK20_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146172.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="SCK20_002139"
     CDS             complement(2264..3076)
                     /locus_tag="SCK20_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146173.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="SCK20_002140"
     CDS             3430..4278
                     /locus_tag="SCK20_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG1146174.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="SCK20_002141"
     CDS             4423..5364
                     /locus_tag="SCK20_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG1146175.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="SCK20_002142"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="SCK20_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="SCK20_002143"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="SCK20_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG1146176.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="SCK20_002144"
     CDS             complement(9828..12014)
                     /locus_tag="SCK20_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG1146177.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="SCK20_002145"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="SCK20_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG1146178.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="SCK20_002146"
     CDS             complement(12236..12676)
                     /locus_tag="SCK20_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG1146179.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="SCK20_002147"
     CDS             complement(12750..13289)
                     /locus_tag="SCK20_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1146180.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DARBEG010000075        13269 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000075 DARBEG010000000
VERSION     DARBEG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.076"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="SCK20_002148"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="SCK20_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1146181.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="SCK20_002149"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="SCK20_002149"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG1146182.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="SCK20_002150"
     CDS             3349..3552
                     /locus_tag="SCK20_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1146183.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4315)
                     /locus_tag="SCK20_002151"
     CDS             complement(3653..4315)
                     /locus_tag="SCK20_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1146184.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4328..5830)
                     /locus_tag="SCK20_002152"
     CDS             complement(4328..5830)
                     /locus_tag="SCK20_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1146185.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6378..8057
                     /locus_tag="SCK20_002153"
     CDS             6378..8057
                     /locus_tag="SCK20_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1146186.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8184..8663
                     /locus_tag="SCK20_002154"
     CDS             8184..8663
                     /locus_tag="SCK20_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG1146187.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8882..9562
                     /locus_tag="SCK20_002155"
     CDS             8882..9562
                     /locus_tag="SCK20_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG1146188.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9570..10901
                     /locus_tag="SCK20_002156"
     CDS             9570..10901
                     /locus_tag="SCK20_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1146189.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10916..11878
                     /locus_tag="SCK20_002157"
     CDS             10916..11878
                     /locus_tag="SCK20_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG1146190.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11989..12327
                     /locus_tag="SCK20_002158"
     CDS             11989..12327
                     /locus_tag="SCK20_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG1146191.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12406..13113
                     /locus_tag="SCK20_002159"
     CDS             12406..13113
                     /locus_tag="SCK20_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG1146192.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4230 a         2338 c         2907 g         3794 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3961 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     4021 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     4081 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4141 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4201 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4261 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4321 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4381 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4441 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4501 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4561 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4621 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4681 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4741 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4801 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4861 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4921 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4981 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     5041 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     5101 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5161 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5221 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5281 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5341 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5401 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5461 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5521 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5581 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5641 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5701 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5761 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5821 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5881 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5941 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     6001 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     6061 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6121 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6181 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6241 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6301 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6361 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6421 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6481 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6541 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6601 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6661 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6721 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6781 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6841 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6901 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6961 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     7021 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     7081 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7141 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7201 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7261 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7321 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7381 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7441 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7501 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7561 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7621 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7681 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7741 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7801 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7861 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7921 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7981 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     8041 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     8101 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8161 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8221 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8281 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8341 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8401 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8461 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8521 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8581 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8641 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8701 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8761 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8821 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8881 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8941 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     9001 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     9061 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9121 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9181 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9241 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9301 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9361 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9421 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9481 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9541 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9601 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9661 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9721 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9781 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9841 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9901 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9961 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
    10021 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
    10081 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10141 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10201 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10261 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10321 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10381 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10441 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10501 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10561 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10621 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10681 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10741 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10801 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10861 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10921 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10981 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    11041 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    11101 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11161 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11221 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11281 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11341 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11401 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11461 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11521 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11581 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11641 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11701 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11761 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11821 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11881 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11941 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    12001 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    12061 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12121 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12181 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12241 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12301 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12361 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12421 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12481 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12541 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12601 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12661 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12721 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12781 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12841 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12901 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12961 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    13021 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    13081 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13141 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagggctc
    13201 tttgtcaata aggactgatg ggttgtacaa aatcaaatct gggtcagaat gaacctcatt
    13261 ctggctcag
//
LOCUS       DARBEG010000076        13128 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000076 DARBEG010000000
VERSION     DARBEG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.077"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            260..466
                     /locus_tag="SCK20_002160"
     CDS             260..466
                     /locus_tag="SCK20_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG1146193.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(668..1669)
                     /locus_tag="SCK20_002161"
     CDS             complement(668..1669)
                     /locus_tag="SCK20_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1146194.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1674..3587)
                     /locus_tag="SCK20_002162"
     CDS             complement(1674..3587)
                     /locus_tag="SCK20_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1146195.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3755..4261)
                     /locus_tag="SCK20_002163"
     CDS             complement(3755..4261)
                     /locus_tag="SCK20_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1146196.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4421..4861
                     /locus_tag="SCK20_002164"
     CDS             4421..4861
                     /locus_tag="SCK20_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1146197.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5020..6360
                     /locus_tag="SCK20_002165"
     CDS             5020..6360
                     /locus_tag="SCK20_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1146198.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6429..7586)
                     /locus_tag="SCK20_002166"
     CDS             complement(6429..7586)
                     /locus_tag="SCK20_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146199.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7589..7945)
                     /locus_tag="SCK20_002167"
     CDS             complement(7589..7945)
                     /locus_tag="SCK20_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146200.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8243..9217)
                     /gene="sgrA"
                     /locus_tag="SCK20_002168"
     CDS             complement(8243..9217)
                     /gene="sgrA"
                     /locus_tag="SCK20_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG1146201.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9413..10252)
                     /locus_tag="SCK20_002169"
     CDS             complement(9413..10252)
                     /locus_tag="SCK20_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1146202.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10437..11324
                     /locus_tag="SCK20_002170"
     CDS             10437..11324
                     /locus_tag="SCK20_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1146203.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(11908..12603)
                     /locus_tag="SCK20_002171"
     CDS             complement(11908..12603)
                     /locus_tag="SCK20_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG1146204.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12587..12985)
                     /locus_tag="SCK20_002172"
     CDS             complement(12587..12985)
                     /locus_tag="SCK20_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1146205.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3930 a         2652 c         2034 g         4512 t
ORIGIN      
        1 atacatcgaa tttttgggga aattcttttg ggggtatcct aaatcttttc tattatattc
       61 ccatattttc tattattggt ctagacgtat gatggtaaac tttgtaccta tctgattgct
      121 gaatcattaa ttgcttatca ttagccatct ttgtttcaag ttcgctagat gatcatttta
      181 ttcacatcct aacaaacttg tcacatttat agattcttaa tctatttatc atacaattat
      241 aaaaaaggag gtgctgctta tgaaagctat ccatatttct ttgctttttt tcgctagtgc
      301 attgatttta tttgctattc atctttgttc ccagccttat atcaataaca acggaatatt
      361 agtggaacca gcattctttt gcttaccact cggctttttg tcccttcttg cctccgcagg
      421 cttcggattt gttgcttttt ttaaacgatc aaaacaacaa atctgactta taaaatttac
      481 gtaaaagaga tcgtgacaat aatcgtgtga tattcccaaa tttcgaataa aaggtgcccc
      541 ttgccatctc taactcctca gtcacaagtt acaatattca taaaccatga aaagcttgtt
      601 tattgaaatt gctccttgtg acttggagct aagtgacaga ttacaacctc ttttgcatca
      661 taagacttta ctccttgaaa aacattacta acttttgata tgtttcttgc gcttgtttat
      721 tgctgaaatc aaactgatat tcatggttca aaacagcatt tttctccgca taatctactt
      781 ttgtgacttt cacccccgct ttctcaaata cagaagttac ttcttccatt tgtttcgtaa
      841 acgtatttgt atttccatcc gttaagaatg tgttttgcgt ccaacgatcc gcatgtctga
      901 ccaaggctaa acttttgacc ttctctgatt gttcaaagtc cttattatca aagtaagccc
      961 atccacattt ttcataaaga aagctagaga ttttatcact tacttgagta agtgcagaag
     1021 gatccaacag accagacaaa gaaataaact tcgtaatctg cttgttctcc aaagtcggat
     1081 caagatgatc agctttttta atttcttcat tttgaagtgc taacaaatat tctccactaa
     1141 tttgtgcacc tgccgaattt ccaccaatcg aaatattcga ccaatctgct tgatctttta
     1201 tctctttttt agctgcttga atggcttcgt tcaattggat aactggtgta ggatgttttg
     1261 cttccggtga tcgttcataa tctatattta cgatgatttg ctgcgtgtct gaacatagat
     1321 attccaaata atcacgacaa tcatttttat cccctcctac atatgcgcct ccatgcatcc
     1381 agaaaagaat cggtaatttt ttcttttcca ctttcggtgt gtagatatcc atattattat
     1441 ttttgtactt tgaaggatag gatatttctt tttttatatc gatttgttcg attttttgct
     1501 ggaaatcatc tggtcgggca tatacttgtt ctttttcact tgtgtcaaat tgttttctaa
     1561 caagcaagct aatcggcttt ggtgtgattt gattaatcaa aaatacagtg actaaaataa
     1621 ctacgatgat aactggtact gcaatcacta ttcgcttgaa ccatttcatt catttaccct
     1681 ctctttcttc gccaaatcca tgtgataatc gtgctaacag ataaaaatcc acctgaataa
     1741 atgagaagcg aatgtttctt ttctccagtt ttcggcaaca ttcttttttt tgcatctgtc
     1801 tttttttgta ctttcactgt catctgaatc ggttgcgtat catcacttga ttgatcctct
     1861 cgaggttcat tctttccctt attgtctgca ttctctgtcg gatcttgctt ttttggtgga
     1921 gctactggga tttttttgta atagaaaata atagttggta tcgtctcatt aaaaatccct
     1981 tcagtcggtc cttcaacatt tgctaattcg tatccttcga atgtcttttg ctctgtcata
     2041 taattttctc cataagctcc gtaaagctgg atttcgtcct ggtctttgag tagactttta
     2101 ttgttttcat ccacatactt tacgtttatc tgatcatgga acactacttt caaaaagtcc
     2161 gttacatgtt tcgtgtaggc agcatggtca acttcctgtg cctttccatg ccctgctcct
     2221 tctactaata aaagatcttt ataccctcct gataactcat ataatttttg cgcgttagac
     2281 acaggtacta catcatctgc tgttccatgg ataatcaatt taggcaatga atctgacatt
     2341 ccaatcgtag gtagagcagc ttttgtatcc aaatgcatct cctgatcaaa atagtattgt
     2401 ttcaaaaggc ccataaataa atcttcatgc tcttgatccc caatgatgtc taactgattt
     2461 tttatggcat ccggtagtgc gggggctact gtctggctgt acgtaaaacg tagttgttca
     2521 tcaattgatg caaatccgca attttcaata attcctttga cattgacagg aatatctttc
     2581 gctaatacag acatcatcgt agctgctccc atcgaaccac cataaagtaa aatcttttga
     2641 gatggccgct ctcgaacttc ttgatttatc cagtaaagga cgtcatctga ctcatattgt
     2701 ccataggtca cataatcgcc tccgctatta ccagtcgctc tggaatcaac aaataataaa
     2761 ttatacccgg ccttataaaa atcattgtat tcttctgtaa cgatcccgtt attccaattc
     2821 cctcgaaatc cgccatggat catgactgtt ttatctgaac cttgatcgac ataatatgca
     2881 tatacatcca tcattttgcc atcttgtttg tttagtacag tttgatgaag ttgttttgta
     2941 tttggataga agcctgtgtt ttctgtaggc gttgtcccat catctgtacc tccaagcatc
     3001 gacatcaaca cactgttttt tgcaaaagcg atatcgtaca atagataagt agcaatcaga
     3061 tgtttgataa tctctttatc tacaccataa tcattaacag caagctcagt gactaataac
     3121 tctattaatt ttgacttgat gtcttctgtt ggtccattaa gtatttctgt catctgttta
     3181 ccgaagtaat tcaaatattc tttaccattg aaattttcac ctttaccgat atcatcattt
     3241 tctgtcccat tctctttgtc acttacttta gcagaagagg attcgtcgtt tgacattttt
     3301 gtagctgttg cttcttctgt ttgttggtta tctgtggtag cggtcgtttc ttgatctgtg
     3361 ctagtaacag atgtctcaac cgttgtttca gtatttgttt ctgtttgtga agtactcgat
     3421 ttcgtttcat ttccagcatc agtagtttct gtggtttcta cctcccgaga ttccgattcg
     3481 gaagaaatgc tctctccata tactgtccat tgcgggacgc ttgtacttac agcaaataag
     3541 atgaccaacg ctccctttaa tacttgtttt gttctcaagc tattcactcc tttttatgtt
     3601 tctaggagaa ttatatgtaa acgcaaacat tttttataca aaaaataaat ggtttatccc
     3661 ataaaaatag aacaggatta ttttctcatt ttacgtaata aagcagaatg gataaaccag
     3721 tatccattct gcttttttta aacgaagaaa caacttattg ctccaaaaat gtcagaaatc
     3781 gtttatccca aattttctcg taaaaagcga ttgtttcctt tgctttcata aaaggattat
     3841 cataagttgt cgttgctcct ctacacatca taagctggta tcctaatcca tgagcaaact
     3901 tcacagtcgt atctatacaa tactctgctt gtgccccaca taattcgatc gaatgacagt
     3961 ccaattcagt taataaaaca ctcaaattag tcttataaaa tgaattcgca tgggtttttc
     4021 tcacaaaata actgtgctca ggaatagata attccgacaa tagctcccat ggttttgttt
     4081 gtggcactaa ttgagcatct tcgtgttgaa cgaaaataac tggtttccca atcgtttcgt
     4141 atgttttgat tcttttattc actaatgctg tcactgtttc taattcgtgt atcggcgaag
     4201 aatcaaaaca tacccctttt tgtaaatcga tcactactat tgcgtcggct gtgtttgaca
     4261 tacttgtgcc ttctttcttt tttcttgatt taatagtatt tcttatctag ctagttccat
     4321 tatacgtgac aacattttct tattttacac ctctaaatgg cacttttcga ctatttcttc
     4381 atgttattat ttcataaata cttagctatt ggaggaatgt atgatgatta aattgtatac
     4441 aggttctggc tgctcgtctt caagacgcgc aaaacgttgg cttatcgtcc atgatatacc
     4501 gtttatcgaa cagaacttaa ttcgtcaacc actaaccaaa aatgaattta tccacttact
     4561 aaaattgaca aacaacggcg tgaccgaaat gctctctact cattctatta gttataaaaa
     4621 tcttgttcac agaatagatg aactcagttt gagtgaacta tatattgaga ttcgtacatc
     4681 cccttcttta ttgaaaaaac cgattatcta cgaccaatct gcaaaacttt tgactggatt
     4741 tagcgaagat aaaatcagga tgttcctttc taaaactaaa agaaagcaag agctcaacga
     4801 actcttgctt tctggtcatc ttgtcgattt taaagaaaat aatctttttg caatgtctta
     4861 attttggcat aaacaatctg cttttctgga tctataatat ctagtgttgg tgacgccttc
     4921 tgttgccaat actttttctt ttttctaata aacgcaaaca gacaccttga aatcctttag
     4981 aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga tgtcctattc agagtttacc
     5041 aaagagttac tagatattct agacctaaat cttacctttc atgaagatgc ctttcgcaaa
     5101 gagcgcatta atgacgaaac atgttttgtt tttgatggca cattgaccta tcaaccagag
     5161 gaatgcttcc attgccacta tcaaaataag caaacgatca ttaaatgggg ctggaaaaaa
     5221 gtatccatct tattgaatga tgtcagtaac tacaagacca ttgtgcggat taacaaacag
     5281 cgcttcaaat gtaaacactg tggaaagact tttttagcag aagattcagt tagcgatcgt
     5341 cgctgctcca ttgcccgcag agtcaaacaa gccattcttg agctattaag tgagcctatt
     5401 tctatgtctt tgattgcacg tatgaagcac atttctccga ccactgtcat tcgaatactt
     5461 cgcagccttc ggcctaaaac cgtttctttg aaccagccgt taccagaagt ggtgtgtttc
     5521 gatgaattca aatcggtgaa aaacgtctcg ggagccatga gctttgtcat gatggacggc
     5581 aagacccatc aactgattga tattgtggaa aatcggcaac tcaatccttt acgcgactat
     5641 ttcttgcgct accctagaaa agtccgtgaa caagtacgtc tagtggtttc agatttctat
     5701 acaccctatc gcacattggt gaaagagtgt tttccgaatg cccgaatcgt cgccgatcgc
     5761 tttcatatta gccaacacat cggtcgggcc tttaccaacc accgaattca agtaatgaag
     5821 acctttaaaa aaggggatcg tcggtcgaag cacttaaaga aatactggag actacttcaa
     5881 aagaatgcgt gggaattgaa tggtcagcat cgctactggc gtccgtcatt tcgagatcat
     5941 ctgacagaag cggagatagt cgatcggcta ttatattatg atgactcctt aaagcgaggc
     6001 tacgaagtct accaggtttt tctttccgct atcagaagac aagatgttcc tgaattcgtc
     6061 gcgctcctaa aagaagacta caaggagttg ccagagcact atcaaccagt gttcacgacc
     6121 ttcaagaaat atcgaacaga aatcaaacga gcgttgcgag ttccctattc caacggccct
     6181 atagaatgtt tgaacaacca tatcaaagta ctaaaacgaa ttgcctatgg ctttagaaac
     6241 tttcaaaact accgagaacg aatcttttta tatcgaggaa aatatttcaa gaaaactaag
     6301 aacactaccc aactgactaa agccagaaca acaacacggc tagacaagat agctgtctag
     6361 ccgtgtgagt atctcgatta ttctattgaa tttcttcacc aacactcatt gacgaagagc
     6421 cgcttttctt aattaacgaa atccaatttt tccaatatgg atctattttt ctcattcctg
     6481 atttttatga tttcagcttt cagacgcgcc caatcatttt ttgtaggttg tccattccag
     6541 taaaatatct gtgtattcgt ttgatcatat gcctttttgt cgataggata atcgctaatg
     6601 acaatatcag gtaactgatc agcggatgca ctatatttta ttgggcgttc agtcatagat
     6661 aatatccttt tctttgccca taattcctgt gttttaccat actttgattg aatatagaat
     6721 atgacaggtt gttcgtgttt tataaaaata tccctgacta acatacagta ttgaaaaatc
     6781 aaccgtctgt tcttttcata ataagtatta agctctggaa ttctgcgagc taccccacta
     6841 aaaaactgtt gcatgattgg atacatcact ggaaatatat gctgaaaatc tctgtcttct
     6901 gctgattttc caaaagcatc caccttcttt tttgtcccaa aaacccttga atacaaatgg
     6961 atcattgcca aattgacata tagataattt ttttctgaag ttgtaaaaca aatcttgaag
     7021 tttttttcaa aattttctac aatagacgat tcaatccagc cccaactttc ttgtacttta
     7081 tctggagtat ccaacaaatc ttcttccaaa taggtgttca ttacaccaaa cataaaataa
     7141 aggtaagctt gctctgttgg agtgatgtga ttcatcgacc agaagtttgc ttcaaaaaaa
     7201 ggctctagta tgtaatgcgt aaactcttct tttgttatca atggatgcac aaatttgttc
     7261 atctcttctg ggacatacgt aatcgagtgg ccttgtttca ttcgttgcaa cgaaattctt
     7321 agtaggctga tccatttctt tctatctata ctacgcgtat atggcgcatg tttcgcaaat
     7381 ttttctaata gttgacgttt attttttata cttgataata tatcagaatg attttcgtcg
     7441 aatgactgcc aatacatcaa gtgaaaaaag tatcggatac ggtattcttc tccttctatc
     7501 ggattacctt ttttcaagct gcaagataaa tgatagtttt tcaaaatagt attaagttct
     7561 cttaattttc tttgaatcgt acgcaaactt atataatttt ttgactaaga ttttctaagg
     7621 attgataagt acctttgtaa agtgaatcca gtaaaatata accaatcgtt ccctctaaaa
     7681 gtatagaata aactttactc agtaaaaact gagaagaaaa ctcgactttt atcatcttgt
     7741 tttctgaaag aaaagataat ttatattctg gatacctttc gcaaaacgat tctgcttgtt
     7801 gaatcaccga attcatctta gccagggtgt agtttagatc taaagcgata tccttcacca
     7861 catataattt ctcgtctctt tcataacaat agttcaaaaa ttgatatact tgcctttcat
     7921 tgatatcaat aaattcttca aacaaagtac atactccttt ttatctatta tttagactta
     7981 ttccgacagc tacatgaata acgtacaaat agattcgtta ctgatttttc agagatacta
     8041 ccatgcgcag tgtgcattgt caattattct tgctcttatt ttccatttgt gttcctgtta
     8101 tttcctgttt ctcaaatatg ttagtaaaca aaaatagatc tggcaaagct acaacttcgc
     8161 caggcaatac agcactctcg aatgaaatat tcactcaaaa atctatcgtt ttattcaatt
     8221 ttagatctgt tgcttttatt ctttatttca ctcgatggaa gagagaacga aaatagcttg
     8281 ctcctacaat caaaatgagt aacacaccca tccatgtaat tagcttgttc aaggttctac
     8341 taccagtttc tggtaaatcg ctttttctat tatctgtttc tttcgctttt tcttgatgat
     8401 caattccacg agaaacaaaa tcaggctgtt ttagcacact ttttaggttg ctccctgcat
     8461 ttctcttttg ttccttagtt ggtatgatag gtaccaaatc aagttcatta tttacttttc
     8521 ctgtgctgct ttcgctgctc gtgcttcctg tgctactctc actactcatg cttcctgtgc
     8581 tactttcact actcgtactc tccgtactgc tttcgctgct cgtgcttcct gtgctacttt
     8641 cactactcgt actctccgta ctgctttcgc tgctcgtgtt ttctgtgcta ctttcactgc
     8701 tcgtactctc tgtactgctt tcaccgctcg tgttttctgt actgctttca ctacttgtgc
     8761 tatccgtact gcttggcggt ccaacaactg tccaactgct tggtttcaaa ttctccgtga
     8821 tcgtcatctg tccatcataa aaaacaatca aatcaacttt atctttactc ttgatctcat
     8881 caatcgatag ttctgcagaa atcattattg tatactcaat accagcggtc cattgtacgt
     8941 cttttaaata aacttcccca ataacttttt ctgaacttcc attaccatcc caaccatatg
     9001 ttagaacagc atgtacatca ctagcattca cgcttttgtt tggtttgact ggaatagtaa
     9061 tgacttgttg tgtcatgtca taaacaatcc gatcagtttt cacagtaaat gaaagattct
     9121 catttgcccg ttcatttgca gaagtactga ttccaatcat tgctgcaatt atactcagcc
     9181 caagaataac aaataatacg gtcgctgttt tcttcatttc tatcactctc catataatta
     9241 ccttctattt gataaaggaa ttatataaaa tgaaaatgat gagaacacga cagtcagttg
     9301 tgaaaatcta acaaaagatg tcgtgtattc gcttgaattt gtttactttt tcgtttaatc
     9361 aatgacagac ttaaaaaagc aagaccgata tcagccttgc tttcttcatc ttttattgat
     9421 aaacagcagt gtatttcttc atccgctttg ttttatatgc atagagtaac aaaccaacaa
     9481 tcaagactaa caagaataat gtagttgata tagatgcttc agccccaaag tttcccccag
     9541 taattattgt attttgcgat tggatcagag tggagaaaaa accttgttct tgcgttccgc
     9601 tgactggtat acccaaaata gctccttgtg tccaattcca agtaatatga gcaccaatag
     9661 ccgtaaataa acttcctgta ctgtagataa gtgcgctgaa aaagcaacct gctaaaaaga
     9721 tattgaaaac tgccagaaat gtcatgttag gattcaatcc gtgcaaaaga gcaaataatg
     9781 cagcagatcc aatcattgct attaacggac caaatctaag catctgatgc tgaaaaaagc
     9841 cacggaataa aagctcttct gatgccccca ctgcaataaa taaaataaaa ctcgttatca
     9901 gcggcacaat gtttgcgttt cctgaatcta accgatacgc ccccgtcaaa tataatggac
     9961 tagtaataag cagaaacact ccaattccaa caacgagacc tcccgctaaa tacccccagt
    10021 cttttcctcg aacagaaaca ccataatctt ctattctttt tccttcgacc ttactagcaa
    10081 gaaataaagc tagtaaaaaa acagctaaat gtccgaccgt accggcaatt gctccaatcc
    10141 attcactatt tccaataaac atatccgcaa gtgcattatt gacaagaatt cctaaaacaa
    10201 aaccaactaa gactattaaa acagatccaa tcaaccgaat aatcctgatc atctttcatc
    10261 cctcctttat attaagttta acaacccttt tctttcttgt ctcgtgaaaa ctatgtaaat
    10321 gttctccatc atagagagaa tagattttta cgaactaaga atatctacag caaccttacg
    10381 aaaatgaaag actgtctccc taattcttct gttagactgt ttttaataag gaaaggatgg
    10441 gaaatttttt gaaaaaacaa ctactaatta tgtgcctttc agcatgtgca attttactat
    10501 tctcaggttg tagcaagtcc aatgaaacga ttgcaacgtt taaaggtgga acacttacag
    10561 ttcaggattt ttatgataac ccaaaagtta aatcaaataa tcaaacacta ttaaaaaaaa
    10621 tgattgtctt caaagcattt gatgatgtat acggaaaaga gatttcttca aaggaagtaa
    10681 caaaagaata taatgaacaa atcaaaaaac tcggtccaaa ctacaaggaa caattaaaag
    10741 cagttggcca aacagaagag acatataaga aactctttaa acagatgctg gctttccaat
    10801 acggtttgaa agccaatgta aaattaacag ataaagatct agacactgct tggaaagaat
    10861 tctatccaga agttagtaca cagatcattc tattttcgac taaagaagaa gcagataaag
    10921 caaaaaaaga agcaaatgaa ggggaaaatt tctccaagct tgttcaagct tatggaaaaa
    10981 ataaaacatt aaaggaaact gatggaaaaa tgaattttga ttcaaccaat ccagagattc
    11041 ctactgaagt aaaaaaagca gcatttaaac taaaaaacgg ggaagtttct gatatcattc
    11101 ctgtaacaga tccaacgact tatcagcaaa gctattatct agtaaaaatg gtaaaaaaac
    11161 aggataaagg ttctaataaa gacaaataca aatctgagct agaaaaaatt gctaccgaag
    11221 caaaaactct tgatacggaa ttcatggata aaacaatccg taaagtgatg aaaaaagaca
    11281 atgtaactat taaagatcct tatgtaaaaa atatattcaa ataaacaaat ttgacccaat
    11341 tggggatgtg gcaaaatttg agaagtgatt tttggaaatt ccttcttatt tttgaggcaa
    11401 accacttttg ccacaacttc atcggggcct ttttttattt gtaccaaaac aaatcactgg
    11461 atttgttaca atcatgagga tataatttaa aaaagttttt tatttctatc acatgaatca
    11521 atgcgccatt aagtagattc tttcatgctt ccctattttt tcacttgccg tatcgcttca
    11581 aaaacaacta tcataaactc aaactcgact gaaaagttat tttaaaaaag actaaaaata
    11641 tcccttctgg acttgttcct cttattctgt tatactagag aaacacgaaa gatattttta
    11701 tgcgacactt ttcatttgtt tttatgaaat ttattcaaaa ctggcgtaac ttcagagtta
    11761 caaggatgta ttagaggtta gggaatacat cgtttaaatg agaaataagt aatagtcacc
    11821 caaactttaa attaatgaat attgaatcgt cagatagcct cctcgagttt tcgagaaggc
    11881 tatcgtttat tttatggaga aattgattca gaagtaatca gcgacctttt ttagttcacc
    11941 aatcgtttct gcttggaaag tttgcaccca atcttcttgg aagcccctca ctgctttatc
    12001 aatcgagggc atctctaata cttgatctag tagctcttta cctaacgtta tcgcatgagc
    12061 tccagatcga atggctgtag tgacttgttc acaatttttg aaactagctg ctaatatttt
    12121 tgttggtctt tgcgtacgtt cgatctcagt ccgcaacgaa tcaatcactt tcatagcatc
    12181 aatgccttga ttttgcatcc tgttataata aggtgcaata taatctgctc cattattaat
    12241 agctaaatac ccttgtaatt ctgtgtaaat agctgtaccg gtaatcaaat aaccttcttt
    12301 tttcaacttt ttgatcgctt gaaggcctgc ttgattcacc ggcaccttga taaacgtatc
    12361 ttttcccagt tcttctatga taactcgagc atcctctatc atttcttctt ccgttcttcc
    12421 tattgtctga atatggatag gaatctgtcc gattattttt ttgatttctt tcatatgcga
    12481 gaagaagttt gacgctcctt cttttttcag aattgtagga tttgttgtta cgcctgttaa
    12541 attcaaaaac tgttgacaag aataaatact ttttgtattt gctgtatcaa gcatgatttc
    12601 catatagctt tcccccattc tgatcctctt gatcgatttt aatgattgtc ccctttctta
    12661 aatgcggcat ttccttatct tctacataca ttgttcctgg aagttccggc tcatgagatc
    12721 catcaaattt tatcgtgata tgtcctaatt cattcaaatt agtttggaca acatctccta
    12781 ctgctgtgat tacatacatt tgtttatcaa aacagagctt catatcacaa gttatttctt
    12841 ttcttacctg attcaattca atcttataac aataatctgt taatgtttcc ggtgcttcat
    12901 ctccaaataa aatcattatt ttttctttcc tgaatacttc tgcttctata ccaacatctg
    12961 ttacaaccgt ttcaaagatc gtcatctctg ttcctcctcg taaaatatca tttgtctaat
    13021 agtttttatt caaaatgttt ctagattata aaatttattg aataacacat tcctaacgga
    13081 atttgtttcg atattgtgtg atttctcaga gtacaatgga tgtatgga
//
LOCUS       DARBEG010000077        12998 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000077 DARBEG010000000
VERSION     DARBEG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.078"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            64..765
                     /locus_tag="SCK20_002173"
     CDS             64..765
                     /locus_tag="SCK20_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG1146206.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            762..1883
                     /locus_tag="SCK20_002174"
     CDS             762..1883
                     /locus_tag="SCK20_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG1146207.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1898..3130
                     /gene="pepT"
                     /locus_tag="SCK20_002175"
     CDS             1898..3130
                     /gene="pepT"
                     /locus_tag="SCK20_002175"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG1146208.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3262..4170
                     /locus_tag="SCK20_002176"
     CDS             3262..4170
                     /locus_tag="SCK20_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG1146209.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4205..4477
                     /locus_tag="SCK20_002177"
     CDS             4205..4477
                     /locus_tag="SCK20_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG1146210.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4488..5534
                     /locus_tag="SCK20_002178"
     CDS             4488..5534
                     /locus_tag="SCK20_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG1146211.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5785..8394
                     /gene="clpB"
                     /locus_tag="SCK20_002179"
     CDS             5785..8394
                     /gene="clpB"
                     /locus_tag="SCK20_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG1146212.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8545..9231)
                     /locus_tag="SCK20_002180"
     CDS             complement(8545..9231)
                     /locus_tag="SCK20_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146213.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(9209..9703)
                     /locus_tag="SCK20_002181"
     CDS             complement(9209..9703)
                     /locus_tag="SCK20_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1146214.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9723..10943)
                     /locus_tag="SCK20_002182"
     CDS             complement(9723..10943)
                     /locus_tag="SCK20_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146215.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11090..12925
                     /gene="lepA"
                     /locus_tag="SCK20_002183"
     CDS             11090..12925
                     /gene="lepA"
                     /locus_tag="SCK20_002183"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG1146216.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         4257 a         2378 c         2727 g         3636 t
ORIGIN      
        1 ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag aaggagtgaa
       61 gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt tgtccctgaa
      121 tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc attgatatta
      181 agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta tgaatcagca
      241 cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct tgcaaatggt
      301 ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg tatgggaggt
      361 tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag cggacaagaa
      421 cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct gcaagaaaat
      481 cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata tgaaatcatt
      541 gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat gttcgggcca
      601 tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga gcttcaacaa
      661 agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg tcaaaaacaa
      721 atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc agcggacgca
      781 ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa ggagacccag
      841 tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg acattagacg
      901 ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg attgctaaac
      961 atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa gaaaaaatgt
     1021 atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac atggatatta
     1081 tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg gagagttatt
     1141 tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct gtggatcatg
     1201 cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt gattataccg
     1261 gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt gcacaaccag
     1321 cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag gttattttgc
     1381 cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct tatgaggaac
     1441 ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta ggacgagtag
     1501 gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa gaagcgttcc
     1561 aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg aagcgcattg
     1621 cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa agagcagatg
     1681 tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc gctgggttga
     1741 ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt atcgagaaat
     1801 ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct gaaacaaata
     1861 caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc ttttgccacg
     1921 ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa cgactccatc
     1981 gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag agttaggctt
     2041 agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac caagcaatgt
     2101 tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag attttaatgc
     2161 agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga tcgtactaga
     2221 tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa attatgaagg
     2281 ccacacattg atcacaacag atggcagtac attactggga gcagatgaca aatcagggat
     2341 tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc ctcacggtac
     2401 catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata aattcgatgt
     2461 tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag gtgagttgca
     2521 gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa atgtacatcc
     2581 agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt ttcataatca
     2641 gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct tccatttgat
     2701 ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg atcatgatcg
     2761 tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt tgaatcagcg
     2821 atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca tgaaagaaat
     2881 cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg agcttggtat
     2941 ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct cttacatggg
     3001 tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat tcgaatttgt
     3061 ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag aattgaacac
     3121 aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa tgatggaatg
     3181 tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc tcttttttga
     3241 agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa aagttcaggg
     3301 aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga aaagcagcca
     3361 gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga attagtcaag
     3421 aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg cacgattcat
     3481 catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt acttccaggt
     3541 ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga agaagctgct
     3601 gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa tgatgaagtc
     3661 attatctcta cactgacaat cggaatctta gcagatacag cagtttggat cagtcaagaa
     3721 gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt attgctgaag
     3781 aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg aaaatcccaa
     3841 ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga cgactcagcg
     3901 accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat tttccgattt
     3961 attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg cattacttat
     4021 ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac tcgaacggat
     4081 ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg attaacagtt
     4141 tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag ttggaagaag
     4201 agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg tgaattttta
     4261 cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct ggcgtgttcc
     4321 tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat tgatctctcg
     4381 aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg ctgcaggtgt
     4441 acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg tcgtctaaag
     4501 gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt gccattattt
     4561 attatttaat caatcaatca tttgcagata tctttgccga attgctgtct accagtgttg
     4621 ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa gggcgctcaa
     4681 tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg ttcttgacct
     4741 ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg atctcggaga
     4801 tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg acagcactcc
     4861 atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg gtcatccaat
     4921 tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct ggtatccttt
     4981 tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat gttcaaatat
     5041 tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa tgggtggata
     5101 catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc caagacagga
     5161 ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg tatgtacttc
     5221 cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc atttcgttta
     5281 tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt ggttcctttg
     5341 aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca gtttcttcgc
     5401 ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt gtgtatgtga
     5461 ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa gagaataaag
     5521 aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa gaaaaacgtt
     5581 atggataaga taattttacg tttgtttggc tcttttttta tatacataca atcaattttg
     5641 tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt atattttgac
     5701 caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa gtgatcttac
     5761 ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac gcttcaagaa
     5821 gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat cgacattgcc
     5881 catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta tacggatgcc
     5941 ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga gtatccagtt
     6001 gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt tcgattattg
     6061 aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac ggaagtcgtt
     6121 attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa taaaaacgga
     6181 ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga aagagtgacc
     6241 tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga tttagtacag
     6301 caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat ccgtgatgtc
     6361 atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga acccggtgta
     6421 gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga tgtgcctgaa
     6481 aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc tggagcaaaa
     6541 ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa aaaaagtgat
     6601 ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg aaaaacagaa
     6661 ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga attacatttg
     6721 atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa agcgttggaa
     6781 cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat cagtattctg
     6841 cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga caacgcatta
     6901 gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc ggacaaagca
     6961 atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc tatgccaacg
     7021 gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc tgcactgaaa
     7081 aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga attagctgaa
     7141 cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga agaagtcaat
     7201 tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga agatgcagaa
     7261 aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc tcaattagaa
     7321 aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat ggttcaagag
     7381 tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat ccccgtaacg
     7441 aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact tcataaacga
     7501 gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg ttcacgggct
     7561 ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc aactggtgtc
     7621 ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga agatcatatg
     7681 gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct agtcggagct
     7741 cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt cagacgaaat
     7801 ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt cttcaatatc
     7861 ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt agtagatttc
     7921 aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct ggaaggcgtg
     7981 actgctgatg gaacgattcc agaagcagtg gctgaacaag tcaacacgct attacgtgga
     8041 aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac accattgagc
     8101 ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca acgtctggaa
     8161 catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc tgaaaatgct
     8221 tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga agtagaaaca
     8281 ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt aacgattact
     8341 ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt ctaatcttcc
     8401 attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct ccttttttca
     8461 aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc ttgaagctgc
     8521 ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt tataactttc
     8581 aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta aggcagcacc
     8641 tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc cgacaaacag
     8701 tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt cgcataagaa
     8761 ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga ataagaggaa
     8821 caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa tcaatccata
     8881 gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc aaaaagagat
     8941 gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga aaaaccagta
     9001 aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga cttgtggcat
     9061 gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt gagaagtcga
     9121 atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac caaaagcaag
     9181 taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca tctaaaaatc
     9241 tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc aatttgatca
     9301 aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct tgtagtgcag
     9361 cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag ccgatcactg
     9421 ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg atcgtcagcc
     9481 catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca gcaagagcaa
     9541 tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct tgaggaagat
     9601 agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg ttatataaca
     9661 aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca tcatcctttt
     9721 tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc tagtgacttc
     9781 agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc acggcgataa
     9841 ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc tataggttca
     9901 tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc attattccaa
     9961 ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac aaacggaaac
    10021 cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc gatctgctct
    10081 ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat atctccatgt
    10141 acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt tcgagcttct
    10201 tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca gtagaacaga
    10261 acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc atcttcattt
    10321 tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc atggttttgt
    10381 tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa tcgctcaacc
    10441 ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa ggaaagttta
    10501 tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg atacaaataa
    10561 gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag atcttgcatc
    10621 aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc cattttataa
    10681 cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat cagtttattt
    10741 tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga acggataaaa
    10801 gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg gaacacatag
    10861 gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa ttcaagattt
    10921 tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat aacagtatag
    10981 caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta aattactgct
    11041 tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta tggatataaa
    11101 gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc atatcgacca
    11161 tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga cgtctcgcga
    11221 aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa tcacgattaa
    11281 attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata tctttcactt
    11341 gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc ttgctgcctg
    11401 tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa cattggccaa
    11461 tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata aaatcgacct
    11521 acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg gaatcgatgc
    11581 tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata ttctcgagca
    11641 gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa aagctttgat
    11701 ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga tcacggacgg
    11761 tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat ttgatgttac
    11821 agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg tcggtgatgt
    11881 cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg atacagtaac
    11941 gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga atccgatggt
    12001 ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg aagctttaga
    12061 aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc aagcattagg
    12121 ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc aagaacgctt
    12181 ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt accatgttaa
    12241 taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg aacctgtaac
    12301 gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa atgattttgt
    12361 cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga tggattattt
    12421 agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg tctttgactt
    12481 tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg aaatgtctgg
    12541 ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa aagtcgatgc
    12601 attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa tcgttgaaaa
    12661 actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag ctattggaca
    12721 aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg ctaaatgtta
    12781 tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag ggaagaagcg
    12841 gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg ttctgaaaat
    12901 ggacgaggac gaaccgaaga aatagcaagg gtttgtttga aaacagtgta aaaaataaac
    12961 catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DARBEG010000078        12943 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000078 DARBEG010000000
VERSION     DARBEG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.079"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCK20_002184"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCK20_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG1146217.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="SCK20_002185"
     CDS             complement(1195..3036)
                     /locus_tag="SCK20_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG1146218.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="SCK20_002186"
     CDS             complement(3218..5533)
                     /locus_tag="SCK20_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1146219.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="SCK20_002187"
     CDS             complement(5718..6578)
                     /locus_tag="SCK20_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG1146220.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="SCK20_002188"
     CDS             complement(6637..7158)
                     /locus_tag="SCK20_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG1146221.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="SCK20_002189"
     CDS             7436..8197
                     /locus_tag="SCK20_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1146222.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="SCK20_002190"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="SCK20_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1146223.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCK20_002191"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCK20_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1146224.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="SCK20_002192"
     CDS             12557..12913
                     /locus_tag="SCK20_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1146225.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         3713 a         2708 c         2300 g         4222 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaa
//
LOCUS       DARBEG010000079        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000079 DARBEG010000000
VERSION     DARBEG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.080"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCK20_002193"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCK20_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1146226.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SCK20_002194"
     CDS             complement(322..891)
                     /locus_tag="SCK20_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG1146227.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SCK20_002195"
     CDS             1077..1979
                     /locus_tag="SCK20_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1146228.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCK20_002196"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCK20_002196"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG1146229.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCK20_002197"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCK20_002197"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG1146230.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCK20_002198"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCK20_002198"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG1146231.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SCK20_002199"
     CDS             8547..8732
                     /locus_tag="SCK20_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG1146232.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SCK20_002200"
     CDS             complement(8794..9990)
                     /locus_tag="SCK20_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1146233.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SCK20_002201"
     CDS             complement(10160..11596)
                     /locus_tag="SCK20_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1146234.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SCK20_002202"
     CDS             11889..12698
                     /locus_tag="SCK20_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1146235.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARBEG010000080        12178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000080 DARBEG010000000
VERSION     DARBEG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.081"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(140..304)
                     /locus_tag="SCK20_002203"
     CDS             complement(140..304)
                     /locus_tag="SCK20_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146236.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            599..1927
                     /locus_tag="SCK20_002204"
     CDS             599..1927
                     /locus_tag="SCK20_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG1146237.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2129..2962
                     /locus_tag="SCK20_002205"
     CDS             2129..2962
                     /locus_tag="SCK20_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG1146238.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2978..3715
                     /locus_tag="SCK20_002206"
     CDS             2978..3715
                     /locus_tag="SCK20_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG1146239.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3870..4838)
                     /locus_tag="SCK20_002207"
     CDS             complement(3870..4838)
                     /locus_tag="SCK20_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG1146240.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5070..5903
                     /locus_tag="SCK20_002208"
     CDS             5070..5903
                     /locus_tag="SCK20_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG1146241.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5937..6776
                     /locus_tag="SCK20_002209"
     CDS             5937..6776
                     /locus_tag="SCK20_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1146242.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6802..7362
                     /locus_tag="SCK20_002210"
     CDS             6802..7362
                     /locus_tag="SCK20_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1146243.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7561..9282
                     /locus_tag="SCK20_002211"
     CDS             7561..9282
                     /locus_tag="SCK20_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG1146244.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9447..10589
                     /locus_tag="SCK20_002212"
     CDS             9447..10589
                     /locus_tag="SCK20_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1146245.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10605..11816
                     /gene="thiI"
                     /locus_tag="SCK20_002213"
     CDS             10605..11816
                     /gene="thiI"
                     /locus_tag="SCK20_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG1146246.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4152 a         2042 c         2528 g         3456 t
ORIGIN      
        1 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
       61 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
      121 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
      181 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
      241 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
      301 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
      361 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
      421 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
      481 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
      541 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
      601 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
      661 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
      721 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
      781 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
      841 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
      901 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
      961 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     1021 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     1081 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     1141 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     1201 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     1261 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     1321 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     1381 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     1441 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     1501 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     1561 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     1621 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     1681 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     1741 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     1801 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     1861 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     1921 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     1981 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     2041 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     2101 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     2161 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     2221 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     2281 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     2341 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     2401 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     2461 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     2521 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     2581 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     2641 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     2701 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     2761 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     2821 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     2881 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     2941 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     3001 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     3061 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     3121 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     3181 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     3241 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     3301 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     3361 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     3421 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     3481 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     3541 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     3601 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     3661 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     3721 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     3781 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     3841 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     3901 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     3961 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     4021 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     4081 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     4141 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     4201 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     4261 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     4321 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     4381 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     4441 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     4501 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     4561 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     4621 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     4681 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     4741 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     4801 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     4861 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     4921 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     4981 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     5041 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     5101 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     5161 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     5221 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     5281 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     5341 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     5401 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     5461 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     5521 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     5581 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     5641 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     5701 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     5761 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     5821 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     5881 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     5941 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     6001 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     6061 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     6121 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     6181 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     6241 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     6301 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     6361 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     6421 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     6481 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     6541 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     6601 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     6661 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     6721 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     6781 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     6841 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     6901 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     6961 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     7021 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     7081 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     7141 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     7201 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     7261 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     7321 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     7381 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     7441 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     7501 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     7561 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     7621 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     7681 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     7741 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     7801 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     7861 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     7921 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     7981 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     8041 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     8101 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     8161 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     8221 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
     8281 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
     8341 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
     8401 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
     8461 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
     8521 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
     8581 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
     8641 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
     8701 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
     8761 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
     8821 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
     8881 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
     8941 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
     9001 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
     9061 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
     9121 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
     9181 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
     9241 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
     9301 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
     9361 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
     9421 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
     9481 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
     9541 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
     9601 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
     9661 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
     9721 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
     9781 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
     9841 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
     9901 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
     9961 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    10021 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    10081 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    10141 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    10201 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    10261 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    10321 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    10381 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    10441 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    10501 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    10561 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    10621 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    10681 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    10741 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    10801 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    10861 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    10921 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    10981 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    11041 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    11101 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    11161 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    11221 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    11281 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    11341 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    11401 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    11461 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    11521 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    11581 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    11641 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    11701 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    11761 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    11821 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    11881 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    11941 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    12001 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    12061 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    12121 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DARBEG010000081        11783 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000081 DARBEG010000000
VERSION     DARBEG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.082"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..331)
                     /locus_tag="SCK20_002214"
     CDS             complement(152..331)
                     /locus_tag="SCK20_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146247.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(712..1419)
                     /locus_tag="SCK20_002215"
     CDS             complement(712..1419)
                     /locus_tag="SCK20_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146248.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1412..1849)
                     /locus_tag="SCK20_002216"
     CDS             complement(1412..1849)
                     /locus_tag="SCK20_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146249.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1864..2115)
                     /locus_tag="SCK20_002217"
     CDS             complement(1864..2115)
                     /locus_tag="SCK20_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146250.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2115..2321)
                     /locus_tag="SCK20_002218"
     CDS             complement(2115..2321)
                     /locus_tag="SCK20_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG1146251.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2758..3018)
                     /locus_tag="SCK20_002219"
     CDS             complement(2758..3018)
                     /locus_tag="SCK20_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146252.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            3093..3296
                     /locus_tag="SCK20_002220"
     CDS             3093..3296
                     /locus_tag="SCK20_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG1146253.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3437..3922)
                     /gene="ssb"
                     /locus_tag="SCK20_002221"
     CDS             complement(3437..3922)
                     /gene="ssb"
                     /locus_tag="SCK20_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1146254.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3925..4824)
                     /locus_tag="SCK20_002222"
     CDS             complement(3925..4824)
                     /locus_tag="SCK20_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG1146255.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4837..5430)
                     /locus_tag="SCK20_002223"
     CDS             complement(4837..5430)
                     /locus_tag="SCK20_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG1146256.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5506..5655)
                     /locus_tag="SCK20_002224"
     CDS             complement(5506..5655)
                     /locus_tag="SCK20_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146257.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5671..7875)
                     /locus_tag="SCK20_002225"
     CDS             complement(5671..7875)
                     /locus_tag="SCK20_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG1146258.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7886..9346)
                     /locus_tag="SCK20_002226"
     CDS             complement(7886..9346)
                     /locus_tag="SCK20_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG1146259.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9407..11779)
                     /locus_tag="SCK20_002227"
     CDS             complement(9407..11779)
                     /locus_tag="SCK20_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146260.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2923 a         2464 c         1981 g         4415 t
ORIGIN      
        1 aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc ataccgaacg
       61 gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt aaaagtgcta
      121 tcacttttag tttttaggat aaaaagtgac gtcacttttt gagtgctggt acctcaaatc
      181 ctgctttttc ttgggcaatc aataaatcta acgcaataca cataatcctg gattcacttt
      241 tgccgtactt ctgctttagt tgctgcaatt ctttttgtct cttttcatcc aacgtcacgg
      301 gtaaccgttt atttcctatt gcaattggca ttagtactct ctcctttcat cgaaataacc
      361 ctattaaaaa tatggctgca atcaacttga atattagact cacaagggtt aacacaaagc
      421 gccaaaagat caaacaaaat accagtattc ctaagaatac tacgaagttt aaatgtagag
      481 ctgcaccatg acgcataagt atctcgccaa caaacagtac gaacaataga ggaatgattt
      541 tattcatatg gatctccttc tttctcttag gttatcgggc tttgcccgct tatttcgcac
      601 cacctaagcg gcgggggcgg ttggatcgat tggtgcgaaa taagaaccat tcccttaatt
      661 tcaaggtttt ttgttcttat ttcacacatc ctcacttttc ggtataaatg gttattgacc
      721 ttttttcctt tcaaatcgtt gatatggcat tcgtttacgg tcaagttcat tttggatttc
      781 ttctgtatcg tcgtaagtgt aggtgtctaa aaaatcagtt aaagtcgtgt accagtcgtc
      841 gcctgggtta tctacttctt ctacatattc cctcaaaaat gcttcgggta cagtgagttc
      901 ataatccatt cccattaaca cgatagattc cgtcccacac gtcaaaaatc tagtaaatac
      961 aaggtaataa tcgtctaccg tcgcatcttt gctgtttagc tcttccattt cttcttccat
     1021 taattccatg ctctcaaaaa ttgaataata ttctgattct gagtaatagt gtttcctgca
     1081 aatcgtgtag ctgacaaagt tttcgaccag ctcactcctt tctaggtaaa cattcatgat
     1141 atatttttct gtttctagtc gtagaagctc cgctcctttt tttaatgtgt caggacgacg
     1201 tttatacacg tctaaacgat tatggctgac tgtaaccttc gtttgactcg tttcactttc
     1261 catatacaac tgttcttcca ctctttgaat aaagtacttc atttcttcca tacaactaaa
     1321 gtctttttga atccgtctga ttacttcatt caagtgttct ttgtttgtta aaaattgcga
     1381 aacatcttct gttgttagct gataaaccac cttattcatt atctttctcc tccgattcaa
     1441 tcgtataaaa tgcaccattt ttttcgatat tttcaaaagt atctagccag tcgctagcca
     1501 ttgctttagc tttcttaatc tgtgcgaatt ttgcataagt cagttctaag gcttgatatt
     1561 cggaaggttt gatagtgctt ggtttgacac ctacttccaa gcatagtaag gcttggaaaa
     1621 accattttcc attttgtttt ttcagttctt ccagtgttcg atagtacgtc gcaatgagat
     1681 tcatttcgac acctgattca atcatttctt ccactgcgac tgacaatttt tccttgtaat
     1741 gttttttggg tacacgtgtt tcatggtata ggaacgtcat atcctccatt ttttgtgcca
     1801 ttttatccaa aggtaatttg acaaattcgt catagtaatt ttgagccata ttttttctcc
     1861 tttttagttc tctaatttgg ttaaccaata ttcgatttca taagtttcca tgttttcttg
     1921 ataatgcagt tcatatcctg caatcttcac taggtcttca aatagcatag tcggtgcatc
     1981 ctctactaaa ggggtttcta tcgtccagta ccgcggaata agtaaagctt ctgctacttt
     2041 ttcagaaatg acttctttgt ccaccaaaat ttgaacgact tcttttacag tcaataattt
     2101 gtgttgtgtt ttcatcaccg tttctttact ttctcatcaa tcaaaacata atcgccataa
     2161 gacaaggtat attttttccc gcatgttgga cattgataca aacgatagct tttgatacat
     2221 ttcgctcttt ctccacaatc gcattcgaca gtatgtggtg catgatagtt tgggaataat
     2281 tcgctgaagg gaacacccca ataatcaaat ttctttcgca tgattgattc tgcctcctct
     2341 taggaaaggt tttatttcct ttcgttagtt ctttctttat tgatttgttg gccagaatga
     2401 atcaacgaaa agcgacaagg acaagggcgc gcagcgtgcc atttaccctt gtccttgtcg
     2461 ctttttggtg ataaaaggca acttgttgcc ggtctggcaa atcaataaag agatagcgaa
     2521 caggaacgaa ccttttggtg agaggaagtt ttttctattt tgtcttgctt tgcaagacac
     2581 catatcttag tgaacgtgca acgttcactt cctttcttct cgaacggcgg tggcggttgg
     2641 agtgagagaa aggtttttca attgagaagg cacttgtgtc ttcctaaaaa ggtcttttcc
     2701 tgtttctatg taaaacaaaa agagctgcaa tcctcattta ggactgcagc tcttctttta
     2761 tatgcccatc gatcgcgtaa aatgatcggg gtttctaatt tgcatcgatt caatgactaa
     2821 ttgattccgt ttattgaaat gacctgtgac ggcaatattg tattttccat catccataat
     2881 taacaattta tcgataattt caatatctgc aactacacaa ttaatcgatt catgaattgt
     2941 ttgtaaagta aagcgaacca ataatggttg tgtttttatt actcggattt tatcaagcaa
     3001 accaacatat ttgttcatca cgatcacctc gctaaaatta tagcaaaaac agctaaaatt
     3061 gagaattttt attcgatttt agatatgcta taatgatcaa gacagtaaag tttgtgacgg
     3121 tggctatccg attgaaagtg aggtggtgcg tatgtgcgta cttccaaaat cttacagaaa
     3181 ggagaagcct tttgcctgtt gaaacggcgc taggattaat gattagtttc ggttctttga
     3241 tcgcaacaat catttttgga atcttagcag ttgtaaacaa caatcaaaaa aaataaccgt
     3301 cagctttagc cggtcgacga ttatttttca aaaataatat taagctggtc accgtcttaa
     3361 acggtgctgt taggggagtt gttagcgcaa ctcctctttt cgtatccatt ataacaactt
     3421 tattgatctt ttacaatcag aatggtaaat catcatcact aatatcgatt gattgtccgc
     3481 caaatggatt ttgtgaagcg ttcaatccat tgttgttagg cgtagctaca tcccgttgtt
     3541 caaaggtggt agggcttgga ctgctggacg attgatcagc gtgcgcacgg ctttctgtcg
     3601 ctgctttact ttctaatagt tggaaattat cggccacaac ttctgtcaca tacacacgtt
     3661 gtccttgttg gttatcgtag gttcgggttt gaatccgtcc aacaacgccg atcaaactgc
     3721 cctttttagc taatgtcgcc atcgtttctg ctggtttgcg ccaaatcaca cagttaataa
     3781 aatcagcttc tcgattgccg ttttgattcg tgaaatttcg gttgacagct agggtaaaag
     3841 tcgctactgc agtaccactc gcggtataac gtaaatctgg atctttggtt aagcgtccta
     3901 ctaaagttac attattgatc atctttatct ctccatttct tgattcattt gttgtgatct
     3961 tttaaactgc cgttttcttt cttgattttc aattttgatc acacttttca ttaggtgatc
     4021 aatttcgttg actgtatctg taaatgaaat ggaattcgca aacgtgttaa ctgagcgata
     4081 tttttcccac tgcttttcca aatagtcatt tcctctgatc ttatccattt ctctttcata
     4141 ataaaaggac aacggttttg tatccccacg ttttgtaaat gtatttcgaa cacttttgta
     4201 taactctttc gtatcgatat tttctagttt cggaatggta aataagtcgt agtaatcttt
     4261 tgctctcgaa ttatctgtgc caaagctgaa cgtagcactt agcttttctg caataatttg
     4321 ttctgttgga taagctggaa tctccacttt tttatcttca aacattaatt ggtgatagtg
     4381 tgtatctact tcaggataaa tagaatctcc tgttgtcacg tccattttaa acggtacacg
     4441 tactttctct aaattagcca ccaatttaat ttgaaatcca ggataaaaat cggcttctct
     4501 tgtttccttc attccttgaa tagagaaaac aatgccttct tttgtgggtt ttctagttaa
     4561 atcattaaaa atgctcttca atttttcttc agttaatttg ctatttctaa atgtcgtatc
     4621 gatatcaatt gttgtccttc tatcgattcc atattttgat cctaataaaa agccgccttt
     4681 caaaatgaag tcatttttgt actttgattc agaaatttgt tcaattagac gatccatcat
     4741 aaaatgccgt tgcactaact gtgcatttaa tcctgtactc tttgctaaat ttcttgtttt
     4801 tgctgcaaat tgtgtcggtg tcatcttttt cctccatcaa tataagcttt gcatgtggta
     4861 tttcatttct ttttctacat gtagttttgt catgtaatct cttaatttac ttggatctct
     4921 caatgggtct tccatgtact cttttaatgc ttctaatttc atttcatatt caaattcata
     4981 tctcggattc caaatatcac aaagtgtacg ctctttatca tacatagtaa taggatttcc
     5041 ttgaaaggaa gttccctcta cttttcctag ttcataaact tctttcttca cgaaatgaaa
     5101 ttgtaactgc tgctcttttg taattgaacg atcaacgtta tagccatggg gaaaagttaa
     5161 gtgaatgaat ttcggcatat tcgtagaaag ctggtaaata tctaaagtcg tttcatgaga
     5221 gaagattccc ctggaaaatc gttgctgaag ccaaaataaa tcatctattc catgaccatc
     5281 ctctacataa taaccacgat caactttgat caattttcct tgattcacca atctttgtac
     5341 agtggttgct gcgattccaa atttcctggc ttccttcata gataaatttc catcgcgaat
     5401 agccaacaat tctttcagtt tatcttgcat ttacttcccc tcccttcgtt acaaatacac
     5461 tacatcaaat atatcatagt agtttatttg taacaagatt aacgtctatc gatctaattc
     5521 gatttctttt tgaatgcgct gctggttctt taattgttgc agtcttcttt tatttgcacg
     5581 taatttctct tgttctaaac gaatttttgc tttttgattt tcgatttctt gttgtttgtg
     5641 tgttgtattt cgcataggat aaccccttca ttattttgta gtgcggcgtg ttcttttctt
     5701 atagttcgtt tttttctttt cagaaaattc aaagcctgtt gtaaagtctt cttttaaaac
     5761 gatcgttgct tcaaacgaac ctttctgacc ttttagtcct ctgactgatt ggcttgttcg
     5821 gccgttttga atgatctctt tcagttgtgt ggctgtgagt ttctttttgg caatcgttgt
     5881 ccataattta aaatcacagg cttcagacgt acatgccgcc acattttgat aaagcaagac
     5941 ttcttgttcc cgacattttg gacaaacacc taaatgacgc gttgcttcct gtttttcact
     6001 ggctgctttc acctcttgta acgttgatcc aaaatctgtt gactggattt gtcccggtac
     6061 gacttccaac aagtgagaga caaatttttg gatattagct aagaaagtag cttgtgttcc
     6121 tttctgctca ccaatcttta ataagtaact ttcccatttg gcggtcattt cggcacttgt
     6181 tagcagcggc tctttttcca cggcttggca aagtaaaatc cctttatttg tcaccacaag
     6241 cttatttttt tctaccttga tatattcctt ttgtttcaaa gtttcaataa tatttgctcg
     6301 tgttgcttct gtaccgattc cttctacttc tttcaatatg gattgtgctt cttcgctatc
     6361 cacggttttt ccagctgttt tcatcgctgt gatcaacgta ccttcggtat aaggttgtgg
     6421 tggcttcgtt tctttttcaa ggacttgtag atctaccgtc accgcttctc cttttgtcac
     6481 tttaggaagt agcggttctt cttccttttc ttttctgtcg ctctctttgc caaacagctt
     6541 tttccaacct agatctactg gtacttttcc gactgtcttt agctgaagct gctgaacttg
     6601 cgtcaagatc gttgtttctt cataggtata cttttccgca aacatggcca ccgttgtttt
     6661 taacacttgt tcataaatag cctgctgcag tggacttaat tgttcaaaag ccgctgccgt
     6721 catgaccgtc tttgtaggaa tgatcgcgta atgttcctgg actttcttat tattcacata
     6781 tcgactattc ggtgtatgaa taggagtagg aatggcttcg gcctttaaaa agtgtttgta
     6841 ttcgtctaag tgatccaaca gatacgcata ctccccttct gtaatataag gggtatctgt
     6901 tcgtgggtag cttagatatt tcccttcata caacccttgc atggcttcca aagtatcttt
     6961 ggcactagct ttcatgagct gattcatttt agattgaagg ctggatagag aaaaaagacg
     7021 tggacttgcc gcactttttt tctcggtttc aacttggtgg atcgtacctg gctgttttcc
     7081 caattgaacc cccttttctt ttaaataatc ttcaaatgct tcttgcgtcg cttgggtttc
     7141 attcggatca agcttcgcat caaatgcgcc attttgagcg attacttggg cttttccttc
     7201 aaaatatggt tctttttgga agtttctaat cttttcctgt aattgataaa tcatgtaaag
     7261 cgttggggtt tggactctgc ctaaggaaaa acttcctggt atgccttttt gctgcaagag
     7321 taaactatac agcgggctac cgttcatgcc gatcaaccaa tcggcgatct ggcgtgcttg
     7381 tgcttcttca aattttgggt aggtctcttc tgcatctcgt aactgctgga atccctgata
     7441 aatggcttct ttttctaagg agttgatcca aagtctttta aatatctttc catgatcggc
     7501 gcctgcttgt tgaatgatcg tccaagctat cagttcccct tcacgatcgc tatctgtcgc
     7561 aataataatg gtgtcggccg attgaagttg ctgtttcacc accttgaatt gctttttctt
     7621 atctttagga acatgatagt gataatgtgt agggaaaata ggcaaatgct ctaatgacca
     7681 ttgtttccaa ttttcatcgt acatatcggg cgaatctaac tcgactaaat gaccaaaacc
     7741 atacgtgatc acagtttctc ctgaaaacaa tcgatcttgg atctcaaaat acccatcttt
     7801 tcgggtagct ttagaaaagc tatcggcata agccttcgct tgtgacggtt tttctgctaa
     7861 aataacggtt ttcatcatga cgatttcact ccctttcttg ctgtctacct tgttgaaaca
     7921 acatggcacg tttttgcttt ctgatctctt gttggtagat ttcatgcttc aattgatcat
     7981 tccaatcctt ttttacttca ggtacaaatt catgttcatg atttttatag cctttgtagt
     8041 attgttctgc tttataagag aaaagaatct gagcattaat atcccctttt tcttgataaa
     8101 aacgatcaaa atcgtacgta ccatccacgc gttcacaagc ttttaattct tttgcgactt
     8161 ctctacaaat atctttcaaa ttaatagctt ctattgaatc gttaacagaa agctgctgac
     8221 caaaagacgc atttactttg tgagcggaaa ccaattggtt cgttaattgc agattgtttt
     8281 ctgtcttgtg tatggccata atagcttctg gtgtgatctt ttcttctttt gcgacttctt
     8341 cataaatgac tttgtattct tttggcactt gccaatcatt agcgattcct cgttcacaac
     8401 gtactttctg attcgtaaat ggatcaactg cgcccattaa ttgttctttt tcgtagaaaa
     8461 tatgtcctgc acggtcatta tccacacaaa tctcgatgga ttgaggaaag gcttgtttct
     8521 ttctcaattc agatactgcc tcaccaaaat aatgactgat cacatggtgt tttaaccctt
     8581 ccatcgaaac taaccacgta tcgtttaact gatcacgatt tagtgcagca tagctaagta
     8641 aatcaatact actctcaaaa aattttaact gcttagggtc accgatcttt aggttaaagc
     8701 catgattcgc tgtcgaattt ttatctatat gcttatatgt gcctctctca ccaaaacgtt
     8761 cataatcaat gaacgttcct tgataactcg cccctactgt ctcatttgtc tgtggatctt
     8821 tccataaaaa ggcgaccgca tgatctaaac gatcgtctcg aactaagaaa ttttgccgtt
     8881 catgttttgg ctttagttcg gcaatcaacc cttctttttc tgcataatca atcagccact
     8941 ctggtagttt tcgttcttcc ttcagataat tccttccttt ttctgtgagt ggttgataat
     9001 caacggggtg atctttgtac ttttctgtct caaaatgaag attttccact cgttctgttt
     9061 tgtcttcatt ctttagaata aaatcagtgg ctgctttaaa ttgttgctta gggtcttgga
     9121 tctctttgtc tatgaaaaaa agtttagcta attcaataat gtcgccacta atattttgac
     9181 tgttccaata aaaacgttgt tttcttcgat caaaaacaaa tgaatcatgg tcttctaagc
     9241 gatagtctcg tcctttattg actacagcca ttccattgtt tcgagcaaaa tccactatat
     9301 cgatctgttt acatcgatca ataagactca tttttttctc ggtcatttta aaacctccca
     9361 aaagaaaaaa gactatccgt ttttcagata gtctttttta cttgttttat acacgttctt
     9421 tttgtgacaa ttgttgttca taatagattt cattttttcg ttttgccact tctgacactg
     9481 tctcaagtga aaatcggaat gaatttccta gcgcaaattc ttctgaaggt aacgccgttt
     9541 ggatctcttt tccataactt tttcctttta aaattacttg attatgctgt ggatcttcaa
     9601 taaacgccgc atccttcaaa tgtaacgttc gacccgtatc ttcttccatt actttcccgt
     9661 ctttcttgtc gatccaaagt gaaccaacag gaacaatatt cttttgttct gccgtttcta
     9721 cagtattttt atgctcctgc ttcacttctg ctggttggat ctctggttct ctttctctgt
     9781 attgattgat ttcattagaa atggtttgaa tgaattcatt tgcgacacgg ctgacatcat
     9841 tcatgatttt cccacgttcc gcagcttcct tatcatccaa cgtctgacca ttcttcgtcc
     9901 atgctgcaat ataaggaata gctttctccg aagtgtccat tccaaaatgt ttgcacacaa
     9961 tataagaagt catttctgcc tgcaattctt tagttgcggt atctaattct gaaaatttgt
    10021 gcattcttgc atgggctaat tcatgaatac ttgtagctaa atgctgcgta ggagtatttc
    10081 gagtattcat gacgatttct tcttcgccag taaattcgtt acgaacatat gccccccgtg
    10141 cttgtcctaa ttctctttga tagacttcac tttctgtcat atctcgaatt tgtattccaa
    10201 tattgttggc taccgcttga atcccagctt gaagttctga ctgtcctttt ttatccaatt
    10261 gaaaatcaaa gacacggttc ggaaagattt tcggatagtc ttcaggtttt aactgtgttt
    10321 gtgtgatatc aaacactttt tcaatcgtgt agtaaggttt tttgatcact tcgatttgac
    10381 cactttcgat tcgtttttta tcttctggtg tagcttctgt ttctcgaaca aagccacgtc
    10441 ctttctcata aaatcctttg accgtttttc tgacaaagat cttgttccct tgttcccctt
    10501 ttttgatata gattccttct tttttcaacg cagcacgacc taaacaagcc gttgcacctg
    10561 gaaattgttc ctcaatcaat aattgatttc taaaggatcg ttctggaaaa cgagacataa
    10621 aattcaaaag ttctttcacg tcttcaggag aatgggtatg ctttttcacc gcatccagtg
    10681 cctgttgcga aagggatttg acttcttgct taatctcttc tgccgttcgt ttttgatagc
    10741 ccttttgttt tgtttttgtt tctttcgact gcatttgttc tttcgaaaca ggttcatttt
    10801 tgaccgtttg tttttgttcc ttttcaggta cttgttcgca caattttgct aactctaaat
    10861 agatgggttg attactgttg acgccatccc ctagatccat tcggatctga tcgactactt
    10921 ctccgttctt cacttcttca aaatagaatt tataataacc tgcttctgcg gcgtattggt
    10981 tatccaacgt tttgagttga tcgatcaatt ctaatgttac gatctcccca ccataacttt
    11041 tcaatccttt ttctggatca ttttcgttga attctactcg atagaaatga tcgttttctt
    11101 tctccttcaa agaatcagtt ggcgcagcct cttcaagtaa ttcacgaaca gctgcttttt
    11161 cctcttccga cgtggcttgg tatttttttg attcctcaaa tcccccgttt tcattcagtt
    11221 ctaaagcaac ttttcgatag tctttcaaaa aataaatggt tggatttttt ttccattgcg
    11281 tcactcgtcc gctgtcaata attgcttgta cttcaggaag aagattttct tttccttttt
    11341 ctgatctttc tttcagctgt tctaatgttt ccaatttccg ttggtatttt cggatcgtag
    11401 cggctgaaaa agtcgattca cctttttcaa agcgttcgat ttcttctttg attcttggaa
    11461 tattttcgat tgtcatatcc aggccgccgt acttgttata gcccatttcc tttagctctt
    11521 cttgtctttc aagtttttct acctgttttt cttgttgctt gatctcttct ctcagactat
    11581 gcactttgtt ttctaaacgt tcttgttgcc taaaccaaga atgcccattt ctcttatcgt
    11641 tcattggctg accgttggtc tttctctggt gttcaaacat cttctcaatt tgttcgttta
    11701 attcattttg caatcgtact aattttcttt tggattttcc caagcgacta ttgtctagtg
    11761 gttctttttc ttctgccatg tcc
//
LOCUS       DARBEG010000082        11727 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000082 DARBEG010000000
VERSION     DARBEG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.083"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..629)
                     /locus_tag="SCK20_002228"
     CDS             complement(105..629)
                     /locus_tag="SCK20_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146261.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCK20_002229"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCK20_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG1146262.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCK20_002230"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCK20_002230"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG1146263.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="SCK20_002231"
     CDS             complement(2351..3031)
                     /locus_tag="SCK20_002231"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1146264.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="SCK20_002232"
     CDS             3157..4026
                     /locus_tag="SCK20_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1146265.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="SCK20_002233"
     CDS             4052..4567
                     /locus_tag="SCK20_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146266.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="SCK20_002234"
     CDS             complement(4614..5207)
                     /locus_tag="SCK20_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146267.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="SCK20_002235"
     CDS             complement(5232..5411)
                     /locus_tag="SCK20_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146268.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="SCK20_002236"
     CDS             complement(5488..5997)
                     /locus_tag="SCK20_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146269.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="SCK20_002237"
     CDS             complement(6093..6473)
                     /locus_tag="SCK20_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1146270.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="SCK20_002238"
     CDS             6803..7552
                     /locus_tag="SCK20_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG1146271.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="SCK20_002239"
     CDS             complement(7736..9013)
                     /locus_tag="SCK20_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146272.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCK20_002240"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCK20_002240"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG1146273.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="SCK20_002241"
     CDS             complement(9935..10879)
                     /locus_tag="SCK20_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG1146274.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="SCK20_002242"
     CDS             complement(10881..11567)
                     /locus_tag="SCK20_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG1146275.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3350 a         2475 c         2054 g         3848 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct tttttta
//
LOCUS       DARBEG010000083        11581 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000083 DARBEG010000000
VERSION     DARBEG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.084"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(183..728)
                     /locus_tag="SCK20_002243"
     CDS             complement(183..728)
                     /locus_tag="SCK20_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1146276.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(740..1432)
                     /locus_tag="SCK20_002244"
     CDS             complement(740..1432)
                     /locus_tag="SCK20_002244"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG1146277.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1451..1735)
                     /locus_tag="SCK20_002245"
     CDS             complement(1451..1735)
                     /locus_tag="SCK20_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146278.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1748..2311)
                     /locus_tag="SCK20_002246"
     CDS             complement(1748..2311)
                     /locus_tag="SCK20_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1146279.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2539..3201
                     /locus_tag="SCK20_002247"
     CDS             2539..3201
                     /locus_tag="SCK20_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG1146280.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3325..4521
                     /locus_tag="SCK20_002248"
     CDS             3325..4521
                     /locus_tag="SCK20_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG1146281.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4622..5998)
                     /locus_tag="SCK20_002249"
     CDS             complement(4622..5998)
                     /locus_tag="SCK20_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1146282.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6035..6901)
                     /locus_tag="SCK20_002250"
     CDS             complement(6035..6901)
                     /locus_tag="SCK20_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG1146283.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6990..8015)
                     /locus_tag="SCK20_002251"
     CDS             complement(6990..8015)
                     /locus_tag="SCK20_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1146284.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8033..8707)
                     /locus_tag="SCK20_002252"
     CDS             complement(8033..8707)
                     /locus_tag="SCK20_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1146285.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8891..9784)
                     /locus_tag="SCK20_002253"
     CDS             complement(8891..9784)
                     /locus_tag="SCK20_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1146286.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9858..11555)
                     /locus_tag="SCK20_002254"
     CDS             complement(9858..11555)
                     /locus_tag="SCK20_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1146287.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3451 a         2524 c         1982 g         3624 t
ORIGIN      
        1 aagtgtattt tataaaaaga aaaaatataa cttaagttgt tgataataaa gagatttatt
       61 aacgtaaaaa gaataagttt ttttaaaata acgtataaaa aagagcataa aacaaagtcg
      121 tttgttttat gctcttcttt tattattttg tgctttttat tttatcaaat cactttgttt
      181 ttttataaaa tttctgcgcc tagtgtggac tcaaaatgtc tcaatgccca ttcatgtcca
      241 acgggatcga aacttgccac ggcatctttg aaaatatgga gtttgtatcc tagattatac
      301 gcatcgactg ctgtatgcaa cacacagata tctgtacata cgccggtcaa ataaatatct
      361 gtgatctggc gttctcgcaa acggatgtct agatctgttc cactaaaagc ggaataatgc
      421 cgtttgtcta tccaataaac attttcctct tgctcatagt cttggtataa actttttaaa
      481 gagccgtaca attcccgtcc acccgttcct atcacattat gcggaggaaa taagcggttc
      541 tccggatgaa aggaatcttt tggatcatgc gcatcaatgg caaaaacgac aaaatccttt
      601 tgatcaataa acttctttgt atgagcagtc agcttttttt caattacttg tcctgctgtc
      661 ccagtagtca gctttccatc ctctgcaaca aaatcatatg tgtagtcaat cgagatcaat
      721 gcgttcatct tagtcctcct tatacgagat gtttgacaaa ttcgatcacc atttctgctg
      781 atcgtttgcc tgcgtcttcg ataaattcgt caaagctttg tgtcgccgag tgatctgctg
      841 tgtcgctcat tgcacgtacg atcaaaaatg ggatattgaa ctgacgagct gtttgtccaa
      901 cagcggctcc ttccatttcg caagctaatg cttcagggaa attcgttaag atttctttga
      961 ttttatctgg cgagtcgaca aatgtatcac ctgtaacgat caatccttct tttgcgttca
     1021 aattcgttgc ttttgctgct ttaaccattt cactgcgcaa atatgtgctt gcttcataat
     1081 atagaggcat tcctggtaat tgtcctggtt tgtagccaaa tcctgttgca tctgcatcaa
     1141 aataagctac tttgtctgat ataacgatgt cacctacttg taagccttca ccgattccgc
     1201 cagctgaacc tgtattgatc accatgttca cgccgtattg ttggatcaac agacttgtgg
     1261 taatagatgc taagactttt cctataccgg aacgtacgac gatcacttcg tggttaccta
     1321 atgaacctga aataaataaa gcgcctgcgc gttcccatga taatggttcg cttaggttct
     1381 ctctcaaaat ttttacttct tcttccattg cgccgataat accgattttc atctttttaa
     1441 ttccttcttt ctatataaat gcgattgcta aaaaaaccac acataacatt aggataacaa
     1501 taataagaga tttcatcaaa aaagagttcc atttggtcgt cttttcccgt tcgctgatcc
     1561 ttgttttcgt gattggttta ttctttttgc tttctttacg ataaaagctg gcaatctttt
     1621 tttcttcttg ctgataagct gtttctgctt ttcgctgttt ttttaatgac tcgcttgctt
     1681 gtgcttgttg tcgcttgcga agttcgcttc gcgtaataag cggtcctttg gccataagcc
     1741 gatccctcta ctttcctttg gtcaatagtt cccagtattg aatcgcataa atcgtctttg
     1801 catcacaaat caactgatct ttcatcgctt gttgcgcctc ttcaagagtt agatgataca
     1861 actccagtac ttcgtcttca tcttgtgcta aaggattctc gaccttctct accccttgtg
     1921 catgataaat atgaaggact tcatttgcaa aacctggtga aagatacatc gaagtaagat
     1981 ggctcagcga cttcgcacga tagccagttt cttcttctaa ttctctagct gctgtcattt
     2041 cgggattttg tccttctcct ggatcgattt ttccagctgg aatctccaaa atcacttttt
     2101 ctaaaggttt tcgaaattgt ttgacaagaa gcagacgatc ttgttcgtca aaagcaatga
     2161 tcccgacacc accaggatga aataccagtt ctctcttcga agttcctcca tctggcaaac
     2221 gaacttcgtc tacagcaaca tcaataattt ttccgctata gatttctttg cgactgattg
     2281 ttttttcttc aaattgctca ttgttcagca tatcttctcc tgctttcttt gtaatcataa
     2341 ctgctttttt ttcatttttc aatttttctc aaaaactcct tattagttta ccatagataa
     2401 accttagatt ttctctaaat atagaaaatc cttctttagt ccgatactct ttctagtagt
     2461 ccgtatgcta aaataagtat gtaatcgaat aacgaaaaga attcaatact caacgataaa
     2521 tgacatggag ggtttattat gataaaacga aattggccgt ggcttctagc attggtcttg
     2581 ctatttctct ttgtccgaaa caatagtttt tcttttgttc taggactttt attgataggt
     2641 gtagttttag ttttgtgggg actctttggt tcgcgcagga aaaagggaaa acaagaagaa
     2701 attcctgcac tgactgacga acttgaaact cactatgaaa aaagcggtat gacagcgaat
     2761 gaaatttcat tttttcgaca aacaatgaat caaacaaaaa atgagatcaa tcaattgcaa
     2821 atgaatatgc agcagacagc caaacttaaa gcaatcgatc tccgccatga tccagtcaaa
     2881 gcagcgaaag ctttattcaa agaattggtg aaagaaccaa accgactgca tgaagcaagt
     2941 cagtttcttt atacgcatct gccgaatcta gttgatttga caaataagta taatgagatc
     3001 aatgaccatg agatcaaaaa caaacagacc tatgaaaaac tagaagaaag cgcgcagatc
     3061 atcgaccagc tgtcagcttt aatcgcacaa gattatcaaa aattcgttgc tgaggatcta
     3121 gatgatatcg acgtagagat atctgttgcg aaacagagct taaagcgtga taataaatat
     3181 gaaccagaac aaaaggatta actcttttgt tcataaaaga agactcggaa cattcagatg
     3241 atgtctggct gtttcgagta ctttttctaa agtagagaca ttcatgtaag aatcgagaac
     3301 agattcaaca aggaggaatt ttttatggat cataaaccga ctgaaacaaa agatgttaca
     3361 ccagttaacg atacacttga agatttattg aacaacccgt tttcaactcc aacagatacg
     3421 ctgaccactt cacaacaagc ggagattgat cagctccaag agcaacagac agctgctcgt
     3481 ttaattgaca aactaccggc ggaacgccaa gaacaagcta aacagctagc agcaaaaatc
     3541 gatgctaatg atgcacagtc tgtcatcagc tacggatcgg ctgcccaagc aaaattaagt
     3601 gaattctcac aatcgatgtt gaatcatgtg caagcacaag atattggtcc tgtaggagat
     3661 tccttaactg aactgatgta tcgtctccaa gaagccaatc ctgacgaact gcgtgccgga
     3721 gaagggaaca tcttccagcg tgtcttcggg aaggtcaaac aatcgatcta tgaagtgaca
     3781 gcaaaatatc aaaagattgg tgcacaaatt gacaagattt cagtgaaatt agataaagaa
     3841 aaagacggtc tgctaaaaga taatctgatg ttagaacagc tttaccagaa aaacaaagac
     3901 tactttgatg cactgaatat ttatatcgct gctggagaat tgaaaatgga agaacttcaa
     3961 acaacgatca ttccagaagc gatgaaaaga gcagaagaaa ctggcgatca gatggatgtt
     4021 cagattgcca atgattacac gcaattccta gatcgtctgg ataaacgtac gcatgatctt
     4081 cgcttagcta gacagatcac gatccagcaa gcaccacaaa tccgtttgat ccaaaacaca
     4141 aatcaggcct tggctgaaaa gatccaagca tctatcgcta cagccattcc gctgtggaaa
     4201 aaccaagtcg tgatcgcatt gaccttgctt cgtcaaaaag atgcagtaac tgctcaacgc
     4261 caagtctctg aaacaacaaa cgacttattg aagaaaaatt cagaaatgct gaaaatctca
     4321 gccatcgaaa cagcgaaaga aaatgaacgt ggaatcgtgg atatcgagac ccttcaaacc
     4381 actcaaaatg atttgatcga aacaatccaa gagacattgc gtatccaaaa agaaggaaaa
     4441 gaaaaacgtc gtcatgcaga agtagagctt ggacacatgg aagaagatct gaaacaaaaa
     4501 ctgttggatt taacccaata gatcaccttt cgcttgttac taaaacaaac gcccaacaac
     4561 agtttattac aactgttgat gggcgttttt cttatctttc ttctaacttc tccattggag
     4621 tctatttctc attcatcttg ctgtgtccat agccatagcc aaaataaatg atcagtccaa
     4681 taaccattac gataccgaac acgatccatg tcacagtttg cagacgaagc atcaatacaa
     4741 tacaagtcaa gatggagacg attggcaata gcggaacgaa tggcactttg aattctccag
     4801 cttttggttt gccaaacatt tttctcaact tcagtaagcc tagactaaca agcatcaaat
     4861 aggcgagcgc catgatattc gttagttccg ctaatacatt caatgggaaa aatccggcaa
     4921 agaatacagc tgccacacca gcggcataag tcgctttttt cggtgtacga tgcttttgat
     4981 cgatttctgt cataaactta ggaagtaagc catccttgct gacagcaaac agtaaacgag
     5041 caagtgaata catcatcgag atcgttaccg tcagtagcgt taaaattgcc cctacagcga
     5101 tcaccgcgcc cactgttcca tgtcctacat acctcattgc aaaagccact gggtcattga
     5161 ctcctagttc agtatacgga acgattcctg tcaagatcag cgtcacgatg atgtaaagga
     5221 cagtagcaat cagtatcgaa ccaatgattc ctcttggcac atctttttgc gggtttttga
     5281 cttcctcggc tgccattgca acggcatcaa atcccaagaa agcaaaaaac acaagagctg
     5341 caccatctag cacaccggaa aaaccaaaag gcatgaatgg ctgccagtta tcaggtttaa
     5401 cgaaaaatat tcctacaatg ataaacaacg cgatgatacc aaatttgaca tataccatgg
     5461 cattattcaa acgtaatgct ttcttggctt caagcgacac gatgtaagtg acgaatatca
     5521 ctacgagcac cgcaatcaag tcaatgtatg taccattttc cggattgtac ccgccagtta
     5581 atgcttgtgg aagttcggcc cctaaacttt tcaaaaaacc ttgcatatag ccagaccatc
     5641 cggaagcaac agaggaaacc gctagtaaaa attccccgat caacaaccac cctgtcagcc
     5701 aggcagcaaa ttcaccaaat acaacataca gataggcata tggcccacca atcactggca
     5761 ctcgcgaagc aaattctgcg aaacttagtc cagacaaaat gacaacgatt gcagctaaga
     5821 caaatgaaag tgacagtgct ggaccagcat tttgattggc cgcaacccct gtcaccacaa
     5881 atattcccgt accgatgatc gcaccaattc caagcatgat caaatcagac gtttttagtt
     5941 cttttttcat ctcactcgta tgattgagat tgatctcttt cttacgaaac aaatccattg
     6001 aagttcctcc ccattttagg ttggaaaaca gcgattaaac tgttttcata taccctaaaa
     6061 ggataccacc taaaatgact aaaaggcaac caacaataac ataaatgagc tcttttttcg
     6121 tttttctttc gccaaggatg aaaatcccac ctaaagtaga gataatgatc cccatttgtg
     6181 aaagtgagaa gccaacagct aaaccaagtg atttgactgt tagaagcatg cagatgtttc
     6241 ctaagcccca taataatcca cagatcatgt ttttccacac aaactgatcg actttaactt
     6301 tacggaaagc aaagaagctt gcgcctaaga tcattccgat actttgaggt aagataattg
     6361 acatcgcatc caaattcgcc cagttgacga tgatggtata tacaccataa ccaatcgtag
     6421 agaagatcaa tgctctaaaa cccttaccaa aatcaagcat tttattatct gtatctattt
     6481 ttccttcgtc atcttgtcgg gcagtcaaat acgcaccgct tacaagaaga gctaaagcaa
     6541 taataccgta tacataatct ttcgtttgtg tccattcatg gaagaaaacg gctcccgcaa
     6601 tcgtattcaa aattaattga aaaccagtag acaatggtag ccctacagaa acacccatgt
     6661 acttcattcc atggaactgt ccattttgtc caacactcca agctaagccg gataaaaatc
     6721 cgatcaagaa aatccaaggg gtgattacag gactaacaac gaaaaagaca actaaagaaa
     6781 acagaaatgc accgatcgtc attcctagtg tttgttgatt ggcatcgcca ccaattttac
     6841 cacttactaa accaatacta ccccatgcca acatagggac taatgctatt aaaatttcca
     6901 taaccaatct ccttttattt cacatgataa gaaacttatc agcgaaaacg ttaacaacac
     6961 aagcactttt tttcgtttct aacgattttc taacgttttt cattataaaa atttaagatt
     7021 gggaaaaata aagtcattgt tgtccctttt ccttcagttg attcagccgt caaatggata
     7081 cctaattttt cggccagatt ttttgctaaa taaagtccta atcctgttga atgtgttttg
     7141 ctaagtcgac catttctccc agtaaatccc ttatcaaaga tccgtttcaa gtcttccgct
     7201 gggataccga tccctgaatc tttaactgaa aggctgactc cttgcgcttc cttttctatg
     7261 ctcacaatga tgtttccgtg atccggcgta tatttgatgg cattactaag caattggttg
     7321 aagataaaag cgatccattt agcatcggtc aaaacagtat gatcctcacc aacgatctcg
     7381 tagcgaatat ttttttggat aaaataattt gcctgactgc gtatcacagg ctgaatgatt
     7441 ccttttagtc catattcttg gatgaggtag tcccgcgaga aactgtcgag tcttgcataa
     7501 tacaaaactt gttctacgta ttcatcgatt tttgataatt cattttctag taatgtatac
     7561 ttttgatcgt caatatcgaa ctcgatcgaa cgaactagca atttagaagc ggaaagtggc
     7621 actttgattt catggaccca tgagtcaata taatcttttt ggtcttgttg attactgatt
     7681 gctgattgca ttacttcttg gtgttcacgg atcagctgat tgatatattc ttctaccagt
     7741 ttttcttctt ctgtcctccc accactcaaa taattttgca aaggggaagt tttttgtatg
     7801 tcggctaatt ttccccacca tcttctcttg ctcaagtaac cacccagcag ataaaaaatc
     7861 agaaagacgc cctcaatcaa agctaaataa gcaattgtgc tcaaatcaac tttgatgttt
     7921 ggcgccagcc acatcacaaa gatcgtcaat gcaataaaaa acagccatcc caagatcaat
     7981 gtccactgat ctttcaaata tttaaaaaaa ttcatcttaa ccgctcatcc tttcagggaa
     8041 cgatatatcc ctgtcctact tttgtttcga tatatccttg gatacctgcc tgttcgattt
     8101 ttcggcgtaa ccgattgatg ttgactgtca atgtattgtc atctacaaat cgttcgtcat
     8161 cccacagtgc tcgcagcaat ttttcccgac ttaagatctt tccattgtgc ttcatcaaaa
     8221 taaataatag acgatattca tttttgctca aatcaatgat ctctccgttg atttccatac
     8281 ttccgttgtc aacattcaac gtaatgccat tgtggctcat catttcacta gataattctg
     8341 tgtagttgta agaacggcga agtaatgcat tgatttttgc aatcagtaca tcgatttgga
     8401 aatgttttgt cacaaaatca tctgccccca tgttcatagc catgatcata tccatattcg
     8461 tattcctgct ggagataaaa atgatcggta ctttcgatac ttcacgtatt ttttgacacc
     8521 aataatagcc atccaaaacg ggaagattaa tatctagtaa tacaagctgt ggttcttctc
     8581 tttcaaagtc tgagaacact tgattgaaat cggtcgttcc aaaggtttca aattgccatt
     8641 tctcaagttc ctccatgatc aattgtcgaa tgatctcttc gtcttcgact accatgattt
     8701 ttgccataaa aaatccctcc atctttcttc ttgctactac aatatcaaag aagcccttgt
     8761 gaaacaagtt ggcttacctg gaaaatgtca ttttagcaaa cgttaaaaaa aactggaaat
     8821 atgcgattta atcgcgtttg tctcttccaa gcagcttaaa tttattatgt tattcttaat
     8881 aattggtcta ttatctgtta tttttctttc gttgtttttg tcttttaaat tttcgataac
     8941 accaataacc gaaaagcgtt agcactagca atatacctag aaggagtata agtaatcgat
     9001 aaagatggta tttttttgtc tgttctatct ttgaagagac ttcttctgta aagggaactc
     9061 gtcttcctgt cactagtaat cggtgagtgt tcacggtgta aggtgtacaa gtgataagtg
     9121 tgactagatc ctgcttttct ctaattgcta atgtatttgt ttttgttgga aggacaacaa
     9181 ttttctcaac gacttcataa gcgagcatcc gaccatacac ttcaagatag aatcggtcat
     9241 cttgctccat ttcttcaaga tccgtaaaga gttttttctc agcaataccg ctatgggcgg
     9301 taatcacgga atgtgtactc tctcctccag ttggaaaaga ggtgccttgc aggactgtcg
     9361 ctcctctatc taacaaaaga tcattcgttt catcaaataa aggcaagcta acattgattt
     9421 tcggaatata gatggctccg atcatatgtt gttcgtaata ttcttttcct ggattttgga
     9481 catttctaac cgcttgctca aaaggatctt tgacttgtcc cattcctggg attactgatg
     9541 tatgagcaag cgtctgattt ttcttttcat gcactgctaa ttgttgctca tgtttttctt
     9601 ctttatcgcg ttcaagctgc ttttgataat tctcgatcct tctttgatcc aaataattgc
     9661 tgagagcatc tgctaaaaat ggatatgtaa aaactgcagc tccggcaaga aacatcactg
     9721 ccataaataa ttgtaataaa agactgattt gggtttgagg tctattacct attttctttt
     9781 tcattttctt caccttctta tttaaaaaaa ctgcagcaac ctttataaaa ccgattgctg
     9841 cagcttattt ttgttgctta tacttcagac ttcattttag atttacggta ccagctgtac
     9901 gctccaatca tcaaacttat tccgattagt aagaatgcca agagcccgtt cccaccagta
     9961 gcaggaagtg acccgcgttt catattcggt actctttctg gccctggatt ttctactcct
    10021 acgatctgaa tttcttcatt atagcttcca taagagatga taaattcagt aaaggctgcc
    10081 tcacctgttg gaagtacata accttctggc gccatggtct ctttcatttg ataggttcca
    10141 taatctaacc cttgaacctc aaagtatcca tttccatctg cgacaaattc tgttgcttgt
    10201 ccgcttgttg cccactcaat tctatctgct cctgattcat aaacagctaa aggcgactcc
    10261 cctttataaa atattgcgta ttcagaacgg tcttcattcc acaattcgaa tcttgcatct
    10321 ttaagcaatt cactgcttga tgcatcatat ttttcaaact ttcttccacc tgttgtgact
    10381 ttctctggcg gctctactcc ttcttcataa tcaaattcat catttcgtcc gatttcaata
    10441 accgctgtgt tgttatggaa atcatccgct tctagttctg tggaaacctt caaattatac
    10501 gtgatcgtta aatctcctcc acgtgcatct gctaatctac ctaaagtatc tactttggct
    10561 actccatttt cttctgtcaa aatcgtcacg acaaacccgt tgcttactac ttctactata
    10621 taatcttgtc cttttgttaa aatcgtctcg ccggctcgca cttctatttc ctgatcataa
    10681 taagttagac caactgtagg cgtatctgtg attttgtaat ttttgataac agcgcttccg
    10741 ttttcaagga cagaatcgat gaaataagga ataggtgctg tgatcgtgta agaagccgtt
    10801 tttcctcttt ctagatcaat ataagaaatc gtttcttgtt ctccagcttc attaataatc
    10861 gttacttgtc taaggtcgct ttcctctaca ttcatctgtt tatctgcttc tctgatttcg
    10921 tttttcggat acaaatgcac atctgtattg tatgtgtctt tccatgtaga accatcccac
    10981 acgccatcca ccatatcagg catcatcact ggcatcataa caaccattgg agacgcgatt
    11041 ctggaaatat gagctggcga ataagtttct aagaaaagat agactttatc tctagaagaa
    11101 ggtgcttctt ccgtcacttg gacggtagag aataccgctt ctccagcttc attcgttgtt
    11161 tgtttgtcaa tacttgtcag tcgatatttt aaaaaccaac tttctgtgtg ccgtttttga
    11221 atccattcaa tcgcttcggc agaattaaga ccgtcttctc gttccggtgt taaaggatta
    11281 tctcgaatca aatcgtaata tacttccgtt acgtcaaaca aatcaaaatc gactcctgga
    11341 atattttctt caccaaaatc cgtagaaacc aatccgctat tcggtcgttc ttccggaaca
    11401 gatgagaaac cttttttgtg taatgtaatc gtcacttcac ttggtttttg tgcgacttct
    11461 tcggctttga ctagttttcc gttcccgaaa atccctattc ccgcagtaac gagtaacagt
    11521 acactcattc ccgtgacaat cattttttta ttcatttttt tctccccgtt ctttttttat
    11581 t
//
LOCUS       DARBEG010000084        10922 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000084 DARBEG010000000
VERSION     DARBEG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.085"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SCK20_002255"
     CDS             complement(<1..281)
                     /locus_tag="SCK20_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146288.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEA"
     gene            423..1541
                     /locus_tag="SCK20_002256"
     CDS             423..1541
                     /locus_tag="SCK20_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1146289.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1669..3045
                     /locus_tag="SCK20_002257"
     CDS             1669..3045
                     /locus_tag="SCK20_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1146290.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3446..5026
                     /locus_tag="SCK20_002258"
     CDS             3446..5026
                     /locus_tag="SCK20_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG1146291.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5224..5447
                     /locus_tag="SCK20_002259"
                     /pseudo
     CDS             5224..5447
                     /locus_tag="SCK20_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5652..5972)
                     /locus_tag="SCK20_002260"
     CDS             complement(5652..5972)
                     /locus_tag="SCK20_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1146292.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6120..8354)
                     /locus_tag="SCK20_002261"
     CDS             complement(6120..8354)
                     /locus_tag="SCK20_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1146293.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8833..9099
                     /locus_tag="SCK20_002262"
     CDS             8833..9099
                     /locus_tag="SCK20_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG1146294.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9099..10826
                     /gene="ptsP"
                     /locus_tag="SCK20_002263"
     CDS             9099..10826
                     /gene="ptsP"
                     /locus_tag="SCK20_002263"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG1146295.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1911 c         2215 g         3317 t
ORIGIN      
        1 gcttcttcaa tcaatatttt tcctaaccct tggtgtctgt attcagaaag aatcgccatc
       61 cgctgtaatt tcatttttcc gttttctaaa ggcaaaagac gtacagttcc ttgcggttct
      121 tttttatctg agtacaggac gaagtggata caatgggctt catctttgtc tatttctcga
      181 gacaaaggca caccctgttc gactacaaaa acttgatgcc tgatccgtac agcatctaag
      241 taaatattgc tcattgtatc ttttgtttgt acaactttca tggtctagcc accttccttt
      301 cgtatagtat actggaaaat aaaacaaata aaaagtatgg caagttttca aaaataaaaa
      361 aagaataaaa gaatttttaa cgaatataat atatactagt aagaaagaaa ggaggaaatc
      421 tattgtttga aaaacttaga cagtctaaac taatgttttg gtcgcttgaa ttattgatca
      481 tagctgcatt gatcatgatt tcatcagaaa taaactttat tttcgaaccg atcgggacat
      541 ttttttccac gttatttgca cctgtactga ttgcaggctt tctttactat ttattgaatc
      601 ctattgtcaa gctgttgatg aaagcaaaaa tcaaaagaat atgggctgtc gcaattgtgc
      661 tgctcctttt agttgcagca attatttgga ttttacttag tgtcatccca agtctagtac
      721 agcaaatttc ctcattggca tctaatatgc ctgatttcat caaacaggta gaaagctggt
      781 taaaagaggt agccgaattg cctttgttca aggaagtaga tatcaataaa tattttgagc
      841 aattagacat ttcttacggg acaatcattc agcaattcct tagtggagta tccaatagtt
      901 taggttcgat cgttggaacg atcgcttcgg caaccgtagt aatcattaca gcaccattca
      961 tcttgttcta tatgttgaaa gacggagaaa aactagtgcc aaacatccaa cagtttttcc
     1021 cagaaaaaag aagaaaccaa attgtggagt tattggggca actgaataaa acattggcta
     1081 attacattag cggacaagca atcgaatgtt tattcgttgg taccttcaca tttttaggat
     1141 atatggtaat tggtgtggat tatgctttct tatttggggt cattgcggga ttgacaaacc
     1201 taattccata tttaggacct tatctaggac tagcacctgc atttctagta acagtcttca
     1261 atgatccggt aaaagcatta ctttgttgtg tggttgtgct aatcgtccaa cagttggatg
     1321 ggaatatcat ttatcctaat gtaattggga aatcactgaa aatccatcca ttaacgatca
     1381 ttatcgttct tctagtagct ggaaatattg ccggattgct tggtatcttc ttaggtgttc
     1441 ctttttacgc aatctgcaag acgatcattt cctatataat caaaatcgtc aaagaagaca
     1501 agcaaaatga aaacaagaag aaaattgcta cgacctcgta ggtagtttta gtacacttgt
     1561 ttttattgca aagaaaattg ctttgtgata ccattttaac gtggctatag tgccacgttt
     1621 ttttctatcg taaactaaaa attaatttta taaggagaga atgcgattat gaatgctgac
     1681 cctgagagtc agtcgctgtt agcgcagatt ttattattga tcgttttgac attgatcaat
     1741 gcttttttag cagcttccga aattgctgtt gtttcgatca ataagaatcg aatcgagcaa
     1801 aaagctgatg aaggagatgt gaaatctcaa aagcttttaa aaatattgca gaatccaaat
     1861 aatttcctat cgacaatcca agtaggaatc actttggtca acattttgtc tggtgcttca
     1921 ctagctaata ctttatcgga acggttagct ccggtattag gtggcggcac agcggcaaaa
     1981 aatattgcaa atatcattgt gctggctatt ttgacctatg tatcgattgt ttttggagaa
     2041 ttgtacccta aacgtattgc tttaaataaa tcagaagaag tcgcaaagtt cacttcaggg
     2101 ctgatacgct tgattggtgt agtagctaag cccttcgtct ggctgctttc tgcttccaca
     2161 agtttattgg cgcgtatcac gccgatgact tttgatgatg aagattcaaa aatgaccaga
     2221 gatgaaatgc gttatatgct tgaaaacgaa ggcgtattga ataatgaaga attagaaatg
     2281 ctccaagggg ttttttctct tgatacgaaa gttgcccgag aagtcatggt cccacgaacg
     2341 gatgcattta tgattgatat caacgacact gtggaagaaa atgtgaatga agttttatcc
     2401 gaaaactatt caagaatccc tgtttataac gaagacaaag ataaagtagt cggaatcctt
     2461 catacaaaga atttactaaa agctgcccat aaattcggct ttgataattt agatatcaag
     2521 aaaatcatgc aggagccttt attcgttcct gaaacggtct ttattgatga tctgttatat
     2581 gaaatgaaaa agacgcagaa tcagatggct attttattag atgaatatgg cggcgtcgtt
     2641 gggttagcaa cactagaaga ccttttagaa gaaatcgtcg gagaaatcga tgatgaatca
     2701 gatgaagttg agaatcttta tgaaaaaatc gatgaacacg aatatatcat ccaaggacgt
     2761 atgctgattg atgagttcaa tgaagctttt gacagtgatc tgcatatgag tgatgtagat
     2821 acgatggctg gttatttgat tacagcatta ggcatgattc cggatgaagg cgaaaagtta
     2881 tcgtttgatg tcgataatat tacgctggtt tctgaagaaa tggaaggttc tcgaatcctt
     2941 aaaatccgtg tcatttttca tgatccggaa gaaacagaag cggaacctga agaagaacga
     3001 agatacttca gaaaagaatt cgaagatgac gagccaagaa gataattgga aacttggtta
     3061 tgaaagacgt gttgcttgaa taaacaagca acacgtcttt tcttttagtc agctgacaaa
     3121 aaaatatttg actcttatgg tttggtggct ttttcattcg ctgaatccat gctatactga
     3181 ttcagttgta gtagttgaca aaggttgtga aaaagctatc ctgcattaag taataagagc
     3241 agccaagcaa aaacagcttt tcgtattttt ggcttgggtg ggcttattgc tgataggctg
     3301 tctctagaac gccgtttact cggaaaaaga gactgctaca cggcttttgt cacagcccta
     3361 tatgaaattg tctttcttta agagatcgga caatctgtgc ctagagaaaa tataaataat
     3421 tgaatggaag aaatggagtt aggttatgaa taatccaaaa ttaaagcaac aagtggacaa
     3481 tcgcagaact tttgcgatca tttcccatcc ggatgctggg aaaacaacga ttacagaaca
     3541 attactattg tttggtgggg ctatccgtca agccgggacc gtaaaaggca aaaaaacagg
     3601 taattttgcc aaatcagact ggatggaaat cgagaaacaa cgtgggattt ctgttactag
     3661 ttctgttatg cagtttgatt atcaagggaa acgagtaaat atcttggata caccaggaca
     3721 tgaagatttt tctgaagata cttatcgtac tttgatggcc gtggatagtg cggtcatggt
     3781 aatcgatagt gcaaaaggga tcgaagcaca gacaaagaaa ttattccaag ttgtaaaaaa
     3841 acgtggcatc ccgatattta cttttatcaa caaactagat cgtgatggtc gtgaaccatt
     3901 ggaattgtta gaagaattag aagagctatt agatatcgaa tcttatccga tgaattggcc
     3961 aatcggtatg ggtaaagggc tagaaggtct ttatgatatc taccataatc gtgtagagtt
     4021 ttatcgtccg gaaaattatc aggatgaacg attagtagaa ttggatgaag atggactgct
     4081 tcccgaaaat catcctctaa ctgaaaattc cttatatgaa caagtcttag aagaagtaga
     4141 actaatcaaa gaggctggag atgcatttaa tcaagaaaaa attgctcgtg gcgatcagac
     4201 accagtattt tttggttctg cattgactaa cttcggtgta cagacttttt tagagacttt
     4261 cgttgaattg gcaccagctc cttatggaca caaaacacaa gatgatcaga tggtaagtcc
     4321 ttatgaggaa gaattttcag gtttcgtatt caagatccaa gcaaatatga atccagccca
     4381 tcgtgaccga atcgcttttg tccgaatctg ttctggaaca ttcgaacgag gaatggatgt
     4441 ctggttggaa cgtacaaata aaaaactgaa attaagcaat gtcacacaat ttatggcaga
     4501 ttcgagagaa aacgtcgaaa aagcagtagc aggcgatatc atcggtgtat atgatacagg
     4561 caattatcag attggggata cattgttcga aggaaaacta aaagttgctt atgaagaact
     4621 cccttcattt actccagagc tgtttatgaa agtgactgca aaaaatgtca tgaaacaaaa
     4681 atccttccac aaaggaatct atcaattagt tcaagaagga gcgattcagc tttataaaac
     4741 atatctaacg gaagaatata tcattggagc tgttggtcag ctacaattcg aagtattcca
     4801 atatcgtatg ttaaatgagt ataacgccga agtaatcatg tctccaatgg gacacaaaat
     4861 cgctcgctgg atcgatccag aagatttgga tgagaagatg agttcaagtc gcaatatctt
     4921 ggctcgtgac cgtttcgacc aaccgttgtt cttattcgag aaccaatttg cagaacgctg
     4981 gtttgcggat aaatatccca atgtaaaatt aaaaagtttg atgtaaagaa gtcttgacac
     5041 aagctgccct gcatcaagta ataagaatag ccaaacaaaa acagcctctc atattttgtt
     5101 tagagccgtg acggattttc cgtgacggct ctttttacta tattaaaatc atagaaatgt
     5161 ctaatcaaaa aaatataaaa attattttgc gtacatatga ttaaagaaag gagagtgtga
     5221 cacatggaaa cagaatggca aagacgtgaa agaaaaaaac agaaaaagaa gagatgttac
     5281 gattatgtcg attattagat gtaaaagact gggacaaaaa cgagcaaatt gcacaaaaag
     5341 tacgatcaat catccataaa gaggaaaaag ttaaaaataa agacacgcgt agaactgaaa
     5401 atgaaaagag aaaagtacaa aagaaaagag aaaaatcaaa aagctagaaa gaaaaagagg
     5461 ctgagacaga agcgtttaac tccaagaaat aagctggaat tcacaaaaat ttgaaaagca
     5521 attttcgtga atttcagctt atttccgaag gagttgcttc tgctttcgcc gtttatacgt
     5581 gtttagagcg tgagacaaaa gtgttcttta cttttgtccc acgctcttta attattatgt
     5641 tcaaaatttg tttacacggc taatgtagcg cctaattcat gttttggaat cgagatttca
     5701 acaaggcctg ccaattcgat tactttgatt tcatcctcgt ggtaaacggc gtctttgatt
     5761 ttatctaaga aataattttt agcatcttcg atttctttcg gctggatttt acgtttgtca
     5821 ttgatcagca gaatttcatt atgttcaggg gcttcagtgt agacaatagt ggtatttccg
     5881 gctgtgtaga ccttgactag atgggcatca gtgttttcaa gctggtttaa tacaagacgg
     5941 gaatggctgt ttgttacatt taccaatttc atggcgctca cctcttttat gtatttttaa
     6001 ttgcttgttt ctcttattat aatttctttt ttataaaatg aacagtattt tgacttttct
     6061 gaaaaaagaa tgagacggca tttctcaaag aaataccgtc tcacgctata aaactggatt
     6121 tattcagccg tttcttcggc tgattcttta gccaaacgtt ctggtttaga agtaacgatg
     6181 atttttccat ctttatccag ttttgctttc aactcatgga tagaagggtg atcaagataa
     6241 aattcagcga taccatcttc gatttgttct tggatcgtgc gtcgaagagg acgagcaccc
     6301 atagatggat catagcctaa gtcaactaat ttttctttga cattcgtagg aacttcgatg
     6361 tgaagttgct gtgctgccag catttggttg acatcgtcta gcattagtgt gacgattgtc
     6421 atcaagtttt cttttgataa tgcactgaat tcgatgatcc cgtcaaaacg gttcagaaat
     6481 tctggtgtaa agtagttgtt taattggttc aatacagaac gagtaacacc ttcgcgtgcc
     6541 gcaccaaaac caacgtttgc ttctacttta ccagttccgg cattacttgt cataatgatg
     6601 atcgtatctt taaagctgac cgttcttcct tgtgcatctg tcaatcgacc atcatctaag
     6661 atttgcaaga acatatgcaa aacatctgga tgagcttttt ctacttcatc taacaagaca
     6721 agactgtaag gatttctccg tactttttct gttaattgac cagcttcttc gtatccgaca
     6781 taacctggag gagaaccaat caatttagag acactatgtt tttccatata ttcagacata
     6841 tcgaaacgaa tcattgaatc ttgtgaaccg aatagttcgt aggcaagttg tttcgcaagc
     6901 tctgttttac ctacaccagt tggtccaaca aataagaagg agccgattgg acgattctgt
     6961 ttgtttaatc caacacggtt tcggcgaatc gctttcgcta ctcgatctac agcgttatct
     7021 tgtccgatga catgagcttt caagtcatct gctaagtttt tcagttgtgt ctgttctttt
     7081 tcttttaatt cacccactgg aatacctgtc cgttgttcaa cgattttttc catatctttt
     7141 tcggtaatca caggtgtttc ctcttcagtg agctgacgtt ctttcatttt ttgaagttta
     7201 ttgatctgat cgcgataata agctgctttt tcgaaatctt cttctcgaga agctaaaact
     7261 ttttgttctt ctgcttcttg cagttttttc tcgattgttt taggatcaac gatttggatc
     7321 gtcaagtttt tcttagaacc tgtttcatct agcaaatcga tggctttatc cggcaagaag
     7381 cgatcttgga cgtaacgatt ggataaagtt gcggctgctt cgatggcctc atcagtatat
     7441 tttacatgat gatagtcttc ataacgtttt tgcaaacctt tcaaaatact gatcgtttct
     7501 tcgacagttg gttcatctac ccgtacaggt tgcatacgac gttctaaagc agcgtctttt
     7561 tcgatgatgc ggtactcatt taaggtagta gcaccaacca tttgcaattc tcctcgagct
     7621 aaagctggtt ttaaaatatt accagcatcc atatttccat caccagcaga accagcaccg
     7681 acgatttcgt gaacttcatc tatgaatagg atcacatttt ctgcttgacg gatctcttca
     7741 atcaattttt gcatacgttc ttcaaattgt cctcgtatac cagtaccttg gactaaagat
     7801 acgacatcta aacgaatgac ttctttgtcc attaattttt gcggtacatc accatctacg
     7861 attttttgtg ctaaaccttc aacaactgct gttttaccaa cacctggttc tccaatcaag
     7921 acaggattgt tcttggttcg gcgatttaaa atttcaatga ctcgtttgat ttcgtcatcg
     7981 cgtccgatca caggatcgat atcaccttgt cgtgcttgtt ctgtgatatt gattccgtat
     8041 tctcccaata aaccttctgt attgccttga ggtggctgac caccattgaa gttgttgttt
     8101 ccgccaaatt gtgttggtgg tgtttgctca taatttcctt gctgttgcat ttggcgagac
     8161 atggaacgga aaagatcgtc caaactgcca aatccaaatg gatcattttg ggccatatta
     8221 gtcagacctc cattttgttg atttttgatt ttttgataac agctctgaca gtaatcgagt
     8281 tgtgtccgct gaccattgac agtggcatac aagtgaatcg ttgcctcatt cttgccgcag
     8341 ttttgacaaa gcatgaatca ttcacgtcct ttcacaaaaa tgggtacatc catattttac
     8401 atctcgtttt tcatgaggta aaagaataac ccctagtttt ttaagagatt tatgaggtag
     8461 aaaaggtgaa atctctattg accattcctg acctttgact cttattatac taaaaataaa
     8521 aagggaagca agtaattcgt ttcatatgaa aacaaagagg gtcaaaggaa agaatgataa
     8581 acgtcaggag aagagaaagc gacaagaaaa aggaaataaa cacgtttgtt ataaaatgtg
     8641 aagaaaagga tgaaaaagaa tccggaataa tttgaatact tttatctgaa attgaatgaa
     8701 aactgttgta tctaacgaaa aaaaatgtta atctttacag atgaaagggt ggcatttgtt
     8761 aggattctct gaattactaa aaaagaaaac cctcacataa aaacaacact cacttaaagg
     8821 agaccgatta atatggaaaa gaaagaattt cacgtagtag cagaaactgg gatccacgct
     8881 cgtccagcta cactattagt acaaacagca agcaaattta actctgatgt aaacttagag
     8941 tataaaggta aatcagtaaa cttgaaatca atcatgggcg ttatgtcttt aggtgtaggt
     9001 caaggttctg atgttactat tactgcagaa ggtgctgacg aagctgatgc aatggcagca
     9061 atcgttgaaa caatgaagaa agaaggctta tctgaataat ggttgaaatg ctaaaaggga
     9121 ttgccgctag tgacggtgta gccgttgcaa aagcttacct gctagttcaa ccggatttaa
     9181 cattcagtaa ggcaacagta gaagatactg cggctgaaga agctcgtttg gatgctgcat
     9241 tagctaaatc tactgaagaa ttgcagcaaa ttcgtgaaaa agcagcgcaa agcttaggtg
     9301 aagcagaagc gcaagtattt gatgcccatt tgatggttct ttcagatcca gaaatggtag
     9361 gtcaaatcaa gcaaaatatt aaagacaaca gcgtaaatgc tgaatcagcg ctaaaagaag
     9421 taacggatat gtatatcggt atgttcgaag caatggaaga taatgcttat atgcaagagc
     9481 gtgctgcaga tattcgtgac gttgctaaac gtattttagc gcacttatta ggcgttactt
     9541 taccaaatcc atcaatgatc aacgaagaag tggtcgttgt tgcacatgac ttgactccaa
     9601 gtgatacagc acaattagat cgcaattttg taaaagcgtt tgtaacagac attggtggac
     9661 gtacttcaca ttctgctatc atggcacgtt ctttagaaat tccagcaatc gttgggacaa
     9721 aagaaattac tgctaaagtc aaagaaggcg atattttggc tgtcaacggg atcgaaggcg
     9781 atgtaatcat cgatcctaca gatgaacaaa aagctgaatt tgaaaaagca ggtgcagatt
     9841 acgcagcaca aaaagcagaa tgggaaaaat taaaaaatgc agaaactgtc acagctgacg
     9901 gcaagcattt tgaattagct gcaaacatcg gaacaccaaa agatctagtt ggtgtccata
     9961 ataatggtgg agaagcagtt ggtctgtacc gtactgaatt cctgtatatg gattctccag
    10021 acttcccaac agaagatgac caatatgaag cttacaaagc tgtattagaa ggtatggaag
    10081 gaaaacctgt agttgttcgt acaatggaca tcggtggaga taaagaacta ccatatctgc
    10141 aattacctca tgaaatgaat ccattcttag gatatcgtgc attgcgtatc agtttatctg
    10201 aacaaggcga cgaaatgttc cgtacacaaa tgcgtgcatt gcttcgtgca tctgttcatg
    10261 gtaatttacg tatcatgttc cctatggtag ctacactcaa agaattccgc gcagcaaaag
    10321 cgatctttga agaagaaaaa caaaaattga taagtgaagg caaggaagta tctgatacga
    10381 tccaagtcgg catcatgatc gagattccag cagcagcagt cttagcagac aagtttgcaa
    10441 aagaagtaga tttcttctct gtaggtacga acgatttgat tcaatacaca atggcagcag
    10501 accgtatgaa cgaacgcgtt tcttacttgt accaaccata taacccatca attttacgtt
    10561 tgatcaaaaa tgtaattgat gcagcacacg ctgaaggaaa atgggcaggt atgtgtggag
    10621 aaatggctgg agatcaaaca gctgttccat tgctagtagg tatgggtcta gatgagttct
    10681 caatgagtgc gacatctatt cttaaaacgc gtagtttaat gaaacgcttg gacacagcga
    10741 aaatggctga acttgctgat cgtgcattga aagaatgcga tatgatggaa gaagtcgttg
    10801 aacttgttca tgaatatgta aaataaacaa cgtatcaaaa gctggtgtgg cttgtaaaag
    10861 agctacgcca gtttttttgt tcttttttaa gaaaacgttt ttgtcagtaa gaatagacaa
    10921 aa
//
LOCUS       DARBEG010000085        10817 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000085 DARBEG010000000
VERSION     DARBEG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.086"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SCK20_002264"
     CDS             complement(25..162)
                     /locus_tag="SCK20_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1146296.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SCK20_002265"
     CDS             complement(159..1343)
                     /locus_tag="SCK20_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG1146297.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SCK20_002266"
     CDS             complement(1599..1853)
                     /locus_tag="SCK20_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146298.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SCK20_002267"
     CDS             complement(1878..3020)
                     /locus_tag="SCK20_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG1146299.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SCK20_002268"
     CDS             complement(3080..4432)
                     /locus_tag="SCK20_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1146300.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SCK20_002269"
     CDS             complement(4503..4847)
                     /locus_tag="SCK20_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1146301.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SCK20_002270"
     CDS             complement(4857..5231)
                     /locus_tag="SCK20_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1146302.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SCK20_002271"
     CDS             complement(5244..5558)
                     /locus_tag="SCK20_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1146303.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCK20_002272"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCK20_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SCK20_002273"
     CDS             complement(8976..9638)
                     /locus_tag="SCK20_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146304.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="SCK20_002274"
     CDS             10255..10485
                     /locus_tag="SCK20_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146305.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="SCK20_002275"
     CDS             10491..10790
                     /locus_tag="SCK20_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146306.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DARBEG010000086        10724 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000086 DARBEG010000000
VERSION     DARBEG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.087"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(241..1089)
                     /locus_tag="SCK20_002276"
     CDS             complement(241..1089)
                     /locus_tag="SCK20_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1146307.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1088..2155
                     /gene="gnd"
                     /locus_tag="SCK20_002277"
     CDS             1088..2155
                     /gene="gnd"
                     /locus_tag="SCK20_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG1146308.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2157..3158
                     /locus_tag="SCK20_002278"
     CDS             2157..3158
                     /locus_tag="SCK20_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG1146309.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3155..3439
                     /locus_tag="SCK20_002279"
     CDS             3155..3439
                     /locus_tag="SCK20_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG1146310.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3472..4269
                     /locus_tag="SCK20_002280"
     CDS             3472..4269
                     /locus_tag="SCK20_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1146311.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4283..5116
                     /locus_tag="SCK20_002281"
     CDS             4283..5116
                     /locus_tag="SCK20_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1146312.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5134..5991
                     /locus_tag="SCK20_002282"
     CDS             5134..5991
                     /locus_tag="SCK20_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1146313.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6013..6522
                     /locus_tag="SCK20_002283"
     CDS             6013..6522
                     /locus_tag="SCK20_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1146314.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6559..6960
                     /locus_tag="SCK20_002284"
     CDS             6559..6960
                     /locus_tag="SCK20_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146315.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7072..7425
                     /locus_tag="SCK20_002285"
     CDS             7072..7425
                     /locus_tag="SCK20_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146316.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7459..7902
                     /locus_tag="SCK20_002286"
     CDS             7459..7902
                     /locus_tag="SCK20_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1146317.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7919..9004
                     /gene="uxuA"
                     /locus_tag="SCK20_002287"
     CDS             7919..9004
                     /gene="uxuA"
                     /locus_tag="SCK20_002287"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1146318.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8997..9710
                     /locus_tag="SCK20_002288"
     CDS             8997..9710
                     /locus_tag="SCK20_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1146319.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9917..10066)
                     /locus_tag="SCK20_002289"
     CDS             complement(9917..10066)
                     /locus_tag="SCK20_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146320.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10462..10662
                     /locus_tag="SCK20_002290"
     CDS             10462..10662
                     /locus_tag="SCK20_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1146321.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3588 a         1695 c         2320 g         3121 t
ORIGIN      
        1 aaaaataata aaaaaaagaa aagataaatt ttttactact gtatactaac tttgttatcg
       61 catctaacaa gtaactcgaa taataccaat acaaccttct ccaaagatgc gatgacatac
      121 gttctaaccc accgactatt gatgttctgt accacccttg cagaaccaaa aaagccacta
      181 ccaaatattt ggtagtggct ttttttgttt tatggatttt tcgctttttc tgttatggtg
      241 ctattctttg atttcagaaa tagtatgtcg aactttagcg atagaacgac gtgattcttt
      301 ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa atgcttagtt gagaaatacg
      361 ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa atagaaataa aaacgacatc
      421 gccaagttta gcaagggaag aattagcttg gctagtaata acgattgttt ttgctccagt
      481 ttttcttacg gtttcagcaa tatggattgt ttcttttgat tgccctgtgt gagaaatcaa
      541 aacagcacag tctttttccg tcaataaaga agcttccatc aattgaatat ggtaatccgt
      601 gctgtgagaa gttgcgatag gggaacgcaa aaatttatga taagcgtcag ttgctacaat
      661 ctctgaaccg ccaacaccga aaaaatagac acgtttagat tcattgataa tagtagccgc
      721 tttttttaga tccttttgtt gtaacaactt ttttgtgtca tttaaagttg aaatactaga
      781 ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt ttctgaatat tttcgtggat
      841 agaaatagca gatagatcat tttcttggat caacatggcc attttaaaat ctttaaatcc
      901 attataacca agtttttttg taaattgaaa aaaagtagag tcagcaatgc ccagttcact
      961 tgctaaattg ctaataggac tatgacttac tttgcttgga ttttctaaaa tataatcggc
     1021 gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt ttgatttgta gtcttatgga
     1081 gacgctcatg agaattcctc attttcataa taagattatc attttttccg ttctcattaa
     1141 tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa aatatttttg taaaatctat
     1201 ttacaaaaaa atttttttgt ataatgaggg tataagaaat ggaggcgcta tcatggaaat
     1261 tggtgtaatt ggtttgggaa aaatgggatt gaatttagct ttaaacttaa aagagcacca
     1321 tcacaaagta caaggactcg acatttcaag tacagctgtt agtcaggcaa gagaaaatga
     1381 cattcctgct tatcaagaga tctctgaatt tctagctact tttgagcaga aaaaaatcat
     1441 ctggttaatg cttcctgctg gagagataac cgaaaacatg ttaagaaaac tttttccaaa
     1501 actcggacca ggagacatta ttattgaagg cggaaactct tattataaag attcaatccg
     1561 tcgagcagaa gagtttcaga aaaatgaaat cggttacttg gattgtggga cttctggagg
     1621 aatcgaagga gcacgacatc aagcctgctt aatgattgga ggagataaag aaacttttct
     1681 atccgtggaa caattattca aagatgtagc aattgaaaga ggatatctgt atgctggtgc
     1741 atccggaagc ggacacttct tgaaaatgat ccataatgga atcgaatatg gaatgatgca
     1801 agcaatcggt gaaggattcc aacttgtaca aaaaagtgag tatgcttttg acttggaaca
     1861 agtcgcacgt gtttggaatc atggttcggt gattcgcggc tggctgatgg aaatcatgga
     1921 agatcagtta aacgaacatc ccaatttaag taattatcgg ggaatcgttt ctgcatcagg
     1981 tgaagcaaaa tggacggttg aaacagcgct agaaatggct gttccagttc cagtaattgc
     2041 aactagtttg ttcatgagga atctttcaca ggaagaagat agtttttcag caaaagtagt
     2101 ttcagcacta agaaatgggt ttggcggaca tgaaatagtt gaaaagggtg agtaaaatgc
     2161 cagattatca tatagcaatc gttaattcca gcagtttcgg aagagtcttt ccacaccact
     2221 tggaaagatt aaagtcagtt ggagacgttc atttctttac tgttgatcaa gagattccag
     2281 gaaaagaact agcggagtta cttaaaggat tcaatatcat tattgcaagt gttacgccat
     2341 tttttcgacg agattttttt gaaaacaaag atgaactgct attgatttcc cgacatggta
     2401 ttggttacaa caatattgat ttaaaagcag caaaagaaca ccagacaatc gtatctatca
     2461 tacctgcttt agttgaacga aatgctgtag cagaaaataa tataactaat ttgttgtcca
     2521 tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga cgatcgatgg gaagatcggg
     2581 cggagttcgt cggtaggacg ctgtttaaca aaacagttgg tgtgattggt gcaggaaata
     2641 ccggaagcgg agtaatcgaa attttgcgta acggttttcg ctgtgatgtt ctagcttatg
     2701 atccttacaa atcagattta tacatcgaaa gctttggtgc aaaaaaagta gaacttcaag
     2761 aattactgga acaatcagat atcatttgtt tatgtgcgaa tttgacagaa gagaactatc
     2821 acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa tgtttatcta tctaatagtg
     2881 caagaggcgc tctagttgat gaagaagcga tgattaatgg tctgaaaagc ggaaagatcg
     2941 caggatatgc cacagatgtt ttagaagaag aaccgggacg tcaagatcat ccgtatttag
     3001 cgtttgaaca agtagtgatt acgccacata tatcggccta cacaagggaa tgtttagaag
     3061 caatgggtga aaaatgtgtc agagatgtcg aacagatcag tcagcacctt cttcctgaac
     3121 gttctgtcca gacaaagagc aactatataa aaggatgatg aagaaaatgg tgaacggttt
     3181 aaaagtgaaa acaggccctc aattttattt atatgaagaa ggtggtattt caaaagtttc
     3241 tgatctcttg aaatcatacg gcgcgaaaag agtactggtc acccacggaa cggtatcatg
     3301 ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa acgattcaat ttttttatca
     3361 tcgttattct ggagaatgta gttatgctga agccagacga atagctacaa tcatcaaaaa
     3421 aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac tgaccgatct agtgggttat
     3481 agtgccaatc ttgcaagaat cgatttcgga gtcatcccta ctttagctag taattgtgct
     3541 ccatggacac ctcttgctgt catgtataag gagaatggac aagcagaagg gaaaacagaa
     3601 cacttttaca gacaagctgc tttttttata acagatcccc aattagtgat tgattcccct
     3661 gttccatatt tcattgccgg gttagcagat acactagcaa aatggtatga atctgagctc
     3721 atcttagaac aagatttttt gcaagatgaa tcatttttga agctagcaca agatactgcg
     3781 aagatctgca aagaagagat tttgaatcat tctgctaaag caatccaaga tatggcacaa
     3841 agaaaactga catcggagtt caaacagctt tcagagatta tctttgctgt tgctggttta
     3901 gttggaggat taggagataa atacgcaaga aatgcggcag cacatgctat gcatgacgct
     3961 atcagtaaat atattccgga aagccatcgt tttctacatg gagaaaaagt agcgtatggg
     4021 attttctatc aactggcact agaaggtcgc tgggaaaaaa tagacgagtt atatcctttg
     4081 tatcgggaat tagaactgcc ggtttctcta gaacaaatgg gattatttcc gaaagataaa
     4141 cagataatag ataaaatggc tgaatttatt gattcgaaag agaaagtgca tttgattcct
     4201 atagaggtaa cacagaaatc actgatacag tcaatgatgg aactggaagg ttatatcaga
     4261 aagatctgaa ggaggaggac acatggaaac attaagtgta gtacaaagtt tgttgattgc
     4321 tttatgggta gccgcggtta tgtcccgctg gttaggtgga ggagccacgt tgaccttacg
     4381 tttttctcct ttgatgactg gattagtcgt cgggttgatc atgggggaag ttgcacaagc
     4441 gatgattgtt acagccgctt tgcaaatgat ttatatgggg ggtttttctc caggaggatc
     4501 aatgccagct gaaccatcta ttgcagcagc tatcgcagta ccagtcgcat tattagggga
     4561 tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc ggattgctcg gaagttactt
     4621 gtatcagttt cgttttttca tcaatacttt tttaggtaaa tatacagaca aagctgtaga
     4681 agatttgaat acaaaagcta tggcccgatc aattattctt tatccaacgg ttgcctcatt
     4741 tttattattc gttccgcttg ttttcattgc tttatattta ggggcgccat tgatcgctga
     4801 tgtaatcaaa gctttagagg gaacagtcgt tatccacatt cttgaagtag taggtggagg
     4861 gttagcggca atcggtatcg cgacaactgt atacgttatc ggtcgaaaag attatctggt
     4921 ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa tctttagata ttacgatggt
     4981 tacttatgcc atttttggga ttattatcgc tttgatcttt gtccagtcgc aaaaagggaa
     5041 acaagtacag cctgccacaa gcaataacaa ttcagcatct tttgatgacg aagatgacta
     5101 tgatgacgga ttttaattta ggaggaatgg gaaatggctg aaataatgga taatcaaaca
     5161 ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag cctatctgag atggcatttt
     5221 gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc catcactact atacggaatg
     5281 atgccaatct tgaaaaaact ttataaagat gaagactctt taaaagcagc atataaacgg
     5341 cagttactgt tcttcaacac tcaattaagc tggggtggcg gtgtgattac tggcctaatg
     5401 tcttctatgg aacagcaaag agcagaagaa gagtatgaag aaaaagagat cttgatgcag
     5461 gatgacttga tgtataacac gaaagcggga ttgatggggg cactagcagg aatcggagat
     5521 gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa tcgcggtacc ttgggcacaa
     5581 cagggaagtc ctttaggcgc actttttcct tttatctgct ttgggttgta tcaggtttta
     5641 ctaggggtat ttttcgcaag acaagctttc actatgggcc gtaatgctac aggcctaatg
     5701 caaaacactg gtgttcagac agcgattgaa atgctatcag ttttagggtt gtttatgatg
     5761 ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat tgaaatttgc gatatccgga
     5821 cgtgagtttg ttatccagga aattcttgat caaatcgtgc cagggatatt acctttagct
     5881 gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat tgaaagtcac acaagcatta
     5941 ttatggctga ctggaatatt aattgtgcta gcaggaatcg gtattttata aaaagaaggg
     6001 aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg atgagcgttt gatccatggg
     6061 caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt ccatttttgt tgtagacgaa
     6121 gttgtagcaa aagataaatt tatgagagat ctttacaaaa atgcaggaag ccgtacagga
     6181 caaaaaacaa ttgtcattac accagagaaa gctaaattct attgggatga atatcaattt
     6241 aaagaataca attgtatttt gatcgcaaaa acagtccaaa cgatttacga cttagtaaaa
     6301 cacggaatcc ccatggaaga attgaatatt ggagggattg ctcaaaaaaa tcctgataca
     6361 gatcttttag tgacgaaatc tgtttattta aataaatcag atgctgaaaa attgaaagaa
     6421 atgaatgaac aatatggcgt aaagaatatt tattttcaag ccactccatc tgcacctaaa
     6481 acaggattaa aagatgtatt gaacaagttt gatttgtcat aaacaaatgc ttagaaaaaa
     6541 gataaagagg gcgaaaaaat gaagaagaac gagctgtttc gtgattggga atttcgttat
     6601 cgctatattt accgtaagcg ccgtacaaaa aagagtaaac agcgttttct ttcagcatta
     6661 gtatcagata tttacagtat gagaacagat gtgacagtca ttgcttacga tacgctagcc
     6721 taccgatcaa aaaatattta tgttggagat atcgaaaaag cagagaaagt tatctgtact
     6781 tattacgata cacccgttca tgcattagga agttatttca tgtttgattg gaaagaccaa
     6841 aggaaaaaaa caatctactc tatattattg tccttcattt tgttgttcag tttaggttgg
     6901 tggggaatga tgatatataa caaaaatcct catcacgtat tcgatctgct atccgtataa
     6961 acaggtatca ctgtactagc atttgggagc tactttttct tattgggaaa agcagctcgc
     7021 ggatggccta gcagacaaac ttttattcga aatacttcat ccatattaac gatgctagaa
     7081 atgatccgta cgattgatga tccgagtgta gcgtatgcgt ttgttgatga aggctgttat
     7141 ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa aagaggctat tcttttttat
     7201 ctagactctg ttggggcaga tacaccgcta cagttttcgg gaaattattt ttcaaataaa
     7261 gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa acgtcaatta tatttttagt
     7321 gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa cagatttacg agggaaaaca
     7381 tttaattggc aaaatgccaa tcagattatt gctctttttc gataagtaaa aagtgcaaaa
     7441 caaggagtga gaagactaat gttggaaatc atgatcgtta cacatggtaa gttgagcgaa
     7501 ggactgaaag atgcggcgga agtaattgtc gggacatcga gccatattga aacaatgtgc
     7561 ttagagtctg gggaggacat ccaaacattc ggagagaagg tcaaagaaaa attaactatg
     7621 atcaacacag cagaacccat gctagtattt gttgatctaa ttagcgcaag tccgtacaat
     7681 caagcgctct tggcagtcaa tgaactgact gaagaaaaga aagcagccac ttatgtcata
     7741 gggggcgtga atctgccaat gttgttagaa gcaatcaatc atcagatgct tcacacaccg
     7801 atagaagaag cggtggaaca aattgtcaaa caaggaaaag ataatttaac tgtttggcat
     7861 gcttcagcgg aagaaaaaaa attagatgag gacgattttt agaaggaagg ctgaaaagat
     7921 gaagtgggga tttagatggt atggtgaaaa aggggattcg atcccattaa aatatgttcg
     7981 ccaaataccg ggaataaatg ggataattgg aactttgctg actaaaatgc ctggagatgt
     8041 ttgggagatt tcagaaattg aggagttgaa agcatctgtt gagagccaag gactggaact
     8101 gcttggaatt gaaagtgtag cgatccacga tgcaatcaag gcagggacag atgaacgaga
     8161 ttactatatt gataactata tacgaacaat caaaaacttg tctcgatgtg gggttcgatt
     8221 gatttgttat agcttcaaac caatttttgg atgggcgaaa actgatttgt ttaatgaaaa
     8281 caacgatgga agctattctc ttttatacga tcagaaaatc gtcgatcaga tggagccagg
     8341 agaaatgtat caattgatac acagccagtc aaagggattc aaattgccgg gttgggaaga
     8401 agaacggttg aaaaaattcg ctcagctgga aaaactgtat gaaggaatga ccgaagaaga
     8461 attatttgaa aatctgactt attttttaga aaaagtgatt ccagtgtgcg aaaaagcaga
     8521 tgtaaaaatg gctatccatc cagatgatcc gccatgggaa ctttttggtt ttcctagagt
     8581 gacgaaaaat cttggcgact tgaagaaaat tctaaatgca gtagattctc catataatgg
     8641 actaacgctg tgcacaggat ctttaggggc aaatccggaa aacgatttag ttgaaatcat
     8701 ccatgaggta ggttcgcgca tccattttgt ccacttcaga aacgtggagt ttttaggaga
     8761 aagaaagttc aaagaaacgg cacatccctc tacagaaggc tcccttgata tgtatcatat
     8821 tatgaaagcg ttggtgcaag aagggttcga cggtcctata cgccctgatc atggtagaac
     8881 cgtgtggggt gaagtggcta tgcctggata tggcctgtat gatcgtgcaa tgggactcac
     8941 ttacatgcaa ggtctatatg aggcgataag taaggaaaaa ccactgggag agcattatga
     9001 ataaaatcaa gctcttcatc tttgatatgg atggcctttt attagaaact ggacgtttag
     9061 cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta tgaaatgcgt aaggaagtct
     9121 attatttact aactggccaa acagaaatgg cgataagaga acaaatgggc attttgtacg
     9181 gagaagatgt tccatatatc aaatggcgag aagcaatcaa tcaatataaa gaaaaaatcg
     9241 tgaaagaaga taagagagta tatacgaaaa aaggagcaga agaaatttta tcttttgcaa
     9301 aagaaagagg aatccataca atcgtggctt cgtctaatac acgggtaaaa gtagaaatgt
     9361 atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat catttcaggg gatgatgtaa
     9421 aaaaaggaaa gccggaacca gagatctttt tgaaagcttg ttcaaaaatg aatattcctc
     9481 caagtgaagc acttgtattt gaagactcga ttgcagggat cgaagcagca aggcgagcgg
     9541 gcattttgtc ttttctcatc aaagatcatt tgatagggtt gcccgatcat aatgggaagc
     9601 ataagttgaa agtagatgta agtttatatg aatccaataa agcagactat gaattttatg
     9661 acctgcatca agcaaaacga tttttagaaa gtaaagggtt gtatctctaa taaagaggac
     9721 tattttctag tcctctttat ttattgtcct gcacgactga gggaacagat gaaaaagaag
     9781 taatttttta ttttgaagta tctaccttat ttgtagtgaa caatttagaa caaacacaaa
     9841 acgcatctac tgttatctac cagtagatgc gtttaaagag aagaacgaga atcgtccata
     9901 tgagggtttc cactccttat gcagattttt ttaattcttg cactgcttgt tcgatcgtaa
     9961 cacctgtcgc tacatgattg gtattttctt tgtcaaaaac gacaaataga ttcaaatctg
    10021 tatccaaggt tactcgatat tgtatatctt tgttatagaa tgtcatagcc attctccttt
    10081 ctttaaatac ttgctgaaag gcaacgaacg cgttgataac gaaattccat ttataagtat
    10141 accaccaatt gaaaggtttt catagttaag aaattttaaa gtcaaaagtt gcagataaaa
    10201 gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt attcaacttt ttcttcgatt
    10261 cgaagacaat agaagacaat gaattttata tttcattgca ttatccttgt gttattagaa
    10321 aaagcatgat atattaaatg agtaatctat tttgaaacgt aatttgagcg atatatctac
    10381 atagataaaa ctccttttgc ctatcaaata tttattgcaa cgaaaaaacg tacattgata
    10441 cgtatcagga ggaatttatt tatgaataac ggtacagtaa aatggttcaa cgcagacaaa
    10501 ggttttggct ttatcactgg tgaagatgga aatgatgtat ttgcacattt ttcagctatc
    10561 caaggtgacg gcttcaaaac tttagaagaa ggccaagcag tgtcttacga tgttgaagat
    10621 ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt aataaaaatt gacttttaac
    10681 cgtcttttcg aaagaaaaga cggttttttt gtacgaaaaa attc
//
LOCUS       DARBEG010000087        10606 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000087 DARBEG010000000
VERSION     DARBEG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.088"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            23..646
                     /locus_tag="SCK20_002291"
     CDS             23..646
                     /locus_tag="SCK20_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146322.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="SCK20_002292"
     CDS             1096..2145
                     /locus_tag="SCK20_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1146323.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="SCK20_002293"
     CDS             2142..3023
                     /locus_tag="SCK20_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1146324.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="SCK20_002294"
     CDS             3026..3796
                     /locus_tag="SCK20_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1146325.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="SCK20_002295"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="SCK20_002295"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG1146326.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="SCK20_002296"
     CDS             5107..6270
                     /locus_tag="SCK20_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1146327.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="SCK20_002297"
     CDS             6387..6797
                     /locus_tag="SCK20_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1146328.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="SCK20_002298"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="SCK20_002298"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1146329.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="SCK20_002299"
     CDS             9370..9654
                     /locus_tag="SCK20_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146330.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="SCK20_002300"
     CDS             9814..10050
                     /locus_tag="SCK20_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1146331.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="SCK20_002301"
     CDS             10047..10445
                     /locus_tag="SCK20_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1146332.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3704 a         1771 c         2242 g         2889 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatag
//
LOCUS       DARBEG010000088        10193 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000088 DARBEG010000000
VERSION     DARBEG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.089"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            120..626
                     /locus_tag="SCK20_002302"
     CDS             120..626
                     /locus_tag="SCK20_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG1146333.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            643..1542
                     /locus_tag="SCK20_002303"
     CDS             643..1542
                     /locus_tag="SCK20_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG1146334.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1629..2513
                     /locus_tag="SCK20_002304"
     CDS             1629..2513
                     /locus_tag="SCK20_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1146335.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2705..2776)
                     /locus_tag="SCK20_002305"
     tRNA            complement(2705..2776)
                     /locus_tag="SCK20_002305"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2741..2743),aa:Arg,seq:ccg)
     gene            2836..4170
                     /gene="rpoN"
                     /locus_tag="SCK20_002306"
     CDS             2836..4170
                     /gene="rpoN"
                     /locus_tag="SCK20_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG1146336.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4319..5410
                     /locus_tag="SCK20_002307"
     CDS             4319..5410
                     /locus_tag="SCK20_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG1146337.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5451..6452
                     /gene="gap"
                     /locus_tag="SCK20_002308"
     CDS             5451..6452
                     /gene="gap"
                     /locus_tag="SCK20_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1146338.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6562..7752
                     /locus_tag="SCK20_002309"
     CDS             6562..7752
                     /locus_tag="SCK20_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG1146339.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7856..8611
                     /locus_tag="SCK20_002310"
     CDS             7856..8611
                     /locus_tag="SCK20_002310"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG1146340.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8727..10025
                     /gene="eno"
                     /locus_tag="SCK20_002311"
     CDS             8727..10025
                     /gene="eno"
                     /locus_tag="SCK20_002311"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG1146341.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2630 t
ORIGIN      
        1 ttttgtcaca ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa
       61 aattgaaact tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa
      121 tgatttttac tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg
      181 gactcatcga aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata
      241 aagaagaggc cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa
      301 aagaaaaaga gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat
      361 tagatagttg gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc
      421 cccaacagtt acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag
      481 acagtccgga aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa
      541 ttgcgactat cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga
      601 atgctttacg tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa
      661 aatttaaaaa aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag
      721 gagatccgcc agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag
      781 agacgtctcc aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt
      841 ctttttaata gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt
      901 gaattgatct taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg
      961 aagatcaata cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac
     1021 tttcgcgaag gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg
     1081 gatcattacc aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg
     1141 attgaaaggc agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca
     1201 atcgacaaga aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat
     1261 ctatctgttt tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca
     1321 tctaagtact attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt
     1381 gttacgccta cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct
     1441 caatacgaaa ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg
     1501 acaaaaacga ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac
     1561 tagcagtttc aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa
     1621 gagatatagt gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt
     1681 ttttcgaaat tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt
     1741 cggagcattc gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa
     1801 ctgaaacagc taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt
     1861 catcacaatc aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg
     1921 caatcagctt tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa
     1981 aagaaaaaaa cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg
     2041 aaatcgtgaa gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc
     2101 agaatctggc cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg
     2161 agatcaaaaa agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca
     2221 cgaatgtagg agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag
     2281 attggaagac aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg
     2341 gggcaattat tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga
     2401 tcgattatct gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc
     2461 caacggctgt cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac
     2521 aagtatcttt aataaataac acattttaat aagtagttga acacataact accttacttg
     2581 caaaggctta acaatatcta tacaggtaca acaaaaaact gtccaccaat tttctttggc
     2641 ggacagtttt cacttaaaaa taaagaagca actattcgta aattatgcta aaatagcagt
     2701 agaacgcgtc ctgagggaat cgaacccccg tctcaagaac cggaatctta cgtgatatcc
     2761 actacactaa ggacgcaagc actgggataa ttgtaactaa acagataaaa aattacaagg
     2821 ggattggaca aaaaaatgaa atttgagcaa aatttttcac aaaaacagca gcagacacag
     2881 aaactagcca tgacgcaaca gttgcaacag tccatccaag ttttacaata taacagtgag
     2941 gagctgttgg cattcgttga gaatcaagca atggaaaatc cattggtgga agttgttgag
     3001 ccggaatggc agccagatta cataaaagct tcttcatctt cttatgaagg agaagagaca
     3061 aattacctaa atcagattcc tgatacaaaa gggtctttat ttgattcatt gatcgaacaa
     3121 gtccatttga attaccgaga tacttttttg aggaaaatcg ttctttatct tgtagaatac
     3181 atcgatttga acggattttt gacgattagt ttagaagaag ccggaaaaga gattggagct
     3241 acgcctatcc aaatgctcga tgcgttgact ctgattcagc aattagaacc agctggagta
     3301 ggtgcacgta gtttgcaaga atgtctcatg cttcaaacag aacgagatga ttatgcgccg
     3361 gaattagctt atatcgtgtt ggaagaatgt tttgaggaac tggtggagcg aaaatggaaa
     3421 gaaatcgcac aacgtttttc tgttgatcta catgctgttc agcagatatt cgactattta
     3481 caaacgttga gtccaagccc tggccggatt ttcgaccgta gcagcgaatt gtacattatt
     3541 cctgatgtcc gggtattagt ggacgacgat aaaaatgtac aagttatctc aaaccgaaaa
     3601 aatcagccaa atatacgctt tcaagagagt tacttcaaac aaatgtcaca acaagcagat
     3661 aaagaaactg aaaattattt aaaagaacgg aaacaggaat tcgaatggtt gaagaaaacg
     3721 atcctacaac gtggggatac aattttgcgg gttgctcaag tcattgtttc ccggcaaaaa
     3781 gctttcttta tcgacaagga acgtccaatc aagcctctta cgttaaagga agttgcaact
     3841 gagatagatg tgcatgaatc cacggtcagt cgtgcagtca acgggaaata tttggaaaca
     3901 gacttcggtg tatttgaatt aaaaaaattt ttcactacac gaattcctac gaatagtaca
     3961 gaacagacag aagacctttc agcagataca gcgaagaaaa agctgcagga attagtcgat
     4021 caagaagaca aaaacaagcc gttgtctgac cagaaattgg ttgaattgct gaaaaaagat
     4081 gagatagcga tctcaagacg aacagtagca aaatatcgtg atttattagg aatcccgagt
     4141 tcatcaaaaa gaaaacgtta tgataactaa tcagtcaaaa gactagaaaa agtatttcac
     4201 tttttctagt ctttttagtt gaaaaacgag agcaaaactg ttagacttac agtcgtgagg
     4261 ttgagatttc cctttattga tgtgaaattc gaatagttaa tttctttttt tatcgtatat
     4321 gggtcgtttt aagtctacat tggacgtaaa gtgtccaact aaggagatcg ctatgctgga
     4381 tgaaataaaa atgattgaat ctgttgcacc agatattatc gaagtgttgc aagaacgcta
     4441 taaaatttta cgaaatatct attggatgca gccgattgga cgtcgaagtt tatcggaaag
     4501 tatggggatc acagaacgag tattgcgaac agaaacagat cttttgaaaa atctgaattt
     4561 gatcgacacc tcaaaaagcg gtatgacgct gacaactaag ggcgaagaag tttatcaaag
     4621 ccttgaaaac ttcatggatc agcttttagg tacgcatcaa accgaacaac agttggcaga
     4681 atattttggt aaccaacgct gtatcgttgt ttcaggaaac agcgaagagc agaccaaagt
     4741 ggctgatgca ttcggcgaag cgctgagcga agccctagac cgactacttc cagaaggcga
     4801 gaatatcatc gctgtgatgg gaggaactac tatggctgtg gtagccgaac aattgtctaa
     4861 tctggaaaat aagaaacggc ataacctgtt tgtccctgca agaggcggga ttggtgaagc
     4921 gataactgta caggcaaatt ctgtcagtgc aagaatggct gctaaagcaa acggtaacca
     4981 ccgtgcactt tatgttccgg aacaattaag tctggcaact tacaattctt tactcaatga
     5041 gccatctatc caagaagtct taaacttgat tagtgaagcg aattgtgtta ttcatagtat
     5101 tgggcgtgca ttacacatgg cagcacgtcg taagatgact gaaaaagaat tagtcatgct
     5161 gaaacaagca aatgctgttg cggaatcttt tggctacttt ttcaatgaaa aaggtgaagt
     5221 cgtttataag gtccctagaa tcggcataga attgcaagat ttggaaaaag tcccgatcat
     5281 catggcaatc gcgggcggaa aatcaaaagc caaagcaatt cgagcttata tgaaaaatgc
     5341 accaaaacaa acgtggctca tcactgatga agctgctgca aatgagattt taaaaggggt
     5401 aaccctttaa aataaatatt tttttgattt tcataaggag gaaatcttaa atgacagtta
     5461 aagtaggtat taatggtttt ggacgtattg gacgtctagc attccgtcgt atccaagatg
     5521 tagatggaat cgaagtagta gcaatcaacg acttgacaga tgctaaaatg ttggcacact
     5581 tgttaaaata cgacacaact caaggacgtt tcaacggaac agttgaagtt catgaaggtt
     5641 cattcaacgt aaacggaaaa gaagttaaag tattagctaa ccgcaaccca gaagaattac
     5701 catggggcga actaggcgta gatatcgttc tagaatgtac tggtttcttc acttctaaat
     5761 cagcagctga aaaacattta acagctggtg ctaaacgcgt tgttatctct gctcctggcg
     5821 gaaacgatgt tccaacaatc gtttataaca caaaccacga aacattaact ggtaaagaaa
     5881 cagttatctc aggtgcttca tgtactacta actgtttagc tcctatggct aaaacattga
     5941 acgacaaatt tggtgttgtt gaaggattaa tgacaactat ccacgcttac acaggtgacc
     6001 aaatgactct agacggacct catcctaaag gtgacttccg ccgtgcacgc gctgctgctg
     6061 caaacatcgt tcctaactca acaggtgctg ctaaagctat cggtttagta attcctgaat
     6121 tgaacggtaa attagacgga gctgctcaac gtgttcctgt accaacaggt tcattaacag
     6181 aattagtaac agttcttgaa aaagaagtaa ctgttgacga aatcaatgca gcaatgaaag
     6241 aagcttcaaa cgaatcttac ggatacaaca cagacgaaat cgtttcttca gatatcgttg
     6301 gtatgactta cggttcatta tttgatgcta cacaaactaa agtaatgaca gttggcgaca
     6361 aacaattagt taaaactgtt gcttggtacg acaacgaaat gtcatacact gcacaattag
     6421 tacgtacttt agaatacttc gctaacttat aagatagaca aacgtacaag tgtaaattat
     6481 agacatgtac atttaaaagc ggggaagcaa tcgcgcttct tccgcttttt ttgataataa
     6541 gtttattcag gaggaaataa aatggctaag aaaacagtga aagatatcga tttaaaagac
     6601 aaaaaagttc ttgtccgtgt tgacttcaac gttcctttga aagacggcgt gatcactgac
     6661 gacactcgta tcaaagcagc attgccaaca atcaactacg ttctagaaca aggcggaaaa
     6721 gcaatccttt tctctcacct tggacgtgta aaaacagaag aagataaaga aggaaaatct
     6781 ttagctccag ttgcaaaacg tctaggcgaa ttattaggta aagaagtaac attcgtacct
     6841 gaaactcgcg gagaacaatt agaagaagca atccgcaaca tgaatgacgg cgacgttgtc
     6901 gtattcgaaa atacacgatt tgaagatatt gacggtaaaa aagaaagcaa aaatgataca
     6961 gaactaggta aatactgggc ttcattaggc gatgtatttg ttaacgatgc tttcggtaca
     7021 gctcaccgtg cacatgcttc taacgtaggt atcgcttcaa ctggtatccc aacagttgct
     7081 ggattcttga tggaaaaaga aatcaagttc atcggtgaag cagtagaaga accaaaacga
     7141 ccaatgattg caatccttgg tggcgcaaaa gtttctgata aaatcggcgt aatcgaaaac
     7201 ttgattccta aagcagataa aatcttgatc ggtggcggaa tgacttacac attctacgaa
     7261 gcaaaaggaa tcaagatcgg taactctcta gtagaagctg ataaagtgga actagcaaaa
     7321 gaattaatcg aaaaagcagg cgacaaactt gtcttgccaa tcgacaatgt ctgtgcacca
     7381 gaattttcaa atgatgttga aacacaagta atcgaaggag acattcctga tggtttgatg
     7441 gctttagaca tcggcccagc atcagtaaaa ttatttgctg atacattaca aggtgcaaaa
     7501 acagttgttt ggaacggacc tatgggtgta tttgaaatga gtaactttgc taaaggtaca
     7561 atcggtgttt gtgaagcaat cgctaactta gaaggcgcaa caacaatcat cggtggcgga
     7621 gactcagccg cagcagcaga acaattagga tttgctgaca aattcacaca catctcaaca
     7681 ggtggcggcg caagcttgga attgcttgaa ggtaaagaat taccaggact agcagcaatc
     7741 aacgataaat aaacgaccaa gtttgctcaa ttctgacgag taatcgatca aaaggtgaag
     7801 ggagttttca tgactcccta gccatttttc aataaattaa ggagtgtgtt cctcaatgcg
     7861 taaacctatt atcgcaggta actggaaaat gaacaaaact gcatcagaag caaaagaatt
     7921 tgctgaagca gtaaaaaaca aaatcccttc aaatgacaaa gttgattcag tcatcggatc
     7981 tcctgcatta ttcttacaag aattaatgac atcagctgaa ggtacagaac taaaaatctc
     8041 agcacaaaac tgctactggg aaaactcagg tgcgttcact ggtgaaactt caccagcagc
     8101 tcttgctgat ctaggtgttc attatgtgat catcggacat tcagaacgtc gtgaatattt
     8161 ccatgaaaca gacgaagaca tcaataaaaa agcgaaagct atctttgcaa atggtatgac
     8221 accaatccta tgctgtggcg aatctttaga aacctatgaa gcaggcaaaa ctgctgaatg
     8281 gatcgaagga caaattacag ctggtttagc tgatctttca gctgaacaag tgtcaaacat
     8341 ggttattgct tatgaaccaa tctgggcaat cggtactggt aaatcagctg acgcacaaat
     8401 cgctgacgaa atttgtggag ttgtacgtca aacagttgaa aaactttatg gaaaagaagt
     8461 atcagaagca gttcgtatcc aatacggcgg ttctgtaaaa ccagaaaata ttgctgaata
     8521 catggcaaaa gaaaatgttg acggtgcttt agtcggtggc gcaagcttgc aagcagattc
     8581 attcttgtca ttgttagacg ctgtaaaata attgttgaaa atttgcgaaa aatgcaagaa
     8641 atgtttgcac taatgagcaa aattaactaa aatcgtatat gagggaaatc ccttaggaaa
     8701 aaacaaactc aaaggagaga caaaacatgt caattattac tgatgtttac gctcgcgaga
     8761 tcttagactc acgtggtaac ccaacaatcg aagtagaagt atacacagaa agcggagctt
     8821 ttggccgcgg aatggttcct tcaggtgctt caactggtga atacgaagca gttgaattac
     8881 gtgacggaga caaagctcgt tatggcggta aaggtgtaac taaagctgtc gacaacgtaa
     8941 acaacatcat tgctgaagca atcattggct acgatgttcg tgaccaaatg gctatcgaca
     9001 aagctatgat cgctttagac ggaactccta acaaaggtaa attaggtgcg aacgctattt
     9061 taggtgtttc tatcgctgtt gcacgtgcag ctgctgacta cctagaagta cctttatacc
     9121 actacctagg cggattcaac acaaaagtat tgccaacacc aatgatgaac atcatcaacg
     9181 gcggatcaca tgctgataac tcaatcgact tccaagaatt catgatcatg cctgttggcg
     9241 ctccaacatt caaagaagct ctacgtatgg gtgctgaagt attccacgca ttagcttcaa
     9301 tcttgaaagc tcgcggatta gcaacttctg ttggtgacga aggtggattt gctccaaacc
     9361 ttggttcaaa cgaagaaggt tttgaagtaa tcatcgaagc aatcgaaaaa gctggctatg
     9421 tacctggtaa agacgttgtt cttgctatgg atgctgcttc ttcagaattc tacgacaaag
     9481 aaaaaggtgt ttacgtacta gctgattcag gcgaaggcga aaaaacaact gacgaaatga
     9541 tcaaattcta cgaagaatta gtttctaaat atccaatcat ctcaatcgaa gacggattag
     9601 acgaaaacga ctgggacgga ttcaagaaat taactgacgt attaggcgac aaagttcaat
     9661 tagttggtga cgacttgttc gtaacaaaca ctcaaaaatt atctgaaggt atcgaaaaag
     9721 gaattgctaa ctcaatcctt atcaaagtga accaaatcgg tacattaaca gaaactttcg
     9781 aagctatcga aatggctaaa gaagctggct acacagcagt tgtatctcac cgttctggtg
     9841 aaacagaaga ttcaacaatc tctgatatcg ctgttgcaac aaacgctggc caaatcaaaa
     9901 ctggttctct atcacgtact gaccgtattg ctaaatacaa ccaattacta cgtatcgaag
     9961 accaacttgg tgaagttgca gaatacaaag gtttgaaatc tttctacaac ttaaaaaaca
    10021 aataattctt ttctaatgaa gatacatgat cgactattga aagatagtcg agtatgatca
    10081 cttagttaag taatcgaact ctctacttgc tgaaatatcg gctctttgtc aataaggact
    10141 gatgagttgt gcaaaatcaa atctgagtca gaatgaacca cattctggct cag
//
LOCUS       DARBEG010000089        10086 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000089 DARBEG010000000
VERSION     DARBEG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.090"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(247..759)
                     /locus_tag="SCK20_002312"
     CDS             complement(247..759)
                     /locus_tag="SCK20_002312"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG1146342.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCK20_002313"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCK20_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG1146343.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="SCK20_002314"
     CDS             complement(3212..3655)
                     /locus_tag="SCK20_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG1146344.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="SCK20_002315"
     CDS             complement(3656..4444)
                     /locus_tag="SCK20_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1146345.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="SCK20_002316"
     CDS             complement(4441..5382)
                     /locus_tag="SCK20_002316"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG1146346.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="SCK20_002317"
     CDS             complement(5559..6146)
                     /locus_tag="SCK20_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG1146347.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCK20_002318"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCK20_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG1146348.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="SCK20_002319"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="SCK20_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1146349.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="SCK20_002320"
     CDS             complement(9063..9998)
                     /locus_tag="SCK20_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1146350.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DARBEG010000090        10084 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000090 DARBEG010000000
VERSION     DARBEG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.091"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..272)
                     /locus_tag="SCK20_002321"
     CDS             complement(<1..272)
                     /locus_tag="SCK20_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146351.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRD"
     gene            complement(372..881)
                     /locus_tag="SCK20_002322"
     CDS             complement(372..881)
                     /locus_tag="SCK20_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG1146352.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(1082..1951)
                     /locus_tag="SCK20_002323"
     CDS             complement(1082..1951)
                     /locus_tag="SCK20_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1146353.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1971..3092)
                     /locus_tag="SCK20_002324"
     CDS             complement(1971..3092)
                     /locus_tag="SCK20_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1146354.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(3094..3489)
                     /locus_tag="SCK20_002325"
     CDS             complement(3094..3489)
                     /locus_tag="SCK20_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1146355.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(3508..4314)
                     /locus_tag="SCK20_002326"
     CDS             complement(3508..4314)
                     /locus_tag="SCK20_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1146356.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(4307..5095)
                     /locus_tag="SCK20_002327"
     CDS             complement(4307..5095)
                     /locus_tag="SCK20_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1146357.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(5109..5585)
                     /locus_tag="SCK20_002328"
     CDS             complement(5109..5585)
                     /locus_tag="SCK20_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1146358.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(5657..8314)
                     /locus_tag="SCK20_002329"
     CDS             complement(5657..8314)
                     /locus_tag="SCK20_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1146359.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(8333..8737)
                     /locus_tag="SCK20_002330"
     CDS             complement(8333..8737)
                     /locus_tag="SCK20_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG1146360.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(8969..9226)
                     /locus_tag="SCK20_002331"
     CDS             complement(8969..9226)
                     /locus_tag="SCK20_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG1146361.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(9351..9683)
                     /locus_tag="SCK20_002332"
     CDS             complement(9351..9683)
                     /locus_tag="SCK20_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146362.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         3143 a         2040 c         1400 g         3501 t
ORIGIN      
        1 tgatctctat tacctatctc actcttattt aacaaaagtt ttttcatgtc aaatttaatc
       61 ggctgtttta tttttgtaaa ttcgcttcta ctactaatcc aatccgagat taatttttca
      121 acatgtccca gagtaaactc tgatttattc gctaaatact ttttttgttg tattaaagac
      181 atctttcccc gaagctcaaa attgagccta catcgattct ctatctcttc aaaaagcatc
      241 ttatcttcat cattcttata ttcatcattc ataaaaccaa tccagctcct cgattaagat
      301 aatattttaa tctcatacaa aaactagctc tcatcaaaac aaccattgtt ttgatttctc
      361 atgaaatttc tttatacttt tctccgatta taaattccta accccttgag aaaattacgc
      421 atatatttat ctccgcaagg cttaaaattt cgttggcctt cttttctcaa aaatgccccg
      481 atttcgcctt ttgatggata gatttctgcc tcagcaaaaa cgtctaaaat atcatcagtg
      541 gtcatagaca aggcgatttt taattttttt agcaacacat tgttgatata tttgatttcc
      601 ttgctttttt gcggcgtgtc tactatcggc ttatcttttt gcggaccacg cgccaagaga
      661 atcaggccat ttaggaatgc gtcaaaaact tggttgttga tggttttttc atacacatta
      721 ttttctgcat tatctttgtt cttatcctgt actttggtca gtaccgcctg agcttcttct
      781 ttagtcagtg gtacaccgcc tagctcaaaa gctttaatca aatccgtatc tttcaaatca
      841 atcgcataac gcaatcgcaa taacctatca ttgttattca tttgttcctc cgaagtattt
      901 agtctttact ttcttacgta ctttttgtct tgctaagatt tttaggatca atgataaaaa
      961 ccgcatcact tatgataagg cgctgggtat catgagtgaa tgatattgct gaatacaaat
     1021 agtccgacca cattaagttg atcggacttc ataagcattt tattgactaa tacttttttg
     1081 cttaagaatt gattctaaca tcttttgcta aagttaagca tcctatagtt cctgcattat
     1141 ctgctaactt aggtgtgaca atgtattctt cgatatttgg cgtatcgaca tagccgttaa
     1201 ttagttctaa aaaacgtctt tgtactttct ttttcagatg ttcttgttgc atgacaccac
     1261 caccaaaaat tataatatct ggtgaaagca tcagggtcgt attacaggca cattgtgcaa
     1321 tataatccgc ctcgatttcc cagaacggat cttctataga aagatcttgt cccttggttc
     1381 ccgtacgcat ctcgatagcg ggtccagatg ccaacccttc gagacaatca tgatgaaaag
     1441 gacaagtacc agagaaagta tctcctgaat gtctcttaac taacgtatgc cccatttctg
     1501 gatggctaaa cccttcaaca aaaatcccat cttgtatagc tcctccacca attccagttc
     1561 caacagtgta ataaacaaca ctggataaac cttttccatg acctgaaaca tactccccgt
     1621 aacaagcagc attcacatcg gttgtccagg caattggaat ttcgaaatgc ttattcaacg
     1681 ttccaacaaa atcaaaattt ttccaggcag tttttggagt agaggtgata tgtccatagg
     1741 tctttgattc acgatggata tcaatcggtc caaatgaccc tactccaatg ctttctaatt
     1801 gttctttata gtgattaaag aaatcaatta ccaaactcat tgtttcattc ggtgtagttg
     1861 taggaaaact cactctttct aaaaccttta aatcatcatt gccaatggca caaacaaatt
     1921 tagtcccacc tgcttctatt gagccataca tttcttactc caccgtcctt ttatctagga
     1981 attcgttctt ccctgtctaa tagtttttcg aaaaactcat gtggttcctt ttggtaccaa
     2041 taggcaacag aagaaatatc gtcttgacgc tcataaagct tgatgtcatc atttcctagg
     2101 gtttggaaag taacacgtaa attttctttg aaactaatgg ggtcgggtaa atgccagcgg
     2161 tacaacccat gcatcggtaa agcatcatcg ccaaaaccat gaactggatt tggattaggt
     2221 tttccagcac taaaataatc ccgggtgtga tctcttttag attgaaaagg atagcccata
     2281 aatagcgtat taaaattttg agcatatgcc ctttctccaa agttcttttt atggaaagcc
     2341 caagcgccgc caaaataatc ttctgctcct gtagatgtta ttgtagggaa atcttcatct
     2401 ccatctatat aaaacttaaa ttctccttcc ccccaccagt atctttccaa tgcgcacaaa
     2461 gcaaagtagg ttcctacata atacccagtg cctttgattc catcaataag ggtgtaatct
     2521 tttccttttg tcgtgatggg ttcacggttc catttagcat gaaaatacat cgcatcatcc
     2581 ggtacttcag tcaatgcata attaaatgta taaaaaatgt gcttaataaa ctcaggatgt
     2641 tcattcgtta aagtaatctt agcttttttt cgaaatggca tctcaaaata gcaattcatc
     2701 cctgatgttg gattcactac gattggtaag gaattcactt cgcatcttgt cccaaaccca
     2761 ttacaaaaaa aatctcctaa tggactttct acagaaggag tggattcatc atcccaataa
     2821 atacgcaaaa ttaaatcacg taagacgaaa ctcccagcga ttgttttgtc tgtcactgtc
     2881 atccaaaaat gacgtaaaat tccagggcct tccatttctg ctaaagtaat tgtctctcca
     2941 gcatttatcg gaacagaagg tgaccctttt ctcccaattc ctagatgact cgctgtcatt
     3001 gccgctttcc ctttttcacc atttttattt tctggtgaaa tagttctact ttcctctccc
     3061 ttgtggagcc atatattatt taaacaattc attttattcg tcctctatcc tttcttcact
     3121 tttttgatta ttcatataga tgatttgttg ttgtgcctcc gcaataattg ctccaatatc
     3181 taaattatca tttcctaact gcaaagcgat tgacattgcc aagggaagat tcattcctgc
     3241 aattacgtgc actctgtcag aaatataggt aaaaaacttt tgatttacac ttcctccagc
     3301 catgtcagtt aaaacaatta tttcgtctgt agtatccact aactctttaa tttgttcatc
     3361 tatatcatca atttcttcga catatgcaca taagctacta atttgattat gcatacctgt
     3421 taaaaactct agtgtctctt taaaaccctc agctagtatt gaatggctag caatgatcaa
     3481 tcttctcata attcaaatcc ttttccttta cgctaatatt ccaaatgcag ccataaccat
     3541 cgcaaaaaca ataacgagta gaatcaaaat ggtcatactg atttttttct tactgagtag
     3601 ataatatgct gcagcagtaa taattactgg aattaatgtt ggtaaaattt tgtccaacat
     3661 tggttggatt ttcatactaa cttcaccaaa agtgaattta agtggcgttg aaacactgat
     3721 aactgaagca attaatgatc caactactgt tagccccaat actgaagcag cttcagttaa
     3781 ataattaatc ttttcaccgt attcagtaac aatcttcatt ccctgcttat aaccaaattc
     3841 aaagcctctc attcggaaaa agaagattac catgattaaa atgaaccata aaaagattcc
     3901 tacaggatta ttttctaaag ccatataagc cgcaattgat cccatgatgg ttgggatcat
     3961 cgtcatcaaa agagagtccc caataccagc aattggaccc atcgtaccaa ctttaaagtc
     4021 attaatcgca tcttttgctt ctaatccttg cttttcctcc attgcaacac aagcacctaa
     4081 aataatggct gccagccaag gctgtgtatt gtaatactga aagtgattat ttaaagcttc
     4141 gatgtattca tcatcatctt tgtataattt tcttaaaata ggtgacaatg cgtaagtcac
     4201 agatgcacct tgctgtgttt gataattaaa ggtacaaact gacatcaacc aacgatagtt
     4261 cgctttccta agatcctttt tcgttacttg atatttttcg ctcattttat tcatcctccc
     4321 caataaaccc aacatcagaa gctcccgcag aagcagtgat ttgtgcatct ttttcagctc
     4381 tatgatacac attaaatgct aaagcgaatc ctatgatcgc cgctccaata ataggtacct
     4441 tcaaaaaagc ggctaagaaa aatccaacaa gtaaataact aatgtatcga cctgtaggca
     4501 taaagcgtaa caacatcgca ataccaacag caggcaacat ttttccggct aatgttagcc
     4561 catccataaa ccaattaggt aaaacatcca aaacgtaatt aacagcagac tgtcctaaag
     4621 taacggcaat aaatactgga attgctgcag ataaaccaaa gaaaattggc cccaaccaca
     4681 aaacactatt cattttctta aattctcgtt tattcgcata ttcctgtgat ttgcgaacaa
     4741 caaaaccatt taagattttg acaattacat ctagttgcac ccctaacata cctacaggta
     4801 gtcctattgc taatccggct tctactccaa gctggctacc aaatgccgtt gcaataattg
     4861 cgcacgttcc atagtccggt acagatgagc caccaaaacc ggcaactcct aatgacatca
     4921 attgaactgt accgccaact atcaaacctg tttttatatc acccatcaaa attcctgtga
     4981 ataatcccca aaaaactgcg taatttacaa agatcatcgg aatcccataa taatcaacat
     5041 gtttaataaa tgctaatata gtcagcataa taatttgaaa aatagtaaaa tgcattatcc
     5101 ttactccttt atcctaattt tgattgaatt tcctgtgcct tatccgccgg aacaaattgt
     5161 gtaacaattt ctacaccaga atccaacaat tttttatacg tgctgatatt ttcatctgat
     5221 gcataggctc tagaagatag ctgtaatgca ttatctttaa caacagtacc gccaacaatt
     5281 agtcggtcaa ttctaatacc gttatctact aaatgaagga ttgtttccgg ctctttcacg
     5341 atgacaaata ctttttcctg atcgtatttc ccctttttaa aattcgcaat cgctgtttct
     5401 tcagtgataa ttgatacagc cattccagca ggtctagcta aacgcatact tgatttttgg
     5461 atgtcgttat ttgcgacaga atcatcgatc accatcactc tgtttgcttt agacattggt
     5521 gcccattgcg tgacaataat cccatgcaat aaacggttat caactctcga cataacaata
     5581 ctcataaata cttcctcctt tatctttcat tttcttacaa gcaattacca tgccaaaaca
     5641 aaaagaactt gataaatcaa gctcttttta cataatcata aacgtattta acctcatgaa
     5701 tcgggatgtc gacactataa taatgttcaa cgacactgaa agattgctta aaatgtgtct
     5761 gaaatttttg aaattcctca ctcgtttcat tgaaataaat ttcatcttcc aaagtatttt
     5821 gttttactaa tcgttcaatt aagcaactaa tgtgaacata taatccgaaa cacgtattat
     5881 tatcaaatga aattcctaga tcattttgta agatgtatat agcatctgaa acccgttcta
     5941 ataatttagt cggatttaat atggtcaatt gctccattaa attcgttaga ctaaaattct
     6001 tcagaatatt attgttcaaa actttcagtt cattatcatt aaagtgtcta ttcaattgat
     6061 tggagaattt acttatattc attcctaaaa tcatatcttc aattgaaaag aattctacat
     6121 cttctagatg tggatttagt gttccaacaa taaaaagaat attgttctct ttacgaagct
     6181 ccgataaata gttttcattc actaaagagt aatagtcaca cgtttctatg acaagatcag
     6241 tatcccttgg aaagctatct tcaagtatct tttttaattt aaacgaagtt cctaatccgc
     6301 ttgcacaaga acagacaatc atatccttct taatatttga tgattcataa atataatcca
     6361 cattttgatt ttcttttact tgttcaagaa tctgtttaag cggtaaatct aataatatct
     6421 gatttgcaac atcaatagcc attcttgttg aaacattgtt tagtagtccg aatttttttt
     6481 gagcgtctag ttccaaattt agataaattt tctccagtga acccatatca acaagtaaaa
     6541 taatttcttc atatccggta cactcttgta agtatctatt aattttgtct gcaatctcac
     6601 tacttgaagt cattagtggc atatcaatgg cgtcgtatat atatctctca acgatcctat
     6661 tagcagtact tgctatacag gttgccgtta gatctccatg agccaaaatc agtgctaaac
     6721 gtttttgctg aaattcttta ctacacatta acttaaataa aataaccaaa acattttttt
     6781 gaatttttgt taaataaata tcccctattt gaataaaacg attgactaat tgaacttctc
     6841 gactacacca actattaata tcattgatga aggaatataa tttatcgtcc gatgttaatt
     6901 ggatttgata aatccaagaa ttcaaaaaga tagacgttat ttgaagataa taatagtcaa
     6961 attctatttg aaaatctctc tctatttcct taacatactg tctaaatcga atctcttgat
     7021 aggtgggata tgttaaagct gaattatttt ggatatcaac attcttttgt ctaaaatatc
     7081 tatcatattt ctcaaaaagc tgctgcttat cagtgtcttt cttccacgtt tgataaagat
     7141 actctataag ttcgttgata ttaaaaactt cgtctacttt ttccaaatca tccatttgaa
     7201 tatattttaa ttcattctgt cttttcataa ccttcatgcc gttgaaattc acccattgtg
     7261 gtaaatgata catctgaata taaagttctt ccaaatcatc taagaaataa aaggcattgg
     7321 cacaggtcaa ttgaatgata tttttcaaat tgccaacatt ttcactaaag cgatagttta
     7381 ataaaaaatc taataattgt tgagatatca gtattttctt tgctagtctt tcttcttctg
     7441 atttaaaaaa tatcttgatt aactgttttc tttcataaag agaacgactg tctaatgttg
     7501 gaagtgttag ctttacaggt attcttctca acaaagttga taatagttta tcctcaggtg
     7561 gttctgatgt tgcaaatata atccagcaac ttgatgtgta ccagttttca ttatcaccca
     7621 tccgatgaaa tttcctcgta tccataaact gaaaaagttt ctcctgacat tcattgctca
     7681 atgcatgaat ttcatctaaa aataagactc cattattagc taattctgct aaaccctttc
     7741 ttccatcttc ggctcctgta tatgcacctt ttacatgccc aaaaaaattg gacaagaaaa
     7801 gttctggatt attggcatat tcactacaat taactgaaat gaaagatgcc ttctcttcta
     7861 tataattatt ggccaacaaa tccttataca cataatttgc taaatagctt tttccagttc
     7921 cagtctcgcc atgaagtaaa atgggtaagc catttggcgg atagtagatt gctgctttaa
     7981 tttgagcaat tacatttcgc agacttcctt gacttccgat aaccctttct attccaaccc
     8041 taaaagagct atcataccat ttttccgctt cctctgaaga gctaaaaatt tgttgatttg
     8101 gaactttctc cggtaaagaa aagaagatta ctggacgagt attgattttt ccaaattctc
     8161 catcttttac gaattcattc aaatattgag agacaatatt acgactaaca tttaattttt
     8221 cagatatttt ctgtgctgta ttgttccccc atttattttt aatctcagag ttctctatga
     8281 cataattgac aatgcgttct tttaattctt ccatcacagc acctcctcga cactattcga
     8341 attttgccga cagtattttt ccataaatat gacttgctaa aaaggctttg tttagatcat
     8401 tcaattggaa cgttgcttgt tttgcttgat ccgctaagac gttgaaaaga tactctggcg
     8461 aaatcgcata ccccaaaata tcaacgattg ttgcaatcag atcttctttt gattcttcat
     8521 ctgaaagtaa ttcacgatac aacatcgttt gtttgcttac tgtgtcatat tcctctaagg
     8581 attcatctgc taaaagaacc acttgttcca acaaacggtc ggataaatat ttatagaaaa
     8641 tcagccccaa taaataattt ttatattctg acgcatccat tttactacgg agattatctg
     8701 ccgcactaaa taacttctga tttaattctg cacccaaaaa gatacactcc tatctcttat
     8761 ttaaagttgc tatataccct cttattttag catactcagt tttagaaaaa gcaaaaaaat
     8821 aaagaaactt attcttgata acaagctacg tgacgtatat ccctaacaca aaaatagcca
     8881 tatcctttgg tttggatacg gctatttgat attactcatt caattatcaa atcacagtag
     8941 gtgtgaatct catcgggatc ggcaatatct acttctgatt aggatcaaag gtgattttct
     9001 caataaaggt gtgatacaaa gctttaactt gcttcttctc actctggata agaaagcaat
     9061 ccaaactagt caatatctgt tggacatctt tgacttgaat cggttcccct tgttgttgaa
     9121 agatccctaa gatttcattc tctcgaattt gtaactgtct tcgtttttca gtcaattcat
     9181 ttaaacgatc ttttaacatc ggaaacaatt cgggactatc ttccaaagca gcttcctgtt
     9241 tttgctctaa tagttagatt ttgcgtacca ttttcttctt ttattttcct tcgattgctg
     9301 attttaatat agtctaaagg aaatttatat ttattgtatt ctttataact tcaagagaac
     9361 ttcgtttgag tattactgta tttttttaat tccataagaa aattagttat tacctgccac
     9421 ctaatcagca aatctgaacc tgagtcaata taattaatat tattttccat ctccaacatt
     9481 gtaagtactg ttggtgttaa tatagggact ttgtacaatt gtattttttt gattttatca
     9541 tctgcacaaa ggagattttt taaatctaat ccttctttta tttcaaaagt tgaatttttt
     9601 aaatctataa gagtaaaacc aattccttca tactgaattt ctgaaaatcg aatttttctc
     9661 ccagttgata gatcaagggg cagaaaacct tttattacat tgtgagtgtc accatcattt
     9721 acaatgtcat taggaatttt gattttagaa ttctgtggag cacctattgg aagataataa
     9781 ttcattataa ttttgtagtc ttcatgagga aataaagtac ttttaggatt gctaagatat
     9841 tctcgtaccg aatctaagaa tggcttatcc attgaatctt catccccatt taaactaaaa
     9901 aacattccta caattgcttt tgcaactcta agtgaattca ttttctttat tgaatgtgtt
     9961 aaatcagact taccattctt taaacttgta ttgttataaa ggaaattttt attgtcttct
    10021 gcaaatctaa tatattcctc aacatactca ctaccaaaaa aactattaca tttactacat
    10081 aacg
//
LOCUS       DARBEG010000091        10084 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000091 DARBEG010000000
VERSION     DARBEG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.092"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SCK20_002333"
     CDS             162..392
                     /locus_tag="SCK20_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG1146363.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SCK20_002334"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SCK20_002334"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1146364.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SCK20_002335"
     CDS             1803..1979
                     /locus_tag="SCK20_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1146365.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="SCK20_002336"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="SCK20_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG1146366.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="SCK20_002337"
     CDS             2474..2941
                     /locus_tag="SCK20_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146367.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="SCK20_002338"
     CDS             3026..3910
                     /locus_tag="SCK20_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1146368.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="SCK20_002339"
     CDS             4024..4260
                     /locus_tag="SCK20_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG1146369.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="SCK20_002340"
     tRNA            4348..4428
                     /locus_tag="SCK20_002340"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="SCK20_002341"
     tRNA            4435..4506
                     /locus_tag="SCK20_002341"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="SCK20_002342"
     CDS             4631..4801
                     /locus_tag="SCK20_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146370.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="SCK20_002343"
     CDS             5021..5728
                     /locus_tag="SCK20_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1146371.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="SCK20_002344"
     CDS             complement(5788..6432)
                     /locus_tag="SCK20_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG1146372.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="SCK20_002345"
     CDS             6559..6846
                     /locus_tag="SCK20_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146373.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="SCK20_002346"
     CDS             6954..7274
                     /locus_tag="SCK20_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1146374.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="SCK20_002347"
     CDS             7355..8668
                     /locus_tag="SCK20_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1146375.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="SCK20_002348"
     CDS             8665..9384
                     /locus_tag="SCK20_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG1146376.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="SCK20_002349"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="SCK20_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG1146377.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DARBEG010000092        10060 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000092 DARBEG010000000
VERSION     DARBEG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.093"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(674..1096)
                     /locus_tag="SCK20_002350"
     CDS             complement(674..1096)
                     /locus_tag="SCK20_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1146378.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(1086..3260)
                     /locus_tag="SCK20_002351"
     CDS             complement(1086..3260)
                     /locus_tag="SCK20_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG1146379.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(3276..5618)
                     /locus_tag="SCK20_002352"
     CDS             complement(3276..5618)
                     /locus_tag="SCK20_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146380.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5640..8183)
                     /locus_tag="SCK20_002353"
     CDS             complement(5640..8183)
                     /locus_tag="SCK20_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1146381.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(8193..8591)
                     /locus_tag="SCK20_002354"
     CDS             complement(8193..8591)
                     /locus_tag="SCK20_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1146382.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8672..8956)
                     /locus_tag="SCK20_002355"
     CDS             complement(8672..8956)
                     /locus_tag="SCK20_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146383.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8980..9978)
                     /locus_tag="SCK20_002356"
     CDS             complement(8980..9978)
                     /locus_tag="SCK20_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1146384.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2867 a         2134 c         1588 g         3471 t
ORIGIN      
        1 cccactctct cgcaagtttc tgttctggac agccccaaga acactggggc gttggccgtg
       61 gggttgattc tccccctctt tcaaaagagg ctgaaagagg gggttagggg ggagttagtt
      121 ttgagacccc aagactgggg taggggttga ttttttccct tttaaatttt atgctactac
      181 tatttttagt agtgtattct ttatctgttg gctgtcggcg cttggtatgt tattcggaga
      241 cttcccgctt aggtttttga ccattttctc tcataagtgg cggtttttcc acgttgagag
      301 aaaatggtca aaaacctgga agtcggagaa tgcctttcat accaagcgcc gacagccctc
      361 cccccactta ttacatataa cctttaaata atctctaaat aaccctcaaa taatccttac
      421 taatactgaa acgtaaaaaa aataaacgga tttcaaacaa aatacgttta ttttttctat
      481 tgattttagc tcaaaatcga ttcaattcga gtgaaatttg cttcaatttt caattggttt
      541 ttgttcaaat tttgaaacat ttttgctcaa aaaatgagtg aatttgtttc aaaagaaaaa
      601 caaatacgat cgaaaaaaat ttgaactcat aacaattttt tgtttttttc aacgtattct
      661 ctttcaattt tcactaaaaa ttaggaagaa attgattcaa tgtcttttca aaaaagaatg
      721 tttttctctc aatttttttg acgtttctat catgaatcac gagaaaactt gggacacttt
      781 taacatggta acgttgtaaa aaagtttgaa gttcttcttg tcttggttct tttcttgttt
      841 caattcgata tacattccta gagaattttt tgtcgtatct ttccaagaaa gattcaactt
      901 cttggcattt tttacaatca tttcttgcta gatacacaaa aaatgattga ttcgtttttt
      961 gaagttctag tacttcttct aaattactat tgattagttt tccatttgat tcttcgttag
     1021 tttttatcag tttaaaacaa ccaaataaga ggaaactaac aataagtatc ctaacaataa
     1081 ctttcttatt tcgcatattg tttctccaaa tttggtttcc gaatatagac gactcgcggt
     1141 ggttcctgga cgtcttcagc tgttcgatac accacaaatt cgcctgttct caatgctcga
     1201 atgcgattcg gattgaattt aaagcggtct acgtctcgtt tcgtgcctct ttctcctgtc
     1261 caatcaacct ttcccatatc agaataacct gcttcttgtg tcgtcatttc cgttaattca
     1321 atgtcttctt ctgtccccat taaatctgcc caataatcaa tttctgtcgg gtcatttgtc
     1381 ttcccaataa agaacgtatt cacattccca atcaactgtt ttcgaaggac aggatcaatt
     1441 ctatcaatgt ccgcaagcgt ttgaggggca tacgccgcat gcaccccagc gcctcgtccc
     1501 ttattgacta agtctataag gttatcattt agataagaag ctggttcgtc aaatattact
     1561 aggactttcg agcgattggc tgtttctcct gcttgtttat acacttctga cgcaaagaaa
     1621 ttaatatctc ccacaagcat ttgagccaat gtccgaataa actcactata aatcaatccg
     1681 tttaacgaga tataaacaat ctttccttct ttaacaactt cttgaatatc taattctctt
     1741 tttcctccag aagtatcaaa gagttcacca agctcactat ccaataacat attgatattg
     1801 gtctgtaaat tctcagaagt cgcatacaaa taaaacgggt tcttcttatg ttcgtaacga
     1861 acaaacaacc gtttaaacaa cgtttgctcg atttctgtca tgagagaaat gttttctttt
     1921 aataatagat aatacgtttc taagtcaacg gttttacgat catgttcaac gattcgctct
     1981 tcacttaact gttccacatc ggcaaaatca cgttctgttt tgtccatcgc ttcattcttt
     2041 ttcgtttttt tcttcgtggt tttcttcttt tctacgacca ctttttcttt aaataaatct
     2101 ggatttttca actgaatacg atccgcaaac aaatgtaaga cgtttcgtgg caataaataa
     2161 tgttgtaacc aaactagatt tctttcaatt ttatggctct tttggaactc gtctaaaagc
     2221 tgaatcgtta cctgtaataa cgctttggct gctgtcgaat aatacacgct ttctgtttct
     2281 gctagattgg ttaatttatc tcgaatggca atatcattgc cattttttac aggattataa
     2341 cgcaaatcgc caccgtccac aaagacttca atcgggcgat tgtatgcctc tgccacttct
     2401 tttaacgctt gtaaagtctc tcctgctcct ttcccatcga ctaagataac ggggatacga
     2461 aacttacacg catgttctgc tagtaattgg atcaccgtcg tttttcctga acctgtagtc
     2521 gctggaataa tcacatgttg gttcaattca ctaaagttca tcacataagg gagcccatac
     2581 tcatctgttc ctaatagaat cactttggct aatgaagcca gttgtttatt ccgcaaagca
     2641 acgtctttta tgacaattgc tacctggtac gcttgctgat actctgctgt atatttttgg
     2701 gactcctgaa atttattctt ccgacgttcc ttgtattctt tactatttag caaagctgtt
     2761 tgccgttctt tctttgaaac aacttttctt ttttcaataa aagacgcata cgcaatcaaa
     2821 caacttcctg cacctgtccc acctaaaagg agggcaaaaa tcgtttgtcc attaaactgc
     2881 aacgcatgag aaaacaagtt taatggaaat aataagtaac acgacaaata ccctctagga
     2941 agaacaaaaa taaaacaaaa tagatttaat agcgtaaaac ctaacgcaaa aataaaaaaa
     3001 agagagcttc ttcgttttaa gaagctctca tactcaaagt cataaatatg tgcacgtgtc
     3061 tcttttttat ctatcataaa gacaatcgct aaaggaatac tgcctacaat aaaaaatgga
     3121 tatagacaaa cacctaataa agtgacgata aaataaagtt gttgaaaccg actttttggc
     3181 gtttcttttt cttgttttct tttgcgatcg acagaaaaca tttgctcaaa atgtgtcggt
     3241 cgttttggtg cattcatcat actatcgatt tcctcctatc gttttcgttg tttcatttct
     3301 cttcgataaa tcggttgttt ttctcgtttt cgtttcattt ttgcttgagt tgctcgaaac
     3361 tcactccgtg tcttcggtgc ttgttttggt gattttgttt gtttcatggt tgagtatgct
     3421 ttagatacgg ttgaaggcgc tttggtttgt gctgttactc gattgactgc tgacatatgt
     3481 tcccgaaccc cctctggacg tcgaacagat ttcggtgtgg tagaacctgt tgctcgtggt
     3541 acattccctt ttcttagtgg tttttgtacc gcatgttttt gtcctgcaac tgtccgggtt
     3601 ttcatcgaac ttgtttttgt tacgggagta agtgcctcct tccgacgacg acttgtttgt
     3661 ccctgacgac gttttcgatc gttcaattct gttctaggcg tatgcttctg gccataagac
     3721 ttgtgttttt gctttggatt cgctaatgac accaccattg cagaaccctg attgcgatac
     3781 ggactacgag acgcaccttt tctacttctt tctcttagac taggattgcc ttgcaattct
     3841 cgttgtaata aatcggctgt ttttcttgcc tgtttctcca ttgctgttcg ttgcatacgt
     3901 tcttgacttt gctcttctgc ttgtttcgct tgatcatgca agtctcttgc tttttgtgcc
     3961 atacgttcca tacgatttgg tgctttatga cgaggttgtt gtgcggattc acgccgaaaa
     4021 gcacttcgtg tggcttgttt cgccgtttgt ggcatacgaa cgtctgagcc acccccacgt
     4081 tttcgttgac gttcttgtct cttccgtgcg ttttcttttt ctttttgttt ctgtgctttt
     4141 ctcgcttctt tgcgttcttt caaccgttgt ttggcttccc ttctcaattt tgcttctgcc
     4201 ccaaatggat ttcccgcaaa agctaacgct cctcgaacac ccattcttcc agtaaataga
     4261 ctgcctaaaa agcctcggaa caaataaatt cccaacggcg caaacaaata aacaatgatc
     4321 aagagaattt tttcccatgg atcgtcttgt gtactactta attggaaccc gtaactaaga
     4381 aaagaagaaa aaaggataca tgcaaaacct gtcattcctt ttaaaaacgt caaaatacca
     4441 aaaactttgg catagttacc aaacacattc attgtgacaa gaaacaatcc taaaaaaagt
     4501 aagaacggca acatcggtac taacaatagt tgcatagttc caataatcaa tcgaaacata
     4561 cttaagacaa aataaacaac ggtttgaatc agattcacga caaaataaaa caaggcaaat
     4621 gctgtatttt tccaactatt gtaatattgt atgttcttgt ttttcagtgt atctgcttca
     4681 tactctgtga ctttgtcatg aattttctcc cctatttttg tacctaaatc attgtccaat
     4741 aaaagattga ttcgatcata ttcttttcca gcatatttga ctttcttctc acgaattgtg
     4801 tctacattgg ttgtgccata atttaacaac agatatggtt cataaacatt actataaaag
     4861 acccgagccg ccatcaattt gcctgccgtt tccacttttt tatccgctgt tacctcttct
     4921 gtttcttttg tttcataatt tgtttcttct tcaggtgtta acactggatt gaccttcacg
     4981 aacgccgttt ccactgtaga atctaaagaa aaaagaacat caaaaagatt cttatttgtc
     5041 ccagaatctt ttaatacgct taacccagta aaaataagga tcgtcataag aaagacacgg
     5101 agtgcttgtt tcattcgagc ctctccagca tatttgattg ccatcataat tgccgaaaaa
     5161 ctgattccaa tagttcctgc aatacttaac atattgtccg caattttttg tgttaagttt
     5221 tgaatgggtt ctcgaatggc ggaaataata tctaattcaa agagaaattc aaccattgaa
     5281 gcattgaatt ctcctaacat ttttacgcca ctccaaacaa aatttttcat cactcctgca
     5341 acattgtacg atagcttttt tttaaggttc attgactcat agtcattggt catcaattca
     5401 aacgaattct ttttgtaatt tttatacaat tgatccacat cagtcgttac ttcttttact
     5461 tctactttat cgttggcatt ctctccttca gaacgatcgg tttcagtgac acccgcacca
     5521 tcattcattt gatcggcaga aacagtagcc ccttgtagac agaaaaaaag accccctaat
     5581 aaaaggagcg tccaccattt cagagatttt tttcgcaaaa aaaccacctc ttttttgttt
     5641 tatcgttgtt tttcattttc taacattgct tgaatttttt catcttgatt ttcagtagaa
     5701 gaaacagcca ataaccaact atcaaacaac acatcaatgg caatcggttg atttcttccc
     5761 aaatgatcct ggaataaaca ggttcctgtt tgtaattgtt ccatcatttc taaattggct
     5821 gaattcactt ccatatcaaa gaaggacaat agtttctctc gtgcttcttt ttgtttcgga
     5881 cgaaacgcaa atttgtaaga aattaactct tttttatctt cttgatcgta atctttaaac
     5941 gcttgcacaa caaggtaaat atccgtatta ttcgcacgtc cttttcgcaa gctatcatca
     6001 atcaaatagc ttccttgtgc ggtatcttca tacccttttg cctcgtcata aataatcgca
     6061 gcgtcttctt ctttgtttgt agaaaagata tacgtaaact tcataatgac ttccataatt
     6121 gccattcctg caagttgttc gctatttaat cgacccgaat caatctcttt ttgtgtcgga
     6181 atgcgtattc cttgtgtccc taacacgttg atttgtgaat caaaacgtat cggcgtactg
     6241 tgctcattcc caatgagaat ctttcctaaa ccaacgctat gccctaaaat caatttagct
     6301 agttctttgt cactttttcg aatttcttcg ataacttgac ttaacgaatg cttacctggc
     6361 atggtgtaaa ctacatcgat ggcgtcttgg atcaccgttt tcttgtggct cgctgtttct
     6421 cgattggtat ctacttctcc taacattcct aacacatttt tagcggtttg aattgcctct
     6481 tcttttggta aaaacaatag aggatcaagc atccctcgac agctttcatc tgaagataac
     6541 gaaataaaat taattcgttc atacatagcc acaaactctg gtatgtgtcc gaatttttca
     6601 agtgccttta aaaagaactt ttgggtttca tttttcggat caatataaag aattttttga
     6661 cctaaaaaca tcagccataa aaaaatatat tttacaagga ccgattttcc tttacctggt
     6721 ggaccaataa tcaacgtatt tccattggta tgttccgcgc cttcaatact tttctttgtt
     6781 aaattgggat aataccatac tagtttacta gatagcgcta acgcttcttt cgcagattta
     6841 aacttttttg caataacaat ccgtcctaaa ggaaatcctt tcttatttcc tacttctttc
     6901 tttaagtcca agccataatc tgccacaaaa cctgtcgtag cgacctgctc gtaactacga
     6961 aaacgttgcg tacttccaat taagcattga ttaaacaacg ttagctgatc cgctaccgat
     7021 cgataaaatt tgaaatctgt agactcaatc gcaaattcaa tatctttgac tcgttcttcc
     7081 aattcttctt ttgaagaagc agatacaacc agcgtgacgg tagaataaca caatcgtttt
     7141 tctttgcgct tcagcttatc ttttacatcc attaaccgtt ctgtaccaaa atttaataca
     7201 tcgtctgaat ctaaaatgcc gtcaacggtc tcatattctt tttcttgttc ataaatccgt
     7261 ccagccattt tattaatttt tcgttcatca gattttgaat gctcaaatcg aagtcgaata
     7321 tgcgtatcaa ttggaaaaga taagctatcc tgaagatttt gaataaagct actcccatac
     7381 atggctaaag gagcgtccgt ttgacttaaa aacgtcgaat aatgcgtttg attcaactgt
     7441 tcaatcgcaa tgtatcctgc ttcattcgaa atgacgccct cgcaaatttc cattggcgta
     7501 agctgtcttt caggcaattt ttcttctgat cgatggaaaa aataataata aatttgtcca
     7561 atctcttggg cagataagcg ctctatcggc ttataattgg acaattccgt atagagctcc
     7621 ttttctaatg tttcatacgt tggtaacaca ctacttatag gaactcgttg tcccgtaata
     7681 cgatagataa actttttaaa aacatgctta agcaattgaa aatattcgat cggatcagac
     7741 actttcattt ctgtagtgaa acgcacaaac agatacgttc gatactcgtt taattgcact
     7801 tcgtctttta aaatctttcc tgcctgttca aagtacactt tcccaagatc tgcgcattct
     7861 ccctttacca aatctcgaat ggtttcttca atgtgcgtat ctaggttaaa ttgacgtgga
     7921 atattgataa aatgatagtc atattcatca tgtccaagta tgccttcgcc atcttgaaga
     7981 tagttcttga aaaaatcaat gtcatttaaa ggaaatactt gggaagaaag ttgatagcct
     8041 acccacacat catcattttt ggtcaaaaca aaatttcgga taatggcgtc tatcggaaat
     8101 tctaatcgag gaagcttcgt ttttttcttt ttgaactgcc aaatcccgat aataaagacg
     8161 accaaaagaa acaaaaacgc catttactcg actcactcct tccatttgtg tagggaatac
     8221 gtcacttttt catggaaaaa cggcacatac atttcatgtt caaagcgttg tttccgtttc
     8281 tgataatagg acgttcgccc aattaaatac tcgaaaaagt gtttattgtc ccatttcatt
     8341 gaaaacaatg tccaaacgag aacaccaatc ccaaaaatca taatcaacca ggactttcga
     8401 aacagtgtct gcaagaaact aatttgattc acatacgcaa tcaaaaatag gatcaaaaga
     8461 atgaagagaa ataccccacc tatgactaat tgttccatgc gaatgccatt agaaatccag
     8521 ggaatttttt gtcctttaat cccgtaaatt ttgtgagggt gcttaaactc ctttgtatag
     8581 ttgtattcca tgttcactct acctcctttt acaaaaaaag aagcgaccag aagttctaat
     8641 cttctgatcg cttttgcagt ttgtcttttt cttatgataa ttgtatccag ggcacaatca
     8701 tttgcgttac ccccacgttc tcttcactat ccttaataaa gataatgttc tttagccatt
     8761 caccaatatc aacggcattc gataacaata agacgagcat tatcaaaata aagacgccaa
     8821 taatggcaaa cgctgcccgc gcaaagcctt gggtcgcata agcaccaacc attagaacaa
     8881 tcgcaacgat tgccaatagc caaatgactt gtccagatac atcatcaaat aacgatttaa
     8941 gatcaaaaag tttcatttcc attctcctct aagttcattt tattcattta acgtagtaac
     9001 aaaataacta tcgccacttg tttttacttc cattgaaaag tttgattgga actcgtttcc
     9061 tttctcaaaa tagaaacgat acttcccttg caaaaagtag tttccttctt tattttttac
     9121 tgcatgatcc agttgtacat tgatcaattc gccgccttct aacccttttt cgttagaaat
     9181 caatgccaat ttttcatcat ttttgacata taactcaaaa aaccgtttca caaatgcttc
     9241 cagcttgatt tcttcgcttt cagtgaccgc ttgcccagaa gaataaaatc gattttcctg
     9301 atagttcaca gaattggtat tcttactctc agaaagattc aaaaattgaa cgccatcaag
     9361 tacttttaaa acattgtttt gataactgac tggaaaagaa agttgactgc ttttccaatg
     9421 ttctccttct gtaaaagtaa cctcataaaa taggcgatac attgcttgtg tcaaatcgat
     9481 ttcttctagt ttaataaagc gaacatcttt cgctactcga ttggtagccg tcgttacaaa
     9541 gcctagatca gacgcttcca atccttgtgc taaataatga ttgacggaag cctcccattc
     9601 gtttcgtttt tctggcgaat aagtaaataa cctttcaata aaatgttgtc cctcgtactt
     9661 caattgttcc gtagaagaca aggtttggtt gacttcagtt aacaatgttt ctggattgac
     9721 ttgttttttc actgctaaac gcgttaatgt atccactcgt ccaaagttta ggaggagcac
     9781 gacacccatt aacagaaaca aacaaaaaaa agcgcctgaa aatacactca tggcactttt
     9841 ttgcgaaaat tggatcattc gatctgacgg ttctgttttc tttttttgga acaaaaaacg
     9901 tttcttttgt ttcttttcct tctgttgttt cggtggctta gtttggaacg cttgtggatt
     9961 aaattttgca ttactcatga gaaatgccat accgttctaa caataatttc ggtattttgt
    10021 ccacaatcac tacttcgtgg acaaccacac ttgccccctc
//
LOCUS       DARBEG010000093        10047 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000093 DARBEG010000000
VERSION     DARBEG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.094"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            44..823
                     /locus_tag="SCK20_002357"
     CDS             44..823
                     /locus_tag="SCK20_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146385.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            869..1813
                     /locus_tag="SCK20_002358"
     CDS             869..1813
                     /locus_tag="SCK20_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1146386.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1829..2608
                     /locus_tag="SCK20_002359"
     CDS             1829..2608
                     /locus_tag="SCK20_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG1146387.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2620..3318
                     /locus_tag="SCK20_002360"
     CDS             2620..3318
                     /locus_tag="SCK20_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG1146388.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3346..4110
                     /locus_tag="SCK20_002361"
     CDS             3346..4110
                     /locus_tag="SCK20_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG1146389.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4154..5605
                     /locus_tag="SCK20_002362"
     CDS             4154..5605
                     /locus_tag="SCK20_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1146390.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5637..6773
                     /locus_tag="SCK20_002363"
     CDS             5637..6773
                     /locus_tag="SCK20_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1146391.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6773..7879
                     /locus_tag="SCK20_002364"
     CDS             6773..7879
                     /locus_tag="SCK20_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1146392.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7885..8907
                     /locus_tag="SCK20_002365"
     CDS             7885..8907
                     /locus_tag="SCK20_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG1146393.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            8929..9984
                     /locus_tag="SCK20_002366"
     CDS             8929..9984
                     /locus_tag="SCK20_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1146394.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
BASE COUNT         3691 a         1425 c         2073 g         2858 t
ORIGIN      
        1 ttatatctta atttatctat catcaatact taggagtgaa tctatggaaa agccattcgt
       61 caagtcattg actgctgtgg cggtaggtgc aggtgctatt gcgatctgtt tgtttggata
      121 ccatatcaat aatcagcggc aacatcagca acggatcaat tatgcagaat ctgccatcac
      181 caatcaaaaa gacaccttga cttctttatc aaaagaagta gacaagctgt atagtacaaa
      241 agaaaaaatt tttctaaacc ctgaaataac agaagaaaca atcacaagtt tgtcaaataa
      301 gctatcctct gtcaaacttt cagcagatga ttacgacatc aaagaatcag aattgcctaa
      361 agaagctgtt gcaatccaag atgagaagaa agcaatcgtg acacagttag atagtgcaga
      421 atcaaaactg aagatccaga cagctgtcaa cgatttattt actaaaaatg tatcaaactg
      481 gcagcaagct gtagacgatg tcatcatcaa agaaaaggtg acaagtgcag atgtcgctcg
      541 tgtacgtgaa aatatgagct tttttaaaga tagtccttgg aaaacggttg ttatgcaata
      601 tctaggattc gctgatattc agatcgcgca agtcacgcaa ctagatcagt tattcgatac
      661 gatgttaaag gatggtcaag tgactgccac cgctacttac gatcaatatt tgacggtgct
      721 cagtcaaata gagcagatcc ggaatgaaaa aataagcgca gcatacgcaa caaaagcaca
      781 aactgttgct gagcaaatgg gctatagcaa tataagctat tgacagttaa aacgttgatt
      841 caagcaaggt tagcaaacgg aggaaactat ggcaagacga agaagaaaat caagagggaa
      901 aaaaatattt ctgattattt tagcagttct tatcgtatta gtattagcaa ttgtaggagt
      961 aggagcaaaa ttatatctgg atctctcaca ttctattgag aaaacttacg aatcagtgga
     1021 acgaacccaa ggagaacgaa cgaaaccagt tgatctggac aaacaagaat ctttctctgt
     1081 tctattgatg gggattgata ctggagattt aggtcgggtc gatcaaggaa gatcagatac
     1141 gatgatggtt gccacagtca gtcctgaaga caaacagaca acgatcgtca gcattcctcg
     1201 tgatacgtat gtcgacatcg taggtcgtga cacaaaagac aagatcaacc acgcttacgc
     1261 attcggcgga gctgccatgg cgatggatac cgtacaaaaa tatgtagaca tcccaatcga
     1321 tcactatgtc tctatcaata tgaaagggct aaaggaactc gttgatgcag ttggtggtat
     1381 cgaagtcaat aatgacctca ctttctcaca agatggctat gactttccaa tcggtaaaat
     1441 tacattgaac ggggatcagg cattatctta ttctcgtatg cgttatgaag acccaaatgg
     1501 tgattacggt agacaagggc gccaaagaaa gatcgtagaa ggcgtagcga aaaaagtatt
     1561 gagtctaaac ggtgtcagca actatcaatc cgttttgaat gctctagagt cgaatatgaa
     1621 aacagacatg aactttgatg aaatgaaaaa aattgcttta gattatcggg aggcatttgg
     1681 tacgattaag caagatcagc tgcaaggaga aggcttcatg caagataata tctcctatca
     1741 aaaagtcagt gatcaagaac ttgatcgtgt gaaaaaagaa ttaaaagatc aaatgaatct
     1801 agagaataaa taggaaagtg gagaaaaaat ggaagagaca atcagcttga aagaaatttt
     1861 tgaaacatta cgcaaacact tggtaataat catcatctca atgtttgctg gcttggccat
     1921 ctcaggaatc gtaacatttt tcatcttgac accaaaatac agttcacaag cacaattgat
     1981 cgtgacactg ccacaatcag acactgcaaa tgtcaatgat gtcaatacga acctgcaaat
     2041 gatcaatacg tataaagata tgatcgtcag tgatttagtg ctcaatgaag tcaaagatcg
     2101 tttagaaaca gaagacaatg tgaaaatgac tgctgaacaa atcaaagatg ccatctcagt
     2161 taatcaggca gaaaattcac aaatgttctc gattcaggca ataagtacga atgcagtggc
     2221 tgctcaacag atcgctaaca caacttcaca agttttccaa gagaacgcaa aagatgtgat
     2281 gaatgttgat aaaatctcga tcatttcagg tgcagttgcc aatacgacac ctgtttcgcc
     2341 aaataataaa ttgaatttaa tgactggctt aatgttagga atgatggtag gtgtaggtct
     2401 ggcattctta ttggaattac tcgatcggac agtgaaagat gaaaaatatg tgacagatac
     2461 attaggattc ccaatcttag gaacagttcc agaaatgtca gcgaaagaat tgaatgctgg
     2521 tatccgtaaa aaaggaccag ctcagacaca accaaaagct acaaacgata aaacagcgcc
     2581 atcacgcaga agccgctcaa gagtctagga ggaaagaaaa tggcacgaac acagaaacaa
     2641 aaagcagccc aaactcatgc agtcagtttg gtcacactca ccaatccctc ttcaccaatc
     2701 gctgaacaat atcgtacgat tcggacgaat atccagtttg cctccgcagc tggtcagcaa
     2761 atcaaaacga ttgttgtgac ttcatcagga cctggagaag ggaaatcgac cactgcagca
     2821 aacattgccg ttgtttttgc caaatctggt caacgggtgc tgctagtcga tgcagactta
     2881 cgtaagcccg tcatctataa aacgttcaag ttgaacaacg caagcggatt aagtactgtt
     2941 cttagttcat caggaagtgt agcagatgag atccaacgaa caccagtcga caatttgtcg
     3001 attctgccaa gtggacctaa accaccaaat ccatcagaac tactaagttc gccaagaatg
     3061 gatcagattt tagcagaagc aagacaacta tttgatgtag tgatcttcga catgccgcca
     3121 gtcgttgccg taacagatgc acagatcatg tcttcaaaaa cggatggcac cttattggtc
     3181 gtacgggaaa atacgtcacg aaaagaatca ttgactaaag caaaagaact gttagatatg
     3241 gtacaagcac gagtcttagg tgtcgtatac aacggagcag aacacagcaa agatgctggt
     3301 tattactacg gaaattaagg aataagaaga gaggagacaa gagaaatgat cgatctacat
     3361 tgccatatcc taccaggaat cgatgacggg gcagaaaact tagaagcaag tattgcaatg
     3421 gctgaaaaag cgattcagca aggcattacg catattttat gcacaccaca ccacaataat
     3481 ggaaaataca gcaatgaaaa atcacaagtc atttctctag tcgcttcgtt acaagccgaa
     3541 ttagagaaaa gacagttgcc tttgacgtta ttggaaggac aagaagtacg tattaccgga
     3601 acgctcatcg aagacatcca ccgagatgaa attttattta cagatttaga tgatacatat
     3661 cttctgatcg aatttccgac actggaagtg ccgttatatg cagaacggct tttttttgag
     3721 ttgttagaac taggaaagat accagtgatc gtccatccag aacgcaacgc gcattttcga
     3781 gaagacccaa atcatttgct tccgttccta gatatgggat gtttggccca acttactgcg
     3841 ccaagttatg ttggtgtatt tggcaaagat atccagaaaa cagccaaaac aatggtcaaa
     3901 cataatctcg tgcagatggc tgcttctgat gcacacgggg tctctaagcg caccttttac
     3961 ttaaaagagt gctatgagca gattgccaag gagtttggta aggaaaaagt agtacagatg
     4021 aaacaagtcg caaaggatct gatcaacggg gatgagatca attatccaat ctatgaagag
     4081 gtaaaaaaga agaagtttgg tttattttag ttctttattc gtagataaaa aggaaatgag
     4141 gagagagtga gagatgtata gaaaaaactc aagtggttgg tctaaacata ttgattttat
     4201 tttattagac ttattttgtg tccagcttgc tttttatata tcatatgtaa tgcgacaggg
     4261 tgactggaat ccgtatgtga ttcctattta tcgccacatg gcaattttta tagaacttga
     4321 gaatcttacg attatatttt tatttgaggc atataaaaat gttttaaaaa gaggatatta
     4381 taaagaggct gtagcttcta taaagcaatc ttttatgctg ttacttgtgt gcagtttata
     4441 ccttattaca atgcaggatg gaaatgatta ttccagagtt tctttattta taatgggggt
     4501 ggtgtatgga ttattaacat atataattcg tatactttgg aaagggttgt tgcatcaccg
     4561 catgaaaaac ggtgaagatg tatcgttagt tattgtaaca tctcaaaaga ttgcaaaaaa
     4621 tgtagttcgt aatcttaaag agaagaatta tggcatgtat aaacttagtg gtgtgattgt
     4681 agtagacaga gatatgtatg gaactaccat cggaggtgtc ccagtagttg caaatgctga
     4741 taatgcagcg gaatatcttt tacagaattg ggtagatgaa gtattcatca atgttgacga
     4801 aagtgttcca tatcccaaag aattaatcgc cagatgttcg gaaatgggac ttgctgtaca
     4861 tatgaatctt acaaaagtgt ctgatagtac acgtggaaaa caatttattg gccgagttgg
     4921 tgattatact gttattacat ctagtattaa tgttatgacg atgaggcaag catttataaa
     4981 gagaacgatt gacattcttg ctggaattat tggatgtatt gcaacaggtg ttatttttat
     5041 cttcgtagca ccagttattt acatcaaatc accagggccg attttctttg cacaagaacg
     5101 tattggtcaa aatggtaaga catttaagat gtataagttc cgtagtatgt atatggatgc
     5161 agaagaacgc aaagcagagt tgatgaaaga aaataagatg agtaataatc tcatgtttaa
     5221 gatggatttt gacccaagaa tcattggcaa taaaatactt cctgatggaa caaagaagac
     5281 aggaattgga caatttattc gatctacatc attggatgag tttccacaat tttataatgt
     5341 cctaatggga aaaatgagcc tggtggggtt tagaccttgc ttgaagtcag aatatgagaa
     5401 gtatgatttt catcatcgtg cgagaattgc gatgaaacct gggattactg gaatgtggca
     5461 ggtgagtggt agaagtgata tcacagactt cgaagaggtt gtaaaacttg atgcagaata
     5521 tatcaggaat tggagtatgg gattagattt tagaattttg tttaggacag tcaaaactgt
     5581 gcttcataga aacggttcca tgtaagataa agatactata gaaggagtaa aacgtgatgg
     5641 caagaaaaaa attaaaaatc tgtatgctag gtcataaaag aattccatca agagaaggtg
     5701 gaattgagat agtggtagaa gaattggcaa cgcgaatggt tgcacttggg catgaagtga
     5761 cttgctataa tcgaagtggt catcatgtta gcggaaagga atttgaccag cataaaaata
     5821 aagaatataa aggtgtaaaa ttaaagacta tttttacatt aaatataaaa ggaattgcag
     5881 caatgagttc ttcagtattt ggtggaatta gagcagcatt cggaaaatat gatgttgttc
     5941 attttcatgc agaaggacca tgtgctatgt tgtggttgcc aaaacttttt gggaaaagat
     6001 gtgttgcaac aattcatggt ctagatcatc aaagagaaaa atggaacaag cttgctagta
     6061 catacatcat gttaggggaa aagtgtgctg taaaatttgc agatgaaata attgtactta
     6121 gtgaaagcgt ccaaaattat tttgaagata tatatggaag aaaaacaagg tttataccaa
     6181 atggcgttaa aaaaattgaa ataaaatcag caggattgat tacagaaaag tatggattaa
     6241 caaaagattc ttatattttg tttttggggc gattggttcc cgaaaaagga attcggtatc
     6301 taattgaggc atttaaagat gttcagacag ataaaaaact tataatagcc ggaggaagct
     6361 ctgatacaga tgaatttgca aatgaactaa aggaattagc aaaaggtgat gagcgaatta
     6421 tatttactgg atttgtacaa ggacaggaat tagaagaatt atatagtaat gcgtatatct
     6481 atacacttcc aagtgattta gaaggaatgc cattaagttt gttagaagca atgagttatg
     6541 ggaactgttg tatagtttca aatatctcag agtgtacgga agtagtagaa gacaaggcta
     6601 tgatttttaa gaaatcggat gtaagtgatt taaagataag attagaagag gcatgcaatc
     6661 aatcggaaat ggttaaagtt ttgaagaatc aagctactga gtttatttgt agtaaatata
     6721 attgggataa aatcgtacaa gaaacattaa atttgtatag gggaaatata taatgattcg
     6781 aatattacag tgtgtgaatg atatgcatcg tgcagggttg gagacgatgc taatgaacta
     6841 ttataggaat atagatcgaa ctaaaattca gtttgatttt ttgacacatc gtccatataa
     6901 aagtgattat gatgaggaaa taataagtct tggaggaaag gtctattatg caccgagact
     6961 ttatccacaa aattatccta aatattttaa atggatggaa caatttttta aggatcatcc
     7021 agaatatgag attgttcatt ctcatattga tgcgatgagt tatttacctt taaaagctgc
     7081 aaaaaaagct gggattccga tacggatagc acatagccat aatacatcga tagatagaga
     7141 ttttaaatat cttttaaagc aatattatcg atgtaagtta acgtctgttt tgacacatga
     7201 attttcatgt ggaaaagtag caggtgattt tttgtttcga aatgataaat tcactttgat
     7261 tccaaatgca gtggatgcta aaaaatttta ttatgatgaa agtattagaa atgatgtacg
     7321 aatgaaatta ggaatttcaa gtcagacgtt tgtaatggga catattggaa gaatttcata
     7381 ttctaaaaat catcgttttt tgattgaaat ttttaaagaa tttcacgcta ttaagaataa
     7441 ttccgtatta ttaattattg gaactggtga tatggaagaa gaaataaaaa attatgcaaa
     7501 gaaaagtggc atagatgatg atattaaatt tttaggaaat agaaatgata tagaaaaatt
     7561 ttatcaagca tttgacgtat taatgttgcc atcattattt gaaggggttc cgttagtggg
     7621 tattgaagct caatttgcag atttaccgtg tttcttttcg gagaaagttc ctacagaagt
     7681 tgctttcagt gataagacca atttcataag tttaaatcaa tcggcaaaag aatgggcgag
     7741 agagattgca aatgtagata tctctcatcg agattcagaa cgttcgattt taataaaggc
     7801 agattataat attcaaacag catacaaaat cttagaatca aaatattatg agttatttga
     7861 attaatacaa agaggttaaa gaatatgaca cagaaacatt caagtataaa aagaattata
     7921 catcgcatat ccgaagaatt gaatgcaaca agacgaattg caaagataac atcttggaag
     7981 gaagcatttg ccacgttaca agctaaaatt gatattcaag tcatgaatcg taatggatat
     8041 aatgaaagta aaaaacagaa ggaacattta ttaaaaaaac atgaggtaat gctgaagtat
     8101 tatgaaaaga ctttttcaga ttttttgaaa acatatgatt atagtcaaaa tcaaataatt
     8161 aatcaatcaa aatcaccata ttctaactgc atttgggttt gttggtggca gggattggat
     8221 caatcgccgg aaattgtaaa agcttgtgta aaatcaataa aacgaaatgc agggaaacat
     8281 actgttataa ttttaacaga agataattat agacaatatg ttgatatacc aaaatgggtg
     8341 gaagaaaaga aaaacaaagg aatcattacg agaacaaatt attctgattt attaagatta
     8401 tctttgctag caaaacatgg aggtttgtgg ttagacgcca cattttattg tacaaaacca
     8461 ataccagatg agtattttga tatcgatatt tggtctatta ggaggcctga ttatgctcac
     8521 gcatccgtag catcaggata ttttgcgggt tattcattag catgtaatca agagtctaga
     8581 tggatgtttg ctataattag agactttttt ttaaattatt ggaaaaataa tgatatgatg
     8641 gtggattatt tgatgataga ttatatgatt gttttagcac agagaataaa tcatgaaata
     8701 gcacagacat ttgataaaat acctagtaac aatccattat gtgacgaatt gattaaaaat
     8761 ttaaataata tatatgatga aaaagtatgg aacttattaa agaaagatac tttactgttt
     8821 aagttgacat ggaaacaatc ttttaaaaag caaaaagatg ataaagttac tttttatggg
     8881 atgttggtag aaaacaaatt attataaata ttagaggtat aaaaagttat gaaacacaag
     8941 gtaagtgtga tagtaccaat ttacaatgtt gaaaaatatt taagaaaatg tgtggatagt
     9001 ttattgagtc agacattaaa agagatagaa atcgtattag ttgatgatgg ttcgccagat
     9061 gcttcggggg aaatagcaga tgagtatcaa aaaaaatatt ctaatgtaaa aacaatacat
     9121 agagaaaatg gggggttagg accggcaaga aatacaggaa ttgagaatgc gacaggagaa
     9181 tatgtggctt ttcttgattc agatgattgg gtagaaagtg atatgtatga aaaattatac
     9241 ttagttgcgt ccaaagaaaa tgctgatata gtagttagtg gacattgtga ttttgcgaat
     9301 gacaaagcta tggtgaaaaa gcaacatcct ttgagtggga atgtatatga tacgcctaat
     9361 agtattaaaa atgtacgaaa aaatttattt ggacatagtc caaaagatca agaagtagag
     9421 gccttcccga tgtcagtatg tatgtctttg tataataaaa atctgctaga taaatataga
     9481 ctaagatttg aaaaaatttt atctgaagat acaattttta acttgaatgc atatgatgtc
     9541 gcaaataaaa ttgcttttac agaatataca gattattgtt atagaaaaga agaacaagag
     9601 tctattacaa aaagtttatc tccaaataag cagacggaat ttaaagaatt tttaataact
     9661 ctaaaagcta tagcatcgaa agaagattta gaatgtcaaa tcagagcaaa acgtatggca
     9721 attgattatt gtaggcttta tgtgggctta gttgatgatg caaatttggg aattaaagag
     9781 aaaagaaagt atataaaaaa atttattgaa gatgagggat taattcaatg ttggaaggga
     9841 tatcctacaa atacgctacc atttcaacaa aaattttttc ataaaatgct tgaatgtaag
     9901 tgttatacaa tattgttgtt gttaagtaga attagaaaga taattaaaaa tatgaccaga
     9961 aaaaaattgt ttataatatt ataaactaaa agtatatcaa ataatagaaa ttaacaagtt
    10021 acaaaaaaat attataaaga ataatat
//
LOCUS       DARBEG010000094         9927 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000094 DARBEG010000000
VERSION     DARBEG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.095"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(393..617)
                     /locus_tag="SCK20_002367"
     CDS             complement(393..617)
                     /locus_tag="SCK20_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146395.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            808..3585
                     /locus_tag="SCK20_002368"
     CDS             808..3585
                     /locus_tag="SCK20_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1146396.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3666..4085
                     /locus_tag="SCK20_002369"
     CDS             3666..4085
                     /locus_tag="SCK20_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG1146397.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4097..4567
                     /locus_tag="SCK20_002370"
     CDS             4097..4567
                     /locus_tag="SCK20_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1146398.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4584..5363
                     /locus_tag="SCK20_002371"
     CDS             4584..5363
                     /locus_tag="SCK20_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1146399.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5350..6171
                     /locus_tag="SCK20_002372"
     CDS             5350..6171
                     /locus_tag="SCK20_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1146400.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6188..7237
                     /locus_tag="SCK20_002373"
     CDS             6188..7237
                     /locus_tag="SCK20_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1146401.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7250..8254
                     /locus_tag="SCK20_002374"
     CDS             7250..8254
                     /locus_tag="SCK20_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1146402.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8887..9906
                     /locus_tag="SCK20_002375"
     CDS             8887..9906
                     /locus_tag="SCK20_002375"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1146403.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3330 a         1644 c         2075 g         2878 t
ORIGIN      
        1 tgagaaaaat atttattcgt ttattgtggg tactatctta gaggaataaa taatagtgga
       61 gaaaactatg gtttggcgca gattctatca ggtttgatgg aacgaagaca acctcccgac
      121 atcaagtcaa aagctacttg agattttgat ctgaaaataa aaaaagaaaa caaggtagga
      181 acatcaatgt ttctaccttg ttttcgtaat caatgggaaa aaactaagag tggtgtacgt
      241 aatcaataaa aaggatggtg acaacagtac ctctttactc tctgaaagca tcataactcg
      301 tttttcctca ttatgccaat aaattgttgt tataccacat ataaggattt gtaatagcta
      361 aaaacagaca ggaaagttga aatctgtgaa tcttattttg tatggaagga tttatagaac
      421 gcaaaaatca agataaagat gaataaaaat cctgtttcgg ctatatattg cgtatctacg
      481 ccaataaaac gagtttgttc tgtcatagcc atataaagca aaggcaaaaa tacaactaca
      541 gcgagataag atccgatgat ttctcgaaaa gttttccagg aagtatgttt cctgttttta
      601 tatttatccg atttcattga atagtcatag cctccttatt ctttcatatg ttgaaattcc
      661 agtataaaaa ctggaaggaa ctgtacgttt tttagaataa aaattataag ggataagtag
      721 gctgctgtct ataataagat atatttttca attctttatt aaagatttta ttgacaaaat
      781 gaatccgttt tctatgatgg aggaggggtg aataagttgt tgaagaagga aattttatgt
      841 tacttagaga accagacagc ctttttcgat ttagaacata tgaacgaggt ctttacagca
      901 caaaggttag ctgatcattt tggggtaaaa agaaacacag tcagtcatta tttgaaccag
      961 ttaaatgatg agcaactact agtcaaaatc aattctcgac cagtgattta tttccacaaa
     1021 gaagcttttg aaaaacaatt ttttaagtta cgcacgaatt tttatttttc tatcgatgat
     1081 ctcaaagagg aacagccttt tttcgcgcaa ccaaaagact tattctcttt tatgatcgga
     1141 tacgacgcca gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta ctatccagat
     1201 ggcggattac cacttttgat tacaggcgaa agtggcacag gtaaaagtta tttagttaat
     1261 ttggtctatc agtattgtct gcttcatgat ctgctagaag attctgcacc attcatcact
     1321 tttaattgtg cacaatatgc agataatcct gaacttttga ctagcaattt gtttggacat
     1381 gttaagggag cgtacactgg tgcagaagaa aataaaaaag gggcttttga agcagcagat
     1441 ggtgggatct tgtttttaga cgaggtacat cgcttaacac cagaaggtca ggaaaaactg
     1501 ttcacctatc ttgatcaagg tgtgatttat cgtatgggag aacctaattt gtcacgccgg
     1561 atcaaaacac gtctcttttt tgctacgact gaagagatta ctagtcattt cttaacaact
     1621 ttcattcgaa gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat
     1681 gaacggctag aactgattta ctcttttttt ctcgaagaac aaagaaaaat ggggagccct
     1741 ttgtcagtca gtggaccagc tttgtcactt ttaacaagtc agaaattatc cggaaacata
     1801 ggagagctga aaaatatcgt caaagtttca gcagcaaaag catatgctga acagcgtgaa
     1861 cagcctgaaa tccatgtcac gatccatcat ttgcaaaaag aactacttgc gcagcctatt
     1921 actaaaaata cagcacagca aggaatctat attacgcaag accagacctt agagcaatta
     1981 atggagaagc aacagcccga acagcagcga atcgttaaaa gttttgagca aatcctaatt
     2041 gatttcaaga aaaataactg tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg
     2101 attcaattat ttgatttttt gctgtttgag accagtagac aagaaaaaca cgagctgctg
     2161 gtttattgga cacaatctat ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc
     2221 aaattcgatg gaaacagtgt ctatgccttg agttactatc tctatcagcg aagcactatc
     2281 aaatggttac cggaagaaca tgatattatc caactaatca aagaattaga acaacaagtt
     2341 tctcagtctt atccatctag ttatcactat gtccaacgaa ttctggaatt aagtaagatc
     2401 aaattagata ttgaagacac aagtatggat cggattttac tgacgatcta tctaaaaaaa
     2461 gcgaaatggt caaaagaaaa acgactgcca aaagcgatga ttgctgctca cggttatgca
     2521 actgctagca gtatggcaaa tgtagtaaat cgtttattac aagaggatct atttgaatca
     2581 tttgatatgc cactagatgt cacaccacag cagattgctt cagaaatcct tgattattgc
     2641 gaaaacagcg atgtttcaaa tggattgatc atattagtag acatgggatc tctgaaagaa
     2701 atccatcagt tttttaaaaa acagttatcc gtaccgcttt tgattttgaa taatgtgacg
     2761 acacctttag caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt agaagagata
     2821 gcagaagaga ctgttcgaca gatccagcca gaatggcgac tgctctatcc agaggaaaac
     2881 aaacccaaag cactcattac tacttgcttc actggaatcg gtacggcggt ccacttaagt
     2941 gaactgctag aaaaaagctt acctacggct tgtcagttga aaatcatccc atatgaatat
     3001 cagcaattaa aagataagaa aaacagtgac ccattatttt ccatatacga agtcgtagga
     3061 atgattggca ccacagatcc tgatatcacc aatatcgact atctttcatt agaagacctc
     3121 atctcggggg aaaagatgtc tgtactagct gaatggttgg acactactat gaatgcttca
     3181 gagaaggaaa tattcaatca gcgcatcatt cgtaattttt cattggaaaa agtactggat
     3241 tccgtgacta ttctagatac agagaaggtg atgggagaaa tcgatcattt tatgagagaa
     3301 ttagaggtgc agttgaatcg aagcatcagc aatcccaaaa aattagcgtt gtatgtccat
     3361 gtgagctgtc tcattgaacg attgatccga caaatgccaa tcgaaaccta tcaaggatta
     3421 gagaaatgga agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc
     3481 attgaagagc attatagtgt catgatcccc aattctgaga ttgcctatat ctatgatatt
     3541 ttgagtgaag atacagagtt tctctcgata gaagaagaat tttgaacata agcaagttgt
     3601 tgccactaat tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa agaggagatg
     3661 aaaaagtgga tagagaaatt atcttagctt cccatggaaa atttgcttca gggatattag
     3721 attcattaga gctgatctat gggaaaaatc attctattac tgtattcgat tgctatactg
     3781 atgagaattt tgatttagcg gaaacagtaa ggcagctttt tatgaggtac aaagataaag
     3841 aaatcattgt tctgacggat ctttttggcg gaagtgtcaa caacgaattc ttacaatata
     3901 tcaatcggga tcatgtctat ctcgttgccg gattgaattt gccactattg attgagctag
     3961 tatctcggtt ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg ctaactgatt
     4021 caaaaaaaat gatccagttt tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat
     4081 tttaggagga agaaaaatga ttgtattgac aagagtcgac catcggttat tacacggaca
     4141 agtagctttt tcatggacac aaacaatagg cgcagactgt attttgattg ctaatgatga
     4201 tgtacctacg aatgagatac gaaagacaac gatcaaatta gccaagccac aaggagtcaa
     4261 actagtgatc aaatcaattg atgactcgat tgccgcgttg aaaagcgggg tgacagataa
     4321 atataagtta ttcatcgtag tggaatcgat cgaggatgca tataagctgg cagaagcgta
     4381 tccagaaatc aaaaccatca acttaggagg gatcaaggca aaacctggta ctcgcagcat
     4441 cggaaaagcg gtaaatattt taccagaaga ggaggaacgc ttgaaacgat taattgacaa
     4501 acaaatagaa gtagaaatcc gtcaagtacc agcagataaa aaagtggatg taagagaagt
     4561 tttgtaagaa aggaagaacc tcaatgattg tacaagcagt cttattagga attgttgcat
     4621 ttgtcgcaca atcagaatat gcactaggga catcgttgct ttctcgtccg attgtgacgg
     4681 ggctgtttac aggaatcgtg ttaggtgatg taaaaactgg gatcattatg ggcgcaactt
     4741 tagagttagc atttatcgga tcgttctcag taggtgcttc gattccacct gatgttgtga
     4801 ccggtgggat tttagggacg gcttttgcta ttacagctgg agccggtaca gaaaccgcgc
     4861 ttttactagg tttgccgatt gctacgttga cattgatatt gaaaaatatt tacttagggc
     4921 ttttgattcc aattatgaac cataaagcag acaattatgc agaagaagga aattataaag
     4981 gagtcgagca tatgcacttg cttgccggta tcggtctttc cttgatgctt ggtgttgtgg
     5041 taatggtttc ttatatggtg ggaagcaata cgatcggaaa tctattaaat atgattcctg
     5101 acttcgttca aaaggggttg tcagtagcta cagggttgat tccagctctt ggttttgcga
     5161 tgcttgctcg tttgctgatc aacaaacaag ttgcaccata ttttttctta ggttttgcta
     5221 tagcttctta cttagagatt cctgtgaccg gagtagctat ttttggtgcg attttagcag
     5281 ttgtggtagt caatattatg aatgtccgtc aactaccact tcaaacaact gcaggggagg
     5341 tagaagaaga tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat
     5401 cacgtatttt ggcgttcttt tcaaatggag ttttcttgga attatgaacg tcagatgaat
     5461 atggcttatg tttatgcaat gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa
     5521 atggcagcag ctttgaaacg gcatctagag ttttttaaca cgaccccaca tatcgttaca
     5581 ctgattttag gaatcacggc tgcaatggag gaagaaaata aggatgatcc tgaatttgat
     5641 gtgactgcga tcgatagtat caaaacatct ttgatgggac ctcttgcagg gttaggagat
     5701 tctttctttt ggggaacttt gcgattgatt gccacaggtg tgggtacctc tttagccatg
     5761 caaggaaata ttttaggccc tatcttgttc attttgattt tcaatattcc gcacattctg
     5821 tttcgctatc tagcaacagg ttggggatat cgcttaggaa cgggtttttt gaaaaagatc
     5881 caggcaaacg gtatgatgga aagtttgaca ttaggcgcgt ctattattgg actgatggtc
     5941 gttggagcaa tgactgccac gatgatcacc atcaatattc ctttaaaaat cggttcagga
     6001 gaaaatgctg tgactgttca aagtattttt gatgatatcg ttccaaatat cttatgtctt
     6061 ggagcatttg gcatagtgtt ttatctattg aagaaagaag tgaaaccatt aacgatcttg
     6121 atcggactag ctttctttgg aatttttggt tcgtggatag ggatttttta aaaaaggagc
     6181 agagaaaatg tttacgatgc aagattatat ctatgaagaa aaggaagtac taagtacgat
     6241 cttgaaaaag aatgattttt cgacaagaga acatatgaaa aaaacagtga atttactgat
     6301 cttagcaacg ggttcatctt ataacgcttg tttagcagcg aagccagcat tggaatctta
     6361 tggtgatctt actgtcgata ttcaagaacc tttttatttt ttcaattatg gaaaactttc
     6421 tccttcaatc gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga
     6481 agcagtaaaa atgatacaaa aacaagggtt acctgttgtt gctatcacaa atgacgtgca
     6541 aagtccactt gctttagaag ctgcacaaat tattgattta ggtgccgggg tagaatcagt
     6601 aggcttcgtt acaaaaggct attctgcgac tgttttacag ttactactct tagggatagg
     6661 aattggaata agtaaaaata aaatcagcaa aaaaatagaa caagactaca tgcaacagtt
     6721 gcgcaaaata atcaaccatt taccagcgat tattcaaaaa acggaagaat tttttgatgg
     6781 atatcaaagt ttatttcgtt tagcacagcg tttcgtcacc attggatatg gtccaaactg
     6841 gggaacagct aaagaagcag agacgaaatt gactgaaaca atccgcgttc cgtcacaagg
     6901 atttgagtta gaagcttata tgcacggccc atacctggag gcagatgctt cgcatttgct
     6961 attctttatt gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc aaagatatat
     7021 gagtcggtac gttggagaga ctctcaccat taccacaaaa aaagcaagaa acgagaaaac
     7081 acttggatta gcaatagaat gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt
     7141 tcaactattc gcttataaaa ccgcagtagc taaaggtatt gacttgaaca aaaagatttt
     7201 tgaagacttt gatacagtct tgaaaagtaa attataagag gtggaaatca tgctgaaatt
     7261 caatgaagaa gaacaaatca aagcaataaa aggcgcacta gctttacgtc cgcaagtaga
     7321 agaaatcatc gataaacttt atgcggaaaa atttgacgct gtctattatc taggaatcgg
     7381 tggaacgtat gcttcctcga tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc
     7441 tgtctttgta cagcatgctg cagaatatta cactactgga aacaaaagat taacaaaaga
     7501 ttctatcgtt attttgtcct ctgttaccgg tacaactcaa gaagtagtga aagcagtaga
     7561 acaaatcaag aaagtaggag cgacactgat tggatttatc gataaagcga acagtaagtt
     7621 gtctcagcta tgcgattttg tggtcactta tccagctcct ggaacagaac aaatcaaatt
     7681 ttttatggca gcagatcgat tgatgtattt gaatggagaa ttcgaagctt attcagaata
     7741 ctatgaacag ctcgaacgtt atttaccaac agggttagtg gaagcagaga aaaaagcaga
     7801 tgcatttggc ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt attttattgg
     7861 agcagggaat caatggggag ctgtttattc atatgccatg tgttactggg aagaacaaag
     7921 ctggcttcca tcgaaatcta tccatgctgc cgagtttctt cacgggacgt tagagatcgt
     7981 agaagaaaca accccagtca cattattttt aggcgaagat gaacagagag tgttatctga
     8041 acgtgtggct aaacttttgc caaaaatttg cagcaattat acattgatag acacaaaaga
     8101 ctatccagta gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt tcttattgat
     8161 gcatgtagtg acacaacgca tcgatgctca tgtggagcag ttgaactgcc atccattaga
     8221 gatacgccgc tactatcgtc agtttgatta ttaggataga ctggataaaa agaatataaa
     8281 aaataaagag agcagcgata actgctccct ttattttttt aaaatattgg catcaattac
     8341 gttataaaac acattttttg cattatctac agttcaactt ataatctgtt taatgggaca
     8401 tgtgggtgcc aaccattttt tgttatgcaa tagtactatt tagttgttgc atggatccgt
     8461 agtaaaagtc caagtacaac gagttgcgcc agtttacata gcgttttgta tccagaatgt
     8521 tgactaaaac aaaagttcct atgtcaaccg tcaacagaga caatctgtcc gttgcaggac
     8581 ctgacagatt tagaaatgtt caatctgtct cttatgtatt ttaaatcaat actcactagg
     8641 agaagtttcc taacctccta atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc
     8701 ttaggaacaa gcaaagcata acgagtgtaa aaataaaatg tagactattg taacatatca
     8761 ttgattattt caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa caatggaaat
     8821 tagtcccaac tttaagaaat gtatactata atggaaatta tcaattattt atttggagga
     8881 atatccatga aacaaaaaat cgcagttctt ggtcctggat cttggggcac tgctttagca
     8941 caaacattgg cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt
     9001 gatgaaatca ataagtatca tacaaatcag cattttttac ctgatttgac gatcccagaa
     9061 tcgattatcg gatataaaga attggcagaa gcagtggata acgcagatgc tattttattt
     9121 gttgttccaa caaaagcgat tcgttcagtc gcacaggaac ttattggtaa aatgaatact
     9181 aagccaatca ttatacatgc aagtaaagga ctagaacaag atacgcataa acgaatctca
     9241 gaagttttgg ctgaagaaat tccagaagaa aaaagacagg caattgttgt attatctggt
     9301 cctagccatg ctgaggaagt ggcagtacat gatatcacaa caattacagc tgcaagtatt
     9361 gatcaaaaag cagcagccta tgtacaaaaa ctgttcatga atgattattt caggatttat
     9421 acaaataatg atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt
     9481 ggtgccggcg ctatccatgg attaggattc ggagatgatg caaaagcagc aatcatgact
     9541 cgaggattgg cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt
     9601 atcggtttga gtggtgtggg ggacttgatc gttacttgta caagtgttca ttctcgcaat
     9661 tggcgtgccg gtaacttgtt aggacaggga cataagctgg aagaagttct agaaaacatg
     9721 ggaatggtcg ttgagggtgt ggcaacgaca aaagcagcag tagagctagc tcaacagcta
     9781 ggtgttgaga tgccaattac tcaaacaatc tataatgttt tgtataatgg agaagatatc
     9841 aaaaaagcag caaaagaaat catgttacgt gacggaaaaa tggagaatga attttctctt
     9901 cgttaaatag atgatcaata gttaatc
//
LOCUS       DARBEG010000095         9893 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000095 DARBEG010000000
VERSION     DARBEG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9893
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.096"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            205..684
                     /locus_tag="SCK20_002376"
     CDS             205..684
                     /locus_tag="SCK20_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146404.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            717..2945
                     /locus_tag="SCK20_002377"
     CDS             717..2945
                     /locus_tag="SCK20_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1146405.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3074..4717
                     /locus_tag="SCK20_002378"
     CDS             3074..4717
                     /locus_tag="SCK20_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1146406.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4790..5335)
                     /locus_tag="SCK20_002379"
     CDS             complement(4790..5335)
                     /locus_tag="SCK20_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG1146407.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5358..5843)
                     /locus_tag="SCK20_002380"
     CDS             complement(5358..5843)
                     /locus_tag="SCK20_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1146408.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5833..6645)
                     /locus_tag="SCK20_002381"
     CDS             complement(5833..6645)
                     /locus_tag="SCK20_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG1146409.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6784..8316)
                     /locus_tag="SCK20_002382"
     CDS             complement(6784..8316)
                     /locus_tag="SCK20_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG1146410.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            complement(8425..9456)
                     /locus_tag="SCK20_002383"
     CDS             complement(8425..9456)
                     /locus_tag="SCK20_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1146411.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT         3291 a         1834 c         1904 g         2864 t
ORIGIN      
        1 aaatattcca acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga
       61 gatggtgttt ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct
      121 ctataatgtc gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa
      181 ataaaaggat gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta
      241 atcgttgtag aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa
      301 gggctttcac tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag
      361 gtatggcgac atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt
      421 attttagatt tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt
      481 tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac
      541 cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat
      601 tacgatacac tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt
      661 cttttagatg agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg
      721 agaatttctc actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga
      781 agaaaccgaa aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag
      841 ggaattggca agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt
      901 cggggaagac acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag
      961 tgacctgttt tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag
     1021 attctaataa ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg
     1081 aagctgtact gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa
     1141 aattagcaga gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct
     1201 attacccttc tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc
     1261 cgactgtcat ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg
     1321 ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca
     1381 ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc
     1441 aacatgcgga aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga
     1501 aaactatgat ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg
     1561 atgaaactgc aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt
     1621 gggcaaatac ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt
     1681 ataaattatc cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg
     1741 gattgctact ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg
     1801 agcatacaaa agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa
     1861 atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg
     1921 agggagaaga gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa
     1981 aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt
     2041 ctcaagaaat gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga
     2101 ataaagaagc gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc
     2161 accgaccagc tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc
     2221 caatagtaga atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga
     2281 cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg
     2341 atttgagaaa ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg
     2401 tacggatcga tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag
     2461 cagcgattgc cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag
     2521 atacagcaga ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag
     2581 acggatttca atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg
     2641 atggtgctgc acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac
     2701 tgttgcttaa tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag
     2761 caaaagaaga tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct
     2821 ccgcagctat tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg
     2881 tagatattac tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc
     2941 tatgataaaa taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag
     3001 ttaccggtaa caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa
     3061 ggagtgaaac gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat
     3121 ctatcctgct agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg
     3181 tgaaaaactt aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta
     3241 tcaatctcca tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag
     3301 attcggaacg atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa
     3361 agtgattatg gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc
     3421 gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg
     3481 caaagaaccg aataattgga catcgatttt cggcggttct gcttgggagt attctcatga
     3541 aactggacag tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag
     3601 cgagaaattg aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga
     3661 tggatttcgt ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac
     3721 agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt
     3781 gaaacatgtt tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc
     3841 agaagaagga ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga
     3901 tcatatccat atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca
     3961 tgcattaagc gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga
     4021 aaaccatgat gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc
     4081 tatgtctgca aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta
     4141 tcaaggacag gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc
     4201 agtagattcg aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc
     4261 attggatatc attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc
     4321 ggaacaatat gctggttttt ctacacatga accttggctg atcactaatc caaataccaa
     4381 aacgatcaat gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa
     4441 catgatccgc attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct
     4501 tcatgaacat ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat
     4561 tatggtaaac ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc
     4621 ctggacatta ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc
     4681 accttatgaa gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta
     4741 gttggacttt gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg
     4801 ctctaggacc accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa
     4861 gggttttcaa acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg
     4921 tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga
     4981 actggtcaaa agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga
     5041 caatttcgaa attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat
     5101 gttcacaacc ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac
     5161 ggatgatcca ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt
     5221 cgcttgtgct gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata
     5281 tgtcattaac tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct
     5341 aaaaccttct ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt
     5401 ataggaaagg caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc
     5461 actccagccg gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact
     5521 aagcttccta ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca
     5581 gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct
     5641 gaaatcaaat cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca
     5701 taaagaaaag cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc
     5761 ggtacaggga tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag
     5821 atcgttaatt gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac
     5881 acttccggta tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt
     5941 actttctgct actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga
     6001 agaaaaggca caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac
     6061 ctcataatga tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc
     6121 gcctttgatc acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat
     6181 ttctgttaac gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg
     6241 cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt
     6301 gatcacagct tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg
     6361 ttttgttttt atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat
     6421 tttgatacca tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc
     6481 caaagatata gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa
     6541 ggcgcttagt ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc
     6601 accagcaaat ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat
     6661 gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct
     6721 catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa
     6781 tagttaggac ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt
     6841 agctgttcgc gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc
     6901 atggacttgc tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat
     6961 cggttgtgga gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga
     7021 atctaataaa aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg
     7081 ccgatctttc aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca
     7141 ccaatcgcca taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg
     7201 tcctttagac agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga
     7261 atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact
     7321 tgttttcaaa ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc
     7381 atatacttga agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt
     7441 ctggttgttt atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg
     7501 atgctcctta gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat
     7561 tggccggtga gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat
     7621 cataggaaat cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg
     7681 agagatgtga aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca
     7741 tttcgtccgc catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt
     7801 accagaagta tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga
     7861 caaagcaggt tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga
     7921 aatttctctc actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg
     7981 atcgataaat acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc
     8041 accaaggtaa gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat
     8101 aataactttg tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac
     8161 actttcgcag cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg
     8221 ctcatcaaag ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa
     8281 taagttcaaa ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa
     8341 gtaatataca aattcaagat aatcgtaaca taatccttta tataataaca tagtttattg
     8401 tgaaaaaaag atcatacatt tgttttatat ggaagaaacc cgaacgaatg ccgctaattc
     8461 gtgataagaa aaatcaacca tcttttccca gatatcttga aaagaatggg atgagaggta
     8521 tcgattacca cataaataat ttaccgcgtc agcatcatct aaatatagtt ctgcataata
     8581 acgatatgtt tttctgcata gttctggcga tacgttttta tatactgtaa ttttgctcaa
     8641 aatttggcga atagtccgag gagaaatttg ctgatcatgt gaggaacgaa aaacaaaaga
     8701 attttcgtcc gtattttgtg ctgttaactt aaaatagttc tccagaaatg gcatcgcttc
     8761 ttttggtatt ggtactttat tcagtctgtt ttcttttcta ttcagattaa gaatctgctt
     8821 ggtcaaagat acatctttac gtttaagttc tactacttca tggatcttta atccgcaata
     8881 cagaagaatg gcagtaattg caaggttcct cggtctattc ttttcttcaa agctagcttc
     8941 tctgcttgtt tgaaagcctt caatcgggat aaacaaatgc tcaaaatctt tgaaaagttc
     9001 tgcatcgata tatcgcggag ctgctttggt tgtttccggt aaacttgtac ccaaaagaga
     9061 aatctcttct tcaatcagtc ctttttgata caaaaatcgt aaaaaatgat ttaatgaata
     9121 ttgcttcctt gaaatcccgt aatagctatt ttcaaacaat tgctccgttt cttggttcgc
     9181 tgtagtttgg aacgaacgag tatgtcgata gattttagct aagaaattct gataatcttg
     9241 tttttctaaa tggataatca gctgatcatc cacttcttca gaaaatactt ttctcaaata
     9301 atcagtaaac agatgaaaat cttttgcgta ttcatagcga ctgcctaatg aaagggaaga
     9361 acgattttct tctaaaaact gttgaaagac ggaagggagc aagtgtgtac gttgctttaa
     9421 cttttcttgc agttgttttc tagttttgct gtccattaca aattccctcc aatagtgttc
     9481 attttctata ttttaccgca tttcatcaaa tgaagataga cagtttgtct tgatcttagg
     9541 aagcaaattt ttaactaagt actagctaaa agatatgaag taagaagata catttttagt
     9601 tattttatta gtgcagtcat tcttttgatc ttttaattga tttatcctct ttttagctat
     9661 gtggttactt catgagcaat gaaatggatt tggttcgttc taatggcagg aggctctttc
     9721 ttttatttcc gaaaaattag aaaacatttt ttcagaatcg attggctctc catttattca
     9781 ataatggttt gtctaacgat attgttggga taattcttaa agaagtatta gaaatagttg
     9841 gtacttcatt actttacatg aagatttatt ctatggtagg agcaattata ttg
//
LOCUS       DARBEG010000096         9830 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000096 DARBEG010000000
VERSION     DARBEG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.097"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..205)
                     /locus_tag="SCK20_002384"
     CDS             complement(<1..205)
                     /locus_tag="SCK20_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146412.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(221..835)
                     /locus_tag="SCK20_002385"
     CDS             complement(221..835)
                     /locus_tag="SCK20_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG1146413.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(982..1665)
                     /locus_tag="SCK20_002386"
     CDS             complement(982..1665)
                     /locus_tag="SCK20_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1146414.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1774..2232)
                     /locus_tag="SCK20_002387"
     CDS             complement(1774..2232)
                     /locus_tag="SCK20_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG1146415.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2316..4079)
                     /locus_tag="SCK20_002388"
     CDS             complement(2316..4079)
                     /locus_tag="SCK20_002388"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG1146416.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4300..5760)
                     /locus_tag="SCK20_002389"
     CDS             complement(4300..5760)
                     /locus_tag="SCK20_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146417.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="SCK20_002390"
     CDS             complement(5968..6894)
                     /gene="glsA"
                     /locus_tag="SCK20_002390"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG1146418.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6917..8344)
                     /locus_tag="SCK20_002391"
     CDS             complement(6917..8344)
                     /locus_tag="SCK20_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1146419.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8375..9589)
                     /locus_tag="SCK20_002392"
     CDS             complement(8375..9589)
                     /locus_tag="SCK20_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG1146420.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2917 a         2041 c         1628 g         3244 t
ORIGIN      
        1 atttgttaaa gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac
       61 catctaaagt aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact
      121 gcccttgcag aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg
      181 atggtttata gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg
      241 tgaaaatttc tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt
      301 ttcttggatc gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct
      361 gtacttctag caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt
      421 catataagga tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta
      481 ctgaatcagt taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg
      541 ctgctgtttt atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc
      601 ttgtctcaag aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca
      661 aggataagat gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca
      721 ctatatcttt tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa
      781 tcagcaactt gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt
      841 cgccttcttt ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg
      901 cttttttcat cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag
      961 aatctgtgcc gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt
     1021 agtgatcaat tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc
     1081 atatgctttc tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc
     1141 gacttgttct atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata
     1201 ttctgtatag cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag
     1261 cgcttcctca aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg
     1321 gacaagttca gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat
     1381 gatttctgct tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac
     1441 ttctgaccca tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt
     1501 gatttcattg atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc
     1561 agccaaagcc attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg
     1621 acaagaaacg acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt
     1681 aatgttatat aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa
     1741 taatgagcta tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc
     1801 gtatacgacc agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt
     1861 aaaaaactca gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt
     1921 ccaagcatgc tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat
     1981 gatacaaact ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa
     2041 aggctcattc tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt
     2101 tacaactagt tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc
     2161 taatttttct gattgataac taggatattt ttcttggaac tttttgacat attcttctac
     2221 ttgctgatac ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta
     2281 gtataaacaa ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga
     2341 ctatcttcaa cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga
     2401 acgctatccg tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca
     2461 taataacgac tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg
     2521 actaattcgt gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt
     2581 cctggattga tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt
     2641 cctaattcaa gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt
     2701 tgataggctg gaatactata atcatttgcg atagctgtac catgttctac accatgtgta
     2761 ggaattcgga ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc
     2821 atatcctctt ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga
     2881 atgtcaaatg ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa
     2941 ataatcactg gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc
     3001 aagaaagtac ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg
     3061 aaagtgatag agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct
     3121 tggaatgttt tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct
     3181 gtacttgttt gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca
     3241 gaagttactt gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc
     3301 attttattat cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca
     3361 atcggatgat caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt
     3421 ccattatcca cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta
     3481 ccgttggttt gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata
     3541 gacatctcat tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc
     3601 aatccttcaa aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt
     3661 cctgattctt tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta
     3721 gatgtgtgcc ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc
     3781 acttcacggt aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca
     3841 taagactgat gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga
     3901 aacgcaatcg ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg
     3961 tccttttgca agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat
     4021 aattttttga catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata
     4081 atacattctc tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct
     4141 attctactac ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc
     4201 ttgttatata agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa
     4261 ttaaaaagct ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa
     4321 taggtgaagt tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg
     4381 atagatcttt gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac
     4441 cactcttttt ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat
     4501 aggatttatt tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa
     4561 ttgtttgact gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc
     4621 actgttgatc aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag
     4681 ttggatttga tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag
     4741 ttgttctttt tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca
     4801 caacgtacgg acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat
     4861 ttcagcgata ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa
     4921 atttttataa acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt
     4981 tctactgttg aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc
     5041 tttttgctct tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt
     5101 cttatcttct tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc
     5161 tcgtatgata aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac
     5221 ataatcaatc aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc
     5281 ccaattgaca taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat
     5341 gatttcttta tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat
     5401 tgtatatact ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat
     5461 accattaaaa taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt
     5521 gagctcagaa agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac
     5581 ttgttctttt atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt
     5641 tggtgtagtc acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa
     5701 taattgttca cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat
     5761 tcctgataca cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat
     5821 ttttgtttcc tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga
     5881 caaaagtcaa acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac
     5941 ttttacccca gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac
     6001 tacatctttc aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct
     6061 gaagacacct atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct
     6121 ttttgcaggt acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt
     6181 tgtcataatt gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca
     6241 aggagcaatt cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac
     6301 attgagggag cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat
     6361 ttgattacct ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga
     6421 atgatagatt tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga
     6481 caagattcgc tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat
     6541 cgctcctgca ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat
     6601 gatagaattg aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat
     6661 tccgttgtct ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa
     6721 gcgcacatcg gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat
     6781 acctaattgt ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt
     6841 ttgataatag tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat
     6901 aaccagcttt cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg
     6961 taaatggaat aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat
     7021 gctgtccagc tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag
     7081 tcaaaagacc taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg
     7141 ctctttcaag actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt
     7201 acaacatcag gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc
     7261 ccccgccgct taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca
     7321 tcaaatgagc aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt
     7381 cttctgctgc tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga
     7441 tgacacctaa acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa
     7501 tgtgtcttaa taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt
     7561 gaggtactgt agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa
     7621 ccaatacaaa cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac
     7681 tggcagatgt ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag
     7741 gcaatttcga aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc
     7801 ctgaagcgac ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag
     7861 ctacaaaacc gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta
     7921 aaatagccca gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag
     7981 caccaatacc cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc
     8041 caactgttat ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg
     8101 tgcgactgac ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg
     8161 ctgcgcacaa tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta
     8221 agctgaagcc tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg
     8281 tcatagccaa aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt
     8341 gcatgaatct tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga
     8401 aaaagtccaa tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg
     8461 ccgattttaa aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa
     8521 tcacttgaat ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg
     8581 caaagatccc tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat
     8641 caagtgcatc atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac
     8701 ccgcaatgat cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa
     8761 tggctgccaa tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt
     8821 ttacttttgc aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac
     8881 caaatgcatt gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc
     8941 agccaaacca aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc
     9001 tttctcctaa aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc
     9061 cagctgtcgt gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa
     9121 atccgccacc ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa
     9181 caagtataat atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa
     9241 cggtaatcac gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac
     9301 catgaaacat gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc
     9361 caataacacc gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa
     9421 tcgttgttat cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc
     9481 gtgctaatcc gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac
     9541 ataaaatcat gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct
     9601 cctttctacg attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga
     9661 attaatactt gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt
     9721 tttcgttttt cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt
     9781 ttattttcgt ctttttttat ttttaaagat attttttcat aaggaataaa
//
LOCUS       DARBEG010000097         9513 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000097 DARBEG010000000
VERSION     DARBEG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.098"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            11..538
                     /locus_tag="SCK20_002393"
     CDS             11..538
                     /locus_tag="SCK20_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG1146421.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            601..1971
                     /gene="radA"
                     /locus_tag="SCK20_002394"
     CDS             601..1971
                     /gene="radA"
                     /locus_tag="SCK20_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG1146422.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            2005..3162
                     /locus_tag="SCK20_002395"
     CDS             2005..3162
                     /locus_tag="SCK20_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG1146423.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            3332..4792
                     /locus_tag="SCK20_002396"
     CDS             3332..4792
                     /locus_tag="SCK20_002396"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG1146424.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     misc_binding    4789..5028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5114..5650
                     /gene="cysE"
                     /locus_tag="SCK20_002397"
     CDS             5114..5650
                     /gene="cysE"
                     /locus_tag="SCK20_002397"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG1146425.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            5647..7059
                     /locus_tag="SCK20_002398"
     CDS             5647..7059
                     /locus_tag="SCK20_002398"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG1146426.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            7056..7451
                     /locus_tag="SCK20_002399"
     CDS             7056..7451
                     /locus_tag="SCK20_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG1146427.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            7444..8304
                     /gene="rlmB"
                     /locus_tag="SCK20_002400"
     CDS             7444..8304
                     /gene="rlmB"
                     /locus_tag="SCK20_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG1146428.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            8308..8838
                     /locus_tag="SCK20_002401"
     CDS             8308..8838
                     /locus_tag="SCK20_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG1146429.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            8915..9493
                     /locus_tag="SCK20_002402"
     CDS             8915..9493
                     /locus_tag="SCK20_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1146430.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
BASE COUNT         3255 a         1729 c         2142 g         2387 t
ORIGIN      
        1 aggaaaaccg gtgagaaaaa gaggatttga agttatttct aaatataaag atcaaaatat
       61 caatctaccg cagcgcgcta cacatcatgc tgctggctat gattttgaag cagcagaaga
      121 ggtcgtgatt ccaagtatct ggaaagcagg ctggaaaaag gggttggaaa cagtcgcaga
      181 tggattgaaa aaagatgtat cagaaaatga aaaacaattg aaaccaaccc tcgtgcctac
      241 tggaatcaaa gcttacatgg gagaagatga atttttgcag cttgctaacc gttcaagcaa
      301 tccgttgaag cggtttttag tgttgacaaa cggagtaggt gtgattgaca gtgattatta
      361 caataatgag gcaaatgaag gccatatcat gttccaattc accaactttg gtttcaagga
      421 tgtagtgatc aaaaaaggcg agcgtatcgg ccaagggatc tttttgccgt ttttaaaagc
      481 agatcaagat caaacgactg ctcaacgtca aggcggattt ggttcttcca gtaaataaaa
      541 cgttaaagat acttaagctt gatttaatgt ctcatgatta ggataaaagg aggcggtaca
      601 atggcaaaaa aatcaaaagt acaatttgtt tgccaaaatt gcgggtatgt gtcgcctaag
      661 ttcttgggac gctgcccaaa ttgcggaaaa tggaatacga tggtcgaaga aatcgaacaa
      721 gatacaacgg accgcagaac tcggacaagt ttgacaggag aaaaagcaaa accaacaaaa
      781 atcgcagacg tcgtccctaa aaaagaacct cggatcaaaa caaaattaga agaactgaac
      841 cgtgtgttag gcggaggtgt ggtgccaggg tctatggtct tgattggtgg cgatccaggt
      901 atcgggaaat ccactttgct gttacaagtt tctcagcaat tagcagccat aggcggaaaa
      961 gtcttatatg tatccggcga agaaagcgca gagcagataa aattacgcgc ggaacgcctc
     1021 ggaagtatca acacagaatt ttatctatac gctgaaacag atatgaacga aatcagtcgt
     1081 gccatcgaac atatctcccc tgattatgtt atcattgatt ccatccaaac gatgacgcag
     1141 ccagacatca caagtgtggc aggaagtgtc agccaagtaa gagaaaccac tgcagaacta
     1201 ctcaaaaccg ctaaaacaaa tgggatagcc atttttatcg tcggtcatgt taccaaagaa
     1261 ggctcgatcg ccggcccaag aatgttagaa catatggtgg atacggtgtt atattttgaa
     1321 ggtgaacaac atcatagttt tcggattttg cgagcagtaa aaaaccgatt tggctcaacg
     1381 aatgagattg ggatttttga aatgcatgag catggattgg aagaagtcgc taatccttca
     1441 cagattttct tagaagaacg cctagacggt gcaactggct ctgctatcgt tgtggcaatg
     1501 gaagggacac gtccaatttt ggtagagatc caagcattag tcacacctac tatgttcgga
     1561 aatgccaaac gaacaacaac aggtcttgat ttcaatcggg tatctctgat catggcagtg
     1621 ctggaaaaaa gagctgggtt gcttcttcag aatcaagatg cttacttaaa agctgcaggt
     1681 ggggtaaaat taaatgaacc agcgattgat ttagctatcg ctgtcagtat tgcttcaagc
     1741 tataaagaaa aaggaacaca gccaacggaa tgtttcatcg gtgaaattgg actgactgga
     1801 gaaatcagac gtgtcagtca tattgaacaa cgagtcaaag aagtccaaaa actcggcttc
     1861 acaaaagtat acctacctaa aaacaactta ggtaactggg aagcgccaaa aggaatcgag
     1921 atagttggag tagcaacttt ggctgaaacg ttgaagcgtg tgttccgcta actaataaat
     1981 gattgtcgaa acggaggaaa aacgatgcaa aaacgtgtca ttactttgct gatgatcatt
     2041 gtcggcgcaa gcttgggtat ttcattttta ccctcagctt ggcaggcaat cggtcaacag
     2101 tcaaacggat ggctgaataa tacatttacc aacagcctgt taggcgcact tattttcttt
     2161 ttattatctt tgctgttggc caaacatatc tcagctatta tcaaacaagt agaacaaaaa
     2221 ctaagtgagg tcagcttgac ttatcttctc ttcggtgcga tcggtgcgat catcggattg
     2281 actttaggtg ttgtgatctc gacccctctt tacaatatgg aaattccatt cgtcaatagt
     2341 gtattgccga ttttactgat gatcatctta ggctatctgg gattgagaat gggaacgaca
     2401 cgaatcgaag aatggaagaa agtttttggt tcacgaagca aaaaaatcga gcaagaaaca
     2461 gctagtcaag ttgagagtca actgttagaa cgaaaatcag gagaaaattt ccataaatat
     2521 aaaatcttgg atacaagcgt gatcattgac ggtcggattt atgatattac aaagactgga
     2581 ttcttagaag gaaccttgat gatccctaat tttgttcttt acgaactaca atacatcgcg
     2641 gactcagggg atagtttgaa acgtgtgcgt ggtcgtcgcg gattagatat cctaaatgcc
     2701 ttgcaaaaag aagagggtat atctgtcgaa atgtacgatg gagactttga agatatcagt
     2761 gaagtagaca gcaaattgat caaattggct aagcttttag atggtgtcat tgtaaccaat
     2821 gactataact tgaataaagt ctctgaattc caaaatgtac ctgttcttaa tatcaatgcg
     2881 ttggcaaatg ccgtaaaacc agtcgtcatc cctggtgaaa cgatgaatgt tctagtagta
     2941 aaagccggga cagaacgcca acaaggagta gcctaccttg atgacggcac gatggttgtc
     3001 gtagaagatg gacagcatta catgaatgaa cgtatcgaag tcgttgtaac aagtgccctt
     3061 caaacagctg ctggacgcat gatctttgct aaacctgcac atgcaggacg aggaatcgac
     3121 aataaaaaag agacagagaa aaagactaaa caatcaaaat aaaaaagaaa ttcagccaac
     3181 tggtaaggaa attcaaccag ttggctttac tatttacctt agaaaaggta aaatttgtgc
     3241 tgagtggctt taaataaatg atatttgaga agccgtaact gacggaattt ttgctgttag
     3301 tcaaagtcta gtttgaaaga agaggaagaa aatgacgaaa gtacgcgtac gctatgcacc
     3361 aagtccaact gggcacctgc atattggaaa tgcaagaact gcattattca attatttgtt
     3421 tgcccgccac aatgatggag actttattat tcgtattgag gatactgacc aaaaacgtaa
     3481 tatcgaagat ggcgaaaaaa gccagttgga aaaccttgct tggttgggaa tgaattggga
     3541 tgaatctcct gaaaatcctg gagaatacgg cccttaccgt caatctgaac gtaaagaaat
     3601 ctatcaaccg ctgattgatc agctgcttgc tagcaatcga gcatacaaat gctattgcac
     3661 agaagaagaa ttagaagcag aacgggaagc acaacgcgca agaggagaaa tgcctcatta
     3721 cgcaggaaca tgtgcgaact tgacacctga agaacaagca gaaaaagaag cacaaggtct
     3781 agaatctgtt gtacgtttcc gtgtgcctcg caatacagaa tacgcattta cagatatggt
     3841 gaaaggcgct atttcgttcg aatccgataa tatcggtggc gactttgtca tccaaaaacg
     3901 cgatggtatg ccaacttata attttgctgt tgcggtcgat gatcatttga tgaaaatcac
     3961 tcacgtttta cgtggagatg accatattgc caatacacca aaacaactga tgatctatga
     4021 agcatttggc tggacaccac caaccttcgg acatatgaca ttgatcatca acagtgaaac
     4081 aggtaaaaaa ctaagcaaac gtgacgaatc aatcttgcaa tttatcgaac aatatcgcga
     4141 attaggctat ctaccagatg caatgttcaa ctttattgca ttattgggct ggtcacctgt
     4201 gggcgaagac gaaatcttcg aacaccaaga attgatcaaa atgttcgatc caaaacgcct
     4261 aagtaaatca cccgcagctt tcgatgcgaa aaaactgcaa tgggtaaaca accattacat
     4321 caaagcaatg gatctagatg catttactga catgtgtctg ccttatctaa tcgctgatgg
     4381 acgtgtagca gaaaacccag atgaaaagac aatcgaatgg gtgaagaaga tcgttagcct
     4441 gtatcaacct caaatgagct atgctgcaga aatcgttgat ctgtctacgc tatttttcaa
     4501 tgaacatcct gtattgaatg aagcagcaaa agaagtactt gctggtgaaa cagttcctac
     4561 tgttctccac gcattcaaag cacagctaga acaaatggaa gtagtagatg ctccaactgt
     4621 caaagctgcc atcaaagctg ttcaaaaaga aactggcgta aaaggaaaaa atcttttcat
     4681 gccaatccgt gtagctgtat caggacaaat gcatggtcca gaattgcctg aaacgatcga
     4741 attgcttggt cgtgaaaaat cattagcaca tttaaatcaa gtactgaact aaacacgatg
     4801 aacagaaaaa agtaagcagc tgatcattac aagagagata ccgggtggtg caaggtatca
     4861 atgaatggtt gccgaaatgc ctctgggagt ggtcttggcg aaacacagaa aacaggaaaa
     4921 actgttttat aggaaacaaa gtagccaaga acgtataaca ggcgttagct gtcagagata
     4981 atcattgatt atgaagaaaa gtggaaccac gcggaaagcg tcttttgata aggaaactta
     5041 tttaaaaggc gcttcttttc tttataagaa gatgatttcc tacataaaat tcttacgaaa
     5101 ggagtggaaa gtcatgagtt ggtttacccg agcgattgat gcagcaaaga gaaacgatcc
     5161 ggctgcacgt tcgacagctg agatcgtttt gacataccct ggttttcatg ccttgttttg
     5221 gcatcgtttc tctcattttt tatatcagca ccggctcttt ttattggcga agatcaatgc
     5281 tcaattctgg cgtttcctaa ctggtgttga gatccatcca ggagcaacga ttggaactgg
     5341 cgtgttcatc gaccatggaa tgggaatcgt cattggcgaa accgcagaaa tcgaggacga
     5401 tgtgatttta ttccatggcg taacattagg cggcacagga aaagaaacgg gtaaaagaca
     5461 cccaactgtt aaacaaggag ccatgctctc tgcgaatgcg cagatattag gaccggtaac
     5521 gatcggaaaa aacgccaaaa tcggtgcagg tgccgttgtg ttgaaagatg ttccagacga
     5581 cgctacagcg gtaggagtac cagcaaaagt cgttcgaatc aaaggagaaa aagtgaggag
     5641 atcagaatga taaaaatcta caatacattg accagaaaaa aagaagaatt catccctata
     5701 caaaagggac atgtcggtat gtatgtctgc ggaccgactg tgtataatta tatccatatc
     5761 ggaaatgctc gtagcaccat tgctttcgat acaattcgac gctattttga atatcgaggc
     5821 tataaagtcg atttcgtatc gaatttcacg gatgtagacg ataaaatcat ccgtacagca
     5881 aacgaattag gtattacagc accagaagtt gctgatcgtt tcatccgagc atttgaagaa
     5941 gatacgaaag cgttgaatgt cgaacctgct acactgcatc caagagtcat ggatcatatg
     6001 ccagatattt tagcatttat tcaagtgttg gttgaaaaag gctatgctta tgaatcttca
     6061 ggtgatgtgt attaccgtac ccgtaaattt gaaaattatg ggaaattaag tgaccaatcc
     6121 atcgatgaac tggaagtcgg agcaagtcag cgtacagggg aagaacagca gcaaaaagaa
     6181 gacccattag atttcgcttt atggaaacaa gcaaaacctg gcgagatttc ttgggattca
     6241 ccatggggca aaggacgtcc cggttggcat attgaatgtt ccgttatggc aacaaaacat
     6301 ttaggagaaa cgatcgatat ccatggtggc gggcaagatt tagaatttcc tcatcatgaa
     6361 aatgaaatcg cacaaagtga agcgaaaaca gggaaaaaat tcgctaatta ctggatgcac
     6421 aatggctatg taacgattgg cgaagacgat gaaaaaatga gtaaatctct agggaatttc
     6481 atcactgtac atgaaatgat caaaaacgtt gatccacaag tcttgcgttt cttcatggcg
     6541 accacacaat accgtcgtcc gatccgctac agtgaaacga cgatgaaaga agcagccacc
     6601 aatttacaac gactgaagaa tacgtttgac aatttgaaat tccgccaaga aactgcctca
     6661 gagaatttgg cagatgatgc ggataaaatc gctttgttag aaaatttaga acagcgattc
     6721 atcgaagaaa tggacgatga ttttaatgca gcaaatggga tcacagtggt ctatgagctt
     6781 gctaagtggc tgaacactta tctagaccag ccgaaagttt ctgccgctgt cttggcacaa
     6841 ggagaagctt tattctcgca atggctttcg atcttcggta ttttatttac agcagaagag
     6901 ttattagatg aagagattga acaattgatc gaagaacgaa atcaagcaag aagcaatcgt
     6961 gattttgtcc gaagcgacga aattcgtgat ttgttgaaag aaaaagggat cttattagaa
     7021 gatacaccgc aaggaacccg atggagaaga gaagcatgag agattacaca caattgaacg
     7081 gtttagcctt ggcttatgtt ggagatgcga tttacgaaat ctatgttcgt gaccacctga
     7141 ttgatcaagg agaaacaaaa cccaataagc tgcatcgtgc ggcaacgaat tatgtttctg
     7201 cgaatgcaca agcctttttg attcagcaaa tgctggaaga acatcttttg acagaagaag
     7261 aagaacttta ttatcgcaga ggaagaaatg ctaaaagcca tacgagcgca aaaaatgctg
     7321 atgttacgac gtatcgggtc gcaacagggt tcgaagcgct catgggctat ctgcatttga
     7381 caaaacaaac cgagcggatg gaagaattga tccgctggtg tattcagaaa gttggtgaaa
     7441 aaaatgggta aagaaagaac aaacaaacat caatcgaaac gtggatttga agacaaaaaa
     7501 caaaatagat attcaaataa aaagcaaact ggacgaaatc agtcagtttc tgatcaaaat
     7561 gaagaagcat tagaagataa tttcgtcttt gggcaccatg ccgcagtaga agcgctacaa
     7621 caagggcgag ggaataaact ctttcttcaa gaagattcac gaggagaaaa agtagaagaa
     7681 ctgaaagaaa tcgcacgtga acaagctgtg ccagtcaaat gggttcctaa acaaaaatta
     7741 gaaactttga gcgatcacgg cgtgcatcaa ggagtcgttc ttgcgattac accttatgaa
     7801 tatttatccc ttgaccaact gattgaacga acaaaagaga aaaaagaaga ccccttcttt
     7861 ttgattttgg atagcatcga agatccacac aattttgggt cgattttacg gacagcagac
     7921 gcaagtggcg tcgatggtgt gatcatacca aaacaccgag cagttggaat cacacctatc
     7981 gtaacaaaga cttccactgg ggcagtcgaa catgtccctg ttgcacgcgt gacgaatttg
     8041 gtacaagctg tgcaacagct caaagaaaca ggattttggg tatttggcac agatatgacc
     8101 ggcacaaact tccaaaaatg gaatgccaaa ggatcaattg ctttagtcat tggtaatgaa
     8161 ggcaaaggaa tgagtccagg attgaaaaaa gaaatggatg aattgctgac gatcccgatg
     8221 accgggcacg tccagagctt aaatgccagt gttgctgctg gtttgcttat gtatcaggct
     8281 ttttcccaac gtcaggggga gtagaccatg aaaaaacagc tgttgattgt agacggctac
     8341 aacatgattg gttcgtggcc ggaacttgtc cagcttaaaa agcaggacaa aatggcggat
     8401 gcaagagaag ctcttttaca tcgtttatca aattatgcga aatatgaagg aatggaagtc
     8461 atcgttgtct tcgatgccca actagttccg ggcatccaac aaaattataa gaaataccag
     8521 ttggatgttg tttttacgaa agaagacgaa acggctgaca gttatatcga gagaatcgct
     8581 ggtcagctga atgaccgttt gacacaagtg acagtggcga caagcgattt agcagaacag
     8641 tggatgatct tttcccaagg agcattacga acttctgctt atgaattata taagacgata
     8701 aaaaaaacgg aaaaaacaat agcccaagaa aataaagata tgcattttat tgatttccga
     8761 cgtaattctc catggaatga agagcagtta aaaaaattgt cccaaaaatt ggacgattta
     8821 atgagaaaaa ataaataaca tatcgttcta atcagttcga tgaatcattg atgataagat
     8881 tcctccataa acgaatttat ggaggaatta ttttatgtct gaggagttat tgcgcgctgc
     8941 acgtaatggt gacgaagaag catttgctgc cctgtatcaa aaatattttc cacttttact
     9001 acgtttgaaa aacacctatt atttaagaaa ttatgtttct gaggattggg aacaagaagc
     9061 aaggatcgct ttatatcagg cattgagaac ttacgaaaaa agcaaaggtg ttagctttgg
     9121 aagttattat aaaagcgtta taagcaatca aatctattct ctgttgagga agcagaacgc
     9181 gctgaagcgt cgtgatcaaa aagatgaagt ctcaatcgac caaaaaatag agacagaagg
     9241 accagacttt ttagcagaga tcacattgca agaaccttta gcaattcagc atcttttatt
     9301 aaaagaagca atcgaaaatt gcgaaatcca gttttcaaaa atagaagcta aaatttttca
     9361 ttattatatg caaggcgttg attttgtaga catggctgaa tgtttagcaa tggatcacaa
     9421 gcaagtaatg agcgcttacg ggagagtaaa actcaaaata aaaaaacact tgaaatttta
     9481 taatgaggaa taatttagaa aaatcttaat gag
//
LOCUS       DARBEG010000098         9361 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000098 DARBEG010000000
VERSION     DARBEG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.099"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(92..760)
                     /locus_tag="SCK20_002403"
     CDS             complement(92..760)
                     /locus_tag="SCK20_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1146431.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="SCK20_002404"
     CDS             894..1181
                     /locus_tag="SCK20_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146432.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="SCK20_002405"
     CDS             1597..3135
                     /locus_tag="SCK20_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG1146433.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="SCK20_002406"
     CDS             3132..4097
                     /locus_tag="SCK20_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1146434.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="SCK20_002407"
     CDS             4084..5406
                     /locus_tag="SCK20_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1146435.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="SCK20_002408"
     CDS             5647..6390
                     /locus_tag="SCK20_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1146436.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="SCK20_002409"
     CDS             6405..7397
                     /locus_tag="SCK20_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1146437.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="SCK20_002410"
     CDS             7409..8146
                     /locus_tag="SCK20_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1146438.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="SCK20_002411"
     CDS             8136..8858
                     /locus_tag="SCK20_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1146439.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="SCK20_002412"
     CDS             complement(8942..>9361)
                     /locus_tag="SCK20_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1146440.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DARBEG010000099         8800 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000099 DARBEG010000000
VERSION     DARBEG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.100"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..778
                     /gene="menA"
                     /locus_tag="SCK20_002413"
     CDS             <1..778
                     /gene="menA"
                     /locus_tag="SCK20_002413"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1146441.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1592..2938)
                     /gene="gor"
                     /locus_tag="SCK20_002414"
     CDS             complement(1592..2938)
                     /gene="gor"
                     /locus_tag="SCK20_002414"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG1146442.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3072..4325)
                     /locus_tag="SCK20_002415"
     CDS             complement(3072..4325)
                     /locus_tag="SCK20_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1146443.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4331..5254)
                     /locus_tag="SCK20_002416"
     CDS             complement(4331..5254)
                     /locus_tag="SCK20_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1146444.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5475..7967)
                     /locus_tag="SCK20_002417"
     CDS             complement(5475..7967)
                     /locus_tag="SCK20_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1146445.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7974..8441)
                     /locus_tag="SCK20_002418"
     CDS             complement(7974..8441)
                     /locus_tag="SCK20_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG1146446.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8577..8650)
                     /locus_tag="SCK20_002419"
     tRNA            complement(8577..8650)
                     /locus_tag="SCK20_002419"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8614..8616),aa:Arg,seq:acg)
     gene            complement(8708..8781)
                     /locus_tag="SCK20_002420"
     tRNA            complement(8708..8781)
                     /locus_tag="SCK20_002420"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8745..8747),aa:Pro,seq:tgg)
BASE COUNT         2441 a         1867 c         1461 g         3031 t
ORIGIN      
        1 taattacatg gattatgaaa aggctcattc tgaagtatat aagtatgaag aaaatgtcat
       61 tggccgcaga aatctatctc ctgtattgat tcgaaacatg attttagcta tgattgcatt
      121 tgtttttgtt atcggcgtta ttctaacgat caatacgggt tggcttttac ttttgatggg
      181 atttatctgt tgttttattg gagtattcta tacattcgga ccgattcctt tatctcgaat
      241 gcctttaggg gaaattttca gtggggtgac tatgggcctt ggaatttttg ccatggtcgt
      301 ttatgtcaat acattcggtt cagatgtcta tgatctgaca gtcaactttt catctggaca
      361 atttgatttg gtaggaaatc tttgggctat agcagcaatc gtattagctt ccttaccctt
      421 ggtgtttacg attgccaata tcatgttggc taataatttg cgggatcttg accgtgatat
      481 tgaaaatcat cgctatacac tagtttttta tatcggacgt cctgcgggaa cagtcttatt
      541 ccagctattg atgtatgctt gctatttcgt gattttaatg gggttgttga gcggtatcta
      601 tgaatggccg atcttggtca ccttcttgac cttgccaatt gtgaagaagc atttggatca
      661 attcaagcaa acgttacccc agcctatcag tttcagctat tccatcaaaa atatggtctt
      721 attcaatgcc agttatgtat tggggctatt actaacaatc attatgcaag ccatttaaca
      781 gcatgaagag gttgtgaaaa aactgccctg catcaagtaa taagaacagc caaataaaaa
      841 tagctcctta tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
      901 cgtttatcag gaggttgtga aaaagtcgct tagtttcgag tattaagaaa aattttgtga
      961 aaatggcttt ccacattttt gcataaattt ggcttaatac cgagaaactg gcttttgaac
     1021 accgtttatc agaggttgta aaatcagtgt tttgtcctga gtattagaga aacaaatgga
     1081 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
     1141 actatttgtt cggaaaaagt gactaactat gacacaattt ttttcacagt cccttttttc
     1201 acttgatttg attcgtacca gcgaacttga cgagaacttt cttggtttgg taactatatg
     1261 gtattctttt aacatcctcg tttgcttctt ctaatttatt tatacaaagt cacacttttt
     1321 taaacatctg ttcttcgata catttctatt tcttttaaac atcgatacca atattttgaa
     1381 cttaagagtg cataccaaat ggattcattt actcttcgtc agttacgaag ataatcaaaa
     1441 tctccaactt ccctacttat ttcgttttga gaattatcga agcaatctta cataaataac
     1501 taaaaaacaa aaaaccagaa aatcagtata ctgattttct ggttttacat ggtttgttca
     1561 ataaaaagct gttggaactg ttaatattaa attatcgcat cgttacgaat tcttccgctc
     1621 ctgttggatg gatggctact gtattgtcaa agtcttcttt agttgcaccc atcttgatag
     1681 ccacggcaaa accttgcagc atctcatcga caccaacacc aatagcatgt aagccgacga
     1741 ttctttcttc ttttccgaca cagatcaatt tcatttcgca tttttggcga taatcattta
     1801 atgcaaagta cattggggta aagcgtgaac gataaacttt caattcattt tcgccatatt
     1861 tttccaatgc ttcttcttct gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga
     1921 ctgtcggaac aagtttatag tcaagataag aatctttctt tccattaaat aaacgttctg
     1981 ataaacgtct accagctgcg attgcaacag gagtcagatc taatttaccg atcacatcac
     2041 caacagcaaa aatatgttca tccgttgtat tttggtattt atctactttg ataaaccctt
     2101 ttttgtccaa ttccacattt gtattttcca gacctaattt ttcggtattc ggtacacgtc
     2161 caccggcaaa aagtacacaa tcaccagtta aggtttcacc attttcaaat agcaccgtat
     2221 attcgtcatt gtttttagta atttctttag caatatgatg tgcgtaagtt tttattccag
     2281 cttcttggta catttggatc aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc
     2341 gttcttttcg atatgcccac atgacttcac tacctaaacc acgaaataca cctgaaagtt
     2401 cagccgcaat gtatccagca cctaaaacga ttgctctctt aggtaactct gttaattcaa
     2461 aaaatccatt tgaatcaatt gcgtattctc caccaggaat ggtcatttta cttgcacggc
     2521 ctccagtagc gatcaagatt ttaggagcac gataggtttc accgtttact tcgatcgttt
     2581 gactatcgat aaaagtagcg taacctttta tagcctcgac atgattgcta tctaatcctc
     2641 tttgataagc accgtgtaga aaatcaatat atttttctcg tctttctact agaccggcga
     2701 aatcaacatt ttttaaatct gcttggatac cgtaacttga ggcatctcgt tcaattgttt
     2761 ccaaaatcgt acttgcttgc cacataactt ttttaggtac acaaccgacg ttgacacatg
     2821 ttccgcccaa ttcattcgct tcaatcagca aaacttttgc gccatgcatc cctgctctgt
     2881 ttgctgaagc aataccgcca gagccgccac caattacgat gtaatcatat atttgcattt
     2941 tgttcctcct tattgaagag atcaatcaaa acctttattt aacgatcctt tttctacaga
     3001 aaacaagtgt aacacttttc ttgaattttg tatgcgaaga tgcttaaaac agggatatat
     3061 cagctttttc cttatttacg ttttcggatc atatctccag gtttcaccgg ctgtgcgact
     3121 ggcatagtta aaatcatcat tggatttggt gctcggtcaa tggattcgcc ttcttcatta
     3181 tgcataactt caacggtttg actgaaatga ctgaatcctg gaccataaaa ttcgatttca
     3241 tctcctacag aaaacaagtt tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa
     3301 gataatactt caccgacaaa tttatatact gggattttac gacgttcacc gaataattgt
     3361 tcgttttcag ttggtgtatt gtagtaaaaa cctgtagcta attctcgttg tgctactttc
     3421 cataactcgt cgatccattc ttgcttgcag acatagtttt caggatcttc catataactg
     3481 tctacagccg ctttatagac atttgacacc gtagatacat aatggatcga tttcattctt
     3541 ccttcgatct tgaagctgtc tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt
     3601 gacatatcca ctgcactcat cgagaattct tcttccactt cgcctgtatc agttaaactg
     3661 tttcgttctt gaccaaaagg catatcgaac aatccatact tccagcgaca agattgagaa
     3721 catccgccac ggtttgcatc ccgcatcgac atatggttgg acaatgtgca tcgaccagaa
     3781 taagagatac acatggcacc atggataaat gcttcgattt cggtatccgt atttttacga
     3841 atctctgcca cttcctccat tgaaacttca cgagctaaaa cgacacgttc caacccttct
     3901 tctttccaga attccaatgt ctcaaaatta gttgctgaag cttgagtgga taaatggatc
     3961 ggcagacctg gggcttctgt cgcacaaatt tcgatcaaag ctggatcaga aacaatcact
     4021 gcagaaatac cgacatcacg caaactgcgg aaaaattccc ctgctccatc ttcatttcct
     4081 tcatgggtga ccatattagc agcaacgtat accttcgcat catgttcttt tgcaaaagca
     4141 actccttcag ccatttcttc ataagtaaaa ttacctgcac ggcttcttaa accatatgca
     4201 ttcccgccaa tatatactgc atctgctcca taatggatcg cggtttttaa cttttccagt
     4261 gttcctgctg gcgctaggat ttctggtcgt ttcaattttc tttttactgt catttctttc
     4321 atcatactcc ttatttgata gcagaaggat caatataaaa gaatcctgta tctaattcac
     4381 ggttttcagg atgcaattta ctgatttcgc tagtcatttt ttccgctttt tcttgatccc
     4441 agctaccttc taaaatttgt ttttttgctt cggcgaatag tcgagtgatg tttacgaatt
     4501 tttcgcctgg agtaaataat ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt
     4561 cattcaattc attcgagaga ttcagatcat tgctggcaaa aatatgtgtg ccatgactat
     4621 cttcataaat agaataatgt gtctcttctt ttttaggttc cgagataaaa agacctcttt
     4681 cacgatcttt ttgttcgtct tgtttcgtat agttatagta attctgtagc agtggacgtt
     4741 tggattgatg gatgcatgta gcaccataca caagggtttc tacaggaata tcgagttttt
     4801 cttccatcgc cttcatctct tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc
     4861 cttttttcgc ccagaaattg atttgacgtg cgctcgttac taatgtttca gcatcataaa
     4921 tatacggtat acgcaattca ggattttttt tcatcgtata aataatacct gtatcaccaa
     4981 ctgtgatcac atctacgttt atttctttta aaaaagccaa atattcaggt acatttttca
     5041 ttttttcagg atgaatgatc ccatttacag caatattcgc ttttttacca tacttgtgtg
     5101 ctaattctac caattgtcgc tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa
     5161 attgctcttc accgaaatat agtgtatctg cacctgcagc aagcaactgt tctgcttgtt
     5221 caatagtttc tactgtggtt atgagttcga tcataatttt ctcctcctac aacttcataa
     5281 tagtagcata gctctaaaaa ctttccaata cttcttctat ttaaagtcgg atttttgaaa
     5341 attatttcac aaaaaatcaa aataaagttc gatatattct gtccaaatat aaaaagagat
     5401 tgtgacaaaa atcttgtcac aacctcttaa tcaagtctta ctattataaa aattcagcta
     5461 cacaggccaa gtaactatag ggaacctact gtttcctttt taggtccgcg aacattcaat
     5521 gtgatcttac cttttgaagc acctactgtt acgtggtcac ctaaacgaat ttctccgcca
     5581 agcaaagcct cgctcaatcg atcttcaatt tctttttgaa gtgcgcgacg gattggacgt
     5641 gcaccatatt caggatcaaa tccagctttc ccgatcacat cgattgcaga tggtgtgatt
     5701 ttcacttgaa tatcttgttc tttcaatcgt ttgataatcg ctttgctcat gatttggacg
     5761 attgtatgga tttcatcttt actcaatgaa tggaagacga cagtctcatc gattcgattc
     5821 aagaattcag gtcggaaagc tttctttaac tcttctaaga tacgtttttg catggctttg
     5881 tgatctttag taacatcttg gacattgaat ccgacatttt tttcttctcg aatttgtgtc
     5941 gctccgatat ttgaagtcat aatcatgatt gtatttctaa agtccacttt tctaccttta
     6001 gaatctgtta aatgaccatc atccaatact tgcagaagta aattgaaaac gtctggatgt
     6061 gctttctcca cttcatctaa gagaatcact gaatatggtt tttgacgaat tttttctgtc
     6121 aactgtccac cttcatcata ccctacgtat cctggaggag acccgattaa tcggcttgta
     6181 ctgtattttt ccataaattc tgacatatca acacggatca gggcatcttc acttccaaac
     6241 atgacttctg aaagtgcttt ggccaattct gtttttccga caccagttgg tccaaggaac
     6301 atgaatgaac caattggacg atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca
     6361 cgtgaaactg ctttgacagc ctcatcttga ccaacgacac gttcatgcag aagtccttcc
     6421 agttctaaca agcgttcgct ttcttttttc tccaactgct gtagagggac accagtccac
     6481 tccgatacaa ctgtagcaac atcttcttct gttacacgat cagaataacc ttgctcctgt
     6541 tttgcttcaa ttgcagaaac tttttgcagc tttttacgta gtgctttttc ttgtctgcgt
     6601 aattgagcag cattttcaaa gtcttgtttt tggatcgctg cttccttttc ttgtactagt
     6661 tcatcgattt ctagtttgat cacagttgat tttgttaagt gatcggtttg atccaaacgg
     6721 actttcgctg ctgattcatc gatcaaatca attgctttat cgggtagttg acgagaagtg
     6781 atataacgaa cagaaagatt gactgctgct cgtacagctt catctgtgat ctccactcca
     6841 tggtgctctt catatcttgt acgtaatcct tgaagaatca cttctgcttc ttcaggagtt
     6901 ggctcatcta cttgaatgcg agcaaaacgt cgctctaatg cagaatcttt ttcgatatat
     6961 ttttgatatt catctagggt cgttgctccg atcgtttgta attctcctcg agctaacgcc
     7021 ggtttcaaga tattcgaagc atcaattgct ccttctgctc cacctgcacc aattaaggta
     7081 tgcaattcat caatgaataa gattacttga ccatcattat agatttcgtc gatgaccttt
     7141 ttcattcgat cttcaaattc accgcgatat ttcgtaccag caaccaaaga tcccatatca
     7201 agcatcatca ggcgcttttc ttgcatgtct tctggcactt cacctaaaat gattttttga
     7261 gctaaccctt cagcaatggc tgttttgcct acaccaggtt cacccaccag tacagggtta
     7321 ttctttgtcc gtcggctaag aatctggata agccgtttca cttctctcga acgaccaaca
     7381 actgggtcca gcttgttttc ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc
     7441 gtaggtgttc cttgcggtgt ttgccgttta tcttgtacat ttacatttct acgacgattc
     7501 attccttttc cagcagaatc accagctagc cccatttttt gtttcagaac ttgacgggct
     7561 tttgaaaggc tgatacctaa gttgatcatg attcgagtag ccatgatact ttcatctttt
     7621 aataagctca tcaaaatatg ttctgttcca actttttgag aaccagtttt cttcacttcg
     7681 ctgctagcta aagcaaaaac ttgtttcatt cttggtgaat atggaagaaa gactccttta
     7741 gggtaagaac ggataccacc ataaccaatc agatgttcaa tttcttcata aatttccgtt
     7801 tcattcaaat ccatttcacg taatactttt ccagcaattc cattttgctc gaccactaag
     7861 gctagtaaaa catgttctga actaactgat tgatgtttca accgttttgc ttcttcttgt
     7921 gcaatttcta atactgcttg ggctctttca gtaaaaagtt cttccatcta atctcacttc
     7981 ccatcttcat aacttaaacg ttccagaaat gctcggagaa tccgagcacg tgtatgatct
     8041 tcatattcat tgaaattcaa tgtgtttttt gcaattgcac tcaacatcaa gtttccttct
     8101 ttttttgtaa ttatttggtc ttcataaagt ttttgaataa tagcaaaagc atttttctca
     8161 ctgattgtat catcaaataa ctgatttatc tgctcaagta attgtttttt atctgaaatc
     8221 cttacttttg caattcgtat ataaccacca ccaccacgtt tgctttctac tagataacca
     8281 cgttggatgg taaaacgggt attaatcaca tagttgattt gtgaaggaac acagttaaag
     8341 aggtctgcca tttcaatcct tcgaatctcg atcatttcac tattttccag aattttcttt
     8401 atatatgatt caattaaatc cgaggtattc tgatgtgcca tcttattcat tccctttgac
     8461 taatattgac cttaattata acgaatttga taatagaaag aaagaagaac gctttttgtt
     8521 aaataaatcc tttagaatca acaaaaaaaa atacggaata tactattccg tatcgacgcg
     8581 cccaagagga gtcgaacccc taaccttttg atccgtagtc aaacactcta tccagttgag
     8641 ctatgggcgc atattcaatt taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata
     8701 aaaaaaatcg ggaagacagg attcgaacct gcgacccctt ggtcccaaac caagtgctct
     8761 accaagctga gctacttccc gtttaaaaaa taaaaaagac
//
LOCUS       DARBEG010000100         8675 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000100 DARBEG010000000
VERSION     DARBEG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.101"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1101
                     /locus_tag="SCK20_002421"
     CDS             <1..1101
                     /locus_tag="SCK20_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1146447.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="SCK20_002422"
     CDS             complement(1280..1609)
                     /locus_tag="SCK20_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1146448.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="SCK20_002423"
     CDS             complement(1599..1871)
                     /locus_tag="SCK20_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1146449.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="SCK20_002424"
     CDS             complement(2322..3125)
                     /locus_tag="SCK20_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1146450.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="SCK20_002425"
     CDS             complement(3103..4227)
                     /locus_tag="SCK20_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1146451.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="SCK20_002426"
     CDS             complement(4243..5058)
                     /locus_tag="SCK20_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1146452.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="SCK20_002427"
     CDS             complement(5048..5920)
                     /locus_tag="SCK20_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1146453.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="SCK20_002428"
     CDS             complement(6005..7294)
                     /locus_tag="SCK20_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1146454.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="SCK20_002429"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="SCK20_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2691 a         1850 c         1371 g         2763 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacag
//
LOCUS       DARBEG010000101         8497 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000101 DARBEG010000000
VERSION     DARBEG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.102"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(777..2408)
                     /locus_tag="SCK20_002430"
     CDS             complement(777..2408)
                     /locus_tag="SCK20_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG1146455.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(2422..4095)
                     /locus_tag="SCK20_002431"
     CDS             complement(2422..4095)
                     /locus_tag="SCK20_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG1146456.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(4108..4425)
                     /locus_tag="SCK20_002432"
     CDS             complement(4108..4425)
                     /locus_tag="SCK20_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1146457.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="SCK20_002433"
     CDS             complement(4450..5850)
                     /gene="lacG"
                     /locus_tag="SCK20_002433"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1146458.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            6087..6872
                     /locus_tag="SCK20_002434"
     CDS             6087..6872
                     /locus_tag="SCK20_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1146459.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            6875..7567
                     /locus_tag="SCK20_002435"
     CDS             6875..7567
                     /locus_tag="SCK20_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1146460.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7841..8212)
                     /locus_tag="SCK20_002436"
     CDS             complement(7841..8212)
                     /locus_tag="SCK20_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1146461.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         2627 a         1843 c         1448 g         2579 t
ORIGIN      
        1 attataacaa ttgggttaaa aagaaaatag atagtattct acctcatatt tatttgaaag
       61 taatatataa aagacgttca aattcgaacg cctctcgtta accaaatatt ggattcagaa
      121 tctagttttc atatataaac agagtatgat taggtgcaaa aattgcactt ttattgcact
      181 tttattttta tttatagtgt tttactgttc tgtatattgt tttaactccc ttgatagtat
      241 cgcattctgt tttatacact ttacgttgaa aacagagtgg gaataaagag ccaacataat
      301 atggaggggg aatccctata aatcaacatt cttcaatagc taaaatgctt gattttcatt
      361 aaaaataaga tggttcccta ccaaaagaga ctataatata agttaatttg tttcattctt
      421 atcataaaaa gaagttctcg aaaaacgaac tgttttccga gaactttttt ctatccatct
      481 aagtgctcaa atcatattca cgacggaatt ctaaggttac cctttcttaa acaaacttta
      541 tgttatctgc acaacttttt gcaatttttt cattgcttct tacgaaatcg ttgaactctc
      601 tttagcataa tacaaagtgt agatgaagtc tggtgcttta ctcgttttgg taaaaatgac
      661 tggaaatcgt ttctggttat ttttggcaaa ggcgaggtgc aggttcgatt cctgccttct
      721 acgctaacga tgtggctgaa gggaacctcc gatcttgaat cgaaacgatc cgcagtctat
      781 tgtttcacca atgaaacaaa atcaacgttc gtccaagcat ttcctgaagc cttgctataa
      841 accccaattt cacaaacacc tgttgtaatt ggaatgtctt tgattgtaat ttttgtccat
      901 tcacttgttt taacaaccgc agtatttagt tccgctccac cataattttt gatatataac
      961 tgattagtat caaactctcc cccagacatt acataggcac tcaatgtgta agttcctttg
     1021 cttatgccac taacattttg ataagctgat gcctcgtatg cttgatcctg ccaaaaagtt
     1081 agtttgtagt ccccaaaaaa agtgcccgct tccgttttga tcgtaccctt agcggttcct
     1141 tgttgaaacc aggtttgcca attggaagga ctggtcgttg gtccatcgct ttcgaaactt
     1201 gcatttttaa ctagatttat ttgcgcccct gcttgtttaa acgcacgcaa gcttggcaag
     1261 gcgtttccag caaaatcaaa catcgcttgg ttgtcccatt cgtttcccat agtggattga
     1321 acactgagat aattcatacc tgcttgtgtt gcccaactca catttccgtt gtaccaaaga
     1381 ggttcccaat agaagaagcc cgccccgcga ttattcggta ctgcctgaat ccgatcggca
     1441 agatccagca aatactctgc ttgtcctgtc ggtgttggtt catatccccc cgcagctgct
     1501 tcggcttcac caaaagcatt tggcatagta tcggcattcg ctgttgtatg cccataagct
     1561 gtttcaacta cgatcacttc tttaccatac cgttggctga tcgcattcat attggtttga
     1621 agttcattca ttgaaccgtg ccaatacgga taatacgaca ctccaattaa atcaaatgac
     1681 acacctcgac tcgtgatttc atcaaaccac caagtaaatg ctcctgtatc gccaccatca
     1741 gctaagtgga gcattactgg agttgtttta ggttgagtgt ctcgaacagc ttggatacca
     1801 gacttcaaaa aagcagccaa tcgatcaaat tctcccccgc cttctcccca agacttgcca
     1861 taaggccaga gcattccgct attcagttca ttgccgatct gaaccatatc aggataaacg
     1921 tccagctgct tcatttccga cagaacgttc tttgtatagt cgtacaatgc cgtattcatt
     1981 tcttcaaagg tcagaccttg ccatgcttta ggaagattct gtttcccagg atcaacccaa
     2041 aaatcactgt agtgaaaatc cagcagcacc ttaagtccca gattttttgc tcgttttgca
     2101 aggtcaatcg tcgtttccag atcatttgtc cctgctccat aactttctcc attactatta
     2161 taaggatcct gccacaaacg cagtcgaaca tagttgacac cattattttt taaaatctct
     2221 aaggcatctt ttgaaatatc gtcttcataa tattttgcac cagatttttc catatctgac
     2281 aggatcgaaa tatctgctcc gcgaataaat tcgctggcag atgcttcaac agtttttgaa
     2341 acagccccca aagcccctaa cacagctgca aataaaacag ctttacgcaa ccaaattccc
     2401 attttcatta tggctcctcc tttattgaaa ttggttcaga ataaactgaa cagctttttc
     2461 aggatctctc gttaatccga tatattctgc tccttttgtc gcaaccagct tgattcctaa
     2521 gcgatcggtg tcttctttga tatcatcata gtaagaattg acttgcggtg ctaagaccac
     2581 catatcaaaa tcctgcagca tttcgtaatg agaaccataa gcacccgcca ttgcactgat
     2641 cggtgctttc gattcagatg cacctttttt taaagcatta gctaacatcg cacttgtccc
     2701 tgcgccagca caaagtacca acacattgat ttctttattt gcatcgctga taacttgact
     2761 cgctgccaca ggtacttcct cacgtacatc ggcagaagca ccttcctcgg ttgcgatcac
     2821 ctctgctttt tgtgcttctt tctctactaa gacagcatcg tatgctttgc aaaatggtag
     2881 atagatcagg aagtcaacga cgattagaac cgctgctaga acaaaagcta agatacccat
     2941 gcccgttcct agaaccaaac cgatcggtgc tggagttgcc caaggcaaaa cgtacataaa
     3001 gctgttcatg cccaatacat cgacaaaaaa cttgaacagg caaacgttta caacaggtgt
     3061 taacaaaaat ggaataaaaa aataagggtt caaaatcatg ggtgccgcaa atagcaatgg
     3121 ttcgttgaca gcgaaactta ctggaataaa ggaagctttc ccaactgcct tcaactgctt
     3181 agacttagca aagagtagga agataaaagg aacaaccaat gttgccccag ttcctcccat
     3241 agttcctaca aaattcccta aacccaccgt cagtacttta gaagcttgtt caccggaatt
     3301 gaacaattgc agattggttt ccacattggc atagataatt gccgcaattg ccggctccac
     3361 gatcgacggt ccatgaacac cgacaaacca aaacagcgcc atcgctcccc agataatagc
     3421 gattcctaag tacccgtcag ctgctgtaaa tagcggctgc agcaccgtaa tgattgcttc
     3481 tgcaaatgag atttggaaga tttgtcgaat gcctaaatca attaaaacgg ccgcaaatac
     3541 tgaaaaagag aaaggaaaaa tatctcggaa ggtttgcgaa atcgttccag gaacttcttt
     3601 aggcatttta atggtaatat ctttcagcac acaaaactta tataaattaa ctgttaaaaa
     3661 tgcagcgaca aaggaagcca gtagtccctt tgtccccata tattccgttg aaaatccacc
     3721 ttcaatcgga gagacgctta acaacaggaa acttaccaca gaggccagca tcacagaggt
     3781 ggtgttgatt actttgtttt tcggtaagcg acgattcatc gaatcagcca agcagcgagc
     3841 agtggttgct gaaacgagta accctacaac acccatcgaa taattataga ctttccatag
     3901 ccatgtggag acagattcag gccattggta accaaaaatc tcaggaaccg cagcaatcaa
     3961 aatgaaaatg cttgaaaata aaatagctgg catcgctgtt aaaaatccgt cacgaacggc
     4021 tcctaaataa ctattacgtg agattttttc aaaaaatggt ttcccttttt cgatttgttt
     4081 aatgattcca tccatagcgt tcttctccta tccttgattc tgtctgtaca gtacaataaa
     4141 gtcttgaatc agatctctca gcaataaagt ggtcattaaa tgatcctgcc catggataaa
     4201 aatgaatccc atctccatct ctttcccaga tgcttcttcc gctaaaattt ttgtttgcga
     4261 attatgtgca agggttaaat tttcgtcggc ttctttcaac gccgaatcaa catgttcaaa
     4321 gtttccttgt cttacttctt tcaataattt caataatgta ctgcgagcgt cgcctgaata
     4381 tgcaacgatc tcaaaaccaa gcatttgtaa ttcctcttta ttcatctttt cttcctccat
     4441 tatcacttat taaataatcg ttttggtttc actgaccaac ttgtaccagt aagctgattc
     4501 ttttgggtaa cgcttctggg tttcaaaatc aacataaaac aagccatacc ttttgttata
     4561 cccatttgtc catgagaaaa gatccataag cgaccacaaa aaatacccct tcacatttac
     4621 cccggctgtt atcgccttgc ttaaggattc caaatagact ctcaaatagt cgattctcgg
     4681 ctgatccata ataatgccat cttcaaactg atctttatac cccatcccgt tctcagtgat
     4741 atagattttg ttgtaatgtg ggtaatcgct tttaattcgc agcaacaagt catatagacc
     4801 ttctggataa atcagccagt cccaatcagt tcgaggaatt ccttctttat aaatacgttc
     4861 tccgattccc ttcactttat aaacagaggt ccctttttca cccgtaccgt tatgatggat
     4921 tgcattttct ccatcgtaag ctttgacaaa atggcattgg tagtgattga tccctaagta
     4981 gtcattacgc gtggatgctt ttttcaattc aacgaaatct tcttcaggaa aatgatagct
     5041 ggcctgattt gcttcacata tctcatcaag agcagtcaag gtttcagttg aataatagcc
     5101 taaataggta gcatctaata agaaacggat cgacaatgca tcgtctaaaa aggcagcatg
     5161 cttatcttcc ggcgcgtctg ttgccgcata tttcgtctct aatgagtgaa ctacaccaat
     5221 ttcacccgga agttcgtttt ctttgaaata gttcactaca cgggcatgag caaccatcat
     5281 attatgaaga caggcgacga tctttgtaaa atcatatttg attcctggtg ggaaaacacc
     5341 taacaaatat tgattggttg caactggata gatttcatta aaggtactcc acaccttgac
     5401 ttctttgaat tcatgaaaac aaaaaatggc ataggaaaca aatgcttcta ttgtttctcg
     5461 attcaaaaaa tctccatggt caaacaaacg tttaggggta tcaaaatgat gcaacgtcac
     5521 aaacggcgtt acgtttcttt tcttacattc ttcgaacaca cgatgataaa aagcgattcc
     5581 ttctggattt ggtttcccag ctccatcagg aaaaatgcgg ctccaagcaa tggataatct
     5641 tagaccattt acaccaaaac gttcacacaa ttcaatgtcc tttgcatatt gatgataaaa
     5701 atcacttgcc ggatcgggac tgaatcgccc ttgttcctct aaaaagtcat cccaagcaac
     5761 agcgcctttg ccaccttcct tagtggctcc ttcaacctga taggctgctg tggctccgcc
     5821 aaaaataaaa tcttctggta atttaagcat gtacatctct cctcaaatct ataatcagtt
     5881 ttcgctcttt ctaactgatt gcattgtaaa cgcatccaaa acatttgtca acaaaaacaa
     5941 acacaaataa tcataaaaac cagaaaacag caataaaatc tattgtaaca aacataaaaa
     6001 ccaccctaaa tgtttacttt tgttcgaatt agattgactg ttgtccgttt atatgataga
     6061 ttatccttaa attagaggtg aagacaatgt tgaaaaaaga aagattgctt actatcgtag
     6121 aaatggtcaa taaaaaagga atccttaccg ttaatgaaat catcaatcaa ttagatgttt
     6181 ctgatatgac cgttcgcaga gatttagatg aattagaaaa atcgggcaag ctgctgcgtg
     6241 tccatggagg ggctcaaagt ctttcctaca ctttagatca agaactttct cacactgaaa
     6301 aagccactct gcagattcaa gaaaaaaaag aaattgctgc cacagcagcc tccttgattc
     6361 acgagggaga aacgattttc ttaggaccag gtacaacaat cgaactattg gcgacgcatt
     6421 tactgaacaa gaaaattcgg atcattacaa ataattatcc agtttttgat attcttgctc
     6481 aagcagatgg accagaaatt ctactgattg gcggtgattt ccggaaaaat accggcgcat
     6541 ttgtcggacc gatcaccaac gacaatttaa gaagactaaa gtttacaaaa gcatttatca
     6601 gttcaaatgg cgttcacaac gaagcgatct ctacttacag taccgaagaa ggagaagcac
     6661 agcagatcgc tctaaataat tcccgtaaga aatatctact cgttgataat aaaaaattca
     6721 atcgagatga tttttatgtg ttttataatt tgcatgattt cgatctgctc attacggatc
     6781 gccagatctc gcgggaggtt caagagcact atgcacaata tgtcgatctg caaattgctg
     6841 attcaaccga aaataccact ataggagcgt aactatgaaa gggattatat ttgatttcaa
     6901 cggaacaatg tttttggact ctcatctgca tgaagctgct tggctgcata tgatacatga
     6961 tcatacaaaa gatggtttat cagacgagga tattttaaaa aatattcatg gtcggacgaa
     7021 tacggagatt ttgaaacatt tcatttcaga gcatttgacc gcctctgaga tcgccgtatt
     7081 gtcagaagaa aaagaaagct attatcgtat gctttgcttg caaaatgaaa acgagcttca
     7141 gttgacaaaa gggcttgaag cgactcttga ccagctgact gaccaacaga ttccattaac
     7201 gattgccacg gctactgtca aagagaatgt tgctttttat tttgatattt tcgatttagc
     7261 ccgctggttt gactgggaca agattgtcta tgacgacggc tctttccctg ggaaaccgca
     7321 accggatatt tttcttaaag cggcagacaa actgagctta gctccggctg attgtcttgt
     7381 tatcgaagat gcttactcag gattacttgc tgccaaacgt gcggggatcg gaacaattat
     7441 tgcaattgat ccattcggta aaaacaaaac agtatttgaa caggcacagc taaataaaga
     7501 tggaaccatc aaagatttta ctggtttttg gacagagcac ctgtctgcca catccgttaa
     7561 ttcttaaacg attcaactaa ctaccaaaga aaagcaactg aaatgcacac ctccttttat
     7621 aaaaaactgc cagtgaaacg acatgaaaga tacactgcta tatccttagt gaaatatagc
     7681 agtgtatctt tgactttttc attcttttaa ccatatgacg cgttcatctc tatctataat
     7741 taacattaaa aatagtgttt tcacattatt tctcaaacca atcttaaaaa tcatcaggag
     7801 tacttttttg atatactttt attaatgctg ggcgtaatgg ttaaaagatt tccactagtt
     7861 cctctccacc aagctgagtt gaaaaatgct tatctgtaaa caacatagac aaagaggaaa
     7921 taaacgtttc cgtatctttc tctgtcccaa ggatattaat taattttcct actaggtaag
     7981 tatcattaaa cttaagatct gccttcacat caaaagtttt gataaaagct tttatcatgc
     8041 gtacaatttt gtctttatcg ccataattga tttcaccact catatcaata tagtcactaa
     8101 tccctgaaga aataatggca actgcttctt ctttcgcctt atcagataat tcatttaatg
     8161 actgggattg attgatgact tcttgagttt tatctgttac gtaatctctc attatcctat
     8221 cggcatactc cgcaaagtaa tcttgaaaaa attcttctac agggtaacca acttcgtttt
     8281 cccaaccagg agaaatttca ttatcagtac ttattaaatc tattaaaaac caacccgttc
     8341 tctgagtgtt gattctataa tatttttaat ttcctatata aatcaaaaat attatagaat
     8401 caataagcta attaagaaac aaactttaga aactctagaa gaacttctga atttatatag
     8461 aagtgtgtag ctacttacta aatatacaaa agttaaa
//
LOCUS       DARBEG010000102         8494 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000102 DARBEG010000000
VERSION     DARBEG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.103"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..173)
                     /locus_tag="SCK20_002437"
     CDS             complement(<1..173)
                     /locus_tag="SCK20_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146462.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="SCK20_002438"
     CDS             complement(593..1714)
                     /locus_tag="SCK20_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG1146463.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="SCK20_002439"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="SCK20_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG1146464.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="SCK20_002440"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="SCK20_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG1146465.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="SCK20_002441"
     CDS             complement(4304..5215)
                     /locus_tag="SCK20_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1146466.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="SCK20_002442"
     CDS             complement(5232..6239)
                     /locus_tag="SCK20_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1146467.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="SCK20_002443"
     CDS             complement(6236..8365)
                     /locus_tag="SCK20_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1146468.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="SCK20_002444"
     CDS             complement(8370..>8494)
                     /locus_tag="SCK20_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1146469.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DARBEG010000103         8177 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000103 DARBEG010000000
VERSION     DARBEG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.104"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SCK20_002445"
     CDS             476..1339
                     /locus_tag="SCK20_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1146470.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SCK20_002446"
     CDS             complement(1468..3090)
                     /locus_tag="SCK20_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1146471.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SCK20_002447"
     CDS             3339..3860
                     /locus_tag="SCK20_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1146472.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCK20_002448"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCK20_002448"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG1146473.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SCK20_002449"
     CDS             5941..6759
                     /locus_tag="SCK20_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG1146474.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SCK20_002450"
     CDS             complement(6862..7059)
                     /locus_tag="SCK20_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146475.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SCK20_002451"
     CDS             7228..7806
                     /locus_tag="SCK20_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1146476.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SCK20_002452"
     CDS             7901..8038
                     /locus_tag="SCK20_002452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146477.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2445 a         1476 c         1613 g         2643 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttc
//
LOCUS       DARBEG010000104         8048 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000104 DARBEG010000000
VERSION     DARBEG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.105"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..638
                     /locus_tag="SCK20_002453"
     CDS             138..638
                     /locus_tag="SCK20_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146478.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(828..1172)
                     /locus_tag="SCK20_002454"
     CDS             complement(828..1172)
                     /locus_tag="SCK20_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG1146479.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1326..2246)
                     /locus_tag="SCK20_002455"
     CDS             complement(1326..2246)
                     /locus_tag="SCK20_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1146480.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2433..2924
                     /locus_tag="SCK20_002456"
     CDS             2433..2924
                     /locus_tag="SCK20_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG1146481.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2948..5323)
                     /locus_tag="SCK20_002457"
     CDS             complement(2948..5323)
                     /locus_tag="SCK20_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG1146482.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5649..6275
                     /locus_tag="SCK20_002458"
     CDS             5649..6275
                     /locus_tag="SCK20_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1146483.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6298..7002
                     /locus_tag="SCK20_002459"
     CDS             6298..7002
                     /locus_tag="SCK20_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1146484.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7194..8027
                     /locus_tag="SCK20_002460"
     CDS             7194..8027
                     /locus_tag="SCK20_002460"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG1146485.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2474 a         1527 c         1454 g         2593 t
ORIGIN      
        1 tattgtaatt catatttgta ttgttcgatg attcttaata cgatatgaca caataaataa
       61 aaataggcat ggctatttac aacaatagtg ctatagtaga aaacaaatga ataaggttac
      121 ttaaagaggt gaatacgatg aaactgatca aactatggga agcagattta aagaaagctt
      181 atcagctaca aactagcttt ggaccaaatg agagtggatt tatcaacaat gcttatggct
      241 atacatttgc tgaattttta gattatgtaa aaaaatgcga aaaaacgtct ttaggaatcg
      301 atcttcctga gagttacgtt cccgcaaccg tctttatttt agaaagtgat gaacatgagt
      361 atgtaggaat ttttaatttg cgccataaac tgaacgaaac attaagaaac ggcgctggcc
      421 atattggtta cggcatctcc cctaattatc gtaaaaaagg atatgccaca caaggattag
      481 cattagtact acaagaggca aaaaaaatcg gcattgatga ggcacttttg tctgtaaata
      541 aaaaaaatct tggcagtctt aaagcacaac aaaaaaatgg ggctgcgatt tatcgtgaag
      601 atgagaaaga atactatacg aaaatcaaac tgatgtaaaa aagaaggatt ccaaaaggaa
      661 agggatttga atgtccttta tcaatagaac acatttattt aattaactta taatatagca
      721 gacaaaattt tataaacaaa gaaacctttt atctcttttc ttctagaaag gataaaaggc
      781 ttttttgttt ttcctatttg cttctcatag ctagtcagcc aagatttcta tcgtatcccc
      841 aacgcaatcc gtgcataacg gctcatctta tctgtcgtcc atgctggata ccagactagt
      901 ttgacttctg ctttcgttac ttctggcact tcttttaatg cgtcatggat agattctgtt
      961 aaaatatctg ctaatggaca gcccatagtt gttagagtca tcttaatgac tgtttctcca
     1021 ttttcaggat tgaattctat atcataaatc aagcctaaat tgacgatgtc gattcctaat
     1081 tcaggatcaa tcaccatttc caaggcagca agaatacgtt ctttgattag ttctatttct
     1141 tgtccagacc gttcttggtt tgcttcgctc atcgtacgac actccttaac acgattttta
     1201 tttatcatac tcttttttta gctgcttgta aatgagaatt tagaagtaag attctcgttt
     1261 aaatttataa aaatgttctt attagatgta atagattaaa tttattctaa aataaacctg
     1321 attcctcatt ttttttgtga acgatttttt atttctggca atacgccagt tgttcgatac
     1381 attttttgtt cgttttcatt tgcaaaccgg ccaaatactg aatgagttgg taaaatattg
     1441 ataaatgcat cgtcgtcgat ttcatgaacg atctgatcaa gatcatacat ttcgtagcgg
     1501 gtgatgacca tcatgatcat gttcccttgt ttttttgaat agccaccgac agacggcagt
     1561 aatgtcattc cacgtaccat ccgttttgct agttcttctg ttacctcttc tggcttgacg
     1621 gtaacgatca ttgctgttac tttttgatga ctcgtgtgga tggcatcaat tacttgagtc
     1681 attgcataaa tggaaataat cgtatacaag gcactttccc agttaaagaa gaagcctgcg
     1741 atgactacaa tgataccatt tagaatcagc atgtagtttc cgactgtttt tccagttgtt
     1801 tgtgaaagta caagagagat gatatccatc ccaccagttg taaagcccat ttttaaagat
     1861 aatcctacgc caagacctaa taaaacaccg cctactaggg cattcatcaa aatattatca
     1921 gtgatctgtc cgactggaag cagcatcgtc atcaccgata cacatacaac attcacaaaa
     1981 ctaaggatcg ttgctcgttt tcctactttg agaaacccta atacaaacac agggatattc
     2041 aaaagaaaaa taaatgttcc tgtgtctatc ttgatatgaa accattcata taaaagcgag
     2101 gaaatgattt gagcgatccc attcatccct gcagaaaaga cattggctgg aatcaaaaaa
     2161 taatttaaag cgaccgcagc ggtcaatccg gttatcaaaa taaccactat tttctttaat
     2221 ctttcatttt tatgatacgc agtcataatt ttactcacaa gcacctctcc cctactttgg
     2281 tattccaaat gtaagaataa cagtttatct attaaaataa aatacttcga actcaaatga
     2341 gaagaatttt agcaaacagg taagaatctg ccctaatcct atgataagat agatgtatgg
     2401 taaatattta tatgtatatc aggagggttc ctatggcaaa agatgctagc ttcgacattg
     2461 tatcagaagt caatacagaa gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt
     2521 ccaatcgctt tgacttcaag gattctacag tagaaatcaa attagagaac agcaatctag
     2581 ttgtattgag cgatgatgat ttcaaaatcg aacagatcaa agatgtatta tttggtaagc
     2641 tgatcaaaag aaatgtgcca atcaaaaata tccatttttc cgaatcagaa cacgctctag
     2701 gtggcaaagc tcgacaaact gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga
     2761 aaatcactac agctatcaaa aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc
     2821 agattagagt gacaggtaag aaccgtgatg atctacaagc tgtgattgct ttattacgta
     2881 aacttgactt accaatcgag ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg
     2941 acgtctgtca ttcctcgttt ttttgattta ctgcttcttt tgataaccaa aaacgctgta
     3001 ttccttcttc caattcccca acacttcgta cttctcttgc tgtttgccaa tgttgtggga
     3061 ataaacgtcg ataagaaaaa ctagaaaatc gttgatctgc caatacaacg attccttgat
     3121 ccgccatgcc acgaatcact cgaccaccag cttgcaagac tttgttcatt ccaggaagct
     3181 gataagcgta agcaaatcca aggtgttcct tctcatcata atatgatttg atcagttcct
     3241 gctcatgatt catctgcggc aaacctacac ctacgatcat actgccgatc aatcggtccc
     3301 ctctcaaatc gattccttct gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt
     3361 cagcgggatt tgtttgaaat tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg
     3421 tttcttggat tagcacattg ctgtcgggat attgctgtct gaatgcttcc accgcaagat
     3481 ctaaatattg ataagaagga aagaaaacga aataattccc gacctttcca tcagcaaaag
     3541 catgaatcgt ctctactaat tttgccagac tgtgttccct ttttcgataa gtcgtctcta
     3601 ggtaatttgc gatgaaaact tgctggtttc cttctgggaa aggactcggc agtttgtaag
     3661 ccagactttt ttgtcccccc agtgtttcct gataataaga gagtggtgaa aaactagcag
     3721 aaaaaagaat actgcttttt cctttatcca acgactgctc aagaaacaag cttggatcaa
     3781 tacaaaactc ttttactatc agatcgcgat acgttttttc gactgttgtt tcgtagtgat
     3841 catcataaaa ttcactgacc ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt
     3901 gttcttgtga tgggtgctca ggaaattctg ccagccattc tttaattttt tcgcttaatt
     3961 gataacctgc ctttactaaa gattcagccg gtgctttttg atgatgataa tcccaatgat
     4021 cttcttttgc tatttctcgg atcgaatcaa attcttttaa tagtttgttg aaacggcgat
     4081 gaagtttttt gaattccttt ggtatagctt ccttacttcg tttcgttttt tcataagata
     4141 gttctgccga atacatctct cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa
     4201 acagatagtc ctcgtttttt tcatcttcaa aaaaacgtct taaataaaca gttggatcaa
     4261 ataaataatt gtaatcccca atgattacgt cacaccagat actgacatct aatgatagtt
     4321 cgaaaggaca aagtgtatgc tttcttgcat aagtttcgat gacttcacga gtgatctgat
     4381 tctcatgatt caaaagatcc cataaacctt cattaatccg attgtaataa ccattggcat
     4441 atgggcactg atctggatta caggtcgtct catctaaaaa gcagatcttg tctttcgccg
     4501 tcaaagtgac actttttact tcagctcctg tatcttttaa tgctgtcata gcatcttcag
     4561 ctacttgtct agttattgtt ttagccgtta gataaaagat cttttctccc tcttcttccc
     4621 ctacagcttt caaagcgggg aataacgtag agattgtttt tcctgttccg gtaggtgctt
     4681 ctacaaacaa tttctgtttc gtacggatcg ttttataagc agctaccgct agttcacgct
     4741 gacctttcct atactcatca tgggggaatt tcaaagccat caaagaggca ttgcgtacag
     4801 tgcgccagtt ttcttgaaaa acaagccatt ctttataatc ttcagtcagc tttttaaaaa
     4861 aagtttctaa ctgctctctt gtctgatgtt caacctttct cgtgatcact tcttctgccg
     4921 tctggaagta agtcaattgc agatttattt cttgtaaatc atgttcttga ctatagatat
     4981 aggcatacac ccttgcttga tgaaagaaaa gttccacttg ttctggttct aaatcttcaa
     5041 atcgtggttc ggaggttttg atttcatcta tataataaat accgtctctt ttgaaaatac
     5101 cgtctgcacg gccttcaacg atcaggtaat attcatctaa ttgaactgtt gtttttaaaa
     5161 agacttcttt ttcatagttt tctcccgcat ctttttggag cttgcggtgg attctcgctc
     5221 cttctaatgc agtatggtta ctttttttac gattgtcgat acttccacgt cgcaaaataa
     5281 attctactaa gtgccgcaca gatatttttg actgtttgat catttccacc acctctttct
     5341 ctctaagatt acctgaaaaa acctagttta gcaaaaagag ctctctttcc aaacataaaa
     5401 aaacaccctg ctgattaggc agggatatag gatatagaat ttggattttt ggatgaatct
     5461 aatgtaacaa ggacatatta aaatgctatg aaaacttact ttccttctct ttagaatatc
     5521 caaatacttt tcatatagtc tgataattca gataagtaaa gagaaataag tacctgtttt
     5581 tgtttcctag caatgaatgc gttatactaa tggatgaaag aagttttggc tatcaaggag
     5641 gaatgcacat gcatatcgaa agattaaaaa caggaatgat tgaagaaaac tgttatttgg
     5701 tctacaacga agaagcactt ttgatcattg accctggaga agatgcggaa cgaatcaaac
     5761 aagaaatcaa aaaaacaaac caacagccgg tagcgatttt gcttacccat actcactacg
     5821 atcatatcgg tgcagtggaa cctttacgcg cgtattatca aatcccagtt tatgtcagcc
     5881 cactagagca aaaatggcta ggtgatccga ttttgaatct ttctggactc ggtagacatg
     5941 atgatattgc tgatgttatt gtctcgcctg cagaatatga atttgaaatg aaaacttata
     6001 ctttaggaaa catgtctttt cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca
     6061 gctttatttt tgatgatttt gtcgtttctg gcgacgccct gtttagagga agtatcggta
     6121 gaaccgatct gtatacaggg aatttagaac aattacttca tagtattcgg acccagctgt
     6181 ttgtattacc tgatgaattt gcagtctatc caggacatgg cgatgcaacg acgattgaac
     6241 aggaaaaaag gacaaatcct tttttcaatc gttaaaacga cttaggagga gaataaaatg
     6301 acagaaacta cactttatat cgtacgacac ggaaaaacga tgttcaatac aattgaacga
     6361 gtacaaggct ggtgtgatac acccctcaca aagcaaggac aagaaggcat tcattattta
     6421 ggtcgcgggt taaaaaatgt cgacttttca tttgcttatt ctagtgacag tggtcgtgca
     6481 atcgaaactg cccgtattat tctaggagaa cattctaagg gaaaagaaat tccttatttt
     6541 attgaaaaaa ggattagaga atggtgtttc gggtctcttg aaggtggata tgacatggaa
     6601 atgtggggtg tgatccctcg tgtattgaat tttaaaacat atgatgaaat gtttaccaca
     6661 gaagtttctt ttgaacaaat tgctaatgct gtatacaact tagataccgc tgggtgggcg
     6721 gaaacttatg aaacattgcg agaccgtgta tggacaggat ttgaagatat cgcccatcac
     6781 tgcgaaaaaa atggtggtgg aaatgtttta gtcgtatctc atggtttgac gatcgccttt
     6841 ttgcttaatc tgatcgatcc taaacagcct gttcgtgctg ggcttttaaa tggcagcgta
     6901 acaaaggtca cttatgcaaa cggtcgattt tctatccacg gcatcaatga tacgtcttat
     6961 atcgataacg ggaaaacaca tagtcgcgac tcgtttctat aatggggata ttttaatttt
     7021 taccagagaa tcataaaagg caaagtctga acgagggctt tgtcttcttt tttctcttcg
     7081 tgtacttatc tgctttattt ctcaagtttt tcttaatatt aaaaaacaga ttgggtttct
     7141 caaaaaataa cggtatacta taagatgtat cttaattggg aaggaaggaa tttatgtttt
     7201 ttgcaaatat tatcaaagca gtgattctcg gtattattga aggaataacg gaatggctgc
     7261 ccatcagtag tactggtcat ttgatcctag cagatgaatt catcaaactg gatgccagtt
     7321 cacaatttat gtcgatgttc aatgtcgtta tccaattagg ggcgatcttg gctgtcgtcg
     7381 ttctttattt tcacaagtta aacccatttt ctccaagtaa atcaacggta gaaaaaaaag
     7441 atacgtggac actttggttt aaagtgatcg ttgcttgcat accagccatc atcattggtt
     7501 tgcctttgga tgattggtta gaagcaaatt tccacaagtt cttaccagtt gccattgttt
     7561 tgatcgttta cggtattgcc tttattattg tcgaaaaacg caacaaaaac aaaacacctc
     7621 gttggacttc tctgaatgac tttacgtacc aagcagctct tcttgttggg ttattccaag
     7681 tcttggcact tgttcccgga acatcccgtt ctggcgcaac gatcctagga tctatcttga
     7741 ttggtgcttc gcgttttgtc gctacagagt tttcattttt cttagggatt cctgtcatgt
     7801 ttggcgcaag cggcttgaag atcgtcaaat atttagctga agggaattca ttcaaaatgg
     7861 aagaaactgt tgttcttctt gtaggaacat tcgtatcttt tgtggtttcc gtcttagcta
     7921 tcaaattctt actaagctat ttgaagaaaa acgactttac cgcttttggc tggtatcgta
     7981 ttatccttgg tatcattttg attggttatt ggtttattgc aatgtagaaa aaaccagaac
     8041 acttctct
//
LOCUS       DARBEG010000105         7829 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000105 DARBEG010000000
VERSION     DARBEG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.107"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..995)
                     /locus_tag="SCK20_002461"
     CDS             complement(21..995)
                     /locus_tag="SCK20_002461"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1146486.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="SCK20_002462"
     CDS             complement(1075..1950)
                     /locus_tag="SCK20_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG1146487.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="SCK20_002463"
     CDS             2080..3987
                     /locus_tag="SCK20_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG1146488.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="SCK20_002464"
     CDS             complement(4528..7806)
                     /locus_tag="SCK20_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG1146489.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2204 a         1658 c         1458 g         2509 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatt
//
LOCUS       DARBEG010000106         7822 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000106 DARBEG010000000
VERSION     DARBEG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.108"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(101..1249)
                     /gene="nagA"
                     /locus_tag="SCK20_002465"
     CDS             complement(101..1249)
                     /gene="nagA"
                     /locus_tag="SCK20_002465"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG1146490.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1325..2161)
                     /locus_tag="SCK20_002466"
     CDS             complement(1325..2161)
                     /locus_tag="SCK20_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1146491.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2345..3562)
                     /locus_tag="SCK20_002467"
     CDS             complement(2345..3562)
                     /locus_tag="SCK20_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1146492.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3711..3890)
                     /locus_tag="SCK20_002468"
     CDS             complement(3711..3890)
                     /locus_tag="SCK20_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146493.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3945..4028
                     /locus_tag="SCK20_002469"
     tRNA            3945..4028
                     /locus_tag="SCK20_002469"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3979..3981,aa:Leu,seq:gag)
     gene            complement(4268..5638)
                     /locus_tag="SCK20_002470"
     CDS             complement(4268..5638)
                     /locus_tag="SCK20_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146494.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6352..6642)
                     /locus_tag="SCK20_002471"
     CDS             complement(6352..6642)
                     /locus_tag="SCK20_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146495.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6850..7542)
                     /locus_tag="SCK20_002472"
     CDS             complement(6850..7542)
                     /locus_tag="SCK20_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1146496.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2397 a         1542 c         1194 g         2689 t
ORIGIN      
        1 agaaactttt gaaacttatg taaagtgatt tttactagaa cacaaaaaaa ccttaaaacc
       61 aaacttaaga agtttagttt taaggttaac ttaaattata ttatgcgtgg aaacgttcca
      121 cgccatctag ataagttgct tccaatgtca tatcagggtt caatacgata aagtctgcat
      181 cgcgtccttg agcgattttt ccacatgtat catcgatttt acagctgatt gctggaatca
      241 gactagccat catgactgct tgttctggtg ttgctaatcc ccagtctaca acatttttaa
      301 ttgcttcttt caatttcaat atacttccag ctaagttgcc agattctaga cgtgctgttc
      361 cttcttttac tactactgga aattcgccta aattgtaatt tccatcaggc atcccaccgg
      421 ccatcataca gtctgtaatt aatgcgacat gatcatgtcc tgctttttcc attaggattc
      481 ccgcagcatt tggatgtaca tgatggccat cacaaatcaa ttcagaaaag acatgttcta
      541 aagataataa cgcacccacc atacctggtt cgcgatgatt caatccacgc ataccattga
      601 aagcatggac aaatacactt gcgcctgctt caacagcttt ttgcgcttct tctagtgtag
      661 cattactgtg tcctagagaa acaactactc cttcttcagt cacttgtttg acaaactctt
      721 ctacaccttg acgttccggt gctaaagcaa tttttttgat taaaccacct gaagcttctt
      781 gccattcatg gaaagtatcc aaatcaggat ccccgaaata acttgggttt tgtgcccctt
      841 tgtgttcttc tgtgaagaaa ggaccttcaa aatagattcc tttgatttta gctcctggtg
      901 cttcctgata cattttgccg attgtctcag ctacatcttt taaacgttct ttgcttgacg
      961 tcaatgttgt tggtaaaaat gaagtcactc cacaagataa cagaccttca gacatcactt
     1021 tgatgccttc tgcatcatta tccattacat catgattcat atagccgtga atatgtgtat
     1081 ctaccaaacc aggagcaatc catttgccgc tttggtcaat gatgttgact tctccctcag
     1141 gtagttcttt ggtgtattca ccaaaaagtc catcggtaat ttcaagatag cctgcatgtt
     1201 tgacacctga cgctaaaaag aatttgtcag catagataaa agttctcatt ctattctctc
     1261 cctttgtttt tctacctcta aagtactctt atttagagaa gaagtcaaga atggtctaca
     1321 ccatttacaa attttttgaa atttgtttca cagcttgtgc taagccttca tagaatgctg
     1381 ctctttctgg ttcttcttta cgcttgtata ttaagctttt aagcacaaat aaatcattga
     1441 ggtaatagta gctattcttc tcttgagccc aatagattcc ttgattcacc tgttctaacg
     1501 cttcgtcgat ttcattttga tggaataaga aatttccata attataaaaa atttgcgaaa
     1561 attcactttt tgatcgttca tcagctgatt gatgcagcag ttggatgctt cgactcaaat
     1621 agtagcgtgc ttcagccgtt tttcctgcca caccatatat ttttccgata caactaatca
     1681 gttgtatttc tgtgtcagaa acatggattc tttctttttg gctagagtag cttaatgctt
     1741 ctttcaaata ataaagggac tgagagatat tttgacgcaa cgtgaattcg caaacaccga
     1801 gataataata atagcgttga aagtcagttg cttgatacag attttcagaa atatcagaag
     1861 attttagtac ttgttctaac ttccgataat cacgtttttg gaaacaacta tccaacagtt
     1921 caaataattt atcgtttgtc cgagtagttt ttcgagagag attcatcaca tgatcaatcg
     1981 ttacccctag ccgttgacaa atctgcatca tcaccaatac attcggcagt tcctcattat
     2041 tttcgatccg acttaataca ctttgtgaac aaatatcatg cgaaagcatt ttttgcgtca
     2101 ttttttgttc ttttcgaatc tctttaatga tcgttccaaa ggactcgatt aacgtttcca
     2161 taagtatcct ccccaaaata tgcgtattcg cattctattc gtattttact ttaggaagtc
     2221 gcaaacttca agaaaagact tgcaactaaa taatatactt tagttattta agaaaactcc
     2281 actagaaaga gagcctcagt gtgtttgtct gtggccctcc tactgaaaaa tttggttttt
     2341 aaaatcattt ttggcgatag gttgctagga attctctcat tttcttagct gtttgtttca
     2401 aatcatccat tttaggaaga taaacgattc gaaaatgatc tggctggttc caattaaatc
     2461 caccaccatg tacaagtaga atatgatgtt catgtaaaaa gtccaaaacg aatttttcat
     2521 catccaaaat attgaatcgc tttgtatcaa tttttgggaa tatataaaaa gcagcttttg
     2581 gttttacggc tgaaagacca ggaatatcat tgattgcatt atagatatat tctctttgtt
     2641 catagatcct tccgcctggc aggagcagct catcgacact ttgataaccg cctagcgcgg
     2701 tttggataat ttgctgagac aatacattgg aacacaaacg catggaggac aacatattca
     2761 gcccttctat atagcctttg acgtgtgact tatctccact taggaccatc cagcctactc
     2821 gaaatcctgc tacacgatga gatttggata gaccgttcag cgtaacaacg aacaaatcag
     2881 gcgctaatgt agcaatcgga atatgttcta gaccatccat cactaagcga tcatagattt
     2941 cgtcggaata aataatcagg ttattttgtc gagctacttc aacgatttct tctaataatt
     3001 cttttggata aagtgcccca gtaggattat taggattaat gataacaata gcttttgtcc
     3061 gactcgttat ttttgatttg atatcatcaa tatcaggata ccattctgcc tcttcatcgc
     3121 aaatataatg aacaggtgtg cctccagcta atgcaataga agccgtccac aacggatagt
     3181 ccggcattgg aaccagtact tcatctccat tgtccagcaa cccctgcata cacatcgtga
     3241 tcaattcgct cactccgttg ccggtgtaga tatcattgat cgtcacattc ggaaaatgtt
     3301 tcagttggca atattgctca attgcttttc tagcagaaaa gatccccttg gaatctgagt
     3361 agccttctga gtcacgggca ttcatgatca aatcacgaat gacttcgttt ggtgcttcaa
     3421 atccaaaagg tgcgggattt cccgtgttca attttaaaat actaatgccc tcttcttgca
     3481 tccgctccgc ttcttctaga acgggaccgc gaacatcgta actgacacct tctaatttgt
     3541 tggatttttc aaattctctc atcacttagc caatccctca tttcaaaaag ttagaacttt
     3601 ccaaattttt tgttatttac tatttcaata tcatactcca tcctagaaaa ttttgccact
     3661 tatttaggaa aaacgtaagt attaaaaaaa cacaaacaag cgttgagaac ttaattgtca
     3721 tgatagtcat tccatagatt cgctgcccaa tccattgcaa ttccacaaat gattcctagc
     3781 ataaaaccaa gcaaaaaacg tccgaatgca agtccaatca atacacctaa gatcattccc
     3841 agtcctgaaa attctccgtc atatttctcc atcttaataa gtcctcccat ttttgagctt
     3901 gacaactatg taaaacctcg ctatcattag tgtgttatta ttacgcagat gtgttggaat
     3961 tggcagacaa gcatgattga gggtcatgtg agcgttagct cgtgtgggtt cgagtcccat
     4021 catctgcact acaactcgtg aagttaattt tgcttcacga gtttttttgt ttgaacgttt
     4081 ctttatgcca ttatatacat atcactgtat cctttacaaa aagaacgtac aatcatgtaa
     4141 acagcctgag gtgagtatat gaaggtcgga aacaagctac tcgtagaaac ttgcaagtgt
     4201 taaaacaatt aatcgaaaaa cgagtaaaat ccctcaccta atactaaagc gagggatttt
     4261 ttgtatatta tctgtttatc accgttatta taaaggtaca aataccaagc acggttaatc
     4321 caaattgcat aaattcattt ttttgtacac tcatgctttt cattagctct ttaactgcca
     4381 ttagtactaa gatggcgata acaacataaa gtatatagta cgtccgcatt ccggaagccc
     4441 atactgtggg agaaaatccc ataattaacc ttgaacaaaa tcccattatc atcaaaataa
     4501 ctaaaaaaat agaagttttc ttattgtcaa aacttaaata aattccaaca attaaacata
     4561 gtaataatgc caagactact aaagtagcta tccaagtacc tggataaaaa agtgacagtt
     4621 tcgttcctaa ctctgtaaag agattattca ggttactact actccaaatc attgcccttt
     4681 tatttccatt gacgtaagta aagctttgac ccattgtatt accaaaataa ataatcaggt
     4741 ttaagaaaaa cggtaatgca gtcaatatac gcacataata gttttgacat ttcctatatg
     4801 ttagaaggaa aattaagaaa aatagcaata aaaataagat attcatgtct aaaaaaaacg
     4861 gctttccaaa tgatgaaata cctaaatcta acttattaag aaagttgaaa tttttatatt
     4921 caggaaacca tttattaatt tcatgtgcag cacgtaatgc attccctgga gttgtcaaag
     4981 agaaaattaa ttctgctaag cttattattg aaaatactga taacttataa ttatatcgtc
     5041 cattgacaat caaatataaa ctaactatac ttgttagaac aaaaaaacag gcgttgactt
     5101 gttcttgatt agttaaaaat atcaataaag gaatacttac ccaatatatt tttctattta
     5161 tttcttctcc tcttaagagt tgaaaaaacg gataaaaagt tagtaaacta aatgctactg
     5221 gccactgata gttcaaggtg gttgctctcc aaccggtttc ccccattact gtcaatggta
     5281 ttaaacagta tatagagcaa accaataata aatttttaac atttattttc tcaaaaacat
     5341 acttgcaaag taatactaca attcctaaca taatcgtaga atttaataat ttccatagca
     5401 taaagtgttt agaaaagaac attaagaata gttctataat aattcttgaa ctccatgtct
     5461 tataatgttc tatcgaagca cccaaatagc caagttcatc taatctactt gcataaacta
     5521 cgtcatccgc tctcatagtc ggcatataca tattaaagag taaaaagata ccccaaatcc
     5581 ctattagtaa caagatcagt tctttgttat tcgttatttt tctattatgt ttgttcattt
     5641 ttatactcct tcttatattt cctaatagag tataacatag ctttaagcat tacttaaaga
     5701 taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa
     5761 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
     5821 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
     5881 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
     5941 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
     6001 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
     6061 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
     6121 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
     6181 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
     6241 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
     6301 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
     6361 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
     6421 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
     6481 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
     6541 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
     6601 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
     6661 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
     6721 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     6781 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
     6841 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
     6901 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
     6961 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgagtttcta catttgtttt
     7021 ttcttgtgtc agtgttgcga tttgttgatt caaaccattt acagtatttt gtaattctga
     7081 aagttttgct tgtgcttctt gtagtcttgc tgtccaatct gcattcagat gattgatttc
     7141 atcatttttt tggttgatct catctagttt gacattgatt tcattttgtt tgctttgtag
     7201 ttgtgcatcc ttgctgacta attgactttc taagttttgt ttttctgctg ccgattgatt
     7261 tttttggttt tctaattcat ttacttttgt ttggtattct tggacatctg cctttgaatc
     7321 ttgcaatgtc tgttcttttt gtgctagtcg agcggtaaga tctgtaagcg cttgctcttt
     7381 atctgtcaat gtatgatcaa gctgtgtcag attctcattg attgtatcaa tatctgtctc
     7441 tccaccccac aaaataattt catttgcgta ccgcgtgcct gtgtagccaa ttagacatag
     7501 aaatgcaacg acaatcccac cagtaataaa tttctttttc atttgcatag ctccttaatc
     7561 caatactttt acatgctaaa agtaagtgat ctcaaacaca atgtcaagcg tttttaaatt
     7621 acatgtgtaa ttttaatata aatactatca aatactcatt taatcctcct tttttatcta
     7681 tttaaaaaat cttttttata gagaaaaatg caggttaata ctttcttaaa taaatattta
     7741 tggtgaacaa aaacaaaaaa gcaagtttta ttaaacgtaa cccttttcat ttcatagatt
     7801 actgtattta ttacaaaaaa cg
//
LOCUS       DARBEG010000107         7592 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000107 DARBEG010000000
VERSION     DARBEG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.109"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..787)
                     /locus_tag="SCK20_002473"
     CDS             complement(59..787)
                     /locus_tag="SCK20_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1146497.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(800..2080)
                     /locus_tag="SCK20_002474"
     CDS             complement(800..2080)
                     /locus_tag="SCK20_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1146498.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2221..2823)
                     /locus_tag="SCK20_002475"
     CDS             complement(2221..2823)
                     /locus_tag="SCK20_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1146499.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3068..3274
                     /locus_tag="SCK20_002476"
     CDS             3068..3274
                     /locus_tag="SCK20_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146500.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3586..4305
                     /locus_tag="SCK20_002477"
     CDS             3586..4305
                     /locus_tag="SCK20_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1146501.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4386..5069)
                     /locus_tag="SCK20_002478"
     CDS             complement(4386..5069)
                     /locus_tag="SCK20_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG1146502.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5287..7473
                     /locus_tag="SCK20_002479"
     CDS             5287..7473
                     /locus_tag="SCK20_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1146503.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2720 a         1281 c         1427 g         2164 t
ORIGIN      
        1 agaaccgaag ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt
       61 atgaaatcaa tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg
      121 tgacttctct ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt
      181 tatgacaacg gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt
      241 gtaattgggc atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa
      301 aatattggat gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga
      361 tatctttttg gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga
      421 cattttcttt taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc
      481 tttttaagat tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag
      541 gatctaattc acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt
      601 cgatatctaa aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt
      661 cttccggatc agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat
      721 ttttgatcac cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg
      781 gaatcatatc attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt
      841 tttcaattga tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa
      901 acaagccaag gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact
      961 ggtttaacgg taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct
     1021 tcttgagtat ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat
     1081 tgtccaagat aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga
     1141 ttggctcagg aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta
     1201 atgacagtaa actacggatc tcaggaactt tcatattatc caacatactt agattggccg
     1261 gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc
     1321 cttctaagtc gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat
     1381 gtttgaattc tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca
     1441 tcctgacttg ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat
     1501 aaaccaagat gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta
     1561 agacatcagg tgtttcacca gaaaaacggg tataaaaaat aatgatatag tatgtaaata
     1621 atatcaatgt cccgatcgta ccgatcatac ggcggtccac agtgtgaaac caagtctcta
     1681 acgatttttt taaacaaaaa gctaataaag cagataaaaa agcggataag acaatgtgaa
     1741 aatactgcaa tgaatctcct ggttcaataa ccgaagattt caaaacaaaa aaatcgtcga
     1801 tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc aaaaggaagc gcgtaaacca
     1861 attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat cagcattcct atagcaatta
     1921 gagcagtaaa aatccctaaa atcgaataga cccatccaat caaaatacaa gaaatcagaa
     1981 tgactcccgc aaacagatat ttgtatcgga tgtgatggtc aatcaataag atacaaattg
     2041 tgtaaatcaa atattgcaac gctatggacc ataaaaacat aaagtacagc tcccattgat
     2101 ttttaccaaa ttatagcaca attagcgtat tattttttta atttttcagg gattttgaat
     2161 tcagaactca taccaaagca gcagccagat acaaaaaagt cagataagtt agttaattgt
     2221 ttaatgattg tttttttcat gaaagattcc tccaaaattc atcaagtagt ttgtaatagg
     2281 aagcaacgta gctatttcta aaacaatccc aagtagcaaa taggtagaaa agtaagaagc
     2341 tgagaaaaat aaagtaatga gagaaaaaat tagaaacatg ctgatcgcct tctttaataa
     2401 tgggggacgt ttctcgggtt caatgggatt gattgctgta cctcttgatc catagcgaag
     2461 cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt gggaaggaga tagtcaattg
     2521 cagatgtttc atcaccaagg gaacgcccca taaataaagt agtgtccaca gcagacaaac
     2581 gaattcagag tcggaatgca atccataagc atatctgcgg atgcagatat aagaaagaaa
     2641 ggtgattcct gtttccgccc atgagcctgt caccagagaa aagactagaa cgaccgctag
     2701 ttttccaaga ttattcaata aaacttctaa ggcaaatttt acttggacat acaccatatc
     2761 ttcttccgat ttgtctccgc tgaataaggc ttctaataat ttgttcacaa ctttatcttt
     2821 catctcatac gtacctccac cacaaatttt atgctaaaaa aaataaaaaa cgtcttttgt
     2881 ttcataaaaa gtggttttga catcaaaacc gtcatgatct cctaaaaaaa tcttcgtttt
     2941 ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa aagtactttt tatgacaagt
     3001 ttgtgctttt tcgagtgatt tatgttaaaa aattactgac cttataaaag gtgatggagg
     3061 gattcttatg gaaaatgaac aattgtttga agtcggcgaa acatatgcat ttaaatctga
     3121 tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc tatgaaaata gtgtgatgat
     3181 acgtgtcatg aaatgcgcag tagaagacaa tgataccgct cataatctgc actggcagtt
     3241 agttgtacgc aaatcaagta caatagaaga ataaaaacag ataaaatgtt gagagcataa
     3301 agaggttgtg agtctcccgt ttagctctga gttacaagga ataatgttaa aaaacagctt
     3361 ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg caggagaata ctgtttattt
     3421 gaaaaaaggg ctgcgacaag acttttgtca cagcctttat tttcatgaat aggagttact
     3481 tttggctact tttagagaag tagcttttaa ggagcagata aagtcaaggc ttttatttct
     3541 ataaatgaag tagtaaaatg aagaacagat tttattacag gaaggatgcc gataattgtg
     3601 atcattgttg caggaacgat cggtgcaggg aaaagcacac taactgaaat gcttgcccaa
     3661 gatttagaaa caaaaccttt ttatgagaac gtggaagata atgaagtatt gccgctattt
     3721 tattctaatc cagaaaagta tactttttta ttacagattt tctttttgaa caaacgcttt
     3781 ttggcaatca aagatgcttt cagccatgat gacaacgtac tagaccgctc catctacgaa
     3841 gatagtatgc tgtttcattt gaatgcagat ttagggcgtg tttctgaagt agaagtaaag
     3901 cagtatgaag gtcttttaga aacgatgctg aaggagttag aagaaatttc gccacaaaag
     3961 aaaccagatc tactggttta tattcgtgta tcctttgaaa ctatgttagc gagaatcaaa
     4021 aaaagaggcc gggaatatga acagctagaa caagatcctg aactctattc ctactataaa
     4081 gaactcaatc gacgatacga agagtggtat gaacagtttg atatatgtcc caaaatagtg
     4141 atcgatgggg acaaatatga tttcgtagcc gatccggcat gtggagaaca aatcgtacaa
     4201 gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag atgcccagtc gatccgctac
     4261 actacaaata acccgaaagc aaagggaatc tttacaaata aataatataa aaactaagca
     4321 aattgctcac tcgcagatat ttctctgtga gcagcatctt gcttagtttt tttgattcat
     4381 tgagatcaaa tagtgaaccg attcgtatga taaaattgcc ataaatgaaa tgtctgagtg
     4441 agggccatga tcaaaaataa aatggaaacg atcaaaatcg aatgggcgcc aaacagatga
     4501 tgacagaaaa caaggacaat agctcctgcg ataaaggcaa gaacgacact tagatagtgg
     4561 aaaaatcgat caaaatctgc tttttcttta gaaatacaat aatcatataa gttcgttgct
     4621 acccctttta aatttccagt tgtaaataaa ttagtgtagg cacgcccgtt gattttatca
     4681 aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa tcgttactaa tgcgggatgc
     4741 tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg cttcataata tagaacaaac
     4801 aagcgccaga aatgtttttc tttttttcta aaataatcaa ttagatactt tgttagaaga
     4861 atccctaaga aaaagaaaag ggtagacaat acttttttac caacacttag ccattctccg
     4921 tcaaatgctt ggatgatcga tagaatgata ttgccagttt gagcagaggc aaaagcacca
     4981 tattggatat aagtataact gtccatggct cctccgataa atgtcagcaa aagtcctatt
     5041 gtacgcccct cgtgaaagga agtctgcatt tttttcatca ccttttatga atatttttgt
     5101 cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa ttttcagaaa actaaaatca
     5161 tatcgcttcc cattttcatt tttagaaagg tctgttagta taaagaaaaa atagacaacg
     5221 aaactttaaa taaccaactt gaaataaaca aattcaaaga agaaagatgg gaaggaaatc
     5281 aatcagttga atgaaaaaga aattctccga ttacaaaaag aattgaaacg ctgggagaac
     5341 aagtataaaa agcttcaaca agtctttcgg ctacaagaag aaatcattgc tacctatgaa
     5401 gcggcttatg gcgaagcacc cggacttatt gaggaagaag tacaagtaaa agaaagtgaa
     5461 ggaaacagct ctttctttaa aatgatggag aaacgttcct ataatcgctt caatcaagaa
     5521 caaaaagcag ctatcacata tgatatgaag aaacatcttc gaatcatagc aggagctggg
     5581 agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc tgatgacgca aaaacaagta
     5641 gacgaagaac gcatactaat gatcactttt acaaggaacg ctgcaaatga actaaaaaaa
     5701 cgagtagata atttcagcca gagaaaaacc gctgtccata tcggaacatt ccatagtatc
     5761 ttttttagga tatacaatga aatttgtcga aaatttcctg aagtagcaat gcaaggaata
     5821 caaggagact tttcaaagga tactgcacaa aaagtgaatg ctgttttgca acagctgata
     5881 cgtaaatata atctttatat atttgatcaa tatggagaaa aaacgattgc ttctcggtta
     5941 gactattggc agaatatgaa cctttcagta gaagaaatgg ttcagttagt taaagaaaaa
     6001 tatgattcca ttgataagaa tgcacgacag ccgatatcag aacgactgta tggattgctc
     6061 acggaactcc aagaacaaaa aagaagccag caattgctag agttcaatga tgtactccaa
     6121 aatctgaaaa gcgctttgga gaatgaggag atccagcatt atatcggcca aaaatatgat
     6181 tatctattca ttgatgaatt tcaagatact aatccacttc aatgggaaat cgtgaaacgg
     6241 atgacaaagg aaaacaggat caagttgatt gttgttggag atgatgatca gagtatctat
     6301 ggattcagag gatcagagcc agcatacatc aagaactttg aaaaagaata tcctaccaaa
     6361 actcttttct tattgactaa ttatcgttct cgggcagcta ttgtccaagg agcaaataga
     6421 ttgatcagct ataataaaaa tgatcggata ccaaaggcga tgattccagc acaaaaagaa
     6481 gcaggtgtta tggaagccta tttgttttct gatactaaag cagaaagtca gtggctaatc
     6541 aaacaaattc agacatttat tcaaaaaaat gggaaatata aggaatcaat catattatac
     6601 cgatcagctt cacaaacgca gcagcttgtc caatctctgc tgaaaacgaa tattcctttt
     6661 gtactggaag cagatagtcc atatgaaggt atctttggta tcaagtcttt ccaacatttc
     6721 tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc aaaagaaaaa tcaggcctac
     6781 cagcaattgc tccggcagct gatggcagac tgctacctga aaaaaagtga atgcgatcaa
     6841 tacttttctc ctaaatatca aaatatagcc gttgcagatt atattttgtc gataagcccc
     6901 aatttaaaat cgaaatctgc agaaatccga gagtttgagg aagcgttgcg taaaacagac
     6961 cagcatcaag aaatctattc gcttgttcaa agctatcttc atctgccacg tatagctaag
     7021 gaaatcgacc agagtgatca agaatggata cgagaagaag taaagcagca tgctactttc
     7081 agaagactcc aagccttatc gcaagaaacc caagcgacag ctaaagaatt aaaaacccgc
     7141 ttgattgctt acagacaagg aaaacttgat gcactatgta tccagagcat tcacaaaagt
     7201 aaaggtctta gttaccgaaa tgtctttttg attggttgta acgaaggaag tttgccttat
     7261 aacggagcga cagaaaagaa agtaatcgac tttaatgaaa taaaagcaga accagtgacg
     7321 acgatagaag aagaacgaag actattttat gttgcgatga ctagagcaag aaatcgtctg
     7381 tatctatgtg tacctcaaat gaaaaattca agaaaattga aagtatcgag atttatcaaa
     7441 gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga gagggtgtga cagaagtggt
     7501 cagtttcaag aaagaagaag gaatgtacga aaattgcttc tcaaattttt gtgaaatttc
     7561 agcttatttt cgaagaagct atttctggaa ca
//
LOCUS       DARBEG010000108         7461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000108 DARBEG010000000
VERSION     DARBEG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.110"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(804..1217)
                     /locus_tag="SCK20_002480"
     CDS             complement(804..1217)
                     /locus_tag="SCK20_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG1146504.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1673..2158)
                     /locus_tag="SCK20_002481"
     CDS             complement(1673..2158)
                     /locus_tag="SCK20_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146505.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2297..2512)
                     /locus_tag="SCK20_002482"
     CDS             complement(2297..2512)
                     /locus_tag="SCK20_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG1146506.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2736..3536
                     /locus_tag="SCK20_002483"
     CDS             2736..3536
                     /locus_tag="SCK20_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1146507.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3555..5423
                     /locus_tag="SCK20_002484"
     CDS             3555..5423
                     /locus_tag="SCK20_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1146508.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5416..5907
                     /locus_tag="SCK20_002485"
     CDS             5416..5907
                     /locus_tag="SCK20_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG1146509.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5895..6449
                     /gene="srlA"
                     /locus_tag="SCK20_002486"
     CDS             5895..6449
                     /gene="srlA"
                     /locus_tag="SCK20_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG1146510.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6467..>7461
                     /locus_tag="SCK20_002487"
     CDS             6467..>7461
                     /locus_tag="SCK20_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG1146511.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2652 a         1198 c         1422 g         2189 t
ORIGIN      
        1 attttttatt caaaaaaaat agcacaatgg caaagatcgt aagagagatc ataagcccca
       61 atgaatgact aaaaagaaaa gcagaatcac atgccatatc atcccatatt tcttcaagaa
      121 aataaataag cgggaaaaag ggaaggaata gtagaacatc gatccagcac catttttttc
      181 ttcttttgaa ataacataaa acaataaaca tgaacgtgca gggaagtaaa aacaaagctt
      241 tactcataac taacaaaata ccagctcctt ttattttttt ctcccccaca ttcaaagagt
      301 aacaatggta tgtacttaat aatagaaaaa tggatcaact tgtatgaata acagctgaaa
      361 agatctgcac caaataaaaa acttccagcc aaaactgact ggaagtttat ttttttctga
      421 aatattctaa cacaaatatg aaaaagatgg ccgaagctaa taagccgatt tcgtagcaaa
      481 acaatgagat accagagtcg taataataaa atcctctaat cttatggata aaatagatca
      541 tcggacaagc agataagagt aagtatttgt taggcctttt gaataagtaa aagaaagcac
      601 tggtcattat gataaatggt atccaaaaca aaagtttact taagacaatc aaatgatcaa
      661 ctcccatatt ttttaccaag agagatcata acatctttat cgtaaaaaca aaatattttt
      721 ttgctcattt tgaaaattta aacatactcg ttcttatcca aatatataaa aatcgcccct
      781 cattcatcga gaggcgataa gttctaattt aactcaattt tttttgtttc ttttggctga
      841 taagctgttt taggtaatac caatttcagc acaccgtttt taaaggttcc agtgattttt
      901 tcttcatcaa cattaggaag atagaatgaa cgtttgtaag agaccgaatg tctttctttc
      961 cgtacatagt tgccatcttc tttatcttct gtatgggact catgattggc ggagatcgtc
     1021 aaagtattat ctgaatattc aactgtcgtg tcttctttgt cacagccagg catatcagca
     1081 gttagtttgt attctttttc attttcgact aaatctactt ttggataact tgaaacacct
     1141 aaaaaatcat tgaatgctgg actaaacacg tcattgaaat cagggaacat gtcgcgaatg
     1201 ctaggtacat ttgccatttt tgatcatcct ttcaataagt attttttatt tatccctatt
     1261 tacgtaataa ctctattttg aaaaaaatgc aagtataatg gttagctttg tgaatatttc
     1321 atctaatacg atacaaaggc tatctttcta atttgaaaaa gggatcatta aaataataag
     1381 aagaaaatat gtcaaaaggt aaaaaagtaa attcttctat ccaaatatag aaaaattgaa
     1441 tctagaaaac cgcccgacaa taaatttcat taactcagtt gaccgaaaat gaagtagata
     1501 ccaaaaattt atcaagagag caagtagaga agtgagtata ttcataagag aaatagctgt
     1561 aacgaattat taggttaatg caaatgctta tttatactcc gtataacttt ctcagaaaaa
     1621 atgataccaa taatgacttg ctagtactgt aaatccgtat aatatcatct aatcaaaatg
     1681 gtgttccatt tgcatctgtc atcgtaatcg atccttcggg tgacactcga taaaaaccaa
     1741 cgcttccaga tcctccttgg gaacggattt gtttgctgga agctgtgaac aaataatctc
     1801 gatcaatttt ttgataaaag ctaataacaa tatcgtcact ttgtaattcg gaacggtggg
     1861 caataagaaa tagaagtgct ttgtcagagt tttctaatgg aagagaaggt acagtgcttt
     1921 cttgcatttc agatgttgaa gaaatgctaa acgactgaat acttgattgg ctatgttgcg
     1981 aatctacatt accagtagaa gtattagtaa tggaagtagt agaagtactt ttagttgaac
     2041 caacctcagt tgttgaaatg gctgctgaag attctttaac agaattcttt gttttatatc
     2101 cacaggcaga aaaaagtatt acagttccca acaagcctgc gtaaatccct ttatgcatga
     2161 tagctcctcc taaaatagat tatctttatt cagtatatca cttaaacaaa gaaaagcaga
     2221 tggtttaaaa tgaaaaaacc ttcccaataa agggaaggtt catgaagata ataacttttt
     2281 taataattat agctttctaa tatctttcat caacttttct agtaatcatt gggaaaaatt
     2341 tcaagcagta gatcgcacaa atacctacga cgatataaat gatcttcgca aaaatagttg
     2401 ttgaaccacc agcaatcata gctactaaat caagttcgaa caagcctacc aacagccagt
     2461 tcaaccctcc cacaatcaat aaagttaatg caatagcatc taaagttttc attagaattc
     2521 tcctttctta aatgataaaa caattatata tcaaaaagag aaacattgct tatataacgc
     2581 tcaggaattt tagcaatcta tgcaaaaaga tgaaaatttt ttttgctctt ccattagtac
     2641 acatttttta gctgaagctg taaatttcat gtcttaattt tttttagtga gcttttatac
     2701 tggatttgta agaaaaaaga aggagaggat cacatatgtc ggattggttg gatattgcag
     2761 gaaaagtagt aattgtaact ggaggttcct caggaattgg aagaagtatc gtcgaaaatt
     2821 tgttgaagca gaatgcacaa gtagcgaatt ttgatgtaac tgaatgccgt atacagcatg
     2881 aaaacctact atctttaaaa gtcgatgtgt cttctaaaac ggacataaaa gaagggatat
     2941 ataaagtaat gaaacatttt aagacgatcg atggtctagt caataatgca ggaataaata
     3001 ttccttctct tctaatagat agaaatcatc caaaaagcaa gtatgaatta tcagaacaag
     3061 tttttgataa gatgatcgct gtcaatcaaa aaagtgtata tttgatgagc caagcagttg
     3121 gaagaatctt ggtgcaaaag ggatcaggag tgatcgtgaa tctttcttct gaatcaggat
     3181 tggaaggatc agaaggacag agctgttatg ctgctacaaa agcagcgatg aacagtttta
     3241 ctcgttcttg ggcaaaagag ttaggaaaga ggaatgttcg tgtggtaggt gttgcaccag
     3301 ggatactgga agaaaccggt ttgagaacac aagaatatga agaagctctt tcttatacga
     3361 gaggtataag cgtcgaacag ctgagaaacg gttacagtcg aacaagtacg atccctctag
     3421 gaaggagtgg taagctgcaa gaagtggcgg atctagtctg ctattatctt tcagaaagat
     3481 caagttatat cactggagtc acgactaata tttcaggagg taaaacaaga ggataaagga
     3541 taggggggac gcacgtgacg ctagtaaaca gatggtacca attattgaaa ttactagtca
     3601 ctcacaagaa agtttcaatc caggaagtgc aaaaagaaat caaaaccagt aatcaaacca
     3661 taaaaaaaga tattggggag ttgaatgaag aaattgccgg tattgcaaaa atcatccaaa
     3721 aaaacaaaca ttttgaactt gaaatttatg attttgattt ttttgatgag atcatgcaag
     3781 gaaaattgaa aacagccagt gatttcaatt cggcatccaa aagaatcgct tatttactaa
     3841 ataggctgat cgaaacagag gatttcttac gtatggatga tttgtctgaa gaagtcggtg
     3901 tcagcagagg aacagtggtc aaggatctaa agacattaaa agagatgatc aatgattttg
     3961 atgtaaagat tacaggcaca ccaaataaag gactaagaat aataggagat gagctagaat
     4021 tacgactact tttattttat tttgtttctc cctacttttc agagacgaaa acagaacagt
     4081 ttctagaaga aaaacaagca atcaaacatt ttcagaagaa gacgaatgct agtaaacaag
     4141 aaatcaccct tttaacgaaa gttatcggta tttctttgaa tagaatctct agtaactatc
     4201 tgttgaagaa gcccatcaat cgttacagtg ggtgttttga atatatccaa gaattcaatg
     4261 aactgatcaa tcagttggaa gaaatctatg aactaacttt gagccaatat gaaaaagatt
     4321 ttatcagttt tcctttgaac atcatcaaca caagtataac gatgataagg aaagctaact
     4381 ctgatttgcg cttatatttc gaccccatga tgatgcgtat tcgacgatta gatctcattg
     4441 atctggatga gtccttctta tatcaagaga tgaaacagca cctctcttta ttgataaatc
     4501 gggtaatttt tcgttatcga ttgactgatt tattttatgg tgagatagaa aaaaagtatc
     4561 ctttttctta ccaaatcgct agcgaatgca tacaagcatt agaagctatc ttgcaacata
     4621 agatctctaa agtagagatc agctacctaa cgttgtattt ggagttagca tttcgatatc
     4681 aaaaagagca tttttcctct aaaaaagtag ccattgttca tacgacagga aaaggaacag
     4741 cattaatgat ccaaagacaa atcaaaagga tattggggag ggatatacaa ataacacatg
     4801 tttcagaaga acaatatgaa aaagtagatc taaacaaata tttcgctgtt tttacaacga
     4861 ttccgttaaa aaatgtaaat gaagagacac cggtgatcca tttgactaat ctttttgatg
     4921 actcttggtt aagggcacaa tggcaaaaag caaaaaaaaa gcgattagcc gggtttcatt
     4981 ctactgtttt tttctatcaa atatggaaag aaaaaaagac gtatcaagaa tatttaaaca
     5041 aattagtcgc tcctttgatc agaggctatt atgtggatga agaatttgaa aaaaggatct
     5101 tgaaaagaga atatcagcaa agcacatttg ttaacaaaga atttgctttt cctcacacaa
     5161 ccaaccagct atctcaacac attgttttat cagtagggct aataactgat caaggtttag
     5221 cagaaacagg aggaaatgga gttatttttt tatttgccat acctgaagaa atgacggctg
     5281 aagttgagaa tgaattattt gagctatacg atatcctatt tgaactcata aaaatgggaa
     5341 tagatgagtt tattgaatta gcaccgacac aagaatcatt tttaaaacta atcaaaagta
     5401 aaggagagag acttcatgaa tgatatgtat ctattcatag ttttcgcagc ttcagcttat
     5461 gtgctgcaag cagttatcag ttggaagcaa ctaaagcatt tcaatcaaac atatagcgaa
     5521 ctgcgaagaa aaggaaaagt agcgattggt aaacgacctg ggaaaatccg ttcaggcacg
     5581 attgttatgc tagctataga cgatcatgat caagttttag aagtcagaaa aatgcaaggg
     5641 gttacatctt tggcaaaatt tcattcatta cctgtctatg cgggtgaaga tatccattac
     5701 atcgatcgtt accatccctc agttcgagcg gaagatcgtt tgacacaagc agctttagaa
     5761 aatgccagag aagtatactt acgaatagag gcaggaaatg aaaaaagtga acaaccttca
     5821 ttgcctttta ttgctatgct caataatttt cggatatgga aaagtacgat caaagaaaaa
     5881 aggagtgaca aacgatggat atcataacaa aatttgctga gggatttatg aggctgttcc
     5941 aaacaggtgc agaaacattc ataagctgga tgacaggaat cgtaccagtt gtcttgatgc
     6001 tgatggttgc aatgaatacg ctgattgctt ttttaggtga agaccgagta acgaaagtag
     6061 ccaaattatc agcgaaaaat ccgcttactc gttaccttgt gttaccgttc ttatctgctt
     6121 ttatgttggg aaatcctatg tcatttacaa tggctcgttt ccttccagaa tactacaaac
     6181 caagctatta tgctgctcaa gctcaatttt gtcatacgag caatggtgta ttcccccata
     6241 ttaatcctgg agaattgttt gtatggatgg ggattgctca agggatcgaa accttagggc
     6301 taaattcgat ggagctggct atccgatact tgcttgtggg actgctgatg aactttattg
     6361 gtggttggat aactgatttt actactggat ttgtatgcag acagcaagga atcgttctta
     6421 gcaaaaaagt agagctttca gttgattaga gaaaggagag ttttaaatgt atggtgatat
     6481 taagatcgta agaggaaacg gcgggtatgg aggtcctttg attattaggc cgacaaaaga
     6541 taaacacaag ttcatctatg tcacaggtgg aggtgagagg ccaatgatcg tagataaaat
     6601 agaagaactg acaggtatgg agtctatcaa tgggttcaaa acatcaatac ctgatagtga
     6661 aatcgcgctt gcaatcgttg actgtggagg gactcttaga tgtggaattt atcctaaaaa
     6721 agggatacca accatcaata tcgttcccac aggaaaaagt ggcccattag cacaatatat
     6781 cactgaagat atttatgttt cagcagtagg gttagaacaa atacagccac taatggaaac
     6841 tgatgataac aaagaaacag taaatactgc atctccagat aaaaagtaca aatatgatac
     6901 ttctaaaaag ataaccgagc aaaaagcaga gcagcaaaac tggatagcaa aaattggaat
     6961 gggcgcggga aaagtagttg ctacattcaa tcaatcagca agagaagcag tagatacgat
     7021 gatccggaca gtgatccctt ttatggcttt cgtttccctt ttgattggtg tgatacaagg
     7081 ttctgggatc ggtaatgtat ttgctaacat tatggcacct ttagcaggaa atgcagttgg
     7141 attaacaatc attggcttta tttgttcact gccattttta tctcctttgc ttggtccagg
     7201 ggcagttatt agccaagtga ttggaacatt gatcggtgta gagattggga aaggaaatat
     7261 tcctccccaa ttagcattac cggcactatt tgctatcaat actcaaaatg catgtgattt
     7321 tattccagtc ggtctaggtt tggaagaagc aaaagctgag acagtagaag taggtgtacc
     7381 atcagttctt tattcaagat ttcttaacgg catcccacga gttatcattg cttgggtagc
     7441 aagttttgga ttatatagct g
//
LOCUS       DARBEG010000109         7373 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000109 DARBEG010000000
VERSION     DARBEG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.111"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            114..506
                     /locus_tag="SCK20_002488"
     CDS             114..506
                     /locus_tag="SCK20_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146512.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(679..861)
                     /locus_tag="SCK20_002489"
                     /pseudo
     CDS             complement(679..861)
                     /locus_tag="SCK20_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            848..1045
                     /locus_tag="SCK20_002490"
     CDS             848..1045
                     /locus_tag="SCK20_002490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146513.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(1088..1663)
                     /locus_tag="SCK20_002491"
     CDS             complement(1088..1663)
                     /locus_tag="SCK20_002491"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1146514.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1818..2344)
                     /locus_tag="SCK20_002492"
                     /pseudo
     CDS             complement(1818..2344)
                     /locus_tag="SCK20_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2801..4312
                     /gene="gadC"
                     /locus_tag="SCK20_002493"
     CDS             2801..4312
                     /gene="gadC"
                     /locus_tag="SCK20_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG1146515.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4325..5725
                     /locus_tag="SCK20_002494"
     CDS             4325..5725
                     /locus_tag="SCK20_002494"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG1146516.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5890..7330)
                     /locus_tag="SCK20_002495"
                     /pseudo
     CDS             complement(5890..7330)
                     /locus_tag="SCK20_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         2328 a         1225 c         1312 g         2508 t
ORIGIN      
        1 tttaagtaaa aaatataggt gatttttttt gacttcatat ttataataaa gacacgtaaa
       61 tcaattattt ttcattttac tttaactaat aatgtttatt tggagggatt attatgttag
      121 atgatagatt gacgtgttct aattgtggta cattatttta ctatgaggaa gttagaaata
      181 ttgtaaaaca tagaaaaaaa gaaatgccta tacgttgtcc aaattgcaat gaagttatta
      241 agcgaaagtt gtctcatggt tattttgtca gctacgaact atatgaagaa agtaaaataa
      301 atctgaaaaa aaaaccaaaa tactataaaa atttgaaaac tggagctatt ataactgaaa
      361 aagaatttaa aaatttagtg actgaagaag aaagcaggtt ttataatttg tctggtaata
      421 tttgccctgg atttatggat cagattattg aaaatgagtc aattgaatca attgaaaact
      481 atgttcctgt tgacttacca aaatagaaaa aataatcctg tttttacagt gaattatttt
      541 aattttgatt ttcaataaaa atatattaca ataataatcg taatagctaa tgaaacaatt
      601 tctcaatctt tttaccactc caaacaggga gcgtcaataa ttttgtgtaa ataactcgtc
      661 cttctgcaaa ataatgggtt actcagtaaa cattgaagct aatgtatccg tcacttgttg
      721 gaagccttta tggcttctat ttaagaattt ttgattgtat gtatcaaaga tggatactag
      781 gaaacgctct agtgattctt cattttgaaa ctgctctttt ctgcggctgt atttcttgat
      841 ttgtttattg aaagattcga ttgtagaacc agaaggaatg gagaacactt ttgaacttta
      901 caacaaaact actgttgata aattaattcc aattttagtt ccaactgatt taaatggtca
      961 tgatgtttta cttcgtttta acggattttt aggtgaggat tatagatacc taaaaatttc
     1021 tgatcctcaa cctttaacag aataataaaa aacgtgtaat caattatttg attacacgtt
     1081 ttttatttca atcttcgttg tagtagtggt tgcgacatac cgaaaggtaa tcttcattac
     1141 ctcctactac aatctgttca cctgaataaa ctggaatacc atcaacaata cgagcattca
     1201 ttacagcctt tttattgcaa aaatggcaaa tagttttaat ttcctcaatt ttatcagcaa
     1261 atgtcaataa tgcttcggat cccttgaaga gattattttg aaaatcattc ttcaatccat
     1321 agcaaattac tggtacttcc tcattcatta cgataagtga tagatcaata atattctctc
     1381 tacttaaaaa ttgagactca tcgaccaaaa tacaatcaac ttttccgtac tgatcaataa
     1441 tctccactaa ttctccgtac acatccattt cagaagtaat tgcaatagct ggtttcgaaa
     1501 cacctatacg actctcaact tttccaaatc ctgatcgagt atctattgcg cttgtcatta
     1561 tgataactct cttcccttgt tcttcataat tatgagccac tttcagtagc tcaatactct
     1621 ttccactatt cattgttcca tatctgtaaa ataattgtgc cattaaattt attcccttct
     1681 tttacaattc aattattcct taattccatc tgcttttatg cagctgctcg tttattcctt
     1741 gatatgtcta taacatctac tatccctaaa accaatttat aagccattag agatggtatt
     1801 cctgaacatc tctcatattg ttttttgagt ggtatatcat ctgaaaaact gaaggaatca
     1861 tgaaaatagc gacatactta tcatttagag ttctaacttt aaatgcattt acttcttcag
     1921 tcagcacttt agtcatgttg gacatggttt ggggcgtgta gtgatgccca tacattttct
     1981 caatcaagtc tgcgatttcc gacatggtaa taccttttcg gaagaggtga atgacggtct
     2041 cctctaatgt gtcattcgtc cgtctataag caggaacagt ctgttgcttg aactcgccat
     2101 tgcggtcgcg cggaatctgg agatgaagtt ccccatactc ggtcttgacc gtacggtcat
     2161 aggagccgtt acgagaatta cccgtgttaa aaccaatgcg atcgtacttt tcgtaatcga
     2221 ggaaagccgt taactccgtt ttgagaagcg tattgacagc tttttccaaa tggacacgga
     2281 ataatccatt caaatcgcct ttgttcgcta gagtctttag gatttctgta gtaaaatcgt
     2341 tcatatggaa gtcctctttt ctgtgaatgt gttgtggtaa ctctattcta cagaagggac
     2401 ttcctttttt ctatttacac aaaatatttt acactctcaa caaaagtatc ttcgattaat
     2461 acttgctcaa gttatgtgca gctacgaaaa agtaggtacc gaggtacttt ctttttcgca
     2521 gctttttttg gacttatgag aagtacattc gctggaagaa tacgatataa aacgagcttt
     2581 ttcactaaag atattaatag agtgaaagaa acaagctttt gttgtcagtt ttttcacttt
     2641 tctaaaaatg aaaataaaaa attctggaag tggagaagaa ttttgcttgc tatactttgt
     2701 cttgtagaaa agaggtggac aaaataatgc aagctcaaat aagcgatttt ccataacctt
     2761 ggtaggaatg acaaattaaa taggaaaggg aagtaataat atgaataaaa aaaagttatc
     2821 tctattcggt ttttttgccc tgacagcttc catggttttg actgtttatg agtatccgac
     2881 ctttgcaaca tcacaattac atttggtttt ctttcttctt ttgggaggta ttttatggtt
     2941 tctaccagtt gctttatgtg ctgcagaaat ggcaacagtt gaaggatgga aagatggtgg
     3001 gattttcagt tgggttagtc aaactttagg agaacgtttt ggttttgcag ctattttttt
     3061 ccaatggttt caaattacag taggttttat tacaatgatc tacttcatac taggagcgct
     3121 gtcgtatgta attaattttc cagagcttaa tacagatcca tttatcaagt ttattggtgt
     3181 attagtcatt ttctgggtac tgactttttc acagttgggc gggaccaatc gtaccgctaa
     3241 aattgctaaa gcaggatttg tcattggaat tgttattcct tcgatcattc tctttggttt
     3301 agcagcagcc tactttatcg gcggtaatcc gattcaaatg gctattaatg cgaaagcttt
     3361 tattcctgac ttttctcagc tttcaacttt agtagtattt gtatctttta tattggctta
     3421 tatgggcgta gaagcttcag catcacatat taatgagctg gaaaatcccc aaagaaacta
     3481 cccattggca atgtttttac tagtgatttt ggccatcgcc ttagatgcaa tcggcggttt
     3541 ctcagtagca gcggttattc cacaacatga cttgtctctc agtgctggtg taatccaaac
     3601 atttgaaacg cttattttac attttaacag tcatttacat tggttggtaa aagtgattgc
     3661 tctcatgatt gcctgtggcg ttatgggtga agttagttct tgggtagttg gtccatcacg
     3721 agggatgttt gcagctgctc agcgaggtct gttaccagtt ttcttccgga aaactaataa
     3781 acaagacgtt ccagttccgt tgattatgat acaaggaatt atcgttacta tttgggcagc
     3841 agtcttaact tttggtggag gaggcaacaa tttatcattc ttagtagcaa tctccttaac
     3901 tgttgtcatt tatctcgtag gatatttact atttttccta ggttactttg tattagtttt
     3961 taagaaaaaa gacttaaaac gaacttacaa tgtaccagga aaaactgttg gcaaaacgat
     4021 tatcggtgga attggtttta ttttatcagt gttcgcattg cttatctctt ttgtaccacc
     4081 agcttcaatt gcagctagtg agcaccgaac ataccaaatc atactactta ttagctttgt
     4141 agtgactgtt cttttacctt ttatcattta tgaagtacat gataaaagtg ctcacacaac
     4201 tattgaggaa cctactcacg tgaaagctga agacgttaat cccgctgttt atccagctgc
     4261 tagaggagag caccacgtta caaaaaatga tgaacatatt ttaaaacatt aggaggaatt
     4321 cattatgtta tacggaaaag ataatcaaga agaaaaaaac tatttggaac caatttttgg
     4381 ctctgcaagt gaggatgttg acttgcctaa atataagtta aacaaagaat ccattgaacc
     4441 acgaattgct tatcaattag tacaagacga gatgttggat gaaggaaatg cgcgattaaa
     4501 cttagctact ttttgtcaaa cgtatatgga acctgaagca gtgaaattga tgacccaaac
     4561 gttagaaaaa aatgcaattg ataaatcaga atacccacga acaacggaaa ttgaaaaccg
     4621 ctgtgtaaat atgattgctg atttatggca tgctccaaat aatgaaaaat tcatgggaac
     4681 ttcaacgatc ggctcttcag aagcctgcat gctgggtggt atggccatga aatttgcttg
     4741 gcgtaaacgt gctgaaaaat taggtcttga tattcaagca aaaaaaccta acctggtgat
     4801 ctcttctggt taccaagttt gttgggaaaa attctgtgta tattgggatg tggaactgag
     4861 agaagtccca atggatgaaa aacatatgtc aattaatcta gatactgtca tggattatgt
     4921 ggatgagtac acaattggta ttgtaggtat tatgggtatt acttacactg gtcgttatga
     4981 tgatatcaag ggtctgaatg atttagttga agctcacaat aaacaaactg actataaagt
     5041 atacattcat gttgacgctg catcgggtgg cttttatgca ccatttactg aacctgatct
     5101 agtttgggat tttcaattga aaaatgttat ctcaattaat tcttcaggtc acaaatatgg
     5161 tttggtatat ccaggtgtgg gttgggtctt atggcgtgac caacaatact taccagaaga
     5221 attagtattt aaagttagtt acttaggtgg agaaatgcca actatggcta tcaacttctc
     5281 tcatagtgca gcacaactaa ttgggcaata ctacaacttt gtacgctatg gctttgatgg
     5341 ttatcgtgat attcaccaaa gaactcatga tgttgctgtt tatttagcca aagagatcga
     5401 aaaaactggt atttttgaaa tcattaatga tggatcagaa ttgccagttg tgtgctataa
     5461 gctgaaagaa gatcccaatc gcgaatggac actatatgat ttatctgatc gtctgttaat
     5521 gaagggatgg caagtcccag cctacccatt acctaaagac ttggatcaat taattattca
     5581 acgcttagtt gttcgagcag actttggaat gaacatggct ggtgattatg tacaagatat
     5641 gaaccaagca attgaagagt tgaataaagc tcatattgtt tatcataaaa aacaggatgt
     5701 gaaaacgtac ggctttactc actaaaattc ttcagagaaa tataaaagaa gttaattaac
     5761 taataaaagg agttgtgatt accacacagc tccttaaatc tatttgaata aatatatgat
     5821 attcatttgt tacactctca atcttagctg ttgttctatt catcattact cttattgtaa
     5881 gtagttcagt caaatttgct tgttcaattg atcgataaca gtatgaattg tttgaaaagt
     5941 aatcggatat ataattgttt tctcactacc taagccttct tggtataaag aactatcgca
     6001 aagtataatc cgctgagtac aaaagaaagt gtcgtctact atttctctaa gttgtttaca
     6061 aatgatatta ctaatagaaa agtgttctcc caaaatgtct tctaactgtt gtcgatagta
     6121 taaataccta aagtctgacg gagctactat gaacacttca attggagatt tccgtttatg
     6181 ctcatttaaa agacttaaac taatggttag tttacgaata gaattctcat tccaacgcag
     6241 aaataaattg ttttgttttc gccattgttc aagaatttca tgaacttcta agtacaaagc
     6301 gcgttgctct tcatttaaca agtaagtctt atcaggttga aatatctgaa tatcagccca
     6361 aagtgagcgg ataaacggga gaagtgcttt atcaaaaagt atctgatttt cttcagatat
     6421 attcagagat aagataagtt tttgactgag ctcaatcacc aaagggttat ctgctacatg
     6481 gttttgataa acaacgttca agtctttttg taacaattca aagttatcgc atagataatt
     6541 tctagagttg aatagaaagt agatataaag gcattcatct tcgtcatgac ataaagagaa
     6601 agaaagttgc tctaatcctt tctgtattag ctgaaacaag ggagtatgtc gggcaactct
     6661 tttttcttcc tgactaaaat atattgggtt agtcttagat ctctgcaaac cgatagcgat
     6721 acctaataaa ataaattgtt tttcaactgt agggtaactt cttgaaaaaa aatgtttttc
     6781 tacataatca atcaattgtt gaccgctttg aataattgat ttatttagct ggatcaactc
     6841 tgtccgattt gtctcataga ttatcgttag taacaaactt cgataatcct tttcaggaat
     6901 ttcaaaatgg ccatcagcat ttaagtactg catttgttta aaaaaattaa tcaactcatt
     6961 tcgtaagttg taaactttgc ttaacgaaat gaaggtagta tcagctatat tactccaatt
     7021 gtgttcccct gataaataat gtcctagtac attgaaaaag tcagagcgct gatagatgag
     7081 ttgagtgagt gccaacctcg aaaacgaaga gtcgaatgcg actgtaaagc tacttctaga
     7141 tttctccact gaaataattt gctgatggag tatgtcggta atgcattcca agtcgttagt
     7201 gacagacagt gagtgggcaa acgttcaatt gatctgctat ttcgcgaatc tgaatggtcc
     7261 ctttttcgtg taattgctca caaatgtaaa cttgacgaaa aatacttttc tccagatagg
     7321 tttccaacat agtactcctc ctagtcattt tatacataac tattataact cta
//
LOCUS       DARBEG010000110         6861 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000110 DARBEG010000000
VERSION     DARBEG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.113"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCK20_002496"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCK20_002496"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG1146517.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCK20_002497"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCK20_002497"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG1146518.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SCK20_002498"
     CDS             complement(2303..2965)
                     /locus_tag="SCK20_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1146519.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SCK20_002499"
     CDS             3149..4795
                     /locus_tag="SCK20_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1146520.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SCK20_002500"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SCK20_002500"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1146521.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..>6861)
                     /locus_tag="SCK20_002501"
     CDS             complement(5976..>6861)
                     /locus_tag="SCK20_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEG1146522.1"
                     /translation="ICKENFPATSVKDVQKEFTEYEYIDFFIDLSVDILRPNLPICRE
                     QRILVNILARLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESLCTVFDLFIPEK
                     EKILYSLAFARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQN
                     QHLLPVILEKENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFS
                     NESIKIQNDFMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFE
                     LKEEALMDQSKKENQVTS"
BASE COUNT         2129 a         1274 c         1287 g         2171 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat t
//
LOCUS       DARBEG010000111         6762 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000111 DARBEG010000000
VERSION     DARBEG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.114"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..182)
                     /locus_tag="SCK20_002502"
     CDS             complement(<1..182)
                     /locus_tag="SCK20_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1146523.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="SCK20_002503"
     CDS             517..951
                     /locus_tag="SCK20_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1146524.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="SCK20_002504"
     CDS             974..1294
                     /locus_tag="SCK20_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146525.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="SCK20_002505"
     CDS             1435..1626
                     /locus_tag="SCK20_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146526.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="SCK20_002506"
     CDS             complement(1697..1954)
                     /locus_tag="SCK20_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG1146527.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="SCK20_002507"
     CDS             2112..2375
                     /locus_tag="SCK20_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146528.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="SCK20_002508"
     CDS             2399..2587
                     /locus_tag="SCK20_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146529.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="SCK20_002509"
     CDS             complement(2670..2855)
                     /locus_tag="SCK20_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146530.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="SCK20_002510"
     CDS             3070..3441
                     /locus_tag="SCK20_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1146531.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="SCK20_002511"
     tRNA            complement(3591..3663)
                     /locus_tag="SCK20_002511"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="SCK20_002512"
     CDS             complement(3709..4137)
                     /locus_tag="SCK20_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146532.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="SCK20_002513"
     CDS             complement(4197..4955)
                     /locus_tag="SCK20_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG1146533.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="SCK20_002514"
     CDS             5250..5447
                     /locus_tag="SCK20_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146534.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="SCK20_002515"
     CDS             5553..6035
                     /locus_tag="SCK20_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146535.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="SCK20_002516"
     CDS             complement(6093..6689)
                     /locus_tag="SCK20_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1146536.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2282 a         1047 c         1334 g         2099 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aa
//
LOCUS       DARBEG010000112         6644 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000112 DARBEG010000000
VERSION     DARBEG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.115"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..859)
                     /locus_tag="SCK20_002517"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="SCK20_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="SCK20_002518"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="SCK20_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG1146537.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="SCK20_002519"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="SCK20_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2603..3421)
                     /locus_tag="SCK20_002520"
     CDS             complement(2603..3421)
                     /locus_tag="SCK20_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1146538.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="SCK20_002521"
     CDS             3582..4271
                     /locus_tag="SCK20_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1146539.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="SCK20_002522"
     CDS             4285..5787
                     /locus_tag="SCK20_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1146540.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="SCK20_002523"
     CDS             5800..6276
                     /locus_tag="SCK20_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1146541.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="SCK20_002524"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="SCK20_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atgt
//
LOCUS       DARBEG010000113         6584 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000113 DARBEG010000000
VERSION     DARBEG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.116"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            149..1156
                     /locus_tag="SCK20_002525"
     CDS             149..1156
                     /locus_tag="SCK20_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1146542.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1168..2172
                     /gene="neuB"
                     /locus_tag="SCK20_002526"
     CDS             1168..2172
                     /gene="neuB"
                     /locus_tag="SCK20_002526"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEG1146543.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2169..3317
                     /gene="neuC"
                     /locus_tag="SCK20_002527"
     CDS             2169..3317
                     /gene="neuC"
                     /locus_tag="SCK20_002527"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG1146544.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3290..3967
                     /locus_tag="SCK20_002528"
     CDS             3290..3967
                     /locus_tag="SCK20_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1146545.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3957..5099
                     /locus_tag="SCK20_002529"
     CDS             3957..5099
                     /locus_tag="SCK20_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG1146546.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            5112..6089
                     /locus_tag="SCK20_002530"
     CDS             5112..6089
                     /locus_tag="SCK20_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1146547.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6089..>6584
                     /locus_tag="SCK20_002531"
     CDS             6089..>6584
                     /locus_tag="SCK20_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1146548.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGG"
BASE COUNT         2446 a          823 c         1252 g         2063 t
ORIGIN      
        1 aattttttct ttgatacttc atattttaat caatcaaatg ttgattcgtt acgttttgac
       61 atcattattg ataattagtt tagttatata ttttttaaag attaagaatc gaattataga
      121 tgactataat tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg
      181 cggaactggt tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc
      241 tatcagaatt tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa
      301 tcacactaat ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc
      361 atgtcgtgat gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga
      421 atataatcct atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc
      481 agcgatcaaa aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc
      541 tactaacacc atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca
      601 gtataaagga aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc
      661 aagtggatct gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt
      721 gacagatgtt gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct
      781 gtctgctgct atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa
      841 tattttaact ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata
      901 tagaggtgaa ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat
      961 gactacagac gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca
     1021 ctatcatgct actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc
     1081 ttcagatgca acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa
     1141 tgtcgtgact tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt
     1201 ggtgtaaacc ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa
     1261 tgtggagcta acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag
     1321 gctccaatgg caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg
     1381 ctaaaaaaat tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa
     1441 aataaaattg attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa
     1501 attggtgtac aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga
     1561 aaaatagata tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag
     1621 gtcagtgagg ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca
     1681 aattatccgg ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg
     1741 ttcgggatac ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct
     1801 gttgcgatgg gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt
     1861 ccagatcata aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga
     1921 aacactgaaa tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca
     1981 aaagaagtat caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt
     2041 acagagaaat ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat
     2101 caatatattg gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa
     2161 gatgttgaat gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata
     2221 aacctgttgc tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa
     2281 tgcatttatc ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa
     2341 ttagtggaga agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg
     2401 tggcattaga gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat
     2461 tattactagg ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa
     2521 tcaaaacagc acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac
     2581 atgccataac gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt
     2641 taataaaaat gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata
     2701 ctattttaaa taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat
     2761 ttgaaaagaa aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata
     2821 tagaaaaaca aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt
     2881 gcattttacc taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt
     2941 taaaaacgga tattatttat attacgaatt tagagccgga ggaatacctg actatattaa
     3001 aagagtgcaa atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca
     3061 aaataccagt aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta
     3121 taaatgcaaa tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc
     3181 agtttataaa aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa
     3241 gaataacaaa aacattgaat caaatattga atgatgggga agatattaag tggattcaaa
     3301 aacaaatagc ttattaaaat atactagtga tccatacatt attattgggg ctggcggaca
     3361 cgcaaaagtc attattgaaa ccttaagatt agaaaataag ttaatatatg gattttttga
     3421 tgataagcaa aataaaatgc aagatctgaa atatcttggg aaaataaacg aattgaatag
     3481 ctgggcggat agcaaaaaaa aaatctataa atttattgta gctattggtg actgtgtagt
     3541 aagaaaaaag attataaaaa aattgaatat gccaagtgag caatatggaa tagctgttca
     3601 tccaagtgct atagtggcaa aagaaatttc aattggttat ggaactgtta tttttgcgaa
     3661 tgctgtaatt aacccagatg ctgtaattgg agagcatgct ataataaaca caggtagcat
     3721 tgttgagcat gattgtagaa ttaataacta tgttcattta tctccaggtg tatgcttagc
     3781 gggtggagta catgtaggtg taggaactca aataggaatt ggttctcaat gtatccagat
     3841 gaagaaaatt ggatcttggt gtatgatagg cgcagggagc actattgtta aggatattcc
     3901 ctcacattcc ttagcttatg gcaatccagc taaaattaag aaagaaggaa ttaattttga
     3961 attctagaat tttattatca agtcctcata tgggaggaac agaagaaaaa tatgttgcta
     4021 aggcttttaa agataattgg atagcgccat taggtgaaaa tgtggataaa tttgaagaaa
     4081 gtattacaaa atatactggt tctaagggcg gagcttctgc gttaagttca ggtacagctg
     4141 ccatacatct tgccttaaga ctgcttaatg ttggaaaaaa cgatattgtc tttgctcctt
     4201 catttacttt tatagctaca gtaaatccta ttttatatca aggggctgta ccagtgttca
     4261 ttgactcaga acctaataca tggaatatga gccctgttgc actagagagc gcattagaag
     4321 aagctgaaag aaacaaccaa ttacctaagg caatcatcgt agtgaattta tatggtcaaa
     4381 tggctgatta tgaacgaatt ttagctattg caaaccaatt taatgttcca gtgatagaag
     4441 atgcagcgga atcgttaggt tctacttata agggaatttc gtcaggctta tttgggacaa
     4501 ttgggatcta ttcatttaac ggtaataaga tcatcacaac ctcaggtgga ggagctctag
     4561 tgtctagtga taaaaaatat gttgagcatt caagattttt agcaacacaa gcaagagatc
     4621 aagcaccgta ttatcaacat agcgaaatag gttataatta ccgtctaagc aatatttctg
     4681 ccggtatcgg acgtggacaa atggaagtat tgaatgatag agtagctaaa cgaagagaaa
     4741 ttttcaatcg atataaggaa gctttttctg gaattgatag aattgaaatg atgccagaag
     4801 tggatggcat ggtgccaaat cgttggttgt ctactttaac gatcaatgat tataattcta
     4861 ttgatcaagt agttaacgta ataaataaac taaatgaaaa aaacattgaa acaagagctt
     4921 tgtggaaacc actacatttg caaccagtat ttcaaggggt aaaaatgtat taccatgata
     4981 ataatcttag tgtgtcagaa aagttattta atagcggctt atgcttacct tctggaagta
     5041 atatgacaga tagtgaacaa gaatttgtta ttgatgagtt taaaaaaata ttcaagtagg
     5101 tgtgatattt tatgaacata ggaattattg gtttaggttc catggggaaa agaagactta
     5161 gactcattca agaacacttt aatgatattt tactatgtgg tgttgatact aatgaggcga
     5221 gacgaaagga agttgaaaag gaatttggaa taccaactta taatagtcta acagaggcaa
     5281 aaaatttgtt tgaaataaat tcagtttttg tttgtacatc ccctataacc catgaattta
     5341 ttataaaaga ggctttaaat ttagattgta atatctttac ggaaatcaat ttattaaatg
     5401 aatattatga tgaaataatc gaagtagcta aaaagaaaga tagattactt tatctatctt
     5461 ctacattttt gaagagaaaa gaaattcaat atattaagtc taaagtgacg cataataaaa
     5521 aaatttctta tcaataccat gtaggacagt atttacccga ctggcatcct tgggaagatt
     5581 atcgcaattt ttttgtttca aacaaaaaaa ctaatgggtg tagagaaata ttcgctattg
     5641 aattaccttg gattatagat acatttggga aagttaaatc gtttactgta atgacccaga
     5701 aattatccaa tcttgatatt gattattcgg acagtttcac tcttattatt gagcatgaaa
     5761 ctggtatagt tggaacttta agtgtagatg ttgtttcaag agtagctaaa agaaatttaa
     5821 ctataattgg tgaaaagatt ttaatagaat gggaaggtaa cccggattct ctattttcat
     5881 ggaatgaaga aacttctagt atgacagata ttcaattgta cgatgaggtt atgcatgata
     5941 ataattattc acatactatt attgaagatg catatttaga ggagattaaa gaatttttca
     6001 cgcttttgga aaagaaaatg gaaccccaat atacttttga aaaagataag tatattatta
     6061 acttaattaa cgacattgag gtaaattaat gatgaaagta attttttttg ggttagggtc
     6121 aattggaaag agacatctaa aaaatctaat aagctatgga aaaaagaatt ctattaattt
     6181 tgatataaca gcttatagta gcagtggaaa aaaagagttt gaagaaataa cgtatattca
     6241 aaatagagac gatttggata cacattatga tatagctttt attacaaatc cgacttttaa
     6301 gcatctggaa acactaaatg taattgttga aaaagttgat tacatatttt tagaaaaacc
     6361 tgctttttct gagagtattg atcttactca atataattat gattacaaaa atatttatgt
     6421 tgctgctcca ctgaggtata aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa
     6481 aaaagtatat tctgtaagag ctatttgttc tacttatctt cctgattgga gaaaaggtac
     6541 tgattataga aataattatt ctgcctttaa atctatgggg ggag
//
LOCUS       DARBEG010000114         6364 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000114 DARBEG010000000
VERSION     DARBEG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.117"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..2555
                     /locus_tag="SCK20_002532"
     CDS             <1..2555
                     /locus_tag="SCK20_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG1146549.1"
                     /translation="TADSQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDND
                     IEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHI
                     FLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGK
                     STKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQA
                     DYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHC
                     LHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEK
                     VACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKA
                     RREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQ
                     LTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKS
                     TTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNF
                     SSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEF
                     SDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLP
                     EHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTV
                     FDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQ
                     VKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            2737..2976
                     /locus_tag="SCK20_002533"
     CDS             2737..2976
                     /locus_tag="SCK20_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146550.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2992..4365
                     /locus_tag="SCK20_002534"
     CDS             2992..4365
                     /locus_tag="SCK20_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1146551.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4369..6003
                     /locus_tag="SCK20_002535"
     CDS             4369..6003
                     /locus_tag="SCK20_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1146552.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6166..>6364
                     /locus_tag="SCK20_002536"
     CDS             6166..>6364
                     /locus_tag="SCK20_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146553.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2230 a         1041 c         1287 g         1806 t
ORIGIN      
        1 ctactgcaga ttctcaagca attattaact atttagaaaa acagaaattg tctgaaggta
       61 cagtaaacat aacttataat cctattaaac aagaggtgat tacacaaaaa aagcattctg
      121 tgagagacaa tgatattgaa aaaattcggt ttgtatttat tgatattgac cctaaaagga
      181 aagagggaag tgcaacagat agcgaaaaga aaaaggcaga aaatgttatg gaacaagtag
      241 aacgttattt gaaagaaaag agaatagaat catttgtaaa agttgatagt gggaatggct
      301 accatatttt tcttcccata aatgaacaac caaataatca cgagacgatt cttactattc
      361 agaattttct tcaattactt caccgaagat ttggagtaga agatgaagtt gatattgata
      421 cttctgttta taatccttca agattatgca aattagttgg tacaccagcg gtcaaaggaa
      481 agtccacaaa agagcgtccg catcgtttat ctagtttcct ttctattccc gataacatag
      541 atagaagtaa ttgttttaaa attcttgaac agatagttga agaaaattcc atgatgtcaa
      601 cctctagagt aaaaagccat ccatctttta gagaagtgtt accttttatt caagcagact
      661 ataaacaatg gttagctcac tatcctgaat taacttatac cataaaagag ggcgatcata
      721 aaggagttac cttacttata tttgacgaat gtccattaag aaaacacacg aataattcta
      781 atggttcttg tttgtctgtt acggctgata ataagattcg atttcattgt ctgcatgctt
      841 cggataaaaa taaagatatt caagactttg taaaaaaata tccgttgcca aaacctgtaa
      901 atccttctgt agaatcatta gaatccaatc aagaatatta ttttgaccag tttaaactga
      961 atcaagaagg agtatttttg gtgaaagaag gtcaagaaga aaaagttgct tgtccgatgt
     1021 ttattaagca aatacgcaaa aatatcgaac aaaaagtcat tacacttgtt cttgcctatt
     1081 atgtcgatca ttgttgggat gaaatagaaa tagaaggtgg atgtttacaa actaataatt
     1141 ttaaacaatt agcaaaacat ggcttaatca ttaaagctag aagagaagct gaagtcgttg
     1201 actttttaca gattcaacgg gagaatgctc caatcttttt tgaacatgaa tctcttggct
     1261 ggaaaaaaga aaatgaagca atgatttttc agttacaaga ttcttttgga cgtgaaattc
     1321 cttctgtatt aactaaaaac tcatgctacc aattaacaac taatggtaca gaagcggaat
     1381 ttgacgcatt ggttcataag tatgttttag gaacgaactt agaacttgct tggtcgattg
     1441 gtattgcttc tattgtgtta ggctatttaa acgttacgaa ggctaatact tttttgagtt
     1501 tactaattaa cctacagggc aagagcacaa cgggtaaaac cactacgtta cactttattg
     1561 cctctttatt tggaaatcca actacggttc ttagaaatat gaatgctacc aataaagcca
     1621 ttataaagct agcatcaaac aatcagggag gtgttccact aatactagat gagttaggag
     1681 cagcaactca aaataacttt agctcgttga tctatcaatt agcttcagga gaagaaaggc
     1741 taagattaga ttctaattgt cagcttatgc cacaagagac tttttctgtt ttgaccctta
     1801 cctcttctga agagcgtttg gaaaattatt tcaatgagaa attaactggt ttgaaagttc
     1861 gatatatcga attttctgat atcaagttta caaaggatgc gaatcacgca gagaaagtta
     1921 aaagtctttc ttctaatact tttggttggg gtataaagtt atttcttttg aaactctttg
     1981 aacaagggat tacaaaaatt gaagcaactt ttgtcgaagc tcaaaaaaaa ctatgggctg
     2041 ttttacctga acattctttg aagcaacgca ttggtaataa tctagctttg atttatagtg
     2101 gagccctcct aattcaagat atactgggct atcctttacg attagagcat atacagacgg
     2161 tgcttattaa ttcgtacgaa cagttaattg tagatactag tttaggaagt gagcaaacgg
     2221 tatttgaccg agtgaaaaca ttgttggtga tgaatagcca taaattttta acaaacagtg
     2281 acaacaaaca tacgcccagt acgatttggg gaaaagtatc aattcaaaaa aatggggata
     2341 ttaaggtaaa tatttttcct atcccgtttg aggacttgtt aaggcgagaa tttcaagtaa
     2401 aggatctaac ctatcttttt aggcaattgc ttgattcggg taatatgtgt gcagaaaaag
     2461 ggagaagaac gaaacgtatt cacatgaatc gtcaagttct tccaacatac gagattctat
     2521 taccttcttc actcaaatcg tattttcgat tgtaatttag aataaaccaa agtatgaaag
     2581 tgaggcacca tgttatggaa atatttctta ttgatttagc aaaacaactg attacttatc
     2641 tgtatgatag cgagcaaacc caaacggaag taaatgaagc ttgatctatg cttcatttac
     2701 ttcattcaat caaacatgaa agaaaggaaa attctaatga ctaaaaataa aatgacacta
     2761 aaagcagagg ttcttctgta tattcaagaa catttttcaa atcaagcctt ttttacaaaa
     2821 ccaatttact tggatttcga aataagaggg gtatctgctg gatctatcgg gggtactctt
     2881 caagcgttga agaacgaagg atatttagaa aatcactttg tgcaacgttc gttcaataga
     2941 agagtcgtga aaaaatggta tttagttcat tcatagagag gggaatcaag catgcgtgtg
     3001 gcttgttatg atcgaacgtc tgtttatgaa gaagcgaaaa ttagtaatga cgctcaatat
     3061 gatgcagtga aagagtatgt tttagagaaa tatgaacaaa ttccccaacg attttcagac
     3121 aaaggagaat caggggaaaa agcggagcgt ccagcttttc gacagcttag agcttgggtt
     3181 tgtgcgtcag aagaagaggg ggtgatctat gtttggcgtt atgatcgatt ggcacgtcaa
     3241 gccaaaatag ccttagaatt tattgagttg tgccaacaac atcaggttca agtgtattcg
     3301 atttctgaac ctcttcctgc ttctttctca cacgcgtcaa cgaataaact atttgtgcaa
     3361 ttattgttca cttttgcaga acaacaacga aacgtcatta ttgagaatgt tcgtgccgga
     3421 ctttcctata aaaagcaaca acggcaatat ttgtcttctg cggtaccata tgggtatcgt
     3481 ttagtgaatg ggaaaatcca acaagaagta cacgaagcaa ggattgttcg ctatattttt
     3541 caactttatt tgaccaaaaa atatggctac aaaaagcttt gtcaacgatt gacgcaacaa
     3601 aagcttttct tccgcgaacg tccttttcag ccctatcata tttatagtat cctaaaaaat
     3661 ccattgtact atggcgaaat caaaggagga tctttgggga agtatctagg aacctttgaa
     3721 ccgattctct cgaaagcaac ctttttacaa gcccaagaga ttcgccaatc aaggtgtaca
     3781 gctaaaaaag atacgtatct gtacttatta cgtcaaaaaa ttcggtgtcc cttctgtggt
     3841 cgtcatctga gttcgaagta tcaatggaat acaaagaaga cgaagacgct tcattattat
     3901 cattgtaccg accgaagctg ccaaggaatt tttcttcggg cagaaagggt tgagtcagaa
     3961 gtattggaaa agatacaggc tttttttcaa tacgaaggcg tctatcaagc cttgattgcc
     4021 caattaacgc aacaattagc cgaaaagcga aaagatgaaa aacgcaaagt aatccaacaa
     4081 aagaaacaag aagcacaatg gattaaggca tttgaagagg gcaattgttc actggaagaa
     4141 ctcaagcaac aactgcaagc tcttcaaaag ccgatgtcta cttcttcgtt tctttcactc
     4201 caacagtgtg aagcaaaagt gaaagaatgg cttcaactaa aagacctctc tagtcgacaa
     4261 ttattatgtg actatgtttg tcggattgag gtaacaaagg ataaaacagt ttgtgggatt
     4321 tacttccgac aactgacaca aaatatttta gaaaggaacg aatgatctat ggaaatgata
     4381 caaaccaaac gagtcgcatt gtatattcgc gtgagtacac aagaacaagc tttagaagga
     4441 catagtattc aagcacaaga acaaaatgga cgacagtttg ccgaacggat gggctatgaa
     4501 gtggtagaag tttatgccga tgaaggaatc agtgggaaat ccacaaaaaa tcggttcgcc
     4561 tatcaacgaa tgatgaacga tgctcggaac catcgttttg atctactagt gatttggaaa
     4621 ttgacccgtc tcggacgcaa tatgttagat gtcttacaaa cagtcgagga attattgcga
     4681 tatgacgtag gattacattc gatctcggaa caatttgacg tgaccacttc gacaggtaaa
     4741 ttgatgttac aactccttgg gagtttcggt gaatttgaac gcaaccaaat tgcggagaat
     4801 gttcaaatgt caatgcaaag cttagtgaaa gataaaaagc gttatgcagg aggccgacgt
     4861 ttaggctatg tcagtggtat tgacacgaat ggtcaaaaac aattggtcat tgaaccagaa
     4921 gaggcgaaaa ttgttcgtct gatctatgca aaatatttag gcggagcagg gtatcgagcg
     4981 attgccaatt ttttgaatcg tcaaggatat caaacagtca aaaagaatgc tttttctacc
     5041 acggcagtca aagatatctt acgcaataaa atttatgcag ggtatttaga atatgcgcgc
     5101 tacatcaatt gggaaaccaa acgacggaag gggaagaatc cgagaccgat tttagtgaaa
     5161 ggaacccatg aaccgattat ttcggaagaa gattatcaaa aagtacaaga acgccttgct
     5221 ttagaaggaa aacaacccaa atggaaccat acaggtgaaa atgtattgac aggcttgtta
     5281 cgttgtccag aatgtggcgc accgatggca gcgagtaatg tcacaaatac cctaaaggat
     5341 ggcacaaaga aacggattcg atattattct tgttcagtct ttcggaataa aggagcaagt
     5401 gtgtgtcatg ccaattcgat tcgtgccgaa caagcggaaa catttgtcgc cgaccgatta
     5461 aaggagatcg ttcagctacc agaagttctt ccccgattgg tggcagcgtt aaatgaagaa
     5521 atagtgcgac aaagccaacc attggaacaa gaactcgtgg tgttactgga acgaaaggaa
     5581 gaattgaaga ctaaaattga aaaatgggaa gctgctttgg aagatagtcc tgaattgttt
     5641 ccgatgttaa aagaccgctt agacgaattg actgaaaaac gacgacagtt gcacattcga
     5701 gaaaatgaaa tcttagggat ctttcaacaa ggagagccga ttcaagtcaa agatgtccaa
     5761 cggatattga ctaatttgga tcgttttctt gcccagagtg agaagaagca aatcaaagcc
     5821 ttgtatcgta cctttataga gaaaataacc tttgatccta accataagga gatgttggaa
     5881 attacgatga aattcacgca tgccgtggta caacaattga atgagcaata tcaaatagcc
     5941 gtatccaaaa caaaggatac ggctattttt gtgttaaaaa caccctttac gttaacgatc
     6001 tagtctaggt tatatcaaac aacgttagag gggcgctaac gcttctctag cgatttgaaa
     6061 tcgttagaga agtagttagg gaggtcctta tcataaatag ggatatttat taatgaatat
     6121 ttgaaatata attgtatata agaattaaaa agagtgggga tagatatgaa aaagatgggt
     6181 aagtgtattt tatgtgaaaa atacactgaa cttactaaag aacatgtacc acctaaaaaa
     6241 tcagggaata ctggaggtaa aaagaccaga actggtaatt tagatgattt ccttaagagc
     6301 gattttacaa aaggtgattt tccgaagggg attaaaagga aacctcaagg aaatgtttat
     6361 tata
//
LOCUS       DARBEG010000115         6290 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000115 DARBEG010000000
VERSION     DARBEG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.118"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..658
                     /locus_tag="SCK20_002537"
     CDS             <1..658
                     /locus_tag="SCK20_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146554.1"
                     /translation="ASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLVGVIFALSIVLS
                     GLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTACYEDMNNIFVD
                     QEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQTEEACCNLFSDT
                     IPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLESVKEWLDQQQQN
                     "
     gene            complement(779..865)
                     /locus_tag="SCK20_002538"
     CDS             complement(779..865)
                     /locus_tag="SCK20_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1146555.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            1541..2491
                     /locus_tag="SCK20_002539"
     CDS             1541..2491
                     /locus_tag="SCK20_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG1146556.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            2512..3045
                     /locus_tag="SCK20_002540"
     CDS             2512..3045
                     /locus_tag="SCK20_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1146557.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            3061..3231
                     /locus_tag="SCK20_002541"
     CDS             3061..3231
                     /locus_tag="SCK20_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146558.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            3681..4214
                     /locus_tag="SCK20_002542"
     CDS             3681..4214
                     /locus_tag="SCK20_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146559.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            4226..4429
                     /locus_tag="SCK20_002543"
     CDS             4226..4429
                     /locus_tag="SCK20_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146560.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            4760..5140
                     /locus_tag="SCK20_002544"
     CDS             4760..5140
                     /locus_tag="SCK20_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1146561.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            5122..6036
                     /locus_tag="SCK20_002545"
     CDS             5122..6036
                     /locus_tag="SCK20_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1146562.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            5996..6274
                     /locus_tag="SCK20_002546"
     CDS             5996..6274
                     /locus_tag="SCK20_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146563.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
BASE COUNT         2318 a         1095 c         1176 g         1701 t
ORIGIN      
        1 tgccagtatt ttaggaatta tttggcttag ctataaggct tatcaaacaa gatattcatt
       61 aaaaggctct aaaattcgat ttaagcgatt tttattggtc ggggttatat ttgccttatc
      121 aatcgtttta agtggtttat tcggcgtttt agagggtaat aaacgtgttt caagaagcat
      181 tttattaggg gatatcactc aaaagtatga agcagcatgt gataaaaaga aaaaagagca
      241 agcattagca caaaaaatgg aggaatttac cgcttgttat gaggacatga acaatatttt
      301 tgtggatcaa gagaaacgtt taacagataa aaacatgaaa aaactcactc gtttttatca
      361 aaacttacca gaaaaacagc aaaaagaggt acaagaaaaa tatgagcaag taaaaaaaga
      421 cgtgcaatat tttaaagata cgcagacaga agaagcttgt tgtaacttat ttagcgatac
      481 aatccctttt tcaaccagtg aacaagaacg aaaagaacgg caacaatcgg tcacatacga
      541 acgttatgaa acactgcttc agcaagcaac aaacatacaa aatccaacga aaaaagaaac
      601 ggcattaaat tatctagaaa gtgtcaagga atggttggat cagcaacaac aaaattgaat
      661 ttgattagca atcatgttaa actgaacctg caaatgatca aacattagat acacgaaagc
      721 cccagagagg tcggattctc tggggctttt tttcaggttg atgtcgcctt tattgatttt
      781 acttcttgtt tcggttattc aaccaataac tgaataaaga gacacaaact tctacaagaa
      841 gggggcaaat gaaccgttca aacattagat acacctcctt ccagtcgcaa gactgtcgga
      901 taggcgacaa gttattatac cttacaaaat aaaatctgtg tggtgttttt aagtatccct
      961 tattttcggg ttgttagggc ttgccctgac cgcctgtaag gcgctttaag aacaagataa
     1021 ggttttttac ccaagactgt cttaaaacga cttagacgag ctatcagagg cttcccccac
     1081 ctccgttact gacttgtttt tagcaaagga gcctttaggc gactgcgcag tacaatgtga
     1141 gagcatagcg atcacacttt tttagcgacc gtagggagca aggttttgaa ttggcagttt
     1201 tgaaaaatgc gcgaacctgt tggtagaaaa aagaagtggt ttttaggcgg gaaattttgt
     1261 caccctcttt tataatctct ttttaaacct ttttttaaac ctctttaccg tcggctgtgt
     1321 ccttactcct tcaatggata cagcgtttta taaactacat tttgtctgct tttaaactac
     1381 attttgtctg cttttaaact acattttgtc tgcttttaaa ctacattttg tctgctttta
     1441 aactcttttt ttgcactcac gtatgttata atatgcatta tcacagacaa aacgttgttt
     1501 ataacgtctg tatccattga aggagtaagg aggaatatct ttggcaaatg aaatcgtgaa
     1561 ataccatcat gaattaaata ccattccgtt gcggaaattt acaccagttg aaatgaatct
     1621 atttttctct attgtgtcta gaatgcgcga aaaaggcgat aaaacagtcc gtcttacttt
     1681 tgaccaacta aaagatttaa gtaattataa agcaactgct aataatcgtt tcattaatga
     1741 tttagaaatg acttatgata aattaatgga tttacgtttt ggtaaaagaa gtgctaatgg
     1801 gctcgatata gatagattcg ttatgtttag tgagtttaaa atacgtggaa ctgctaaaga
     1861 tccatatgta gacatacaag tttacgaaaa agctttacct ttactaaaca atttagatga
     1921 gtgggttcgc tattcactcc aacaatttaa cgaattacaa agtagctatg caaagactat
     1981 gtttcgatta ctaaaacaat atcgaactaa aggttttgct tatttttcta aagaggattt
     2041 tcatgaacta ttagatattc caaaaagtta ctggaacaac caagcaaacg ttgataatcg
     2101 tgttataaag cctatacgac aggaattaac cgcaatcttc aaaggattat caatcaaaaa
     2161 gaaatacgga aaagggcgag gaaaacctgt cgttggctat caatttacgt ttaaaccaga
     2221 aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc aaagaagaac aaaataaagc
     2281 attggcttta gctgataaac aaaaagaata cgaagaacct gctacaacaa ttaacgaaaa
     2341 aatcaaacag aagaaacgag aacttgaact cctccaacaa atcaaacaac tagaaaaaga
     2401 gaaagaagct cttgaaaaac aaacagagtc cgcactttcg aaaaaagaga aaaaaagttt
     2461 attatctgat ttagataatt tattccagta gcaacctagg aggcaaccag tatgcaacca
     2521 caagaaacct ttacaataaa ggaattgtca gaacttttta agatgagtag gcaagcaata
     2581 tcgaaacata tccaaaaatt agattcttcc atgatagcta aaaatgagcg tggttataag
     2641 gttgtattgc gttctggagt attgcaactt gctcgcaact taggcaacca aatgttgcta
     2701 gataggttgc acgagtacga acaagacaac gataaaaatg ttaatatacc aagcaaaaac
     2761 gtggttacaa acaagttgct agatcagttg cgtgaagaaa atgaattttt acgagaacaa
     2821 cttgccctca gagagcaaaa taattctcat atacaaaaac tactcgacca gcaacaacaa
     2881 ctcaccctac aagccaacaa acaaatcgaa aaactgcaag aaaaactaca gttgacttat
     2941 acagaagaag ctccagagaa tgactcaacg actctttctg aagcaacaga aacaatagac
     3001 aaacaaattg atactgaaaa aaaatggtgg caactttgga aataactgga gggaacattc
     3061 atgacaagaa aaaatcttct attcggttct attttactag gaattctagg attcagtccg
     3121 atgttattgt cagtgatcaa taaagggaat aaacatggtg aattgaaatt gaatccaaat
     3181 tattacaaca gtcctaacga agaattgttt gtaaaagaaa cagataaata ataaaaaaga
     3241 ctgctctttc gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcaacgcg
     3301 cttatgaagc gctaccaaaa atttatcata ttttattaaa aataacaacc cagtaaaaaa
     3361 tgactggtaa aaattcacaa agaaacggaa agaagcaacc gcagaaaatc aagcttaaaa
     3421 agagcttttg acctagacca atcgaaaatc acaaagtgag cgcctgtatc tattgctaca
     3481 ggcgctactt ggcatagtat acattatgtc aacaagaatt taatataaca tattttccta
     3541 atgcattcta actataccac attatcaaaa atataaatta tctttactaa ataaaatatt
     3601 gacaagccta taccttttgt tttatgattt tcacaaagaa aacgttttct atatattttt
     3661 ctgtatcagg aggaaattct atgaaaaaat ttactttagg ggtagctttg ttaactattg
     3721 gggcagttgt tttaccgtca actactatag tttatgcttc agaaaattcc caagtggtat
     3781 cattaaacga aaacaatgct actgaaaatg tagttattga gtacactgaa gatggtatgg
     3841 tcgtaactaa atatgaggat ggttatagtg cggaagattt tgcaaattct tcagatatca
     3901 tgacaagagc tgcagttgca agatggggca aaaaggaata tacaaatatt gctgtaacca
     3961 ctggagtagc tgctaatgct attaatgctg ccttttgggc aggagggaaa gctagcactt
     4021 ctatgtttgg tatcccaggt tgggcaattg gtgctttgtt aacaggtgca agttggacta
     4081 aaaaaggtgc ttctccaggg aatgcagttg caaaagaatg ggacaaaaat ggtaatggct
     4141 ggattggatt ttattaccaa aggggatatg atgcagctgg aagagttgta gctactagat
     4201 attcaacgtt ataagttaag gagtgatgtt tttgatatct aaatcattaa aatcattaaa
     4261 aacatttact aaaaagaatc cgcaaatagc tactattcgt atttttatat ttgctatagc
     4321 tgttgctata attgttaata tgctaagcgg agggcaatta tacaaatctg gtttattttt
     4381 agtgttaatc ttagttgtct tagacataat aaagtctaaa aaaaaataaa tcgatacatt
     4441 ttagaagaat agggttgttt gaatattaca aagcaacaat ttcaccttcg gtgaaataag
     4501 cgaaacttta caaaaagtgg ctagcaaatg ctagcctttt tttgtattgt ggagcgtgga
     4561 ttttgagggg gattcaaggg ggagcttgcg ctcctttgac aagcggttat ttgcaccaaa
     4621 tacgtaggta tttggctagc aaatatagag cttgccaagc cctttagtga gctttagtga
     4681 gctttactta ttgtaccttt ttaggtacaa tggtattgcg aacgatacgt ccgtaggacg
     4741 tactgaaagg gtgggactta tgagcgaaca aaatcaatat ttggctagca atcattctaa
     4801 aaaatatacc tatcgatccg agccaaaaca aatcagtttt cgagtgagcg aatccgaatt
     4861 tgcaaagctg aagcagtcag ctgaagcttt gcaaatgagt gtgccggctt tcgtgaaagc
     4921 caaggcacaa aacgcgcgtc tggtgacacc gaaagtggcg ccagacattg ctcaggcaat
     4981 cgcgcgcgat ttagccaaag ctggcgggaa tatcaatcaa attgccaagt ggtgcaacac
     5041 acaccaacac gacgttgatc ctggcgacgc ccaacgctta tccgaaaacc taaaaatcat
     5101 gcaaaaggag ctacaaaaga tatggcaaca gttaaagtaa gccgaaccac ttcttgtagc
     5161 cgagcgatca attacgccga accacgagca accgtcaaaa cagggataaa ctgtgacatt
     5221 gattatgcca aaagcgaaat gaaacaaatt cgcatgctgt atggaaaaga cgatcgtgta
     5281 caagcacact tattgatcca gtcttttcga ccaggagaaa ttaccgctga aaaagccaat
     5341 caactagggg cagagtatgc gaaaaaaata gcccctgacc accaaatcgc gatttacaca
     5401 catacagaca aagaccatat tcataaccac attgtcatta actccgtcaa tctggaaact
     5461 gggaacaaat ttcaagccca tggtcaagca tttttaaaca aatgttatga cctaaatgac
     5521 gagatttgtc ttgaacatga cttaacaatc actgaacgag ggaagaaacc cgaaaaacga
     5581 accatgtctg aaatcaaatt aaaagaaaaa aatgaacctg tttggaaaga cgaaattcgg
     5641 tttgcgattg atcaaacaat gaaaaacaaa aaaactcgaa cgtataacca attttgtgat
     5701 tctcttaaaa cattcggcat tcattgcttg aatcgtggga aaaattttac ttacgaattg
     5761 atccacaaaa agaaaaaagt acgatccaac aaactaggaa aggattacga aaaggagacg
     5821 attttacatg agctggacag acgagaacga gcctacaatc aacaacgaac caacgagcta
     5881 cgaagagctc gagctagcat tcgcgaaact gcaaaagaag tacgaagaga acaacgaact
     5941 cttacgactg aacaaccgga aattagaccg actcgaagaa accatgacca acacgttgaa
     6001 agaggagcag actaccaaca agaattatcg ttctaaaacg ttacaagaca tagaaaatga
     6061 aataaagaaa caacgtgctt ttcttgcacc ttataccaaa gaaagaatac aaactacaat
     6121 tgattcacaa gtcaaaaaat tccaccatgc gctattaaac cagcgaaata aagaacagaa
     6181 aaaagtaatt atgcagcgat caatggatat tcttgccccc ttatgttgct taatcatgac
     6241 actgttagcc ttttggctgg tatggggaga ataaatagaa atggcattct
//
LOCUS       DARBEG010000116         6287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000116 DARBEG010000000
VERSION     DARBEG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.119"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SCK20_002547"
     CDS             <1..177
                     /locus_tag="SCK20_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146564.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="SCK20_002548"
     CDS             190..690
                     /locus_tag="SCK20_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1146565.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="SCK20_002549"
     CDS             787..2634
                     /locus_tag="SCK20_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG1146566.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="SCK20_002550"
     CDS             2637..3533
                     /locus_tag="SCK20_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1146567.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="SCK20_002551"
     CDS             3583..3972
                     /locus_tag="SCK20_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1146568.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="SCK20_002552"
     CDS             3959..>6287
                     /locus_tag="SCK20_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1146569.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DARBEG010000117         6233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000117 DARBEG010000000
VERSION     DARBEG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.120"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(148..711)
                     /locus_tag="SCK20_002553"
     CDS             complement(148..711)
                     /locus_tag="SCK20_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1146570.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(726..1769)
                     /locus_tag="SCK20_002554"
     CDS             complement(726..1769)
                     /locus_tag="SCK20_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146571.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(1902..2306)
                     /locus_tag="SCK20_002555"
     CDS             complement(1902..2306)
                     /locus_tag="SCK20_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1146572.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(2539..3174)
                     /locus_tag="SCK20_002556"
     CDS             complement(2539..3174)
                     /locus_tag="SCK20_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1146573.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(3440..4492)
                     /locus_tag="SCK20_002557"
     CDS             complement(3440..4492)
                     /locus_tag="SCK20_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1146574.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(4505..5443)
                     /locus_tag="SCK20_002558"
     CDS             complement(4505..5443)
                     /locus_tag="SCK20_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1146575.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(5894..6208)
                     /locus_tag="SCK20_002559"
     CDS             complement(5894..6208)
                     /locus_tag="SCK20_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146576.1"
                     /translation="MYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENII
                     LRTEYGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSH
                     RQ"
BASE COUNT         1977 a         1303 c          982 g         1971 t
ORIGIN      
        1 agatggtcat tcatgttaga agaatcaaac tattttttta ctgtcgtaat aatattaaaa
       61 cactagaaca aatcaattaa attccataaa atataaataa tagacctagc atcatctggt
      121 ataaaatgga tgctaggtcc gttatattta ctcaaaattg attctacgaa tagatcttct
      181 tatcatttct attttagtag tcatgcgttg ttgttcttct aaaggtggta atggtataag
      241 tagtttagat aaatttgtac catttacatt tggttgccca gttccgtaag aaattttttc
      301 taataacttc caatataatg gcgagtctaa gaagcagtct acgtattctg ataataattt
      361 ctcaactaac ctaattcgaa tcaagtaaga agcaaaaaca gattcctcac ttatttcctt
      421 aaccaagaaa ctttttccca tagttcctcc tgtccttgct atcaaaatgt cattttcttc
      481 caatcttaaa tcaactagtt tagaattact aatatcacag taaggcacac tggaccaatt
      541 tacacgtccc tcttgtatat ctgtgattct caaaaactta acattgcctt gttttttcgc
      601 cgaatctgta tagccatatt gaatcttagt agaaatactt cctaaataaa cccatagtcg
      661 aacatcgcta gatgattttt cttgtcgttc tttatgaaca acagtgatca tcttgagttg
      721 tccgactatt ttttagttgc agcaatcttt ttgggacgct tatttgcatg aagcaacgtt
      781 gctaagtcta gtaataaaac attggctaac acaggattgg ctttctcgcg atacgctgcg
      841 gcgccacttt caaacaatac tgctagacga tcttctacaa caacgatttg ttccaaatac
      901 ggtggaaatt tgattcgttt taggatccgg ctaggagagt cccgctcgga cactaagagt
      961 tccgaggtat aaggaccata agaacgactg gctaagagat aatggccata aaaagccaca
     1021 ccttgaatct tatcagatgt ttttctgctc attgtcacgg ttggttggat tttgtctttg
     1081 cggcgaattg ctgttttcat ttcagctatc gtcggcaata gatagaaagt caactgtcct
     1141 tcacctttta gtgtgaagtt gccagatacc agttgatcat tgttttgagt caaatacgat
     1201 gcttgtggaa tctctgagag atcagttgtc agccaataag cgattggctg tcccgtctct
     1261 tcagatgtat ctgctaagat atcgatcaaa cgtaaagcgg ctactcgtcc agtagcagat
     1321 cctgtgattg tcaaccataa aagttcccgt tttgtatcat atactaagcc ccctacatgt
     1381 ggccgtttag gtaaaatcag cagtttgatc ggctgtcctg tttctctatc cagtacatga
     1441 ataatagaat gatgctgatg gtcatagcag taggcagaaa taaacacaaa atcttgagaa
     1501 aaagccaacc cttgcggcgt catccattga caaacctcat tttcgccggt ttccggcacc
     1561 accattcttg tttctgttaa tccaggaatg ttatattgtt ttgcttcagc cttttcccaa
     1621 gctgcttgaa caataggata ccgttttaac tttttttcaa tatcctgatg tatccgttgc
     1681 tgcttccaat gcttaaactt taggacatag taaacaagaa ggccaatgac cagcaacact
     1741 aaaatcatta ttgctacccg ctgtgtcatt tttttgcctc cttgttctat tcgatcaatc
     1801 gtccatatac cgctattaat taccgtaact acatgtttct tttactagcg tttctttata
     1861 ggatctttca taatctcatt tatagtaaca ttaagaatcc cttattctac tatagtcact
     1921 tccacatcaa tattgcctct tgttgcttta gagtatgggc aaaatgcgtg ggcttcttct
     1981 gcgattttct ctgcttggtc cttctcaagt cctggcaaag aaacttctaa aacgaccccg
     2041 atttggtagc ccccatcttc taattgaaat aaagataccc gtgctttgac ttcagaatca
     2101 gactcaagat tattttcttc cagcatgtgt tgaaccgcgc cgttgaagca agatgcgtat
     2161 ccagccgcaa aaagttgttc cggatttgtc ccttcactat ggattcctgg gcggtcgatc
     2221 ttcatgtgca tacttccgtt tggtgcctca acttcgccat cacggcctcc acgattgatc
     2281 attgctgttt cgtacatttt tttcattgag atcatccttt ctttcgtcgt tctatattaa
     2341 gtgtaacgag tattttttaa tctgacaaag gtaatgcttg cattcggcga catcagttag
     2401 acaacatcga tgatcagtga tagaagcagt ttatctatac aaaaaaagaa gggttctatg
     2461 atatttggtg tccttactcg ccttgagtaa caacaccaaa tatcatagaa ccacttaatc
     2521 agcttcacac tgctttttct actttcgtag ttgaaaaaaa cttaaacaaa tcatcggcaa
     2581 atagttaaag aaaaaactta atcgttgaaa gaagcccaat tgcttaaaga aggcaaactg
     2641 actaatcgac ggaatccgtg ctaatccgta aagacctgca attagtaaac taataactgt
     2701 taaaacaaga taaaaatggc taagcgtacc ttgaccactt tgacgataaa gcagaacaag
     2761 taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctatat tatggatcca
     2821 agtcgataga ttacaagagc tttcattcgt attgatactg aataagccag taaaaataca
     2881 atcacctatc ccatataaac caactgttgc aacaacaagt attgcaagtg tttttgatgt
     2941 tccaacaaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc cagatacaac
     3001 tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca cactaattac
     3061 ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg tcgataaatc
     3121 actcatcaca cctaataata aaaaataaaa accatatttt tgcaaaaaaa tcattttctt
     3181 cccctacttt ttagtgatta ttcgatacat ccaacaatca aattttttct cattttaaca
     3241 catattttta ttctagttgt cttttgaaca atcaataaca taaaggtgag tttcttctac
     3301 attttaaaaa atcgaatggc actgaagagt tcagttgtca gcatcaatct cctactcaaa
     3361 aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac ttacgcatcc attgattgaa
     3421 aatttcttag taaaatctac tagcctaaaa atttgaagac ttcttctcga tcatacagtt
     3481 taaatacttt ctcgcataaa aattgagtaa ttagtgccgc aaaaacaggg atgactaacc
     3541 aacaaagaac aacaagaaag ccatttagcc ctgcatctaa agaagccaac ggaccaacaa
     3601 gtccgaccac gccgaaacca gcagaagcag gcgtacctga aatagaaaat agagcgacag
     3661 gaatagctgt aatcactgca ctaactaaac aaggaataag aatcacaggt tttctaaata
     3721 gattaggcat catcatcttc atagcgccta aagcaatcgc aattgtcaca cctgacttat
     3781 taaccttcca agagttcact actaaaacaa aagtggttgc agctacgccc atcgcagccg
     3841 caccagcaga aacaccatct agttggattg ccattccgat tgctactgtt gaaataggag
     3901 agataatcaa aaaggcaaag gaacaaccaa tcaagataga cattaacagc ggttgtaagg
     3961 tagtgaagtt attaatcaat gatccaattg cagaagtaat tgctgttaca aaagggaata
     4021 aataaaaacc aagtaaacct gcacctacac ctacaacaat cggtgttaac acaatcgaca
     4081 ctgacccaaa actatttcca atcagtaaaa tcagcccgac agcaatcgat gctgtcagca
     4141 tcgtattaat aatatcgcct gtaccagcac caacgtaaac acccgtttca cccaaatcag
     4201 gtgtgaattt gactacacca gaaccaacaa aagaagctcc ggctacaacc atcattctca
     4261 tcggatcaaa tccaaattgt aaggcaatca aaccaccaat gatcaaaggt gttgctagtt
     4321 gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg atacgcacta aaatatttta
     4381 aaatggcacc taatactgca ttaggaatca atccaataat gattcctgtt gctgtaccag
     4441 cgaggacttt attcagaaaa attttcggtg atattttttg tgaagtttcc attcaatctc
     4501 tcctttattt tacgttcaaa ctatcttctt ttgcatggac aagctcagtc aagaaatcac
     4561 aataaggcgt cgcaacacca tatttcttcc cttttctaga aattgcgcca ttgatataat
     4621 cgatttctgt taatcgatgg ttcttaatca agtcttgata cattgaagga taatgtaacc
     4681 caattgtttc cgggtcaaaa caagattcac aatgtgcaat gacttctggg acatctagtt
     4741 caatcttctc tactgctgct actttggcaa actcattgac aatcgtcgcc accattttat
     4801 gagcagtcga tgtttttcct agctcagcca tattaacgtc taagattgtg catagtccgt
     4861 tcattgttcc gttcacacat gctttgcgat aaatagaata gtgaatattg tcagaaaaat
     4921 gagcatttaa tcctgattca gacaatttgt ctgctaattt ttttgcagct gcttcttttc
     4981 catcacctaa attttgtaat tctactgaac cacttccaaa taatttgacc tgaccaggac
     5041 cttcaagacc tgccgtccac atggtatttc caatatagat attttccatt ggtacaaatt
     5101 tctcaataat atcttcatgc ccgataccat ttaatagaca taagacctct gtatcttttt
     5161 tgattaatga ttggatatcc tgcagcattt tttccagctg catcgctttg gtaaatagaa
     5221 taatcagatc aacttgatct tctttttcta cttcggattg aagaacgatt ggtagttttg
     5281 cttctacctc tttaccatta aaatttgctt gtaatccatg ttctttgatt tgttggacat
     5341 gttctgccca gccatcgatt aataatactt cgttcccact ttggtgaagc atcaacccaa
     5401 agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt cattgattct tcctcctttt
     5461 tctttctatt atactcattt gagaatcttt tcacaacctc gttttgttat tcttctctta
     5521 tttatcatgt ttatatccac cattcaagtt attttatcag caaatcaata aaaactggaa
     5581 tagaatccca atttcatcgt aagagaaact agactagcaa taagaaaata gaaacattca
     5641 tagaaaaaat ctcactcctc tgattataaa agagggggca ttaacctcgt gtatactaaa
     5701 accttccatt cattcgatgg atgattttct ttttgtgaaa aaatattcat tgtagtacga
     5761 tgttgcgcaa cacagacgga tggtattgct tattatttat taaaatcgaa caatacactt
     5821 atttaaaaac ttctttccca gattttcttc catttttaca gttataacta gtattatcat
     5881 gaaaaatagc cttttattgt ctatgggaat aataatttaa aattggtact tttttattaa
     5941 ttagttcatc gcccaataat ctctccagtt tcttagcatc tcctgactca accggaatta
     6001 tccaagccca gtctaaataa ttttttaaaa tctcatcata ttttgcaaat atttcattgc
     6061 catattccgt ccttaaaatt atattttcag tgccagacaa gtgatttata ttgagttgat
     6121 aaaaaagatc tccatctaaa aaaatatcat tagaagaaga tttccccaca gtaatgacca
     6181 ttccatcagc tctatgtgtt acatacaaat aattgtatga cctcatttca cct
//
LOCUS       DARBEG010000118         6147 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000118 DARBEG010000000
VERSION     DARBEG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.121"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            102..2543
                     /locus_tag="SCK20_002560"
     CDS             102..2543
                     /locus_tag="SCK20_002560"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG1146577.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="SCK20_002561"
     CDS             complement(2873..3577)
                     /locus_tag="SCK20_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146578.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="SCK20_002562"
     CDS             complement(3764..4009)
                     /locus_tag="SCK20_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146579.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="SCK20_002563"
     CDS             complement(4026..4877)
                     /locus_tag="SCK20_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1146580.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="SCK20_002564"
     CDS             5043..6047
                     /locus_tag="SCK20_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG1146581.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1970 a         1064 c         1301 g         1812 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtg
//
LOCUS       DARBEG010000119         6001 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000119 DARBEG010000000
VERSION     DARBEG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.122"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCK20_002565"
     CDS             <1..395
                     /locus_tag="SCK20_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146582.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SCK20_002566"
     CDS             398..1228
                     /locus_tag="SCK20_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG1146583.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SCK20_002567"
     CDS             1244..2095
                     /locus_tag="SCK20_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1146584.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SCK20_002568"
     CDS             2092..2451
                     /locus_tag="SCK20_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG1146585.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SCK20_002569"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SCK20_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SCK20_002570"
     CDS             2624..2929
                     /locus_tag="SCK20_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146586.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SCK20_002571"
     CDS             2929..3240
                     /locus_tag="SCK20_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG1146587.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SCK20_002572"
     CDS             3237..3410
                     /locus_tag="SCK20_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146588.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SCK20_002573"
     CDS             3407..3823
                     /locus_tag="SCK20_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1146589.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SCK20_002574"
     CDS             3823..4491
                     /locus_tag="SCK20_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1146590.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SCK20_002575"
     CDS             4488..4865
                     /locus_tag="SCK20_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146591.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SCK20_002576"
     CDS             4862..5161
                     /locus_tag="SCK20_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146592.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SCK20_002577"
     CDS             5151..5309
                     /locus_tag="SCK20_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146593.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SCK20_002578"
     CDS             5403..5819
                     /locus_tag="SCK20_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1146594.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DARBEG010000120         5832 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000120 DARBEG010000000
VERSION     DARBEG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.124"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(521..1024)
                     /locus_tag="SCK20_002579"
     CDS             complement(521..1024)
                     /locus_tag="SCK20_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1146595.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1383..2408
                     /locus_tag="SCK20_002580"
     CDS             1383..2408
                     /locus_tag="SCK20_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1146596.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2731..3609
                     /locus_tag="SCK20_002581"
     CDS             2731..3609
                     /locus_tag="SCK20_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG1146597.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4085..4201
                     /locus_tag="SCK20_002582"
     CDS             4085..4201
                     /locus_tag="SCK20_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1146598.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5129..5467
                     /locus_tag="SCK20_002583"
     CDS             5129..5467
                     /locus_tag="SCK20_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146599.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5561..5791
                     /locus_tag="SCK20_002584"
     CDS             5561..5791
                     /locus_tag="SCK20_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146600.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1984 a          880 c         1072 g         1896 t
ORIGIN      
        1 acatatctca ttttacgctt ttgttatttt atacttaaaa aataacgaca cagaaaatac
       61 cgtgtctcag atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt
      121 caaaaagtag gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag
      181 gagaaaaacc atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat
      241 aatatacctg agtagggtat aaattaagac ggaattcctt tctagattga gatattgact
      301 aggaagaatg ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag
      361 gggaatatag cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt
      421 agagatgaaa atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac
      481 attggaatgt gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc
      541 gggtcaaaaa taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg
      601 ctagacggtc taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac
      661 ctatcagtcc tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga
      721 ataggaattg aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag
      781 aaaaagaaag tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca
      841 agttataagt ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga
      901 cgagattgac attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga
      961 ataaaatcaa ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc
     1021 gcatagacat ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa
     1081 aagtatgaag tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt
     1141 gggaatccgg tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt
     1201 ttttctgtca aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac
     1261 tttttgatac taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca
     1321 aacgggattg gcatttcatc ttggttcagc tttttttcca attactagga aggagggata
     1381 gattgttcaa gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga
     1441 atctgatgct acttatcttt attgtattat ggttaggtta cataaatcat gattttttga
     1501 tttcaagttt ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga
     1561 aatatttgct gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg
     1621 ggatcttatc gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt
     1681 ccagcagatt cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga
     1741 tgctttctgc catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac
     1801 tttatttttt tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg
     1861 attcacggcc tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc
     1921 ctttagtgat cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca
     1981 gtcacggctt gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc
     2041 tagcagaaaa cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg
     2101 gtgggttatg tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt
     2161 ttctgatcac ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg
     2221 ctaatatttt tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact
     2281 tgatttcttt gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag
     2341 gtgttgcgtg ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat
     2401 tagaatagaa ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc
     2461 tctaattcaa ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg
     2521 tggaaaggaa ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg
     2581 gtaactgaat aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc
     2641 tttcttcata gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat
     2701 atggtgtccg gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat
     2761 ttgatgaaga aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa
     2821 gtcgttatag agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa
     2881 tatagtcatg aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa
     2941 gtctatgaag aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt
     3001 tggaaaaaaa tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat
     3061 gcttttacta gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt
     3121 ggtttttgga tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac
     3181 gaaatgacat attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt
     3241 tctcttcata ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa
     3301 agtgccattc gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca
     3361 gatacttggt tactatcacc caatcttact aaatggttga aaaaagagtc aaaaatctgt
     3421 ctttttgcag ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct
     3481 tggatctttg gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag
     3541 cgacaagcaa aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt
     3601 acttcataag tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca
     3661 cttttgtctc acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa
     3721 taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg
     3781 agttaaacgc ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca
     3841 ataggtaaac tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag
     3901 gtagctaaaa cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg
     3961 atcgactgtt ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat
     4021 cgtaattgtt tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt
     4081 ccatatggaa aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa
     4141 aacacgaaaa cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta
     4201 agaagaagga aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa
     4261 tcggtttttg tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga
     4321 taaattggta gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt
     4381 aactacagaa ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat
     4441 tccctttcaa cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg
     4501 ttcgtttttg aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg
     4561 cgaaatattt catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta
     4621 tcgtttaata agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc
     4681 ttttcttcaa aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta
     4741 aaaagtaacg ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa
     4801 catcaaggaa taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt
     4861 ttagatacag taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc
     4921 tttaataaaa ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt
     4981 cagtcgattg tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga
     5041 agataaaaaa tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt
     5101 ccctctgttt agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga
     5161 taaccttttt cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac
     5221 tccattagct agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca
     5281 gtacgaagtt atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct
     5341 aacaccgatt gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga
     5401 gactgagatc attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg
     5461 gatgtagaca ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta
     5521 gcacaagaaa tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa
     5581 agaaaatatt tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga
     5641 attcaagagt cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc
     5701 gatagaaaaa actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga
     5761 gtttttggat tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca
     5821 catattatgt ta
//
LOCUS       DARBEG010000121         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000121 DARBEG010000000
VERSION     DARBEG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.125"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SCK20_002585"
     CDS             450..1604
                     /locus_tag="SCK20_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG1146601.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SCK20_002586"
     CDS             1598..2020
                     /locus_tag="SCK20_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146602.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SCK20_002587"
     CDS             3143..4075
                     /locus_tag="SCK20_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1146603.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SCK20_002588"
     CDS             4092..4607
                     /locus_tag="SCK20_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1146604.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SCK20_002589"
     CDS             4903..5184
                     /locus_tag="SCK20_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146605.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SCK20_002590"
     CDS             5186..5602
                     /locus_tag="SCK20_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146606.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARBEG010000122         5791 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000122 DARBEG010000000
VERSION     DARBEG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.126"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SCK20_002591"
     CDS             complement(99..1229)
                     /locus_tag="SCK20_002591"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG1146607.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SCK20_002592"
     CDS             complement(1877..3079)
                     /locus_tag="SCK20_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1146608.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SCK20_002593"
     CDS             complement(3139..3342)
                     /locus_tag="SCK20_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG1146609.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SCK20_002594"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SCK20_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SCK20_002595"
     CDS             4879..5691
                     /locus_tag="SCK20_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146610.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          922 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta g
//
LOCUS       DARBEG010000123         5755 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000123 DARBEG010000000
VERSION     DARBEG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.127"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            487..1044
                     /locus_tag="SCK20_002596"
                     /pseudo
     CDS             487..1044
                     /locus_tag="SCK20_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1102..3141
                     /locus_tag="SCK20_002597"
     CDS             1102..3141
                     /locus_tag="SCK20_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG1146611.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3250..4401)
                     /locus_tag="SCK20_002598"
     CDS             complement(3250..4401)
                     /locus_tag="SCK20_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1146612.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4555..5085)
                     /locus_tag="SCK20_002599"
     CDS             complement(4555..5085)
                     /locus_tag="SCK20_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1146613.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5184..5534)
                     /locus_tag="SCK20_002600"
     CDS             complement(5184..5534)
                     /locus_tag="SCK20_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG1146614.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2076 a         1054 c         1073 g         1552 t
ORIGIN      
        1 aattatcaaa gaatcctggt gcaactgaaa gaatttgcaa cgggattctt tcaacttatt
       61 tgttattttt ttctaaaaaa tattaaagat tgttaaattt cactatttca tttcaaattc
      121 cattgcctaa tcattaggaa aagtctatca tgaactcgta aatagaatat agtaataacc
      181 ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca
      241 atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt
      301 acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga
      361 aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg
      421 ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt
      481 ggtggaggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
      541 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
      601 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
      661 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
      721 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
      781 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
      841 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
      901 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
      961 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
     1021 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
     1081 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
     1141 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     1201 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     1261 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     1321 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     1381 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     1441 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     1501 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     1561 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     1621 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     1681 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     1741 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     1801 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     1861 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     1921 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     1981 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     2041 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     2101 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     2161 atagcttttg acagtctagg aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc
     2221 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     2281 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     2341 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     2401 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     2461 gacagttctt ggggatcttt taaaacaacg cgtgtcagta gtgatgtatc acaagtagac
     2521 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     2581 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     2641 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     2701 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     2761 atgtattctg tttttaccaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     2821 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     2881 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     2941 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     3001 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     3061 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     3121 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     3181 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     3241 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     3301 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     3361 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     3421 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     3481 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     3541 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     3601 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     3661 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     3721 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     3781 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     3841 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     3901 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     3961 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     4021 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     4081 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     4141 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     4201 taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     4261 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     4321 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     4381 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     4441 cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa
     4501 agattttttt ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg
     4561 tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa
     4621 aataaagccg aatatttgga atagaagcat caaacttttg aatggattga acaataagac
     4681 gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt
     4741 catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg
     4801 caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa
     4861 aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc
     4921 aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg
     4981 aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct
     5041 taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct
     5101 ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa
     5161 ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt
     5221 gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg
     5281 tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca
     5341 ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct
     5401 gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct
     5461 tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata
     5521 cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat
     5581 ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaggttctat aatattttgt
     5641 gttggtggaa aatcgaaggc gattttctac taacactttt tcttcgtttc aactcaaaaa
     5701 gagactcact tttggtaaaa tttagtcgac caaaacgtca tcctaccaaa ggagt
//
LOCUS       DARBEG010000124         5559 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000124 DARBEG010000000
VERSION     DARBEG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.129"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..2009)
                     /locus_tag="SCK20_002601"
     CDS             complement(60..2009)
                     /locus_tag="SCK20_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1146615.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="SCK20_002602"
     CDS             complement(2051..3109)
                     /locus_tag="SCK20_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG1146616.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="SCK20_002603"
     CDS             complement(3228..3683)
                     /locus_tag="SCK20_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146617.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="SCK20_002604"
     CDS             complement(4267..5070)
                     /locus_tag="SCK20_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1146618.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1631 a         1101 c          768 g         2059 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatatttaat
     5461 aaaggaggat tactcctctc ttatttcaaa atcttaaaaa taaaaaatct taaaaaaaac
     5521 taacactatc taggagttta ttttgcttgt gtaactttg
//
LOCUS       DARBEG010000125         5534 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000125 DARBEG010000000
VERSION     DARBEG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.130"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..988)
                     /locus_tag="SCK20_002605"
     CDS             complement(<1..988)
                     /locus_tag="SCK20_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG1146619.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGN"
     gene            1140..1835
                     /gene="vanR"
                     /locus_tag="SCK20_002606"
     CDS             1140..1835
                     /gene="vanR"
                     /locus_tag="SCK20_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1146620.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1813..2967
                     /locus_tag="SCK20_002607"
     CDS             1813..2967
                     /locus_tag="SCK20_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1146621.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3182..4150
                     /locus_tag="SCK20_002608"
     CDS             3182..4150
                     /locus_tag="SCK20_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1146622.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4143..5174
                     /gene="vanA"
                     /locus_tag="SCK20_002609"
     CDS             4143..5174
                     /gene="vanA"
                     /locus_tag="SCK20_002609"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1146623.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5180..>5534
                     /locus_tag="SCK20_002610"
     CDS             5180..>5534
                     /locus_tag="SCK20_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG1146624.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSR"
BASE COUNT         1670 a         1078 c         1251 g         1535 t
ORIGIN      
        1 tatttccgat aaccgtgtat tgaaaggcat cttcgaagcc attatccaat atttcgcagg
       61 cttttgtata tttaggctgg tcgatatatt caccgactaa ggcattctta gctgttcgag
      121 cgagttcaat atccgtaaac ttaaagatcg cttttactgc ttctctaaac ggttttgaat
      181 tcttttttgg aatggaactg aagatgtttc ttaaaaaatg gacctggcat ctctgccaac
      241 ttgcgttggt aaatgactta cgaatcgcag acactaggcc tttatgggca tcagaaatga
      301 tgagttctgt cccctttagg ccgcgttctt ttaagtattc aaagaagatg gaccatgtgt
      361 catcactttc ttcattttga atcatgaagc caatgatttc acggtcgcca ccttctgtta
      421 tcccgatcgc aatatggcag cttttagaaa gcactcgatg gtcctcacgg acttttatgt
      481 agagaacatc agtcatcaga taaggataat tcgtacctga gagtgaacgg ttctgccatt
      541 cgttgaccat cgggtctaac tgctcagtca ggctagaaac aaaagatttc gatacagatt
      601 ttccacatag ctcttcaaca atctttgaaa ctttacgtgt cgaaacgccc gaaacataca
      661 tctcaagcat tgaagcgagc agtgcctttt catttcgctg ataacgctca aacaccgtcg
      721 gtgaaaattc accatcacgt gttctaggca cttttaattc gagtgtaccc acacgagtcg
      781 taaagtctcg ctcataatag ccatttcttt gactctgacg actttctgaa cgttcatagt
      841 catcggcttg aatatattct gttcgttgat tttccatcaa ttggttgaaa acagtggtta
      901 aaatattttt agaaacatca tcttttaccg aatgttcaat aatactttga acctcttcgt
      961 tgttcagtgt aaaatgtact tgggtcatgt aacgtcctcc tgggtatgtt tttgtggtta
     1021 aaaacattgt accgtaaaag ggctgttaca tggcctttta tcttttacac aattatatgg
     1081 actttatcct ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta
     1141 tgagcgataa aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat
     1201 acttaaaaaa cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat
     1261 gtatagacaa gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg
     1321 gccttactat ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg
     1381 ggaaagatac agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa
     1441 cgaagccctt tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca
     1501 aaaaattcag tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca
     1561 ttaatgttaa cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg
     1621 agttttcaat actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc
     1681 tatttcatga gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc
     1741 atatccggca tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa
     1801 cggtatgggg ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa
     1861 cgaaaacttt acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat
     1921 attcgttcaa tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa
     1981 tatgacttaa atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata
     2041 gatatcttta tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt
     2101 tcaaaattcg caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac
     2161 gaagataaac aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca
     2221 ttaaaacgga ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat
     2281 gacgttgtta tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat
     2341 ttgagcctgc ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat
     2401 atcacgttgg acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca
     2461 cggtataacc tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg
     2521 gtgcagatga ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt
     2581 cacgcccccg aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac
     2641 aacattttga aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg
     2701 ggcctctccg gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat
     2761 aagctagctg ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg
     2821 ggtggcgcgg gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag
     2881 atttacgcgg aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca
     2941 gacttggttg ataaaaggag gtcctaagag atgtatataa ttttttagga aaatctcaag
     3001 gttatcttta ctttttctta ggaaattaac aatttaatat taagaaacgg ctcgttctta
     3061 cacggtagac ttaataccgt aagaacgagc cgttttcgtt cttcagagaa agatttgaca
     3121 agattaccat tggcatcccc gttttatttg gtgcctttca cagaaagggt tggtcttaat
     3181 tatgaataac atcggcatta ctgtttatgg atgtgagcag gatgaggcag atgcattcca
     3241 tgctctttcg cctcgctttg gcgttatggc aacgataatt aacgccaacg tgtcggaatc
     3301 caacgccaaa tccgcgcctt tcaatcaatg tatcagtgtg ggacataaat cagagatttc
     3361 cgcctctatt cttcttgcgc tgaagagagc cggtgtgaaa tatatttcta cccgaagcat
     3421 cggctgcaat catatagata caactgctgc taagagaatg ggcatcactg tcgacaatgt
     3481 ggcgtactcg ccggatagcg ttgccgatta tactatgatg ctaattctta tggcagtacg
     3541 caacgtaaaa tcgattgtgc gctctgtgga aaaacatgat ttcaggttgg acagcgaccg
     3601 tggcaaggta ctcagcgaca tgacagttgg tgtggtggga acgggccaga taggcaaagc
     3661 ggttattgag cggctgcgag gatttggatg taaagtgttg gcttatagtc gcagccgaag
     3721 tatagaggta aactatgtac cgtttgatga gttgctgcaa aatagcgata tcgttacgct
     3781 tcatgtgccg ctcaatacgg atacgcacta tattatcagc cacgaacaaa tacagagaat
     3841 gaagcaagga gcatttctta tcaatactgg gcgcggtcca cttgtagata cctatgagtt
     3901 ggttaaagca ttagaaaacg ggaaactggg cggtgccgca ttggatgtat tggaaggaga
     3961 ggaagagttt ttctactctg attgcaccca aaaaccaatt gataatcaat ttttacttaa
     4021 acttcaaaga atgcctaacg tgataatcac accgcatacg gcctattata ccgagcaagc
     4081 gttgcgtgat accgttgaaa aaaccattaa aaactgtttg gattttgaaa ggagacagga
     4141 gcatgaatag aataaaagtt gcaatactgt ttgggggttg ctcagaggag catgacgtat
     4201 cggtaaaatc tgcaatagag atagccgcta acattaataa agaaaaatac gagccgttat
     4261 acattggaat tacgaaatct ggtgtatgga aaatgtgcga aaaaccttgc gcggaatggg
     4321 aaaacgacaa ttgctattca gctgtactct cgccggataa aaaaatgcac ggattacttg
     4381 ttaaaaagaa ccatgaatat gaaatcaacc atgttgatgt agcattttca gctttgcatg
     4441 gcaagtcagg tgaagatgga tccatacaag gtctgtttga attgtccggt atcccttttg
     4501 taggctgcga tattcaaagc tcagcaattt gtatggacaa atcgttgaca tacatcgttg
     4561 cgaaaaatgc tgggatagct actcccgcct tttgggttat taataaagat gataggccgg
     4621 tggcagctac gtttacctat cctgtttttg ttaagccggc gcgttcaggc tcatccttcg
     4681 gtgtgaaaaa agtcaatagc gcggacgaat tggactacgc aattgaatcg gcaagacaat
     4741 atgacagcaa aatcttaatt gagcaggctg tttcgggctg tgaggtcggt tgtgcggtat
     4801 tgggaaacag tgccgcgtta gttgttggcg aggtggacca aatcaggctg cagtacggaa
     4861 tctttcgtat tcatcaggaa gtcgagccgg aaaaaggctc tgaaaacgca gttataaccg
     4921 ttcccgcaga cctttcagca gaggagcgag gacggataca ggaaacggca aaaaaaatat
     4981 ataaagcgct cggctgtaga ggtctagccc gtgtggatat gtttttacaa gataacggcc
     5041 gcattgtact gaacgaagtc aatactctgc ccggtttcac gtcatacagt cgttatcccc
     5101 gtatgatggc cgctgcaggt attgcacttc ccgaactgat tgaccgcttg atcgtattag
     5161 cgttaaaggg gtgataagca tggaaatagg atttactttt ttagatgaaa tagtacacgg
     5221 tgttcgttgg gacgctaaat atgccacttg ggataatttc accggaaaac cggttgacgg
     5281 ttatgaagta aatcgcattg tagggacata cgagttggct gaatcgcttt tgaaggcaaa
     5341 agaactggct gctacccaag ggtacggatt gcttctatgg gacggttacc gtcctaagcg
     5401 tgctgtaaac tgttttatgc aatgggctgc acagccggaa aataacctga caaaggaaag
     5461 ttattatccc aatattgacc gaactgagat gatttcaaaa ggatacgtgg cttcaaaatc
     5521 aagccatagc cgcg
//
LOCUS       DARBEG010000126         5220 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000126 DARBEG010000000
VERSION     DARBEG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.131"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SCK20_002611"
     CDS             complement(<1..1256)
                     /locus_tag="SCK20_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1146625.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SCK20_002612"
     CDS             complement(1270..2487)
                     /locus_tag="SCK20_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1146626.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..4072)
                     /locus_tag="SCK20_002613"
     CDS             complement(2477..4072)
                     /locus_tag="SCK20_002613"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1146627.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            4197..4982
                     /locus_tag="SCK20_002614"
     CDS             4197..4982
                     /locus_tag="SCK20_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG1146628.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1574 a         1051 c          862 g         1733 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatcg acatagcgtg ggatatttaa gttatagtca ttttctttga
     2701 tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca gcgtaggttt
     2761 cgaggatttt ttgaatgttt tcttccgata atttattctg atttttccct ttaacaaatt
     2821 cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc aatacaatca
     2881 ccgtcgttgg aatcgatgta ccaaagaaca agttcgcagg cataccgatc acagcataaa
     2941 tactgccgtc ttctaataat ttttgacgaa tgaccccttc tgcagcacca cggaataaaa
     3001 ccccgtgtgg caagacgata gccatagtcc ctgtttcctt caaatgatag aaaccatgaa
     3061 gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccgtagcga ttaaaccgag
     3121 aatcatctaa gaaggtcgta tctgctgacc aattagccga ataaggtgga ttcatcacta
     3181 ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta tccccattgc
     3241 ggagattcat ttcttcggca tctaccccat gtaaaatcaa gttcattttt gctagattgt
     3301 atgtcgttgt atttagttct tgcccatgat atttaacgtt atctggatga gttaagtagt
     3361 tacgtacatt taacatcaag gaacctgaac ccatggttgg gtcaaagaca ctaaagaatc
     3421 gtcgttcttt ttgatccaac gtcacgattt gtgccatcat atcagaaacc atatgaggcg
     3481 tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc agaaattcat
     3541 acgcatcccc gatcacatcc ccatcgtgac ctaaaacttc cacgtcattc aatttcttga
     3601 taacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt tttgattgga
     3661 gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat tgcacaaagg
     3721 ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct aagacgttga
     3781 aaagatactc tggtgagatc gaatacccca aaatatcaac gattgtggca atcaggtctt
     3841 cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg cttactgtgt
     3901 catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaaa cggtcggata
     3961 aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca tccattttac
     4021 tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc aaaaagatac
     4081 actcctatct cttatttaaa gttgctatat accctcttat tttagcatac tcccttttag
     4141 aaaaagcaaa aaaaaaagaa aaaatcttat tcttgataac aagctacgtg acgtatatgc
     4201 ctaacaaaaa tagaactttt gaatcaatct taaatgaagg tttaaccaca gaaccggcat
     4261 ccattatttt tgatgaagtt atcgatgacg ttgaagatag taagtatcat aaaaatttaa
     4321 aagaactttt ttattttgca ctcaacaatg gaccaagttt gttcactggt atatcagtac
     4381 ctgatagtga taatccatta attatggcaa aaatttactt aacaaaatgg tgtaataaat
     4441 acatcaggga taggaataac ccagcattaa aaaaacctct taaaacattt ggcgaaaaag
     4501 atgctgcttt aactactaga gtagcagcaa atgctaatat tgatgatcaa aaagtattga
     4561 atgactactt gcgtggacat tttttatata tgagcgctga gaacatgaac ggaagtattt
     4621 tagaggaata tttagccgaa gtgttagaac ctgaaggttg gatttggtgc gccggctctg
     4681 tatatcgagc tgttgatttc tgttatttag gaacctcacc aattctttta caagtgaaaa
     4741 ataaatacaa tacagaaagc agttcgagtt cagctataag agtaggaaca acaattagaa
     4801 aatggaatcg tttaaataag tcgacaaaaa tttctggttt ggatagccct atccccaatt
     4861 ggaaagcatt aatagaaatg actgaagcta gtaaggaact ggcagctaag ttaactgaaa
     4921 atagttattt agcttatatt aatgaaaaat ccacacgaga attatggaca ctcgatgact
     4981 aacaatatcc ttattagtat ctatccacga tacatggaaa aaattataaa caaacaaaaa
     5041 aactttgaat ttagaaattt ctatcttacc tcagatcctt ataatatctt ttggatttac
     5101 gaaacaatcc ccaccaaagc tttaaagtat aaattgattg taaaaaatcc aattactaaa
     5161 cttaagaaaa atcaacacta ctttttagga gacgatgagt ttgaacaatt agtttaaaag
//
LOCUS       DARBEG010000127         5152 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000127 DARBEG010000000
VERSION     DARBEG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.132"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SCK20_002615"
     CDS             8..649
                     /locus_tag="SCK20_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146629.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SCK20_002616"
     CDS             708..1148
                     /locus_tag="SCK20_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146630.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SCK20_002617"
     CDS             1160..1828
                     /locus_tag="SCK20_002617"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1146631.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SCK20_002618"
     CDS             1892..2515
                     /locus_tag="SCK20_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG1146632.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SCK20_002619"
     CDS             2571..2957
                     /locus_tag="SCK20_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG1146633.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SCK20_002620"
     CDS             3170..4561
                     /locus_tag="SCK20_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG1146634.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1707 a          861 c         1084 g         1500 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac ag
//
LOCUS       DARBEG010000128         4802 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000128 DARBEG010000000
VERSION     DARBEG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.133"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="SCK20_002621"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="SCK20_002621"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG1146635.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="SCK20_002622"
     CDS             1085..2005
                     /locus_tag="SCK20_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1146636.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="SCK20_002623"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="SCK20_002623"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG1146637.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="SCK20_002624"
     CDS             3311..4789
                     /locus_tag="SCK20_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146638.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1689 a          772 c          930 g         1411 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 gg
//
LOCUS       DARBEG010000129         4791 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000129 DARBEG010000000
VERSION     DARBEG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.134"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            60..776
                     /locus_tag="SCK20_002625"
     CDS             60..776
                     /locus_tag="SCK20_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1146639.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            795..2378
                     /locus_tag="SCK20_002626"
     CDS             795..2378
                     /locus_tag="SCK20_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG1146640.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2513..3091
                     /locus_tag="SCK20_002627"
     CDS             2513..3091
                     /locus_tag="SCK20_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146641.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            3037..3555
                     /locus_tag="SCK20_002628"
     CDS             3037..3555
                     /locus_tag="SCK20_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146642.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3563..4603
                     /locus_tag="SCK20_002629"
     CDS             3563..4603
                     /locus_tag="SCK20_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1146643.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT         1771 a          532 c          947 g         1541 t
ORIGIN      
        1 aagagtcgag agcacaaacg gtagacgatg ttatttttat aaaaagatat aatttattaa
       61 tggtcgataa ttatgaaagg aaaaatattc ttaaaaaaaa gagtatagga tgtcgtatga
      121 aaatagtcgc tatagtacca atgaagttaa ataacaaaag attgccacaa aaaaacacga
      181 aagcttttac caatggaaaa ccattgtgtt attatatact ttcaacactt ttaaaagtaa
      241 aacagattga taaagtgtat gtctattgta gtaacccaga tattaaagaa tttataccag
      301 aaggtgtaac atttttaaag agatctagtt ccttagatca ggatacaacg agtattacac
      361 aggtattagc cggtttcaca aaagaagtag aggctgatgt atatgtaatg acacacacaa
      421 cagcaccatt tattagcgaa aaatctattg aaaaaggact agatgccgtg ctgacaggag
      481 aatatgattc tgcatttgct gcaaaaaaat tgcaagattt cttatggaaa gacggtgaac
      541 catttaatta tgaattggac aatattccta gaacacagga tttgccgcct ttatacgaag
      601 aaactagtgg attttatatt tataatcgca atgtgatgac cgagttgcat agaagaattg
      661 ggaaaaaacc ttgcatagtt gaagtaggtg agattgagtc agttgatatt gatgaagccg
      721 aggattttat gattgctgat gcgatttata atcatcttat tttacaaggt agttaagaca
      781 gggggaaggt taggatgaac aatgtacaga ttttagattg cacacttaga gatgggggct
      841 attgtaacaa ttgggaattt ggatttgata atacaaaaaa aattatttct ggacttatag
      901 aatcaggaat tgagattata gaatgtgggt ttctcacaaa taaaacagta tacaataaaa
      961 atgttacaaa atatacgacc ttagaagaat tagcacagat aattcctgaa aaaagagaag
     1021 gaaaattatt tgtagcaatg atgaactatg gcgaatataa tatagaggat cttccagatt
     1081 atgatggttc atctattgat ggaattagga ttgcgtttca taaaaaaaat tgcaatgaag
     1141 cgttgaaatt atgtcgtgag ataaagaaaa aaggctactt agtttttgtt caagctatgg
     1201 tatctctcaa ttatacagat gaagagtttt tagcagtaat aaaaaatgtg aatcagttta
     1261 aaccatacgc tttttatatt gttgatagtt ttggaatgat gaaaggaaaa gatttaactc
     1321 gtctttttta catgattgag cataatctag aaaaagatat acagataggt tttcactcac
     1381 ataataacat gcagcttgca tattctaatg cacaaaaact tataaccgta caaacaaatc
     1441 gaagcctgat tattgattct tcggtgtacg gaatgggaag aggagcaggt aatcttaaca
     1501 cagaactatt tgtagaatat ttgaatgata atgcaggaaa aaaatatcaa ttaaaaccat
     1561 tattatatat aattgatgaa atactaaata gtttctatca gaagagttat tggggatatt
     1621 cattaccaaa ctatatttca gcagttcata atgctcatcc taattatgca agctatctgt
     1681 catataagaa tactttaaca attgaagcaa tgaatgaaat attcgaaatg atggatgaag
     1741 aaaaaaaggt atcttatgat aaacaatata tagaagattt atactacaaa tatatggaaa
     1801 ctgggaaaat acaggaggaa tatatggaag aactgagaaa atttctctca gacaaaaaaa
     1861 ttctactgat tgcaccagga aaaagttctg tagatgaggt agaaagaata aaagagtttg
     1921 ttaccaaaga agaagtcgta ataattagtg taaattttga atattctcat atagatatag
     1981 attacgtatt tttaagtaat ttaagacgtt ttcatgaatt accgaaagaa agcagaacta
     2041 aatgtattgt tacatcaaat atacaagcat cggatgtata tttacggacg agttataaaa
     2101 agttgttatc tgaaagggaa gttgtaagag acaatgcagg attaatggca attagatttt
     2161 tagccgatat gaatgtggat gaaatattct tagctggatt tgatggatat tcacataatg
     2221 aagatgagaa ttatggtgag caatccatgg aaattataac aaaatttgca ttattagatg
     2281 caatgaacgt gcaaatgaga gatgaattgt cagaattagc taaaaaagta aaggtgacat
     2341 ttttaacgga tccacgttgg gttcgaattg ataagtagta gtaataatgt ttttaaaatt
     2401 ttgataaaga gatagtgaga gaataaaaat gggtaaagtc ttaaataaaa aagaagaaaa
     2461 atatttaagg gacgatgggg cgagatgaaa ctctaatttt gaacttttaa gaatcatttc
     2521 tatattgtgt attgttgctt accatgctaa tttgcgttta aattataatg cagacggaat
     2581 tatagtacat ggttcattaa ctgctaacca gattttcttg aattatattg gtatttgggg
     2641 tcagctagga gttaattgtt ttatatttat ttcggcatat tttttaagtg agaaaagaaa
     2701 cattcacttt gaaaatgttt tgggaattgt attagaatgt attttttata gtgtattagt
     2761 ttatggaatt ctttgtgcat gtggaataga aacattttca ttgaacagct ttgtaaaaga
     2821 aatattatca tttgctctag gtagatattg gtttattaca tcctatttag cattgtatat
     2881 tctaagtccg tatttaaata tcatgcttaa caatctggaa aaacttcagt tgcaaaaaat
     2941 aagtatgtta ttgtttatag gtgtatgctg ttatcgtatg ttatttaaaa catctttgtt
     3001 tggggattgt gctgttttaa ttgcactata tgtcatatgt tatacactga aaaaaacgat
     3061 aataatattt ttgagagaca cagaatcata ggaacaattg tatgtataac agttatacca
     3121 cttctcatat ccataggaat acttttcaaa tttataccaa atgcaatggt taaccagatg
     3181 gttcatattg gttctccatt gatggttata gcaagtatat ttttatttta tgtatttaaa
     3241 aatttttcat tttacagtaa gtatataaat aaacttgcga gaaccagttt aggggtatat
     3301 attttacatg aaaatcatta tttcattgat ggtatgaagt ctagaggctt aatatgggat
     3361 gggatatttg gattaggcaa aaggttttta gatagggatt tttggatttg gtttataatt
     3421 gcagttgtag ttgtgtgcat aatttgcatg gctatcgatg ttgtaagaga aaaatttgaa
     3481 agacagattt ttaaaattaa gagtgtaaag aacctgtgta aacttatgaa tcatgttata
     3541 aatatatacc cataattaat aaatgaggag aaaagaagtg aaagttatat cattcggaga
     3601 attattattg agacttgcag cacccggtta ttcaaagtta tttcaaagag atagtttgga
     3661 gactacattc tgcggagggg aagctaatgt agcggtatcc ttagcaatat ttggatatca
     3721 atcagaattt ataacaaagc ttccggattc agatgttgga tatgcggcag ctaatgcttt
     3781 gcgttatttt ggagtaaatg ttgataatat tatatttgga gagggacgca tgggactgta
     3841 ttatttagaa aaaggagcag ctcagagacc atctaaagta atttatgaca gaaaattttc
     3901 tgcaatttcg ttagctgata gtgatgaatt taagtggaag gagatcctaa acgatgcaaa
     3961 ttggtttcat tggacaggga taaatccagc attgtctgag aatctctttg agacgtcctt
     4021 agatgcatgt caggctgcca aaaaaagagg tataacaatt tcttgtgatt taaattatcg
     4081 atccaagctt tggacatcag aggaagcaaa agaaaagatg gaacagatac taccattggt
     4141 tgatatttgt atttgtaatg aagaagatgc agaaaaagtg ctaggaatta aagctgagca
     4201 tacaaatatt aatagtggag aattaaataa agatggctat gtgcaggtgg ctagggagat
     4261 tcacaagaag tttggatgta ggtatgtagc tacaactctg cgtagaagtt attcggcaag
     4321 tagaaatggt tggagtgcaa tgttatacga agcagaaaaa gataaagcgt atttttccac
     4381 agaatacaac atacaaattg ttgatcgcgt tggtggtgga gacagctttt ctgctggtat
     4441 aatatatgcg ttatgtgaaa atatgtccgg acaaaaggct attgaatttg cgactgcatc
     4501 tagttgttta aaacatacta tagaaggtga ttttaatcga actacaattg aggatgtata
     4561 taatttaatg aatagcggtg gaaatggtcg cgtgttgcgt tagtcaacat ataaagataa
     4621 atcacagtaa tttttatggt gattatctgt cttagtagtg attagaataa cgtgaaaata
     4681 aattgttgag cgtttatact gttttgaaaa ataaatttaa catattgaaa aatacaaata
     4741 tatacgtata ttataaagcg ccgattgtaa aattaagcta gacaactaaa a
//
LOCUS       DARBEG010000130         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000130 DARBEG010000000
VERSION     DARBEG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.135"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SCK20_002630"
     CDS             564..989
                     /locus_tag="SCK20_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146644.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SCK20_002631"
     CDS             1019..1183
                     /locus_tag="SCK20_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146645.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SCK20_002632"
     CDS             2141..3118
                     /locus_tag="SCK20_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1146646.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SCK20_002633"
     CDS             3128..3625
                     /locus_tag="SCK20_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146647.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SCK20_002634"
     CDS             4001..4282
                     /locus_tag="SCK20_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146648.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SCK20_002635"
     CDS             4284..4700
                     /locus_tag="SCK20_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146649.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          817 c          785 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtacacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARBEG010000131         4783 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000131 DARBEG010000000
VERSION     DARBEG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.136"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(182..1144)
                     /locus_tag="SCK20_002636"
     CDS             complement(182..1144)
                     /locus_tag="SCK20_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1146650.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="SCK20_002637"
     CDS             complement(1146..2585)
                     /locus_tag="SCK20_002637"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG1146651.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="SCK20_002638"
     CDS             2770..4716
                     /locus_tag="SCK20_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG1146652.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1527 a          815 c          786 g         1655 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat att
//
LOCUS       DARBEG010000132         4712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000132 DARBEG010000000
VERSION     DARBEG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.137"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            106..357
                     /locus_tag="SCK20_002639"
                     /pseudo
     CDS             106..357
                     /locus_tag="SCK20_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="SCK20_002640"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="SCK20_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1146653.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="SCK20_002641"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="SCK20_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="SCK20_002642"
     CDS             1786..1869
                     /locus_tag="SCK20_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1146654.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="SCK20_002643"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="SCK20_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1146655.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="SCK20_002644"
     CDS             complement(2994..3857)
                     /locus_tag="SCK20_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEG1146656.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="SCK20_002645"
     CDS             complement(3859..4131)
                     /locus_tag="SCK20_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1146657.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="SCK20_002646"
     CDS             complement(4148..4357)
                     /locus_tag="SCK20_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG1146658.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4712)
                     /locus_tag="SCK20_002647"
     CDS             complement(4455..>4712)
                     /locus_tag="SCK20_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEG1146659.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1446 a          796 c          865 g         1605 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc at
//
LOCUS       DARBEG010000133         4634 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000133 DARBEG010000000
VERSION     DARBEG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.138"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            174..518
                     /locus_tag="SCK20_002648"
     CDS             174..518
                     /locus_tag="SCK20_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG1146660.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="SCK20_002649"
     CDS             519..938
                     /locus_tag="SCK20_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG1146661.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="SCK20_002650"
     CDS             complement(1030..1380)
                     /locus_tag="SCK20_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146662.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="SCK20_002651"
     CDS             complement(1346..2566)
                     /locus_tag="SCK20_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG1146663.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="SCK20_002652"
     CDS             2932..3498
                     /locus_tag="SCK20_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1146664.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="SCK20_002653"
     tRNA            3643..3730
                     /locus_tag="SCK20_002653"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="SCK20_002654"
     CDS             4064..>4634
                     /locus_tag="SCK20_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1146665.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DARBEG010000134         4475 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000134 DARBEG010000000
VERSION     DARBEG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.139"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            323..1054
                     /locus_tag="SCK20_002655"
     CDS             323..1054
                     /locus_tag="SCK20_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1146666.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1199..3226
                     /locus_tag="SCK20_002656"
     CDS             1199..3226
                     /locus_tag="SCK20_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1146667.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3334..4431
                     /locus_tag="SCK20_002657"
     CDS             3334..4431
                     /locus_tag="SCK20_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1146668.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1574 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
       61 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
      121 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
      181 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
      241 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
      301 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
      361 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
      421 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
      481 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
      541 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
      601 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
      661 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
      721 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
      781 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
      841 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
      901 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
      961 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
     1021 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
     1081 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
     1141 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
     1201 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
     1261 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
     1321 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
     1381 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
     1441 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
     1501 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
     1561 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
     1621 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
     1681 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
     1741 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
     1801 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
     1861 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
     1921 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
     1981 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
     2041 gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga ttatacctga
     2101 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
     2161 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
     2221 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
     2281 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
     2341 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
     2401 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
     2461 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
     2521 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
     2581 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
     2641 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
     2701 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
     2761 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
     2821 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
     2881 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
     2941 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
     3001 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
     3061 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
     3121 tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa tcggaacctt
     3181 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
     3241 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
     3301 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
     3361 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
     3421 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
     3481 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
     3541 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
     3601 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
     3661 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
     3721 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
     3781 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
     3841 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
     3901 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
     3961 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
     4021 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
     4081 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
     4141 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
     4201 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
     4261 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
     4321 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
     4381 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
     4441 aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DARBEG010000135         4452 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000135 DARBEG010000000
VERSION     DARBEG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.140"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(282..569)
                     /locus_tag="SCK20_002658"
     CDS             complement(282..569)
                     /locus_tag="SCK20_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1146669.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(589..813)
                     /locus_tag="SCK20_002659"
     CDS             complement(589..813)
                     /locus_tag="SCK20_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG1146670.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1268..1438)
                     /locus_tag="SCK20_002660"
     CDS             complement(1268..1438)
                     /locus_tag="SCK20_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146671.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1453..1986)
                     /locus_tag="SCK20_002661"
     CDS             complement(1453..1986)
                     /locus_tag="SCK20_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1146672.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2007..2951)
                     /locus_tag="SCK20_002662"
     CDS             complement(2007..2951)
                     /locus_tag="SCK20_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG1146673.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3588..3674
                     /locus_tag="SCK20_002663"
     CDS             3588..3674
                     /locus_tag="SCK20_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1146674.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3795..>4452)
                     /locus_tag="SCK20_002664"
     CDS             complement(3795..>4452)
                     /locus_tag="SCK20_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146675.1"
                     /translation="ASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPGVIFTLSIVLS
                     GLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCYEEMNDIFVN
                     QEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEACYNLFSDT
                     IPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEWLDQQQQN
                     "
BASE COUNT         1249 a          816 c          681 g         1706 t
ORIGIN      
        1 gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa aaagactagc
       61 aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa ttgttgcaaa
      121 tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg ccatttacta
      181 ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac tgatgaatca
      241 gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca aataccaata
      301 gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa ttctattaaa
      361 gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact acttaatttc
      421 atactaactt caccaacact ttttccctca ctgatttctt tagaataaag aaagagcttt
      481 tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact aattgcattt
      541 aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact accttctagg
      601 tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt ctcctttagc
      661 ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc catagtaagt
      721 agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta atattcctag
      781 aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc gtttttttaa
      841 aattgcttgt tatattaaat aatcttagta attttattat ataacatttt attatataac
      901 aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa aattataaga
      961 tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg cctgtaccaa
     1021 gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct ctttttaagg
     1081 cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg tcatttttta
     1141 aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata ttcatttatt
     1201 attcttttct aaggttaata ataggataat ctctttcaga aagactgctc gtacgagcag
     1261 tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg ttgtagtaat
     1321 taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat aataacattg
     1381 ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt tttgtcataa
     1441 gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg tttgttcgtc
     1501 cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact cttccgtata
     1561 agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca gggtgagttg
     1621 ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga gggcaagttg
     1681 ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg caaccacgtt
     1741 tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca acttatctag
     1801 caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca atacaacctt
     1861 ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat gtttcgatat
     1921 tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg tttcttgtgg
     1981 ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc agataataaa
     2041 ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc ttttttctct
     2101 ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg tttgattttt
     2161 tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa agccaatgct
     2221 ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt tttgatctca
     2281 ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc gtatttcttt
     2341 ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt tataactcgt
     2401 ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag aaagtcttct
     2461 ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa catagtcttt
     2521 gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca ctcgtctaaa
     2581 ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg attttctacg
     2641 tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag cccagactca
     2701 cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa atcttctaca
     2761 aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc aaacgtaaaa
     2821 cgaactgtct tatccccttt ttcacgcatt ctagacacga tggaaaaaaa caaattcatt
     2881 tcaaccggtg taaattttct taaaggaatt gtatttaatt catggtggta ttttacaatc
     2941 tcattagcca tataatcacc tcaatccaat tatgaatgac attttttaaa tgtcaagaca
     3001 ttcatatttg acgttcaaac agacaaaatg tccttcataa acagacaaaa tgtccttcat
     3061 aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataacatt ctgtatccat
     3121 tggtagagta aggacacagc ttacggtaaa gaggtttaaa aagaggttta aaaagagata
     3181 taaaaagagg ggggctaatt ttcccgccta aaaaccactt cttttttctt taaacaggtt
     3241 cgcgcatttt tcaaaactgc caatccaaaa ccttgcttcc tacggtcgcc aaaaaggtgt
     3301 gatcgctacg ctctcacatt gtactgcgca gtcgcctaaa ggctcctttg ctaaaaacat
     3361 gttggtaaag aggcctttga gcggttctga atgcttatct aagccgtttt aagacagtct
     3421 tggataaaaa tccttatcta gctttaaagc gccttacagg cggtcagggc aagccctaac
     3481 aaccctaaaa taagcgatac ttaaaaacac cacacaagtt taatttttta agatataata
     3541 acttgtcgcc tatccgacag tcttgcgact ggaaggaggt gtatctaatg tttgaattgt
     3601 tcatttgccc ccttcttata gaagtttgtg tctctttatt cagttattgg ttgaataacc
     3661 gaaacaagaa gtaaaatcaa taaaggcgac atcaacctga aaaaaagccc cagagaatcc
     3721 gacctctctg gggctttcgt gtatctaatg tttgatcatt tgcatgttta gtttaacatg
     3781 attgccgatc aaattcaatt ttgctgttgc tgatctaacc attccttgac actttttaga
     3841 taatttaatg ccgtttcttt tttcgttgga ttttgtatgt tcgttgcttg ctgaagcagt
     3901 gctttataac gttcgtaggt aactgattgt tgccgttctt ttcgttcctg ttcactagtt
     3961 gaaaaaggga ttgtatcact gaataaatta taacaagctt cttctgtctg cgtatcttta
     4021 aaatattgca cgtctttttt ggttcgctca tagttgtctt gaacctcttt ttgctgtttt
     4081 tctggcaagt tttgataaag acgagtgagt ttttccatgt ttttatccgt caaacgtttt
     4141 tcttgattca caaaaatatc gttcatttcc tcataacaag tggtaaactc ttccattttt
     4201 tgtgttaaca cttgctcctt tttcttttta tcacatgctg cttcatactt ttgagtgata
     4261 tcccctaata aaatacttct tgaaatacgt ttatttccct ctaaaacgcc gaataaacca
     4321 cttaaaacga ttgataatgt aaatataacc cctggcaaca aaaacctctt aaatcgaatt
     4381 ttagagcctt ttaatgaata tcttgtttga taagccttat agctaagcca aataattcct
     4441 aaaatactgg ca
//
LOCUS       DARBEG010000136         4433 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000136 DARBEG010000000
VERSION     DARBEG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.141"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(543..2048)
                     /locus_tag="SCK20_002665"
     CDS             complement(543..2048)
                     /locus_tag="SCK20_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1146676.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="SCK20_002666"
     CDS             complement(2045..2731)
                     /locus_tag="SCK20_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1146677.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SCK20_002667"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SCK20_002667"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG1146678.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DARBEG010000137         4425 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000137 DARBEG010000000
VERSION     DARBEG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.142"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            690..1658
                     /locus_tag="SCK20_002668"
     CDS             690..1658
                     /locus_tag="SCK20_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1146679.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="SCK20_002669"
     CDS             1669..2484
                     /locus_tag="SCK20_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG1146680.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="SCK20_002670"
     CDS             2552..3262
                     /locus_tag="SCK20_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1146681.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="SCK20_002671"
     CDS             3298..>4425
                     /locus_tag="SCK20_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1146682.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DARBEG010000138         4396 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000138 DARBEG010000000
VERSION     DARBEG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.143"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..93
                     /locus_tag="SCK20_002672"
     CDS             <1..93
                     /locus_tag="SCK20_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1146683.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="SCK20_002673"
     CDS             complement(151..1371)
                     /locus_tag="SCK20_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG1146684.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="SCK20_002674"
     CDS             complement(1391..2002)
                     /locus_tag="SCK20_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1146685.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="SCK20_002675"
     CDS             complement(2045..2977)
                     /locus_tag="SCK20_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1146686.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="SCK20_002676"
     CDS             complement(3398..4258)
                     /locus_tag="SCK20_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1146687.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DARBEG010000139         4345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000139 DARBEG010000000
VERSION     DARBEG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.144"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..1853)
                     /locus_tag="SCK20_002677"
     CDS             complement(387..1853)
                     /locus_tag="SCK20_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1146688.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCK20_002678"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCK20_002678"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1146689.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DARBEG010000140         4326 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000140 DARBEG010000000
VERSION     DARBEG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.145"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..283)
                     /locus_tag="SCK20_002679"
     CDS             complement(<1..283)
                     /locus_tag="SCK20_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1146690.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="SCK20_002680"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="SCK20_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="SCK20_002681"
     CDS             complement(2361..2723)
                     /locus_tag="SCK20_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146691.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3065)
                     /locus_tag="SCK20_002682"
     CDS             complement(2727..3065)
                     /locus_tag="SCK20_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146692.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3044..3463)
                     /locus_tag="SCK20_002683"
     CDS             complement(3044..3463)
                     /locus_tag="SCK20_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146693.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="SCK20_002684"
     CDS             complement(3456..3689)
                     /locus_tag="SCK20_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146694.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4326)
                     /locus_tag="SCK20_002685"
     CDS             complement(3686..>4326)
                     /locus_tag="SCK20_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1146695.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1224 a          962 c          620 g         1520 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggat
//
LOCUS       DARBEG010000141         4317 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000141 DARBEG010000000
VERSION     DARBEG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.146"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            149..1060
                     /locus_tag="SCK20_002686"
     CDS             149..1060
                     /locus_tag="SCK20_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG1146696.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1547..1813
                     /locus_tag="SCK20_002687"
     CDS             1547..1813
                     /locus_tag="SCK20_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146697.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            1885..2226
                     /locus_tag="SCK20_002688"
     CDS             1885..2226
                     /locus_tag="SCK20_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146698.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2281..2700
                     /gene="ssb"
                     /locus_tag="SCK20_002689"
     CDS             2281..2700
                     /gene="ssb"
                     /locus_tag="SCK20_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1146699.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            2777..3073
                     /locus_tag="SCK20_002690"
     CDS             2777..3073
                     /locus_tag="SCK20_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146700.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3088..3519
                     /locus_tag="SCK20_002691"
     CDS             3088..3519
                     /locus_tag="SCK20_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1146701.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            3701..4006
                     /locus_tag="SCK20_002692"
     CDS             3701..4006
                     /locus_tag="SCK20_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146702.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         1606 a          638 c          896 g         1177 t
ORIGIN      
        1 tggagtaaaa caagtgtaga ggaaacttga agacttgttc gaacacaaac ttacaagaga
       61 ggggagtaaa gcagttgcag aggaatcagg aagatttgtt cgaacagaaa cttgcaagaa
      121 agcattatta aattgaaggt gatgaaatat gaagtattta ttgcatggct attttattga
      181 ttattctact cgcaatagta gtactttagc gattctatgt ttgcttcatg aattgagagt
      241 ggttactaga aaacagctat acaaactatt gaattttgac agatcaatta gtaaacaagg
      301 agtaacgaat gctttagtga gattatatga aaaacagtta atcgatcgaa aaaaatatgg
      361 aacagaaatg tattattatt tgacgaaaga gggacatcaa agcataggtg ggtattatac
      421 gttgccgaaa gttcctgaat ataatttgaa tcaccattta caaattaaca attatttgat
      481 taaaggaatg gcattggcaa aagaccaccc acatttaaaa cggattattt cagaaagacg
      541 taccgtctat gaaacgaagg atttaaatcc taagacaaaa ggaagaaaat tttttgtgcc
      601 agattttatt tttcgttttc gagacaaaga aaatcatgag atcaattggt cctttgaaat
      661 tgagctaacg ttgaaaacac gtcgtcgcta ccgtgaagcg atttttccga aatatattcg
      721 tgagttgcga acacgtcctg aatcacattt aatttatgtg acacctagtg gcgcaattca
      781 agaagaactt catgcgttta agcgtatttt tttcaagcgc gaaactgaaa aagatgcgag
      841 tcaacaagaa ttgtttgagc ggttgcatat tttttcagct ttagagtttg aagagggatt
      901 aaaagcatta ttagagaatg atccagcagt caattggcgg acagtagaac gagaaaaaat
      961 ttatccgaat ggcgctgttc aaagtcgttt gaatttattt gaagaatcaa ctgaaaatga
     1021 ggaaaaacca aaagtgaagt ttacgcctga agtattgtaa aagagggtgt tcgcttcgct
     1081 ctttttttgt gccagtttgc tggcaccgtt cagacgcaag cgtctgaacc ggctcgtttt
     1141 taggaaggac taacttgttt gatttatccg ttgtcaaaga agtggacgtt cgagagtccc
     1201 taatcgtata gcagtcattc ggcgagtata acctaaaaca taacctgcca attgccaagg
     1261 gtaaaatgca cgctgcgcgc ccttggcaat cgtcagaaca tgttttcgag ccggttatgc
     1321 cgaacgaccg ctatacgaaa agggcctcta aacagaagtc cactttgatc cttgacaact
     1381 gaaacccaaa atcaaacaag caggccttcg tctaaaaacg agccgaatga agaccttggg
     1441 ggttgcaccc ccaaaccccc gtgcccccga cacactaccg aaaaggtggg ggtgggtggc
     1501 gaaaaaatca aataaaacat tctattcaag aaaggaagaa acaaccatga caactaaaaa
     1561 cgagcaactg aaagaacagg caagaaaaga gttgcaagaa agaggtttga ttattgaggg
     1621 gagctttgaa ggagattttg aaacctatat tggttgttat gcaagaccga taaacaaacc
     1681 tacagcctta gacccaatga acgaacaaga agtaaaagaa caagaaaaac atgcagtgaa
     1741 tggttttccg caagatttca ctgaatggta tgagtgggaa attaacaacg ggaagcttga
     1801 aaacttctta taaaagtcag atgacgtgca aagagaatga ttctctttgt acggtaaaaa
     1861 tataaagaaa gaaggaaaaa gaaaatggca aaaaacacac cttatcaagt aggagacatt
     1921 ttttatagta gttggggcta cgaacaaacg aacgtgactt tttggcaaat tgtgaagtta
     1981 acagaaaaaa cagcatggtt tcagcctatt caaaagaaaa cggtcgagca ttattcgtcc
     2041 atgagtggaa aagttgttcc aatgaaagac aagtttatag agtatccttt agcaaaaact
     2101 aaagacagta ttgtaaaaaa acgaattgat ccagaaacct atcctgatat gttatgtggt
     2161 tatcgtagtt gggacacgtt tcatagatat aaaaatcaag ctgtttatga aagtagttat
     2221 tattaagggg atagtttccc ctttcaaaaa taataaatag aagaatggag agaaaaaatc
     2281 atgatgaatt cagtgaattt agtaggacgg ttaacaaaag aagtggattt aaaatacaca
     2341 ccaaaaggca atgcgacagg cacatttatt ttagctgtta atcgaaatta tacgaatgca
     2401 aacggcgagc gtgaagcaga ttatatccgc tgtgtcattt ggcggaaagc tgcggaaact
     2461 ttagcaaaat atactggaaa aggaagtttg attggcatta atggacgtct tcaaacgagg
     2521 agctatcaga atcagcaagg acagaccatg tatgttacgg aagttctagt gacagatttc
     2581 tatttgttag agagtaaaga aatcaatgag caacggtcaa aatcaatggc gaataatcaa
     2641 aataattctt caattccagg aaatgagatt caaatttcag atagttattt gcccttttaa
     2701 gtgaaaaata attttgttct atacctgatt tcttcaggta tagaacaaaa aataaataga
     2761 gatgaggcga ttttttatga aaatcattga cagttacgat caacgaacca tgcaatattt
     2821 gggctcgttt gattatacag atgaaaatat tattaattat gttgcaagtt tacctcctta
     2881 tcaaagcgtt cgcttggtag attttgcaag tgatgaattg gtattaacaa cgatgggcaa
     2941 ctttttagat tatgtcggag acaaaaaatg gttggaaaaa attttgtcta aattgattgc
     3001 gaaacaaaca ggagaagaac ccataaatgc gattcaattg ttcaatcgat atgaacaaga
     3061 aaacaatgat taaagaggag agaaaaaatg aaaagattaa aacgaagtat ttttattgtg
     3121 ctagcattga ttgattttgt tgggacaggt tgtgaagcaa ttgacgttca aaaagctccc
     3181 ttatcggaaa taaaacaagc aataacaaaa caagatgaag aacaaccgct aaatacacaa
     3241 gtagaaatag tgaatcaaaa taaaccgagt ttttctgaag aagatttttc gttagaaaat
     3301 ggaaccaggc aatttttttc agatttggat cgtttaaatc gggtaggtgt agcaagtgca
     3361 cttttgcata aaagcaggat gccaacagaa gaacgagacc gattatacat taagccaact
     3421 ggctggaaac aaaagaaaat gaagaatggc gattatcttt ataatcgttg tcatttgatt
     3481 ggctatcaac gtgcgacaag tttccccata agtatgtaat acgaaacggg aggattaatc
     3541 ttaagattaa tcacaactaa cctatttgac agttggaatg aaagctatca tgtctgctga
     3601 atcaactggt ctgatactcc tacatgcaat aaagtctact aggaagttat tgtatgaagc
     3661 taggtaaagg cggcagaaca atttctaagt gtggaatcat atgaagttgg atatgccggg
     3721 acgctataaa ttactcaggg tgagactgtt tttaattaaa ctgacgaact tgcgaatgta
     3781 cgggtctata accgacttat attcgaaaga tataagattg cgaatgcaaa gaacattatc
     3841 gaaaagtaag aatgttggtc atgaaattcg agatggaaga ctagcggtgt atctgtcaag
     3901 agttggtaaa acatgtttga ttgattcaga tgagagtggg aatgcgacaa aacgttttac
     3961 agaagaaata gagcaactag cagaactgaa tatacgatat ttttaaatac tttagcggag
     4021 aataaaaaaa tcctctacag aaaactacgg agggcaacat gtgctattgt ttcaacaata
     4081 catttttaat ttttaccata acaaaaaagt acttgaaaaa aaacagaaat atgttaaatt
     4141 aaaaatgaaa aaatggaagc ggttacttat tcttttgaat ctaagataaa aattcaaaag
     4201 aataacataa cccaaatatg attatacttg agattctaaa tatttatcga aaaggaattt
     4261 gttttaagga aaaatgattc tatggacggt tattgattta tatttaccaa atggcta
//
LOCUS       DARBEG010000142         4036 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000142 DARBEG010000000
VERSION     DARBEG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.148"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..391)
                     /locus_tag="SCK20_002693"
     CDS             complement(242..391)
                     /locus_tag="SCK20_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146703.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="SCK20_002694"
     CDS             853..1065
                     /locus_tag="SCK20_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146704.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="SCK20_002695"
     CDS             complement(1343..3142)
                     /locus_tag="SCK20_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1146705.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="SCK20_002696"
     CDS             complement(3266..3862)
                     /locus_tag="SCK20_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1146706.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DARBEG010000143         3925 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000143 DARBEG010000000
VERSION     DARBEG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.149"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(573..1130)
                     /locus_tag="SCK20_002697"
     CDS             complement(573..1130)
                     /locus_tag="SCK20_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146707.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="SCK20_002698"
     CDS             complement(1309..1920)
                     /locus_tag="SCK20_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146708.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="SCK20_002699"
     CDS             complement(2033..2290)
                     /locus_tag="SCK20_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146709.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="SCK20_002700"
     CDS             complement(2887..3165)
                     /locus_tag="SCK20_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146710.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="SCK20_002701"
     CDS             complement(3199..3450)
                     /locus_tag="SCK20_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146711.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1195 a          799 c          520 g         1411 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgtat
//
LOCUS       DARBEG010000144         3912 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000144 DARBEG010000000
VERSION     DARBEG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.150"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            60..950
                     /locus_tag="SCK20_002702"
     CDS             60..950
                     /locus_tag="SCK20_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG1146712.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(1023..1847)
                     /gene="nadE"
                     /locus_tag="SCK20_002703"
     CDS             complement(1023..1847)
                     /gene="nadE"
                     /locus_tag="SCK20_002703"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG1146713.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1859..3331)
                     /locus_tag="SCK20_002704"
     CDS             complement(1859..3331)
                     /locus_tag="SCK20_002704"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG1146714.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1152 a          797 c          686 g         1277 t
ORIGIN      
        1 ataactttta ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata
       61 tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa
      121 ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag
      181 aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca
      241 tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg
      301 atattagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta
      361 ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt
      421 ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca
      481 ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg
      541 atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg
      601 acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga
      661 gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc
      721 ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg
      781 gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt
      841 tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag
      901 gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt
      961 ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat
     1021 tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg
     1081 ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa
     1141 tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca
     1201 tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga
     1261 agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca
     1321 tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg
     1381 gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata
     1441 tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca
     1501 tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca
     1561 gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga
     1621 tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct
     1681 gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat
     1741 tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt
     1801 gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct
     1861 atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat
     1921 ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat
     1981 actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag
     2041 gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa
     2101 agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt
     2161 cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat
     2221 ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg
     2281 ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata
     2341 ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat
     2401 caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag
     2461 catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata
     2521 tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt
     2581 ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta
     2641 agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat
     2701 aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag
     2761 cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg
     2821 ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga
     2881 cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca
     2941 gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatc aatggttcat
     3001 ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt
     3061 gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg
     3121 aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg
     3181 cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag
     3241 ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg
     3301 tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca
     3361 tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa
     3421 aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc
     3481 tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag
     3541 acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag attttcctac
     3601 tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac
     3661 ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc
     3721 ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca
     3781 agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac
     3841 aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct
     3901 cgccttgttt tt
//
LOCUS       DARBEG010000145         3878 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000145 DARBEG010000000
VERSION     DARBEG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.151"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1403
                     /locus_tag="SCK20_002705"
     CDS             <1..1403
                     /locus_tag="SCK20_002705"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1146715.1"
                     /translation="LYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQAT
                     FQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVL
                     EHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDP
                     TMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTL
                     NKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGF
                     YHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVI
                     VLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDE
                     IKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPE
                     NEAWLQGALEVFKHEQ"
     gene            1393..2610
                     /locus_tag="SCK20_002706"
     CDS             1393..2610
                     /locus_tag="SCK20_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1146716.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2623..>3878
                     /locus_tag="SCK20_002707"
     CDS             2623..>3878
                     /locus_tag="SCK20_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1146717.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1337 a          664 c          809 g         1068 t
ORIGIN      
        1 tgttgtatcg tgaattactt tcagatgaag aatcaaaaga agatctgatt gccacaatcg
       61 ttgatatttt gggatatgca attgcaccag agtatctttt caacgtctta gcggatcaag
      121 caaaacaagc aacgttccaa ttgaacgatc taaacaaagc ctttgtgcaa ttggcatcta
      181 cctacaatca atttaatggg ttatttgatg atgtggatct ccaatcaaaa aaactaggaa
      241 cagatgagca acaacgaaat gtgacgatta ccgaagtcat caagaaattg aatgatgtag
      301 atgttttaga acacgatggg gacgtgatcg gggatgcgta tgagtttctg atcagtcaat
      361 ttgcttcaga agcagggaag aaagccggag aattttacac acctcatatg gtttctgata
      421 tgatggcgca aatcgtaacg ctcgatcaaa aggaacgacg attctttagt gtttttgatc
      481 caacgatggg ttcaggttca ttgatgttaa acgtacgtaa ctacttaaca cacccagata
      541 acgttaaata tcatgggcaa gaactaaata caacgacata caatctagca aaaatgaact
      601 tgattttaca tggtgtcgac gccgaagaaa tgaatctccg caatggggat acgttgaata
      661 aggactggcc aacagatgaa ccttacacat ttgatgcagt agtaatgaac ccaccttatt
      721 cggctaattg gtcagcagat acgactttct tagatgattc tcggtttaat cgttatggga
      781 aattagcgcc aaaatccaaa gcagattttg cgtttcttct tcatgggttc tatcatttga
      841 aggaaacagg taccatggcc atcgtcttac cacacggagt tttattccgt ggcgctgcag
      901 aaggggtcat ccgtcaaaaa ttactagaag atggcagtat ttatgctgtg atcggtatgc
      961 ctgcgaactt gttctttggg acatcgattc caacgacggt gattgtgttg aagaagaatc
     1021 gtcaaactcg agatgtatta ttcattgatg ctagtcgtga atttgttaaa gggaaaaatc
     1081 aaaataaact atcggaagaa aacattcaaa aaatcctcga aacctacgct gaaagaaaag
     1141 atgtcgagaa atacgctcat ttggcaacct ttgatgaaat caaagaaaat gactataact
     1201 taaatatccc acgctatgta gatactttcg aagaagaaga accaattgat atggttcatg
     1261 tcggaaatga catcaagaag atacgacaag aacagcaagt gctagaaaaa aaacttttag
     1321 aagctatctc ttccctgcaa acaacacctg aaaatgaagc gtggttacaa ggtgcgctgg
     1381 aggtgttcaa acatgagcaa tgatacacaa ccagaaatcc gtttcccagg atttacagat
     1441 gattgggaac agaggaagtt gggggacttc gttgtagatt atgttgaaaa gacaagtgta
     1501 caaaatcaat ttcctatgct aacttcatct caacaaaaag ggatagtttt gcaagaagac
     1561 tattttgcaa atcggcaagt aactacagaa aataatattg gttactttgt actacctaga
     1621 ggatatttca cgttccgtag tcgaagtgat aatgatgtgt ttgtgtttaa ccgtaatgac
     1681 attattgata gagggattat aagctatttt tatcctgtat ttactttaaa atctgctgat
     1741 tccgacttct tcttaagacg tattaacaat ggaatccaac gacaattatc tattcaagca
     1801 gagggaacag gtcaacatgt tctgtctcta aaaaagttta aaaatatagt agcaatgttt
     1861 cctagtgagg aagaacaaca aaaaatcggc accttcttca aacaacttga cgacacgatc
     1921 gctcttcatc agcgtaagtt agatttgctg aaggaaacga aaaaaggatt cttacaaaaa
     1981 atgttccctg aaaacggagc aaaagtgcca gaaatccggt ttcctggttt tacggaagat
     2041 tgggaagagc gtaagttggg agaattaggt aaaactcaat cgggaattgg atttcctgat
     2101 gccgagcaag gtggttctga aggtactcca ttttttaaag tttccgacat gaataacatt
     2161 ggtaatgagt atgaaatgag aaatgcaaat cattatgtat ctaatgagca aattgagcgt
     2221 aaaaaatgga agccaataaa agatgtgcca gcgattatat ttgctaaagt tggcgcagca
     2281 ataatgctta atagaaaacg tttggtcaca agccctttct taatagataa caataccatg
     2341 gcttatttat ttaataatac ttgggatatc tattttggta aaatattgtt tgaaactata
     2401 aatttgccaa gatactccca agtaggggca ttgccttctt ataacagttc tgatattgag
     2461 aatatcagtg ttaaggttcc tgtaaaggat gaacaacaaa aaatcggtac tttcttcaaa
     2521 cagctagatg atacgatcgc tcttcatcaa cgtaagttag atttactaaa agaaacgaaa
     2581 aaaagcttct tacaaaagat gtttgtctag gagggaagaa gaatgaaaat tagtcaccgt
     2641 gaggaagcag aagtcgaaga acagttgatt cgtgtacttg gagaagggca taatcagtgg
     2701 acgtatcgtc cggatctaaa atcagaagaa gacctttggg tcaatttacg ccaaaaaatt
     2761 atctcaaata atcaagcaga actgaatgat tttcctttga ccgataaaga atttgaaacg
     2821 atcaaaacgg aattactgct gcgaacgaaa accccatttg atgctgcgaa atggttaaaa
     2881 ggtgaaaatg ggatggctcg gatcacgatt gaacgggaag attcgcaatt gggttctgtc
     2941 tcacttatcc tttattccaa tcaagacatt ggtggaggaa tctctactta tgaagtggtc
     3001 catcaaattg caaaacgtgg aagtaacata gaagcacggg atcgacgttt tgacgtcacc
     3061 ttattaatca atggattacc gattgtccaa atcgagctga aacaagtaac tgcaaaagat
     3121 ggtgtttatc aggcatttaa ccaaatcaaa aaatatgcag aagaaggtat gtttcggaac
     3181 aatatctttt cgaccttaca gttgtttgtg gtttccaatg aacaaaccac tcggtatttt
     3241 gcaaatgcct taccgaaaga cttgcaccct aagtttttat ttagttggcg aacgaaagac
     3301 aatgaaaaag tagaaaactt atatgagttt tgtaaacaag tgttaaatat cccggatgct
     3361 catcgtttga ttgcggatta tacaatcgtc agtgaagatc aagacaataa aaccctcatg
     3421 gtgttacatc cttatcaagt acatgcgatt caagccttgt ttattgccgc aaataaacat
     3481 caatcagggt atgtgtggca tgcgactggt tcaggaaaga cgttgactag ttttgtatct
     3541 actaagcttt tagcacgaaa atcggggatc gatcgaacga tcatgttagt ggaccgcaaa
     3601 gatttagata atcaaaccac cacagagttt acaaaatttg cttcggaatt taatactggg
     3661 atttcttccg ggaacgccaa agcgaatagc ttgatcgttg gaacgggaag tgccaaagaa
     3721 ttaagtgaaa ccctgttggc agatgccaat gcaaatgttg taatcattac aacccgtcaa
     3781 aaactagatg cggcgttgaa atatgcaaag aaacaagaag agaaaaaagg aaccaatcgc
     3841 ttccaaaaat taatgggcca acatatcgtg tttgtagt
//
LOCUS       DARBEG010000146         3876 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000146 DARBEG010000000
VERSION     DARBEG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.152"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..2257
                     /locus_tag="SCK20_002708"
     CDS             158..2257
                     /locus_tag="SCK20_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1146718.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="SCK20_002709"
     CDS             2453..3316
                     /locus_tag="SCK20_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1146719.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="SCK20_002710"
     CDS             3421..>3876
                     /locus_tag="SCK20_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1146720.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DARBEG010000147         3765 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000147 DARBEG010000000
VERSION     DARBEG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.153"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(684..977)
                     /locus_tag="SCK20_002711"
     CDS             complement(684..977)
                     /locus_tag="SCK20_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG1146721.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1380..2558)
                     /locus_tag="SCK20_002712"
     CDS             complement(1380..2558)
                     /locus_tag="SCK20_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1146722.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2662..2976)
                     /locus_tag="SCK20_002713"
     CDS             complement(2662..2976)
                     /locus_tag="SCK20_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146723.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3083..3301
                     /locus_tag="SCK20_002714"
     CDS             3083..3301
                     /locus_tag="SCK20_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146724.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(3296..3691)
                     /locus_tag="SCK20_002715"
                     /pseudo
     CDS             complement(3296..3691)
                     /locus_tag="SCK20_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         1204 a          721 c          553 g         1287 t
ORIGIN      
        1 ataaaaaata ctcttttttt acgattattg aaaaaaaaag ataaaacttg attaattgaa
       61 gtattgctta aatttggaag ctatgaggga agaataaaag agttaggacg ggattatcta
      121 ctatttttct ttgttttaaa tatttgttat ctttgaacat aaaagagaaa tgtgtcgact
      181 gcagtgttgt gtccctcatc acgaataaac ggtgttcaat tagctgatct tgtattcact
      241 taaaaaatgg taaagtacga gttgctattc cccattttca attttctaac gctcaggaca
      301 aaatactgtt ttcacaacct cattggaata aaagcatata tattgtttca agaacgaaat
      361 cttgttatct ttttaaaggg catctgtcca aaatctaatt ttaggaggta cgttttggta
      421 tttattcgta caaataaaag agaattatct ttattcatgt aacgcatcca attgattttt
      481 tcatgtacgc tcctggaacg attttactga cgattgctta tttaactgcg aatcgctcat
      541 tagcttttat catagctatc catattttga ataacgttct tggctttgta ttatagatac
      601 agatgacaaa aaaagttcaa gtatgcggtg tagaaccaat atcgcatact tgaacttttt
      661 tcaatgagta taaattaaaa cttctaattt tcaattccca tcatctgata tgcccgaccg
      721 tctgaacttc ttttgaaaaa gccaaatgtg attagataac gtctttgttc gacataatcg
      781 ccactagaaa atacgttcag cagtatcgaa ttaacctctg tttctgtata agtcactcca
      841 tggtcaaagc gtttcaagac ttctttcaat aggatgtatt tcttttttgt tgaacttggt
      901 acttgtttta cttttccatc attcatgaat gtgtctaaaa ctttcatctt ttcttgctcc
      961 gtataagtat aaaccatctt ctttctcctt ttgattctaa gaatactcca tttatgttag
     1021 aatattctgg attttttatt aaagcggtta ctcttaagaa agatattatc atagtgctct
     1081 tttttttact actacttata ggtagattta ttctaatcca ttattctccc ctaccaataa
     1141 ttaaagaaaa aactggatca gggtgttcaa attacatgct aagaaaaaag aacccttgaa
     1201 taccaagggt tctttctcag tctgataaca atgattaacg acggatttct ttgatacttg
     1261 tgaaaagtcg ttaaagtatt gatataaaag gatttttatt acctctgact acatattgac
     1321 taatttgtta aaaaatcgtt tttcaagaat cctgtaatac cagtgctttc accgtttttt
     1381 taactctcta aagaactaaa agcaatcaga ggttttacct tttcatttga cgttaagtgg
     1441 cgataaattt tcgaagttgt ttggtcagta tggcctaagc gatctttcaa gacttcccag
     1501 ctacagtgtt ccgctactaa gcttgccatt gtatgacgag ctaaatgagt atgaaatggc
     1561 ttattaattc ctgagcgatt cacgcaccgt ttgacgtgat catttacata ctctcgacga
     1621 taaggaacac cacgtttact agaatcaaga tacacaaaca aatatgtttc ttctataata
     1681 aacctgtaat gttgcctcat ctcacccaaa gccttaatca actgttgtag cttttccata
     1741 actagaggag ttactagaac tcgtctagca gaactaactg tcttagtagt ttcaattcga
     1801 aaatccttac tcttaccttt aactgccgtt tgcttataga acagaatttc atttcttttg
     1861 aaatctatat ctccaggctg tagagcaagc gcctcgctga ctcggcagcc agtgtaaaat
     1921 agaacatgga aaagtacaga catcggtaat tcctcatgaa caatgccata attcaaaaaa
     1981 gttctcaatt catcgacagt aagaaacttg tcctcaatat tatttatggc ttcttttaaa
     2041 tcagttaccg ttaactgata ttttggcatt ctcacagact tcattggatt ttcacttatc
     2101 aaattctttc tttgacaata tttaaaaatc atatttagaa cttgaataag tgattgtaaa
     2161 ctatttttgg catagccaac atttgttcca ttaacgccac attcctttaa ggtaaaaagc
     2221 aattcttgaa ggcactctgg tgtcaaatca gacaactttt tatcaccaat ccatcgaaca
     2281 ataattttta attgttcctc tcgcttatca taggttattt ccttttgttc atttaacgaa
     2341 cggtaatatt ggaaccattg ctcacctaca acactaaact tgtcattgct tgcaacaatt
     2401 tttttcttaa gcctttgttg tttctccatt tccagataag cgagttgcgc ctcacgtttt
     2461 gaattaaagc cacgtcttgt tgttcgatat gcttgaaagt accatgtgat cctaccagta
     2521 gaactgattt gcttcttgaa tcgatctgtt ttttccattt attgattcct cctattttga
     2581 aggaacccaa caagcagaaa ccctttggaa atctttctat ctatgtcatc attgtaccgc
     2641 atcttagaaa gaaaatgtaa cttataaaga tcgaagtagt cgcaattttt ctcgatagtc
     2701 attcgaatca ctctttctca tccattctaa aacatcatca cgaaaatagt gtcctcgaat
     2761 cccgtaccaa cagggaggaa actcaccaag ttttactaaa cggttcagtg tactatcact
     2821 tatctgaaat agtcgcttta aatcagtatt tgttaatacc agaggtaaat gactgactgc
     2881 aagcaatttt tccgtgtaat cttccagttt tttttgtaaa attgaataga actctatttc
     2941 cagagaatta acatttgttt taacttccaa ctccatcacc cttctctctc aaaatcaaaa
     3001 cgaccaccac ggtacccctt aaaattgtaa aaaaacactc cagtactcca tcaaaacgga
     3061 ctagaaaaga ggaattttta atatgaaaca aggaattgca gacataaaaa taattaaaga
     3121 aattcttgaa aaatctacgg ctaatgcaat agcattcgga acaggaatca acttgagtac
     3181 agtaaaaaaa cttaaaagtg gagagagagc tgaagaaaaa ttaaatcttg ccgatgctat
     3241 taaaatcaca gaatttggca tgaaaaatat gccaaccaaa atcgaaatat ggaaatccta
     3301 gctcttccaa agccatttgt cgttggtacc aaacactgcc gagacttttc ccaataatat
     3361 cgccaatttc acgatcatta agaccgatga agaaggacat aaaaatgatt tcttgtcttt
     3421 gactaggtaa tgctgcaatc gcatttgcca actcctcatt ggaaataaag attttcatac
     3481 caaggacaat aaaagtacca ctactattca aatttggttc caaataatag aactctgtac
     3541 ctaacccttt ttgttccaat atttccattg atatttgccg ttggcgaaat ctagcgtact
     3601 tcttttgaat atttaacgcc tcattgcaga ctactttctt acaaaaactg tcaaacgtat
     3661 gttgaatgtg ggtttcctct gagtcgccca taatattctc cccctcaaaa aattcattca
     3721 actcgtttta cttctatccc tttccattat tactacaatc tagga
//
LOCUS       DARBEG010000148         3749 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000148 DARBEG010000000
VERSION     DARBEG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.154"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SCK20_002716"
     CDS             127..579
                     /locus_tag="SCK20_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146725.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SCK20_002717"
     CDS             593..1282
                     /locus_tag="SCK20_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146726.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..>3749
                     /locus_tag="SCK20_002718"
     CDS             1407..>3749
                     /locus_tag="SCK20_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG1146727.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSK"
BASE COUNT         1272 a          491 c          775 g         1211 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaa
//
LOCUS       DARBEG010000149         3712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000149 DARBEG010000000
VERSION     DARBEG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.155"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SCK20_002719"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="SCK20_002719"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG1146728.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="SCK20_002720"
     CDS             complement(2260..2511)
                     /locus_tag="SCK20_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG1146729.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="SCK20_002721"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="SCK20_002721"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG1146730.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="SCK20_002722"
     CDS             3461..3679
                     /locus_tag="SCK20_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146731.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DARBEG010000150         3700 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000150 DARBEG010000000
VERSION     DARBEG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.156"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..717)
                     /locus_tag="SCK20_002723"
     CDS             complement(133..717)
                     /locus_tag="SCK20_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1146732.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="SCK20_002724"
     CDS             complement(928..1131)
                     /locus_tag="SCK20_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146733.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="SCK20_002725"
     CDS             complement(1141..1563)
                     /locus_tag="SCK20_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146734.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="SCK20_002726"
     CDS             1801..2415
                     /locus_tag="SCK20_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1146735.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="SCK20_002727"
     CDS             complement(2580..3404)
                     /locus_tag="SCK20_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146736.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="SCK20_002728"
     CDS             complement(3428..3583)
                     /locus_tag="SCK20_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146737.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
BASE COUNT         1224 a          639 c          525 g         1312 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta
//
LOCUS       DARBEG010000151         3680 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000151 DARBEG010000000
VERSION     DARBEG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.157"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="SCK20_002729"
     CDS             complement(<1..2303)
                     /locus_tag="SCK20_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146738.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="SCK20_002730"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="SCK20_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1050 a          806 c          568 g         1256 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gaatccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacac ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctataatat taccaaataa caaactaact atccatcaat aacgtgtttt attttttaaa
     3601 atttctataa aaaaacaatc attcttaaaa agaatatata aaaataacaa taacctttgt
     3661 gatttttctt gtttgaagaa
//
LOCUS       DARBEG010000152         3583 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000152 DARBEG010000000
VERSION     DARBEG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.158"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            334..1374
                     /locus_tag="SCK20_002731"
     CDS             334..1374
                     /locus_tag="SCK20_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1146739.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            2198..2530
                     /locus_tag="SCK20_002732"
     CDS             2198..2530
                     /locus_tag="SCK20_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG1146740.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3149..3490
                     /locus_tag="SCK20_002733"
     CDS             3149..3490
                     /locus_tag="SCK20_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG1146741.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1322 a          473 c          667 g         1121 t
ORIGIN      
        1 tatattacta gtaatggtag tatgttttat atgcataagg tagttcttaa tttatttttt
       61 gttttgacac tggtagtact taattatctt taattttaaa actaaatttt attcatacgt
      121 ttttctggta gtatttaatt ataaataata tttatttaaa gtagttctta atgactttaa
      181 aggtagtaca taattcattt tatttctgat tttaggtagt atttaattaa aaaacagttt
      241 taatgcgttt gcatttttta tttatctttg ctattttcga aaagagtagg taattttaca
      301 taaataaaac aacgtgaaag tagaggatct acaatgactg attttaaatt ttttaaggcg
      361 gatagagttt acaacgaatt attttatcaa tttccaaaag tctttattgt ttctgacgaa
      421 tacaaaaaaa tgaaagattc aactaagatt gcctatatgc ttttaaaagc aagattagag
      481 atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg tttattttac ttatacgaca
      541 aatgaactat gtagagtatt aaactgccaa aaacaaaaag cgatagcaat caaaaaagag
      601 ttggaatcct ttggtttatt attacaaaag cagatgggat ttaacaaaca gttagggaaa
      661 aataatccta atagactata tctagcagaa ttaaaagtct cagaaaatga tatctactta
      721 ctcgaaaaat ttgatagaga gaatagggaa aacgttgata aatcagaggg tatgaaaatc
      781 atacccaccc tcgacgaaaa atcagacgct gaatcccttg gggctcaaga gggtatgaaa
      841 atcataccgt gccaaaacgt tgataaatca gagggtatga aaatcatacc agaacttaat
      901 aataatatat tagacactaa tagacacaat atagacactg aaaaagaccg cctacaagat
      961 caattgttgt tagacaattt tgagacaatt atgacaaacg acagcattgc tacgtttgtc
     1021 cctgaacgag tattaaattt gataaaaaca ttttcttcaa gttacagtga agctcaaaaa
     1081 accgtccaga ctattcataa tgcaaagaaa aaagctgaaa tagaaagtga tatttcgata
     1141 gtttttgaag aactcgatag ttattatgtc aatgcagaac aagaattata cacgacactg
     1201 ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa cctgattttt
     1261 gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc aagtgaaaaa
     1321 cgtttgaatt atgcaagctc cccaagcact attacgaaag actggttaga gtgaaacttt
     1381 actgacaaag tgattgttgc ttcatggagt aacattaaaa ttaaaaattt tttattattc
     1441 taattatatt acgaatatta caaaataata gctattttaa tagaatttat aagttaaaat
     1501 attatgataa aaatcacaaa cgtagccttt tacgtcataa gtgttaaaag aaataattaa
     1561 tttcaaatat agaaaaggga acgtatgttt gcatactcct tttttttgag ttataataat
     1621 acgcgaagag agataatttc attatttaaa caagctaaaa atcttgatat tacttctttt
     1681 caggcaatac gaatattatt cgcttacatt ttttgtataa atttaattgt ctgctatgct
     1741 tgtgatgaat tgattttcgt tttgcacttt tggatgataa atttggacaa ttgaaaatac
     1801 aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac cttcgtcagc gttcatacac
     1861 ggcattaaga tttggccctt aatgcgttaa cctttcagtg ttacggtatt acccgcaatg
     1921 gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt taaggttaaa
     1981 aatacgtgat attacgatga atctatgcct aagcataatg ttataaaaat gtccgtgtgg
     2041 ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat gcgttccctc
     2101 ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac aagaaaaact
     2161 tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag gaattttgag
     2221 tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa tcacgaagaa
     2281 actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa tccagaaaga
     2341 caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa ttttaaaaga
     2401 tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc aaaacggtgt
     2461 actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga tggggcgttt
     2521 gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct tgcttgaaaa
     2581 tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt agacttgatc
     2641 tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta acgtaaaaaa
     2701 ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg tgtaaataat
     2761 aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag cgtctagatt
     2821 aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa taaggagtag
     2881 gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag tacatgtaga
     2941 tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct actagggttt
     3001 ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt aaacgaaaaa
     3061 cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt aatataaagc atagagaaaa
     3121 tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata tacgattggg
     3181 cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg tgacgtttag
     3241 aaggggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg atttatacga
     3301 tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg atgaaggtaa
     3361 tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg ttgaagatac
     3421 ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg tagcggataa
     3481 agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg agtcacgacg
     3541 attaataaaa aggtagtatt accttaggca ttgagtaagg aat
//
LOCUS       DARBEG010000153         3510 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000153 DARBEG010000000
VERSION     DARBEG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.159"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1097)
                     /locus_tag="SCK20_002734"
     CDS             complement(<1..1097)
                     /locus_tag="SCK20_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1146742.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="SCK20_002735"
     CDS             complement(1227..2603)
                     /locus_tag="SCK20_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1146743.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="SCK20_002736"
     CDS             complement(2603..3259)
                     /locus_tag="SCK20_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1146744.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..>3510)
                     /locus_tag="SCK20_002737"
     CDS             complement(3269..>3510)
                     /locus_tag="SCK20_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1146745.1"
                     /translation="ISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTL
                     GQSYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
BASE COUNT         1088 a          649 c          466 g         1307 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta
//
LOCUS       DARBEG010000154         3294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000154 DARBEG010000000
VERSION     DARBEG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.160"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(552..2165)
                     /locus_tag="SCK20_002738"
     CDS             complement(552..2165)
                     /locus_tag="SCK20_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1146746.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="SCK20_002739"
     CDS             complement(2306..3214)
                     /locus_tag="SCK20_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1146747.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT         1071 a          564 c          576 g         1083 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gaca
//
LOCUS       DARBEG010000155         3224 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000155 DARBEG010000000
VERSION     DARBEG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.161"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            588..1004
                     /locus_tag="SCK20_002740"
     CDS             588..1004
                     /locus_tag="SCK20_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1146748.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            1018..1584
                     /locus_tag="SCK20_002741"
     CDS             1018..1584
                     /locus_tag="SCK20_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG1146749.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            1607..2593
                     /locus_tag="SCK20_002742"
     CDS             1607..2593
                     /locus_tag="SCK20_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1146750.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(2748..>3224)
                     /gene="scm"
                     /locus_tag="SCK20_002743"
     CDS             complement(2748..>3224)
                     /gene="scm"
                     /locus_tag="SCK20_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1146751.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          969 a          605 c          685 g          965 t
ORIGIN      
        1 ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat cgctgggtaa
       61 ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt tcccagtacg
      121 gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag aaaggggctg
      181 agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg cccgagataa
      241 gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt tataaacggt
      301 gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt gaaaagtaat
      361 tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc tcttgttata
      421 aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa acatggaaag
      481 ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac aacctcttgt
      541 ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg agccttaaag
      601 aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg cttttgactg
      661 ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca acagcagaaa
      721 aagaagcggt actgcgtgtg gatggcacag aaatcaaaac attccctttg atggctggag
      781 aagagagcaa cacatatact tacaaagatg aacacggaga ttataacttg atcgagatag
      841 atggcgatcg agtacggata aaagaagcag attgcgatga ccagatctgc gtacgtcgag
      901 gctgggcaac gaaaaatggc gaaaccatcg tctgtttgcc acataaatta gtgatcgaag
      961 tccgttcaac agatggaggt gacgaagaca gtctgattta ttagaccttc acacaccatg
     1021 agtaaattac aaaaaatgat ttatatttcg ctacttgttg cacaaggagt gatcattggt
     1081 ttattggaaa atatgatccc ttatcctttt tcttttgcgc ctggtgcaaa actaggctta
     1141 gccaatctaa tcacgatcgt tgccattttt accatgaaaa aacaagacag cttcttacta
     1201 ctttggctac gactattcct gactactttg ctaggcggaa cgatttccac attcctttat
     1261 agtatgagtg gtgctttgct tagttatttt ggaatgttat tggtcaagca attaggacca
     1321 aaacgtgtca gtatcatcgg aattagtgcg gccggcggtt tcatgcataa tgtcggacag
     1381 cttttgatgg cttctttcat tgcacaatca tggacagtca tgttgtactt gccagtcctt
     1441 tcagttttag ggattttatc agggattgca atcgggatcg cagcgaatta tcttttacga
     1501 catgtgcaga cacttcaacg attccaaaaa gattatgagc agggaaccaa acaaaaatgg
     1561 agcccaatgt ttaccaaaaa ataatcgtca cacaaggagt ttttttatga agctacatcc
     1621 aatgtgggaa aactatcctg ctttaagtaa agaattgaca gaaacactaa aacggatgga
     1681 agaaactgtc cgcctgaaaa acaaacctgt cgaacaagcc atcaaagaaa cgatccatgc
     1741 cggcggaaag ctgttgcgtc ctgcctatca gctattgttc tcacaatttg gaccagagca
     1801 agatcgagac aaagccatcg ctttggcagc ttctatcgaa atgctccata cggccacact
     1861 gatccatgat gatatcgttg atgaagcgga tctgcgacgt ggtcagccta atatccgtag
     1921 tcagtttggt aatgtcgttt ctgtctatgc aggagactat ttatttgtct gctgttttaa
     1981 actattgtct gattactcca cttccttgaa aagtctccaa ttgaattctc gcagtatgga
     2041 aaaagtattg aatggcgaat tagggcagat ggatgatcgc tataattatg aactttctgt
     2101 aaaagaatat ttggaaaata tttcaggtaa gacagctgaa ctgttccaat taagttgttc
     2161 tgttggcgct tttgaaagtg gaacaagcga gcgcttcgca aaaaaagcag gagatatcgg
     2221 actttctatc gggatggcct ttcagattat cgatgatatt ttagattata caaaagagag
     2281 ccaggaaatc ggcaaacctg tactagaaga catgcgtcaa ggggtctatt ctttaccgtt
     2341 gatctactct ttgcaaaaaa acaaaccaaa gctactacct tatttagaga agaaagcggc
     2401 aatgacagaa gaagatgtcg acgccgtacg caaaatcgtt gaacatacag gaggcgtgga
     2461 agaagctagg aagttagcgg cctcttacac agaaaaggct ttgaaggaaa taaagaaatt
     2521 acctgccacc tctcttcgaa caaaagagaa tctcttttcc cttacccaac tgattttgga
     2581 tcgcaaagac tgaataggga aaggttcgat acgtaaataa atttctcaga aggaaatcta
     2641 cgtgtttttt gacaaattga attgtagttc ttgtatttat gataaaaagc aaaaataaag
     2701 agcaacgtcc gttagcgacg ttgctctttt ttatcttgaa tattttttta ccttttcgat
     2761 ttcttataac tcataaaagt gattcttaaa ccgattacaa gtaatataac gcctgcgagt
     2821 gttatccaat tattcgattg acttccagtt ttaggaagtg ctctttttga tttgtttcca
     2881 ttgtcattgc ttgttttatt agttaagtta aagttcgtcc tgttatccga acttgttttt
     2941 gagaaggaga cacttgcatg acgtgtatcg cttgtggtag acgacgtgtc tgtactatcg
     3001 cttgaagttg atgaatctga gctatcactt gtggtagagg atgtatctgt actgtcactt
     3061 gaagctgacg tgtccgtgct atcacttgta gtggtagatg aatctgagct atcacttgtg
     3121 gtagaggtag tatctgtact gtcacttgaa gctgacgtgt ccgtgctatc acttgttgtg
     3181 gtagatgtat ccgtactgtc gcttgaagct gacgtgtccg tgct
//
LOCUS       DARBEG010000156         3221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000156 DARBEG010000000
VERSION     DARBEG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.162"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            301..585
                     /locus_tag="SCK20_002744"
     CDS             301..585
                     /locus_tag="SCK20_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146752.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="SCK20_002745"
     CDS             588..1004
                     /locus_tag="SCK20_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146753.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="SCK20_002746"
     CDS             complement(1141..1860)
                     /locus_tag="SCK20_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1146754.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="SCK20_002747"
     CDS             2535..2630
                     /locus_tag="SCK20_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1146755.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="SCK20_002748"
     CDS             complement(2932..3177)
                     /locus_tag="SCK20_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146756.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DARBEG010000157         3128 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000157 DARBEG010000000
VERSION     DARBEG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.163"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1347
                     /locus_tag="SCK20_002749"
                     /pseudo
     CDS             103..1347
                     /locus_tag="SCK20_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="SCK20_002750"
     CDS             1360..1992
                     /locus_tag="SCK20_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1146757.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="SCK20_002751"
     CDS             2197..2424
                     /locus_tag="SCK20_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG1146758.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="SCK20_002752"
     CDS             2493..2759
                     /locus_tag="SCK20_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1146759.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="SCK20_002753"
     CDS             2749..3018
                     /locus_tag="SCK20_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1146760.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DARBEG010000158         3078 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000158 DARBEG010000000
VERSION     DARBEG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.164"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            752..>3078
                     /gene="esp"
                     /locus_tag="SCK20_002754"
     CDS             752..>3078
                     /gene="esp"
                     /locus_tag="SCK20_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG1146761.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1126 a          458 c          599 g          895 t
ORIGIN      
        1 tttatggatt atcaaaaatt ggatcagtta attgaaagag aaagctggga agattattac
       61 gtgggtctaa ctttttcaga aaagttaaac aactacaagt taatagatta taagtatttg
      121 agtgaaagca attaattatt catgtagagt actagacact ttaaatatgg acaattcaat
      181 tataaagaaa aagaatcaat aatttaatga ggctgtgaca aatgtcattt tttgtttcgc
      241 taaaaacgag tgaaaaaatg ttgataactc caatcattcg tttaagattc taggaatcat
      301 tcagaaatgc tgtgaaaccg atactttata attataaaga aggtgatttc aaattccgaa
      361 gaaggaattt gaagtcacct ttttacttat gtcacagtct cattcagtaa tgcgggataa
      421 ggctccttat tccgttattt gaatactctg aagtttaatc taagaaaggg taatattttg
      481 aaaacatgct tcatcgcata gaaatgaact taatttcaga aagaatacat acatatcttt
      541 ttatatgttt tcagctgctg aatggttaaa aagcacaaat atccgaaaaa aacatgaaga
      601 aatgtacttt ttttgttgta atgttcatga aattctctaa gtaaattagt taacataagg
      661 ttagtaagat tcctattctg tgaataatac gtatcagatt tatcttttat tcttactagg
      721 aaagtaatta ttaagggagt tgatttgaat aatggttagc aagaataata agagagtatt
      781 tctagaaaaa acaaagaaac gagtattgaa gtacagtatc aaaaaattaa gtgttggggt
      841 tgcatctgtt ttgattggtg ttggtcttgt cttgggaaca actgaactag ttaaagcaca
      901 agatgaaatt tctccaagca gcccacttga aacagcgata agctccgtac aagtaggtga
      961 caaagtagct tcggggaata cttttcagga gaatcctggt tatacgaaaa attataactt
     1021 ttcggattta caatttagtc ctcaagaact aactggagac acactaaaag gcaatacaat
     1081 tggatttgaa gtttacggaa aacataatat tgcagcatca actaaaaact gggaaatccg
     1141 tcttcaatta gatgaacgat tagctaaata tgtagaaaaa attcaggtcg atccaaagaa
     1201 aggaattgga tcttctagac ggacctttgt aagaattaat gattcgctgg gtagacctac
     1261 aaatatttgg aaggtcaatt atattcgtgc aagtgatgga ttatttgctg gggcagaaac
     1321 aaccgatacg caaaccgctc ctaatggggt aattacgttt gaaaagagcc tagatgaaat
     1381 ttttaaagag ataggcatag acaatctcaa gaccgatcgt ttaatgtatc gaatctactt
     1441 ggtaagtcat caagacgatg ataaaattgt accgggtatt gatagtacgg gctacttttt
     1501 aacagattca gatgatttct ataatagctt agatgtatct gaaaataatc ctgaccagtt
     1561 taaacatgga tcagtaagtg caaaatatga gaaacctaat actcaaacaa aagatggttc
     1621 aggatccaca ggtgctaatg gagctataat cttagatcat aagttaacga aaaattataa
     1681 tttctcatat tcatcttccg caaagggcac gccttggtat gctaattaca aaattgatga
     1741 acgattagtt ccatatgtag cgggtataca gatgcacatg gttcaggctg acaaagtaac
     1801 atatgatgtt tcgtttgaat ccggaacaaa agtagctgat ttagcaatcg agcgacggga
     1861 aggtcatgaa aattatggca taggttcaat aactaataat gatttaacta aacttatcga
     1921 ttttgctaat gcaagtccac gtccagtcgt tattcgatat gttttacaat tgactaagcc
     1981 attagatgaa atcttagaag atatgaaagc aacagcacaa gttgaagaaa ataaaccatt
     2041 tggtgaagat ttcatctttg attcttggtt gtcggatacg aataaaaaat taatccagaa
     2101 cacttatgga acaggttatt attatttgca agatattgat ggtgatggaa accctgacga
     2161 taaagaagag agcggagaca cgaatccata tatcgggaaa cctgaattag aagaagtata
     2221 tgatgttgac acaacagtta aggggaaagt attcatccac gagttagcgg gaacaggtca
     2281 caaagcccaa cttgttgata aagaaggtac tgtattagca gaaaaaacta tcgctccaaa
     2341 tgaaaaagat ggggctccaa tttcagatac tgtagaattt gaatttacgg gtgtagattc
     2401 aagtaaatta atcgcgaaag atgaattaaa aatccaaatc gtttctccag gttttgataa
     2461 accagaagaa ggttcaaccg ttattaagga atcaccaaaa gcggttgata aacaaaccgt
     2521 ggtagttgga tttaaaccag atgctaaaga atcaattcgg aataataaaa acttacctga
     2581 agatgcagag tattcatgga aaacagagcc tgatacttct aacgttactg atagtacgaa
     2641 aggtattgta actgttaaga tcggaaatcg aactttcgac gtggatgtag agtttgctgt
     2701 aaaagcttct caagctatgg aaaatgatgc aacatacgta cctataacaa caactccaga
     2761 aacgacagtt caaagtggaa aacctacatt tgataaacca gatgttcctt tagctaaaga
     2821 tgccttttca atttcagatg tctataataa gtacttcggt aatgcaagtg ttgatgcaaa
     2881 cactggtatt gttacattca ctccagctaa aggtgtagga gaatcggagc cgattactgg
     2941 aacaattcct attaaaattg tttaccaaga tggttctgta ggcacgaccg atttagcagt
     3001 aactgtaagt aaagatattt atgaaaatcc aggagaaaac attcctgcag gctaccacaa
     3061 agtaaccttc accgcagg
//
LOCUS       DARBEG010000159         3077 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000159 DARBEG010000000
VERSION     DARBEG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.165"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..352)
                     /locus_tag="SCK20_002755"
                     /pseudo
     CDS             complement(1..352)
                     /locus_tag="SCK20_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(612..1160)
                     /locus_tag="SCK20_002756"
     CDS             complement(612..1160)
                     /locus_tag="SCK20_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146762.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1161..2018)
                     /locus_tag="SCK20_002757"
     CDS             complement(1161..2018)
                     /locus_tag="SCK20_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1146763.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2304..2590)
                     /locus_tag="SCK20_002758"
                     /pseudo
     CDS             complement(2304..2590)
                     /locus_tag="SCK20_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2580..2909)
                     /locus_tag="SCK20_002759"
     CDS             complement(2580..2909)
                     /locus_tag="SCK20_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1146764.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          898 a          624 c          389 g         1166 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac ttatttgaga gtcatctaat tggctcataa
      241 tcctttcttt ttggttagta tctgcagtta cttgtttagt atgagccatg aacccaaaca
      301 tactcacccc aacaaccaat cctaaatata ttttcttttt aaactttttc atctacatct
      361 cctcctaata gttttatttt agagttagct ctattactta tttactaact caacaacaga
      421 ttatcataca cttgttaatg aaatcacaat atctcattaa aacacaccct aaaatcataa
      481 ccgttcctat gctcattttt tggatttttt aatcacgtaa ccagaaaaat aaatcccctc
      541 tatttcacgc aaatcaaaga agaactaagt aactaccgtt taaaggtagt tacttagttc
      601 ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag tgagtcttcg
      661 gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc taattctgga
      721 acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt aaacaaatct
      781 tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt tctaagcaat
      841 tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt cgcatttaac
      901 aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt tctgattggt
      961 gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga agtttttatt
     1021 tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc ttgctgcttt
     1081 ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tgttgttccc aaaaatatca
     1141 agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg ttcttccaat
     1201 tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt taagagacct
     1261 ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg cgcatttttg
     1321 agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt atacatttcc
     1381 tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac atataaattt
     1441 gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc atttctcatc
     1501 gattttactt gggtctgtaa gacgacgatt gcccaatccg caatgtacat cgcggattta
     1561 ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc atcaaataat
     1621 ggttctagct tttctttcat atatttgaat tgggataatg aatcattata ttggtctttg
     1681 atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag gttattgatt
     1741 actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt cacagtattt
     1801 tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt catatgcgtt
     1861 aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa ggttcttttt
     1921 ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt acctacgcca
     1981 cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct tttcttttag
     2041 caatattcat ttgatactat tataacatat ttatttttag ttactaccaa aaaaaagata
     2101 tatatgtact accttataat taataagtaa aaggtagtac ataaataagg aaaagctgaa
     2161 atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc gagaagcaga
     2221 gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa gaagccgtcc
     2281 ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata aaagttttca
     2341 tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta agctactcta
     2401 tatgttgtgc ccgcatattt gaacccacta gaccaaatac ctgataaatc cccagttttt
     2461 tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa aaatttttct
     2521 ttcaatggct tctcttttag ctttttaaat acttaatgac atgttttgaa ggaacgattt
     2581 cataagccaa tttctgttgc caactcctct ttagtcatta ctttagaatt agtaagtgta
     2641 tcttcagcaa tatctctaaa ggaagaatgc attttgtttt ttctattctt aaattctaca
     2701 agaatctcat ctttatcgta gccttctgca attatatcag acaaaatatc ttcgctaaaa
     2761 tcataaaatt ctttttgtgg ttcaacaaat tcataaagaa taccattctc taccaacttt
     2821 gcttcaactt cgactccatt aggaacatta aaatctttag gtactgtcaa catgattgaa
     2881 ttgccttgtt ttcttgtttt tgtaatcatt taaatccctc atttctaaca gtatgtaatt
     2941 acttagtaaa tacattatac cacgagataa tgtatttaca agtaattact gtgactcgta
     3001 atgcgtcctt gctgaataaa tttcaaccac gtgttcatct tcatctacct tataaacggt
     3061 tctgttgcaa agttttc
//
LOCUS       DARBEG010000160         2885 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000160 DARBEG010000000
VERSION     DARBEG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.166"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            367..555
                     /locus_tag="SCK20_002760"
     CDS             367..555
                     /locus_tag="SCK20_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146765.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="SCK20_002761"
     CDS             545..805
                     /locus_tag="SCK20_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146766.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            1138..1710
                     /locus_tag="SCK20_002762"
     CDS             1138..1710
                     /locus_tag="SCK20_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146767.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="SCK20_002763"
     CDS             1711..2022
                     /locus_tag="SCK20_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1146768.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="SCK20_002764"
     CDS             2013..2273
                     /locus_tag="SCK20_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146769.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="SCK20_002765"
     CDS             2434..2793
                     /locus_tag="SCK20_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146770.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
BASE COUNT         1012 a          437 c          626 g          810 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttact
//
LOCUS       DARBEG010000161         2826 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000161 DARBEG010000000
VERSION     DARBEG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.167"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            798..1052
                     /locus_tag="SCK20_002766"
                     /pseudo
     CDS             798..1052
                     /locus_tag="SCK20_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1122..1634
                     /locus_tag="SCK20_002767"
     CDS             1122..1634
                     /locus_tag="SCK20_002767"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146771.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="SCK20_002768"
     CDS             1631..2668
                     /locus_tag="SCK20_002768"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1146772.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DARBEG010000162         2776 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000162 DARBEG010000000
VERSION     DARBEG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.168"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            44..439
                     /locus_tag="SCK20_002769"
     CDS             44..439
                     /locus_tag="SCK20_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146773.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            764..1381
                     /locus_tag="SCK20_002770"
     CDS             764..1381
                     /locus_tag="SCK20_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1146774.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1422..1721
                     /locus_tag="SCK20_002771"
     CDS             1422..1721
                     /locus_tag="SCK20_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG1146775.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1755..1922
                     /locus_tag="SCK20_002772"
     CDS             1755..1922
                     /locus_tag="SCK20_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1146776.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1967..2572)
                     /locus_tag="SCK20_002773"
     CDS             complement(1967..2572)
                     /locus_tag="SCK20_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1146777.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          981 a          411 c          506 g          878 t
ORIGIN      
        1 ttaaatatat acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga
       61 aatagaatgg aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt
      121 tatgtttgtt atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta
      181 ctttgcgaat actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc
      241 accttatgat ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga
      301 aatgtggttt gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa
      361 ttatattgta aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata
      421 tagtgttgta atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct
      481 gatgtgaaaa ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat
      541 ttaatggcag accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca
      601 tgcgggagtg taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt
      661 tgtttatgta tggcataata accacataat acaatttgaa acgtttctga tttaatctga
      721 ttgttttgaa acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta
      781 tatccgggtc agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt
      841 aggaatccct gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc
      901 tgaatatcaa ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt
      961 agatagacta ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga
     1021 aaagcagatt gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca
     1081 actagatggg ttagagacgc ttatctctga cttaattctg caattactca gctatatggc
     1141 cgaagacgag cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca
     1201 aaagggtgta aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta
     1261 ttctgactgg aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa
     1321 acgaaatacc ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg
     1381 atagatataa cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa
     1441 gggtcgtttt tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc
     1501 actctttaaa cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg
     1561 gtcagtattc taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc
     1621 gggacttgat tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt
     1681 taattagtca acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa
     1741 ggaggaaaat ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt
     1801 ttaatcgaaa tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt
     1861 atcaaaaaaa ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat
     1921 agaaacgaaa tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg
     1981 ctttctttat tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa
     2041 ctggaatgcc ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat
     2101 ctgttgagtt aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca
     2161 ataatactcc ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac
     2221 ttagcagtga taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa
     2281 aatctaaaaa ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa
     2341 ctgtattttt tatatcttca taatctcttc ctaaacgatc tagtgatgta acgatgacgc
     2401 aatcaccttc acggatataa tttagtaatt tttgaaactc aggacgctca gtatctttac
     2461 cactggcttt ttcttggaat aatttatcaa tgttaagtcc ttccatcatt gccaattgtc
     2521 gatccaaatt ttgatcttgt gaagatactc gaatatagcc aatttttccc ataaaaaact
     2581 cctttttaaa agtgatataa tttacatgta aaatttactt gtagttttac tgctatcata
     2641 gcatttttaa aaataaattt tatgtagact taaaatttac ttcatggagg gtttaaatgg
     2701 atgaaatcaa aagtaacaaa aaagcatttg gcatttattt aggagaacct agtacagaac
     2761 aattaaacca attttt
//
LOCUS       DARBEG010000163         2739 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000163 DARBEG010000000
VERSION     DARBEG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.169"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="SCK20_002774"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="SCK20_002774"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG1146778.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DARBEG010000164         2672 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000164 DARBEG010000000
VERSION     DARBEG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.170"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..322
                     /locus_tag="SCK20_002775"
     CDS             <1..322
                     /locus_tag="SCK20_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146779.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            736..1554
                     /locus_tag="SCK20_002776"
     CDS             736..1554
                     /locus_tag="SCK20_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1146780.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1627..1986)
                     /locus_tag="SCK20_002777"
     CDS             complement(1627..1986)
                     /locus_tag="SCK20_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1146781.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          372 c          471 g          904 t
ORIGIN      
        1 ttataaagca atcattcctg agaacttaga tgaggttaaa ctttatttat gggaagataa
       61 cggagaaatt gtcggtttta gtggtgtaag tgcagaagaa ttagttatgc tatttcttga
      121 tccagatttt attggtaagc gatatggtag tcaaatatta attgagctga ttgaaactga
      181 agatataaaa agaattgatg ttaatagtca aaataaatat gcaaaacatt tttatttaag
      241 ccatggattt gaaattgaat ctgaagaaga cttagatggt tttggtaaac cataccctat
      301 aacgcattta gtaaaaaaat aattcatgaa tggtttatta cctgattgaa catgaataaa
      361 agtaatattt ttattttgat ggcctatttc taggttatga attatccaga tcaaatttgt
      421 gtattacatt cagtaataag gtgcttatct taggatcaac ttatgatact agctgaccaa
      481 ggatgtacca gttatagaaa atcagttaaa gtaaaaatga taaaaatctt atgtcaacac
      541 tgatttaggc tgacctgtgg gttaggagaa agacaggtcg gtagctttta atacaaaaaa
      601 tggcttgtat gtatctagat agttaactga aattagaaat gttcttattt ttaacggagt
      661 aagaaaaata ggctgcttta ggtctgaata ctttttcaat ataatgaaga tatcaaaggc
      721 gacaggagtg tttttatgaa taagaatcct cataatctaa agttggtagc agtagatatg
      781 gatggaacat ttgctcgtac tgattacaca tacgatatcc caaggttcaa ggctgtttta
      841 tcgcgaatga aagctgttga ttgtcaattt gttgttgcaa gtggaaatca atactaccaa
      901 ttgagatcgt tatttccaaa ttattatgat gaattaagtt ttgttgctga aaatggtgct
      961 tttgttaaag ataaaacaga ggtactattt tcagccgata tgcctagaaa agcgatcgag
     1021 gtggtccttg atttatgtaa agactatcct gaagtaaaaa atgtgatgtg tggtctgaat
     1081 agtgcctact gtgaaagagg aaaggtgagt caagcatttt ttgatctaac caatatctat
     1141 taccatagat tagaatgggt agctgatttt gcagaagttc aagataaaat tttaaaattt
     1201 gctccgacta tgcctgttga aaaaaccgaa ttttacgttg aattgttccg tgaaaaatta
     1261 gccgggctat tagtacctac cagtagtggt catggttctg tagacttgat tctttctgag
     1321 tgccataaaa ctttcggctt gaaaatgtta gttgagcgct tggggattaa tcctgaccag
     1381 tgcgttgctt ttggagatgg gggcaatgac attgagatgt tggagtattg tggtctgagt
     1441 tatgaaatgg ataatgcaac ggaagctgtt aagcaagttg ctaagcatca atgtccttca
     1501 aatgacgaag acggtgtttt agtgacgctc gatcgattgt tccctaacac gtaaacacta
     1561 ggtaattcat caaaaaacct cgatataaga aatattcgag gttttaaaac tatatttaga
     1621 acagatttac gaagcgtctg atgagtcaaa atagacttgg acattgctat ctaaaatacc
     1681 actttcattc tttaaacgaa ttgtttgatt tgatggagtt acctctaata ctaaatgccc
     1741 attaataaag gtagtatcgt attcagatgt tttaactgtt gcttcttcgc aaggtattgt
     1801 taaatcatat gctggaatat aatgttgtgt ggaaactaat aaaaaatgat caccaatcat
     1861 acagcatccc tctgctgaag aaaattgatt agagccatca tttaaagtta acaagaatcc
     1921 atcctttaaa cgaggtactt gcttaaatac ttctggttgt acttctaaaa ataaattatc
     1981 gctcattttc acgaccacca tcgacttcca cagttgaaat tgacatttta tttaacacac
     2041 ctattccccc ttaactataa ctcaattcta tataaaaaat attctattct caaataattg
     2101 aacttttcaa atcgccaatt tacttcggct ttatcagagc tcattcatat caagagcgat
     2161 taacgctgta aaaattaaac cgaagatctg ataaattttc tctattttct acaatattta
     2221 aaacgcttcc ttagtctatt atatatctat ttctcttagg agtatgttta gatgtttgaa
     2281 taatagttgc ccaactttaa aggttattct tataatattt tacttcattt tccaatagaa
     2341 aattcaaagg attactggtt tttctaaaat agtttagttt aaatacataa gtatcctccg
     2401 acactgtatt agttcataat catctaaata caaaattttc aaataaaaat atttcaagct
     2461 ctatcgttga cgaatgtaaa cgatacttat aaagtaatag aaattgatta catttgtaaa
     2521 cttaaaatgg ttaatttatc tcacataaaa ctaagaataa aataatgatt aaaagtggtt
     2581 agtttttata cgatttagcc tatcggtaaa aatgaattag agagtgtaaa atattttgtg
     2641 taaatgaaaa aatccataca aaaaaggaag tc
//
LOCUS       DARBEG010000165         2648 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000165 DARBEG010000000
VERSION     DARBEG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.171"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..367
                     /locus_tag="SCK20_002778"
     CDS             <1..367
                     /locus_tag="SCK20_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1146782.1"
                     /translation="QYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSI
                     YSTSARTSKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKV
                     GSFVEVKMPGLMAALKRVY"
     gene            386..1471
                     /locus_tag="SCK20_002779"
     CDS             386..1471
                     /locus_tag="SCK20_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1146783.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            1504..2466
                     /locus_tag="SCK20_002780"
     CDS             1504..2466
                     /locus_tag="SCK20_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1146784.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT         1012 a          306 c          468 g          862 t
ORIGIN      
        1 tcaataccga cctaacaagt tcgaaacatt taaagaattc tattcattag caaatactgt
       61 aacagacgat attaataaag acggtatggc ggcgcttctt ttaaaaggga caacttcagt
      121 tattttttct atttatagta cctctgcaag aacttcaaaa gagaagaaag acgaagcaaa
      181 gcagatattg agtgatccag tgatccgaga agccaagcaa attgctaaga cggattcttc
      241 agcaaaaaaa ataacaaagt tgctttacaa tatgccagct ccactatttt tatctgctgt
      301 aaaagttgga agcttcgttg aagtgaaaat gccagggtta atggctgctt tgaagcgagt
      361 atattagtta atggaggaaa atataatgaa aagaattttg cattttcagg gacgaatggg
      421 tttaggtgga gcagagtctt tcatgatgaa tttatatcgt aaaattgatc gtacaaacta
      481 tcaatttgat tttttgattt atgaagatta tgcagatgtt caagactatc attcagagat
      541 tgaaagatta ggaggccgta tttttgttgt acctaatcct aagaaaaata tttttaaata
      601 tttaatagaa gtgaataagt tactaaaaaa agaatctttt tctatagttc acaatcaggt
      661 ttattttggc gggggaatta atttatggtt agccaaaaag aatggaatca gacagagaat
      721 tgctcatagc catgcaactg aagatggaaa atctcaaaat attgtaatga atgtattgcg
      781 taaattttta accaaattgt tgttacaaaa tgcaacagac taccttgccg ttagtcaaga
      841 agctggtgaa agtctgtttc aaaaccatcc ctttgagata gttcataatg gtattgattt
      901 agagctttat agtaaaaatt cagaagctaa ggttaataag agaaaagaat tggatatctc
      961 aatgagtact tttgttgtgg gaaacattgg acgattagag aaacaaaaaa atcaatctta
     1021 tttgttagag atttttttag aaatactaaa aatgaattct gattcttact tgattttagt
     1081 aggtgaaggg tcacttagaa ataaactgaa agccaaagca gtagaattgg atatagagaa
     1141 aagagtaaaa tttttaggag aaagaaacga tattcccgag ttattatctg tgatggatgt
     1201 ttttgtaatg ccttctcttt atgaaggtct gccaatggtt ggattagaag cacaagcagc
     1261 aaaattaaaa ttagtcttag cagatactat ttccaaagat acaaagttaa caaataatgt
     1321 ggattttgta tcattagatg aaacaccaca agaatgggca aaaaaagtaa taaaagagcc
     1381 gtatgctaat aaaataacac aagaactaaa gagttatgat gtttcgtaca ctactgaaca
     1441 aatggagagt atatatagta gagatcatta aaaatgttca agatatttta ggagtgatta
     1501 tttttgaaaa tatgttattt gatattggca tacaatgata ttggcaactt aaagagattg
     1561 gtcaatcgat tgaacgaaaa tgcagttttt attattcata tagataaaaa aactgatata
     1621 gaaccattta ttgaagcatt taaatcatat gataatgttg aatttttatg taataaaaac
     1681 cgtgttaaaa tttattgggg tggtttttcc attgtacaag cagaaatcaa tttagtaaag
     1741 agagcattac aaaatgagaa atatttaaaa tatgtattat tatctggtgc tgattatcca
     1801 atcaaagata atgagtatat ttataattat tttaaaaaga atagttcagt ggagtttatt
     1861 cgtggaatag atctagatca aattaaacat aaagaatttt attataaaca tatagatgta
     1921 tatcaaaaac atgattatcc tagaattaat aggaataaca caactgcatt taaaattttt
     1981 agagctataa ttaatcgttg tctgaggatg attaaacttc ccccaaaaat tagacaccat
     2041 aaatttgatt tgtatcatgg ttctcaatgg tgggctttgt cgaaagaatg cctaacagaa
     2101 ttaatacaaa tgtatgaaca aaatcaacaa gactacctga attttaaaat aggtatgttt
     2161 gccccagatg aaaaattttt tcatacactt ttttttaatt cctcgttcaa aaataaaaat
     2221 gttattggag gaccagattt accacttgaa ttgaaaaata ttgaagaaac tactttacaa
     2281 acttcgaaat tagcaaatat tcatattatt gatccttcca tgaacaaatg gtttgatgaa
     2341 agggatatag atcaaataaa agaaagtgaa aaattatttg taagaaaagt taaatcggat
     2401 tactctgata aactattaaa tagaattgat aaagaaattt tgaaagttgg aaatatagat
     2461 gaatagaata gtacctgttt tagagaagag tttattaagt cgagatgcgt ttcgagtaat
     2521 aaatttaatc atttgtctgc tttattttat gcctagtact tttaatgtaa ctaatatccc
     2581 gatgaaaata gcttttgctt ggggtggaat tatagttttg tatgaggttt ttataaagaa
     2641 aaattttt
//
LOCUS       DARBEG010000166         2569 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000166 DARBEG010000000
VERSION     DARBEG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.172"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..1026
                     /locus_tag="SCK20_002781"
     CDS             94..1026
                     /locus_tag="SCK20_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1146785.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          943 a          353 c          504 g          769 t
ORIGIN      
        1 cgccgattgt aaaattaagc tagacaacta aaaaagtcat ttgtgctaca ctcaaaatag
       61 ttccgaccaa agaattataa ggagtgagta caaatgacct atacccatct tacaacggat
      121 gaacttgtaa taatagagtc ttatttcaaa atgaatcaat ctgttgctaa aactgcccat
      181 tgcttgaatc gttcaagaca aacgatccat aaagtatatc tgttcttcaa gcaaggaaaa
      241 tcagccttag aatattatca acagtataag aaaaacaaat caaactgtgg tagacgtccg
      301 cttgttttac ccgaggaaca atcagaatat attcaaagaa aggttgttca aggatggaca
      361 cccgatgtga ttgttggtcg tgcagcgttt cctattagtt gttctgctcg taccatttat
      421 cgtatgttca aaaaggggct atttgattct tctgacttac cgatgaaagg caagcgtaaa
      481 ccgaatggac atcaagaaag acgtggaaaa caaactttcc gccgctctat tcatgaacgt
      541 gaaaaggatt atagccaatt ctcaaatgag tttggtcacc ttgaaggtga cactatcgta
      601 ggtctgaaac ataaaagtgc tgtaattacc ttagttgaac gattatcaaa agttatcatc
      661 acattgaaac cgtgtggtag acaagcgatt gatattgaaa aaaaattaaa tcaatggttt
      721 gaatctgtac cgaaaaacct attcaaatcc atcacttttg attgtggaaa ggaattttca
      781 aattggaaac agatcagtaa tgtcaatgat attgccattt atttcgctga tccaggaacg
      841 ccgtctcaaa gaggcctaaa cgagaattct aacggattgt tacgtagaga tggtttattg
      901 aaatctatgg atttcaattc agtagatgaa ttttttattc aatctgtcgc atctaaacga
      961 aataatattc ctagaaaatc actggattat cgaacgatta tcgaacacct ttggaagtat
     1021 ttttgagtta cgtaagtatt gatgatctgt ctaatttaat ttgacaatta agaaagtatt
     1081 aacttgtttt gtgaaaaaga gaatttgaaa aacgattggc gaacaaatgc tcggtaggta
     1141 tggtataaat acagagctaa ttggtgtgat gaaatcagat attcgcgtga gattcagtaa
     1201 agtaggagtt cgaaaacagc taggtgacaa acagtattgc tttggtactt cggaatggtt
     1261 tatcaaattc ttatcaacag ttcacttctc tttatgtttg agtgatgatt cgttaggtta
     1321 ttgcttggaa agaccggatg agagtagaag agtttaattt tataaagtga aaagaggtaa
     1381 tgtacgatat acgtagggct gaaccaatag aggataagca aacgatgtta caggcgcaaa
     1441 attcatagct tactgttttg gagataacag aagaggaaat aatgcagaag taaggcgaaa
     1501 aaaggacgga agcctaacag catatgaaat aaagaaaaat attataacta ctgataaatc
     1561 ggtattgagc aaagtactaa taggagtcaa aaacattcat aagctatgag tgtttcttgg
     1621 ctactttttt atttttatgt atctacaaaa gtttccaggg gcagaacctg tgattattaa
     1681 tggaagaacc tcaagtattg tcttaaatca ttgttatgaa aatggattaa aagagttcat
     1741 caaggtgtga ttaacaagtt acttttcatt acatcgaatt tttcagaagt tgcttatatt
     1801 ggtgatgata ttaatgattt atcatgtatg acatctgtga agaagcggga ggaatagtag
     1861 aatgtccaaa aaatgccaca agaaatgaga ttgaattatc tgattttatt gtaactcaca
     1921 gtggtggtga cgaatctgtg atagagttta ttgagtggat attggaatcg atatagtgta
     1981 gtagtacaga aaaattatca tacaattaat actaggcata agaatattgg catattctag
     2041 agttaaaagt aactattcga aaaggataag gttagaattt tttgttttgc cagtggaaga
     2101 atataaaaag ggcattggaa attaatatat tagaatgcta agcggagaaa aaaatatatg
     2161 aaaagtacgt ccttgtgatg ggatggaaaa atacacttat ttcaaacaac gataatataa
     2221 ttatgtgaac cgaagtatac tcgcttctaa ttcaatcctc aaatcccgac agtcgcgact
     2281 tcaaaaaagc gtatagtttt aatgtatccc catatgatcc gaaaaatggt aatttacaat
     2341 taacagtttg atgatgttga agatgtggaa atgctgaaat taccatgatg agaaacacat
     2401 taattaaagg gaattaatta actagaatat aatctgattc aatttgaatt aagcaaaaaa
     2461 aggctgagtt ttaaataatc gcgaattgta aaattaagtt agaaaaataa aaaggcattt
     2521 atggtacact caaattgtat ttccgacgaa agaaaacaaa ggagtgtaa
//
LOCUS       DARBEG010000167         2533 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000167 DARBEG010000000
VERSION     DARBEG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.173"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..379)
                     /locus_tag="SCK20_002782"
     CDS             complement(<1..379)
                     /locus_tag="SCK20_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1146786.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPS"
     gene            598..1968
                     /locus_tag="SCK20_002783"
     CDS             598..1968
                     /locus_tag="SCK20_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG1146787.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1989..2273
                     /locus_tag="SCK20_002784"
     CDS             1989..2273
                     /locus_tag="SCK20_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146788.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          860 a          380 c          522 g          771 t
ORIGIN      
        1 gactaggaaa actatctata attccgacta gttcaccttt ggtgagcttc ttgatgaaaa
       61 agtatcgaat aatcttgata gtgtaagcta agtttgaact taagcgagtg aatcgactcc
      121 tactaggaaa gtcaccatta agaaataaga cggaacaaac cgctctatac gtggctcttt
      181 ggctagtata gccattaatt atgccccata taacacaagc aataatcact gtatcgcgtt
      241 gcttcagttg atcagtattt cttcggtttt taatcttatc tggtacacaa ttgtggtata
      301 tgttagaaac agttctcata atttccttga atgttataaa agtatctgta taatgttcag
      361 tatattttga tgaacgcata tgtagtcctc atttcttggg agttttgaga ctactatatg
      421 cgttttttgt tgatttacca acatgtttag acattttcct ctaggaaaaa attaactagc
      481 accacgcgta ttgtttatat taaattagat gaccgtgata atgcacagca aatttttgaa
      541 agtatcaatt ctaccggtga aagattgact gcctcggatt tgattcgaaa cttcatcatg
      601 atggataaat ctaatgaaga gcagactacg ctttatacta aatattggcg taggttggaa
      661 gaagtctttg ataattctaa aggaatggaa gactttttca gatactacct tgctgcaata
      721 actggggagt attcagcaaa gcatattctt tatcaagcat tcaaagacta ttggcataaa
      781 gagcgcgatg tatcaagtga tgccgaattg ctccaaaaat tagtgcgata tgctggttat
      841 tttgcgagac tttactataa caaacctgaa ggaaaatact cagaagtatt gagcgatttc
      901 caaagtatgg aaagtatgat gcctgcacct tttgttttgg gattatctga atggtactat
      961 catgaccaat ttatcactga agaccaatat attgatgcga ttaaggtagt gaatgattat
     1021 cagcttagga gatattttaa tggggatgat acaagcagag tgtcaaaagc atttccttca
     1081 tatttgaaaa gtgttaggaa gtacgctaaa gctaatggat atgaaaatat cgttgatatt
     1141 gtaatttatg ttcttgttac tagaaatcaa tcaaataata tggcacttcc atctgacaag
     1201 gcattgaaat ctaatctatt gaatgccaat gcttatgcga tgagattggc ccgttggtta
     1261 cttgaaaaaa tagagaatcg agaaaattca gcaacacttg atatgagtaa cttgtccatt
     1321 gaacacatca tgccacagac tagtacttca tattgggaag agaaggctgg tacatcgggg
     1381 gaagaataca caggattagt aaataccatt ggtaatttaa cactcgttac aaatcctgat
     1441 aattcggccg cgggaaataa agactttgag accaaaaaga aaatctttga agataccctt
     1501 catattcgta tgaataaaga tttgtacgaa ttgactgagt ggacatcatc ggatatttct
     1561 gccagaagtg aagctctaat caatgaactg attactatgt atccctattt gagatcgtcg
     1621 ggcgactatg aacacgatgg gaatagggaa attttcttag aggcgcaagg aataaaagca
     1681 actgggtatt tgaacgaaga cgagaccgtg attatacatt caggttcaga aatttattct
     1741 aagattaaag atattgcatc tgattcgcta gatgaaactc gccaagagct gttggataat
     1801 ggaatcattg aagaaacaat tgggggacta caatttgttc aagattacac tgcctcatct
     1861 gtttcgaatg cagcagctct gctattaggg ggtagccgga atggttggga ctactggaaa
     1921 tatgataacg gaataagtat taatgattcg ctaagaaata agaaatagag catagaggtg
     1981 gtgttaggtt ggataagaac aaactaagat atgcaattct taaattgcgt gatgaaaaaa
     2041 agaatccttt tattgagctt aaagggcaag ttccagaaga agatatttta gaacaaacta
     2101 aatttctttc gcgcaatggc ttactagaat ccatggtttg ggcggataac actgttcata
     2161 tctgggggag tgtttctctg gaaggggaaa aatatttagt tgaaaactca ggtcttgcta
     2221 aggcgtatgc attggcaaaa gaagttaaaa attggattcc attttggggc taagataagt
     2281 ttttaaacgg agaaggtagc aaatcataat tggtttgttg ccttttttga ttgcccaaaa
     2341 agaaaaatga caaagtatag agataaatga catagtacac gggaaaaatg acattgtatc
     2401 accgacaacg tctaaataga tgttgatata tatacgttcc gtacaaataa ttataaaaat
     2461 ttaaatgatt tgagtagaag tatcagtgaa tatctcaagg ggaatagcta tctcaagatt
     2521 cgattttcac ata
//
LOCUS       DARBEG010000168         2526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000168 DARBEG010000000
VERSION     DARBEG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.174"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            222..2375
                     /locus_tag="SCK20_002785"
     CDS             222..2375
                     /locus_tag="SCK20_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG1146789.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          870 a          433 c          552 g          671 t
ORIGIN      
        1 aagtgtttca cgacttaaga aaacataatt ttcaaaaata agtttatttt ttaatgaaaa
       61 aaatagttgg atatgtaact ttaagtgatt gctgtgaaac aaaaagttga ttttatcttg
      121 aaattaggta aagaatttct tgctccgccc attttttctt ttttattaag aagtttttgg
      181 gtacaatgaa gcatgaatgt aaataggcgg aaggatagga aatgatgaaa gattttatga
      241 aaaaaatatt aaatacttct tggttagaaa aaataagaaa aggtgcacaa gaacctaaga
      301 acaaaacgtt tcgccggtcc catgttcctt ttcgcttaaa ttttcttttt ttcattatat
      361 ttacgttatt tgttgcgttg attgctagat tgggttattt gcagatcgtg aacggcgaag
      421 agatggaggc gcgagtgaag tctacttcta cgattacggt ccaagaaagt actcctcgcg
      481 gaatgatcta cgactccaca ggaaaagctt tagttacgaa taaagcgaat caggccatca
      541 catatacgag aggaacacaa gttacggcta aagatttgct tgaaatcgca acgaagctga
      601 actcgctgat cgatgttccg gtagatgaaa atctgacaga tagagataaa aaagattttt
      661 ggctggctga tcccaaacat ttgaaagaag ccacaaaaag actttcggca aaagaaaagc
      721 aattagggaa cagcgaacaa tatagtgcga tggtcaataa agtcacagct gatgagatta
      781 atttaaacga ggagcaatta aaaattgcta ccatctttaa acggatgaat agtgcatatg
      841 aattaaacac tgtctatatc aaaaactctg gtgttactga tcaagagtta gctgttgtgg
      901 cagaacgcgc ttctgaactt ccaggagttt cgactggcac agactggaca cgagaatata
      961 atgcagcaag ttcactgaaa agtattcttg gcagtgtgac gactgaaaag caaggacttc
     1021 cagcagatga agcagagaaa tacctagcaa aaggttattc tcggaatgac cgtgtaggac
     1081 aaagttattt ggaaaaacaa tatgaagatg tcctgcaagg aaccaaaacc caatatgaag
     1141 tttcccttga taatgaagga aatgtttcga atcaaaaaga aatcttctct ggggaaaaag
     1201 gttctaattt aatgttatct atgaatgcag aattccaatc aaaagtagaa gaaattttga
     1261 aaaggaatta tcaaacattg atcaacaacg gcaaagcaca gtattcgcct ggtgcttatg
     1321 cagtggctat gaatcctcag acaggagaag tgctagcaat gactggtttc agtcatgaac
     1381 aaggttccaa agaaatcacc gagaatgctt taggcacgat tactagcgcc tttgatccag
     1441 ggtctgtagt caaggctggt actttgacag caggctgggc aagtaacgcg atcagcggaa
     1501 atcaagtttt gattgatgag ccgattcgtc tgcaaggtgc ttcagaaaaa agctctgtct
     1561 ttaatcgaag cggacaagtt gctcttgatg cagtcaaagc atcggaattg tcttctaata
     1621 cgtatatgat caaagtggcg ttaaagatgt taggactaga ctatacacct ggaatgggac
     1681 tacctagttt agatgaagaa gctaaagctt atcagcaatt gagagactct ttcaaagagt
     1741 ttggtcttgg tacaacgact gggatcgatt tgccgaatga atctcctggg atctcacgtt
     1801 ctgttgatta tatgaagaag ttcaatgcgg ataatggcaa agaatggtat gctccaggta
     1861 actttaccga tttggcattt ggacagtttg acacatatac accaatccaa ttagctcaat
     1921 atgcttcaac agtcgctaat ggcgggaaac gagtacagcc gcacctagtc aaagcgatct
     1981 atggcaatga cgaaaatgga aacttgggtg aagtgaagaa agaaattggc acaacagtag
     2041 aaaatacggt caatatttct gcagaaaata tggcgatcct ccgtgaagga ttccaccaag
     2101 ttgtccatgg taccgatcct tacacaacag ccaaaccatt agccagtgcg aaaatggacc
     2161 tttccgcaaa aacagggact gcagaaacgg ttgcggaagg tcatccagat attgcgacag
     2221 tcaatagtaa tatcgtggca tacggaccaa cagacaatcc ggaaattgcc atcagtgttg
     2281 tcttgccgaa cttattagac gaacaagacc acataaactt gacgattgct aaagaaataa
     2341 tggacgcgta ttatgatatg ttcatggcaa aataaaaaag aggtcgtgaa aaagccgctt
     2401 agtttcgagt atgaagaaaa attttgtgaa aatagcttct cacatttttg cttaaatttg
     2461 acttcatacc gagaaactgg cttttgaaca ccgtttactc ggcaaaaaag gttgtgacaa
     2521 aaattg
//
LOCUS       DARBEG010000169         2485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000169 DARBEG010000000
VERSION     DARBEG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.175"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(869..1387)
                     /locus_tag="SCK20_002786"
     CDS             complement(869..1387)
                     /locus_tag="SCK20_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG1146790.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          815 a          452 c          366 g          852 t
ORIGIN      
        1 ttaaatacac ctacaactta taaattgatg gtatttagat aacacgaaag aataaaaata
       61 acagctgata gtaaagtttg ttgacgattg ctactcaatc gaaagtgagt tagtgcgtat
      121 gaacttgctt ctgaaaactt atgaaatgaa agacctttta catcttgcag aaatcggtac
      181 tgggattaaa ctaagcaata aaaactaatg ttcccctcat tctttgaact tctactattt
      241 gatttattta ttctgataaa tgacgatttc accttcagga tcttcaaatc ccgtatagaa
      301 tttatttaag atctcttcag tatcagtaat aggataggcg ataggccaca taccctttga
      361 tggttagaat aaactgctca aaatctgtat cgtatgttgg ctttagcata ttaacaaagg
      421 ctatctccaa attataaacc ataagcgttg aatcgttctt aacaatttca attcttccta
      481 acccaaagtc agaagatata attaccggac ttgccttacc cttcatgtgg gttgttgaga
      541 ttccatattt aaagggaatt gtcggtacta tcggcggtct gaaaaacatc ccaaaaataa
      601 aaaaatcttt cccctgctag tctcctaagt ccctttgtag cggatatggc accataatct
      661 cattttatcg aagtgagtca tggtttatct gttggccaac caaaattgat acaatctaac
      721 gatgcaccct ctacggcatt ctaggggagt gcacttgcat tcacccaata aaaaccggca
      781 gttagcaaag aacttatgta gtcgtatgtt cttactgtta acatacaaga ggtctccttt
      841 ccacaattgc ttagcagtaa acgggaattt acaaagctaa tctcttccat tttcttatct
      901 ttgttctaaa agtcccaaat ggagcaactg tatttacatg aataaattta tatacttccc
      961 atacagccgt tttggttgcc tcgtctgccc attttctttt gtgtgcagtg aatagatctt
     1021 catcactcat tgaatcaatc atcatgttaa tatcatcaat attacgattg agaatttcct
     1081 ctaattcttt caatggtaga tgtgcatagg tatttgtaaa ccacttatat aactctccta
     1141 actgattcca tttaaattga tcagaaggcg ttttaacaga taatccattt ctttcatcat
     1201 tttcccattt tagaattaaa ttcgtccaac caacttgata tgctaaattt tcagcaggtg
     1261 ttctatccac ttcctcaact cttttatcct tcaattcttc aggaatagtt tcaaattcta
     1321 aaatatattt agtataagtc ctatttattt ctttttttaa ttcttcttta tttttatatg
     1381 ttctcattat attacctcca taaattccta attgtctcct ttaattcatc tataatttca
     1441 tccacgatat tttaatccgt tctacaatgc atatctgata tgcattgact gtctaactca
     1501 tatgtgaatg atttataaaa tatcccaacg tctcatctaa agaaatgttg atcgtcagca
     1561 aggagctaaa aaatacgaat atatttcttt tgtagtaaaa tcggactggt ttgcatatca
     1621 ttttcttcgt aaatatcaaa atcatgttaa ctactatcaa ttactaataa aaactaccac
     1681 tttaagcgtt aagaactgtt aagtttagtt aagtgaggat agttcacaaa atccattgtt
     1741 aaacttcacg aaagtggtaa aactcaaaat gctttatata ggattacagc atcgctcttt
     1801 cttcgattgc gcgttgggta aaaaatatta tgaagtaaaa attgacgatg acacgttcct
     1861 tacggctcat cagatgaaac agctacaaaa acgaaatgat caactcgaag aaaatttaat
     1921 tctaaaaaag cgattgccat attcacgcct cgctcgacga actattaaag gtcaagattt
     1981 acctgaagat catccttttt aggaaattga agctttttat gttgcacaat gcatttataa
     2041 tactacaatg atgttttcac cagatattct tctagggtgc ggtgtaatga aacagataca
     2101 cttgcaaaag aaaattcacc gcgaatttac taaaattatg aataattatg ttaatcttcc
     2161 gaaatttttt tccaaaatta agggatcaag ctggtgttat agattgttta aggttggcaa
     2221 aaaaataact tctttttaaa caattataga aaaccgatac tctgttaagt tggttgacac
     2281 ggtgtcgatt tttatgactt tcttattctg aaacttcact aacggacagt acctaaaaag
     2341 tctatagtat ggacgtttac gagtactatt ttatcaaaaa tgctgctcat ttcaacttat
     2401 atgttttagt gaactacccc cacttagctt ggataggcta caattaacta gacagaaaaa
     2461 ttaaggtgtg tagactagaa gaaaa
//
LOCUS       DARBEG010000170         2436 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000170 DARBEG010000000
VERSION     DARBEG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.176"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..272)
                     /locus_tag="SCK20_002787"
     CDS             complement(<1..272)
                     /locus_tag="SCK20_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146791.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="SCK20_002788"
     CDS             complement(309..446)
                     /locus_tag="SCK20_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1146792.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="SCK20_002789"
     CDS             complement(448..894)
                     /locus_tag="SCK20_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146793.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="SCK20_002790"
     CDS             complement(891..1040)
                     /locus_tag="SCK20_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146794.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="SCK20_002791"
     CDS             complement(1054..>2436)
                     /locus_tag="SCK20_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146795.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DARBEG010000171         2434 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000171 DARBEG010000000
VERSION     DARBEG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.177"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            533..2026
                     /locus_tag="SCK20_002792"
     CDS             533..2026
                     /locus_tag="SCK20_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1146796.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2161..>2434
                     /locus_tag="SCK20_002793"
     CDS             2161..>2434
                     /locus_tag="SCK20_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG1146797.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT          839 a          374 c          490 g          731 t
ORIGIN      
        1 aaattgataa gaattcagac tttataagga atatagtgga tatactaaat atatttgcta
       61 tattcagtat atcagatata ttcaaaatgt caaaatatat ttgtgcgttt tgcgttgaca
      121 atttagagtg aaaagaggta aacttaattc tatagaaacc acagataatt cactatttac
      181 aaagtagcaa aagcagtggt actctttaaa cagaaaaatt acataaaaaa aagagacgga
      241 aataattgat tttagcagaa tcaattattt ctgaatcaca tggttaggac atgtgttcac
      301 gctcgtctca agaatatgta agtagctacc aactacttat attaaaagtt gatcggatac
      361 cttttccgat tgcctttatt ctaccaaaaa aaggcgagca actcaacaga agaacgttta
      421 tttagtgtac ttaaattaaa tattcttaaa cactgaagag agaacacata ctatggctga
      481 gattaaagct aaactgtgtt ctctttttgt ttgagaaagg acgttacaat cgatgttgag
      541 cgaaatttat tcagctattc ttaaaaacgg cttacgtaca tacaaatatc gaaacagcac
      601 catgaaacct gcaggtcata ttgattccag taggagtggt gctatctttg cgtaccgtag
      661 caagcaatta atgaatgttg ggcgtggtat ggtcattact tcggaagaag ctattttaga
      721 gaacgaaaag aaactaacgc attggacacc taatacctat cgctttggta cttatgcgga
      781 tgattatcga acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa tcaacacatt
      841 tgtagttgat atcgattcaa aagagaacca tcaaggcgaa attattttag cctgtttaga
      901 tcaagtgggc tacatgccaa cgctaatttt agaatcagat catggttatc aagtttattt
      961 tgtactaaag acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga tcgatgtagc
     1021 aaaaaaaatt tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg gagtggatct
     1081 aggatgtaac cacttcggaa ttgctcgatt cccacaagaa aataatattg tttttaaaga
     1141 aatgaactat cagtattctt ttgctgactg gattcaatgg tcgatgaagc ttgaatcaga
     1201 tcaaaaaaca aaagaagatc gagaacgaaa actaattgtc tttcctgaga aaaaagaaat
     1261 tcgtcaagta gatgaaccgt ggttcgatat gttacttcat aaagcaaaca tcgtcggcgg
     1321 agaaggacgt cttggtcgaa ataatgtgat tttcacgctt tctttagctt attactcttc
     1381 aggttatgca ttggatactt gcgaatacaa tatgttcaca ttcaacgaac ggttacagga
     1441 accgctatct gaagcagaat tagtaaagat tgtacgtagt gcttattctg gtaattatca
     1501 agcagcaagc agagaattca ttattgaatt gtgccagcaa tgggtttcac caaccttaaa
     1561 aaataatgat ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc cacgagatca
     1621 aagaaaatat agtcataaaa atgaatggaa agaagatcta ttggcttatc taagcgaaaa
     1681 aagtcttgct agtgagcctt atttgaacct gtcaaaaaaa gaattggctt cacaactaac
     1741 gatgcccaag cgaacacttg ataagatctt aaatgagtta cgagaggaga ataaagtctt
     1801 ctacaaggta acacgaggaa gaaatggcgg aatgatcatt gcttcagtca aaacgttgtt
     1861 cgcgagggtt attcaactaa gtaaagaaaa gagactagat tattcaacaa ggatcacaga
     1921 ggtatttgga ctcaacgaga gcttagtaga agagacattt agacaattat caaccccctt
     1981 gaaccctctt aaagaagtga atctttttga agtagataca ggttgaaaaa taaaaggtgc
     2041 aaattgcctt atacaatata tctatgatac ttctgtaggt gttgctccct ttgcctagct
     2101 acgtgtaggt ggtatactta taactgcgta tgcagtctta tgtatcatgg aggaagttgt
     2161 atgtcgttaa aaaattatgt ctattcacat cctgttaatg tgtttatcat tggtcgtttg
     2221 ggattatctg tggaagtgtt ctgtgagctt tatggtttta agcaggggac tttgtcttct
     2281 tgggttacca gggaaaaaac agttgcttct ttgccgattg agtttcttca tgggttgtcc
     2341 ttagcttctg gggagactat ggatcaggta tatgattgtt tgtgtgtgtt agagcaggag
     2401 tatattgagt ataaaatagc taacgagtta agaa
//
LOCUS       DARBEG010000172         2409 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000172 DARBEG010000000
VERSION     DARBEG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.178"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(244..465)
                     /locus_tag="SCK20_002794"
     CDS             complement(244..465)
                     /locus_tag="SCK20_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146798.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(563..808)
                     /locus_tag="SCK20_002795"
     CDS             complement(563..808)
                     /locus_tag="SCK20_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146799.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1048..1662
                     /locus_tag="SCK20_002796"
                     /pseudo
     CDS             1048..1662
                     /locus_tag="SCK20_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2032..>2409)
                     /locus_tag="SCK20_002797"
     CDS             complement(2032..>2409)
                     /locus_tag="SCK20_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1146800.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          842 t
ORIGIN      
        1 tactatatac ttcttcccag aacttgtccg tctccttcaa attatctcta cctctcgagt
       61 tcattgggtg gtttgcccat ttattaaaca tggaatcacc tttctcatac tcatatattt
      121 atgtcctatt tcaattatat ttatatatgc ttaaataaat ttgtatcctg aaatttattg
      181 taatatgaag ctgtatttta catatagtaa ccaaagagtt aataaaataa aaagagcgaa
      241 ccattataga tcagctcttt tttcattgct gtttttaatc ttatctagaa tcacttcaaa
      301 tgtttcaaag gtatttacat ctaactgaca taaccaaagc aagtacttct tttctttgta
      361 agtccaatca atattacggt aagaggacat tagattatgg taccttgctc tttgttcaac
      421 tgaagccaat tcccagcgtt tttcaaatat tttttcttct tccattattg atccctcgca
      481 agacgctacc tcaaacttaa ataatgggca gcgaaagtct aattccttag atctaagtat
      541 agcaaacaaa tatgaaaaaa acttagaagg tagtaaaagt tcttccgtct tttgctttgc
      601 ttctattctt aatgtgtttc gttatcggtg ccctacaaag attttcatta attacacact
      661 catttattgt tgtgtatttt ctgacatttc tctcttgatc aattatttta attccatggg
      721 catcctgata gttcatagga gaggagcggc tatcagaaat agttgcacct atctcttgaa
      781 cttcttttac aacttctgga aaatccatcc atcctgcttc agctgtcaaa agtaataatc
      841 tataatattt atctttcctt ttcaatctaa gaaatcgtca ccccattctt tttcttctat
      901 tataaactat ttaactttta gaattcaaaa accgtttgtc tatttagagt ttaccctaaa
      961 tagacaaaaa tataacattg aaaaatttag agttctaacg tatgattagg gtgtattata
     1021 aacgttatag aaaagtaggg aataaatttg aaaattgctt atattagagt ttcatccatc
     1081 gatcaaaatg aacaacgtca aattgaagaa atgaaaaagt ttggcgctga aaaaattttt
     1141 atcgaaaaac aatctggagc gacgattagt catcggccga ttttccagga agctttagat
     1201 tttgtcagag agcaagatat ttttattgta gaagccattg atcgacttgg acgcaattat
     1261 gacgagatta tcgattcagt gaattatcta aagaagaaga atgtgcgact tattatcacc
     1321 agtttaccga tcatggctga agcaattgga aatcctctcc tagataaatt catcaaagac
     1381 ttaatcattt aaatctcagc catgattgct gaacaagaac gtactgaatc gaaacgacgc
     1441 caggcacaag gaattaaaat cgctaaagca aacggcgtct ataaaggtcg tcctaagtta
     1501 tacagtgcag acaccaaaga tcctcaacgt cgccttgtct ataggagtat cgttcaggat
     1561 cttgagaatg gggttgctat ctcaaagatt gcaacagact acaatgtgac taggcaaaca
     1621 atttaccgaa taaaaaaaga aatcgaccaa ctaattgtat gatttctttt ttgaatcacg
     1681 taattattaa ttagctttgt catcaataca gacaatcatt ttagattcat ctagctaagt
     1741 tgattacatt tatgttaaaa agaatttagt taacataaat ataattgatg cataaatcgt
     1801 ccttatttaa aggcatttaa cattgacaac ttagtataat gtatcttatg taaactaata
     1861 agatttgaat tttattttat acacactcga taatagatca attttatggt cccccgcatt
     1921 tacgattgca caagagaaac ataaagtcga cctattagca tttcttttca attcaaacat
     1981 taaaaaagtt tatacctctg ctactaaaaa atgaaattat ctatttaaaa tttaaaacgg
     2041 taaatctgat tcatccaaat tggaaaatgg atcagcctgt attggagctt tgtatttctc
     2101 cataataaca gatatctttt tttcattttt taaaaaatac cttttaaatt tcttatagtc
     2161 ctctgaaatc aaaatatagc gtgatggata ctcatcaaaa gaagaatcgt attcttcctt
     2221 taaaattccc gatttactta atttttctag tacatcaaat actctatcac tcaagtaact
     2281 ccttaaattg ttatcacttt cccattgaac tacttttcta taagttgaat tatcagttga
     2341 aaagatagaa ttttcatcca taaacgcata tattagcagc aatctttcat cattgtcaaa
     2401 ttctattgg
//
LOCUS       DARBEG010000173         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000173 DARBEG010000000
VERSION     DARBEG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.179"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SCK20_002798"
     CDS             5..1150
                     /locus_tag="SCK20_002798"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG1146801.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SCK20_002799"
     CDS             1321..1779
                     /locus_tag="SCK20_002799"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG1146802.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SCK20_002800"
     CDS             1938..>2407
                     /locus_tag="SCK20_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG1146803.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARBEG010000174         2398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000174 DARBEG010000000
VERSION     DARBEG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.180"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..2069
                     /locus_tag="SCK20_002801"
     CDS             207..2069
                     /locus_tag="SCK20_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1146804.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="SCK20_002802"
     CDS             2069..>2398
                     /locus_tag="SCK20_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1146805.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DARBEG010000175         2374 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000175 DARBEG010000000
VERSION     DARBEG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.181"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            392..976
                     /locus_tag="SCK20_002803"
     CDS             392..976
                     /locus_tag="SCK20_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1146806.1"
                     /translation="MNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFVNGIDINMID
                     NESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISKFMTKLGITL
                     DDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNLRDYILNTKK
                     EVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            969..1997
                     /locus_tag="SCK20_002804"
     CDS             969..1997
                     /locus_tag="SCK20_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG1146807.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            2053..2271
                     /locus_tag="SCK20_002805"
     CDS             2053..2271
                     /locus_tag="SCK20_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146808.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
BASE COUNT          914 a          268 c          419 g          773 t
ORIGIN      
        1 atttaaagaa ttatttaatg ttttcaaaaa acaaaataaa ttactaacac tgggaagctg
       61 tttggcacag gttattttcc aattatggat tggaggaata tttcttattg atgttatttt
      121 attagctaaa ggaacaataa gtattactaa ttttgtaata gtatttatgt acgctaacat
      181 ggtccctact ttatggacta ctttaattag tattatttgg aaatggcaag actattcagt
      241 gtcattaaat agatattacg aatattattc aaataacaac aaagtggctg gcacaaaaaa
      301 aataaataaa ataaattcta ttactggtca atcattaaat ttttcatata acaaaagaaa
      361 ggtattaagc aatataagtt tttcaactga aatgaataaa ctattcatga tatcaggaga
      421 aaatggaaca gggaaaacaa cactgatgaa attatttact aaacagttgc cggttagtaa
      481 taatcaatta ttcgtcaacg ggatagatat taatatgatt gataacgaat cattaagaag
      541 gttaatttca atcgcaacac aaataccata tataattaat agctccgtga aggagaatat
      601 tttaatgggg actaaaaact atgataaaaa aaaattcagt aaattaatag aactattggg
      661 aataagtaaa tttatgacga aattaggtat tactttggac gacaatattt atgatgaaaa
      721 aatattatta tctggaggag aaaaacaaaa aataagttta gctagggcgc ttattaaatc
      781 agcttcagtt attatattag atgaaccatt tagaaattta gataatgaaa ctcgcgataa
      841 tttgagggat tatatactaa atacaaaaaa agaagtttgt tatataatta taactcatga
      901 tcacattcta gataattttt tggattcaaa tgatgaaata ttatatttaa ataaggagta
      961 tgataataat gaatagcaaa aaacttcaga tgggtgaaat tgttagaggt cctagtcaaa
     1021 tatctattga tgttacatat aaatgtaatt ttagatgtaa acattgttac aatgcgagtg
     1081 gtggagacaa ttgttttatg gataatgagt tgactgatga tgaactgtta gaacttacaa
     1141 aagatatatg taatttaaag ccatataata tttgtttctg tggaggagaa actttactga
     1201 gaaaaaaaat agttttatct tgtgccactt tattaaaaga ggcaggtatt cctaatattg
     1261 ctattgttac taatggatca ttagtaactc aggatgttgc aaaagaatta ttcaaagcag
     1321 gtattaatcg atttcaagta agtttagatg gaaaaaatgc tagtagccat gaagcactta
     1381 gattatatcc aaacagtttt gaaaaggcaa ttaatgctat caaattattt agagaattgt
     1441 ccgaagaagt ggatgttgct ttttgtccta ctagttttaa ttacaatcaa ttacgagaag
     1501 tatatcaact agtaaatact cttaaagtga agtctttaag aattcaacca tttatgctta
     1561 gtggtagggg aatgcaaaat agggaaatag tgccaactgt ttctcaatat agaaaactag
     1621 tttctgatat taactatatt aaaagaaatg aagattatac caagatagat tggggagatc
     1681 caattgatca tatcatccga cacagaactt tattgaaaga tttttcgaat tttgtttcta
     1741 ttaggtcaga cggaggatta atggcatcac cttacttgcc aattcaagtt ggaaatatta
     1801 aatgtcacag tctttctaaa tattggcgag caggacttta taaagttttt tctttagatt
     1861 tagttcaaga tatagctact caagtttctt caatacaaga tatgagtaaa gaattaaaaa
     1921 aaggacatat taaatggctt aatgaagata tagaaattga tattctcgat gaatacaaac
     1981 atgatatttt aatttaggtt tatcattgga aagtttagat aaacaaagta cttttagaaa
     2041 ggagggaaaa aaatgatttc tgaacctcaa atgaataata tgtatgaaag cccaactata
     2101 atgggagctg gtgaagctgc gccaagagct tgttcagcaa ttatggtgtg tgtgttatta
     2161 gcatctgcag gagtaggtat ccaagcagga gcggtgtata cttatgctgt agttgctaca
     2221 gtagctgtag ctgcagccgc agtttggaaa acagtaggat tagttggtta ggaattaata
     2281 ggagactaac tgtctcctat tgattccaat gattaagcta aggaggaatt ttaagaaaat
     2341 aatgaaatac aataatttaa aaccgaaatt actt
//
LOCUS       DARBEG010000176         2209 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000176 DARBEG010000000
VERSION     DARBEG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.182"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..>2209
                     /gene="acm"
                     /locus_tag="SCK20_002806"
     CDS             94..>2209
                     /gene="acm"
                     /locus_tag="SCK20_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG1146809.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          867 a          343 c          439 g          560 t
ORIGIN      
        1 attggaaatg agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt
       61 ttatttttag ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta
      121 tcaatgttat ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat
      181 gcaggcagag atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca
      241 gatggaggaa acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca
      301 ggtgattatc tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa
      361 aaagaaatcc cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat
      421 tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg
      481 caatttgaga tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc
      541 accgtgacga gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct
      601 tcttctagcg ttttttatta taaaacagga gacatgctac ctgaagatac taagcacatt
      661 cgatggtttt tgaatattaa caataacggc acttatgttg aacaaccagt taaaatcagt
      721 gatgaaatcc aaagtggaca aagattagat ccaagtacgt ttgaaattaa tcaaatacac
      781 ttaggtgaac agaaggttta tagaggagaa gagggaatcc aacaattttt acaagatttc
      841 cccagcgcta catttaattt ttcagtaact gataattaca ttgaaataac tataccaaaa
      901 aactttgtca atcttaggaa aataatggtg agttataaga cgatcataga aaacccagaa
      961 caaataaatt ttgagaatca ttcggaagct tggtttaaag aatttaataa acctgctgtt
     1021 gatggagaaa gtttcaatca tacagttaaa aatatttcag cttctggtgg agtcaacggt
     1081 acagttagag gcgaattgaa gattttcaaa tatatcaatg acacagagat tggtatccca
     1141 aatgtgactt tcgaattaag acgagcagat gaacagccta ttcaaggaca gtctagtatc
     1201 ctgttgactt cgaatgaaca gggagaagct agtatcaaag gcctgcaagt aggagactat
     1261 gttgtaaaag aaaaagaagc acccaattgg attgattttg atccactaag ttcaaatgaa
     1321 ctcaaattct ccataaacga aaatgataca gagggagtca gtttacctat ttataacaag
     1381 aaaaaggtta cgaacatcac agcaactaaa atttggaacg gtggaaccac tccaagaccg
     1441 agtatttatt ttaaattatt cagagcaagt acaaacaatt gggaaccagt tcctgatgca
     1501 gaaacaaaac gattagacaa tggaataact tcagtgactt gggaagatat ccaacagtat
     1561 gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag tagatcaaaa cggaaatgat
     1621 tatgtccctt caggttatca aaaaattgaa aacgggctag tcgttacaaa tgagaataaa
     1681 gaagtcattt cagtctcagg acaaaaaaca tgggaagaca atgagaacca agacggcaaa
     1741 cgaccgacga cgatcacagt aaatctatta gccgacggtc aaccgatcca acacaaagaa
     1801 gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc tgccaaaata cagagacggg
     1861 caagaaatca tctatactgt aacggaagac aacgtgcccg aatacagtac gacgattgac
     1921 gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa atatatctat accaggtagc
     1981 catataacac cgtggaaaaa ttttccagag aagacagaga gagagaaaat tagaaatcta
     2041 ttctctcaaa aattgcaaat atttgctcat acaggagaat ccagcgaaca aagaagcaat
     2101 tatattagta agagagctgt acctaaacag ataacaaata aatcaaaaag cattcttcct
     2161 aaaacaagta gtaaaaaatc tagttttgct gtaatcattg gtttattga
//
LOCUS       DARBEG010000177         2110 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000177 DARBEG010000000
VERSION     DARBEG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.183"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(86..340)
                     /locus_tag="SCK20_002807"
     CDS             complement(86..340)
                     /locus_tag="SCK20_002807"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1146810.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="SCK20_002808"
     CDS             complement(451..705)
                     /locus_tag="SCK20_002808"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146811.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="SCK20_002809"
     CDS             complement(898..1173)
                     /locus_tag="SCK20_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG1146812.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="SCK20_002810"
     CDS             complement(1145..2098)
                     /locus_tag="SCK20_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1146813.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
BASE COUNT          629 a          389 c          310 g          782 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa
//
LOCUS       DARBEG010000178         2065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000178 DARBEG010000000
VERSION     DARBEG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.184"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SCK20_002811"
     CDS             <1..1008
                     /locus_tag="SCK20_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146814.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SCK20_002812"
     CDS             1025..1174
                     /locus_tag="SCK20_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146815.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SCK20_002813"
     CDS             1171..1617
                     /locus_tag="SCK20_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146816.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SCK20_002814"
     CDS             1619..1756
                     /locus_tag="SCK20_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1146817.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="SCK20_002815"
     CDS             1794..>2065
                     /locus_tag="SCK20_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146818.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DARBEG010000179         2055 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000179 DARBEG010000000
VERSION     DARBEG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.185"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..365
                     /locus_tag="SCK20_002816"
     CDS             <1..365
                     /locus_tag="SCK20_002816"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1146819.1"
                     /translation="CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIR
                     HSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSS
                     IHYLTPQEFEELAKEKMA"
     gene            complement(721..1293)
                     /locus_tag="SCK20_002817"
     CDS             complement(721..1293)
                     /locus_tag="SCK20_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1146820.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1309..1914)
                     /locus_tag="SCK20_002818"
     CDS             complement(1309..1914)
                     /locus_tag="SCK20_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG1146821.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          638 a          425 c          308 g          684 t
ORIGIN      
        1 attgtgtcat tcaaacattg aataaagcaa aaatacatta tcacattcca gaaggaatga
       61 ttctacacac tgacttgggc agccaataca cagcaagaga agtggaacaa tggcttaaaa
      121 ccaacaaaat aaggcattct tatagtagaa aaggaacacc ttatgataat gctggaatcg
      181 aatctttcca tgcctcattg aaaaaagaag aagtctacac gactagctac tcagattttg
      241 aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt ttataaccga aatcgaatcc
      301 atagctcgat tcactatcta accccacagg aatttgaaga attagcaaaa gaaaaaatgg
      361 cttaaccgtc tctactaaag acaatcaaca ccgtagggat atggagataa aatcaataga
      421 gtcaatagat tatttatcga taataacact aaaaaagcca cgttcctaaa cgtgacttac
      481 gctgaacgat atgtgttgtt tggtagacga acaaatcgtt cattataaat atatgatggt
      541 atcctaatat agtaagcgtg gctagcatac ctttaattac tttacctttt acagttacca
      601 tacacatttt ttgagcagaa atcaagtaaa taaaaaaata ttatatttca caacatgcta
      661 atagactcat atctagaacg cattaagtat ttacctgtct ggttcgctga gacttctttt
      721 ttaactttct tcaattttct tctttattct aaaaagcgta ctgcgactga aaccggttat
      781 agcttcaact tccttatacg atttacctga agtaagcagt tcatatgcgt gcctcttctt
      841 cggtgtgatt gttgctttcg gacgtccttc tctgaaatta ggattattct ttcttgcaaa
      901 catttttcct tcttgggtac gttccacaat catatcacgt tccatttctg ctacactcaa
      961 caacgtccgt gtcaccattc gccccatgga cgtattttca atcgttccta gattcaaaac
     1021 cttgatcgtt acgttatcat cgagtaaagg ttcaatgatc tctaatgctt ctctcgtgtt
     1081 acgagcaaat cgatcaagct tcgtaacaac tatttcatct ccaggcttca gaccagccaa
     1141 cagctgattg aattttggcc gatcccgttt tgtacccgta aatttttctg aaaaaatatt
     1201 cttcttctct acgcagttct caagcaactg ttgaatttgt tcttttgtac tttgagaagc
     1261 ggttgagaca cgtgcatatc catatttcgt catttggtcc acctactttt acaaatcatc
     1321 tatcgtatac tcagaatacc cttcatagaa atagctgaca tcgattcctt tcatatataa
     1381 ctcgcgatcg tcaattttat cagtcagagc attggaaatt agatagcgta tttccaaatc
     1441 gtttatggga cttcgctcca ttgcagaaag atagtctgct ttatcgatta agttccaatc
     1501 tacaactttt tgcaattctt cctttaaaat gagatccagc caaattctag tacttcggcc
     1561 atttccttct cgaaatgggt gagcgatatt catctccaca tatttcttga taatctcttc
     1621 aaaagaagtt tgtggcatct gatcaatatg ctgtaacgaa tgttctaaat acataactgg
     1681 tgcaaatcga aaatttcctt ttgcaatatt gaccgatcgg atttttccgg caaaatcgta
     1741 aatatccgaa aatagaaatt catgaatttc agataaacct ttaaatgtac caacttcgat
     1801 ttcatttatt tttccacaat cgtacaattc tttcgctttt tgtttactga gtttttcttc
     1861 aaccttagcc aactcaactt gattggttaa tcccaattta ttctctaaaa tcatgcgaaa
     1921 tctccttaac tttatgacat aagtttatga aacactctca accccattat atcgcgattt
     1981 tgtttatgtg tcaaaaacta ttaagtttct gacccccgat agatttttca agtgtctcta
     2041 ttttgcacag tctag
//
LOCUS       DARBEG010000180         2007 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000180 DARBEG010000000
VERSION     DARBEG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.186"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1872
                     /locus_tag="SCK20_002819"
     CDS             <1..1872
                     /locus_tag="SCK20_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1146822.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          733 a          321 c          412 g          541 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctc
//
LOCUS       DARBEG010000181         2003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000181 DARBEG010000000
VERSION     DARBEG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.187"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SCK20_002820"
     CDS             <1..378
                     /locus_tag="SCK20_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1146823.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SCK20_002821"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SCK20_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1146824.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SCK20_002822"
     CDS             1859..>2003
                     /locus_tag="SCK20_002822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146825.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARBEG010000182         1994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000182 DARBEG010000000
VERSION     DARBEG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.188"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="SCK20_002823"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="SCK20_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1146826.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="SCK20_002824"
     CDS             853..1041
                     /locus_tag="SCK20_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1146827.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="SCK20_002825"
     CDS             1054..1611
                     /locus_tag="SCK20_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1146828.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="SCK20_002826"
     CDS             1643..1885
                     /locus_tag="SCK20_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1146829.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          725 a          308 c          406 g          555 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt cctt
//
LOCUS       DARBEG010000183         1969 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000183 DARBEG010000000
VERSION     DARBEG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.189"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SCK20_002827"
     CDS             <1..177
                     /locus_tag="SCK20_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1146830.1"
                     /translation="LLNNKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWND
                     LYHANEVLKIAEEK"
     gene            178..930
                     /locus_tag="SCK20_002828"
     CDS             178..930
                     /locus_tag="SCK20_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1146831.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            935..1882
                     /locus_tag="SCK20_002829"
     CDS             935..1882
                     /locus_tag="SCK20_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG1146832.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          691 a          263 c          390 g          625 t
ORIGIN      
        1 ttattaaata ataaaataag ttttgaagaa aacattgtta aagaaatgcc tgaatctgct
       61 aatgatatgt atgagaagga gttgaaatat ttctttgaaa acgtagtaac aggcaaagaa
      121 aactggaatg atttatatca tgcaaacgaa gtattgaaaa tagctgagga gaaataaatg
      181 gaaattttat ttactatttg tggtagagct gggtctaagg ggtttaaaaa taaaaatttg
      241 aaatatttgg caggaaagcc cctagtccat ttcgctttag caatgataga tatctatcaa
      301 caaaaacatc ctgaacataa ttgcacgact tgtttgaata cggacagtaa tgagttagta
      361 gaattgacga ctaagttaaa tccgtctgtg aaaattgtag aacgtaaacc tgaattagct
      421 acagatactg tagggaaaat tgaagtgata cgtgattcat tcttacaact agaaagagaa
      481 gataatatat ttgatttaat tatagattta gatttgactt cacctttacg tgaagtttct
      541 aatcttgaag aattaataga tgaacatcta aaaaatttag ataaagacgt ctacttttca
      601 gttgtttctg caagacgtaa tccctatttt aatatggtaa aaagaattga aaatgaagtt
      661 actatagtta ataaatcaag atacacatct aggcaacaga cacctgaagt ttttgaaatg
      721 aatgcctcaa tgtattcttt taaacctgaa tttctaaaaa aaagtaatta tatttttgat
      781 gggcaatgtg ggataataaa aatgagagat tatctaattt tagatattga tagtgaagaa
      841 gattttaaat ggttagaatt tttgtttcct aaatttttag aagattcttc ggagcttcaa
      901 gatatctata attatttata tcggaggtaa aaggatgagc tgttatctta tcaccggtgg
      961 ggctggcttc attggttcta ccttagccaa tcatttagga gaagaacatg tggtaatcgt
     1021 cgtcgatgac ttatcaatgg gaaaagtaga aaatcttgat atgacgaaaa atatcacttt
     1081 tattgaggga gatgtcgctg agaaatcctt gatggaagag atcatgaaag ctaataaatt
     1141 tgattatatc tttcatttag cagcagttgc tagtgtggca gattcagttg cccgaccagt
     1201 agaaacacat cgtgtgaatt ttgaaagtgt attaatgatt ttagaattga tccgcaagta
     1261 tcaacctgat ttaaaacgga tcgtgtttag ttcttcagcg gctgtttatg gggatgaacc
     1321 gactttacca aaaaaagaag agtccgtgat tcgcccattg acgccttatg cggttgataa
     1381 attcgctgca gaacaatatg tgctagatta ttgtcattta tatgatgtac ccggtagcgc
     1441 agttcgtttt ttcaatgtct atggtccaaa tcaaaatccg aattctccat acagtggtgt
     1501 gatttctatt ctcgtagatc gctataagaa acaaatcgta ggagaagcaa cttcatttac
     1561 attatttggt gatggaagcc agtctagaga ctttgtttat atagatgatg taatccaagc
     1621 attactttta gttgcgaatg aggaagctgc actggggaaa caattcaatg ttggaacggg
     1681 taaagcaacc acacttttgg aattgattca tgcaatcaat gaaattctcg gtgtagaatt
     1741 gacacttgaa tacaaagaag agcgttcggg tgatatccat gactctctag cagatattac
     1801 taagatccag tcaattggct atcaaccgaa atatggtatt ttaagtggaa tgactaatta
     1861 tttgaaaact gaaatcaatt aaaacatact aagataagat tagagggtaa attcaggctc
     1921 tttgtcaata aggactgatg agttttgtaa aatcaaatct gggatagaa
//
LOCUS       DARBEG010000184         1949 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000184 DARBEG010000000
VERSION     DARBEG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.190"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1949
                     /locus_tag="SCK20_002830"
     CDS             <1..>1949
                     /locus_tag="SCK20_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1146833.1"
                     /translation="LKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLN
                     SESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSD
                     YEFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVV
                     YVDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKI
                     GENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMI
                     YLALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSV
                     HLAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRN
                     GLLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDR
                     IQRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFE
                     IEFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKG
                     WFAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALS
                     ASLKTSDTADLNIAIISLLEYFGKD"
BASE COUNT          730 a          268 c          326 g          625 t
ORIGIN      
        1 ctaaaagaat cagatttttt tcatggaatt caaaatgggt ggcaagggca ttggattatt
       61 atctctgcga catttgaagg aatcagcgaa gaagaatttg acaatgagat ttgtgcttcc
      121 atttctttaa attctgaaag tcaaacaatc ttagaagaat tgatttcaaa tgcagataaa
      181 ggtgtaggta gtatttctct atttattaga cctaacaaag ctattagaaa gcaattattt
      241 gatatttcca ctgaattaga ctctcatcaa tttaacgaat ttagaaattc tattcgtcta
      301 tccgactatg aattttacta cacatctaaa tcaaatctaa atttctgtat caatgaaaat
      361 tatgataaat tggtaggaag actcaatgac aatatagcta gtaatcctga agaagacgat
      421 gaagctctgt tgggctcaag aattgacatg tcagatattt tcaagcatat ttcggttgtt
      481 tatgttgacg ctcttcgaga tgttcttaga gaaatgaaaa acaatcgaaa cccagtcaaa
      541 agaatcatgg aaacaattga atcaaaaata tcatcagata atgttgagtc attaaaaaca
      601 attatacagc aattaaatga gactattacc agtgttcctg aaattaggaa aattggcgaa
      661 aatattaatc ggcaactaaa tagcataatt ggttcagtgt attctcctaa tcttcttctt
      721 tcctcaacta tgtcagatga tatgggttct ttagcaaaat ttctttcaat gaagccagaa
      781 caaaatatcg acttagactt actagggtta ggacatctaa atatgattta tttagctttg
      841 aaaatagtcg aatttgaagc gtgtcgttca agagaactac ttaatattat gttgattgaa
      901 gaaccagaag ctcatattca tcatcatatt caaaaaactc tgttcgaagg attaaatctt
      961 caaaaaaact atactcagat tctcatgacc acacattctg tacatttagc tgaagtgtct
     1021 gaaatatctc ggatgaatgt attaaaatcg tttaatggca aatctatcgt tatgaatcca
     1081 gcaaataatc tgaactcatt tgctaaagaa aagttaaaaa agaacaatct aaatctaata
     1141 aacgcagtag agagatattt agatgttaaa agaaatggat tgcttttttc taaaggtgtt
     1201 atcttagttg aaggagatgc tgaagaaata cttattcctc aaatagtaaa aaaattattt
     1261 ggtataaact tagacgaact tggaataggt ttgattaatg ttggtagtac ttcgtttgaa
     1321 tatatcgcta gcttgtttga cgatgataga atacagagaa agtgtgctat tgtaactgat
     1381 ttagatgaac aggcaatccc atcagaacat cgattgtata aaagcactgc acaagagaaa
     1441 ggtcaagcaa gaaaagaaaa attatcctcc ctttttaatg aaaacaattg ggttaattgt
     1501 ttttatgcta atacaacatt tgaaattgag tttttagatg tgaataaaga aaatattcta
     1561 acttatgtca gtccaattat agatgacgca ttcaaagata aaaaaacaat aactgcttat
     1621 aaagaagaaa tacaaagtga acaatattta gaaagaaatt tagcgatgtt aaaactagcc
     1681 aatcatgttg gtaaggggtg gtttgcaatt gagttaggtg aaaaaattga ctttttaatc
     1741 aaaattccta agtatattct taatgctatt gcttttgcat gtcaggaaat agtagatctc
     1801 tcaatttata aaaaaattct actccataca atcgacttgt acgaggaaac tacagaattg
     1861 tcagctttat cagctagttt aaaaacaagt gatacagctg atttgaatat agcaataatt
     1921 tctttattag agtactttgg taaagatga
//
LOCUS       DARBEG010000185         1799 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000185 DARBEG010000000
VERSION     DARBEG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.191"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="SCK20_002831"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="SCK20_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1146834.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          506 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaaaaa
//
LOCUS       DARBEG010000186         1786 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000186 DARBEG010000000
VERSION     DARBEG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.192"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1511)
                     /locus_tag="SCK20_002832"
     CDS             complement(45..1511)
                     /locus_tag="SCK20_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG1146835.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          613 a          354 c          310 g          509 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaact
//
LOCUS       DARBEG010000187         1762 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000187 DARBEG010000000
VERSION     DARBEG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.193"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..442
                     /locus_tag="SCK20_002833"
     CDS             <1..442
                     /locus_tag="SCK20_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146836.1"
                     /translation="KYIFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDV
                     RTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMY
                     KLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            439..>1762
                     /locus_tag="SCK20_002834"
     CDS             439..>1762
                     /locus_tag="SCK20_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1146837.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIM"
BASE COUNT          617 a          205 c          318 g          622 t
ORIGIN      
        1 aaaatatata ttttttacgt ctgtttatga ttttgagggc ggagaacctg tcggagaata
       61 tgaattagta tgcaaaatag cagacttagt aggaaaagat aatctaattg ttaagacgca
      121 tcctagagat gtaagaacaa tttataaaga taatggattt atcgaggata aaaattcatc
      181 aataccttgg gaggcgattc aattgtcagg cgattttaac gataaaatat ttatgacaat
      241 taattcagga agtgttattt caggtagtac aatgtctgca aaacctgtac gcacgtatta
      301 tatgtataaa ttgtgtaaca tagaaggaaa tccgtcttgc aaaaagaatg cgtatgatat
      361 tgagcgattg cttaagcaaa aaacgatgaa aaacatttta aagaatgtga aaatagcaga
      421 aagcttagat gatatactat gagcaaaact aaatatttaa ttaaaaatat gggaatatta
      481 acaataagta atttttcttc aaaaatttta gtttttttgt tagtcccttt atatacaagt
      541 gtattatcca caacagaata tggcacatat gatttgacaa tttcaacagc cacattattg
      601 tttccaattt taaccttaaa cattgtagat gctgtgatgc gctttttaat ggataaagat
      661 aaaaataaaa aaaatatagc atctatagcg attaaagtta tatttttaag cgtgatagtc
      721 tttggatccg taatgtattt gttaaatatt acaaaattat ggcaagatgt ttatggatta
      781 gaaaaatata tttttttgta ttatatatcc tatgtatgga atcaatttct aatacaattt
      841 gctaaagggt tagaaaaagt taaagctatg ggcatagcag gtattatcag cacaattgta
      901 acaataattg ccaatatttt cttcttgctt attctgaaat ggggattaat tggttttttc
      961 attgcaaata ttttatcaca gattatttca gctacttatc tgtcagtatt agtgagaata
     1021 tgggaatata tcgattttga ggctaaagat cctcagctaa agagagaaat gctaaattac
     1081 tcgattccat taattgcaac tgttgttgga tggtggatta atagtacagc tgataggtat
     1141 gtggtttcct ttatagtagg tgtctcggcg aatggcttat tgtctgtggc atataaaata
     1201 cctcaaataa ttaatacttt gcagggaata tttatacagg cctggcaaat ttctgcaatt
     1261 aaagaatatg gagataatga gactgcaaaa ttttatggaa atacatttgt tatgattaat
     1321 ttgttaatgt gcataacctg ttcaagtctt atcatattaa ctagaccgtt ttcacatatt
     1381 ctttatgcga aagactttta tgaagcgtgg aaatatgttc catttttatt aatagctaat
     1441 gttttaaatt gtgcatcagg tctgttagga cctatcttat cagcaaaaaa agattctaaa
     1501 tccatgatgt ggtctgcaat cataggggca ttagttaatg tgattatgag tgtagtcctt
     1561 gtttataaga taggggttca aggtgccaca atagccactg ttatatgttc atatgtaata
     1621 tatgttgttc gtaaacgagc tgtgggaaaa gatattttta taaagcatta tcatattgtt
     1681 gtggaatctt ggggattatt gtttgttcag gcaatcatag aaatatattt taaaaattat
     1741 gtaatagaaa ttataattat ga
//
LOCUS       DARBEG010000188         1678 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000188 DARBEG010000000
VERSION     DARBEG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.194"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            316..570
                     /locus_tag="SCK20_002835"
     CDS             316..570
                     /locus_tag="SCK20_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1146838.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="SCK20_002836"
     CDS             571..888
                     /locus_tag="SCK20_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1146839.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1522..>1678)
                     /locus_tag="SCK20_002837"
     CDS             complement(1522..>1678)
                     /locus_tag="SCK20_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1146840.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT          590 a          263 c          317 g          508 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg
     1561 tcgttgtgct cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc
     1621 tagcccttta acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaagaa
//
LOCUS       DARBEG010000189         1672 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000189 DARBEG010000000
VERSION     DARBEG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.195"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(112..489)
                     /locus_tag="SCK20_002838"
     CDS             complement(112..489)
                     /locus_tag="SCK20_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146841.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(789..1670)
                     /gene="galU"
                     /locus_tag="SCK20_002839"
     CDS             complement(789..1670)
                     /gene="galU"
                     /locus_tag="SCK20_002839"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1146842.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          467 a          370 c          245 g          590 t
ORIGIN      
        1 aaaagattct tttatataag tgaaaaaagc ttttttccac caattaaata aatctctcat
       61 ctcaatagtt tatccattat tttatctttt tttgcgtata cctactaaat attaccgtct
      121 tcttctcgtt cttctacttt gttcgagaga atctgtacta gaaaaagtag ttgtatgtct
      181 accttttctg cctatccgag actttctttt catatatggg atataaatga ttagatacag
      241 tacagcaaaa aataaagcaa atacgcaagc ttcccaacta cctaacgcca attcaaccat
      301 caaggtagca accccaacgg atgctgcata ccagataaaa atcagaaacg tttctttttt
      361 tctgaccatg cgaaacatga taaatagact aataagcaat agtggcaaca caaatatcca
      421 attgcttcct tccatcagcc acttgagtat atcaagcagc gactgtgcaa cagcgactaa
      481 ttttcccata gtgaacacac accttttttt ataattaaaa aattcgcctt tttattctac
      541 tatgaatctt ctggcttggc tttcatttct cttatgtaat cattttcttt tatacaactt
      601 aaaaaaaaat taaaccatta gcataatatt ttctgaatct attcacgaaa gtctttttct
      661 atctttcgtt ttccacgact caactgatct agagtcaaaa aactaaaacg acagttgcgc
      721 aaatgacgca caagctgccg ctttaataac aaatagcctt tcattctcag caggccactt
      781 atttccagtt aactttctgt tcttagcttt ttactcaact ggataatata tgcacttaat
      841 gaagtcccaa tttccgaatg ttgaagtcca tattcaatat ttgtcttcat atatcctaac
      901 ttatcgccta cgtcgtatct ttttccttta aactctctcg catagaccgc ctctgtctgg
      961 ttaagagtct gaagtgcatc cgttagctgt atctcgtttc ctgctcctgg ttcttgagtt
     1021 tctaatatct caaatatttt aggagtcagt agataacgtc cgataatggc taaattactt
     1081 ggtgcttttc ccatttcagg tttttctaca aaccctttta ctctgcaacg acccttttcg
     1141 ctatctgttt taggatcaat aattccgtat ttagatgttt cattttctgg tacacgcatg
     1201 acggcaacag cggaagcttg tgtttcttca taatccatca tcaattgttg agtcaatggg
     1261 atatcatctt tcatcaaatc atcccccagc atgacgacaa acggttcatt tcctataaat
     1321 gcttttgctt gaagcacggc atcccccagt cctcttggat gagattgacg gatgaagtga
     1381 agattgactt gagtcgtttc ttcaactaaa gcgagtaatt ctgttttttc cttttcccgc
     1441 aaattgtttt ccaattcgat atttgcatca aagtgatctt caatgggtct tttttctttt
     1501 cccgttacga tcaagatgtc ttcgattccc gaatccaatg cttcttccac aataaactgg
     1561 atcgttggtt tatccacgat tggaagcatt tcttttgcca tggcttttgt tgccggcaaa
     1621 aaacgtgttc ctagcccagc ggcaggaatg actgcttttt tgaccttcac tt
//
LOCUS       DARBEG010000190         1664 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000190 DARBEG010000000
VERSION     DARBEG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.196"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SCK20_002840"
     CDS             131..505
                     /locus_tag="SCK20_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1146843.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SCK20_002841"
     CDS             complement(619..1248)
                     /locus_tag="SCK20_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1146844.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="SCK20_002842"
     tRNA            complement(1382..1454)
                     /locus_tag="SCK20_002842"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DARBEG010000191         1660 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000191 DARBEG010000000
VERSION     DARBEG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.197"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1412
                     /locus_tag="SCK20_002843"
     CDS             <1..1412
                     /locus_tag="SCK20_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1146845.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>1660
                     /locus_tag="SCK20_002844"
     CDS             1546..>1660
                     /locus_tag="SCK20_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEG1146846.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEI"
BASE COUNT          659 a          251 c          355 g          395 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta
//
LOCUS       DARBEG010000192         1630 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000192 DARBEG010000000
VERSION     DARBEG010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.198"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            24..284
                     /locus_tag="SCK20_002845"
     CDS             24..284
                     /locus_tag="SCK20_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146847.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(646..885)
                     /locus_tag="SCK20_002846"
     CDS             complement(646..885)
                     /locus_tag="SCK20_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146848.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1171..1485
                     /locus_tag="SCK20_002847"
     CDS             1171..1485
                     /locus_tag="SCK20_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146849.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          563 a          220 c          287 g          560 t
ORIGIN      
        1 aaaacattaa ggatggatga aatatgaata acgaaactat cattttagga aaaaaatata
       61 tttgccaacc tattggcttg aaacaaaaag tggttggaga agtaatcagt aaattagaaa
      121 attgtgttgt gatctgcgta gacgaatatc atgacgtcga tcgcaatgaa atattagaaa
      181 aatgcggaaa agtagtggca aaatatactc acgtttatga gttggaaaca acagaatatc
      241 tatataacta tagcaacaaa tatgaacata ctttagtttc ataaagattt aaataaagtg
      301 ccttcaaaag cgagaagacc aagcttttga aggcacttta tttagttggc agtgattgtt
      361 catgattttt gatttattca aaaggttcgc ctagagaatc tgtctctgta acgaggcctg
      421 gtggagaaac tcgataaaaa cctatactgc cagaactgtc ctattttcgg atagacaaac
      481 tagtagtttt aaaaagatca tccgatccaa tcatttcaaa aaatgcaaaa ctgatatcag
      541 gatcatttag ctcaggcttg ttatcgtaaa gcatttgtaa aggttgcatt tagttaggag
      601 taaacgcgaa actatcatca ttatttgctg aagaagatga agaagctaat tcagagtttg
      661 tttgaaataa aggagacgtg ctgacagttt ctttaccctg ttttttgcag aggtagaaac
      721 ttttatatcg gaagtttcta ccggagaatt tttctcattt gtagaaattg ttgaatcgat
      781 actattcgaa atttgcgtat ctgaaggtat gttctttgat gcagaagtcc cacaagcagt
      841 aagtaacaat aaatttgata aactgataaa cagaattctt ttcattatgt acacactcct
      901 tatagtatcg tttagcatac acctatgctc agtattaggc aaaatcaatg caaaactatc
      961 aagtctttta tagtatctat tagtcttagt aatacatata tggagagggt gatatcccag
     1021 ttcaaaccag atacattggt taccatctgt attaacgatt atgcctctaa acgctttttt
     1081 tatgaaaaaa tttataagtg aaaacgtagg tatgatatta tctagaggta atttttttgg
     1141 gtatttaaat agttgctagg aggatagctt ttggattttt acagtaattt tatcttaata
     1201 atagcaatat tattacttct aaatatttgg ttttttgata agagtagaaa tgcagggata
     1261 ggttttagaa ccaaacgaag tacgtcttct gaaaaaaagt gggtatattc acagactatt
     1321 ttttacggag gggttatttc aatcagttta ctttcttcaa cactttattc ttttaatgtt
     1381 atagacgttt ccatgtcaaa ttttatatct attattggaa tccttatttc tgcaattatt
     1441 acacaactac ttcttgtgtt tgaagagaag tcaaaaaata attaaagcaa ttatgccttt
     1501 tagtacagga attattactt cttacaaatg tatagcgaat gtgaagcctg ctgttttaat
     1561 tgttctagaa aaacttttga gtgaacaatt attttatgtt aggatgaatt aatttctcta
     1621 gagtttataa
//
LOCUS       DARBEG010000193         1615 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000193 DARBEG010000000
VERSION     DARBEG010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.199"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(563..1177)
                     /locus_tag="SCK20_002848"
     CDS             complement(563..1177)
                     /locus_tag="SCK20_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1146850.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          531 a          296 c          255 g          533 t
ORIGIN      
        1 tttttatttt tagattagag aaaatctcgt cctgtttttt tcattttaca atcattgaaa
       61 aattgaattt cattgcaaaa aaaataataa acacttataa atatccatta tctatccttc
      121 aatttttgtg tattttattt ctattagaaa aaggtatcct gttggacaat tttgttatat
      181 caggtaaact tcattgcaat aatgataaca aatgtactat aattactttg taaagagttt
      241 tggggaagac tctttcatca acgtgtatta agagacaaga aagaatccat atggattctt
      301 tcttgtctct ttttccacta ctttttattt ttctgacact acatattctt ataactcatt
      361 tctcataact gtgaaacaac ttataaaaat aacaaacttt aaacaaaaca agcttaaaat
      421 caaagatgat ccatcttaaa ataactaaaa caaaaaaagt agtcgttgtt aatcctcatt
      481 aataatcgtt ttcttttttt caaacgctcc atttttatgt ggaacattat ttttttaagg
      541 aactttgtca acatgtggat aactattttc cacttgtgtc ctggactagc tgttttgctg
      601 taaaatacaa aacttgctgc agcaaaaaga tcgttgcaag agaggctgtt ccgcagatcg
      661 ctggatctag atttggcaaa gtcaagactg catcatgtaa attggcgtgg ttgtccaaaa
      721 ccaaatcagc aaattcaaat aatcgcaaac cactgctatg ttgcgatttg atacttcgtg
      781 acaaatcaaa tccagtaaca atgattaatg gatgaccatt ttctttgatc cactgagcta
      841 gttcgatgat tgccgggttt cttccttcat tcgaaagaag aagaaaaatg tcttctgatt
      901 tgacagaaag ctgatcaata attgcctgtc ctgcaccttc tagcgtttca tagacacctc
      961 cggctggatt ttcaatcaat atacttggta caagatccgg ggtttgatcc caaaattcaa
     1021 atgctacacc acttagtgtg cggctagcat atatctgcaa tctaccacct tgattaattt
     1081 gtttcgcgat ccaagtgcca gcttgaagca tcagcggctc ttctatcctt aatttctctt
     1141 ccaattggtg ataatagtca aaataacata aactcattta tctctcccgc ctagctatac
     1201 ttgacatgaa tagaaacagc tgcctaatct aaggaagtac ataaaaaaac agtagttaga
     1261 cagtaacaca tgtggtcttt gctgtttcaa acacaaaaaa ggcattgaac gaatacgccc
     1321 aataccttga catagcaaat aaaacaacgt gcagaccatg gaaaatatct acataggggt
     1381 agaattttca ccctagatta ggtacaataa attaggtcga tgcactgcat tgactatggc
     1441 aaaatgatcg gtcagggtgt atgtgtgtga ctggtcattc cctgattctt agcgccaact
     1501 aagggtcagg gagccttttt ttattcagct gtttctatct gaatttattg taagtaatct
     1561 cttatccaag tgcaagcaag aaaatacaaa aaaacattgg tatgatttat atata
//
LOCUS       DARBEG010000194         1613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000194 DARBEG010000000
VERSION     DARBEG010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.200"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            523..>1613
                     /locus_tag="SCK20_002849"
     CDS             523..>1613
                     /locus_tag="SCK20_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146851.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT          545 a          159 c          284 g          625 t
ORIGIN      
        1 atcaccttcc aacagtatat aaaatgagcg taaaaacggc tatttttgcc ttaacaacga
       61 tttttttgta tacaaatacg atatgctcat taattattgg aatttgtagt gctgtttaaa
      121 ttaatgaagg tgtaattata tgagtgatca ttttgaaaaa gtttgttgag aagatgtgct
      181 tctagtcatg tgatgggaga aaataattta actgttagag tgaatagtta aattattctg
      241 gttatatgaa aaaagtagtt tctaaaataa tcttgcaggg attaacttaa agaaattgag
      301 agaatagttt ctttgaaatt tctatataca atgagaagct gaaatttact tttatttgat
      361 gactggaaat agaaattaaa tatattgtat tgttacatta aagaaaactt gtatggaaaa
      421 atcagtacaa taccattatt taatgttctt cacttaaagc gcaaaaaata tcagtttatt
      481 aaactcgtat atagaacaaa tatatgtaga cggggcgaaa cgatgagtgt aaaaaaaatg
      541 ctatttatgg taagtacaat aattgtatta acatcccgat tttggggaat taatactagt
      601 ttatctatgc gatattttat tatggcaata tgggatttat attttatgtt aagctatttt
      661 tcttatcgtg agaaaacata taaaaaatac gttatcaaga ataacttttg gttgactata
      721 atgccgtttg tgatattttc tatctatact ctaataatat ggggaattgg ttcaaatgca
      781 gaatttgaaa attttataaa gttatgttcc acgttgcttt atttaattct tgcatatggt
      841 tacgcatgta cagcatttta cttatttaag gcagaaggaa ttgattttat tttttggtca
      901 ggtgttattt catatatgag tggttcagtt ttttatttaa ttgtatctta tggtttaaat
      961 ggtttaattt cttatataaa aagtcttata acaggtgaaa caaatactgc aaattttgct
     1021 atggaggtac atgatttaac ttttgcgatg ggggtttttt tcctttatta cgtatttttt
     1081 gaaaataaaa atacaaaaaa acataagata aaaattatcg tatctttaat tttaattttc
     1141 atgggattga agagaattga actgttagct ttaagtggtg caattctagt gtattatgtt
     1201 ttattaaagt ggggaaaaaa gattcaaatc cgtgcattag tgtgtactat ttgtgcaact
     1261 gttatttcgt tagtgtttat ttacattata gatacaggaa ttttggcatt gcttatgtca
     1321 aatttgggca ttacagatga cggtgcgagg ctttcatttt atctatattc atctaaattt
     1381 tatgaacttg gtttaggata tgtaggaaca ggatggacat ggttttcaaa atactttttt
     1441 aatctttatt tatcgcactt taggttaaat ggtggaagaa ttgcagcaag cctacacagt
     1501 gatatattaa ctatttatat agaagttgga tgtatagttt ttattatatg gatgttttgg
     1561 ttattttttg tgaaaccgat aagactaact aaaaaatttg gattgaaaac agg
//
LOCUS       DARBEG010000195         1599 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000195 DARBEG010000000
VERSION     DARBEG010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.201"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..613)
                     /locus_tag="SCK20_002850"
     CDS             complement(<1..613)
                     /locus_tag="SCK20_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146852.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
     gene            901..1554
                     /locus_tag="SCK20_002851"
     CDS             901..1554
                     /locus_tag="SCK20_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1146853.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          574 a          224 c          297 g          504 t
ORIGIN      
        1 gcaggaagta ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat
       61 gggttgtccc atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga
      121 actcatccat acacaacacc gtcggaagga agttcatacg tggctggtaa tgcttcgttg
      181 cttcacgcaa gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt
      241 tacgtgattg attttcgtac agttctatga gaatcgcctg cttcagttcc tttgttagtt
      301 gatgatgttc atcaactaag tccgttttcg cagaaaaggt ctgcccacaa tcatagcata
      361 gaaaacgggt acgttttaat tctaaaatag tcaacttttc tttgactttc aatatctgag
      421 tacgtgtcgt ataggaaccg ttgcggataa tacgaatcga tccacagtgt tcacaatggg
      481 aaggtgagtc atccaatctc cctttaataa actgcgcttg aacgccttta tattcttttt
      541 caatcagcca ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag
      601 tatgattatc catagagcga ctcctttggt agggtgatgt tttggtcgac taaattttac
      661 caaaagtgag tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc
      721 gattttccaa caacacaaaa tattatagaa ccctttattc taaaggattt caaggtgtct
      781 gtttgcattt attagaaaaa agaaaaagta ttggcaacag aaggtgtcac caacactaga
      841 tattatagag ccataatatg ctgtataata tctagtgtat aggcttaagt ggaggtatat
      901 atgaaaatat taactgttga agatgataat atgataagag agggaataag tgaatatctt
      961 tcagaatttg gatatactgt tattcaagct aaagatggaa gagaagcctt gtcaaaattt
     1021 aatagcgata taaatttggt tatcttagac attcagatac cttttataaa tggtttagaa
     1081 gtgttgaaag aaattagaaa gaaaagcaat ttaccaattc taatcttgac tgcatttagt
     1141 gatgaagaat ataaaattga tgcatttact aatttagtag acggatatgt agaaaagcca
     1201 ttttcattgc cggtcttaaa agctagaata gattctttaa ttaagaagaa ttatggacat
     1261 ttagagaagt ttgaatataa ggatctatcg gttaatttta atagctatac agctaaaata
     1321 aatggtgaag aaatagatgt aaatgcaaaa gaactagaag tattaaaatg tttattggat
     1381 aataatggac aagtgttaac aagaatgcaa attattgatt atgtatggaa agatagcgaa
     1441 gaaactcctt atgatcgagt aatagatgta tatataaaag aacttagaaa aaaattacaa
     1501 ttagactgta taactactat aagaaatgta ggatataaat tggagagaaa atgaaaaaat
     1561 taaagatatt tccaaaaatg ttcatacaaa tattttctg
//
LOCUS       DARBEG010000196         1594 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000196 DARBEG010000000
VERSION     DARBEG010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.202"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(281..580)
                     /locus_tag="SCK20_002852"
     CDS             complement(281..580)
                     /locus_tag="SCK20_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1146854.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            931..1563
                     /locus_tag="SCK20_002853"
     CDS             931..1563
                     /locus_tag="SCK20_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1146855.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          549 a          246 c          213 g          586 t
ORIGIN      
        1 ttacttattg ctttcatttt ttcaatctat atttccaatt tttaacttac aaaaatgcaa
       61 cattaataaa taacgatagt ttcataaaat acacttttaa ttaacattat tagtttatac
      121 tagctgtttt tgtgttaatt aaaggataaa atacaatata aagaaacatc tgtttcttta
      181 tttagaataa aaaaatacag actctgttca aataaatgaa cctagcctgc attatgagat
      241 tgactatact ttttaattca ttgagatgaa taactcgttt ttataaatgc ccaaaagtaa
      301 tcggtgccga agccattcct cgacctaatt ctttttcaaa ttttcctatt gttgatatct
      361 ccatataaaa cggtgatact ggtttagaca attctcccct gattgctaac ggacgtaaac
      421 tacgttccaa attatttatg actttaggat aatactgacc tttttctaag cctgtctttg
      481 cgtctatcaa aagtatacgt tccttttctt tgatctctgg atttaaaacg agatcatata
      541 attttagtac catgttttct atttcttcac ttttagacat cacgatttcc tcccaaaaat
      601 atatttcttc ccactacatt ctataaacaa agtatacgct acatctagat ttttttaaat
      661 cattactgga ttgttaaaac ctttcaatac actgttatgg caacgatttt tattttaaaa
      721 acattctaaa ttaaatcagt aaaaattttt attctaagat ttttattaga attacgccta
      781 atttgtgtct ctcagggcaa ttctaagaac gtttcaagag atagcgacac tatcgttatc
      841 gaaaaaggcg aaaacctatc tcattttctg ctaatgtaag ttggtttagc ttttgttaat
      901 atgactttat gaaaatggag gtaattaaat atgatttttc ctaaagttaa tgaagcaagc
      961 ttgaataaag gatccttttt tcatatctat gaaaacgaag attttgaact gtataatatc
     1021 ctttcacaaa aagaggtcat cgaaaaccaa ataatctgcc atccaattat ttacaaatct
     1081 attttcaagt atgcattttt tgcttccaat gattttcaaa gaatggagat taccgactgt
     1141 atattcgaaa actgtgattt ttcaggctgt atattgacaa aaagcatgtt gcatcgcgtg
     1201 aaatttgtca attgtaaatt aacaggtact aaattgatgg aaagttatat tgggaacacg
     1261 ttattcgaga attgtaaaat ggactatgta aatttatctg gttcaaacat aaaagaaagt
     1321 acctttgagc actctgtctt atcaagcgca gattttgtag actgttccct cactaaaata
     1381 atgttttcta ctaatgattt agaaaatgct agcttttttg atactgattt aaaaaatatc
     1441 gatttaagta gcaactcctt tgaaatgatt gaagtacgct cggaacatct aaaaaattgt
     1501 acgcttaatc agttgcaagc acttggtttt gctagaaaat ttttacaaat aaaagtagta
     1561 taatcaaaat gaatatataa gataaatttt tagc
//
LOCUS       DARBEG010000197         1519 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000197 DARBEG010000000
VERSION     DARBEG010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.204"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(261..1184)
                     /locus_tag="SCK20_002854"
     CDS             complement(261..1184)
                     /locus_tag="SCK20_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146856.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT          503 a          271 c          149 g          596 t
ORIGIN      
        1 tctcaaatta acataagaaa tccccagtgt atttaacagt tgtaaatgct tctgaattga
       61 agtgtgattc attttcatta attaccactc actttctaaa aaatttttat ttgtatgact
      121 acattacctc caacctctca taatttcatg atcttcagtt ctacaaaaat atatcccact
      181 caaaatcttg acaaaagaaa aaagttgtga ttttggaaat cacaactttt ttcttttacg
      241 ataaatcaat ccttattaaa ttatttagga aaatattgtt caattatttc tttatatatt
      301 ttctcatagt atgtttctgt ccatcctaat gttcctataa aatcaagtcc tctacatttt
      361 aaaggttcac ttaaactatt aatatagtta ataatgttgt ctatcctttt ctctttatct
      421 tcaccttcaa tctggggagc atatagtcca atttcctccc aataatatga atctctttca
      481 attcccatga tggtagcttt ggctaatttc aaatcaaata acgattttag aattcttagt
      541 gtctcaataa agtcttctct cttcaactct ttaatccatt gtgaattttc tggattatct
      601 aaaatttcaa gttcatcttc attataagta tatttcaaat ttgacttgct taactcatag
      661 ataaatgaag tttcttgact cataattctt ttaagtactt gactataagt ttcgtttgac
      721 tttgcccatt tcgctaattc taccactgta ttcactaata aatcgaaata atgatgttct
      781 cgttcaatag tctcagcatt ttgaaaatga aaatcacctt tataaactcc attcttttcc
      841 actgttttat taatattttt taaaaaccag aatgagtttg caaatttagt cgtacttttt
      901 cgattaacta agttaataac tcttccatgt aataataaga caaaaataca taaattttta
      961 gcttccttat tattagatac taattgatca atcataggga gccatttttt gattttcact
     1021 aattgattgc tcataaatct ttcactggac ttttgttcaa tcttaaattg caaaacttct
     1081 aactcataat tcccatcttc tgatttattt ttgggaacaa ctatcaaact tttataaagt
     1141 ttatccacat caaaatttat cttttttgca taatttgtag ccattaatac atctccttat
     1201 tcataataaa tttcatatga cgctcctctc aaaatttacc ggtagactag aaaaaagaat
     1261 aaaagatacg tcaattcaca ctttattctt aaaaaaaaac tggtctctct accggtaata
     1321 tcgattgagt ccgcctttat cgactttatc ctttttttat attaaatttt ttcaccggta
     1381 gacagggaga gaaagaataa actaaaaagc ccttaattct taatgttttc acctccttac
     1441 cgttcctacc gctaaaattt aatataacta aattctataa cattagaaga aattttaaat
     1501 acctttgata aaaaatatt
//
LOCUS       DARBEG010000198         1518 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000198 DARBEG010000000
VERSION     DARBEG010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.205"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..221
                     /locus_tag="SCK20_002855"
     CDS             <1..221
                     /locus_tag="SCK20_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG1146857.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="SCK20_002856"
     CDS             218..1237
                     /locus_tag="SCK20_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG1146858.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1426..1499
                     /locus_tag="SCK20_002857"
     tRNA            1426..1499
                     /locus_tag="SCK20_002857"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Ile,seq:cat)
BASE COUNT          495 a          286 c          316 g          421 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1501 taacgtagcc atttgaat
//
LOCUS       DARBEG010000199         1478 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000199 DARBEG010000000
VERSION     DARBEG010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.207"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            290..697
                     /locus_tag="SCK20_002858"
     CDS             290..697
                     /locus_tag="SCK20_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146859.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            711..1112
                     /locus_tag="SCK20_002859"
     CDS             711..1112
                     /locus_tag="SCK20_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146860.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1114..>1478
                     /locus_tag="SCK20_002860"
     CDS             1114..>1478
                     /locus_tag="SCK20_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146861.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKL"
BASE COUNT          566 a          180 c          258 g          474 t
ORIGIN      
        1 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
       61 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      121 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      181 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      241 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      301 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      361 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      421 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      481 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      541 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      601 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      661 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      721 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      781 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      841 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
      901 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
      961 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1021 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1081 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1141 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1201 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1261 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1321 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1381 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1441 ctgatgaatc gagaagcaga aaaaataata aacaaact
//
LOCUS       DARBEG010000200         1476 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000200 DARBEG010000000
VERSION     DARBEG010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.208"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            27..1217
                     /locus_tag="SCK20_002861"
     CDS             27..1217
                     /locus_tag="SCK20_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEG1146862.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            1351..>1476
                     /locus_tag="SCK20_002862"
     CDS             1351..>1476
                     /locus_tag="SCK20_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1146863.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTA"
BASE COUNT          468 a          291 c          308 g          409 t
ORIGIN      
        1 acattcacag aagagaggac ttccctatga acgattttac tacagaaatt ctaaagactc
       61 tagcgaacaa aggcgatttg aatgaattat tccgtgtcca tttggagaaa gcagtcaata
      121 cgcttctcaa aacggagtta acggctttcc tagattacga aaagtatgat cgcattggtt
      181 ttaacacggg taattctcgt aacggctcct atgaccgtac ggtcaagacc gagtatgggg
      241 aacttcatct ccagattccg cgcgaccgca atggtgagtt caagcaacag actgttcctg
      301 cttatagacg gacgaatgac acgttagagg agaccgtcat tcacctcttc cgaaaaggta
      361 ttaccatgtc ggaaatcgca gacttgattg agaaaatgta tgggcattac tacacgcccc
      421 aaaccatgtc caatataaca aaatcattta cagaagaggt aacggcgttt aatgggcggg
      481 agcttcatga ccgttatgct gctatttata tggacgcaac gtatattccg ttaaagcgga
      541 aaaccgtcgc caaggaagct attcatatcg cagttggcat tcgcccggac ggatcaaagg
      601 aagtattgag ctatgcgatt gcaccgactg aatccatcac gatttgggag gaaattttat
      661 tggaccttca agagcgcggt ttgaaaaatg tcctcctgtt catcacggat ggcttaaagg
      721 ggatggtagg agcgattagt cggttctatc ccaaagctcg ttttcaacat tgttgtgtac
      781 acgtttcccg taatatcagt cataaagtgc gtgtcgatga tcgtaaggaa gtctgtgatg
      841 attttaaaat ggtgtatcaa gcctcatcta aagaggtggc gttggaagca cgtggtactt
      901 ttgcggaaaa atggaaaacc agctatccaa aagtggttga atcgattctt tcgaacgatc
      961 acttgctcac tttctatgat ttccccttgg ccatacgcaa gagtatttat tctacgaact
     1021 tgattgaatc ctttaataaa caaatcaaga aatacagcca ccgcaaggaa cagttccaaa
     1081 atgaagagtc gatggaacgt ttcttagtct cgtcttttga tacttacaac caaaaattcc
     1141 taggtcgcag tcataaaggt ttccaacagg ccgaaggcga gctcgaacaa atgctaagcc
     1201 aactgattga gaattagaaa caatctgcag tagggaagaa tcatttacac aaaattattg
     1261 acgctccctt ataatgagaa cttttactaa cataatttat gataaactta ctatataatc
     1321 atattaatta aatagttagg agatctgctt atgtttatat ctactcttca aattcgaaat
     1381 tttagaaatt atgaatccga aatctttcga ttcgataatg agacaaatac tattataggt
     1441 gagaatgatt cagggaaaac caatgctctt actgca
//
LOCUS       DARBEG010000201         1454 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000201 DARBEG010000000
VERSION     DARBEG010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.209"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SCK20_002863"
     CDS             complement(<1..625)
                     /locus_tag="SCK20_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1146864.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SCK20_002864"
     CDS             complement(784..1050)
                     /locus_tag="SCK20_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1146865.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SCK20_002865"
     CDS             complement(1040..1396)
                     /locus_tag="SCK20_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1146866.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          588 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aatt
//
LOCUS       DARBEG010000202         1453 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000202 DARBEG010000000
VERSION     DARBEG010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.210"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..337)
                     /locus_tag="SCK20_002866"
     CDS             complement(<1..337)
                     /locus_tag="SCK20_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146867.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKN"
     gene            complement(339..1397)
                     /locus_tag="SCK20_002867"
     CDS             complement(339..1397)
                     /locus_tag="SCK20_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG1146868.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          560 a          237 c          177 g          479 t
ORIGIN      
        1 gatttttttt gtaaacattg gagaaaagta tttttgtgaa ataattttcc ccctgtataa
       61 aaatttcatc ataaacggat aaatcatccg agaaaaattg attatggtta gttaacaagt
      121 taattttacg tttaaaggaa gaaccgattt tccggtgatt cacaaaaaca acatcattaa
      181 atagccctaa ttcttttata cgctcactat atcttctagc gttttcaaat tcagaataaa
      241 taaataaatc tccttttgat tctcggtaaa aattttgata aacatttaat atattaatca
      301 aatgtaaagg agtccaagca atgaaagctg tcttcatatt aattctcctt ttttaaatac
      361 aagtaagaaa tgcttcccaa ataataccag aaaacaattg aaatgacatc gcgcttattg
      421 aaaagtacat gagcctccac catttcatct ataaaaatag atagtataaa aattaaaaca
      481 ataccaagaa acgcataatt cactttactt ctaaaaaatg ttttgagtaa actaaagata
      541 tttaacacat agaaagcata gatacatata aaagcaatta taccacttga tacaagtact
      601 tgaagaaatc cattatgcat gtttccatgt gctcttttta gttcattcac atctgtagta
      661 tcaagatcta cttttttcaa aaattttgca tcgctattcc catagaaatc ggtctctcca
      721 taaccaaata acaaagttgg tcttataact ttaagcgcag ctttccaaat cgtaaaacga
      781 ccagctgaaa tttcggagtt atcactagaa tgctgtaata ttacttcttt ttctggaagt
      841 ggagttttat ctgattcatt tgactctata acttctactt ttctatcaat cgatacagga
      901 attgttgcta aaaaatcctt agcataatta gtaccaaaag aaaaatatac caatagaact
      961 aataatatgc ttaccatcca tttgattttt acaggtagta cttctctaat gctctttcta
     1021 aattttgaat tggcaaacaa caaaagaaat aatactaaaa aagaacaaat tactacctgg
     1081 gcacctctag acgaagaaag tgtaatataa aatatttgta cgataaaatt catcatataa
     1141 aaaacacgaa tcgtttttgt tatttttgaa attaatacac taattaaact taaaataata
     1201 gaagtatatc caaacatagc tccaacattt ggacttgtat agacaccaaa taaccgatgt
     1261 tcaataaaac cttgtcttgc aagtaatcct ggcgttcctg ttgggacatg ataagaaatc
     1321 aaaaaaataa attgatagat agatattagc gcagctaaaa aaattatgat aatgaaaaca
     1381 ttattaaaat acaccatttt tttctttttt tcctcttcac tttgtgaaaa gtctgcagga
     1441 aaaattacta tca
//
LOCUS       DARBEG010000203         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000203 DARBEG010000000
VERSION     DARBEG010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.211"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SCK20_002868"
     CDS             105..1406
                     /locus_tag="SCK20_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1146869.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARBEG010000204         1417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000204 DARBEG010000000
VERSION     DARBEG010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.212"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SCK20_002869"
     CDS             complement(150..1337)
                     /locus_tag="SCK20_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG1146870.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DARBEG010000205         1410 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000205 DARBEG010000000
VERSION     DARBEG010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.213"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            52..1221
                     /locus_tag="SCK20_002870"
     CDS             52..1221
                     /locus_tag="SCK20_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1146871.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          503 a          256 c          230 g          421 t
ORIGIN      
        1 attttagctt tattctttct gtaaaaaaga aaggagacta aataaactat tatgaaaaaa
       61 ggaaaacatg ctaaaaaaag aaaatattca tttttaatga tcgttgtcgt gttgtttagc
      121 cttagtgcgt tctctacagg aactattctt ggatggcaat tttacaataa tgaacactct
      181 cagatagttg cccatgctca atcagctcaa aaaaagttga tactgactac caatgaacaa
      241 cagctcgttg aaaaaatcga tcaactatta agaaaaaatg attatattgg ctcaatttac
      301 gtaaagaaaa ataatcgcat catcatggaa aaaggctatg gctatgcgaa taaactcacg
      361 caacaaccaa atgatccttc tctttattat cagattggtt cggttcaaaa agcattgact
      421 actcttttga ttttaaaaca agtcgagcgt gggcaccttt ctttagatac taagctggct
      481 caattctatc cacaaattgc tggcagccag caaattacta tccgtgactt gctgtatatg
      541 cgctcaggct tgcaccgaac tgcttctcca accataccga tgactgatga agaagtcgtt
      601 caatttgcga ttcagcactt aaaatttaca gattatcaga cctctcatta tgattcattg
      661 aactttacca tattgactgg aattttgatc aaattaacaa atcaaccgta tgaaaaatta
      721 ttgaccgatg atatcatcga acctttagga ttacagcata caaaatttta tgaccaagtc
      781 aaaaattcat ccaatcatgc actatcctat caaatgtcta aagaggatga ttactttaaa
      841 ccactgacag aatctgaaac agagatccat aacgaattag ggactggtaa tatcagtatg
      901 tccgtctatg atttaaatac gctgtttaca caagttttat ctggaaaact agtttccaaa
      961 gacttactct tctcattatg gcgtgaaaat gcgaatggac atccatatag tggtggggta
     1021 tatagcggag ataattatat cctgtcgcaa ggaaatatcg actcttttga tacatctgct
     1081 atatttagca aagattcaca ggatgctatc atcatggaaa gcaatataaa agctgacaaa
     1141 actatcaaac tgcctactgc tgatctaaga aaccaaatct atgacctgat ggaaggaact
     1201 actaagttgg caaatagctg atatgatgaa aagatagcaa atactacttc tttgaatagc
     1261 atttgcttaa aaaaagtact cgactaaacg taggaaagag tgtgggacaa atctaaaaat
     1321 caatttttgc tacagacttt aattccaaat aaacagaaca gaaagaggtg ttcttctttc
     1381 tgttctgtcc aaattattct aagatttctc
//
LOCUS       DARBEG010000206         1364 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000206 DARBEG010000000
VERSION     DARBEG010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.214"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(140..1288)
                     /locus_tag="SCK20_002871"
     CDS             complement(140..1288)
                     /locus_tag="SCK20_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146872.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          392 a          242 c          217 g          513 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcggatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgcc
//
LOCUS       DARBEG010000207         1355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000207 DARBEG010000000
VERSION     DARBEG010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.215"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SCK20_002872"
     rRNA            <1..1355
                     /locus_tag="SCK20_002872"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARBEG010000208         1337 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000208 DARBEG010000000
VERSION     DARBEG010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.217"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..280)
                     /locus_tag="SCK20_002873"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="SCK20_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="SCK20_002874"
     CDS             complement(546..740)
                     /locus_tag="SCK20_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146873.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="SCK20_002875"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="SCK20_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          478 a          218 c          180 g          461 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagat
//
LOCUS       DARBEG010000209         1299 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000209 DARBEG010000000
VERSION     DARBEG010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.218"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1178
                     /locus_tag="SCK20_002876"
     CDS             <1..1178
                     /locus_tag="SCK20_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1146874.1"
                     /translation="EDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRF
                     SQEYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAG
                     YIGVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKK
                     YLGRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNI
                     KDIYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSI
                     FNYNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGV
                     QLCSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          520 a          152 c          210 g          417 t
ORIGIN      
        1 tagaagatct tttttatcaa aatagaatga cactaaattc tgagataaaa aagttatttc
       61 tagagacaga agttgacaaa attgatattc aaaataaaaa gattagttgt gtaaaaaaag
      121 atggtacaag attctctcaa gaatatgaca aagttatatt agctacaggt tcaaagcaaa
      181 ttatgctaga tataccgggg aataatttag ctaatatttt ttcgataaag aattttccaa
      241 atgcaaaaga gatttttaaa gctttggatg atagtactat taaaaaagta ggaattattg
      301 gagcaggata tattggagtt gaaattgctg aagccataag aaaaagaggg aaagaagtat
      361 atcttttcga tgttgcagat agagtgttgt cgacttatta cgaccgtagt ttttcagata
      421 aagttgaaga aatactatca aaaaatggaa tacatctttg tctatcagaa agtcctaaaa
      481 aatatttagg aagaaaaaag attgaaaaaa tagtcactac aagtggtacc caatactcta
      541 tggatatggt cgtacaagct ataggatttc tatctaatag tccaattggg gaaaaggaac
      601 tcttgagaga tgttagtgga gcatatctaa ctaatgaata ccagcaaaca aatataaaag
      661 atatatatgc tattggggat tgtgctacaa ctttttttac gtccattaat agaagtagtg
      721 ttgatttttc tatttctaat gctctaagaa cagcttatat tgcaactcaa catattaaca
      781 atcaagattt tacaccttct ggttcccaat ttacaaatgg cttttctata tttaattaca
      841 atttttacgc agcaggtttg aatttaaaaa cagcacgatt attaagtgag tctatagatt
      901 atatagaaat agaagatttc attagacctg cttttttgag aaaaaatagt cgtgtaaaat
      961 taagaattag ctttgaaaaa aatacaaaga gaatagtagg tgtacaactt tgtagtcaag
     1021 aagatttatc tggactatta tcaatgtttt ctcttgctat agaagaacaa attacattag
     1081 acaaattgaa aatgttagat tatttatttc atcctagttt tagccaaccc tataatttta
     1141 ttttaaaggc agtaacagga ttaaacattg aaacataatc gttactaaat gaaatcaaga
     1201 aatatactgt attactcaat tcattgaaaa tatttatcat aatttttaat agaaaacact
     1261 attaaagaga aaaaagagag gtaaatcaaa tgaaaaaat
//
LOCUS       DARBEG010000210         1294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000210 DARBEG010000000
VERSION     DARBEG010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.219"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1244
                     /locus_tag="SCK20_002877"
     CDS             <1..1244
                     /locus_tag="SCK20_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG1146875.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DARBEG010000211         1284 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000211 DARBEG010000000
VERSION     DARBEG010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.220"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            40..>1284
                     /locus_tag="SCK20_002878"
     CDS             40..>1284
                     /locus_tag="SCK20_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG1146876.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFE"
BASE COUNT          433 a          254 c          248 g          349 t
ORIGIN      
        1 atcaccacaa aataaacaac tgagaggacg atacgctata tgaatgattc tatcaaaaaa
       61 atgctgagaa taacagagaa agatttgatg attacagagg tctcttacga gacccttcag
      121 aagaaaaaga cgttggtcgt cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt
      181 tgtggttcta ctgtggtaga tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag
      241 gaaacgattg tccgttttga acaatacaat catatgcctt tggttatgcg cctaaaaaag
      301 caacgctata cctgtaaaaa ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa
      361 cccagacatt caatcgcaaa tcatgttaga tataaaattg cttctttact gactgaaaaa
      421 gtatctttat cttttattgc gaaaagctgt caggtatctt tgaccaccgt tattcgtaca
      481 ttgaaagagt ttaaaagcta tttaccaaag caatctaaga agatcctccc cagagtattg
      541 atggttgacg aatttcgttc gcatgcttcc atagaagata agatgagctt tatttgcgcg
      601 gatggcgaaa caggaaaatt aatagatgtt ttgcctacgc gtaaattacc tcgattaaca
      661 agctatttct taggttgtac caatccagaa gaagtagaat tcttggtgac agacatgaac
      721 gccgcctact tccagctcac caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg
      781 tttcatattg tcaaacacat gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat
      841 gaacttcgta aagccggaca gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt
      901 ttgctaaaaa atcgtgcaaa catcaaccat tatgaataca aaacatggaa aagtttccga
      961 gcaccaaaat acccatttct tactgaagca atgatgattg atcgattgct tgagttttct
     1021 ccgcccctaa aggaggcgta tccctttttt catgaattag ttgaagcctt tcgagacaaa
     1081 gaccctgact tatttttctc cttattggca gaacttcccg aaacgttaga tgacagcttt
     1141 cgggaaaagc ttcaaaacct tctgacctat gaagaaggca tcaccaacgc aatgatctat
     1201 ccttattcca atggaaaaat agaagcgaag aatacccaca taaagacaat gaaacgagta
     1261 tcctacggat ttaaatcatt tgag
//
LOCUS       DARBEG010000212         1275 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000212 DARBEG010000000
VERSION     DARBEG010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.221"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            46..>1275
                     /locus_tag="SCK20_002879"
     CDS             46..>1275
                     /locus_tag="SCK20_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1146877.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT          490 a          167 c          215 g          403 t
ORIGIN      
        1 tcagatagaa aattacacaa aaatacttta tgctgaggtt tcaaaatgac aaatatcttc
       61 gaaaaaatcg taaatgattt aaatttagaa caaaaagatg ttgtggaaag tgaagataac
      121 ctttatgtaa atgcatgtcc tgggagtggg aaaacaaggg tacttacaag gaaaattgct
      181 taccaagcaa ttaaacacga tggatcacta aaaagaataa ttgctatcac atacactaat
      241 agagctgctg aagaaattaa ggaacgccta agtttactta gtatagatga tgatattaat
      301 atttgggtgg ggacaataca tcagttttgt cttgaattta ttatttatcc ttttaaaatg
      361 aatctctcaa gagtatcaaa aggatttaca attattgatg attatactca aagagcttac
      421 gtcagccaaa ttaataatat gctgaattta aatctcaaaa cgtatgaact gaacaaaatt
      481 gatttgtcac tttctcatga ttttaaaatt aatgaagttc agtatactca agtttctgaa
      541 atgtatcaca ctatgttgga aaaaaataaa gaaattgatt ttgatatgat tttactagta
      601 gcgtataaaa ttttaactgt aaacagtaac gttgcacgta atattgctag taatataagg
      661 gcgatttatg tggatgaatt tcaagatact agagaattac aatacaatgt aatagctaaa
      721 ttacttcaaa acaaccctca aattcagtca atgtttgttg gagatattga ccaagcaatt
      781 tatggatcat tagatggtat agctaaatca gttgaagaac tagaaatgct aactagtcac
      841 acttttcaat caaagacgct acatggctgt tacaggtcta atcagagatt agtagacttc
      901 tactctaatt ttcagagttg tccttacgag attgaaagca gagggaataa taaaaacgac
      961 cgtggtttta taagttatga ttgcaaagtt actaaggagg atttacccaa cataataaaa
     1021 aatataataa aggaaaagat tgaaagcggc attcatgaaa aagatatttg tgtacttgct
     1081 cctcaacact atcctctgtt ttcactgggt gagtatttaa agaaagaatt accgtattgc
     1141 aattttgatg ctataaatat atcaccaatt aaggtaaata atcatagttt atttttcaag
     1201 ttagcaattt tgtactttac tgaatcaggt gaaaaggttc gaagaagaaa gctaattgca
     1261 aacgatattt taatt
//
LOCUS       DARBEG010000213         1263 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000213 DARBEG010000000
VERSION     DARBEG010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.222"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..1097
                     /locus_tag="SCK20_002880"
     CDS             18..1097
                     /locus_tag="SCK20_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1146878.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          375 a          301 c          310 g          277 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaccc atgataacac agtttcacgc aagggaaaac
       61 atctttccta ctcagaacgt tgccaaatcg ctatcctgaa aaaggaaggc tattccaacc
      121 gtcaggttgc caaagtgctg ggacgtgttc cgcaaaccat aaacaatgag attgatcgcg
      181 gaaccatcac ccagttaaaa cgccaaacgc aaaatggaaa agtgtatgac acctactatt
      241 ctgtttatga cgctgatgca ggacaggcct tctatgaaaa gcaacgactg aactgtggca
      301 gacgccccaa atgggcgaac acaaatgcct tcattgattg ggcagatgac aaaatgttaa
      361 atgaaaaatg gtcgccggac gtcgtggtcg gatttgccag aaaacacgag ctgttcgatt
      421 gtgccatcat tcctggcaca accgctcttt atcaatggat tgaccgcgga atcatgcgaa
      481 caaagaacat ggacttgctt gaaaaactgt cccgtaaaat caaagcggtc cgcccaggac
      541 accgtcccaa caagcgtgtt ctcgggacgt caattgaaga acgccctaaa gaagttgaaa
      601 cacgggaaac gttcggtcat tgggaaatcg acacggtagt gggaaacaag accaagtcgg
      661 atgccgtgct gctgacgctg gctgaacggc agacacgctt tgaagtcatc ctaaagttga
      721 acgggaagga caccgaatcg gtggaccaag cggttcaaca gctaagagaa cgggctggcg
      781 ctaacttcga tcaattattt aagaccgtta catccgacaa tggctcggag ttttctgggc
      841 tacacgatgc tctgcaagat gtagctgacg tctatttcag ccacccctat gcttcttggg
      901 agcgtgggac gagcgaaaac cagcacaaat taattcgccg tttcgtcccc aagggaagcc
      961 cgattagtga ggtgagcgag aaccagattt tgcggatcca gcgttggatg aacgattacc
     1021 cgcggaaaat tttgggttat gcgacaccac atgatacatt cgtccaggca ttcaagcaag
     1081 aacggttggt ggcttgaccc attattcctt gataacgatc ctcactcggg acatggcttc
     1141 atgtctggaa agctggcctg gggccagaat cgtctgaaga ctatcatacc tcagttcatt
     1201 atcaaggcct cactgattta gaataacgag gcagttacgg tatcaaacaa atgagttacc
     1261 tgt
//
LOCUS       DARBEG010000214         1259 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000214 DARBEG010000000
VERSION     DARBEG010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.223"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..1097
                     /locus_tag="SCK20_002881"
     CDS             18..1097
                     /locus_tag="SCK20_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1146879.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          332 a          342 c          356 g          229 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaacc atgataacac agtttcacgc aagggaaaac
       61 acctaaccta ctcagaacgt tgccaaatcg ccatcctgaa aaaggaaaac tattccaacc
      121 gtcaggttgc ggatgtcctg ggctgcgtgc cgcagaccat aaacaacgaa atcaaacgcg
      181 ggaccgttac tcagcttaac cgccagaatc agaacgggaa ggtctacgac tattatcatg
      241 acgtctacga cgccgatgcc gggcaggcgg cctacgagaa gaaccgcttg aactgcggca
      301 gacggccctt atgggccgac acggacgcct tcattgaatg ggcggacgac atgatgctgt
      361 tggaaaaatg gtccccggac gtggtggtgg gctttgccag aaaacacgaa ttgttcgacc
      421 cggccattat ccctgggacc accaccctct atcagtggat cgaccgtggc atcatgaaaa
      481 cgaagaatat ggacctgcta gaaaaactgt cccgcaaacc gaaatcgtcc cgtcatggca
      541 accgcccgaa taaacgggtg ctagggaaat ccattgaaga gcgcccggag gcggttggta
      601 cccggaaaac cttcggccat tgggaagtcg acacggtcgt aggaaacaag accaagtcgg
      661 atgccgtgct gctgacgttg gtggaacggc agacacgctt cgaggtcatt ctgaagctgg
      721 ccgggaagga tgccgaatcg gtcaaccaag cggtccgaca gctgagagaa cgggctggtg
      781 cgtacttcgg ccagctgttc aagacggtta cctcggacaa cggctcggag ttcgcgggac
      841 tgcacgatgc cttgcaggat gtggcaaaca tctacttcag ccacccctat gcttcctggg
      901 agcgcgggac gagcgaaaac caacacaagc tgattcgccg tttcatcccc aagggcagcc
      961 caatcggcaa cgtgagcgaa aaccaaatct tgcggattca gcgttggatg aacgattacc
     1021 cgcgaaagat tttgggctat gcaacgccgc atgacacgtt cgtccaggca tttaggcagg
     1081 aacggttgac ggcttgacac gtgggccctt gacaacgagc ctccctcggc atggcttgtg
     1141 tctggaaagc tggccggggc cagccagtcg ggagactacc atgcctcagc tcattgtcaa
     1201 gggcccactc gattagaaaa attagacggt tggtggtaac gagcaaccgg gtcaccatg
//
LOCUS       DARBEG010000215         1246 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000215 DARBEG010000000
VERSION     DARBEG010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.224"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            200..742
                     /locus_tag="SCK20_002882"
     CDS             200..742
                     /locus_tag="SCK20_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146880.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="SCK20_002883"
     CDS             898..>1246
                     /locus_tag="SCK20_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG1146881.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DARBEG010000216         1215 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000216 DARBEG010000000
VERSION     DARBEG010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.225"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(305..661)
                     /locus_tag="SCK20_002884"
     CDS             complement(305..661)
                     /locus_tag="SCK20_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG1146882.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /gene="spx"
                     /locus_tag="SCK20_002885"
     CDS             complement(675..1085)
                     /gene="spx"
                     /locus_tag="SCK20_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1146883.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          391 a          223 c          170 g          431 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttct
//
LOCUS       DARBEG010000217         1215 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000217 DARBEG010000000
VERSION     DARBEG010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.226"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            414..947
                     /locus_tag="SCK20_002886"
     CDS             414..947
                     /locus_tag="SCK20_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG1146884.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DARBEG010000218         1207 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000218 DARBEG010000000
VERSION     DARBEG010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.227"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            79..951
                     /locus_tag="SCK20_002887"
     CDS             79..951
                     /locus_tag="SCK20_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1146885.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          365 a          226 c          280 g          336 t
ORIGIN      
        1 taaataaaaa aataatttaa atatgatata tagtcattta tttagtgatt tcattctaaa
       61 ataaagggag aaatgtctat gctatacggt gcaattgaag caggcggcac aaaatttgtg
      121 tgtgccattg gcgatgaaga aatgacgatc aaggaaagag tgagctttcc gacaacgaca
      181 cctgaggaaa caatgccttt agtcattgac ttcttcaaac aataccaagc agatttagca
      241 gggattggga ttggctcatt tgggcctatc gatatccatc gcgattccgc cacttatggg
      301 tacattacct cgaccccgaa attggcatgg caaaattttg atttcatcgg taccatgaaa
      361 aaagaatttc cgatcccgat ttcttggaca acagatgtca atgcggctgc ctatggtgag
      421 tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt actatacgat cggtacgggt
      481 gttggcggcg gtgccttaca agaagggcgc tttatcgaag ggtttagtca tccagaaatg
      541 gggcatatgc tcgtcgtccc tcatccaaaa gatgactttg ctggcagctg tccatttcat
      601 ggcaactgtt tagaagggat ggccgctgga cccgccatcg aaaaacgctt agggaaaaaa
      661 ggacaagagg ttgcagaaga tgatccttat tgggaaatcg aagcctccta tattgctcaa
      721 tgtgcctaca atacaacctt gatgttttct ccagatgtga tcatttttgg cggtggtgtg
      781 atgaaacaac gccatatgct gcaaaacgtc cacgacgcat ttgcaaaatt ggtcaatggg
      841 tatgtcgaaa cacctgagtt aagcaaatat atcgtgaccc cagcattaga agacaacgct
      901 ggaacgctcg gttgtttggc attggctcga gaagcggtct tacattcata aacgaaaaaa
      961 ggctcttcga catttagtgt tggtagagaa agatacaact gaaaatttag tcataaccag
     1021 ttaggcagag tgcttaactg gtttttgttt tacttggaag atcaagcctt gaatgagata
     1081 aattcttgct cgaaagttat ggaaatttcg atagccgtag gccacttgta ttgtgcaata
     1141 gttttttaca cgccgtttgt ataattaagc gagacaaata aaaaagccat ttatgttaca
     1201 ctcaaat
//
LOCUS       DARBEG010000219         1207 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000219 DARBEG010000000
VERSION     DARBEG010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.228"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            366..449
                     /locus_tag="SCK20_002888"
     tRNA            366..449
                     /locus_tag="SCK20_002888"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:400..402,aa:Leu,seq:cag)
     gene            complement(773..>1207)
                     /locus_tag="SCK20_002889"
     CDS             complement(773..>1207)
                     /locus_tag="SCK20_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1146886.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          407 a          195 c          261 g          344 t
ORIGIN      
        1 tcgttactta tgatctctag aagaaagaaa acgtgaatta cttcagcgtc tagaattcca
       61 gaaggatagt aatcaacaga agaaataatc aatatccaaa tgaaagaaga tagctgaatc
      121 gagaaaaaga ggaaaaaaga aatgaagaaa aaaataaaat agttactaat cccagagtcg
      181 ttcgtttaac attgaaatcg atcatatgga taaaacagat tttttcacca accctcaata
      241 gatgatggag ttgctcggat gaattatttg tgtagatggc ataagggagg gcgtacagaa
      301 atgaaaaaga acaaaaatat gttcttgaca aaaaacacat gtaatcctaa actaaaagat
      361 gtgatgcggc tatggcggaa ttggcagacg cgctagcttc aggcgctagt gagggtgacc
      421 tcgtggaagt tcgagtcttc ttagccgcat aatttgtttt tttataaaga gaaggtacac
      481 ctggagcgat gagaaagaaa ataacatatt atctaaaaga atataggaaa aattttaaac
      541 aacggaacca gattcaaata aatcaaaaaa aatttgtgtg aaggtgggac agtttaaaac
      601 ctaatagatt gattcaaata gtagatttat agattctgat acaagaaata ctggcaaacg
      661 atagttaagg aataaagact aaacttaaac tcaaaaagag gtcatgcgaa agaataaatc
      721 gcatgacctc ttactatatt ttgaatgtgt ttaaacgttc aaataatgaa tcttagtacc
      781 aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt gaagtaacat
      841 attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag taagaagaat
      901 ctaattggta acggccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt
      961 tttgtgcgat ccattctttt gcagagcttg tacttgctgg agtagcagca gtttctgtta
     1021 cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tgtttccgtt tgaaccgttt
     1081 cttgtacaac aggttgttca gcttgaacag gttcagcttg gactggtgct tcttgaacgg
     1141 ctgcttgagg ttcagcagct gcttgtgcag agccttctgt tgggattgtt aattgttgtc
     1201 ctgagta
//
LOCUS       DARBEG010000220         1193 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000220 DARBEG010000000
VERSION     DARBEG010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.229"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1076
                     /locus_tag="SCK20_002890"
     CDS             <1..1076
                     /locus_tag="SCK20_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1146887.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DARBEG010000221         1183 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000221 DARBEG010000000
VERSION     DARBEG010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.230"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SCK20_002891"
     CDS             <1..153
                     /locus_tag="SCK20_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1146888.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="SCK20_002892"
     CDS             159..575
                     /locus_tag="SCK20_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146889.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="SCK20_002893"
     CDS             681..944
                     /locus_tag="SCK20_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1146890.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1183
                     /locus_tag="SCK20_002894"
     CDS             944..>1183
                     /locus_tag="SCK20_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1146891.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVLLRSLKIKR"
BASE COUNT          448 a          154 c          239 g          342 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggttctgt tgcgaagttt gaaaatcaaa cgc
//
LOCUS       DARBEG010000222         1171 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000222 DARBEG010000000
VERSION     DARBEG010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.231"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..156
                     /locus_tag="SCK20_002895"
     CDS             <1..156
                     /locus_tag="SCK20_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG1146892.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>1171
                     /locus_tag="SCK20_002896"
     CDS             175..>1171
                     /locus_tag="SCK20_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1146893.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIM"
BASE COUNT          497 a           95 c          205 g          374 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg c
//
LOCUS       DARBEG010000223         1160 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000223 DARBEG010000000
VERSION     DARBEG010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.232"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SCK20_002897"
     CDS             <1..704
                     /locus_tag="SCK20_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1146894.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
     gene            complement(1044..>1160)
                     /locus_tag="SCK20_002898"
     CDS             complement(1044..>1160)
                     /locus_tag="SCK20_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1146895.1"
                     /translation="TIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          383 a          195 c          195 g          387 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataagg gttctgttgc aaagttttaa ataaagaata
     1021 aaatccctta cggtatctat gatttaagct gggattccca ataatacctt gatttcagta
     1081 cagaccgaaa acccgaagag agtgccttct tttcgggttt tcttatataa tcctcggatg
     1141 gcttccatgc ctttaatcgt
//
LOCUS       DARBEG010000224         1143 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000224 DARBEG010000000
VERSION     DARBEG010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.233"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="SCK20_002899"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="SCK20_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG1146896.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DARBEG010000225         1105 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000225 DARBEG010000000
VERSION     DARBEG010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.234"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SCK20_002900"
     CDS             <1..1087
                     /locus_tag="SCK20_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1146897.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DARBEG010000226         1090 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000226 DARBEG010000000
VERSION     DARBEG010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.235"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            266..>1090
                     /locus_tag="SCK20_002901"
     CDS             266..>1090
                     /locus_tag="SCK20_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1146898.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKL"
BASE COUNT          385 a          152 c          185 g          368 t
ORIGIN      
        1 gtataattta aaggtaataa gttattcttt tattactatg tttgggtttt atagcaaaga
       61 aagtttaata agagtttgga cctagcaaac aatattaatg aggtcattaa atcttattat
      121 ctttagtgat ataacgaaat atctacacag acaaaatgat agcgtaaaat attttgtgta
      181 aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac
      241 cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta tggaaacact
      301 aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc
      361 attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt
      421 caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga
      481 attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc
      541 ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat
      601 tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca
      661 aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc
      721 cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca
      781 aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga
      841 ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca
      901 agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg
      961 cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
     1021 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
     1081 tttcaaatta
//
LOCUS       DARBEG010000227         1073 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000227 DARBEG010000000
VERSION     DARBEG010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.236"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1073
                     /locus_tag="SCK20_002902"
     CDS             <1..>1073
                     /locus_tag="SCK20_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1146899.1"
                     /translation="ITTQAEYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDIN
                     EFMSSVLSLATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSY
                     FTINFNHFKYLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYL
                     RIMIKGAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYI
                     SNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSID
                     LAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNI
                     LLNMIFIPRLGIIAAGYTTVFTY"
BASE COUNT          328 a          137 c          180 g          428 t
ORIGIN      
        1 atattactac tcaagcagaa tatggtttga ttaataattt cacttcattt gtatcaattt
       61 tttcgattct tgttgggcta agcctaaatt cggcaattaa taatgcaagt tttgactata
      121 aagatgacat aaatgaattt atgtcatcgg tactttctct agctacactt tcgttaattg
      181 tgttcttatt aatagggaac atatattttt tgttttcaaa tgcttttttt gatatgaacc
      241 aacttgtatt taatttgatg ttattacaaa gttttgctag ttttgtaatt agttatatcc
      301 agtcttattt tacgattaac tttaaccatt ttaaatattt gattttatct tttatatcta
      361 cttttttgaa tattgcaatc tctgtagctt tgatgcagac attatatttt gatgataaat
      421 atgttggcag agtagtcggc ggaacactgt catttgtgtt gataggttta gtaatttatt
      481 taagaataat gataaagggt gctcattttt ttgataaaaa acagtggaga tacgctttaa
      541 aaatctcttt gccgctaatc ccacattcct tagcaaatat agttttagca caattcgata
      601 gaattatgat taattcttat ttaggtcctg ctgcgggggg aatatattcc tacattagta
      661 acataggtat tattctaagc gtaatatgga tgtctactaa taacgcctgg gttccatggt
      721 tctatggtga gatgagtaaa aataatgaaa aaaaagtgaa gaaagttagt aactattatt
      781 tagtagtctt tactttgatt acgatgattg taatggtagt ttcgattgat ttagcaaaat
      841 ttatggctcc gaaagactat tattctgggt tatctttagt ggttccgata accttaggct
      901 atttctttca gtttttatat agtttaccag ttaatgctga attttatgag aaaaaaacat
      961 cgtttattgc gattggtaca gtagcaagtg ctatagttaa tatactatta aacatgattt
     1021 tcatacctag gttaggaata attgctgctg gatacacaac cgtatttacg tat
//
LOCUS       DARBEG010000228         1069 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000228 DARBEG010000000
VERSION     DARBEG010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.237"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..969
                     /locus_tag="SCK20_002903"
     CDS             <1..969
                     /locus_tag="SCK20_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1146900.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DARBEG010000229         1051 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000229 DARBEG010000000
VERSION     DARBEG010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.238"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="SCK20_002904"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="SCK20_002904"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG1146901.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DARBEG010000230         1010 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000230 DARBEG010000000
VERSION     DARBEG010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.239"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..848
                     /locus_tag="SCK20_002905"
     CDS             <1..848
                     /locus_tag="SCK20_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1146902.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          329 a          195 c          202 g          284 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt
//
LOCUS       DARBEG010000231         1002 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000231 DARBEG010000000
VERSION     DARBEG010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.240"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            369..1002
                     /locus_tag="SCK20_002906"
                     /pseudo
     CDS             369..1002
                     /locus_tag="SCK20_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DARBEG010000232          995 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000232 DARBEG010000000
VERSION     DARBEG010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.241"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..913)
                     /locus_tag="SCK20_002907"
     CDS             complement(26..913)
                     /locus_tag="SCK20_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG1146903.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DARBEG010000233          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000233 DARBEG010000000
VERSION     DARBEG010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.242"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="SCK20_002908"
     rRNA            complement(<1..>994)
                     /locus_tag="SCK20_002908"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DARBEG010000234          953 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000234 DARBEG010000000
VERSION     DARBEG010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.244"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..639
                     /locus_tag="SCK20_002909"
     CDS             1..639
                     /locus_tag="SCK20_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1146904.1"
                     /translation="MFSGKQYADNILLWGGEDNIEKINNNLTILDTALENKEQKISIL
                     NSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQN
                     EINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNNI
                     QNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          421 a          117 c          136 g          279 t
ORIGIN      
        1 attttctccg gaaagcaata tgctgataat attttattat ggggagggga agataatatt
       61 gaaaagatca acaacaattt aactatatta gacacagctc tagaaaataa agagcaaaaa
      121 attagtattt taaattcaca attgtcttca aacaaccaaa ctcttgaaca actaagaact
      181 aatattgaag tttccaaaca aaaagtggca tctttagaaa acgacaagaa tcaattagta
      241 tcagagaaaa ctaatttaga aaatcaattg tcagcaaaag actctgaatt gcaaggaaaa
      301 caaaatgaaa ttaatgcgaa aatagacgaa attaaccaaa aagtagctga aataaatcga
      361 ttaaatagcg attggaataa tcgtttacaa aatgaaaaga acaaatttaa tgaagaacaa
      421 tctactatta atagtttaaa ccagcaaatt aatcaaatgt tgagtgaaaa aaataacatt
      481 caaaatcaac tgcaagctac tcagcaaaag ctagaagaag aaaagtcaaa aaatagtagt
      541 ttgaataatt acattgaaaa gcttcaaaaa gctaaaaatg atgtagagga taccgcaaat
      601 aaagcacaac aaattgtaaa tgaacataca atccaataga tatagtttta atttaacaaa
      661 tggtatgttt ggagataatg agatagaata taaatgtcat aatattatac aatctaaaca
      721 ccgtttcctt ttctattttt gatgacatca gtatatagct actcgaaaga gtagtctttt
      781 tattattctc gcttattatt gatatgaaaa tttcgatttg acaaaaggta ccgaataatt
      841 ttgtaatatg aattattatc aagaagtaat tatgatgata acatatggaa actagttagt
      901 aataataaca ggtgattaaa cttttgaacg acttgtaatt ctatattttt tct
//
LOCUS       DARBEG010000235          931 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000235 DARBEG010000000
VERSION     DARBEG010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.245"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>931
                     /locus_tag="SCK20_002910"
     CDS             <1..>931
                     /locus_tag="SCK20_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1146905.1"
                     /translation="HCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFY
                     RPLNPFKQTLPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFS
                     RFSLATREAVQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETT
                     VRLKGNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAA
                     CPNLRQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNA
                     LHYSYSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          286 a          190 c          180 g          275 t
ORIGIN      
        1 acactgtttc attagtcaaa aagttcgttg gtctgtcatt gctcgcttaa aagagaatac
       61 ttccatgacg gaaatcgctc gtcagaaaaa tatttccact tcttcggtct atcgtgtcat
      121 gaagcgattc taccgcccat tgaatccttt caaacagact ttgccaaagg tcttatgctt
      181 tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg agttttatca tgatggacgg
      241 acaaagccat gcattattgg atattgtcga aaatcgacgc ttgccttatt tagaacgcta
      301 tttttctcgg ttttcattag cgactcgtga agccgttcaa ttgattgtta ttgatatgta
      361 tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat gctcagttga tcatcgatcg
      421 tttccacatc gtccaacata ttggcagaac tttcttgaat catcgagtga aagaaaccac
      481 ggtacgactt aaaggaaatt cccactctca acgagggtta gggaaaaaat tgaagcgcta
      541 ttggaaactc ctacaaaaag aagaagcaaa gttaaactat gaaaaacggg tttggcgtgc
      601 cagttttaaa gattatttga ctgaaagtga gattgccgat cgactgttag ctgcttgtcc
      661 caacttacgc caaggctatc agttatacca agacattctt tatgcggtaa agaaaagaga
      721 tcagtcacta tttgaggatt gtttaactag agacattagc ggtctgccag aaacctatac
      781 aaccacttta cgaacgttta aaaagtttct gccacagatc caaaatgcgc ttcactacag
      841 ctattcaaac ggaccattgg aatgcttaaa caaccacatc aaagtattaa aacgcaatgc
      901 gtacggcttt cgcaactttt acaatttcaa a
//
LOCUS       DARBEG010000236          915 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000236 DARBEG010000000
VERSION     DARBEG010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.246"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..355
                     /locus_tag="SCK20_002911"
     CDS             86..355
                     /locus_tag="SCK20_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1146906.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="SCK20_002912"
     CDS             348..605
                     /locus_tag="SCK20_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1146907.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          352 a           99 c          174 g          290 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta ctaaaggatg acgcttatat atatgactga atagaataag
      901 caataggttt aataa
//
LOCUS       DARBEG010000237          907 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000237 DARBEG010000000
VERSION     DARBEG010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.247"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>907
                     /locus_tag="SCK20_002913"
     CDS             <1..>907
                     /locus_tag="SCK20_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG1146908.1"
                     /translation="EKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMV
                     DEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMN
                     AAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNW
                     RFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEA
                     FRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHI
                     KTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          305 a          176 c          170 g          256 t
ORIGIN      
        1 tgaaaaagta tctttatctt ttattgcgaa aaattgtcag gtatctttga ccaccgttat
       61 tcgtacgttg aaagagttta aaagctactt accaaagcaa tctaagagga ttctccccag
      121 agtattgatg gttgatgaat ttcgttcgca cgcttccata gaagataaaa tgagctttat
      181 ttgcgcagat ggcgaaacag gacaattaat tgatgtcttg cctacgcgta aattgcctcg
      241 attaacaagc tatttcttag gctgcgctaa tcctgaagaa gtaaaatttt tagtcacaga
      301 catgaacgcc gcctacttcc agcttaccaa acgtattctg ccaaatgcga aagtagtgat
      361 tgatcggttt catattatca aacacatgaa tcaagccttt aatgagttgc gtatccgtga
      421 aatgaatgaa cttcgtaaag taggacagaa aagtcaggca gaaaaactga aaaagaactg
      481 gcgctttttg ctaaaaaatc gtgcaaacat caaccattat gaatacaaaa catggaaaag
      541 ttttcgagca ccaaaatacc catttcttac ggaagcgatg atgattgatc gattgcttgg
      601 cttttctacg tccctgaagg aggcgtatcc ctattttcat gaattagttg aagcctttcg
      661 agacaaagac cctgacttat ttttctcctt attggcagaa cttcccgaaa cgttagatga
      721 cggctttcgg gaaaagctcc aaaaccttct gacctatgaa gaaggcatca ccaacgcgat
      781 gatctatcct tacaccaatg gaaaaataga agcgaagaat acccatataa agacaatgaa
      841 acgagtatcc tacggattta aatcatttga gaacatgaga attagaatct ttttgatcaa
      901 tcaatta
//
LOCUS       DARBEG010000238          905 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000238 DARBEG010000000
VERSION     DARBEG010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.248"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            265..888
                     /locus_tag="SCK20_002914"
     CDS             265..888
                     /locus_tag="SCK20_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1146909.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          334 a          129 c          167 g          275 t
ORIGIN      
        1 tagttttaaa ataaactaat gtccttattt ttgtgttcac atttctgtta cctaagaaat
       61 acttaaagga ggtgatgtag ccagcaacga ctacaacttg aacccgctat ctacagttta
      121 tttgttgttt cttttgggta tactctaatg gtacaaatcc atatttgctt aaagcgttga
      181 tttttcagcg gtttttcttt attgcaaagt gtaccctaag acctgtatac taatagtaaa
      241 tagattggat aggaatgatg gcaaatgtat ttagcttatt taagagtttc ttccgacgat
      301 caaagtttag ctcggcaaca taagctaatt gaaaattggt cagtcaaaaa taatattgct
      361 gaagacgatt taaaaatttt cgaggaaaaa gtctctggaa aaaatattga aaacagacca
      421 gagctgcaag agcttatttc tttcatacgt gaacgagata cgattattat tcctagtctt
      481 gatcgattag caagaaatag taaagataca aaagacttac ttcaattgtt tcgtagtaaa
      541 ggtgcttcta ttgaaatatt agacctaccg tcgtttaatg gggtaactga ccctgcatta
      601 agagacttgc taacaaattt agtcatagaa gtgtttagct atgttgctga aaacgaacgg
      661 aaaaaaatca aagagcgtca aaaacaaggc attgaaattg caaagaaaaa tggtcgttat
      721 actggtaaaa agattcaata ccatgccaat gcaacaggaa aaaacaaatt gatttacgat
      781 gaaattgttg atggtctgaa gaaaaaagaa actatttcta aaatagcaaa gaacgcagga
      841 gttacaagaa aaaccgttta tcgagtaaaa agagatttag gaatgtaata tgttccagtt
      901 agaaa
//
LOCUS       DARBEG010000239          890 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000239 DARBEG010000000
VERSION     DARBEG010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.249"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..888
                     /locus_tag="SCK20_002915"
     CDS             <1..888
                     /locus_tag="SCK20_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG1146910.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          116 c          144 g          244 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt actgggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DARBEG010000240          873 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000240 DARBEG010000000
VERSION     DARBEG010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.251"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..132)
                     /locus_tag="SCK20_002916"
     tRNA            complement(60..132)
                     /locus_tag="SCK20_002916"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:His,seq:gtg)
     gene            complement(135..208)
                     /locus_tag="SCK20_002917"
     tRNA            complement(135..208)
                     /locus_tag="SCK20_002917"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(174..176),aa:Trp,seq:cca)
     gene            complement(215..295)
                     /locus_tag="SCK20_002918"
     tRNA            complement(215..295)
                     /locus_tag="SCK20_002918"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(259..261),aa:Tyr,seq:gta)
     gene            complement(297..372)
                     /locus_tag="SCK20_002919"
     tRNA            complement(297..372)
                     /locus_tag="SCK20_002919"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(337..339),aa:Phe,seq:gaa)
     gene            complement(408..480)
                     /locus_tag="SCK20_002920"
     tRNA            complement(408..480)
                     /locus_tag="SCK20_002920"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Asp,seq:gtc)
     gene            complement(490..562)
                     /locus_tag="SCK20_002921"
     tRNA            complement(490..562)
                     /locus_tag="SCK20_002921"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(527..529),aa:Val,seq:tac)
     gene            complement(578..649)
                     /locus_tag="SCK20_002922"
     tRNA            complement(578..649)
                     /locus_tag="SCK20_002922"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(614..616),aa:Glu,seq:ttc)
     gene            complement(664..753)
                     /locus_tag="SCK20_002923"
     tRNA            complement(664..753)
                     /locus_tag="SCK20_002923"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(717..719),aa:Ser,seq:gga)
     gene            complement(793..866)
                     /locus_tag="SCK20_002924"
     tRNA            complement(793..866)
                     /locus_tag="SCK20_002924"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(830..832),aa:Asn,seq:gtt)
BASE COUNT          243 a          240 c          200 g          190 t
ORIGIN      
        1 cgcatgaggg agtcaaagtc ccttgcctta ccgcttggct ataccccaag aataaaaagg
       61 gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca atccggtgcg ttaaccactt
      121 cgccacattc gccatggcag gggcagcagg aattgaaccc acactaacgg ttttggagac
      181 cgctgttcta cctttaaact atgcccctat aaaatggagg agagtggatt cgaaccactg
      241 aaccctaagg aacggattta cagtccgtcg cgtttagcca cttcgctact cctccatggt
      301 ggcgcgggac agaatcgaac tgccgacaca cggagcttca atccgttgct ctaccaactg
      361 agctaccgcg ccatatttta aaattattaa ttgaataaca agaacgacgg ttccgacggg
      421 aatcgaaccc gcgatctcct gcgtgacagg caggcatgtt aacccctaca ccacggaacc
      481 agttcattat ggaggttaac gggatcgaac cgctgaccct ctgcttgtaa ggcagatgct
      541 ctcccagctg agctaaacct ccaacattca attaaaatga cccgtacggg attcgaaccc
      601 gtgttaccgc cgtgaaaggg cggtgtctta accgcttgac caacgggcca ggtaaatttg
      661 ctacggagag taagggattc gaacccttga gacagtttgg caccatctac atgatttcca
      721 atcatgctcc ttcggcctct cggacaactc tccagagtga aagaagctca atactctttc
      781 tcaaaatgag aactccggca gtaggactcg aacctacgac atcatgatta acagtcatgc
      841 gctactacca actgagctat gccggaataa cat
//
LOCUS       DARBEG010000241          863 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000241 DARBEG010000000
VERSION     DARBEG010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.252"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..610
                     /locus_tag="SCK20_002925"
     CDS             <1..610
                     /locus_tag="SCK20_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1146911.1"
                     /translation="ENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMD
                     IIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFI
                     GLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTI
                     SEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            627..>863
                     /locus_tag="SCK20_002926"
     CDS             627..>863
                     /locus_tag="SCK20_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146912.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTL"
BASE COUNT          315 a           97 c          158 g          293 t
ORIGIN      
        1 agaaaacatg gtttttaatc tgagtaatgg aataattcca agtgttagtg gagatacgat
       61 tcggtctgaa aaaaattatt ctatcattgt ttttgaaaag ttagcacaaa cgtctataac
      121 attaggaatg gatataatag aagcgtatca aagtagagat gctttgatac aagaaaacga
      181 attagctgta tcgttacctg aggtcttaaa agtgagggat tctgggattg tttattatac
      241 taaggaaatc ggaaaaacta agatagagca tctttctccc ttaatttctt ctgtcgttca
      301 attcatcggc ttaaatatct ataaaagaat tactgtaaag gaaatagcta attatttttc
      361 tgtaagtgaa actaaattaa gagaaagctt taagaatgaa atgcatatta cgatttataa
      421 ttatatctct aagcgaaaaa tttctacggc taaaataatg ttgaaatcaa accatactat
      481 cagtgaagtt tctttaggtt taggattttc agattcttca cacttttcga gagtattcaa
      541 aaagtacgca ggtgtatctc ctaagcaata tcaattaggt ttagtggaca atttcaacaa
      601 cattagctga gaggttttat tttattatga gactatatca tttacgtagt ttggaggagt
      661 tgaggaaatt ggattttaac aaaaaatttt tttgggaaat ttctgatttg agttgtttcg
      721 tagaatctga gcgtctaagt aatggtaata atagtgtgtt aaatgaatat gacaaagtag
      781 taattaataa agtaaaaagt ttatgtgaag aagctagaga atctatcagt catattaatg
      841 gtagcaattt taaacaaaca tta
//
LOCUS       DARBEG010000242          854 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000242 DARBEG010000000
VERSION     DARBEG010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.253"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..>854
                     /locus_tag="SCK20_002927"
     CDS             33..>854
                     /locus_tag="SCK20_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146913.1"
                     /translation="MEIFGFYDTAFHINRALSLESIFSSPINFETFRSYGMQVNNFYP
                     WLTLYPLFLLIKFTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIYTT
                     SSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHVLS
                     VAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLN
                     RPYVDILQKRAIYLGKEFLIGSIDSELLSFGIGLTLLICLIILTFNFKKLGILSKI"
BASE COUNT          271 a          118 c          121 g          344 t
ORIGIN      
        1 gtttattata tttatttcca gcatttaaaa acatggaaat atttggcttt tatgatacgg
       61 catttcacat taatcgtgcg ttaagtttag aatctatttt tagctcccct attaattttg
      121 aaacgtttag atcatatggc atgcaagtaa ataattttta cccatggtta actctatatc
      181 ctttattttt attgatcaag tttactaatt tagcaatagg ctataacctt tttctttata
      241 ttgttacgct cattactcta tttatatgtc attatgtaat gtatgaaatt accaaaaaac
      301 atgttactag ctcctttttc gctattatat atacaacatc ttcttttagg agtgtagaaa
      361 tatttcttcg aggggcaatg ggggaattac tcgcaatgtc aatacttcct cttattttac
      421 tcgggtttat aaagctttac gactcaaaaa aagaatcttg ggttatgttg gcgattagca
      481 tgactttatt aatttacact catgttcttt ctgtagctat gactatggta atgattataa
      541 ttgctttaat tattcaattt tatcggaaaa agaaaataga aatcttctta attattaaat
      601 taataaaagc tgctatagtt actttacttc tttcactagc gtttataggt cctatgattg
      661 agcagacatt gtatcaagat ctgaatagac cttatgtgga tatattacaa aaaagagcaa
      721 tttatctggg taaagagttt ttgattggaa gtatagattc agaattacta tcatttggta
      781 ttggcttaac tttgcttatt tgtttaatta tactaacatt taactttaaa aaattaggaa
      841 tcttatctaa aatc
//
LOCUS       DARBEG010000243          823 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000243 DARBEG010000000
VERSION     DARBEG010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.254"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            361..759
                     /gene="spxA"
                     /locus_tag="SCK20_002928"
     CDS             361..759
                     /gene="spxA"
                     /locus_tag="SCK20_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1146914.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          305 a          113 c          148 g          257 t
ORIGIN      
        1 gaacagtatt atttaaaatg acagaaaaca ttactattaa agaaataaaa aacataaaaa
       61 atcttaagaa atcggtttca tttagctttt gagataaaat aatagtacag ttttatacgc
      121 tgtttcatat gtttagaaaa tttgaattag atttgaaaac tctaattttt aaatgataaa
      181 aaaagcgaga atgctggttt tactgtatat taaccttaga taactttcat ttttttaaaa
      241 aaattatgaa aaaaaggtag ctatttaaaa gttgattatg tataatagaa acagatggga
      301 gattattatg atcttctgtt acaaaaaagt gattcttatc acgaaaagga gtggtgcacg
      361 ttgttaacat tatatacttc cccaagttgt acttcctgca gaaaggctcg tgcgtggttg
      421 caagaacatc agattccatt tgttgaacgt aatatttttt cagaaccatt gaacagttct
      481 gaattaaaag cgattttaca aatgacagaa gacggaacag aagaaatcat ctcgacacgt
      541 tccaaagtat tccaaaaact gaacatggat ttagatgatc tgccgttaca agaattattg
      601 gaacttgtgc aaaacaatcc tggattgctt cgccgtccaa tcatgattga tgataaacgt
      661 ctgcaagttg gttttaatga agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa
      721 ttagaattac gtcaagcaca attgatggct ggattataga aggataatac gaaacttgga
      781 aaagactggc tctttgtcaa atgatgttgg taaagagaaa tct
//
LOCUS       DARBEG010000244          802 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000244 DARBEG010000000
VERSION     DARBEG010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.256"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>802
                     /locus_tag="SCK20_002929"
     CDS             <1..>802
                     /locus_tag="SCK20_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1146915.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHG"
BASE COUNT          337 a           73 c          132 g          260 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gt
//
LOCUS       DARBEG010000245          801 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000245 DARBEG010000000
VERSION     DARBEG010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.257"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            7..624
                     /locus_tag="SCK20_002930"
     CDS             7..624
                     /locus_tag="SCK20_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146916.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          310 a           99 c          147 g          245 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac g
//
LOCUS       DARBEG010000246          788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000246 DARBEG010000000
VERSION     DARBEG010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.258"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..>788
                     /locus_tag="SCK20_002931"
     CDS             226..>788
                     /locus_tag="SCK20_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146917.1"
                     /translation="MKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESK
                     SFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEFICFSMGEVWLEGIYACL
                     YRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKSYNFYCFFPNI
                     YTGRENTIQIPLVTAGSRTALQLSDIF"
BASE COUNT          306 a           88 c          128 g          266 t
ORIGIN      
        1 cacttatcca gacactcaaa tgatttttct cttagtacct atgatagttt cgtgggttga
       61 taatcaacaa cgaatatcgg aatagatagc taaagtatat tttaattatg aatttataat
      121 tagttgtaaa agttaatatt taacggacag gaagtagggt gaaaaaattt gaaaaaaatt
      181 ttagtgttag caaatacata ttatcaaata atagtagcaa tacaaatgaa aaatacaatt
      241 ttttccgatg agtatgttga aattattatt tctgatcatt ctaaaaacac taaagaaatt
      301 gcgaaaaaaa tagaagaaaa aaaagtgttt gatggctgtc attatttaga aagcaaatct
      361 tttgatgata atattgggta ttttcaaaag attaaggact gtcttcagat ttcttgtggt
      421 aaatcaaatc gatatgccta ttatttgaaa aactgtaaaa gtgaatattt tgatgaattt
      481 atttgttttt caatgggaga ggtttggttg gaaggaatat atgcttgctt atatcgaaat
      541 aataaaaatg tacaggtatc aattttagaa gaaggtattt taagctatgg agttgacatt
      601 attccaacag caagaacaaa agctatcgca gtactcaatt acttaaaaaa aaggactcca
      661 ctatataaca aaagctataa tttttattgt ttttttccta atatatatac aggaagagag
      721 aatacaatac aaattccatt agttacagct gggtcaagaa ccgcattgca actttcggat
      781 atttttaa
//
LOCUS       DARBEG010000247          746 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000247 DARBEG010000000
VERSION     DARBEG010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.259"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..>746)
                     /locus_tag="SCK20_002932"
     CDS             complement(109..>746)
                     /locus_tag="SCK20_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG1146918.1"
                     /translation="RGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTD
                     LTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTD
                     RGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQL
                     RLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          209 a          171 c          142 g          224 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggc
//
LOCUS       DARBEG010000248          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000248 DARBEG010000000
VERSION     DARBEG010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.260"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SCK20_002933"
     rRNA            <1..>733
                     /locus_tag="SCK20_002933"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARBEG010000249          725 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000249 DARBEG010000000
VERSION     DARBEG010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.261"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..693
                     /locus_tag="SCK20_002934"
     CDS             <1..693
                     /locus_tag="SCK20_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146919.1"
                     /translation="YLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYD
                     VKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKV
                     NSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISF
                     SPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENW
                     AELRKLVEYGII"
BASE COUNT          284 a           93 c          105 g          243 t
ORIGIN      
        1 tatttgaaga agagtatatt ttcaacaagt aaaaacgttc aaagagtttt taaaatatta
       61 aataaaaata gcatcttcaa aaaaaaaatt gacgattatt ttgtctattg tccggaattg
      121 ttactttatg atgtaaaaaa tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg
      181 atgaaaaaca tacaatcaat ctttgggata gaagaagttt ttgtcggaga taaatgtatc
      241 ttttttgatc aacctttatt atctgatggt tatggcatag atgaaacaga tattttcgct
      301 aaagtaaata gtatattttc tggaaaaaaa gaaactttaa ttaagcttca tcctagagca
      361 aggacaaatc gttatggtga aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg
      421 ctacttgaaa atgacttctc ttcaacactt ttgattagtc ctatatctac aatttctttt
      481 tcaccatcta tgatgtttga aaagacgatc aactctatta tgttggctaa attaatttta
      541 aatgaatata cagcattatc tgaagataaa aaagaaacat tgacaaatat tgttcttttc
      601 tgtgaacatt ataaccgtct atcagaagaa aaaattttac tcccagaaaa ctgggcagaa
      661 ttaagaaagt tggtagagta tgggattatt taacacactt tccaagtcaa aattagttaa
      721 gtcca
//
LOCUS       DARBEG010000250          724 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000250 DARBEG010000000
VERSION     DARBEG010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.262"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            403..666
                     /locus_tag="SCK20_002935"
     CDS             403..666
                     /locus_tag="SCK20_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146920.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT          293 a           84 c          124 g          223 t
ORIGIN      
        1 tataatcact ttaataaatt gttggtagaa ggtgatcaaa atgggagtaa acgaaatgat
       61 gaaagtcaga ttgagttttt aaatgatacc ttcctcagaa cttcaaaacg aattcaaaga
      121 ttcgttgaca aatcttgagc aagatcattt aaaaaaatat tttctcttca tttgacgaaa
      181 aatgtttaga aatcttatat aagtgaagat aaagtaattg gtgtaattaa ttgtagagaa
      241 tagcctttga gcaattgaag cgatacaatt atttagaaaa tgaacttcct ccagcatact
      301 acatgattcc ttacgggacg atgttaaaga agaaagcaag aaattttaga agtattagta
      361 gataaactaa tgggcttttc tcaatttaag ggagtagccc aaatgtttat caaatatggt
      421 gcagatacag tagcaaccat gtttacagaa gaatatcaag tagactatta tgaagtaata
      481 gataaatgtg tacattttgt ttatgaaaaa gtgtggaatt ttatgaattt tgcgataagt
      541 gaagaaatct tacgagtaca agaaaacaga acaggaatat ttaccagaga cagaaagctt
      601 aatttgattg cgcattatat acggaatcgc tggtcatcaa taccaaattt taattataaa
      661 aaataattat gaacaatctt ttatacaata tacacaataa atcaagtagt atttatgaaa
      721 aaag
//
LOCUS       DARBEG010000251          712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000251 DARBEG010000000
VERSION     DARBEG010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.263"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SCK20_002936"
     CDS             complement(<1..446)
                     /locus_tag="SCK20_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146921.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARBEG010000252          710 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000252 DARBEG010000000
VERSION     DARBEG010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.264"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..448)
                     /locus_tag="SCK20_002937"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="SCK20_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT          215 a          141 c          105 g          249 t
ORIGIN      
        1 cagacacttg cttaaagcga aaaatttctt caatgtccat gcctaattct ttggatattc
       61 tcttgtctga ccatccattt tttactaatc tttggatgac aatttccatt cctcttattt
      121 catgggtccc tcttgcacga ttgtgtcgaa ttgttgaagc catacgttct tatatgggtt
      181 tatcaataac tgttaccggt acaactttta agttgagtct ttccttagca acacgatacc
      241 tatgaagcac atcaaccaag atatacttct cttctttttc cttgtaataa caaactaatg
      301 gttgagtaaa gccatcttca atgatagaaa gctccaataa gttcatttcc ggagacgcta
      361 ctcgattggg attataatcg ttcgcctgta ttttggtaat tggaacaagc cgaacgtcca
      421 tcacagggaa agttatgtct ttattcatac gcttgtatac cctactttct taaaaagtag
      481 ggtatactca agaatccgat taattatctt ctcaatttgg ataatatatc tcatcagtct
      541 tcttgtcact ttatcaacat gattttgctg caaaatggga taccgacagt actggactgg
      601 taatcaggat aaaagcaaaa ataattaatt tgtatcctaa aaaatccata aaaattctct
      661 cacattttcg ggtattaatt cgagaatcac acctataaat ttattactat
//
LOCUS       DARBEG010000253          704 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000253 DARBEG010000000
VERSION     DARBEG010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.265"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          298 a          106 c          122 g          178 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactctc gatgaagtag acctttgaaa actgaacaaa
      541 gtaagacaaa ccaaatgtgt agggcgtctt gattcaattc aagacaacaa acatttttaa
      601 caagcaagca atatgctagc aaacaaattg agcttaacaa tcgcaagatt gttcgaactt
      661 tttatgagag tttgatcctg gctcaggacg aacgctggcg gcgt
//
LOCUS       DARBEG010000254          702 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000254 DARBEG010000000
VERSION     DARBEG010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.266"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..344
                     /locus_tag="SCK20_002938"
     CDS             132..344
                     /locus_tag="SCK20_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146922.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>702
                     /locus_tag="SCK20_002939"
     CDS             506..>702
                     /locus_tag="SCK20_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG1146923.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDIL"
BASE COUNT          252 a          104 c          122 g          224 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aa
//
LOCUS       DARBEG010000255          665 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000255 DARBEG010000000
VERSION     DARBEG010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.268"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..124
                     /locus_tag="SCK20_002940"
     CDS             <1..124
                     /locus_tag="SCK20_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1146924.1"
                     /translation="TFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            447..563
                     /locus_tag="SCK20_002941"
     CDS             447..563
                     /locus_tag="SCK20_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEG1146925.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          276 a           73 c           86 g          230 t
ORIGIN      
        1 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
       61 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
      121 ttgacaaatc ataaataata aagaattgaa cttctgagaa aacaaagaat cttttttctt
      181 tttttgaatt gactcatgga ttggcgttaa aattttattt tgaaaaagtt atttctggtt
      241 taactcaaaa aaagagatat gaatctaata gtgatagaaa ggatgttatt tttcatgaaa
      301 tggttgtttt agatttaata catataacta atgaatgcta ttgaatgtat ttaaactaat
      361 taacaaaaga atttgaattg ttaataaaat ccaataaaat atattaataa agtagataga
      421 aataaaaaaa caaagactaa acatatataa ttggattaaa cgcaatatct gcaaaatatt
      481 gtaaatttaa agcctttgta gaaaaattta ctgaaagaca aactgataag attattcagt
      541 atcatattac ctatactgat taaaattttt tgaaacggga tactaaataa aaaatttatt
      601 tataacagaa ctaattattt aatgcggatg cgccaatact tggtcctgca agagctattt
      661 atttt
//
LOCUS       DARBEG010000256          662 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000256 DARBEG010000000
VERSION     DARBEG010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.269"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            122..457
                     /locus_tag="SCK20_002942"
                     /pseudo
     CDS             122..457
                     /locus_tag="SCK20_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            553..>662
                     /locus_tag="SCK20_002943"
     CDS             553..>662
                     /locus_tag="SCK20_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146926.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          234 a          127 c          132 g          169 t
ORIGIN      
        1 tttatataat aaaatgcaat acttttcgag agtgtaaaat attttgtgta aatagaaaaa
       61 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
      121 tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga
      181 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
      241 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acgggtaatt ctcgtaacgg
      301 ctcctatgac cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga
      361 ccgcaacggc gagttcaagc aacagactgt tcctgcttat agacggacga atgatacctt
      421 agaggagacc gtcattcacc tcttccgaaa aggtatggat aggctacaat taactagtgg
      481 ataggctaca attaactaga cagaaaaatt aaggtgtgta gactagaaga aaacatacca
      541 ggaggaattt ttatgtctaa gagaacacga agaacttttt cacaagaatt caagcaacaa
      601 atcgtcaatc tttacttagc tggaaagcca cgtgtagaaa tcattcgaga atatgaacta
      661 ac
//
LOCUS       DARBEG010000257          657 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000257 DARBEG010000000
VERSION     DARBEG010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.270"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          229 a          111 c          108 g          209 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgac
//
LOCUS       DARBEG010000258          639 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000258 DARBEG010000000
VERSION     DARBEG010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.271"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            60..548
                     /locus_tag="SCK20_002944"
     CDS             60..548
                     /locus_tag="SCK20_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1146927.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          257 a           53 c          114 g          215 t
ORIGIN      
        1 gtttttatgt gaaaacatga gcaatagaat tgtactagta ggataaggat ggatggaaaa
       61 tgaaaaaaac tatttttgtt ttagcgattt ttatatgttt tgggctactt ttggtaggct
      121 acttgttgtt taattctaat gaaatgagaa ctatttctac gaatgacttg ttaaaaaaaa
      181 gtgaattaaa ggataaaaaa aaagaatctc ttgcctttat aatgtttact caagaagagt
      241 gtgagtattg tatagcaata aatccaatca tagatagtgt aaataaggaa actttgaata
      301 agataaagaa atatgatata tctgtagatt ttcaatctaa acaaaagtgt aaaattaagt
      361 ttgttccgac tattgttgca tatagtaaga ataaagaagt ggggagaatt gacaatgaat
      421 tagataattt gtatcgaaaa aagcaagatg gttcaattgt aatagacaag aaaaaaacaa
      481 agacattgct tatagattgg atgaaaaaaa tggataaaca aggagaatat ttgtatggag
      541 aagattaaac agacatttcc gttaattaat aaatattgga ataagaaaaa aatttttata
      601 ttttttatta cgtttctgct gtcatcttta ttttctcta
//
LOCUS       DARBEG010000259          635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000259 DARBEG010000000
VERSION     DARBEG010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.272"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..270)
                     /locus_tag="SCK20_002945"
     CDS             complement(<1..270)
                     /locus_tag="SCK20_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146928.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="SCK20_002946"
     CDS             complement(276..>635)
                     /locus_tag="SCK20_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1146929.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DARBEG010000260          608 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000260 DARBEG010000000
VERSION     DARBEG010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.273"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            178..381
                     /locus_tag="SCK20_002947"
     CDS             178..381
                     /locus_tag="SCK20_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG1146930.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..>608
                     /locus_tag="SCK20_002948"
     CDS             381..>608
                     /locus_tag="SCK20_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1146931.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDND"
BASE COUNT          237 a           65 c          102 g          204 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgat
//
LOCUS       DARBEG010000261          588 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000261 DARBEG010000000
VERSION     DARBEG010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.274"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..150)
                     /locus_tag="SCK20_002949"
     tRNA            complement(65..150)
                     /locus_tag="SCK20_002949"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="SCK20_002950"
     tRNA            complement(167..238)
                     /locus_tag="SCK20_002950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="SCK20_002951"
     tRNA            complement(250..322)
                     /locus_tag="SCK20_002951"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="SCK20_002952"
     tRNA            complement(340..421)
                     /locus_tag="SCK20_002952"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="SCK20_002953"
     tRNA            complement(429..501)
                     /locus_tag="SCK20_002953"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="SCK20_002954"
     tRNA            complement(505..577)
                     /locus_tag="SCK20_002954"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DARBEG010000262          569 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000262 DARBEG010000000
VERSION     DARBEG010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.276"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            28..>569
                     /locus_tag="SCK20_002955"
     CDS             28..>569
                     /locus_tag="SCK20_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEG1146932.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYR"
BASE COUNT          205 a           92 c          114 g          158 t
ORIGIN      
        1 tagaaaaaga tagcaggagg aatgacgatg aatcatttta aaggaaagca atttcagcag
       61 gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta tcgtgaagtt
      121 caagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta tcgttgggtg
      181 caagaatatg gcaaactact ctatcaaatt tggaaaaaga aaaataaaaa atccttttat
      241 tcatggaaaa tggatgaaac gtacatcaaa attaaaggaa aatggcatta tttgtatcga
      301 gccatcgatg cagatggttt aaccttggat atttggttac gtaaaaaacg ggacacacaa
      361 gcagcctatg cttttcttaa gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc
      421 acagataaag ccccctctat tacaagtgcc tttaagaaac taaaagaata cggcttttat
      481 caagggacag aacatcgtac cattaaatac ctgaataatt tgattgaaca agaccatcgt
      541 ccagtaaaga gacgcaataa attctatcg
//
LOCUS       DARBEG010000263          552 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000263 DARBEG010000000
VERSION     DARBEG010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.277"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..486)
                     /locus_tag="SCK20_002956"
     CDS             complement(<1..486)
                     /locus_tag="SCK20_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146933.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RE"
BASE COUNT          187 a           86 c           77 g          202 t
ORIGIN      
        1 ttctctaaaa aaagaaacat caagtggaaa ttcatcagct ttattaatag tgtaatgcaa
       61 aagtttaaaa tgtattttgg ttaactctct tcggatcaat tggctcgtct cttctcgcaa
      121 cagattaaac tgattttcaa aactttcaat gaatcgttca ttcaaccatt cgattgtttc
      181 atcttcattt tttctcaagt atttttgatc caatttatca tgaagataaa gccaagagag
      241 tgtcttattg ataaaaataa cggatttttc tcctaatact ccttttaagt ggtaaaatcc
      301 tatatccgaa aaaaatagcg actgattgct agcaatatct aaagaatcag cttgttcagc
      361 caacaggttg attgctaaaa agtggaataa tttctttttt tcataagcag acagaggata
      421 atactgtttt aaatctttta acattttttc tgtttttata attataggta gcgtatacaa
      481 ttccacaaaa cttatctctg ttgagttaaa aagattatat aaaaaaagcc gaatgttttt
      541 aggattccct gt
//
LOCUS       DARBEG010000264          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000264 DARBEG010000000
VERSION     DARBEG010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.278"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCK20_002957"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCK20_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG1146934.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SCK20_002958"
     CDS             complement(349..>526)
                     /locus_tag="SCK20_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146935.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARBEG010000265          516 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000265 DARBEG010000000
VERSION     DARBEG010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.279"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..>516)
                     /locus_tag="SCK20_002959"
     CDS             complement(107..>516)
                     /locus_tag="SCK20_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1146936.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          172 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccat
//
LOCUS       DARBEG010000266          514 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000266 DARBEG010000000
VERSION     DARBEG010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.280"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..132)
                     /locus_tag="SCK20_002960"
     CDS             complement(<1..132)
                     /locus_tag="SCK20_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1146937.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DARBEG010000267          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000267 DARBEG010000000
VERSION     DARBEG010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.281"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SCK20_002961"
     CDS             complement(62..259)
                     /locus_tag="SCK20_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1146938.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARBEG010000268          479 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000268 DARBEG010000000
VERSION     DARBEG010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.282"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            22..>479
                     /locus_tag="SCK20_002962"
     CDS             22..>479
                     /locus_tag="SCK20_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEG1146939.1"
                     /translation="MIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGKTALDYWH
                     QYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVSCGMRTLYRLFSK
                     GIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEG"
BASE COUNT          173 a           85 c           94 g          127 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggcctgtttc atgcggtatg
      301 agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa
      361 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
      421 atccatgatc gacctgataa ttatcctgat ttcaattctg agtttggtca ccttgaagg
//
LOCUS       DARBEG010000269          479 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000269 DARBEG010000000
VERSION     DARBEG010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.283"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            143..400
                     /locus_tag="SCK20_002963"
     CDS             143..400
                     /locus_tag="SCK20_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146940.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          204 a           63 c           68 g          144 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaata
//
LOCUS       DARBEG010000270          466 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000270 DARBEG010000000
VERSION     DARBEG010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.284"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          170 a           66 c           55 g          175 t
ORIGIN      
        1 tcctaatttt gctccacttt tagataattt ttaggtctat ttaactcatc agacattcta
       61 tgctcatttt tctccaaatt tgtttcaact ctttcgtact taattttgcg tatttgaaaa
      121 ttcttctttt agagtacttt tattacgata ctagtatgat atatatctat agcaaacagt
      181 taaaatcttc ctaaaataag cgaatagcat tcctaaaacc tataaaattt ttagatgaag
      241 cgacaagtgg acaattaata aactacttga acatgaaatt atggatcacc tttcaaattt
      301 agaaaatgga attcttatac ttattgcgca acatttattg attgcaaaat tatgtgacaa
      361 aaaaaactag tagaagaaag aataatggct tgataataaa gtactaccta ttaaaggtag
      421 tactttattt aatttagtat aaattaacat actactatat tttagg
//
LOCUS       DARBEG010000271          462 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000271 DARBEG010000000
VERSION     DARBEG010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.285"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..430)
                     /locus_tag="SCK20_002964"
     CDS             complement(<1..430)
                     /locus_tag="SCK20_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1146941.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DARBEG010000272          454 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000272 DARBEG010000000
VERSION     DARBEG010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.286"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..>454)
                     /locus_tag="SCK20_002965"
     CDS             complement(37..>454)
                     /locus_tag="SCK20_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146942.1"
                     /translation="KRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAA
                     YNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDW
                     GNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          137 a           83 c           75 g          159 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg tttt
//
LOCUS       DARBEG010000273          399 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000273 DARBEG010000000
VERSION     DARBEG010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.288"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          111 a           98 c           76 g          114 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttgg
//
LOCUS       DARBEG010000274          398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000274 DARBEG010000000
VERSION     DARBEG010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.289"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            122..>398
                     /locus_tag="SCK20_002966"
     CDS             122..>398
                     /locus_tag="SCK20_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1146943.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCK"
BASE COUNT          129 a           71 c           72 g          126 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagt
//
LOCUS       DARBEG010000275          395 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000275 DARBEG010000000
VERSION     DARBEG010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.292"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..390
                     /locus_tag="SCK20_002967"
     CDS             <1..390
                     /locus_tag="SCK20_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146944.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          155 a           72 c           60 g          108 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaat
//
LOCUS       DARBEG010000276          360 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000276 DARBEG010000000
VERSION     DARBEG010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.295"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..353
                     /locus_tag="SCK20_002968"
     CDS             <1..353
                     /locus_tag="SCK20_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEG1146945.1"
                     /translation="RFYNRVIGIGNIIKIVLSASIPILIDQASEHRSLLLIVSIASAI
                     ITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITKTSPYNKTDEENFHLIVTTLQT
                     ELNDIISDFNQVNN"
BASE COUNT          144 a           49 c           45 g          122 t
ORIGIN      
        1 ttcgatttta taatagagta attggtatcg gaaatattat aaaaatagtc ttatcagcta
       61 gtatcccaat attaattgat caggcttccg aacacaggtc tttactattg attgtttcaa
      121 ttgcttcagc aataattaca attatacaaa gtggtatgtc tgcatttaat tatcaagata
      181 aagtacagac ttcgacagag ttattaatga aaattgaaaa agaaaagttg ttatatataa
      241 cgaaaacatc cccatataat aaaacagatg aagaaaattt ccatttaatc gttacaaccc
      301 ttcaaactga attaaatgat attatctctg attttaatca agttaataac taaaaattta
//
LOCUS       DARBEG010000277          345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000277 DARBEG010000000
VERSION     DARBEG010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.296"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..321)
                     /locus_tag="SCK20_002969"
     CDS             complement(<1..321)
                     /locus_tag="SCK20_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1146946.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           63 g          130 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaat
//
LOCUS       DARBEG010000278          341 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000278 DARBEG010000000
VERSION     DARBEG010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.297"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>341
                     /locus_tag="SCK20_002970"
     CDS             <1..>341
                     /locus_tag="SCK20_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146947.1"
                     /translation="EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEP
                     DAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNR
                     SDADKYEPTVEG"
BASE COUNT          141 a           50 c           76 g           74 t
ORIGIN      
        1 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
       61 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      121 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      181 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      241 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      301 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DARBEG010000279          338 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000279 DARBEG010000000
VERSION     DARBEG010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.298"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..338
                     /locus_tag="SCK20_002971"
                     /pseudo
     CDS             1..338
                     /locus_tag="SCK20_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a           59 c           64 g           73 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcc
//
LOCUS       DARBEG010000280          331 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000280 DARBEG010000000
VERSION     DARBEG010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.299"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>331
                     /locus_tag="SCK20_002972"
     CDS             <1..>331
                     /locus_tag="SCK20_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HEG1146948.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DARBEG010000281          321 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000281 DARBEG010000000
VERSION     DARBEG010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.301"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(69..321)
                     /locus_tag="SCK20_002973"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="SCK20_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DARBEG010000282          308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000282 DARBEG010000000
VERSION     DARBEG010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.302"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(76..>308)
                     /locus_tag="SCK20_002974"
     CDS             complement(76..>308)
                     /locus_tag="SCK20_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146949.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           94 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaata
//
LOCUS       DARBEG010000283          307 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000283 DARBEG010000000
VERSION     DARBEG010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.303"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCK20_002975"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCK20_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG1146950.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           96 a           45 c           50 g          116 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttat
//
LOCUS       DARBEG010000284          288 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000284 DARBEG010000000
VERSION     DARBEG010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.304"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..>288
                     /locus_tag="SCK20_002976"
     CDS             18..>288
                     /locus_tag="SCK20_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1146951.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTP"
BASE COUNT          108 a           53 c           57 g           70 t
ORIGIN      
        1 aaggagagtg aacataaatg acttataccc atcttacacc aaacgagctt gtaatgatag
       61 aagcatattt tcatcaagaa actccggttg ctatcgttgc gaagcagctt aaacgtggac
      121 gccaaacaat ttacaatgtc tataactttc tcaaatgtgg tggaacagca cttgaatact
      181 ttgaacaata caaagaaaat aagcgacgtt gtgggagaac cgaaatcatt tttcctgctg
      241 aggaaaaaga atacattgaa aaaagatcaa ctgaaggttg gaccccag
//
LOCUS       DARBEG010000285          285 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000285 DARBEG010000000
VERSION     DARBEG010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.305"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..285
                     /locus_tag="SCK20_002977"
                     /pseudo
     CDS             1..285
                     /locus_tag="SCK20_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          110 a           58 c           56 g           61 t
ORIGIN      
        1 acaacaaatc gtcgatctct atctcgctgg taagcctcgc gcagaaatta ttcgagagta
       61 tgagcttacg ccttcttctt tcgataaatg gatgaagcaa gcacaatcaa cgggctcatt
      121 caaagaaaga gacaacttaa caccagaaca agcagaattg atcgcactaa gaaagaaaaa
      181 taagcaactc gaaatggaga atgatatttt aaagcaagcg gcgctgatat tcggacgaaa
      241 agacaagtaa ttgatgccaa caagcataaa tattccatat cagcg
//
LOCUS       DARBEG010000286          282 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000286 DARBEG010000000
VERSION     DARBEG010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.306"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>282
                     /locus_tag="SCK20_002978"
     CDS             <1..>282
                     /locus_tag="SCK20_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG1146952.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DARBEG010000287          270 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000287 DARBEG010000000
VERSION     DARBEG010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.308"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>270
                     /locus_tag="SCK20_002979"
     CDS             <1..>270
                     /locus_tag="SCK20_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1146953.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DARBEG010000288          239 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000288 DARBEG010000000
VERSION     DARBEG010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.311"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>239
                     /locus_tag="SCK20_002980"
     CDS             <1..>239
                     /locus_tag="SCK20_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1146954.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DARBEG010000289          227 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000289 DARBEG010000000
VERSION     DARBEG010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.312"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="SCK20_002981"
     rRNA            complement(<1..>227)
                     /locus_tag="SCK20_002981"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DARBEG010000290          224 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000290 DARBEG010000000
VERSION     DARBEG010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.314"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>224
                     /locus_tag="SCK20_002982"
     CDS             <1..>224
                     /locus_tag="SCK20_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG1146955.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DARBEG010000291          221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000291 DARBEG010000000
VERSION     DARBEG010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.315"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT           69 a           31 c           41 g           80 t
ORIGIN      
        1 tatactgcaa ggcaagacat ttggtaattg aagaataaca agcaaaaaaa tagcagtcag
       61 gtctcttgac aaagtatagt tgctattctt tttttaggcc ctactttaat gaaatttttg
      121 tttggaggga acgacttcat tcctttgatt atttcagttg aatttaatag agaagcctgg
      181 cgtctattaa gaaatacttt tcttgatgag cgtcaatttt t
//
LOCUS       DARBEG010000292          217 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000292 DARBEG010000000
VERSION     DARBEG010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.316"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT           84 a           41 c           38 g           54 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagt
//
LOCUS       DARBEG010000293          206 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000293 DARBEG010000000
VERSION     DARBEG010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.318"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>206)
                     /locus_tag="SCK20_002983"
     CDS             complement(<1..>206)
                     /locus_tag="SCK20_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146956.1"
                     /translation="IAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEVLL
                     FITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT           56 a           42 c           37 g           71 t
ORIGIN      
        1 caatgctgat attttgcttt aggataaatt tgatggatag tatctttcat gccttttaag
       61 ccgtccgtaa taaaaagcaa gacttcttga actcctctgg agttaatatc ctgtagcagc
      121 tcattccaaa cgtatgttga ttcagttgga gcaatcgcat aactcagtac ttctttagtg
      181 ccgtcttctc gtataccaat ggcaat
//
LOCUS       DARBEG010000294          205 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000294 DARBEG010000000
VERSION     DARBEG010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.denovo.319"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>205
                     /locus_tag="SCK20_002984"
     CDS             <1..>205
                     /locus_tag="SCK20_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146957.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           62 a           49 c           38 g           56 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actct
//
LOCUS       DARBEG010000295        16101 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.060, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000295 DARBEG010000000
VERSION     DARBEG010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.060"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..627
                     /locus_tag="SCK20_002985"
     CDS             <1..627
                     /locus_tag="SCK20_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1146958.1"
                     /translation="EYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHA
                     FRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNG
                     DVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSK
                     QFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            634..1011
                     /locus_tag="SCK20_002986"
     CDS             634..1011
                     /locus_tag="SCK20_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1146959.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1165..1947
                     /locus_tag="SCK20_002987"
     CDS             1165..1947
                     /locus_tag="SCK20_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1146960.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2073..2804)
                     /gene="erm(A)"
                     /locus_tag="SCK20_002988"
     CDS             complement(2073..2804)
                     /gene="erm(A)"
                     /locus_tag="SCK20_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1146961.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(2862..2921)
                     /locus_tag="SCK20_002989"
     CDS             complement(2862..2921)
                     /locus_tag="SCK20_002989"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1146962.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            3313..3975
                     /locus_tag="SCK20_002990"
     CDS             3313..3975
                     /locus_tag="SCK20_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1146963.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            4210..4353
                     /locus_tag="SCK20_002991"
                     /pseudo
     CDS             4210..4353
                     /locus_tag="SCK20_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            4503..4703
                     /locus_tag="SCK20_002992"
     CDS             4503..4703
                     /locus_tag="SCK20_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1146964.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(4957..6636)
                     /locus_tag="SCK20_002993"
     CDS             complement(4957..6636)
                     /locus_tag="SCK20_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1146965.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(6638..6850)
                     /locus_tag="SCK20_002994"
     CDS             complement(6638..6850)
                     /locus_tag="SCK20_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG1146966.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            7243..8973
                     /gene="efrA"
                     /locus_tag="SCK20_002995"
     CDS             7243..8973
                     /gene="efrA"
                     /locus_tag="SCK20_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG1146967.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            8970..10730
                     /gene="efrB"
                     /locus_tag="SCK20_002996"
     CDS             8970..10730
                     /gene="efrB"
                     /locus_tag="SCK20_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG1146968.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            10825..11022
                     /locus_tag="SCK20_002997"
     CDS             10825..11022
                     /locus_tag="SCK20_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146969.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            11175..11660
                     /locus_tag="SCK20_002998"
     CDS             11175..11660
                     /locus_tag="SCK20_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG1146970.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(11764..12357)
                     /gene="clpP"
                     /locus_tag="SCK20_002999"
     CDS             complement(11764..12357)
                     /gene="clpP"
                     /locus_tag="SCK20_002999"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG1146971.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            12537..13064
                     /locus_tag="SCK20_003000"
     CDS             12537..13064
                     /locus_tag="SCK20_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146972.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            13155..13892
                     /locus_tag="SCK20_003001"
     CDS             13155..13892
                     /locus_tag="SCK20_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1146973.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            13896..14813
                     /gene="miaA"
                     /locus_tag="SCK20_003002"
     CDS             13896..14813
                     /gene="miaA"
                     /locus_tag="SCK20_003002"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG1146974.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            14815..16044
                     /gene="hflX"
                     /locus_tag="SCK20_003003"
     CDS             14815..16044
                     /gene="hflX"
                     /locus_tag="SCK20_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG1146975.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5282 a         2680 c         3356 g         4783 t
ORIGIN      
        1 gaatacctct ttccaagaaa agatggatcg ccattaaaac aagaaacatt tagaggtgag
       61 ttaaataaat tagcttatga gcaaaatata gtggataaat caggtgagat ttatagattc
      121 catgcccatg cctttcgcca tacagtagga acaagaatga ttaacaacgg gatgccccag
      181 catattgtgc agaaattttt ggggcatgaa agcccagaaa tgacaagcag atacgctcat
      241 atctttgatg aaactctaaa aaatgaattt actaaatttc aggaaaaact ggttaccaat
      301 aatggagatg tgcttgatct agatgaagat aatgaagtcg atgatgtaga gcttcaatgg
      361 ttcaagaaaa atataaatgc acaagtgctt ccaaatggtt attgtagatt gccagtagta
      421 gcaggtggtt gtccacatgc gaatgcatgc ttagattgca ctcacttctg taccagtaag
      481 caattcttac cacagcacga agaacagtta gagcgtacag aagagttatt agccatagct
      541 aaggataaac aatggcaaag acaagtagag actaatagcc gtgttaaaga gcgtttagaa
      601 caaatcattg gaagtttgac ggggtaatta tcaatggata aacaagttag aaatacaaca
      661 gaaattgtac gtttggcgaa gcagaaatca aaaaagacaa gggaaaaagt agacaaagcg
      721 atttctaaat tttcgattga aggtaaagtt attaatttta attcaatagc aaaggaagct
      781 aatgtttcta aatcatggct ttataaggaa cacgatatta ggcaaagaat cgaatccctt
      841 cgtgagcgtc aaataacagc aaatgtagtc tcaaaaccca agaaaagttc tcgttcggag
      901 gaaatcctta ttaaaacctt aaaaagaaga gtaatggaat tagaaaaaga aaataaaaaa
      961 ttacagaacc aaattcaaaa attatatgga gatctgtata ataaagaata attattaatc
     1021 tgtagacaaa ttgtgaaagg atgtacttaa acgctaacgg tcagctttat tgaacagtca
     1081 tttaggtata tgtccaatct agggtaagta aattgagtat caatataaac tttatatgaa
     1141 cataatcagt tacgaggtga aatcatgagc aatttgatta acggaaaaat accaaatcaa
     1201 gcgattcaaa cattaaaaat cgtaaaagat ttatttggaa gttcaatagt tggagtatat
     1261 ctatttggtt cagcagtaaa tggtggttta cgcattaaca gcgatgtaga tgttctagtc
     1321 gtcgtgaatc atagtttacc tcaattaact cgaaaaaaac taacagaaag actaatgact
     1381 atatcaggaa agattggaaa tacggattct gttagaccac ttgaagttac ggttataaat
     1441 aggagtgaag ttgtcccttg gcaatatcct ccaaaaagag aatttatata cggtgagtgg
     1501 ctcaggggtg aatttgagaa tggacaaatt caggaaccaa gctatgatcc tgatttggct
     1561 attgttttag cacaagcaag aaagaatagt atttctctat ttggtcctga ttcttcaagt
     1621 atacttgtct ccgtaccttt gacagatatt cgaagagcaa ttaaggattc tttgccagaa
     1681 ctaattgagg ggataaaagg tgatgagcgt aatgtaattt taaccctagc tcgaatgtgg
     1741 caaacagtga ctactggtga aattacctcg aaagatgtcg ctgcagaatg ggctatacct
     1801 cttttaccta aagagcatgt aactttactg gatatagcta gaaaaggcta tcggggagag
     1861 tgtgatgata agtgggaagg actatattca aaggtgaaag cactcgttaa gtatatgaaa
     1921 aattctatag aaacttctct caattaggct aattttattg caataacagg tgcttacttt
     1981 taaaactact gatttattga taaatattga acaatttttg ggaagaataa agcgtcctct
     2041 tgtgaaatta gagaacgctt tattacttta atttagtgaa acaatttgta actattgaaa
     2101 atagaaagaa attgttcctt cgatagttta ttaatattag tgacatttgc atgcttcaaa
     2161 gcctgtcgga attggttttt agtgaaaaga acacgatatt cacggtttac ccacttataa
     2221 acaaaagatc gatacttttt gtagtccttc tttgaaatca atggttgatg tcgttcaaga
     2281 acaatcaata cagagtctac acttggctta ggatgaaaat atagtggtgg tacttttttg
     2341 agcattttta tatccatctc caccattaat agtaaaccca aagctcgttg cagattttgc
     2401 aatcttttcg caaatccctt ctcaacgata agatagctat atttagcctg actttcaaag
     2461 gtaattcttt tgacaatatc cgtactgatg ttataaggaa tattaccata tatcttatag
     2521 tttatatgtt ttgggaagga aaattttaga atatccgttt gaatcacttt tatattctca
     2581 gaggggttta ccgcttcttt agtcacttga cataagcctc catcaatttc tatagcagta
     2641 actgatcgac tcattttgac tagctctttg gtaaaatgtc cttttcctga tccgatttct
     2701 attacgttgt cttgtttact gatattcgtg tgattcaata tttcttttac atgcttttta
     2761 gaagtaataa aattttgcgt gtctttaggg tttttctggt tcattataac cttctcctta
     2821 ctggttataa tgaactgaca ttaagcgttc attataacca attaattttg atttggttga
     2881 taacgaactt tattaataac aaatatagaa aaagtaccca tacttttatc tcctttccat
     2941 ttttttcatt atgaatgaga taaagtaatt tctactactg cgatactggt gcacataatt
     3001 gaaacctcct tatgtaactg aatatgattg tatcatcttt tcattttaga ataaaccttt
     3061 atatgtaatt ttgcacttga cacttcagat ggacgttttc ttcaaaataa tatgttttga
     3121 aacttgataa gagaagccac tgtctttact aaaaagtaaa ggctgcgata aaggacagtg
     3181 actttaataa agttgcactg ttgtattgtc tttggataaa attgtctaaa gggaagtttt
     3241 atccccaacg aaaaaacatc taaagtggta agtaatgaaa gaatgtcgat aaagtaggaa
     3301 tgggggatct ttttgaatac aatagaatca cagtttgata aggttgcaga agattacgat
     3361 ttcgtgaatg agcttttgaa tgattattca ttctttgtgt ctaatatgtc tccaaagaaa
     3421 ggcagagcat tagatatcgg atgtggctcg ggtttgttag tggagaaatt agcaagttat
     3481 tatgatgaag tggtagggat tgatatttct aatcaaatgc tcgatcttgc caaatctaaa
     3541 cgtcaactaa caaatacggt ctatctgaat atgaatgcag aacaacttaa ttttaatgag
     3601 aagtttgatt ttattgtaag ccgaacaacc tttcatcatt tggatgatat agccagcgta
     3661 atacagcaaa tgaaggaact gttgaatgag gaaggaagaa tagttattct tgataatgta
     3721 tctgaagtcg agacaccgcc tacctatgtt tataaattag gggcaatcca agagttttta
     3781 ccgcattgtt tcaaatttgg aataaaaaat gcgattagaa tttataatca caacacatct
     3841 aaatcatggc ttgagcattt agcttcagat aagtacctat ctgaacaaaa ttattacgat
     3901 ttatatgaaa agttgttgcc tggatgccaa ttccataaaa tgggttgggc aatgggggtt
     3961 gtctggacaa aataacttaa agctgtggct ttgaaaagtt gaatttgtga ttgttttcag
     4021 agctatcggg tgctttagta taaataacaa tgaataaaaa atcagcctgt atcttatgtt
     4081 actattaagt aatttaagat acaggtttta ctatttttga aataagtacc ttccacttca
     4141 aatacatgta ttcaaagtga aagtattgat ttaaatgatt cttttgtaat ttactcttga
     4201 taatatgaaa ttccatcgca gcaggatatg aattttacca aaaggataca aaagtgtgga
     4261 gaaatgatgg ggatcactgt actggaccac ttgatcatcg gacgcaaacg atattttagt
     4321 ttgagagaag aaggaatgat ggaagagaag taagttgctt tcttgcaaaa aaaatgaagc
     4381 aagtgtacaa ttatattgta attttgtttg ggaatagaag ttgaaacatc tgttgcacct
     4441 ctatcgtaca cgagcaatgt tacaatttaa attagtgctt ttggcacgag gaggatatta
     4501 ttatggaaaa cggaacagta aaatggttca acgctgaaaa aggatttgga tttatctcac
     4561 gtgaagatgg aagcgacgta ttcgttcact tctcagctat ccaaggtgac ggattcaaaa
     4621 cattagaaga aggtcaagca gtgactttcg acgttgaaga atcagaccgc ggcccacaag
     4681 ctgcaaacgt tgtaaaagct taattttatc tgtaaatcaa attgatggta gacatcaatt
     4741 tttacgagag aacaaagcga gtaacaaaaa tacctgccac aagagacaat ttctcttgtg
     4801 gcaggtattt ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa gagccacctt
     4861 gtatcaagga ataagaacaa acaaaaatgg cttctcatgt ttttcatttg ttttctctta
     4921 tattcaggac aaggtgctga tttcataacc tcttatttat tcttcatctg gtgtcattac
     4981 cattggcaaa atcattggat gtctttctgt acgttcgaat aagaaaggct gaagcgcgcc
     5041 agtcatcgct tctttcaatt tatattctga acagtcaggt ccattcaaac attctcttaa
     5101 agcgtggaat aagattcgct gagcttcatt gatcatttct cctgattctc gcatgtagac
     5161 aaatccacga gagagaatat ctggaccagc caagatttct tttttcttga tatccactgt
     5221 cgcgacagct aatacaagac cttcttctga aagcaggtga cggtcacgta aaacgacatt
     5281 accaatatca ccgatcccgc taccatcgat atacacatct ccagccacaa aatgcccagc
     5341 aggacgcgca gaatcgctag ttaacgctaa aacatcgcca ttttccatga tgaagcagtt
     5401 ttctgcagga acacctacat cttgtgctag agaagcatgt actttcaaca tgcggaattc
     5461 tccgtggaca ggaataaaat attttggctt catcaaacga agcattaatt tttgttcttc
     5521 ttgtccaccg tgaccggaag tatggatgtt attgattttt ccgtgaatca cttctgcacc
     5581 agcttcagac agcaaattga tcaaatgatt gacacttgtt gtattaccag gaataggtga
     5641 acttgaaaag atcactgtat cgtctggctg gatttggatt tgacggtgag taccattcgc
     5701 gatgcggctc aacgcggcca tcgcttctcc ttgagaacct gtacatagga tcatcgtttc
     5761 attggcaggc aagcgattga tttcgtgtgc atctacaaat gtgccgtctg gaactttgat
     5821 atatcctaaa cgttgaccgt tgacgatggc attttccatg cttcgtccga aaacagcgat
     5881 tttacgacca gttgccacag cggcatctgc tgcttgctgc aaacggaaga tgttagaagc
     5941 aaagctggca aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa tagaatgccc
     6001 aatcgttttt tctgatttag taaatgttgg aatctcagca tttgtactat cagataaaag
     6061 gcacaagact ccttcttccc cgatacgagc cattttatgc aagtttgctg gttcgccaac
     6121 aggagtgaaa tcgaatttga agtctccagt cgctacgata tttccagaag gtgttttcac
     6181 cacgatccct aatgcatctg ggatactatg ggtcgttctg aaaaagctga cagaagtctt
     6241 tctaaaacgg atgatcgtat cttcattgat ctcatggagc tctgcatctc gaagcaagcc
     6301 atgctcatct aatttgtttg tgatcaacgc taatgcgaga ggaccagcat agataggaat
     6361 atttgcttgt ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc cgtgagtaat
     6421 caccaaggct tttactttgt gtaagttttg tatgatgtag ctgtagtcgg gaatgacata
     6481 gtcaattcca agtaaatcgt cttccgggaa tttgatcccg gcatcaatca aaatgatttc
     6541 atcttggaat tgtaccccat acgtattttt cccgatttca cctaatccac caatagcaaa
     6601 gacgcctgtc tcgttatttt tgatggaaag tttcatatta aaactccact aatgagaaat
     6661 cagcattttc tttttcgtac tctagatgac ttccttctag aagttgaacg aattcaatat
     6721 tgtatggggt attttcttcc acacgttggc gagcgtctac atcgcttttt gcctcaatgt
     6781 aaagagattt tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga taataaactt
     6841 tatagatcat tgttttgtct cctttatctg cgtaaagcag tgatttagct agttgatttt
     6901 tttagacaac acaaaacgag gggacaagcc acctggtttt tcatatgctt tgactagaat
     6961 actggattcg agtcaaagag acaagcctcg tttttgctta tctgttcgga tattttttca
     7021 accgtacctt ctgtataaaa aatatacaac aattaattgt tattctagca taaaacagca
     7081 aataagtata gaaatcaact agattgaaag aaaaaatcaa tgtttatcat cccgctataa
     7141 tcataatgac ttttttatga gagaagtgtt ctattaatag gctcgttgtg ctatcatttt
     7201 tagtaatgaa aatgtaacaa aagattaaaa ggagatgaga aagtgaaact aatgtggcat
     7261 tataccatgc gttataagaa attcttgttt ttcaatttca tatgcgtatt tggttttatt
     7321 ttgattgaat taggacttcc tacgatttta gcacgtatga tcgatgtagg aatccgcaat
     7381 gatgattttg attatgtgaa gcaacaaggg atatttatga tcgttattac tgtaatcggt
     7441 attattttga atatcttgtt aggttatttt ggttcgagga ttacgactaa tatcgtagca
     7501 gatattcgag atgatttgtt cgaaaagatc caaagttatt cgcatcagga atacgaaaat
     7561 ctaggcgtat cttcattgat cactcgtaca acaaacgatg cttatcagat catgttgttc
     7621 ctgcaaaata ttttacgtat cggttttatg gcaccaatga tgttcattgt cagtttgtac
     7681 atggtcatgc gtacaagtgt gacactcagt ttgtatgtag taggtgcatt acctttacta
     7741 ttattggcag tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa gcaacaaaaa
     7801 aacttagata aaatcaacag tatcttgcgg gaaaatcttt caggattgcg tgtgattcgt
     7861 gcattcgtca atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga gaattatgct
     7921 tctagttcaa aaagcttatt ccgtttgatg gctgttgcac aaccgggttt ttatttctta
     7981 tttaatatcg taatggtatt gatcatctgg agcggggcag tgcaaatcga tgcaggcgag
     8041 cttcgtgtag gagatttgat tgcgtttatc gagtatatat tccatgcctt gttctcattc
     8101 atgttatttg ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc tggacgtatc
     8161 caagaagccc tagatgccaa accttcgatt gtagaagatc caaatggtat atctgataca
     8221 aaaactaaag ggtatatcga atttcaaaat gtaacatttg cttatccagg ccattcgcaa
     8281 gagccagtga tacgaaatgt cagttttaca gcctctccag gagagaccgt tgcttttatt
     8341 ggaagtaccg gtagcggaaa atctacgttg atccaattga tccctcgctt ttatgatgtg
     8401 agtgaaggaa agatcttgat cgatggagta gacgtgagag attatcggct aagcaagttg
     8461 cgagataaga ttgggtttat tccgcaaaaa gcgctgctat ttactggaac gattgcagat
     8521 aatttgcgat acgggaaaga agatgctacg caagaagaaa tggaacgtgc agctgagatt
     8581 gctcaagctt cagaattcat ttctcgaaaa ccagatggat ttaatgaaca tttatcagaa
     8641 ggtggttcca atttttcagg tggacaaaaa caacgattgg caatagccag agccatcata
     8701 cgccgtccgg aaatctatat ttttgatgac agcttttctg cattagatta tcaaacggat
     8761 gcgaagttac gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt gatcgtagca
     8821 caacgtgtgg gaacgatcat gcatgcagat aagatcatcg tattaaatga aggagaagtc
     8881 gtcggtatgg ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta tgatattgcc
     8941 gcttctcaat tatcaaagga ggaattagca tgaaaaaaac cttttcttcc ttaaaacgtt
     9001 tagctcgata tatcagaccg tatcgtgaga cttttctttt agtgatccta tttacagttc
     9061 tgacagtggc tttcaacgtg gctttgccat atgttacagg acttcctact acagaaatca
     9121 gccgtaatat tgccaatggc gaatcaatca atttttctta tatattcaaa tgtctcattt
     9181 ggatcacagt tgttggaata gggtattgta tttcgcaatt actttctggt gttttgatga
     9241 caaatgtcgt tcaaagtgcc atgcatgatt tgagaagaga catagaagaa aaaatcaatc
     9301 gtcttccggt ttcatatttc gataaaaacc agcaaggaaa tattttgtct cgtgtgacga
     9361 atgatgtaga tgcagtcagc ggtgctttac aacaagcttt tattggtatc gtaaatgcga
     9421 tattgggaat tacgttggct gcagcaatga tgttttatat ccagccgatg atggcgctga
     9481 tctctatgat catgattcca acatcgatct ggatctcaaa acgagtgatc aatgcttcgc
     9541 agaaatattt tcaaagcatg cagaattctc ttggtgagct aaacggctac gtccaagaaa
     9601 atatgacagg ttttagtgta ttgaaagtat atggacgcga aaaagaaaca ttggaaggat
     9661 tcaaaaaagt taatcacagc ttgaaatact ttgggtttcg tgcagcattc atttctggct
     9721 tgatgatgcc tctagttcaa ttaactgcct atgctactta tattggtatg gctgttctag
     9781 gaagctttta tgttattact ggggcgatcg ttgtcggtca actacaagca ttcatccaat
     9841 atatttggca aatcagtcag ccaatgggga acgtgacaca attgtcttca gttttgcaaa
     9901 gtgcatcagc ggcaacgaaa cgtgtattcg agattttaga tgagccagaa gaaaaagaaa
     9961 acaaagtaga tgcttcactg ccagaaacga ttttaggaga tgtgacattc gatcatgtcg
    10021 attttgcata tgatccgaag aaaccattaa tccaagattt gaattttgaa gtgaaagctg
    10081 gtcaaaccgt agctgtggtc ggtccgaccg gtgccggcaa gacgacattg atcaatctat
    10141 tgatgcgttt ttatgacgta gacaaaggag caataaggat tgatggaatc gacaccaaat
    10201 caatgagtcg cagtgatgtg cgttcgctat ttggtatggt cttgcaagat gcatggcttt
    10261 atcaaggaac gattgcagat aatatccgct tcggaaagct tgatgcgaca gattatgaag
    10321 tagttgatgc tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg ccagatggat
    10381 atgatatggc catcaattct gagggagaca atgtgtcttt aggacaaaaa cagttgctga
    10441 cgattgcccg agcagtggtc tccgatccta aaatcttgat tttagatgaa gcaacaagtt
    10501 cagtagacac ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg atggagggac
    10561 ggacaagttt cgtcatcgct caccgtttat cgacaatccg tgatgcagat ctgattctag
    10621 tgatggatca aggtcagatc attgaaaaag gaacgcatga gagtttattg gcacaaggtg
    10681 gattttatga aaaactgtat aacagccaat ttgctgagga agcagaataa gtcatataag
    10741 aataaaacag tttaaaactt attttttgtt ctctattctt gataatggta aagtaaagtt
    10801 atcaagaaaa gtgaggacac ggagatggaa cagtttttaa atgaaaaaat tactgaagcc
    10861 aaaagagaag ttgatcgttt aacgcacgtc tgtcagacga agccttttga ttcagctgat
    10921 ttttttgaaa atgagtttca aatcgaacgc ttggcagcac aaatagaagt gtacgaatta
    10981 attttaaaaa aagtaataga agaaaatata gatgtacaat aaaaacaggt aaaacgtcag
    11041 ttaccagata ctgtaaatag acatctggcc tgagcaggtt ttacctgttt tttttcttat
    11101 gaaataaata tatgctatga tacctttatt caataggatt agtttctaca aaaaatgaaa
    11161 ggagaaaaat aaatatggaa cggtttatag catcaaatgc cacagtgatt ggtgatgtga
    11221 cattgtcaga agatgtgacg atctggtatc aagctgtttt acgcggggac agtaattgga
    11281 taaaaatagg acaaagaacg aatatccaag atggtacaat catccatgtc gatcatgatg
    11341 cacctgtaga tattgcggag aatgtgacgg taggacatca gtgcatgcta catggctgta
    11401 caatcgaaaa aggggcgctg atcgggatgg ggaccacgat attgaatcat gcggttattg
    11461 gtgagaatag tttgattggt gctggatcat tagtaacaga aggaaaagtt attcctccta
    11521 atgtcttggc ttttggtcgc ccagctaaag tgatccgtcc tttgacaaaa gaagaaatac
    11581 aaaaaaacaa agaaaacatc cagcattatg tcgagatagg tcggatgcat gcgcaaggaa
    11641 aattcaagga gcaaagatag gcagtagcat ccataaaata aaaaaggatg gtcagtttga
    11701 gttgaccatc cttttttatt tggtttagat aagagagctt aaaaaagctt cctttgaaac
    11761 agattaattt aatgaagaac tgttttccat gacttcatcg atcaatccat atgcttttgc
    11821 ttgttcagct gtcatatagt tgtcacgatc tgtatctttt tcaatcactt caagcggttg
    11881 tccagtgcgt tcagctaaaa ttttgtttaa gcgttcgcgt gtttgcaaaa tatggcgtgc
    11941 cgcaatttcg atttcagttg cttgcccttg agcgccgcca agaggttgat ggatcatgat
    12001 ctcagcattt ggtaaagcaa atcgtttacc ttttgtacca gctgtcagca agaagcttcc
    12061 cattgaagca gccataccca taacaattgt ttggacatct gctttgacaa agttcattgt
    12121 gtcgtaaata gccatacctg ctgtaactga accaccagga gaattgatat aaagatagat
    12181 atctttttca gaatcttgag cgtctaagaa aagcaattgg gcgatgatcg aattagcaag
    12241 atcatctgtt acttggccgc ttaacatgat gatacggtct ttcaataaac gtgagtagat
    12301 atcgtaagct ctttcacctc gtgaagattg ttcaatgact gtaggaatta aattcataat
    12361 agagttcctc cttgatttcg tttagaaata gtgtaccata atttagtttc taatacctaa
    12421 aatgtagtat acatttttgg tcaaaaaagg tcaaataaaa aacttaggca aattagtcgc
    12481 ataaacccgc tttatggtat gataagcaga gaaacaaaac aaggaggaac aacagattga
    12541 tagttggtat tactggcctt atcggagtgc tgacaggtgc tgtagttgtc tatatcggca
    12601 tgatactgca aatgcgtcac ttggaaaata aagaacagcg tagacgtgaa ttggaattga
    12661 aagtcaaaga aatcgagacg ctgaatcaat taaataaaaa gatcaacgag atcttgcaaa
    12721 aacggacaac gctgatggaa gaatatgtca gctttgatgc atttgatgat tgttatatca
    12781 cgattgacga ttttgcgtat ctccaatcat tttcagcgca aaatagtttt tatctgccaa
    12841 attatatttt agacgagcta tttaagaata tctcccatcg aaaagtgatt ttatctccag
    12901 atgaaacaat gaagattggt ggatatgctt ataaaggcgg acgcgtgatt ttagagaatt
    12961 ttacggatag tctgactgaa atgatcaatg aaaaaaaagc tcagttgaag actatgacgc
    13021 gccagccgat tggtttcttc tcgaaagatg atgtttggag ctgatatttt gaagcattgc
    13081 cgttgcacat gagagcgtgg taatgctttc tttttttctt aatgatacaa tagaactgaa
    13141 cggagtgaag aaagatgtta caaatctatg cccatcgcgg aagcagcggc acccacccag
    13201 aaaatacgct tcctgcattt gcagaagctg tacgagtagg tgcagatggt atcgaactag
    13261 atgtccatct aagcaaagat ggttatttga tcgttatgca cgacgaagaa gtggaccgga
    13321 cgactaacgg taaagggctg attcgagaaa aaacattgga agaactcaaa aaattgaatg
    13381 cagggggcaa attcagcaaa gaatttccag cagctaaagt tcctgaattg agagaagtgt
    13441 taggactgct cctccaaaaa aattaccgag ggatgctaat gattgaaatt aaaactgatc
    13501 agtatgaata tcccggtatc gaagaaaaat tagccgaaac attaaaaagt agagattggc
    13561 cttttcaaca tgcatattgc agctttcatt tggattcttt ggaaaaaatc gcattactgg
    13621 aaccggagat acagctggac ttattgatga gtacttctga taaaaagatc caactagcag
    13681 cagaaacccc ttatatagaa ggagtacatc caaaaaatac ttggttgttc gaacatccag
    13741 tccaggcagc tgattttcca aaagcaattc gtgcatggac gattaacgat gaagaagaaa
    13801 tggccaaatg tcaagaactc aaaatagatg gaataataac tgattttccg gagaaagctc
    13861 ttcggtggaa acaatcaacg aaaagtaggt aaaatatgga aaaagtatta gtgatcgtcg
    13921 gtccgacagc agtcggtaaa acagcgctaa gtgtggaact cgcacaacta tttggaggag
    13981 agatcatcag tggtgattca ttgcaaatct ataaaaatct ggatatcggt acagcaaaaa
    14041 tcaaagaaga agagatgaat aatgttccgc atcatttgat tgatgtgatc gaacctactc
    14101 aacgttactc agccgctgat tttcagcatt caggtcgaca attgattacc gagattgcga
    14161 accgagggca cctacctatc atagctggtg gtacgggtct ctatatccaa tcccttctat
    14221 atgattatca gttaggagcg acagaagaag cgaatcctga gattagaaaa aagtatgaaa
    14281 cgttagctga aacaattggt tcccaagcat tatggaatca gttgaaagaa aaagacccgg
    14341 cagctgctga gaaaatccac tggaacaatc aacgcaaagt gataagagct ctggaagttt
    14401 tagagacgac aggacatagt atcacggcac ctaaagaaga gccaaagcaa ttgtatgatt
    14461 acctcatgat tggattagat acgaagcgta gtattcttta tgaacgaatc aatcaaagag
    14521 tcgatttgat gctagcagaa ggtttagtag cagaagcgcg taaggtgtat gagcttggag
    14581 aagtccaggc cagtcaaggt attggatata aagaattttt cccttatttt gaagggaaag
    14641 aaacattgga agaagcaaca gaacaagtca aactacattc gagaagatac gcgaaacgtc
    14701 agttgacgtg gtttcgtaac cgtttggatg cacagtggtt tgatcttata agtgagccaa
    14761 atcaaaaaag tcagattgag caaacaatca aaaaatggct ggaggcggaa taaaatggaa
    14821 gaaaaagtga tcatcgtagg ggtagaaacc gaagcaaacc agcgttactt ttcagagtca
    14881 atggaagagc tagttcagct gacgaatacc gcatcaggag aagtcgtatt cacaattaca
    14941 caaaagagac cacaagtgga tcggcaaacg attatcggta aagggaaatt acaagaatta
    15001 gttcaacaag cggatgcgca tgaagcggat ttgatcatct ttaatcatga gctaactccg
    15061 agacagagcc aactgattac cgatgctgtt ggtcttccgg taattgatcg cgtgcagctt
    15121 atcttagata tttttgcgat gcgtgctcgt tcgaaagaag ggaagctgca ggttgaactt
    15181 gctcagttag aatacctgct gcctcgttta gtagggcaag ggaaaacatt atctcgtctt
    15241 ggcggtggga tcggaacaag agggccaggt gaaacaaagt tagaaacaga ccgccgacat
    15301 atacggaata aaatttttgc tgttaagaaa gaattgaaag aagtagaagc tcatcgagag
    15361 cgcaatcgcc aaaaaagaaa aaatagcgag atttttcaga ttggacttat tggctacacg
    15421 aatgccggga agtcaactat cttgaatcta ttgacacaag cggatactta ttcgaaagat
    15481 caattgtttg caacgttaga tccattgact aagagatggc gttttgctga aggatttgag
    15541 attactgtaa cggataccgt cgggttcatc caagacctgc ctacgcaact gatcgatgct
    15601 ttccattcta cattagaaga aagtcaaaat atggatcttt tgctacatgt agtcgatgct
    15661 agttctccag atcgtatttt acaggagcag actgtcttaa aattgatgga tgaactgaat
    15721 atgaaagaaa tgccgatact gactgtttac aataaggcag accagatcga cccagcgatg
    15781 ttcacgccta cgctattccc aaatgtattg atttctactc agacgaaaga aggtaagatg
    15841 gcgttgatcg aagcaatcaa acgtcagctg atggaactaa tgacccctta tacactttct
    15901 gtgccaagca acgaaggaca gaaattaagc gagttgagaa gacaaacaat ggttttggac
    15961 gaagcgtaca tggaagaaac aaacgaatat gaagttaaag ggtttgcaaa aaccaattca
    16021 aaatggttaa gaaaaaacca ataaaatttt atttttttaa caatattatt tgtcaaatta
    16081 agcgagacaa aactttccat t
//
LOCUS       DARBEG010000296         7959 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.106, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000296 DARBEG010000000
VERSION     DARBEG010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.106"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..1564
                     /locus_tag="SCK20_003004"
     CDS             128..1564
                     /locus_tag="SCK20_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1146976.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="SCK20_003005"
     CDS             1753..4200
                     /locus_tag="SCK20_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1146977.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="SCK20_003006"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="SCK20_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1146978.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="SCK20_003007"
     CDS             complement(5690..6793)
                     /locus_tag="SCK20_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1146979.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="SCK20_003008"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="SCK20_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1146980.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2665 a         1359 c         1729 g         2206 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DARBEG010000297         7108 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.112, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000297 DARBEG010000000
VERSION     DARBEG010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.112"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            44..892
                     /locus_tag="SCK20_003009"
     CDS             44..892
                     /locus_tag="SCK20_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1146981.1"
                     /translation="MIKVGTHKKLTFVLWVLLIGSVGFGIYKNFTAIDTHTVRETEII
                     KQQIVDTNQVESFVKSFAKDYFSWQQSQEAIDKRNEKLTHYLTEELQVLNEEMIRKDI
                     PTSSSVNDIQVWQVSQVNENTFEVLFSVEQVITEDKDKETISSSFHVVVHIDESDNMV
                     IIKNPTMSKKPQKSDYQPKQLESDHTVDTETMDEITSFLETFFQLYPTATEKELTYYV
                     SNHVLPMINKEYVFEELVNPIFTRKDNQVIVNVAVKYLDQETKATQISQFELILEKQD
                     NWKIVK"
     gene            1137..1253
                     /locus_tag="SCK20_003010"
     CDS             1137..1253
                     /locus_tag="SCK20_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1146982.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            1269..2072
                     /locus_tag="SCK20_003011"
     CDS             1269..2072
                     /locus_tag="SCK20_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1146983.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            2307..2543
                     /locus_tag="SCK20_003012"
     CDS             2307..2543
                     /locus_tag="SCK20_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1146984.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            2550..4502
                     /locus_tag="SCK20_003013"
     CDS             2550..4502
                     /locus_tag="SCK20_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1146985.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(4574..5071)
                     /gene="dfrG"
                     /locus_tag="SCK20_003014"
     CDS             complement(4574..5071)
                     /gene="dfrG"
                     /locus_tag="SCK20_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1146986.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            5433..6473
                     /locus_tag="SCK20_003015"
     CDS             5433..6473
                     /locus_tag="SCK20_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1146987.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            6571..6759
                     /locus_tag="SCK20_003016"
     CDS             6571..6759
                     /locus_tag="SCK20_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1146988.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(6817..>7108)
                     /locus_tag="SCK20_003017"
     CDS             complement(6817..>7108)
                     /locus_tag="SCK20_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1146989.1"
                     /translation="MKRGLTREQVGTMIEIDPRYLTNIENKGQHPSTQVLYDLVSLLH
                     VSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT         2484 a         1130 c         1290 g         2204 t
ORIGIN      
        1 accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta aagttggcac
       61 gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg gatttggaat
      121 ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa tcattaaaca
      181 gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga aagactactt
      241 ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga cccattattt
      301 gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc ctactagttc
      361 tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca cctttgaggt
      421 actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta tatcgtctag
      481 ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca aaaatccgac
      541 aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa gtgaccatac
      601 agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct tccagctcta
      661 tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt tacctatgat
      721 taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa aagataacca
      781 agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa cccaaatctc
      841 gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat aacaaatatt
      901 ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa ttggagattc
      961 ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg agttaataaa
     1021 tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa ataattagat
     1081 gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt tatcatgtga
     1141 ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg cataaaaatc
     1201 ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt tgaatggagg
     1261 aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca ggaaaaacta
     1321 ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga agcgtggaca
     1381 aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt acaattcaga
     1441 caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac acgccaggac
     1501 atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg gcaattctac
     1561 tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat gcacttagga
     1621 aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga attgatttat
     1681 caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc aaacagaagg
     1741 tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa tgggatacgg
     1801 taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca ttagaagcat
     1861 tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc cctctttatc
     1921 atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt actaataaat
     1981 tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc aaaattgaat
     2041 atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt acaaaaagtg
     2101 aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt tttttattgt
     2161 taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt atataaaaat
     2221 gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg ttacaattta
     2281 ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat aagaacgcta
     2341 agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa tacgctgaat
     2401 atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt ttagacgata
     2461 agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg
     2521 gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta gccacgaagg
     2581 aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa gatagggcta
     2641 aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa atgttgttct
     2701 tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat tactgcatca
     2761 atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta aaagggaaac
     2821 caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt atgtactgca
     2881 atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct ttatctaatt
     2941 ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa gatgttgagc
     3001 cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca ttcaatgagc
     3061 cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat caatgtaagg
     3121 cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact tatcaccaac
     3181 aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt tcagtcagtc
     3241 gtacttgtga tattttagga attggagttg gaacctacta tcataagctg gagtggctgt
     3301 ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa acaaagaaat
     3361 ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac gtccgtaaga
     3421 aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa acgtatatag
     3481 ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc tatgattgga
     3541 atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac catctcaata
     3601 cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa ccatccgcta
     3661 acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact aataggagta
     3721 gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac tattggttaa
     3781 ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac ttctctatta
     3841 gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca catcatttta
     3901 tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa
     3961 aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt ctacggactt
     4021 tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa gaggctatta
     4081 gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat ccattagcca
     4141 ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct ctggaaccaa
     4201 acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc tttattcaac
     4261 agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt ggtgataaga
     4321 agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt acaattctaa
     4381 gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt ttgacacctg
     4441 ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt tatttcaaat
     4501 gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg ttcttcttca
     4561 attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa tagtagttat
     4621 acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat tcctcataat
     4681 tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg atgtacattt
     4741 tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata aaaatttctt
     4801 cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca caaccattaa
     4861 aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag gctcttccga
     4921 ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta tttttaacat
     4981 attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc actctattct
     5041 tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta actaatttac
     5101 catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg ttagttgaat
     5161 aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa ttttcattca
     5221 attatcattt tcaattgcat tttataaatc attttgtatg taatttggta tgtaagttaa
     5281 aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca aaactgaact
     5341 acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat tcggttagag
     5401 tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat ggtgaattat
     5461 gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag tttttgaagt
     5521 taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt gaaaatccgc
     5581 accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa atgttgcttg
     5641 atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg gattctacga
     5701 cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt agtgcactgt
     5761 tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt tatatggaga
     5821 gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat cctttctggg
     5881 cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag tatgagagct
     5941 cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa gggatacgct
     6001 atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc tgttttaagt
     6061 acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt actcctattg
     6121 tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat cttagtttta
     6181 aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc aaatattgtg
     6241 caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga gaaattcctg
     6301 ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg cttagtgttt
     6361 gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc cagacccgtc
     6421 gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact tagtgcgttt
     6481 tatgttgtta tataaatatg gtttcttatt aaataagatg aaatattctt taatatagat
     6541 ttgaattaaa gtggaaagga ggagattgtt attataaact acaagtggat attgtgtcct
     6601 atttgtggaa ataaaacaag actacgaata cgagtggata ctatacttaa aaatttccct
     6661 ttatactgcc ccaaatgtaa gaacgaaact ttaattaatg ttcaaaaaat gaatataata
     6721 acaatcaaag agccagacgc caagacgcag agccgataat ttgagaaatg aaactctcat
     6781 cttatcggct ctttttgttt atctgaattt tactgactag ccttcaatat ttcttgcttc
     6841 attgatacct tttgctagtg cttccattaa ggatagttct ttgtctgtaa agctatccat
     6901 gtatttttct acctgtaacc ttcgagtgct tttaatcaaa ttatctgtag gtaagaaaaa
     6961 ttcatcaata gatacatgga gtaatgatac aagatcatac agcacttgtg tacttgggtg
     7021 ttgtcctttg ttttctatat ttgttaaata acgtgggtca atttcaatca ttgttcccac
     7081 ttgttcacga gtgagacccc gtttcatt
//
LOCUS       DARBEG010000298         5880 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.123, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000298 DARBEG010000000
VERSION     DARBEG010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.123"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..1122
                     /locus_tag="SCK20_003018"
     CDS             226..1122
                     /locus_tag="SCK20_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1146990.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1272..2213)
                     /locus_tag="SCK20_003019"
     CDS             complement(1272..2213)
                     /locus_tag="SCK20_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1146991.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2339..3460
                     /locus_tag="SCK20_003020"
     CDS             2339..3460
                     /locus_tag="SCK20_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1146992.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3718..5220
                     /gene="eat(A)"
                     /locus_tag="SCK20_003021"
     CDS             3718..5220
                     /gene="eat(A)"
                     /locus_tag="SCK20_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1146993.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5248..5814
                     /locus_tag="SCK20_003022"
     CDS             5248..5814
                     /locus_tag="SCK20_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1146994.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         1936 a         1036 c         1228 g         1680 t
ORIGIN      
        1 agaataaata gcaatattta acaatgacta atgataacaa attttcagac aattacaact
       61 attcagaaaa cttttaggcg aattttaata gaagaatgat ttatttcatc atattttcat
      121 acaaatgtta aattttcata caaatatagg aaacgtttta ctggcttttt actatttaaa
      181 caggctatca taggaaaaag tgacagagaa agtgaggcgg aaaagatgaa caaaaatgac
      241 gtactgctag ttgcaaaaaa taaatcactt tattttttgc cttccatccg ctctttagat
      301 gtttggtccg gaaaagattt actacagtcg gaattcatta aatcagctag acaacagttg
      361 gcaaattatc agctgatcgt ttttttagat tatggatttg atatggagat ggctcgacgc
      421 gtgcgcccat ttaccaatgc aaaaattgtg ttattttttt ggaatcactt caaagcagaa
      481 cacgaggcga tgctgaagca ggcacaagat gaaccggcaa tcgatgagat ttatcatttt
      541 gattttttgg aagcaaaaga acttgggctg aaacataata gttcatttta ttcgaaacat
      601 atgaattttt cttctagcga accaaagacg gatctatttt ttggtgcaac gaataatggg
      661 agaaaagaac gagcagaaag ctataagcag gaatttataa aaagagatgt tacgaccaac
      721 tatttcattc ttccacatcg agggaatgaa caacctggat atcttactta tgaagagtac
      781 ctgaaaaaaa caggggaatc gcgcgggatt ctggaactgt tgcgggaggg tcagcaagga
      841 gtgactttgc ggacatttga atccatgtat tttcaaaaga aactagtgac ggataatcaa
      901 gcgattgctc attatcattt ttatcgtcct gaaaatattt ttttgcttcg agaacgtgat
      961 ttagatgaat taccggaatt tctgcataca ccatttaagc caatcctatc agagctattg
     1021 gaattttatg atgcagaaaa ttgggcaaaa cgatttatca ctaacgattc cactatttat
     1081 gagcaatacg aatatgacct ctcctctgtt gaaaacaaat aaaaaaccta cattttgaag
     1141 agattatgta ctgaaacaaa caagcacaat tcttatggca tcatgagagt tgtgttttct
     1201 ttttgatctt ttattgggct taaaaaaaga gaaccagctg ctgattcagc tgattctcat
     1261 cagagagcct tttattctgc gccaaggatt ccttcttttg catggactaa ttgggtcaaa
     1321 aaggcacaat atggtgtagc aatatcatat ttttgtcctt ttcgccaaat ggcaccattg
     1381 atgtagtcga tttcagtcaa acgatggttt ttgatcaggt cttggtacat agatgggtaa
     1441 tgcaacccga tgttgtcagg atcataagta gaagcaatat gttggtacac ttcttcttga
     1501 tctagtatga ttccttcttt tgctgcgata gaagcaaatt cagatacgat tgttcgaacc
     1561 atgctctctg ccgctttttg cgcgcctagt tcagcaatat tacaatccaa aatcgtgcat
     1621 aaaccattca atgttccatt tacacaagct tttcgccaaa tggaataacg aacgttatgg
     1681 ctatagatcg gatttaaatt tgcttcttga aagacttcta caactttttt tgtaaatgct
     1741 tcgccttctg gttccaagtt ctctagttcg atttctccat cacctaacaa tgtgactttt
     1801 ccgggaccag ctagaccagc tgtccacatc gtgatcccaa ataagatatt tttctttggc
     1861 acatattttt ccagtacatc ttcatgacct aatccattta gtaaacacaa aacataagta
     1921 ttgtcagtaa taatggattg gatcgaacaa aacatatcat ctaattgatt ggcttttgtt
     1981 aatgctacga tcaagtccac gtgttcgttg ctttcgatga tttcttctgg tgaatagatt
     2041 ggcagttttg cgaccacttc tttaccgtta aagtcagcaa tcaacccatt tgtacgaatg
     2101 gcttcaatgt gagcaggcca acgatcgatc aagaggacct catttccgct ttgatgtagc
     2161 atcaatccga ttcggctgcc cattgctccg gccccagcta ttgcgatttt catgtttttt
     2221 ctctccttta ttcaatgagt tcccttttag tatatagaaa aatggaagaa aatagcattg
     2281 ttttgtttga aaaaaatgaa aaaaagtgtc ttacttaaga agaaagtgag ggatacagat
     2341 ggatattttg gcggcaattg attcatttaa aggaagtgct tcttcatttg aactaaatca
     2401 ggcagttcta aatggctggg aagatcgtaa aggaagaaaa atcaatgtgc cgattgcaga
     2461 tggcggagaa aatactgcag aagcaatcta ttacgcgctc aggggaacat ggcgtaccat
     2521 tgatgggatg gatctcttat tccgtcagcg aaaaatcagc tatttattga caacttggca
     2581 aggaaaaaac accgcagtca tcgaatctgc acaagcattg gggttagatc tacttgataa
     2641 accaacggat caaactatcc gacttgcttc cagttatgcc ttaggagaac ttattaaaga
     2701 tgcactgctt caagaagtag aacaaattat tgtcacttta ggaggaagcg ggtgtagcga
     2761 tggtggtcta ggattactcc aatccttggg tgcttcttta gaagggtaca cagaaggaaa
     2821 tcctttatta tctactaaaa aacttcattt tgatcgaaga aacgttcttg tttctaaagt
     2881 aaaattgatt atcgctgcag atgttaccaa tccatataca ggtcaacaag gagctgcttg
     2941 gcaatttgga agacaaaaag gaggaataga tacgacgcta gctttcttag ataaacaagc
     3001 aggaaagatt gctcagcaaa taaaagattt ctgggaggtg gatttagatg atattccagg
     3061 aagtggagca gcaggcggta ttggtggagc cctttttttg ttgggagcga agatggtttc
     3121 tggtttcgat ttagtcagcc aaatcactgg attggaaaaa aagatcgaac aagcagatgt
     3181 catcattaca ggagaagggc ggatcgatca gcaaacgatc cacggaaaag ttccatacgg
     3241 tgtagcagtg cttgcaaaaa aatatcgaaa accaataatc gctatttgcg gaagtagaga
     3301 ccaagagcta gaaggacttt ctgattttct cccaattgtc ttaagtattc atttgggtcc
     3361 aataggttta gaagaagcaa tggaacacag gcaaacatta gaaaaaacaa aaatcttagg
     3421 acagacactt agcagatttt tcaccatcct cccgaagtag ttgacaaaat cattttcttt
     3481 atgctatcgt aatttcaaat ataggacaaa gtgagggatc cgtgcattga aaaattaagt
     3541 cgattgatga aagctgcatt cagactgtac cttgtggaga caaggaagca gtgtgcagat
     3601 tttcgggatt ggcttggttt tttcgatgta tgggtctttt tgtgtgtatc caacccttgc
     3661 atcctcttta gccagttctg aaaataggac tggtcttttt ttacgaggtg atggaaaatg
     3721 tctaaaatcg aaataaaaaa tctgacattc ggctacgaca gccaaggcac attattattt
     3781 gaacaagcaa atctaaattt tgacacacaa tggaaactag gacttatcgg acgaaacggt
     3841 cgaggaaaga caactttact gaatattcta caaaacaaac taccttatca agggcaagta
     3901 atccatcagc aagaatttgc ctatttcccg caacagacaa aagataaaga acgtttaacc
     3961 tattacgtgt taaatgatat tacggatttt gagatatggg aaatcgaaag agagctccaa
     4021 ttgatgcaaa cagatcctga aatcttatgg agagaattca gcacactatc ggggggagag
     4081 aagacaaaag tcctactggc acttttattt gtggatgaca ctcatttccc gttaatcgat
     4141 gaaccaacga atcatttgga tatctctggt agaaaacaag tagcggctta tttgaaaaag
     4201 aaaaaacaag gcttcatcgt ggtcagccat gaccggggat ttatcgatga agtagtggac
     4261 catgttttag caatcgaaaa aagtcaactg gaactttatc aagggaattt ctctatctat
     4321 gaagaacaga aaaaacttcg tgatgaattt gaaatggctc aaaatgaaaa attgaaaaaa
     4381 gaagtcagta ggctaaagaa aacagcagct gaaaaagccg aatggtctcg ttcccgagaa
     4441 ggagataaaa caaagaaaca agtcggattc atcgatactg aatctagacg agtgaataaa
     4501 ggagcagtgg gtgctgatgc tgcacggacg atgaaacgat ccaaagcaat cgtgaatcgg
     4561 atggagaccc agatcagcga gaaagaaaaa ctattaaaag atatcgaata tatcgattct
     4621 ttgacgatga atagccaagc gtctcaccat aagcgacttt taagcgtaga agatcttcaa
     4681 ttagggtatg aaaatctgtt attcgagcca attcatttta caatcgagcc tcatcagcgg
     4741 gtggcgattt caggtcctaa cggtgcagga aagtcatcca ttatccatta tcttctgggg
     4801 gcattcaacg gcaaggttat aggagaaaaa taccagccaa aacatctgag cattagttat
     4861 gcaagccaaa attatgaaga caatcgagga acgttggcgg aatttgcaga gaaaaaccaa
     4921 gtagactacc aagcattttt gaacaacctc cgaaagcttg ggatggaaag agatgttttt
     4981 cataacaaga tcgagcagat gagtatgggc caacggaaaa aagtggaatt ggctaaatct
     5041 ttatcacagc cagctgaact atatacatgg gatgaaccat tgaattattt ggatgtcttc
     5101 aatcaagaac aattagaaca actgatcttg aacgtgaaac ctgccatgtt actagtggaa
     5161 catgatcaaa ccttcctgga taaagtatct actgagatta tttctcttga gagaatctaa
     5221 cccttgtaat ctcaagaaca acaaggtatg gttaagggga actttgccaa cgaaggagag
     5281 aatcacatgc tttatgtaac gagacacgga gaaacaactt ggaacgcaca aggattagtt
     5341 tgcggacggg cagatgtgcc gctgactgaa aaaggacaga tgcaggcaca gaaactagca
     5401 gaaaaggtag tagatttgcc tgttccaatc acgaaaatca tccattcccc tcttcaacga
     5461 gcaagagata cagcacaagc agttgctgat cgtctcagct tgccgctgac cgttgatgaa
     5521 cgattggtag aaatggattt tggggattat gatggactgc caagcaaaga tgagaacttt
     5581 caaaaagcac gtttagcgtt cgctgtacga ttcccaaatg gggagtcagt cttagatgtg
     5641 tatgcacgga tcgttccttt attgaaagaa tgcatcgaag acgaagaaaa tgtctatctg
     5701 ttagtttgcc ataatgcctt gataagagtg atcaatgctt attttcatcc gatgcctaat
     5761 gaggggttct ttacattcat ggtagataat acagaattgg tttcttatga gtaattatgt
     5821 ttttgtgtta tttaaataaa atatttcaag gcactgcttt tgcttagcag tgcctttttt
//
LOCUS       DARBEG010000299         5614 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.128, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000299 DARBEG010000000
VERSION     DARBEG010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.128"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(41..1027)
                     /locus_tag="SCK20_003023"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="SCK20_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="SCK20_003024"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="SCK20_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1146995.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="SCK20_003025"
     CDS             1852..3006
                     /locus_tag="SCK20_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1146996.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="SCK20_003026"
     CDS             3221..4189
                     /locus_tag="SCK20_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1146997.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="SCK20_003027"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="SCK20_003027"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1146998.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5584
                     /locus_tag="SCK20_003028"
                     /pseudo
     CDS             5219..5584
                     /locus_tag="SCK20_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1263 g         1563 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcggttc
     5581 tgttgcaaag ttttaaataa agaataaaat ccct
//
LOCUS       DARBEG010000300         4223 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.147, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000300 DARBEG010000000
VERSION     DARBEG010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.147"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            173..1651
                     /gene="msr(C)"
                     /locus_tag="SCK20_003029"
     CDS             173..1651
                     /gene="msr(C)"
                     /locus_tag="SCK20_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1146999.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1698..2144
                     /locus_tag="SCK20_003030"
     CDS             1698..2144
                     /locus_tag="SCK20_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1147000.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2343..2858)
                     /locus_tag="SCK20_003031"
     CDS             complement(2343..2858)
                     /locus_tag="SCK20_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1147001.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2975..4177
                     /locus_tag="SCK20_003032"
     CDS             2975..4177
                     /locus_tag="SCK20_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1147002.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1257 a          712 c          920 g         1334 t
ORIGIN      
        1 tttttcgaac gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt
       61 tatttcgttc agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct
      121 cttttttagt atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa
      181 tttagcagta aatataacaa atctacaagt cagctttggc aaccagctag aactatctat
      241 tgattctctt cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg
      301 taaatcgact ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca
      361 aacagagatc accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct
      421 aaatttggaa ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg
      481 gagcggaggg gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg
      541 gatgcttcta gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga
      601 agagcttcgt tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga
      661 tgagctagca tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa
      721 ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga
      781 gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc
      841 ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt
      901 gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt
      961 agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg
     1021 ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt
     1081 ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg
     1141 taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat
     1201 ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca
     1261 aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga
     1321 gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt
     1381 gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct
     1441 ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc
     1501 aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc
     1561 tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa
     1621 aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt
     1681 taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga
     1741 tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc
     1801 atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg
     1861 tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa
     1921 gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta
     1981 tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata
     2041 tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc
     2101 cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt
     2161 gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag
     2221 tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt
     2281 gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc
     2341 ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc
     2401 tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct
     2461 agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct
     2521 ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt
     2581 gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca
     2641 agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc
     2701 aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga
     2761 tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat
     2821 aaaagcacgc ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta
     2881 ttttctttat tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa
     2941 ataaatcatg tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga
     3001 tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc
     3061 tacctgtatg atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc
     3121 tgcacagtta aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt
     3181 cactggtgtg gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac
     3241 attgatgaca ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa
     3301 tatcggaatg ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc
     3361 tactgcttta gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat
     3421 gtatgaagca gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg
     3481 cagtggttca tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat
     3541 agccctagcg gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga
     3601 aggtgtcaaa gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata
     3661 taatttcgga ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga
     3721 attagaagtc ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt
     3781 cgttgcaccg gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt
     3841 cttattcatt atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc
     3901 gatcagcatt gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat
     3961 tgctatggaa ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg
     4021 ctttttcggt ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga
     4081 aagctatgcg ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta
     4141 tcaccgtaat tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc
     4201 tgtcctgcat caagtcataa gaa
//
LOCUS       DARBEG010000301         1521 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.203, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000301 DARBEG010000000
VERSION     DARBEG010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.203"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..325)
                     /locus_tag="SCK20_003033"
     CDS             complement(<1..325)
                     /locus_tag="SCK20_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1147003.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVK"
     gene            complement(1039..1329)
                     /locus_tag="SCK20_003034"
     CDS             complement(1039..1329)
                     /locus_tag="SCK20_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1147004.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          483 a          296 c          209 g          533 t
ORIGIN      
        1 ttttaacatt tattttctca aaaacatact tgcaaagtaa tactacaatt cctaacataa
       61 tcgtagaatt taataatttc catagcataa agtgtttaga aaagaacatt aagaatagtt
      121 ctataataat tcttgaactc catgtcttat aatgttctat cgaagcaccc aaatagccaa
      181 gttcatctaa tctacttgca taaactacgt catccgctct catagtcggc atatacatat
      241 taaagagtaa aaagataccc caaatcccta ttagtaacaa gatcagttct ttgttattcg
      301 ttatttttct attatgtttg ttcattttta tactccttct tatatttcct aatagagtat
      361 aacatagctt taagcattac ttaaagataa cttaaaagtt atcttttaaa tacttaattg
      421 ttaaatacct tatttgatgc aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat
      481 atccagaaaa atttttctta gttaaggacc tatggattgc agtaatgcag aattcgtcga
      541 ataaactgcc aaagacatac aatcatattg cttttaaaat tgacgaacaa gatatagatt
      601 cgtttgtctc aaaaatccaa atgttaggtt taactgtcga accaggacgt tctcgtgtga
      661 aaggcgaggc ttcttcaatt tacttttacg actatgataa tcacctcttc gagttacata
      721 ccggtactct ccaggaacgt ttaaaatcct acaattccac tacctgagac tagtaatgac
      781 tagtctcttt ttatggcctc tgatcccccc attgagcgaa tactaatagg gcttcgaatt
      841 gatctgttca atctttaagc aatatccctc aaatgagttt ggaaagttta gtgattcatc
      901 acaaatactt aaatacatgt agttatatat agattgaggt tcttgtgagc gtatgctcgt
      961 gtgggttcaa atcccatcgt ctgcatccat ctttccaaaa caagcggcta ggacattttt
     1021 gtctcagccg cttgttttct agtttattct ttcatcttgt cggacctttt ttcttgggta
     1081 tagacttttc tcatattcta ccacaatttt gggtttccct aacacatgat aggatctcac
     1141 aacaaattgc tttctattat tataataacc tgtaacgact aacttcatcc cttcgtctac
     1201 ttcagctagg aagttcaaac tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa
     1261 acggatcaac ggtctggcac taacttgcaa agtacggatc ttatctactg ttccatattt
     1321 tttctccata accttcacct caataccatt atacgaacat acattccctt tgtaaagaag
     1381 aaactctgtt ttatcttttt gactttttgt ctattacctt tcttaaaatc atattggcac
     1441 ttcctgcgaa gatgaaggac atgtactaaa aaaaagaggc agcagtatag attactgttg
     1501 cttctttact aatttaggtt t
//
LOCUS       DARBEG010000302         1504 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.206, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000302 DARBEG010000000
VERSION     DARBEG010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.206"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            262..345
                     /locus_tag="SCK20_003035"
                     /pseudo
     CDS             262..345
                     /locus_tag="SCK20_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="SCK20_003036"
     CDS             394..477
                     /locus_tag="SCK20_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1147005.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="SCK20_003037"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="SCK20_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1147006.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DARBEG010000303         1350 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.216, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000303 DARBEG010000000
VERSION     DARBEG010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.216"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..298
                     /locus_tag="SCK20_003038"
                     /pseudo
     CDS             47..298
                     /locus_tag="SCK20_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="SCK20_003039"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="SCK20_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1147007.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DARBEG010000304          577 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4307
            SAMEA114569921-rid20112323.guided.275, whole genome shotgun
            sequence.
ACCESSION   DARBEG010000304 DARBEG010000000
VERSION     DARBEG010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569921
            Sequence Read Archive: ERR12203746
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972633.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972633.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:23:04
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,040
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 26 of 70
            Pseudo Genes (incomplete)         :: 32 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 6 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569921-rid20112323.guided.275"
                     /isolate="VRE4307"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>577
                     /locus_tag="SCK20_003040"
     CDS             <1..>577
                     /locus_tag="SCK20_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1147008.1"
                     /translation="RDFILYLKLCNRTESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQ
                     IYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANA
                     VILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMK
                     YLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNE"
BASE COUNT          231 a           53 c          105 g          188 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaat
//
